Rroxscaffold_1G00007140
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0002466
Physical Location & Seq
Reverse (-)
9133854 .. 9137430
3577 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00007140.1

Sequence Viewer

Length: 3534 bp
ATGATGGGTTTGTTCTGTTTTCTGGTTCTGCTTCTTTTTCACTCACTAAATTTCTCTTCAGCTGCTTCTAATGAGGATGTGGTGAAGAAGCTTTTGTTGCAGTTCAAGCAATCCTCTGTTCAATCTGACCCTCAAGGTTTCTTATCTGACTGGAAATCTGATTCTTCAACTGCTCTTTGTTCATGGAAAGGCCTGACTTGCTCTGAAGGCCACGTCATCACCCTCGACCTCTCCTATTCTGGTCTCATTGGAAGTCTTCACCTTCCAACTCTGACAGCCTTGCCGAGTCTTCAAAATCTGTACTTGCAAGGCAATTCATTCTCTGCTTTTGATCTTTCAGTTTCCAACATTACCTCATGTAGTCTTGTTACAGTTGACTTGTCCTCCAACAACATCACAAGCCCTCTTCCGGTTCGGTCTTTTCTTGAGGGTTGTGAACATCTGGCTTTGGTCAACCTCTCTGGCAATTCAATCCCAGGAGGGAGTCTCAGCTTGGGTGCTTCTTTGCTTCAGCTTGACATCTCTAGAAACCTGATTTCGGATTCATCCTTGTTGACTTGCCAGAATCTGAATCTGTTCAATGTTTCCGGTAACAAGCTGACCGGAAAACTGAGCGGTTCTATCTTGTCTTGCAAGAATCTGACAACACTTGACCTTTCATACAATGCTTTGTCTGGGGAGATACCAAACACTTTCCTAGAAAACGCCTCGGCATCTCTCAAGTATTTGGATCTCTCAAGCAACAATTTCACCGGAAAGTTTGCAAGCCTTGATTTCGGGCAGTGTAGCAGCCTCACTTTGCTGAAGCTATCACACAATAATCTCTATGGGGATGAGTTTCCTGCAAGCCTTGCAAACTGTCGAGCATTGGAGACACTAAACCTGACTAGTAATAAGTTGCAGGACAAAATTCCAGGTGCTTTGTTGGGAAATCTCAAGAAGTTGAGGCAACTCTTTCTGGGTCGCAATCAGTTTTCGGGTGAAATTCCAGCCGAATTAGGAAAGGCTTGTGGGACACTTCAGGAGCTTGATATTTCTGATAACATTCTTACTGGTGAATTGCCTTCAAGTTTTGTGTCATGCTCTTCTTTGGTTAGTCTCAATCTTGGTCGCAATCAGCTCTCAGGAAATTTCCTCAGTACTGTTGTGAGTAAGCTTCCGAGTTTGAGATATCTCTATGTACCCTTCAACAACATCACTGGTCCTGTGCCACCATCTATTACCAATGGTACTCGGCTTCAAGTGCTCGATCTTAGCGCTAATCTCTTCACAGGGAATGTTCCTTCAGTGTTTTGCTTCTCCAATGCTCCCTCAGCTTTGGAAAAGATACTTATAGCCAACAATTTTCTCTCCGGGACTGTGCCATCGGAACTTGGAAACTGCAAGAGCCTGAAGGCTATTGATCTGAGTTTCAACAATTTGAGTGGTGCAATTCCTTTAGAAGTTTGGACCTTGCCAAATCTTTCCGACTTGGTTATGTGGGCAAACAATCTCAGTGGTGAAATCCCGGAAGGCATTTGCATCAATGGAGGAAACCTGGAAACCCTGATTCTCAACAATAATCTCATTACTGGAGCCATTCCACAGTCCATTGGCAGATGCACCAATATGATTTGGGTGTCACTATCTAGCAACCGGCTTACAGGAGCAATCCCTCCAGGTATTGGAAATCTTCTTAATCTTGCTATACTCCAGTTGGGTAATAATTCACTGTCTGGTCAGATTCCAGCTGAGCTTGGCAAGTGTCAAAGCCTCATTTGGCTTGACCTGAACAGCAATGACCTAAATGGTTCTATCCCATCAGAGCTTGCTAACCAGGCCAGCCTAGTCAATCCTGGAATAGTTTCTGGGAAGCAGTTTGCATTTGTTAGAAATGAGGGTGGGACAGCTTGCAGGGGTGCAGGAGGACTAGTTGAGTTTGAGGGAGTCCGACCTCAGAGACTCGAAAATTTACCTATGGTTCATTCTTGTCCATCAACTAGAATTTACACAGGAATGACAGTTTACACATTCACCAGCAATGGAAGCATGATCTTCCTTGACATTTCCTACAATTACTTGTCGGGGACTATTCCCGCAAACTTAGGTACACTGTCCTATTTGCAAGTCTTCAATCTAGGACACAATATGTTAAGTGGAAATATTCCAGAAAGCTTTGGAGGATTAAAAGCAATTGGAGTCCTGGATCTCTCTCACAATAATCTTCAAGGTTATATCCCAGGATCTTTGGGGACTCTCTCTTTCCTGAGTGACCTTGATGTGTCTAACAACAACCTCACGGGCATTGTCCCTTCCGGTGGTCAGCTGACAACTTTCCCAGCATCCAGATATGAGAACAACTCTGGCCTTTGTGGGTTACCCCTGCCACCATGTGGCTCTCAAAGACATTCACCAGAGAGAGTTAGGGGAAAGAAGCCATCTATGGCTTCAGGAATGGTTATTGGCATCGCATTCTTTCTCTTCTGTATCCTTATACTTGCATTAGCTCTATATCGAGTGAAGAAGTACCAGCAGAAAGAGGCGAAGAGAGAAAATATATTGAAAGCCTTCCGACTTCAGGTAGCAGCAGCTGGAAACTTTCCAGTCATCAACGTAGCCACATTTGAAAAACCTTTGCGGAAGCTGACCTTCGCCCATTTACTTGAAGCCACCAATGGTTTCAGTGCTGATAGCTTGATTGGTTCTGGAGGGTTTGGTGAGGTCTACAAGGCACAATTGGGAGATGGCTGTGTTGTTGCAATCAAGAAGCTTATTCAAGTCACAGGTCAAGGAGACCGAGAGTTTATGGCAGAAATGGAAACTATTGGGAAAATCAAACACCGAAACCTGGTTCCTTTGCTGGGGTACTGCAAGGTTGGAGAGGAGAGACTTCTTGTGTATGAGTACATGAAATGGGGAAGTCTGGAGAGTGTTTTTCATGATAAGATTAAGGGAGGGGGCTCGAGGCTTGACTGGGCAGCACGAAAGAAGATTGCCATAGGGTCTGCTAGAGGTCTAGCATTCCTTCACCATAGCTGCATACCTCATATTATCCACCGGGACATGAAATCTAGCAATGTTCTTATAGATGAAAACTTTGAGGCCAGAGTATCGGATTTTGGCATGGCAAGATTGTGTGAGCACCCTAGCAGGAACTCCAGGTTACGTGCCCCCGAGTACTACCAGAGTTTTCGATGCACAACAAAAGGAGATGTCTACAGTTACGGTGTTATACTGCTAGAGCTTCTCTCAGGAAAAAGGCCTATAGACCCGTCAGCATTTGGTGATGACAACAACCTTGTTGGATGGGCAAAGCAGCTTCAAAGAGAAAAGAGATGGAATGAAATACTCGACACTGAGTTGATGACACAAAAATCTGGTGAGGCTGAACTATACCAGTATCTGAAGATTGCCTTTGAGTGCCTAGACGACAGGCCATTCCGGCGGCCAACAATGATTCAGGTTATGGCTATGTTTAAAGAGCTTCAGGTTGATTCGGAAAGTGATGTCCTGGATGGTTTTTCTCTCAAAGACACTGTTGCTGAGGAACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase

Protein Analysis

1177

Amino Acids

127.52

Weight (kDa)

6.53

Isoelectric Point (pI)

34.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 31 - 68 4.6e-08 Leucine rich repeat N-terminal domain
