Rh5BG485200

Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0003525
Physical Location & Seq
Reverse (-)
77472435 .. 77474719
2285 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG485200.1

Sequence Viewer

Length: 624 bp
ATGCCTTTGACTATAAGCCCTTCTTCAACAACCATAATCTCCCAAAGACCAAACCTTGGTTTCTTCAAAAACACTGGACCAGCTTTCATTAAACTCCCAAAGGGCAACAACAATGGCAACGTAGGTAGTGGTGGTTTGGTAATATTCGCCTCAAATGGGGCTAATAATCGACAAGCACTGGAGGTGGTGTTCGAGCCCTTTGAGGAGGTCAAGAAGGAGCTCCTTCTCGTCCCAACTGATCCTAATGCTTCTCTTGCTCGCCAGAAATACACAGACGAAAGTGTAGCTGCCATTAATGAACAAATCAATGTGGAATACAATGTCTCCTATGTGTACCATGCCATCTATGCCTACTTTGACAGAGACAATGTTGCTCTTAAGGGATTTGCCAAGTTTTTCAAAGAATCAAGCGCAGAAGAAAGGGGGCATGCTGAAAAACTGATGGAATATCAGAACAAACGTGGAGGAAGAGTTAAGCTACAGTCCATGCTAATGCCCTGCTCAGAGTTTGATCATGCTGAGAAGGGTGATGCGTTGCACGCAATGGAGCTTGCGCTTGCTTTGGAAAAGCTGAACAATGAGAAACTTCTAAATCTGCACAAAGTAAGTGACTTTTTACTGTGA

Protein Analysis

207

Amino Acids

23.06

Weight (kDa)

6.23

Isoelectric Point (pI)

