MD11G1047400.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0002466
Physical Location & Seq
Reverse (-)
4120208 .. 4124644
4437 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1047400.v1.1.491

Sequence Viewer

Length: 3621 bp
ATGATGGGATTTTTTTCCTTTCTGCTTTTGTTTGTTTTTCACTTCCACCTGCTCCTAAGTTTGACCTCAGGCGCCAGAATTTTGTCTTCAAGCAGCGGTGGCGGTGGCGGTGGTGATGTGGTGGAGCTGTTGTATGCATTCAAACAATCTTCTGTTGTATCTGATCCCCATGGCATCTTATCTGATTGGAAACCTGATTCCACCACTCCTCTCTGCTCTTGGAAAGGCATCACCTGCTCCTCCGACGGTGCAGTCACCTCCCTCAACCTCTCTAACGCCGGTCTCATTGGCGGCCTCCACCTTCCGCCTCTCGCCGCCCTGCCGAGCCTCCAGCAACTCCACCTGCAGGGCAATTATTTCTCTGCTGTTGATCTCTCTGTGTCCAATGTAACATCATGCAGACTTGAAACCGTTGACTTGTCCTCCAACAACATCTCCCAAGCGCTTCCAATCCGATCGTTTCTTCAGGGTTGCGACCGCCTTGTTTTCGCTAACCTGTCTCACAATTCAATCCCCGGAGGCGATCTAGGGTTTGGTGCTTCTCTGCTTCAGCTGGACATCTCTCACAATCTGATTTCGAATGCGGATTCGTTGACTTGCAACAGTCTGAACTTGCTCAATGTTTCACACAACAAGCTGACCGGAAAGTTGAGTGATTCTTTCTTGTCCTGCAAGAATGTATCAACCCTTGACCTTTCAAACAATGGCTTTTCCGGGGAATTACCGAACAGCTTCATTGCAAAGGCTTCAGCTTCTCTCGAGTATTTGGATCTCTCAAGCAACAATTTTTCGGGGACGTTCTCCGCCCTTGATTTCGGGCAGTGCGGCAGCCTCACTTTGCTTAGGCTATCACACAATGCACTCTCCGGGGATCAGTTTCCTCCGAGTTTCGAAAACTGTCAGGCTCTGGAGACGCTGGACCTCTCGAATAATAAGCTTGAGAACGAAATCCCGGGTGTTTTGTTGGGAAATCTCAAGAAATTGAGGCAACTATTTCTTGGTCACAACTATTTTTCGGGTGCAATTCCAGCCGAACTAGGAAAAGCTTGTGGGACTCTTCAGGAACTTGATATTTCTGGTAACATTCTCTCTGGAGAGTTCCCTTCGAGTTTTTTGTCCTGCACTTCATTGGTTAGCCTCAATCTCGGACACAATCAGCTCTCAGGGAATTTTCTCAATACCGTTGTAAGCCGTCTTCCGAGCTTGAGATATCTCTATGTACCATTCAACAACATAACTGGTTCTGTGCCGCTGTCTCTTACCAATGGCACGCGGCTTCAAGTGCTTGATCTCAGTTCGAACATCTTCACTGGGAACATTCCTTCAGGGTTTTGCTCCTCCAATGCTGCATCAGTTTTAGAAAAAGTGCTTCTAGCTAACAATTTGCTGTCCGGGAATGTGCCCTCGGAGCTTGGAAACTGCAAGAACCTGAATTCCATTGATCTGAGCTTCAACCACTTGAGTGGTCCAATTCCTTTAGAAATTTGGAGCTTGCCGAAGCTCTCTGACTTGGTTATGTGGGCAAACAACCTCACCGGTGAAATCCCAGAAGGCATTTGCATTGATGGAGGAAACCTGGAAACTCTGATTCTCAACAACAATCTCATCACTGGAACCATTCCGCGGTCGATTGTCAACTGCACCAACATGATTTGGGTGTCACTATCTGGTAACCGCCTTAGCGGAGATATCCCATCTGGCATTGGGAATCTCCATAAGCTTGCTATACTCCAGTTAGGCAACAATTCGCTCTCGGGACAGATTCCAGCTGAGCTTGGCAAGTGTGAGAACCTCATTTGGCTTGATTTGAACAGCAATGACCTAAGTGGTTCTATCCCACCAGAGCTGTCCAACCAAGCCGGCCTAGTCCTTCCTGGGATTGTTTCCGGGAAGCAGTTTGCATTCGTAAGAAATGAGGGTGGGACATCTTGCAGGGGTGCAGGAGGACTAGTTGAGTTTGAGGGAATCCGAGCAGAAACACTAGAAAAGTTTCCTATGGTTCACTCGTGCTCGTCGACTAGAATTTACTCTGGATGGACAGTTTACACATTCACCAGCAATGGCAGCATGATCTTCCTTGATCTATCCTACAACTCCTTGACTGGAACCATTCCTGAAAACTTAGGTATGCTGTCCTACGTACAAGTCCTTAACCTAGGACACAACAAGCTATCCGGAAACATTCCTGATAGCTTTGGAGGATTGAAAGCAGTTGGAGTCCTTGATCTCTCTCATAACAACCTTCAGGGTTTTGTCCCAGGATCGTTGGGGACTCTCTCTTTTCTGAATGACCTTGATGTGTCTAACAACAACCTCACCGGTCCCATCCCTTCCGGAGGTCAGCTGACCACTTTCCCAGCATCTAGATATGAGAACAACTCCGGCCTTTGCGGGGTACCCTTGGCGGCCTGTAGCTCTCAAAGGCACTCAACAGATTCCAAAGTTGGAGGGAGAAATAATTCTTTGACTTCAGCAATGGTAATTGGCGTCACGTTCTTTTTCTTCTGTATCCTTATACTTATGTTAATGCTGTATCGGGTGAAGAAGTACCAGCTGAAGGAGGAAAAGAGAGAGAAATATATCGAAAGCCTTCCAACTTCCGGTGGCAGCAGCTGGAAACTTTCCAGTGTTCCCGAGCCTCTGAGCATCAATGTAGCCACATTTGAGAAGCCTTTGAGGAAACTGACCTTCGCCCATCTGCTCGAAGCCACTAATGGTTTCAGTGCAGACAGCTTGATAGGCAGTGGAGGATTTGGCGAGGTCTACAAGGCACAACTGGGAGATGGCTCTGTTGTTGCAATTAAGAAGCTAATCCAAGTCACAGGCCAAGGTGACAGGGAGTTTATGGCAGAAATGGAAACTATTGGGAAAATCAAGCACCGAAACCTGGTTCCTTTGTTAGGGTACTGTAAGATCGGCGAGGAGAGACTTCTCGTGTATGAGTACATGAAATGGGGAAGTCTGGAGGCTGTTCTTCATGACAAATCCAAGGCAGGGGTTTCAAGGCTTGATTGGGCAGCAAGAAAGAAGATTACTATAGGTTCCGCGAGAGGTCTAGCTTTCCTTCACCATAGTTGCATACCTCATATTATCCACCGTGACATGAAGTCTAGCAATGTTCTTCTGGATGAAAATTTTGAGGCCAGAGTATCTGATTTTGGCATGGCAAGATTGGTCAATGCCCTTGATACTCATCTCAGCGTGAGTACCCTTGCAGGCACTCCGGGTTATGTTCCCCCCGAGTACTACCAGAGTTTTCGATGCACAACAAAAGGGGATGTCTACAGCTACGGTGTTATTCTGCTAGAGCTTCTCTCGGGGAAAAGGCCTATAGATCCGTCAGCATTTGGGGATGACAACAACCTGGTTGGATGGGCAAAGCAGCTTCAGAGAGAGAAGAGATGGAATGAGATACTGGACGCCGAGCTGTTGACGCAGATATCTGGTGAGGCTGAATTATACCAGTATCTGAGGATTGCCTTTGAGTGCCTTGATGACAGGCCATTCCGAAGACCAAACATGATCCAAGTTATGGCTATGTTTAAAGAGCTTCAGGTTGGTTCAGAAAATGACGTCCTCGATGGCTTTTCGCTGAAAGAGACTGTTGTTGAGGAACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase

Protein Analysis

1207

Amino Acids

130.11

Weight (kDa)

5.68

Isoelectric Point (pI)

34.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 43 - 80 1e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 294 - 407 2.2e-06 Leucine-rich repeat region
LRR_8 PF13855 296 - 337 5e-06 Leucine rich repeat
LRR_8 PF13855 352 - 412 9.9e-08 Leucine rich repeat
Island PF20141 621 - 685 2.6e-31 Brassinosteroid receptor island domain
LRR_8 PF13855 714 - 772 6.6e-09 Leucine rich repeat
Pkinase PF00069 909 - 1178 8.4e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 910 - 1178 1.4e-41 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 57, 242, 351
AasI GACNNNNNNGTC 1 cut(s) 251
AatII GACGTC 1 cut(s) 3576
Acc36I ACCTGC 3 cut(s) 57, 242, 351
Acc65I GGTACC 1 cut(s) 2395
AccB1I GGYRCC 2 cut(s) 71, 2395
AccB7I CCANNNNNTGG 1 cut(s) 3532
AccI GTMKAC 3 cut(s) 2015, 2763, 3282
AccII CGCG 3 cut(s) 1273, 1624, 3047
AccIII TCCGGA 2 cut(s) 2174, 2333
AclWI GGATC 6 cut(s) 158, 777, 879, 2269, 3329, 3517
AcsI RAATTY 6 cut(s) 78, 1168, 1432, 1482, 2022, 3133
AcyI GRCGYC 4 cut(s) 72, 2487, 3420, 3573