LRR_14 PF23598 282 - 371 2.5e-06 Leucine-rich repeat region
LRR_8 PF13855 339 - 399 8.9e-08 Leucine rich repeat
Island PF20141 609 - 672 1.8e-31 Brassinosteroid receptor island domain
LRR_8 PF13855 680 - 735 9.5e-08 Leucine rich repeat
LRR_8 PF13855 701 - 759 1.5e-06 Leucine rich repeat
Pkinase PF00069 889 - 1150 1.2e-37 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 890 - 1150 6.9e-40 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 1664, 2372
AccBSI CCGCTC 1 cut(s) 615
AccI GTMKAC 2 cut(s) 2703, 3195
AciI CCGC 4 cut(s) 615, 2076, 2617, 3424
AclWI GGATC 3 cut(s) 738, 2193, 2230
AcoI YGGCCR 1 cut(s) 3425
AcsI RAATTY 6 cut(s) 49, 909, 984, 1129, 1948, 1983
AdeI CACNNNGTG 1 cut(s) 2372
AfaI GTAC 9 cut(s) 302, 1141, 1182, 1231, 2089, 2507, 2846, 2885, 3158
AfeI AGCGCT 1 cut(s) 1258
AfiI CCNNNNNNNGG 8 cut(s) 409, 538, 1664, 2297, 2372, 2557, 2827, 2840
AhlI ACTAGT 2 cut(s) 887, 1909
AjiI CACGTC 1 cut(s) 214
Alw21I GWGCWC 2 cut(s) 1248, 3123
Alw26I GTCTC 7 cut(s) 248, 491, 866, 1103, 1933, 2766, 2860
AlwI GGATC 3 cut(s) 738, 2193, 2230
AlwNI CAGNNNCTG 3 cut(s) 568, 2570, 3382
Ama87I CYCGRG 2 cut(s) 2941, 3152
Aor51HI AGCGCT 1 cut(s) 1258
AoxI GGCC 8 cut(s) 190, 208, 1818, 2344, 3081, 3239, 3413, 3425
ApeKI GCWGC 7 cut(s) 62, 789, 2564, 2567, 2957, 3015, 3295
ApoI RAATTY 6 cut(s) 49, 909, 984, 1129, 1948, 1983
Asp700I GAANNNNTTC 3 cut(s) 575, 1843, 2546
AspLEI GCGC 1 cut(s) 1259
AspS9I GGNCC 2 cut(s) 1202, 1449
AsuC2I CCSGG 3 cut(s) 1354, 1508, 3038
AvaI CYCGRG 2 cut(s) 2941, 3152
AvaII GGWCC 2 cut(s) 1202, 1449
BaeGI GKGCMC 1 cut(s) 3151
BanII GRGCYC 1 cut(s) 2942
BbsI GAAGAC 3 cut(s) 248, 281, 2101
Bbv12I GWGCWC 2 cut(s) 1248, 3123
BbvCI CCTCAGC 2 cut(s) 1312, 3522
BbvI GCAGC 7 cut(s) 49, 801, 2576, 2579, 2969, 3002, 3307
BccI CCATC 9 cut(s) 1222, 1372, 1807, 1981, 2425, 2717, 3279, 3309, 3488
BciVI GTATCC 1 cut(s) 2477
BcnI CCSGG 3 cut(s) 1354, 1508, 3038
BcoDI GTCTC 7 cut(s) 248, 491, 866, 1103, 1933, 2766, 2860
BcuI ACTAGT 2 cut(s) 887, 1909
BfmI CTRYAG 2 cut(s) 3196, 3243
BfoI RGCGCY 1 cut(s) 1260
