MD12G1178200.v1.1

Ferritin-4, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0003525
Physical Location & Seq
Reverse (-)
25882856 .. 25885061
2206 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1178200.v1.1.491

Sequence Viewer

Length: 912 bp
ATGCCAACTGGATTTCCTCCATATCCCTATATAGCCCCTCACCCCACTCTCAGCCTCCACATTTCTTCACCGTTCTCCATTTCCTCTGCAATTTCCAGTCCAGATTCTTGTTCAAACTCTCTTCTTTCCAATCACCCGTTCGAGATAATCCGATCGAAAACCGTGTCGATGATGTCTCTGAGGGCCATTTCGACGTTTTCCGTCCCATCCAAGCTAGGGGACAAAGGCGGTGCTGTTACCACCCTCTTGCCCAACTCAAAACTTGGATCATCCTCTTCTACTCTGAGCTTTAAACCGCAGCGAAAACTCGAAAAGTTTGCGGCTTCTGTATCGAGCGAAGCAGTGGCGTTGACCGGCGTGGTGTTTCAGTCTCTCTTGCCCGTCAGCGTTACACAGATGAGTCCGAGGCCGCCATTAACGAGCAGATCAAGTGTTGTGGAATACAATGTGTCGTATGTGTACCATGCACTGTTTGCGTACTTTGACAGGGACAACGTTGCTCTCAAGGGCCTTGCCAACTTTTTCAAGGAATCGAGCGAGGAAGAAAGAGAACATGCCGAAAAGCTGATGGAATATCAGAACAAGCGAGGTGGGAGAGTGAAACTGCACTCTGTAATCGCGGCGCCTACGGAGTTTGATCATGCAGAAAAGGGAGATGCATTGTATGCTATGGAGTTAGCATTGTCTTTGGAGAAGCTGACAAATGAAAAACTCTTGAACTTGCATAAGGTTGCAGACCAGAACAATGATCCCCAATTGATGGATTTTATCGAGAGCGAGTTTCTGGCTGAGCAGGTTGAGGCCATCAAGAAGATAGCTGACTATGTCACTCAACTGAGAAGGGTTGGAAAAGGCCATGGAGTGTGGCACTTTGACCAGTATCTCCTTCATGAGGGTGATGCTGCAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000041 GO:0000302 GO:0003006 GO:0003674 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006810 GO:0006811 GO:0006812 GO:0006826 GO:0006873 GO:0006875 GO:0006879 GO:0006880 GO:0006950 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009507 GO:0009532 GO:0009534 GO:0009535 GO:0009536 GO:0009570 GO:0009579 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009636 GO:0009719 GO:0009725 GO:0009735 GO:0009791 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010043 GO:0015979 GO:0016020 GO:0019725 GO:0019904 GO:0022414 GO:0030001 GO:0030003 GO:0031976 GO:0031984 GO:0032501 GO:0032502 GO:0034357 GO:0042221 GO:0042493 GO:0042542 GO:0042592 GO:0042651 GO:0043167 GO:0043169 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0046677 GO:0046872 GO:0046914 GO:0046916 GO:0048366 GO:0048367 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0048878 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051235 GO:0051238 GO:0051641 GO:0051651 GO:0051704 GO:0051707 GO:0055035 GO:0055065 GO:0055072 GO:0055076 GO:0055080 GO:0055082 GO:0061458 GO:0065007 GO:0065008 GO:0090567 GO:0097577 GO:0098771 GO:0099402 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko04216 ko04217 ko04978 map04216 map04217 map04978
Pfam Domains
Protein Families
Ferritin

Protein Analysis

304

Amino Acids

33.44

Weight (kDa)

6.42

Isoelectric Point (pI)