33.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ferritin PF00210 96 - 204 2.1e-23 Ferritin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 56
AclWI GGATC 1 cut(s) 233
AfaI GTAC 1 cut(s) 335
AfiI CCNNNNNNNGG 2 cut(s) 56, 156
AflII CTTAAG 1 cut(s) 377
AgsI TTSAA 3 cut(s) 27, 67, 400
AloI GAACNNNNNNTCC 2 cut(s) 173, 205
AluBI AGCT 6 cut(s) 83, 220, 287, 478, 550, 571
AluI AGCT 6 cut(s) 83, 220, 287, 478, 550, 571
Alw21I GWGCWC 1 cut(s) 222
Alw26I GTCTC 2 cut(s) 328, 357
AlwI GGATC 1 cut(s) 233
ApeKI GCWGC 1 cut(s) 287
AseI ATTAAT 1 cut(s) 294
AspLEI GCGC 2 cut(s) 413, 556
AspS9I GGNCC 1 cut(s) 77
AsuHPI GGTGA 1 cut(s) 539
AvaII GGWCC 1 cut(s) 77
BanII GRGCYC 2 cut(s) 198, 222
Bbv12I GWGCWC 1 cut(s) 222
BbvI GCAGC 1 cut(s) 274
BccI CCATC 2 cut(s) 350, 436
BclI TGATCA 1 cut(s) 511
BcoDI GTCTC 2 cut(s) 328, 357
BfmI CTRYAG 1 cut(s) 479
BfrI CTTAAG 1 cut(s) 377
BisI GCNGC 1 cut(s) 288
BlsI GCNGC 1 cut(s) 289
Bme18I GGWCC 1 cut(s) 77
BmgT120I GGNCC 1 cut(s) 77
BmsI GCATC 1 cut(s) 520
BpmI CTGGAG 1 cut(s) 200
BsaJI CCNNGG 1 cut(s) 55
BsaXI ACNNNNNCTCC 6 cut(s) 173, 203, 308, 338, 456, 486
Bsc4I CCNNNNNNNGG 2 cut(s) 56, 156
Bse1I ACTGG 2 cut(s) 79, 183
Bse3DI GCAATG 1 cut(s) 549
BseDI CCNNGG 1 cut(s) 55
BseLI CCNNNNNNNGG 2 cut(s) 56, 156
BseMI GCAATG 1 cut(s) 549
BseMII CTCAG 2 cut(s) 510, 516
BseNI ACTGG 2 cut(s) 79, 183
BseRI GAGGAG 1 cut(s) 218
BseXI GCAGC 1 cut(s) 274
BsgI GTGCAG 1 cut(s) 581
BsiHKAI GWGCWC 1 cut(s) 222
BslFI GGGAC 1 cut(s) 215
BslI CCNNNNNNNGG 2 cut(s) 56, 156
BsmAI GTCTC 2 cut(s) 328, 357
BsmFI GGGAC 1 cut(s) 215
Bsp1286I GDGCHC 2 cut(s) 198, 222
Bsp143I GATC 2 cut(s) 238, 511
BspCNI CTCAG 2 cut(s) 511, 515
BspPI GGATC 1 cut(s) 233
BspTI CTTAAG 1 cut(s) 377
BsrDI GCAATG 1 cut(s) 549
BsrI ACTGG 2 cut(s) 79, 183
BssECI CCNNGG 1 cut(s) 55
BssMI GATC 2 cut(s) 238, 511
BssT1I CCWWGG 1 cut(s) 55
Bst4CI ACNGT 2 cut(s) 483, 621
Bst6I CTCTTC 1 cut(s) 463
BstAFI CTTAAG 1 cut(s) 377
BstC8I GCNNGC 5 cut(s) 259, 429, 540, 552, 558
BstDEI CTNAG 2 cut(s) 502, 519
BstHHI GCGC 2 cut(s) 413, 556
BstKTI GATC 2 cut(s) 241, 514
BstMAI GTCTC 2 cut(s) 328, 357
BstMBI GATC 2 cut(s) 238, 511
BstMWI GCNNNNNNNGC 3 cut(s) 254, 347, 539
BstNSI RCATGY 1 cut(s) 431
BstSFI CTRYAG 1 cut(s) 479
BstV1I GCAGC 1 cut(s) 274
BtsIMutI CAGTG 2 cut(s) 72, 176
Cac8I GCNNGC 5 cut(s) 259, 429, 540, 552, 558
CfoI GCGC 2 cut(s) 413, 556
Cfr13I GGNCC 1 cut(s) 77
Csp6I GTAC 1 cut(s) 334
CviAII CATG 4 cut(s) 338, 428, 487, 515
CviJI RGCY 9 cut(s) 18, 83, 161, 196, 220, 287, 478, 550, 571
CviKI_1 RGCY 9 cut(s) 18, 83, 161, 196, 220, 287, 478, 550, 571
CviQI GTAC 1 cut(s) 334
DdeI CTNAG 2 cut(s) 502, 519
DpnI GATC 2 cut(s) 240, 513
DpnII GATC 2 cut(s) 238, 511
Eam1104I CTCTTC 1 cut(s) 463
EarI CTCTTC 1 cut(s) 463
Ecl136II GAGCTC 1 cut(s) 220
Eco130I CCWWGG 1 cut(s) 55
Eco24I GRGCYC 2 cut(s) 198, 222
Eco47I GGWCC 1 cut(s) 77
Eco53kI GAGCTC 1 cut(s) 220
EcoICRI GAGCTC 1 cut(s) 220
EcoT14I CCWWGG 1 cut(s) 55