BfuI GTATCC 1 cut(s) 2477
BglI GCCNNNNNGGC 1 cut(s) 3421
BisI GCNGC 8 cut(s) 63, 790, 2565, 2568, 2958, 3016, 3296, 3425
BlpI GCTNAGC 1 cut(s) 1731
BlsI GCNGC 8 cut(s) 64, 791, 2566, 2569, 2959, 3017, 3297, 3426
BmcAI AGTACT 2 cut(s) 1141, 3158
Bme18I GGWCC 2 cut(s) 1202, 1449
BmeT110I CYCGRG 2 cut(s) 2941, 3152
BmgBI CACGTC 1 cut(s) 214
BmgT120I GGNCC 2 cut(s) 1202, 1449
BmiI GGNNCC 3 cut(s) 1576, 2832, 3528
BmrI ACTGGG 1 cut(s) 2962
BmsI GCATC 6 cut(s) 722, 1530, 1589, 2330, 2454, 3164
BmuI ACTGGG 1 cut(s) 2962
BpiI GAAGAC 3 cut(s) 248, 281, 2101
BplI GAGNNNNNCTC 6 cut(s) 471, 503, 2324, 2356, 3212, 3244
BpmI CTGGAG 6 cut(s) 1593, 1641, 1676, 2706, 2924, 3121
Bpu10I CCTNAGC 2 cut(s) 1312, 3522
Bpu1102I GCTNAGC 1 cut(s) 1731
BpuEI CTTGAG 5 cut(s) 117, 446, 704, 721, 920
BpuMI CCSGG 3 cut(s) 1354, 1508, 3038
BsaAI YACGTR 1 cut(s) 3146
BsaI GGTCTC 2 cut(s) 248, 2766
BsaJI CCNNGG 3 cut(s) 475, 708, 2218
BsaWI WCCGGW 5 cut(s) 409, 587, 602, 752, 2294
BsaXI ACNNNNNCTCC 4 cut(s) 368, 398, 1896, 1926
Bsc4I CCNNNNNNNGG 8 cut(s) 409, 538, 1664, 2297, 2372, 2557, 2827, 2840
Bse118I RCCGGY 1 cut(s) 1635
Bse1I ACTGG 8 cut(s) 155, 1057, 1204, 1576, 1693, 2582, 2957, 3376
Bse3DI GCAATG 3 cut(s) 1783, 2026, 3061
BseDI CCNNGG 3 cut(s) 475, 708, 2218
BseGI GGATG 6 cut(s) 82, 545, 838, 2321, 3290, 3499
BseLI CCNNNNNNNGG 8 cut(s) 409, 538, 1664, 2297, 2372, 2557, 2827, 2840
BseMI GCAATG 3 cut(s) 1783, 2026, 3061
BseNI ACTGG 8 cut(s) 155, 1057, 1204, 1576, 1693, 2582, 2957, 3376
BseRI GAGGAG 1 cut(s) 2876
BseSI GKGCMC 1 cut(s) 3151
BseXI GCAGC 7 cut(s) 49, 801, 2576, 2579, 2969, 3002, 3307
BseYI CCCAGC 2 cut(s) 2317, 2839
BsgI GTGCAG 1 cut(s) 1920
BshFI GGCC 8 cut(s) 192, 210, 1820, 2346, 3083, 3241, 3415, 3427
BsiHKAI GWGCWC 2 cut(s) 1248, 3123
BsiHKCI CYCGRG 2 cut(s) 2941, 3152
BslFI GGGAC 7 cut(s) 1027, 1369, 1897, 2080, 2245, 2273, 3053
BslI CCNNNNNNNGG 8 cut(s) 409, 538, 1664, 2297, 2372, 2557, 2827, 2840
BsmAI GTCTC 7 cut(s) 248, 491, 866, 1103, 1933, 2766, 2860
BsmFI GGGAC 7 cut(s) 1027, 1369, 1897, 2080, 2245, 2273, 3053
