MD12G1178500.v1.1

Ferritin-4, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0003525
Physical Location & Seq
Reverse (-)
25891890 .. 25894048
2159 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1178500.v1.1.491

Sequence Viewer

Length: 798 bp
ATGTCGACGATGTCTCTGAGGGCCATTTCGACGTTTTCCGTCCCATCCAAGCTAGGGGACAAAGGTGGTGCTGTTACCACCCTCTTGTCCAACTCAAAACTTGGATCATCCTCTTCTGCTCTGAGCTTTAAACCGCAGCAAAAACTCGAAAAGTTTGCTGTTTCTGCATCGAGCGAAGCAGTGGCGTTGACCGGCGTCGTGTTTCAGCCGTTTGAGGAGGTGAAGAACGATGCCTTTGTTGTCCCCGTGTCTCCTCAGGTCTCTCTTGCCCGTCAGCGTTACACAGAGGAATCCGAGGCCGCCATTAACGAGCAGATCAATGTGGAATACAATGTGTCGTATGTGTACCATGCACTGTTTGCGTACTTTGACAGGGACAACGTTGCTCTCAAGGGCCTTGCCAAGTTTTTCAAGGAATCAAGCGAGGAAGAAAGAGAACATGCCGAAAAGCTGATGGAATATCAGAACAAGCGAGGTGGGAGAGTGAAACTGCACTCTGTAATCGCGGCGCCTACGGAGTTTGATCATGCAGAAAAGGGAGATGCATTGTATGCTATGGAGTTAGCATTGTCTTTGGAGAAGCTGACAAATGAAAAACTCTTGAACTTGCATAAGGTTGGAGACCAGAACAATGATCCCCAATTGATGGATTTTATCGAGAGCGAGTTTCTGGCTGAGCAGGTTGAGGCCATCAAGAAGATAGCTGACTATGTCAGTCAACTGAGGAGGGTTGGAAAAGGCCATGGAGTGTGGCACTTTGACCAGTATCTCCTTCATGAGGATGATGCTGCAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000041 GO:0000302 GO:0003006 GO:0003674 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006810 GO:0006811 GO:0006812 GO:0006826 GO:0006873 GO:0006875 GO:0006879 GO:0006880 GO:0006950 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009507 GO:0009532 GO:0009534 GO:0009535 GO:0009536 GO:0009570 GO:0009579 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009636 GO:0009719 GO:0009725 GO:0009735 GO:0009791 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010043 GO:0015979 GO:0016020 GO:0019725 GO:0019904 GO:0022414 GO:0030001 GO:0030003 GO:0031976 GO:0031984 GO:0032501 GO:0032502 GO:0034357 GO:0042221 GO:0042493 GO:0042542 GO:0042592 GO:0042651 GO:0043167 GO:0043169 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0046677 GO:0046872 GO:0046914 GO:0046916 GO:0048366 GO:0048367 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0048878 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051235 GO:0051238 GO:0051641 GO:0051651 GO:0051704 GO:0051707 GO:0055035 GO:0055065 GO:0055072 GO:0055076 GO:0055080 GO:0055082 GO:0061458 GO:0065007 GO:0065008 GO:0090567 GO:0097577 GO:0098771 GO:0099402 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko04216 ko04217 ko04978 map04216 map04217 map04978
Pfam Domains
Protein Families
Ferritin

Protein Analysis

266

Amino Acids

29.57

Weight (kDa)

5.35

Isoelectric Point (pI)