AfaI GTAC 8 cut(s) 1221, 2142, 2397, 2549, 2906, 2945, 3208, 3245
AfeI AGCGCT 1 cut(s) 444
AgeI ACCGGT 2 cut(s) 1535, 2318
AhdI GACNNNNNGTC 1 cut(s) 3106
AhlI ACTAGT 1 cut(s) 1948
AjnI CCWGG 5 cut(s) 1575, 1873, 2257, 2886, 3363
AleI CACNNNNGTG 1 cut(s) 1461
AloI GAACNNNNNNTCC 2 cut(s) 1418, 1450
Alw21I GWGCWC 1 cut(s) 2012
Alw26I GTCTC 6 cut(s) 287, 504, 905, 1260, 2920, 3593
AlwI GGATC 6 cut(s) 158, 777, 879, 2269, 3329, 3517
AlwNI CAGNNNCTG 3 cut(s) 907, 2613, 3469
Ama87I CYCGRG 6 cut(s) 758, 952, 1753, 2633, 3239, 3316
Aor13HI TCCGGA 2 cut(s) 2174, 2333
Aor51HI AGCGCT 1 cut(s) 444
AoxI GGCC 8 cut(s) 292, 1861, 2383, 2406, 2824, 3141, 3326, 3500
ApeKI GCWGC 8 cut(s) 93, 828, 1346, 2064, 2607, 2610, 3017, 3382
ApoI RAATTY 6 cut(s) 78, 1168, 1432, 1482, 2022, 3133
AsiGI ACCGGT 2 cut(s) 1535, 2318
Asp700I GAANNNNTTC 5 cut(s) 731, 1304, 1989, 2458, 2589
Asp718I GGTACC 1 cut(s) 2395
AspA2I CCTAGG 1 cut(s) 2155
AspLEI GCGC 2 cut(s) 74, 445
AspS9I GGNCC 3 cut(s) 919, 1466, 2321
AsuC2I CCSGG 8 cut(s) 516, 715, 868, 953, 954, 1393, 1888, 3225
AsuII TTCGAA 3 cut(s) 578, 891, 1298
AvaI CYCGRG 6 cut(s) 758, 952, 1753, 2633, 3239, 3316
AvaII GGWCC 3 cut(s) 919, 1466, 2321
AvrII CCTAGG 1 cut(s) 2155
AxyI CCTNAGG 1 cut(s) 67
BaeGI GKGCMC 1 cut(s) 1404
BanI GGYRCC 2 cut(s) 71, 2395
BarI GAAGNNNNNNTAC 2 cut(s) 1179, 1211
BauI CACGAG 2 cut(s) 2005, 2933
BbsI GAAGAC 3 cut(s) 78, 1187, 3517
Bbv12I GWGCWC 1 cut(s) 2012
BbvI GCAGC 8 cut(s) 105, 840, 1333, 2076, 2619, 2622, 3029, 3394
BccI CCATC 9 cut(s) 1559, 1702, 2028, 2333, 2703, 2777, 3366, 3396, 3575
BceAI ACGGC 1 cut(s) 1176
BciT130I CCWGG 5 cut(s) 1577, 1875, 2259, 2888, 3365
BciVI GTATCC 1 cut(s) 2519
BcnI CCSGG 8 cut(s) 516, 715, 868, 953, 954, 1393, 1888, 3225
BcoDI GTCTC 6 cut(s) 287, 504, 905, 1260, 2920, 3593
BcuI ACTAGT 1 cut(s) 1948
BfmI CTRYAG 5 cut(s) 344, 2410, 3036, 3283, 3330
BfoI RGCGCY 2 cut(s) 75, 446
BfuAI ACCTGC 3 cut(s) 57, 242, 351
BfuI GTATCC 1 cut(s) 2519
BlnI CCTAGG 1 cut(s) 2155
BlpI GCTNAGC 1 cut(s) 1770
BmcAI AGTACT 1 cut(s) 3245
Bme18I GGWCC 3 cut(s) 919, 1466, 2321
BmeRI GACNNNNNGTC 1 cut(s) 3106
BmeT110I CYCGRG 6 cut(s) 758, 952, 1753, 2633, 3239, 3316
BmgT120I GGNCC 3 cut(s) 919, 1466, 2321
BmiI GGNNCC 8 cut(s) 73, 1615, 2107, 2323, 2397, 2892, 3043, 3615
BmrI ACTGGG 2 cut(s) 1320, 2786
BmsI GCATC 6 cut(s) 183, 237, 1358, 2369, 2655, 3251
BmuI ACTGGG 2 cut(s) 1320, 2786
BpiI GAAGAC 3 cut(s) 78, 1187, 3517
BplI GAGNNNNNCTC 4 cut(s) 2363, 2395, 3299, 3331