BsmI GAATGC 2 cut(s) 2450, 2999
BsnI GGCC 8 cut(s) 192, 210, 1820, 2346, 3083, 3241, 3415, 3427
Bso31I GGTCTC 2 cut(s) 248, 2766
BsoBI CYCGRG 2 cut(s) 2941, 3152
Bsp1286I GDGCHC 4 cut(s) 1248, 2942, 3123, 3151
Bsp143I GATC 7 cut(s) 331, 730, 1249, 1402, 2031, 2185, 2222
Bsp1720I GCTNAGC 1 cut(s) 1731
BspACI CCGC 4 cut(s) 615, 2076, 2617, 3424
BspANI GGCC 8 cut(s) 192, 210, 1820, 2346, 3083, 3241, 3415, 3427
BspHI TCATGA 1 cut(s) 2917
BspLI GGNNCC 3 cut(s) 1576, 2832, 3528
BspPI GGATC 3 cut(s) 738, 2193, 2230
BspQI GCTCTTC 1 cut(s) 1090
BspTNI GGTCTC 2 cut(s) 248, 2766
BsrBI CCGCTC 1 cut(s) 615
BsrDI GCAATG 3 cut(s) 1783, 2026, 3061
BsrFI RCCGGY 1 cut(s) 1635
BsrI ACTGG 8 cut(s) 155, 1057, 1204, 1576, 1693, 2582, 2957, 3376
BssAI RCCGGY 1 cut(s) 1635
BssECI CCNNGG 3 cut(s) 475, 708, 2218
BssMI GATC 7 cut(s) 331, 730, 1249, 1402, 2031, 2185, 2222
Bst6I CTCTTC 6 cut(s) 61, 411, 1090, 1271, 2465, 2519
BstAPI GCANNNNNTGC 1 cut(s) 851
BstBAI YACGTR 1 cut(s) 3146
BstC8I GCNNGC 5 cut(s) 766, 847, 1809, 1822, 1891
BstEII GGTNACC 1 cut(s) 2355
BstF5I GGATG 6 cut(s) 82, 545, 838, 2321, 3290, 3499
BstH2I RGCGCY 1 cut(s) 1260
BstHHI GCGC 1 cut(s) 1259
BstKTI GATC 7 cut(s) 334, 733, 1252, 1405, 2034, 2188, 2225
BstMAI GTCTC 7 cut(s) 248, 491, 866, 1103, 1933, 2766, 2860
BstMBI GATC 7 cut(s) 331, 730, 1249, 1402, 2031, 2185, 2222
BstPI GGTNACC 1 cut(s) 2355
BstSFI CTRYAG 2 cut(s) 3196, 3243
BstSLI GKGCMC 1 cut(s) 3151
BstV1I GCAGC 7 cut(s) 49, 801, 2576, 2579, 2969, 3002, 3307
BstV2I GAAGAC 3 cut(s) 248, 281, 2101
BstX2I RGATCY 3 cut(s) 730, 2185, 2222
BstYI RGATCY 3 cut(s) 730, 2185, 2222
BsuI GTATCC 1 cut(s) 2477
BsuRI GGCC 8 cut(s) 192, 210, 1820, 2346, 3083, 3241, 3415, 3427
BtgZI GCGATG 1 cut(s) 2431
BtrI CACGTC 1 cut(s) 214
BtsCI GGATG 6 cut(s) 82, 545, 838, 2321, 3290, 3499
BtsI GCAGTG 1 cut(s) 788
BtsIMutI CAGTG 9 cut(s) 788, 1197, 1293, 1501, 1709, 2090, 2668, 3333, 3513
Cac8I GCNNGC 5 cut(s) 766, 847, 1809, 1822, 1891
CaiI CAGNNNCTG 3 cut(s) 568, 2570, 3382
CciI TCATGA 1 cut(s) 2917