41.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ferritin PF00210 100 - 244 4.4e-34 Ferritin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 670
AccB1I GGYRCC 1 cut(s) 508
AccB7I CCANNNNNTGG 1 cut(s) 646
AccI GTMKAC 1 cut(s) 5
AccII CGCG 1 cut(s) 506
AciI CCGC 3 cut(s) 134, 300, 506
AclI AACGTT 1 cut(s) 381
AclWI GGATC 2 cut(s) 112, 629
AcyI GRCGYC 2 cut(s) 195, 509
AfaI GTAC 2 cut(s) 347, 365
AfiI CCNNNNNNNGG 3 cut(s) 54, 646, 778
AgsI TTSAA 2 cut(s) 412, 604
AluBI AGCT 5 cut(s) 52, 126, 451, 583, 704
AluI AGCT 5 cut(s) 52, 126, 451, 583, 704
Alw26I GTCTC 4 cut(s) 18, 255, 265, 615
AlwI GGATC 2 cut(s) 112, 629
AoxI GGCC 5 cut(s) 21, 297, 394, 687, 739
ApeKI GCWGC 2 cut(s) 136, 788
AspLEI GCGC 1 cut(s) 511
AspS9I GGNCC 2 cut(s) 21, 394
AsuHPI GGTGA 1 cut(s) 232
AxyI CCTNAGG 1 cut(s) 255
BanI GGYRCC 1 cut(s) 508
BbvI GCAGC 2 cut(s) 148, 775
BccI CCATC 4 cut(s) 52, 448, 640, 698
BceAI ACGGC 1 cut(s) 193
BcgI CGANNNNNNTGC 2 cut(s) 137, 171
BclI TGATCA 1 cut(s) 523
BcoDI GTCTC 4 cut(s) 18, 255, 265, 615
BfaI CTAG 1 cut(s) 53
BfoI RGCGCY 1 cut(s) 512
BfuAI ACCTGC 1 cut(s) 670
BisI GCNGC 4 cut(s) 137, 300, 507, 789
BlpI GCTNAGC 1 cut(s) 675
BlsI GCNGC 4 cut(s) 138, 301, 508, 790
BmgT120I GGNCC 2 cut(s) 21, 394
BmiI GGNNCC 1 cut(s) 510
BmsI GCATC 4 cut(s) 176, 220, 532, 775
BoxI GACNNNNGTC 1 cut(s) 194
Bpu1102I GCTNAGC 1 cut(s) 675
BpuEI CTTGAG 1 cut(s) 374
BsaHI GRCGYC 2 cut(s) 195, 509
BsaI GGTCTC 2 cut(s) 265, 615
BsaJI CCNNGG 2 cut(s) 294, 742
Bsc4I CCNNNNNNNGG 3 cut(s) 54, 646, 778
Bse118I RCCGGY 1 cut(s) 191
Bse1I ACTGG 1 cut(s) 763
Bse21I CCTNAGG 1 cut(s) 255
BseDI CCNNGG 2 cut(s) 294, 742
BseGI GGATG 3 cut(s) 44, 107, 787
BseLI CCNNNNNNNGG 3 cut(s) 54, 646, 778
BseMII CTCAG 5 cut(s) 8, 113, 269, 666, 713
BseNI ACTGG 1 cut(s) 763
BseRI GAGGAG 3 cut(s) 230, 243, 739
BseXI GCAGC 2 cut(s) 148, 775
BsgI GTGCAG 1 cut(s) 476
Bsh1236I CGCG 1 cut(s) 506
BshFI GGCC 5 cut(s) 23, 299, 396, 689, 741
BshNI GGYRCC 1 cut(s) 508
BsiSI CCGG 1 cut(s) 192
BslFI GGGAC 4 cut(s) 26, 71, 227, 389
BslI CCNNNNNNNGG 3 cut(s) 54, 646, 778
BsmAI GTCTC 4 cut(s) 18, 255, 265, 615
BsmFI GGGAC 4 cut(s) 26, 71, 227, 389
BsnI GGCC 5 cut(s) 23, 299, 396, 689, 741
Bso31I GGTCTC 2 cut(s) 265, 615
Bsp143I GATC 4 cut(s) 104, 315, 523, 634
Bsp1720I GCTNAGC 1 cut(s) 675
Bsp19I CCATGG 1 cut(s) 742
BspACI CCGC 3 cut(s) 134, 300, 506
BspANI GGCC 5 cut(s) 23, 299, 396, 689, 741
BspCNI CTCAG 5 cut(s) 9, 114, 268, 667, 714
BspFNI CGCG 1 cut(s) 506
BspHI TCATGA 1 cut(s) 775
BspLI GGNNCC 1 cut(s) 510
BspMI ACCTGC 1 cut(s) 670
BspPI GGATC 2 cut(s) 112, 629
BspT107I GGYRCC 1 cut(s) 508
BspTNI GGTCTC 2 cut(s) 265, 615
BsrFI RCCGGY 1 cut(s) 191
BsrI ACTGG 1 cut(s) 763
BssAI RCCGGY 1 cut(s) 191
BssECI CCNNGG 2 cut(s) 294, 742
