MD03G1060600.v1.1

Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003525
Physical Location & Seq
Forward (+)
4896792 .. 4898613
1822 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1060600.v1.1.491

Sequence Viewer

Length: 390 bp
ATGCTTCTCTTGCTCGCCAAAAATATTCTGACAAATCAGAAGCTGCCATCAATGAACAGATCAAGTGTATTTTTCAAAGAATCAAGTGAAGTAGAAAGGCAGCATGCTGAAAAACTTATGGAATACCAGAACAAACGCGGAGGGAAAGTTAAGCTACAGTCCATGCGAATGCCCGTCTCCGAGTTTGGTCATGTCAAAAAGGGTGATGCATTGTGTGCAAACGAAAACAATGATGTGCAACTGGTGGATTTTGTGGAGAGTAACTATTTGACTGAGCAGGTGGAAGCCATATATAAAATGTCCAAGTATGTAGCTCAATTGAGAAGGATCGTCACTGGACATGGTGTTCGGCACTTTGATCAGATGCTCCTCAATGGCAATGCTGCATGA

Protein Analysis

130

Amino Acids

14.76

Weight (kDa)

9.27

Isoelectric Point (pI)

48.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ferritin PF00210 16 - 63 5.1e-07 Ferritin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 268
Acc36I ACCTGC 1 cut(s) 268
AccII CGCG 1 cut(s) 138
AciI CCGC 1 cut(s) 138
AclWI GGATC 1 cut(s) 335
AgsI TTSAA 1 cut(s) 76
AloI GAACNNNNNNTCC 2 cut(s) 330, 362
AluBI AGCT 3 cut(s) 43, 154, 314
AluI AGCT 3 cut(s) 43, 154, 314
Alw26I GTCTC 1 cut(s) 181
AlwI GGATC 1 cut(s) 335
AlwNI CAGNNNCTG 1 cut(s) 43
ApeKI GCWGC 3 cut(s) 43, 100, 383
ArsI GACNNNNNNTTYG 2 cut(s) 330, 362
AsuHPI GGTGA 1 cut(s) 215
BbvI GCAGC 3 cut(s) 30, 112, 370
BccI CCATC 1 cut(s) 55
BclI TGATCA 1 cut(s) 358
BcoDI GTCTC 1 cut(s) 181
BfmI CTRYAG 1 cut(s) 155
BfuAI ACCTGC 1 cut(s) 268
BisI GCNGC 3 cut(s) 44, 101, 384
BlsI GCNGC 3 cut(s) 45, 102, 385
BmsI GCATC 2 cut(s) 196, 354
BsaXI ACNNNNNCTCC 2 cut(s) 132, 162
Bse1I ACTGG 2 cut(s) 246, 340
Bse3DI GCAATG 1 cut(s) 385
BseMI GCAATG 1 cut(s) 385
BseMII CTCAG 1 cut(s) 264
BseNI ACTGG 2 cut(s) 246, 340
BseRI GAGGAG 1 cut(s) 359
BseXI GCAGC 3 cut(s) 30, 112, 370
Bsh1236I CGCG 1 cut(s) 138
BsmAI GTCTC 1 cut(s) 181
BsmBI CGTCTC 1 cut(s) 181
BsmI GAATGC 1 cut(s) 174
Bsp143I GATC 3 cut(s) 59, 327, 358
BspACI CCGC 1 cut(s) 138
BspCNI CTCAG 1 cut(s) 265
BspFNI CGCG 1 cut(s) 138
BspMI ACCTGC 1 cut(s) 268
BspPI GGATC 1 cut(s) 335
BsrDI GCAATG 1 cut(s) 385
BsrI ACTGG 2 cut(s) 246, 340
BssMI GATC 3 cut(s) 59, 327, 358
Bst4CI ACNGT 1 cut(s) 159
BstAPI GCANNNNNTGC 1 cut(s) 215
BstC8I GCNNGC 2 cut(s) 15, 105
BstDEI CTNAG 1 cut(s) 273
BstFNI CGCG 1 cut(s) 138
BstKTI GATC 3 cut(s) 62, 330, 361
BstMAI GTCTC 1 cut(s) 181
BstMBI GATC 3 cut(s) 59, 327, 358
BstMWI GCNNNNNNNGC 2 cut(s) 10, 215
BstNSI RCATGY 1 cut(s) 107
BstSFI CTRYAG 1 cut(s) 155
BstUI CGCG 1 cut(s) 138
BstV1I GCAGC 3 cut(s) 30, 112, 370