CfoI GCGC 1 cut(s) 1259
Cfr10I RCCGGY 1 cut(s) 1635
Cfr13I GGNCC 2 cut(s) 1202, 1449
CsiI ACCWGGT 1 cut(s) 2826
Csp6I GTAC 9 cut(s) 301, 1140, 1181, 1230, 2088, 2506, 2845, 2884, 3157
CspCI CAANNNNNGTGG 4 cut(s) 1405, 1440, 1477, 1512
CviQI GTAC 9 cut(s) 301, 1140, 1181, 1230, 2088, 2506, 2845, 2884, 3157
DpnI GATC 7 cut(s) 333, 732, 1251, 1404, 2033, 2187, 2224
DpnII GATC 7 cut(s) 331, 730, 1249, 1402, 2031, 2185, 2222
DraI TTTAAA 1 cut(s) 3457
DraIII CACNNNGTG 1 cut(s) 2372
EaeI YGGCCR 1 cut(s) 3425
Eam1104I CTCTTC 6 cut(s) 61, 411, 1090, 1271, 2465, 2519
EarI CTCTTC 6 cut(s) 61, 411, 1090, 1271, 2465, 2519
Eco147I AGGCCT 2 cut(s) 192, 3241
Eco24I GRGCYC 1 cut(s) 2942
Eco31I GGTCTC 2 cut(s) 248, 2766
Eco32I GATATC 1 cut(s) 1172
Eco47I GGWCC 2 cut(s) 1202, 1449
Eco47III AGCGCT 1 cut(s) 1258
Eco88I CYCGRG 2 cut(s) 2941, 3152
Eco91I GGTNACC 1 cut(s) 2355
EcoO65I GGTNACC 1 cut(s) 2355
EcoRV GATATC 1 cut(s) 1172
EcoT38I GRGCYC 1 cut(s) 2942
FalI AAGNNNNNCTT 4 cut(s) 2612, 2644, 3377, 3409
FaqI GGGAC 7 cut(s) 1027, 1369, 1897, 2080, 2245, 2273, 3053
FauI CCCGC 1 cut(s) 2083
FblI GTMKAC 2 cut(s) 2703, 3195
Fnu4HI GCNGC 8 cut(s) 63, 790, 2565, 2568, 2958, 3016, 3296, 3425
FokI GGATG 6 cut(s) 89, 532, 845, 2308, 3297, 3506
FriOI GRGCYC 1 cut(s) 2942
Fsp4HI GCNGC 8 cut(s) 63, 790, 2565, 2568, 2958, 3016, 3296, 3425
GlaI GCGC 1 cut(s) 1258
GluI GCNGC 8 cut(s) 63, 790, 2565, 2568, 2958, 3016, 3296, 3425
GsaI CCCAGC 2 cut(s) 2321, 2843
GsuI CTGGAG 6 cut(s) 1593, 1641, 1676, 2706, 2924, 3121
HaeII RGCGCY 1 cut(s) 1260
HaeIII GGCC 8 cut(s) 192, 210, 1820, 2346, 3083, 3241, 3415, 3427
HhaI GCGC 1 cut(s) 1259
Hin6I GCGC 1 cut(s) 1257
HinP1I GCGC 1 cut(s) 1257
HincII GTYRAC 3 cut(s) 376, 454, 555
HindII GTYRAC 3 cut(s) 376, 454, 555
HindIII AAGCTT 4 cut(s) 89, 1154, 2152, 2747
Hpy166II GTNNAC 8 cut(s) 376, 437, 454, 555, 2005, 2090, 2704, 3196
Hpy8I GTNNAC 8 cut(s) 376, 437, 454, 555, 2005, 2090, 2704, 3196
HpyCH4IV ACGT 3 cut(s) 213, 2592, 3145
HpySE526I ACGT 3 cut(s) 213, 2592, 3145
HspAI GCGC 1 cut(s) 1257