BpmI CTGGAG 5 cut(s) 314, 929, 1113, 1715, 2984
Bpu10I CCTNAGC 2 cut(s) 842, 1679
Bpu1102I GCTNAGC 1 cut(s) 1770
Bpu14I TTCGAA 3 cut(s) 578, 891, 1298
BpuEI CTTGAG 5 cut(s) 760, 959, 959, 1225, 1480
BpuMI CCSGG 8 cut(s) 516, 715, 868, 953, 954, 1393, 1888, 3225
BsaAI YACGTR 1 cut(s) 2140
BsaBI GATNNNNATC 1 cut(s) 3192
BsaHI GRCGYC 4 cut(s) 72, 2487, 3420, 3573
BsaI GGTCTC 1 cut(s) 287
BsaWI WCCGGW 6 cut(s) 641, 1535, 2174, 2318, 2333, 2600
BsaXI ACNNNNNCTCC 8 cut(s) 116, 146, 407, 419, 437, 449, 1935, 1965
Bse118I RCCGGY 4 cut(s) 278, 1535, 1859, 2318
Bse1I ACTGG 9 cut(s) 1243, 1315, 1615, 1732, 2107, 2625, 2781, 3420, 3463
Bse21I CCTNAGG 1 cut(s) 67
Bse3DI GCAATG 5 cut(s) 735, 1822, 2065, 2481, 3121
Bse8I GATNNNNATC 1 cut(s) 3192
BseAI TCCGGA 2 cut(s) 2174, 2333
BseBI CCWGG 5 cut(s) 1577, 1875, 2259, 2888, 3365
BseGI GGATG 6 cut(s) 2039, 2325, 3133, 3283, 3358, 3377
BseJI GATNNNNATC 1 cut(s) 3192
BseMI GCAATG 5 cut(s) 735, 1822, 2065, 2481, 3121
BseMII CTCAG 8 cut(s) 81, 1176, 1306, 1436, 1761, 2633, 3211, 3461
BseNI ACTGG 9 cut(s) 1243, 1315, 1615, 1732, 2107, 2625, 2781, 3420, 3463
BseRI GAGGAG 4 cut(s) 198, 229, 1327, 2936
BseSI GKGCMC 1 cut(s) 1404
BseXI GCAGC 8 cut(s) 105, 840, 1333, 2076, 2619, 2622, 3029, 3394
BseYI CCCAGC 1 cut(s) 2356
BsgI GTGCAG 5 cut(s) 270, 1105, 1624, 1959, 2745
Bsh1236I CGCG 3 cut(s) 1273, 1624, 3047
Bsh1285I CGRYCG 3 cut(s) 458, 478, 1628
BshFI GGCC 8 cut(s) 294, 1863, 2385, 2408, 2826, 3143, 3328, 3502
BshNI GGYRCC 2 cut(s) 71, 2395
BshTI ACCGGT 2 cut(s) 1535, 2318
BsiEI CGRYCG 3 cut(s) 458, 478, 1628
BsiHKAI GWGCWC 1 cut(s) 2012
BsiHKCI CYCGRG 6 cut(s) 758, 952, 1753, 2633, 3239, 3316
BslFI GGGAC 7 cut(s) 808, 1066, 1770, 1936, 2240, 2284, 2307
BsmAI GTCTC 6 cut(s) 287, 504, 905, 1260, 2920, 3593
BsmBI CGTCTC 1 cut(s) 905
BsmFI GGGAC 7 cut(s) 808, 1066, 1770, 1936, 2240, 2284, 2307
BsmI GAATGC 3 cut(s) 137, 586, 1901
BsnI GGCC 8 cut(s) 294, 1863, 2385, 2408, 2826, 3143, 3328, 3502
Bso31I GGTCTC 1 cut(s) 287
BsoBI CYCGRG 6 cut(s) 758, 952, 1753, 2633, 3239, 3316
Bsp119I TTCGAA 3 cut(s) 578, 891, 1298
Bsp1286I GDGCHC 2 cut(s) 1404, 2012
Bsp13I TCCGGA 2 cut(s) 2174, 2333
Bsp1720I GCTNAGC 1 cut(s) 1770
Bsp19I CCATGG 1 cut(s) 169
BspANI GGCC 8 cut(s) 294, 1863, 2385, 2408, 2826, 3143, 3328, 3502
BspCNI CTCAG 8 cut(s) 80, 1175, 1305, 1437, 1762, 2634, 3210, 3462
BspEI TCCGGA 2 cut(s) 2174, 2333
BspFNI CGCG 3 cut(s) 1273, 1624, 3047
BspHI TCATGA 1 cut(s) 2977
BspLI GGNNCC 8 cut(s) 73, 1615, 2107, 2323, 2397, 2892, 3043, 3615