BtsIMutI CAGTG 1 cut(s) 333
BveI ACCTGC 1 cut(s) 268
Cac8I GCNNGC 2 cut(s) 15, 105
CaiI CAGNNNCTG 1 cut(s) 43
CviAII CATG 5 cut(s) 104, 163, 191, 341, 387
CviJI RGCY 4 cut(s) 43, 154, 287, 314
CviKI_1 RGCY 4 cut(s) 43, 154, 287, 314
DdeI CTNAG 1 cut(s) 273
DpnI GATC 3 cut(s) 61, 329, 360
DpnII GATC 3 cut(s) 59, 327, 358
EcoT22I ATGCAT 1 cut(s) 211
Esp3I CGTCTC 1 cut(s) 181
FaeI CATG 5 cut(s) 107, 166, 194, 344, 390
FatI CATG 5 cut(s) 103, 162, 190, 340, 386
FbaI TGATCA 1 cut(s) 358
Fnu4HI GCNGC 3 cut(s) 44, 101, 384
Fsp4HI GCNGC 3 cut(s) 44, 101, 384
GluI GCNGC 3 cut(s) 44, 101, 384
Hin1II CATG 5 cut(s) 107, 166, 194, 344, 390
HinfI GANTC 1 cut(s) 80
HphI GGTGA 1 cut(s) 215
Hpy188I TCNGA 4 cut(s) 30, 39, 181, 363
HpyAV CCTTC 1 cut(s) 318
HpyCH4III ACNGT 1 cut(s) 159
HpyCH4V TGCA 4 cut(s) 209, 218, 238, 386
HpyF10VI GCNNNNNNNGC 2 cut(s) 10, 215
HpyF3I CTNAG 1 cut(s) 273
Hsp92II CATG 5 cut(s) 107, 166, 194, 344, 390
Ksp22I TGATCA 1 cut(s) 358
Kzo9I GATC 3 cut(s) 59, 327, 358
LmnI GCTCC 1 cut(s) 372
LpnPI CCDG 4 cut(s) 140, 227, 263, 321
Lsp1109I GCAGC 3 cut(s) 30, 112, 370
LweI GCATC 2 cut(s) 196, 354
MaeIII GTNAC 2 cut(s) 260, 331
MalI GATC 3 cut(s) 61, 329, 360
MboI GATC 3 cut(s) 59, 327, 358
MfeI CAATTG 1 cut(s) 317
MluCI AATT 1 cut(s) 317
MnlI CCTC 2 cut(s) 134, 380
Mph1103I ATGCAT 1 cut(s) 211
MseI TTAA 1 cut(s) 150
MslI CAYNNNNRTG 1 cut(s) 167
MunI CAATTG 1 cut(s) 317
Mva1269I GAATGC 1 cut(s) 174
MvnI CGCG 1 cut(s) 138
MwoI GCNNNNNNNGC 2 cut(s) 10, 215
NdeII GATC 3 cut(s) 59, 327, 358
NlaIII CATG 5 cut(s) 107, 166, 194, 344, 390
NmuCI GTSAC 1 cut(s) 331
NsiI ATGCAT 1 cut(s) 211
NspI RCATGY 1 cut(s) 107
PaeI GCATGC 1 cut(s) 107
PaqCI CACCTGC 1 cut(s) 268
PctI GAATGC 1 cut(s) 174
PfeI GAWTC 1 cut(s) 80
PkrI GCNGC 3 cut(s) 45, 102, 385
PstNI CAGNNNCTG 1 cut(s) 43
RseI CAYNNNNRTG 1 cut(s) 167
SaqAI TTAA 1 cut(s) 150
SatI GCNGC 3 cut(s) 44, 101, 384
Sau3AI GATC 3 cut(s) 59, 327, 358
SetI ASST 4 cut(s) 45, 156, 282, 316
SfaNI GCATC 2 cut(s) 196, 354
SfcI CTRYAG 1 cut(s) 155
SmiMI CAYNNNNRTG 1 cut(s) 167
SphI GCATGC 1 cut(s) 107
Sse9I AATT 1 cut(s) 317
SsiI CCGC 1 cut(s) 138
SspI AATATT 1 cut(s) 25
TaaI ACNGT 1 cut(s) 159
TasI AATT 1 cut(s) 317
TfiI GAWTC 1 cut(s) 80
Tru1I TTAA 1 cut(s) 150
Tru9I TTAA 1 cut(s) 150
TscAI CASTG 1 cut(s) 340
TseFI GTSAC 1 cut(s) 331
TseI GCWGC 3 cut(s) 43, 100, 383
Tsp45I GTSAC 1 cut(s) 331
TspDTI ATGAA 1 cut(s) 68
TspRI CASTG 1 cut(s) 340
XceI RCATGY 1 cut(s) 107
Zsp2I ATGCAT 1 cut(s) 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.