BssMI GATC 4 cut(s) 104, 315, 523, 634
BssNI GRCGYC 2 cut(s) 195, 509
BssT1I CCWWGG 1 cut(s) 742
Bst4CI ACNGT 1 cut(s) 357
Bst6I CTCTTC 1 cut(s) 118
BstACI GRCGYC 2 cut(s) 195, 509
BstAPI GCANNNNNTGC 2 cut(s) 359, 551
BstDEI CTNAG 5 cut(s) 17, 122, 255, 675, 722
BstDSI CCRYGG 1 cut(s) 742
BstENI CCTNNNNNAGG 1 cut(s) 776
BstF5I GGATG 3 cut(s) 44, 107, 787
BstFNI CGCG 1 cut(s) 506
BstH2I RGCGCY 1 cut(s) 512
BstHHI GCGC 1 cut(s) 511
BstKTI GATC 4 cut(s) 107, 318, 526, 637
BstMAI GTCTC 4 cut(s) 18, 255, 265, 615
BstMBI GATC 4 cut(s) 104, 315, 523, 634
BstMWI GCNNNNNNNGC 3 cut(s) 164, 359, 551
BstNSI RCATGY 1 cut(s) 443
BstPAI GACNNNNGTC 1 cut(s) 194
BstUI CGCG 1 cut(s) 506
BstV1I GCAGC 2 cut(s) 148, 775
Bsu36I CCTNAGG 1 cut(s) 255
BsuRI GGCC 5 cut(s) 23, 299, 396, 689, 741
BtgI CCRYGG 1 cut(s) 742
BtsCI GGATG 3 cut(s) 44, 107, 787
BtsI GCAGTG 1 cut(s) 186
BtsIMutI CAGTG 2 cut(s) 186, 353
BveI ACCTGC 1 cut(s) 670
CciI TCATGA 1 cut(s) 775
CfoI GCGC 1 cut(s) 511
Cfr10I RCCGGY 1 cut(s) 191
Cfr13I GGNCC 2 cut(s) 21, 394
CseI GACGC 1 cut(s) 184
Csp6I GTAC 2 cut(s) 346, 364
CspCI CAANNNNNGTGG 2 cut(s) 457, 492
CviAII CATG 5 cut(s) 350, 440, 527, 743, 776
CviQI GTAC 2 cut(s) 346, 364
DdeI CTNAG 5 cut(s) 17, 122, 255, 675, 722
DinI GGCGCC 1 cut(s) 510
DpnI GATC 4 cut(s) 106, 317, 525, 636
DpnII GATC 4 cut(s) 104, 315, 523, 634
DraI TTTAAA 1 cut(s) 130
Eam1104I CTCTTC 1 cut(s) 118
EarI CTCTTC 1 cut(s) 118
Eco130I CCWWGG 1 cut(s) 742
Eco31I GGTCTC 2 cut(s) 265, 615
Eco81I CCTNAGG 1 cut(s) 255
EcoNI CCTNNNNNAGG 1 cut(s) 776
EcoO109I RGGNCCY 1 cut(s) 394
EcoT14I CCWWGG 1 cut(s) 742
EcoT22I ATGCAT 1 cut(s) 547
EgeI GGCGCC 1 cut(s) 510
EheI GGCGCC 1 cut(s) 510
ErhI CCWWGG 1 cut(s) 742
FaeI CATG 5 cut(s) 353, 443, 530, 746, 779
FaqI GGGAC 4 cut(s) 26, 71, 227, 389
FatI CATG 5 cut(s) 349, 439, 526, 742, 775
FbaI TGATCA 1 cut(s) 523
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 4 cut(s) 137, 300, 507, 789
FokI GGATG 3 cut(s) 31, 94, 794
Fsp4HI GCNGC 4 cut(s) 137, 300, 507, 789
FspBI CTAG 1 cut(s) 53
GlaI GCGC 1 cut(s) 510
GluI GCNGC 4 cut(s) 137, 300, 507, 789
HaeII RGCGCY 1 cut(s) 512
HaeIII GGCC 5 cut(s) 23, 299, 396, 689, 741
HapII CCGG 1 cut(s) 192
HgaI GACGC 1 cut(s) 184
HhaI GCGC 1 cut(s) 511
Hin1I GRCGYC 2 cut(s) 195, 509
Hin1II CATG 5 cut(s) 353, 443, 530, 746, 779
Hin6I GCGC 1 cut(s) 509
HinP1I GCGC 1 cut(s) 509
HincII GTYRAC 3 cut(s) 6, 189, 719
HindII GTYRAC 3 cut(s) 6, 189, 719
HinfI GANTC 2 cut(s) 290, 416
HpaII CCGG 1 cut(s) 192
HphI GGTGA 1 cut(s) 232
Hpy166II GTNNAC 4 cut(s) 6, 189, 346, 719
Hpy188I TCNGA 4 cut(s) 18, 123, 295, 465
Hpy188III TCNNGA 4 cut(s) 601, 658, 694, 776
Hpy8I GTNNAC 4 cut(s) 6, 189, 346, 719
Hpy99I CGWCG 3 cut(s) 10, 34, 200
HpyAV CCTTC 1 cut(s) 782
HpyCH4III ACNGT 1 cut(s) 357