48.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ferritin PF00210 147 - 282 1.4e-31 Ferritin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 784
AccB1I GGYRCC 1 cut(s) 622
AccB7I CCANNNNNTGG 1 cut(s) 760
AccII CGCG 1 cut(s) 620
AciI CCGC 5 cut(s) 228, 296, 320, 410, 620
AclI AACGTT 1 cut(s) 495
AclWI GGATC 2 cut(s) 274, 743
AcyI GRCGYC 1 cut(s) 623
AfaI GTAC 2 cut(s) 461, 479
AfiI CCNNNNNNNGG 3 cut(s) 216, 760, 892
AgsI TTSAA 3 cut(s) 114, 526, 718
AjuI GAANNNNNNNTTGG 2 cut(s) 122, 154
AluBI AGCT 5 cut(s) 214, 288, 565, 697, 818
AluI AGCT 5 cut(s) 214, 288, 565, 697, 818
Alw26I GTCTC 2 cut(s) 180, 375
AlwI GGATC 2 cut(s) 274, 743
AoxI GGCC 5 cut(s) 183, 407, 508, 801, 853
ApeKI GCWGC 2 cut(s) 298, 902
ArsI GACNNNNNNTTYG 2 cut(s) 149, 181
AspLEI GCGC 1 cut(s) 625
AspS9I GGNCC 2 cut(s) 183, 508
AsuHPI GGTGA 4 cut(s) 32, 60, 125, 908
BanI GGYRCC 1 cut(s) 622
BbvI GCAGC 2 cut(s) 310, 889
BccI CCATC 4 cut(s) 214, 562, 754, 812
BcgI CGANNNNNNTGC 2 cut(s) 299, 333
BclI TGATCA 1 cut(s) 637
BcoDI GTCTC 2 cut(s) 180, 375
BfaI CTAG 1 cut(s) 215
BfoI RGCGCY 1 cut(s) 626
BfuAI ACCTGC 1 cut(s) 784
BisI GCNGC 5 cut(s) 299, 321, 410, 621, 903
BlpI GCTNAGC 1 cut(s) 789
BlsI GCNGC 5 cut(s) 300, 322, 411, 622, 904
BmgT120I GGNCC 2 cut(s) 183, 508
BmiI GGNNCC 1 cut(s) 624
BmsI GCATC 2 cut(s) 646, 889
Bpu1102I GCTNAGC 1 cut(s) 789
BpuEI CTTGAG 1 cut(s) 488
BsaHI GRCGYC 1 cut(s) 623
BsaJI CCNNGG 2 cut(s) 404, 856
Bsc4I CCNNNNNNNGG 3 cut(s) 216, 760, 892
Bse118I RCCGGY 1 cut(s) 353
Bse1I ACTGG 3 cut(s) 13, 96, 877
BseDI CCNNGG 2 cut(s) 404, 856
BseGI GGATG 2 cut(s) 206, 269
BseLI CCNNNNNNNGG 3 cut(s) 216, 760, 892
BseMII CTCAG 5 cut(s) 64, 170, 275, 780, 827
BseNI ACTGG 3 cut(s) 13, 96, 877
BseXI GCAGC 2 cut(s) 310, 889
BsgI GTGCAG 1 cut(s) 590
Bsh1236I CGCG 1 cut(s) 620
Bsh1285I CGRYCG 1 cut(s) 155
BshFI GGCC 5 cut(s) 185, 409, 510, 803, 855
BshNI GGYRCC 1 cut(s) 622
BsiEI CGRYCG 1 cut(s) 155
BsiSI CCGG 1 cut(s) 354
BslFI GGGAC 3 cut(s) 188, 233, 503
BslI CCNNNNNNNGG 3 cut(s) 216, 760, 892
BsmAI GTCTC 2 cut(s) 180, 375
BsmFI GGGAC 3 cut(s) 188, 233, 503
BsnI GGCC 5 cut(s) 185, 409, 510, 803, 855
Bsp143I GATC 5 cut(s) 152, 266, 425, 637, 748
Bsp1720I GCTNAGC 1 cut(s) 789
Bsp19I CCATGG 1 cut(s) 856
BspACI CCGC 5 cut(s) 228, 296, 320, 410, 620
BspANI GGCC 5 cut(s) 185, 409, 510, 803, 855
BspCNI CTCAG 5 cut(s) 63, 171, 276, 781, 828
BspFNI CGCG 1 cut(s) 620
BspHI TCATGA 1 cut(s) 889
BspLI GGNNCC 1 cut(s) 624
BspMI ACCTGC 1 cut(s) 784
BspPI GGATC 2 cut(s) 274, 743
BspT107I GGYRCC 1 cut(s) 622
BsrFI RCCGGY 1 cut(s) 353
BsrI ACTGG 3 cut(s) 13, 96, 877
BssAI RCCGGY 1 cut(s) 353
BssECI CCNNGG 2 cut(s) 404, 856
BssMI GATC 5 cut(s) 152, 266, 425, 637, 748
BssNI GRCGYC 1 cut(s) 623
BssT1I CCWWGG 1 cut(s) 856