BspMAI CTGCAG 1 cut(s) 348
BspMI ACCTGC 3 cut(s) 57, 242, 351
BspPI GGATC 6 cut(s) 158, 777, 879, 2269, 3329, 3517
BspT104I TTCGAA 3 cut(s) 578, 891, 1298
BspT107I GGYRCC 2 cut(s) 71, 2395
BspTNI GGTCTC 1 cut(s) 287
BsrDI GCAATG 5 cut(s) 735, 1822, 2065, 2481, 3121
BsrFI RCCGGY 4 cut(s) 278, 1535, 1859, 2318
BsrI ACTGG 9 cut(s) 1243, 1315, 1615, 1732, 2107, 2625, 2781, 3420, 3463
BssAI RCCGGY 4 cut(s) 278, 1535, 1859, 2318
BssNI GRCGYC 4 cut(s) 72, 2487, 3420, 3573
BssSI CACGAG 2 cut(s) 2005, 2933
BssT1I CCWWGG 5 cut(s) 169, 2155, 2400, 2827, 2988
Bst2BI CACGAG 2 cut(s) 2005, 2933
Bst2UI CCWGG 5 cut(s) 1577, 1875, 2259, 2888, 3365
Bst6I CTCTTC 2 cut(s) 1062, 3392
BstACI GRCGYC 4 cut(s) 72, 2487, 3420, 3573
BstAPI GCANNNNNTGC 1 cut(s) 234
BstBAI YACGTR 1 cut(s) 2140
BstBI TTCGAA 3 cut(s) 578, 891, 1298
BstC8I GCNNGC 5 cut(s) 1271, 1493, 1722, 1861, 3217
BstDSI CCRYGG 2 cut(s) 169, 1622
BstEII GGTNACC 1 cut(s) 1670
BstENI CCTNNNNNAGG 2 cut(s) 2334, 2898
BstF5I GGATG 6 cut(s) 2039, 2325, 3133, 3283, 3358, 3377
BstFNI CGCG 3 cut(s) 1273, 1624, 3047
BstH2I RGCGCY 2 cut(s) 75, 446
BstHHI GCGC 2 cut(s) 74, 445
BstMAI GTCTC 6 cut(s) 287, 504, 905, 1260, 2920, 3593
BstMCI CGRYCG 3 cut(s) 458, 478, 1628
BstMWI GCNNNNNNNGC 8 cut(s) 99, 234, 1028, 1282, 1408, 2064, 2739, 3433
BstNI CCWGG 5 cut(s) 1577, 1875, 2259, 2888, 3365
BstPI GGTNACC 1 cut(s) 1670
BstSFI CTRYAG 5 cut(s) 344, 2410, 3036, 3283, 3330
BstSLI GKGCMC 1 cut(s) 1404
BstSNI TACGTA 1 cut(s) 2140
BstUI CGCG 3 cut(s) 1273, 1624, 3047
BstV1I GCAGC 8 cut(s) 105, 840, 1333, 2076, 2619, 2622, 3029, 3394
BstV2I GAAGAC 3 cut(s) 78, 1187, 3517
BstX2I RGATCY 2 cut(s) 769, 3334
BstXI CCANNNNNNTGG 1 cut(s) 1463
BstYI RGATCY 2 cut(s) 769, 3334
Bsu36I CCTNAGG 1 cut(s) 67
BsuI GTATCC 1 cut(s) 2519
BsuRI GGCC 8 cut(s) 294, 1863, 2385, 2408, 2826, 3143, 3328, 3502
BtgI CCRYGG 2 cut(s) 169, 1622
BtsCI GGATG 6 cut(s) 2039, 2325, 3133, 3283, 3358, 3377
BtsI GCAGTG 2 cut(s) 827, 2749
BtsIMutI CAGTG 6 cut(s) 827, 1308, 1608, 2632, 2728, 2749
BveI ACCTGC 3 cut(s) 57, 242, 351
Cac8I GCNNGC 5 cut(s) 1271, 1493, 1722, 1861, 3217
CaiI CAGNNNCTG 3 cut(s) 907, 2613, 3469
CciI TCATGA 1 cut(s) 2977
CfoI GCGC 2 cut(s) 74, 445
Cfr10I RCCGGY 4 cut(s) 278, 1535, 1859, 2318
Cfr13I GGNCC 3 cut(s) 919, 1466, 2321
Cfr42I CCGCGG 1 cut(s) 1625
Cfr9I CCCGGG 1 cut(s) 952
CseI GACGC 4 cut(s) 922, 2476, 3428, 3442
CsiI ACCWGGT 2 cut(s) 2886, 3363
Csp6I GTAC 8 cut(s) 1220, 2141, 2396, 2548, 2905, 2944, 3207, 3244
CspAI ACCGGT 2 cut(s) 1535, 2318