HpyCH4IV ACGT 2 cut(s) 32, 381
HpyCH4V TGCA 7 cut(s) 167, 353, 493, 530, 545, 610, 791
HpyF10VI GCNNNNNNNGC 3 cut(s) 164, 359, 551
HpyF3I CTNAG 5 cut(s) 17, 122, 255, 675, 722
HpySE526I ACGT 2 cut(s) 32, 381
Hsp92I GRCGYC 2 cut(s) 195, 509
Hsp92II CATG 5 cut(s) 353, 443, 530, 746, 779
HspAI GCGC 1 cut(s) 509
KasI GGCGCC 1 cut(s) 508
Ksp22I TGATCA 1 cut(s) 523
Kzo9I GATC 4 cut(s) 104, 315, 523, 634
LpnPI CCDG 7 cut(s) 205, 242, 358, 638, 656, 665, 776
Lsp1109I GCAGC 2 cut(s) 148, 775
LweI GCATC 4 cut(s) 176, 220, 532, 775
MaeI CTAG 1 cut(s) 53
MaeII ACGT 2 cut(s) 32, 381
MaeIII GTNAC 2 cut(s) 73, 278
MalI GATC 4 cut(s) 106, 317, 525, 636
MboI GATC 4 cut(s) 104, 315, 523, 634
MboII GAAGA 4 cut(s) 105, 235, 440, 709
MfeI CAATTG 1 cut(s) 641
MluCI AATT 1 cut(s) 641
Mly113I GGCGCC 1 cut(s) 509
MmeI TCCRAC 3 cut(s) 114, 598, 712
Mph1103I ATGCAT 1 cut(s) 547
MseI TTAA 2 cut(s) 129, 306
MslI CAYNNNNRTG 1 cut(s) 780
MspI CCGG 1 cut(s) 192
MunI CAATTG 1 cut(s) 641
MvnI CGCG 1 cut(s) 506
MwoI GCNNNNNNNGC 3 cut(s) 164, 359, 551
NarI GGCGCC 1 cut(s) 509
NcoI CCATGG 1 cut(s) 742
NdeII GATC 4 cut(s) 104, 315, 523, 634
NlaIII CATG 5 cut(s) 353, 443, 530, 746, 779
NlaIV GGNNCC 1 cut(s) 510
NsiI ATGCAT 1 cut(s) 547
NspI RCATGY 1 cut(s) 443
PagI TCATGA 1 cut(s) 775
PfeI GAWTC 2 cut(s) 290, 416
PflFI GACNNNGTC 2 cut(s) 10, 710
PflMI CCANNNNNTGG 1 cut(s) 646
PkrI GCNGC 4 cut(s) 138, 301, 508, 790
PluTI GGCGCC 1 cut(s) 512
PshAI GACNNNNGTC 1 cut(s) 194
Psp1406I AACGTT 1 cut(s) 381
PspN4I GGNNCC 1 cut(s) 510
PspPI GGNCC 2 cut(s) 21, 394
PsyI GACNNNGTC 2 cut(s) 10, 710
RsaI GTAC 2 cut(s) 347, 365
RsaNI GTAC 2 cut(s) 346, 364
RseI CAYNNNNRTG 1 cut(s) 780
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 2 cut(s) 129, 306
SatI GCNGC 4 cut(s) 137, 300, 507, 789
Sau3AI GATC 4 cut(s) 104, 315, 523, 634
Sau96I GGNCC 2 cut(s) 21, 394
SfaNI GCATC 4 cut(s) 176, 220, 532, 775
SfoI GGCGCC 1 cut(s) 510
SmiMI CAYNNNNRTG 1 cut(s) 780
SmlI CTYRAG 1 cut(s) 389
SmoI CTYRAG 1 cut(s) 389
Sse9I AATT 1 cut(s) 641
SsiI CCGC 3 cut(s) 134, 300, 506
SspDI GGCGCC 1 cut(s) 508
SspMI CTAG 1 cut(s) 53
StyI CCWWGG 1 cut(s) 742
TaaI ACNGT 1 cut(s) 357
TaiI ACGT 2 cut(s) 35, 384
TaqI TCGA 5 cut(s) 5, 29, 147, 170, 657
TasI AATT 1 cut(s) 641
TauI GCSGC 2 cut(s) 302, 509
TfiI GAWTC 2 cut(s) 290, 416
Tru1I TTAA 2 cut(s) 129, 306
Tru9I TTAA 2 cut(s) 129, 306
TscAI CASTG 2 cut(s) 186, 360
TseI GCWGC 2 cut(s) 136, 788
TspDTI ATGAA 2 cut(s) 606, 764
TspGWI ACGGA 2 cut(s) 28, 530
TspRI CASTG 2 cut(s) 186, 360
Tth111I GACNNNGTC 2 cut(s) 10, 710
Van91I CCANNNNNTGG 1 cut(s) 646
XagI CCTNNNNNAGG 1 cut(s) 776
XceI RCATGY 1 cut(s) 443
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 1 cut(s) 53
Zsp2I ATGCAT 1 cut(s) 547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.