Bst4CI ACNGT 3 cut(s) 72, 163, 471
Bst6I CTCTTC 2 cut(s) 126, 280
BstACI GRCGYC 1 cut(s) 623
BstAPI GCANNNNNTGC 2 cut(s) 473, 665
BstDEI CTNAG 5 cut(s) 50, 179, 284, 789, 836
BstDSI CCRYGG 1 cut(s) 856
BstENI CCTNNNNNAGG 1 cut(s) 890
BstF5I GGATG 2 cut(s) 206, 269
BstFNI CGCG 1 cut(s) 620
BstH2I RGCGCY 1 cut(s) 626
BstHHI GCGC 1 cut(s) 625
BstKTI GATC 5 cut(s) 155, 269, 428, 640, 751
BstMAI GTCTC 2 cut(s) 180, 375
BstMBI GATC 5 cut(s) 152, 266, 425, 637, 748
BstMCI CGRYCG 1 cut(s) 155
BstMWI GCNNNNNNNGC 2 cut(s) 473, 665
BstNSI RCATGY 1 cut(s) 557
BstUI CGCG 1 cut(s) 620
BstV1I GCAGC 2 cut(s) 310, 889
BsuRI GGCC 5 cut(s) 185, 409, 510, 803, 855
BtgI CCRYGG 1 cut(s) 856
BtsCI GGATG 2 cut(s) 206, 269
BtsI GCAGTG 1 cut(s) 348
BtsIMutI CAGTG 2 cut(s) 348, 467
BveI ACCTGC 1 cut(s) 784
CciI TCATGA 1 cut(s) 889
CfoI GCGC 1 cut(s) 625
Cfr10I RCCGGY 1 cut(s) 353
Cfr13I GGNCC 2 cut(s) 183, 508
Csp6I GTAC 2 cut(s) 460, 478
CspCI CAANNNNNGTGG 4 cut(s) 417, 452, 571, 606
CviAII CATG 5 cut(s) 464, 554, 641, 857, 890
CviQI GTAC 2 cut(s) 460, 478
DdeI CTNAG 5 cut(s) 50, 179, 284, 789, 836
DinI GGCGCC 1 cut(s) 624
DpnI GATC 5 cut(s) 154, 268, 427, 639, 750
DpnII GATC 5 cut(s) 152, 266, 425, 637, 748
DraI TTTAAA 1 cut(s) 292
Eam1104I CTCTTC 2 cut(s) 126, 280
EarI CTCTTC 2 cut(s) 126, 280
Eco130I CCWWGG 1 cut(s) 856
EcoNI CCTNNNNNAGG 1 cut(s) 890
EcoO109I RGGNCCY 1 cut(s) 508
EcoT14I CCWWGG 1 cut(s) 856
EcoT22I ATGCAT 1 cut(s) 661
EgeI GGCGCC 1 cut(s) 624
EheI GGCGCC 1 cut(s) 624
ErhI CCWWGG 1 cut(s) 856
FaeI CATG 5 cut(s) 467, 557, 644, 860, 893
FaqI GGGAC 3 cut(s) 188, 233, 503
FatI CATG 5 cut(s) 463, 553, 640, 856, 889
FbaI TGATCA 1 cut(s) 637
Fnu4HI GCNGC 5 cut(s) 299, 321, 410, 621, 903
FokI GGATG 2 cut(s) 193, 256
Fsp4HI GCNGC 5 cut(s) 299, 321, 410, 621, 903
FspBI CTAG 1 cut(s) 215
GlaI GCGC 1 cut(s) 624
GluI GCNGC 5 cut(s) 299, 321, 410, 621, 903
HaeII RGCGCY 1 cut(s) 626
HaeIII GGCC 5 cut(s) 185, 409, 510, 803, 855
HapII CCGG 1 cut(s) 354
HhaI GCGC 1 cut(s) 625
Hin1I GRCGYC 1 cut(s) 623
Hin1II CATG 5 cut(s) 467, 557, 644, 860, 893
Hin6I GCGC 1 cut(s) 623
HinP1I GCGC 1 cut(s) 623
HincII GTYRAC 1 cut(s) 351
HindII GTYRAC 1 cut(s) 351
HinfI GANTC 3 cut(s) 104, 400, 530
HpaII CCGG 1 cut(s) 354
HphI GGTGA 4 cut(s) 32, 60, 125, 908
Hpy166II GTNNAC 2 cut(s) 351, 460
Hpy188I TCNGA 5 cut(s) 152, 180, 285, 405, 579
Hpy188III TCNNGA 6 cut(s) 101, 142, 715, 772, 808, 890
Hpy8I GTNNAC 2 cut(s) 351, 460
Hpy99I CGWCG 1 cut(s) 196
HpyAV CCTTC 2 cut(s) 834, 896
HpyCH4III ACNGT 3 cut(s) 72, 163, 471
HpyCH4IV ACGT 2 cut(s) 194, 495
HpyCH4V TGCA 8 cut(s) 89, 467, 607, 644, 659, 724, 734, 905
HpyF10VI GCNNNNNNNGC 2 cut(s) 473, 665
HpyF3I CTNAG 5 cut(s) 50, 179, 284, 789, 836
HpySE526I ACGT 2 cut(s) 194, 495