CviAII CATG 9 cut(s) 170, 396, 1648, 2068, 2947, 2978, 3103, 3163, 3520
CviQI GTAC 8 cut(s) 1220, 2141, 2396, 2548, 2905, 2944, 3207, 3244
DinI GGCGCC 1 cut(s) 73
DraI TTTAAA 1 cut(s) 3544
DrdI GACNNNNNNGTC 1 cut(s) 251
DriI GACNNNNNGTC 1 cut(s) 3106
DseDI GACNNNNNNGTC 1 cut(s) 251
Eam1104I CTCTTC 2 cut(s) 1062, 3392
Eam1105I GACNNNNNGTC 1 cut(s) 3106
EarI CTCTTC 2 cut(s) 1062, 3392
EciI GGCGGA 2 cut(s) 294, 793
Eco105I TACGTA 1 cut(s) 2140
Eco130I CCWWGG 5 cut(s) 169, 2155, 2400, 2827, 2988
Eco147I AGGCCT 1 cut(s) 3328
Eco31I GGTCTC 1 cut(s) 287
Eco32I GATATC 3 cut(s) 1211, 1690, 3441
Eco47I GGWCC 3 cut(s) 919, 1466, 2321
Eco47III AGCGCT 1 cut(s) 444
Eco81I CCTNAGG 1 cut(s) 67
Eco88I CYCGRG 6 cut(s) 758, 952, 1753, 2633, 3239, 3316
Eco91I GGTNACC 1 cut(s) 1670
EcoNI CCTNNNNNAGG 2 cut(s) 2334, 2898
EcoO65I GGTNACC 1 cut(s) 1670
EcoRI GAATTC 1 cut(s) 1432
EcoRII CCWGG 5 cut(s) 1575, 1873, 2257, 2886, 3363
EcoRV GATATC 3 cut(s) 1211, 1690, 3441
EcoT14I CCWWGG 5 cut(s) 169, 2155, 2400, 2827, 2988
EcoT22I ATGCAT 1 cut(s) 139
EgeI GGCGCC 1 cut(s) 73
EheI GGCGCC 1 cut(s) 73
ErhI CCWWGG 5 cut(s) 169, 2155, 2400, 2827, 2988
Esp3I CGTCTC 1 cut(s) 905
FaeI CATG 9 cut(s) 173, 399, 1651, 2071, 2950, 2981, 3106, 3166, 3523
FaqI GGGAC 7 cut(s) 808, 1066, 1770, 1936, 2240, 2284, 2307
FatI CATG 9 cut(s) 169, 395, 1647, 2067, 2946, 2977, 3102, 3162, 3519
FauI CCCGC 1 cut(s) 2384
FblI GTMKAC 3 cut(s) 2015, 2763, 3282
FokI GGATG 6 cut(s) 2046, 2312, 3140, 3290, 3365, 3384
GlaI GCGC 2 cut(s) 73, 444
GsaI CCCAGC 1 cut(s) 2360
GsuI CTGGAG 5 cut(s) 314, 929, 1113, 1715, 2984
HaeII RGCGCY 2 cut(s) 75, 446
HaeIII GGCC 8 cut(s) 294, 1863, 2385, 2408, 2826, 3143, 3328, 3502
HgaI GACGC 4 cut(s) 922, 2476, 3428, 3442
HhaI GCGC 2 cut(s) 74, 445
Hin1I GRCGYC 4 cut(s) 72, 2487, 3420, 3573
Hin1II CATG 9 cut(s) 173, 399, 1651, 2071, 2950, 2981, 3106, 3166, 3523
Hin6I GCGC 2 cut(s) 72, 443
HinP1I GCGC 2 cut(s) 72, 443
HincII GTYRAC 5 cut(s) 415, 594, 1636, 2016, 3432
HindII GTYRAC 5 cut(s) 415, 594, 1636, 2016, 3432
HindIII AAGCTT 3 cut(s) 935, 1044, 1718
Hpy166II GTNNAC 9 cut(s) 415, 594, 1636, 2002, 2016, 2044, 2764, 3283, 3432
Hpy8I GTNNAC 9 cut(s) 415, 594, 1636, 2002, 2016, 2044, 2764, 3283, 3432
Hpy99I CGWCG 2 cut(s) 248, 2017
HpyCH4IV ACGT 4 cut(s) 797, 2139, 2492, 3573
HpyF10VI GCNNNNNNNGC 8 cut(s) 99, 234, 1028, 1282, 1408, 2064, 2739, 3433
HpySE526I ACGT 4 cut(s) 797, 2139, 2492, 3573
Hsp92I GRCGYC 4 cut(s) 72, 2487, 3420, 3573
Hsp92II CATG 9 cut(s) 173, 399, 1651, 2071, 2950, 2981, 3106, 3166, 3523