Kzo9I GATC 7 cut(s) 331, 730, 1249, 1402, 2031, 2185, 2222
LguI GCTCTTC 1 cut(s) 1090
LmnI GCTCC 4 cut(s) 1024, 1312, 1574, 1646
Lsp1109I GCAGC 7 cut(s) 49, 801, 2576, 2579, 2969, 3002, 3307
LweI GCATC 6 cut(s) 722, 1530, 1589, 2330, 2454, 3164
MabI ACCWGGT 1 cut(s) 2826
MaeII ACGT 3 cut(s) 213, 2592, 3145
MaeIII GTNAC 8 cut(s) 367, 590, 1620, 2249, 2355, 2758, 3141, 3200
MalI GATC 7 cut(s) 333, 732, 1251, 1404, 2033, 2187, 2224
MbiI CCGCTC 1 cut(s) 615
MboI GATC 7 cut(s) 331, 730, 1249, 1402, 2031, 2185, 2222
MfeI CAATTG 2 cut(s) 2172, 2714
MflI RGATCY 3 cut(s) 730, 2185, 2222
MhlI GDGCHC 4 cut(s) 1248, 2942, 3123, 3151
MlyI GAGTC 6 cut(s) 295, 493, 1935, 1935, 2187, 2227
MmeI TCCRAC 8 cut(s) 290, 369, 411, 1491, 1954, 2575, 2836, 3262
MroXI GAANNNNTTC 3 cut(s) 575, 1843, 2546
MseI TTAA 5 cut(s) 1677, 2132, 2165, 2928, 3456
MslI CAYNNNNRTG 2 cut(s) 1607, 1994
MspA1I CMGCKG 4 cut(s) 62, 1730, 2305, 2570
MunI CAATTG 2 cut(s) 2172, 2714
Mva1269I GAATGC 2 cut(s) 2450, 2999
NciI CCSGG 3 cut(s) 1354, 1508, 3038
NdeII GATC 7 cut(s) 331, 730, 1249, 1402, 2031, 2185, 2222
NlaIV GGNNCC 3 cut(s) 1576, 2832, 3528
NmeAIII GCCGAG 3 cut(s) 309, 689, 1213
NmuCI GTSAC 3 cut(s) 1620, 2249, 2758
PaeR7I CTCGAG 1 cut(s) 2941
PagI TCATGA 1 cut(s) 2917
PceI AGGCCT 2 cut(s) 192, 3241
PciSI GCTCTTC 1 cut(s) 1090
PctI GAATGC 2 cut(s) 2450, 2999
PdmI GAANNNNTTC 3 cut(s) 575, 1843, 2546
PfeI GAWTC 9 cut(s) 161, 542, 565, 571, 637, 1549, 1723, 3436, 3473
PflMI CCANNNNNTGG 2 cut(s) 1664, 2372
PfoI TCCNGGA 5 cut(s) 1352, 1506, 1834, 2181, 3489
PkrI GCNGC 8 cut(s) 64, 791, 2566, 2569, 2959, 3017, 3297, 3426
PleI GAGTC 6 cut(s) 294, 492, 1934, 1935, 2186, 2227
PpsI GAGTC 6 cut(s) 294, 492, 1934, 1935, 2186, 2227
Ppu21I YACGTR 1 cut(s) 3146
PspEI GGTNACC 1 cut(s) 2355
PspFI CCCAGC 2 cut(s) 2317, 2839
PspN4I GGNNCC 3 cut(s) 1576, 2832, 3528
PspPI GGNCC 2 cut(s) 1202, 1449
PspXI VCTCGAGB 1 cut(s) 2941
PstNI CAGNNNCTG 3 cut(s) 568, 2570, 3382
PsuI RGATCY 3 cut(s) 730, 2185, 2222
PvuII CAGCTG 4 cut(s) 62, 1730, 2305, 2570