Hsp92I GRCGYC 1 cut(s) 623
Hsp92II CATG 5 cut(s) 467, 557, 644, 860, 893
HspAI GCGC 1 cut(s) 623
KasI GGCGCC 1 cut(s) 622
Ksp22I TGATCA 1 cut(s) 637
Kzo9I GATC 5 cut(s) 152, 266, 425, 637, 748
LpnPI CCDG 8 cut(s) 109, 114, 367, 472, 752, 770, 779, 890
Lsp1109I GCAGC 2 cut(s) 310, 889
LweI GCATC 2 cut(s) 646, 889
MaeI CTAG 1 cut(s) 215
MaeII ACGT 2 cut(s) 194, 495
MaeIII GTNAC 3 cut(s) 235, 388, 826
MalI GATC 5 cut(s) 154, 268, 427, 639, 750
MboI GATC 5 cut(s) 152, 266, 425, 637, 748
MboII GAAGA 5 cut(s) 57, 113, 267, 554, 823
MfeI CAATTG 1 cut(s) 755
MluCI AATT 2 cut(s) 90, 755
Mly113I GGCGCC 1 cut(s) 623
MlyI GAGTC 1 cut(s) 409
MmeI TCCRAC 1 cut(s) 826
Mph1103I ATGCAT 1 cut(s) 661
MseI TTAA 2 cut(s) 291, 416
MslI CAYNNNNRTG 1 cut(s) 894
MspI CCGG 1 cut(s) 354
MunI CAATTG 1 cut(s) 755
MvnI CGCG 1 cut(s) 620
MwoI GCNNNNNNNGC 2 cut(s) 473, 665
NarI GGCGCC 1 cut(s) 623
NcoI CCATGG 1 cut(s) 856
NdeII GATC 5 cut(s) 152, 266, 425, 637, 748
NlaIII CATG 5 cut(s) 467, 557, 644, 860, 893
NlaIV GGNNCC 1 cut(s) 624
NmuCI GTSAC 1 cut(s) 826
NsiI ATGCAT 1 cut(s) 661
NspI RCATGY 1 cut(s) 557
PagI TCATGA 1 cut(s) 889
PfeI GAWTC 2 cut(s) 104, 530
PflFI GACNNNGTC 1 cut(s) 824
PflMI CCANNNNNTGG 1 cut(s) 760
PkrI GCNGC 5 cut(s) 300, 322, 411, 622, 904
Ple19I CGATCG 1 cut(s) 155
PleI GAGTC 1 cut(s) 408
PluTI GGCGCC 1 cut(s) 626
PpsI GAGTC 1 cut(s) 408
Psp1406I AACGTT 1 cut(s) 495
PspN4I GGNNCC 1 cut(s) 624
PspPI GGNCC 2 cut(s) 183, 508
PsyI GACNNNGTC 1 cut(s) 824
PvuI CGATCG 1 cut(s) 155
RsaI GTAC 2 cut(s) 461, 479
RsaNI GTAC 2 cut(s) 460, 478
RseI CAYNNNNRTG 1 cut(s) 894
SaqAI TTAA 2 cut(s) 291, 416
SatI GCNGC 5 cut(s) 299, 321, 410, 621, 903
Sau3AI GATC 5 cut(s) 152, 266, 425, 637, 748
Sau96I GGNCC 2 cut(s) 183, 508
SchI GAGTC 1 cut(s) 409
SfaNI GCATC 2 cut(s) 646, 889
SfoI GGCGCC 1 cut(s) 624
SmiMI CAYNNNNRTG 1 cut(s) 894
SmlI CTYRAG 1 cut(s) 503
SmoI CTYRAG 1 cut(s) 503
Sse9I AATT 2 cut(s) 90, 755
SsiI CCGC 5 cut(s) 228, 296, 320, 410, 620
SspDI GGCGCC 1 cut(s) 622
SspMI CTAG 1 cut(s) 215
StyI CCWWGG 1 cut(s) 856
TaaI ACNGT 3 cut(s) 72, 163, 471
TaiI ACGT 2 cut(s) 197, 498
TaqI TCGA 8 cut(s) 141, 155, 167, 191, 309, 332, 533, 771
TasI AATT 2 cut(s) 90, 755
TauI GCSGC 3 cut(s) 323, 412, 623
TfiI GAWTC 2 cut(s) 104, 530
Tru1I TTAA 2 cut(s) 291, 416
Tru9I TTAA 2 cut(s) 291, 416
TscAI CASTG 2 cut(s) 348, 474
TseFI GTSAC 1 cut(s) 826
TseI GCWGC 2 cut(s) 298, 902
Tsp45I GTSAC 1 cut(s) 826
TspDTI ATGAA 2 cut(s) 720, 878
TspGWI ACGGA 2 cut(s) 190, 644
TspRI CASTG 2 cut(s) 348, 474
Tth111I GACNNNGTC 1 cut(s) 824
Van91I CCANNNNNTGG 1 cut(s) 760
XagI CCTNNNNNAGG 1 cut(s) 890
XceI RCATGY 1 cut(s) 557
XspI CTAG 1 cut(s) 215
Zsp2I ATGCAT 1 cut(s) 661
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.