HspAI GCGC 2 cut(s) 72, 443
KasI GGCGCC 1 cut(s) 71
Kpn2I TCCGGA 2 cut(s) 2174, 2333
KpnI GGTACC 1 cut(s) 2399
KroI GCCGGC 1 cut(s) 1859
KroNI GCCGGC 1 cut(s) 1861
KspI CCGCGG 1 cut(s) 1625
LmnI GCTCC 6 cut(s) 57, 124, 242, 1340, 1408, 1488
Lsp1109I GCAGC 8 cut(s) 105, 840, 1333, 2076, 2619, 2622, 3029, 3394
LweI GCATC 6 cut(s) 183, 237, 1358, 2369, 2655, 3251
MabI ACCWGGT 2 cut(s) 2886, 3363
MaeII ACGT 4 cut(s) 797, 2139, 2492, 3573
MflI RGATCY 2 cut(s) 769, 3334
MhlI GDGCHC 2 cut(s) 1404, 2012
Mly113I GGCGCC 1 cut(s) 72
MlyI GAGTC 3 cut(s) 1048, 2226, 2266
MmeI TCCRAC 7 cut(s) 267, 450, 1875, 2194, 2425, 2618, 3349
Mph1103I ATGCAT 1 cut(s) 139
MroI TCCGGA 2 cut(s) 2174, 2333
MroNI GCCGGC 1 cut(s) 1859
MroXI GAANNNNTTC 5 cut(s) 731, 1304, 1989, 2458, 2589
MseI TTAA 4 cut(s) 2151, 2525, 2802, 3543
MslI CAYNNNNRTG 2 cut(s) 1461, 1646
MspA1I CMGCKG 8 cut(s) 96, 553, 1252, 1624, 1769, 2344, 2554, 2613
Mva1269I GAATGC 3 cut(s) 137, 586, 1901
MvaI CCWGG 5 cut(s) 1577, 1875, 2259, 2888, 3365
MvnI CGCG 3 cut(s) 1273, 1624, 3047
MwoI GCNNNNNNNGC 8 cut(s) 99, 234, 1028, 1282, 1408, 2064, 2739, 3433
NaeI GCCGGC 1 cut(s) 1861
NarI GGCGCC 1 cut(s) 72
NciI CCSGG 8 cut(s) 516, 715, 868, 953, 954, 1393, 1888, 3225
NcoI CCATGG 1 cut(s) 169
NgoMIV GCCGGC 1 cut(s) 1859
NlaIII CATG 9 cut(s) 173, 399, 1651, 2071, 2950, 2981, 3106, 3166, 3523
NlaIV GGNNCC 8 cut(s) 73, 1615, 2107, 2323, 2397, 2892, 3043, 3615
NmeAIII GCCGAG 2 cut(s) 348, 3448
NmuCI GTSAC 7 cut(s) 253, 1001, 1659, 2488, 2818, 2831, 3098
NsiI ATGCAT 1 cut(s) 139
NspV TTCGAA 3 cut(s) 578, 891, 1298
OliI CACNNNNGTG 1 cut(s) 1461
PaeR7I CTCGAG 1 cut(s) 758
PagI TCATGA 1 cut(s) 2977
PaqCI CACCTGC 3 cut(s) 57, 242, 351
PceI AGGCCT 1 cut(s) 3328
PcsI WCGNNNNNNNCGW 1 cut(s) 2012
PctI GAATGC 3 cut(s) 137, 586, 1901
PdiI GCCGGC 1 cut(s) 1861
PdmI GAANNNNTTC 5 cut(s) 731, 1304, 1989, 2458, 2589
PfeI GAWTC 8 cut(s) 197, 587, 656, 1588, 1708, 1762, 1965, 2435
PflMI CCANNNNNTGG 1 cut(s) 3532
PfoI TCCNGGA 2 cut(s) 1391, 1886
PinAI ACCGGT 2 cut(s) 1535, 2318
Ple19I CGATCG 1 cut(s) 458
PleI GAGTC 3 cut(s) 1048, 2225, 2266
PluTI GGCGCC 1 cut(s) 75
PpsI GAGTC 3 cut(s) 1048, 2225, 2266
Ppu21I YACGTR 1 cut(s) 2140
Psp6I CCWGG 5 cut(s) 1575, 1873, 2257, 2886, 3363
PspEI GGTNACC 1 cut(s) 1670
PspFI CCCAGC 1 cut(s) 2356
PspGI CCWGG 5 cut(s) 1575, 1873, 2257, 2886, 3363
PspN4I GGNNCC 8 cut(s) 73, 1615, 2107, 2323, 2397, 2892, 3043, 3615
PspPI GGNCC 3 cut(s) 919, 1466, 2321
PstI CTGCAG 1 cut(s) 348
PstNI CAGNNNCTG 3 cut(s) 907, 2613, 3469