EcoT38I GRGCYC 2 cut(s) 198, 222
ErhI CCWWGG 1 cut(s) 55
FaeI CATG 4 cut(s) 341, 431, 490, 518
FaiI YATR 8 cut(s) 14, 35, 330, 339, 348, 429, 488, 516
FalI AAGNNNNNCTT 2 cut(s) 7, 39
FaqI GGGAC 1 cut(s) 215
FatI CATG 4 cut(s) 337, 427, 486, 514
FbaI TGATCA 1 cut(s) 511
Fnu4HI GCNGC 1 cut(s) 288
FriOI GRGCYC 2 cut(s) 198, 222
Fsp4HI GCNGC 1 cut(s) 288
GlaI GCGC 2 cut(s) 412, 555
GluI GCNGC 1 cut(s) 288
GsuI CTGGAG 1 cut(s) 200
HhaI GCGC 2 cut(s) 413, 556
Hin1II CATG 4 cut(s) 341, 431, 490, 518
Hin6I GCGC 2 cut(s) 411, 554
HinP1I GCGC 2 cut(s) 411, 554
HinfI GANTC 1 cut(s) 404
HphI GGTGA 1 cut(s) 539
Hpy166II GTNNAC 1 cut(s) 334
Hpy188I TCNGA 2 cut(s) 453, 505
Hpy188III TCNNGA 1 cut(s) 211
Hpy8I GTNNAC 1 cut(s) 334
HpyAV CCTTC 4 cut(s) 30, 208, 233, 517
HpyCH4III ACNGT 2 cut(s) 483, 621
HpyCH4IV ACGT 2 cut(s) 120, 460
HpyCH4V TGCA 2 cut(s) 538, 598
HpyF10VI GCNNNNNNNGC 3 cut(s) 254, 347, 539
HpyF3I CTNAG 2 cut(s) 502, 519
HpySE526I ACGT 2 cut(s) 120, 460
Hsp92II CATG 4 cut(s) 341, 431, 490, 518
HspAI GCGC 2 cut(s) 411, 554
Ksp22I TGATCA 1 cut(s) 511
Kzo9I GATC 2 cut(s) 238, 511
LmnI GCTCC 3 cut(s) 217, 225, 547
LpnPI CCDG 5 cut(s) 60, 93, 164, 275, 511
Lsp1109I GCAGC 1 cut(s) 274
LweI GCATC 1 cut(s) 520
MaeII ACGT 2 cut(s) 120, 460
MaeIII GTNAC 1 cut(s) 608
MalI GATC 2 cut(s) 240, 513
MboI GATC 2 cut(s) 238, 511
MboII GAAGA 4 cut(s) 15, 55, 428, 480
MhlI GDGCHC 2 cut(s) 198, 222
MnlI CCTC 5 cut(s) 160, 175, 196, 199, 458
MseI TTAA 4 cut(s) 90, 294, 378, 474
MslI CAYNNNNRTG 1 cut(s) 491
MspCI CTTAAG 1 cut(s) 377
MwoI GCNNNNNNNGC 3 cut(s) 254, 347, 539
NdeII GATC 2 cut(s) 238, 511
NlaIII CATG 4 cut(s) 341, 431, 490, 518
NmuCI GTSAC 1 cut(s) 608
NspI RCATGY 1 cut(s) 431
PaeI GCATGC 1 cut(s) 431
PfeI GAWTC 1 cut(s) 404
PflMI CCANNNNNTGG 1 cut(s) 56
PkrI GCNGC 1 cut(s) 289
PshBI ATTAAT 1 cut(s) 294
Psp124BI GAGCTC 1 cut(s) 222
PspPI GGNCC 1 cut(s) 77
RsaI GTAC 1 cut(s) 335
RsaNI GTAC 1 cut(s) 334
RseI CAYNNNNRTG 1 cut(s) 491
SacI GAGCTC 1 cut(s) 222
SaqAI TTAA 4 cut(s) 90, 294, 378, 474
SatI GCNGC 1 cut(s) 288
Sau3AI GATC 2 cut(s) 238, 511
Sau96I GGNCC 1 cut(s) 77
SduI GDGCHC 2 cut(s) 198, 222
SfaNI GCATC 1 cut(s) 520
SfcI CTRYAG 1 cut(s) 479
SinI GGWCC 1 cut(s) 77
SmiMI CAYNNNNRTG 1 cut(s) 491
SmlI CTYRAG 1 cut(s) 377
SmoI CTYRAG 1 cut(s) 377
SphI GCATGC 1 cut(s) 431
SspI AATATT 1 cut(s) 144
SstI GAGCTC 1 cut(s) 222
StyI CCWWGG 1 cut(s) 55
TaaI ACNGT 2 cut(s) 483, 621
TaiI ACGT 2 cut(s) 123, 463
TaqI TCGA 2 cut(s) 169, 192
TfiI GAWTC 1 cut(s) 404
Tru1I TTAA 4 cut(s) 90, 294, 378, 474
Tru9I TTAA 4 cut(s) 90, 294, 378, 474
TscAI CASTG 2 cut(s) 79, 183
TseFI GTSAC 1 cut(s) 608
TseI GCWGC 1 cut(s) 287
Tsp45I GTSAC 1 cut(s) 608
TspDTI ATGAA 2 cut(s) 76, 312
TspRI CASTG 2 cut(s) 79, 183
Van91I CCANNNNNTGG 1 cut(s) 56
Vha464I CTTAAG 1 cut(s) 377
VpaK11BI GGWCC 1 cut(s) 77
VspI ATTAAT 1 cut(s) 294
XceI RCATGY 1 cut(s) 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.