RsaI GTAC 9 cut(s) 302, 1141, 1182, 1231, 2089, 2507, 2846, 2885, 3158
RsaNI GTAC 9 cut(s) 301, 1140, 1181, 1230, 2088, 2506, 2845, 2884, 3157
RseI CAYNNNNRTG 2 cut(s) 1607, 1994
SapI GCTCTTC 1 cut(s) 1090
SaqAI TTAA 5 cut(s) 1677, 2132, 2165, 2928, 3456
SatI GCNGC 8 cut(s) 63, 790, 2565, 2568, 2958, 3016, 3296, 3425
Sau3AI GATC 7 cut(s) 331, 730, 1249, 1402, 2031, 2185, 2222
Sau96I GGNCC 2 cut(s) 1202, 1449
ScaI AGTACT 2 cut(s) 1141, 3158
SchI GAGTC 6 cut(s) 295, 493, 1935, 1935, 2187, 2227
SduI GDGCHC 4 cut(s) 1248, 2942, 3123, 3151
SexAI ACCWGGT 1 cut(s) 2826
SfaNI GCATC 6 cut(s) 722, 1530, 1589, 2330, 2454, 3164
SfcI CTRYAG 2 cut(s) 3196, 3243
Sfr274I CTCGAG 1 cut(s) 2941
SinI GGWCC 2 cut(s) 1202, 1449
SlaI CTCGAG 1 cut(s) 2941
SmiMI CAYNNNNRTG 2 cut(s) 1607, 1994
SmlI CTYRAG 6 cut(s) 132, 425, 719, 736, 935, 2941
SmoI CTYRAG 6 cut(s) 132, 425, 719, 736, 935, 2941
SpeI ACTAGT 2 cut(s) 887, 1909
SseBI AGGCCT 2 cut(s) 192, 3241
SsiI CCGC 4 cut(s) 615, 2076, 2617, 3424
SspI AATATT 1 cut(s) 2143
StuI AGGCCT 2 cut(s) 192, 3241
TaiI ACGT 3 cut(s) 216, 2595, 3148
TaqI TCGA 8 cut(s) 225, 862, 1248, 1944, 2494, 2942, 3172, 3330
TaqII GACCGA 2 cut(s) 405, 2790
TatI WGTACW 4 cut(s) 300, 1139, 2883, 3156
TauI GCSGC 1 cut(s) 3427
TfiI GAWTC 9 cut(s) 161, 542, 565, 571, 637, 1549, 1723, 3436, 3473
Tru1I TTAA 5 cut(s) 1677, 2132, 2165, 2928, 3456
Tru9I TTAA 5 cut(s) 1677, 2132, 2165, 2928, 3456
TscAI CASTG 9 cut(s) 788, 1204, 1293, 1501, 1716, 2097, 2668, 3340, 3520
TseFI GTSAC 3 cut(s) 1620, 2249, 2758
TseI GCWGC 7 cut(s) 62, 789, 2564, 2567, 2957, 3015, 3295
Tsp45I GTSAC 3 cut(s) 1620, 2249, 2758
TspRI CASTG 9 cut(s) 788, 1204, 1293, 1501, 1716, 2097, 2668, 3340, 3520
Van91I CCANNNNNTGG 2 cut(s) 1664, 2372
VpaK11BI GGWCC 2 cut(s) 1202, 1449
XapI RAATTY 6 cut(s) 49, 909, 984, 1129, 1948, 1983
XbaI TCTAGA 1 cut(s) 524
XhoI CTCGAG 1 cut(s) 2941
XmiI GTMKAC 2 cut(s) 2703, 3195
XmnI GAANNNNTTC 3 cut(s) 575, 1843, 2546
ZrmI AGTACT 2 cut(s) 1141, 3158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.