PsuI RGATCY 2 cut(s) 769, 3334
PvuI CGATCG 1 cut(s) 458
PvuII CAGCTG 5 cut(s) 553, 1769, 2344, 2554, 2613
RsaI GTAC 8 cut(s) 1221, 2142, 2397, 2549, 2906, 2945, 3208, 3245
RsaNI GTAC 8 cut(s) 1220, 2141, 2396, 2548, 2905, 2944, 3207, 3244
RseI CAYNNNNRTG 2 cut(s) 1461, 1646
SacII CCGCGG 1 cut(s) 1625
SalI GTCGAC 1 cut(s) 2014
SaqAI TTAA 4 cut(s) 2151, 2525, 2802, 3543
Sau96I GGNCC 3 cut(s) 919, 1466, 2321
SbfI CCTGCAGG 1 cut(s) 348
ScaI AGTACT 1 cut(s) 3245
SchI GAGTC 3 cut(s) 1048, 2226, 2266
SdaI CCTGCAGG 1 cut(s) 348
SduI GDGCHC 2 cut(s) 1404, 2012
SexAI ACCWGGT 2 cut(s) 2886, 3363
SfaNI GCATC 6 cut(s) 183, 237, 1358, 2369, 2655, 3251
SfcI CTRYAG 5 cut(s) 344, 2410, 3036, 3283, 3330
SfoI GGCGCC 1 cut(s) 73
Sfr274I CTCGAG 1 cut(s) 758
Sfr303I CCGCGG 1 cut(s) 1625
SfuI TTCGAA 3 cut(s) 578, 891, 1298
SgrAI CRCCGGYG 1 cut(s) 1535
SgrBI CCGCGG 1 cut(s) 1625
SinI GGWCC 3 cut(s) 919, 1466, 2321
SlaI CTCGAG 1 cut(s) 758
SmaI CCCGGG 1 cut(s) 954
SmiMI CAYNNNNRTG 2 cut(s) 1461, 1646
SmlI CTYRAG 6 cut(s) 758, 775, 938, 974, 1204, 1459
SmoI CTYRAG 6 cut(s) 758, 775, 938, 974, 1204, 1459
SnaBI TACGTA 1 cut(s) 2140
SpeI ACTAGT 1 cut(s) 1948
Sse8387I CCTGCAGG 1 cut(s) 348
SseBI AGGCCT 1 cut(s) 3328
SspDI GGCGCC 1 cut(s) 71
StuI AGGCCT 1 cut(s) 3328
StyI CCWWGG 5 cut(s) 169, 2155, 2400, 2827, 2988
TaiI ACGT 4 cut(s) 800, 2142, 2495, 3576
TatI WGTACW 2 cut(s) 2943, 3243
TauI GCSGC 6 cut(s) 294, 317, 828, 1252, 1276, 2408
TfiI GAWTC 8 cut(s) 197, 587, 656, 1588, 1708, 1762, 1965, 2435
Tru1I TTAA 4 cut(s) 2151, 2525, 2802, 3543
Tru9I TTAA 4 cut(s) 2151, 2525, 2802, 3543
TscAI CASTG 6 cut(s) 827, 1315, 1615, 2632, 2728, 2749
TseFI GTSAC 7 cut(s) 253, 1001, 1659, 2488, 2818, 2831, 3098
TseI GCWGC 8 cut(s) 93, 828, 1346, 2064, 2607, 2610, 3017, 3382
Tsp45I GTSAC 7 cut(s) 253, 1001, 1659, 2488, 2818, 2831, 3098
TspDTI ATGAA 6 cut(s) 724, 1116, 2963, 2966, 3119, 3144
TspGWI ACGGA 1 cut(s) 3327
TspMI CCCGGG 1 cut(s) 952
TspRI CASTG 6 cut(s) 827, 1315, 1615, 2632, 2728, 2749
Van91I CCANNNNNTGG 1 cut(s) 3532
VpaK11BI GGWCC 3 cut(s) 919, 1466, 2321
XagI CCTNNNNNAGG 2 cut(s) 2334, 2898
XapI RAATTY 6 cut(s) 78, 1168, 1432, 1482, 2022, 3133
XbaI TCTAGA 1 cut(s) 2363
XhoI CTCGAG 1 cut(s) 758
XmaI CCCGGG 1 cut(s) 952
XmaJI CCTAGG 1 cut(s) 2155
XmiI GTMKAC 3 cut(s) 2015, 2763, 3282
XmnI GAANNNNTTC 5 cut(s) 731, 1304, 1989, 2458, 2589
ZraI GACGTC 1 cut(s) 3574
ZrmI AGTACT 1 cut(s) 3245
Zsp2I ATGCAT 1 cut(s) 139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.