Rw5G043380

Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0003525
Physical Location & Seq
Reverse (-)
76171599 .. 76173087
1489 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G043380.1

Sequence Viewer

Length: 627 bp
ATGCCTTTGACTATAACCCCTTCTTCAACATCCATAATCTCCCAAAGACCAAACCTTGGTTTCTTCAAAAACAGGGGCACTGGTCCAGCTTTCATCAAACTCCCAAAGAGCAACAACAATGGCAACGTGGGTAGTGGTGGTTTGGTAATATTCGCCTCAAATGGGGCTAATAATCGACAAGCACTGGAGGTGGTGTTCGAGCCCTTTGATAATGCTTCTCTTGCTCGCCAGAAATACACAGACGAAGGTGTAGCTGCCATTAATGAACAAATCAATGTGGAATACAATGTCTCTTATGTGCACCATGCCATGTATGCCTACTTTGACAGAGACAATATTCCTCTTAAGGGATTTGCCAAGTTTTTCAAAGAATCAAGTGCAGAAGAAAGGGGGCATGCTGAAAAACTGATGGAATATCAGAACAAACGTGGAGGAAGAGTTAAGCTACAGTCCATGCTAATGCCCCGCTCAGAGTTTGATCATGCTGAGAAGGGTGATGCGTTGCACGCAATGGAGCTGGCGCTTGCTTTGGAAAAGCTGAACAATGAGAAACTCCTAAATCTGCACAAAAAAGTGCATATTCACCCCAAACCAGCTGTGGTTTATCAGATGATATTCACAGGATGA

Protein Analysis

208

Amino Acids

23.25

Weight (kDa)

9.18

Isoelectric Point (pI)

36.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ferritin PF00210 85 - 193 7.7e-22 Ferritin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 56
AccBSI CCGCTC 1 cut(s) 468
AciI CCGC 1 cut(s) 466
AfiI CCNNNNNNNGG 3 cut(s) 56, 162, 347
AflII CTTAAG 1 cut(s) 344
AgsI TTSAA 3 cut(s) 27, 67, 367
AloI GAACNNNNNNTCC 2 cut(s) 179, 211
AluBI AGCT 6 cut(s) 89, 254, 445, 517, 538, 596
AluI AGCT 6 cut(s) 89, 254, 445, 517, 538, 596
Alw21I GWGCWC 1 cut(s) 303
Alw26I GTCTC 2 cut(s) 295, 324
Alw44I GTGCAC 1 cut(s) 299
ApaLI GTGCAC 1 cut(s) 299
ApeKI GCWGC 1 cut(s) 254
AseI ATTAAT 1 cut(s) 261
AspLEI GCGC 1 cut(s) 523
AspS9I GGNCC 1 cut(s) 83
AsuHPI GGTGA 2 cut(s) 506, 575
AvaII GGWCC 1 cut(s) 83
BaeGI GKGCMC 2 cut(s) 80, 303
BanII GRGCYC 1 cut(s) 204
Bbv12I GWGCWC 1 cut(s) 303
BbvI GCAGC 1 cut(s) 241
BccI CCATC 1 cut(s) 403
BclI TGATCA 1 cut(s) 478
BcoDI GTCTC 2 cut(s) 295, 324
BfmI CTRYAG 1 cut(s) 446
BfoI RGCGCY 1 cut(s) 524
BfrI CTTAAG 1 cut(s) 344
BisI GCNGC 1 cut(s) 255
BlsI GCNGC 1 cut(s) 256
Bme18I GGWCC 1 cut(s) 83
BmgT120I GGNCC 1 cut(s) 83
BmsI GCATC 1 cut(s) 487
BpmI CTGGAG 1 cut(s) 206
BsaJI CCNNGG 1 cut(s) 55
BsaXI ACNNNNNCTCC 4 cut(s) 179, 209, 423, 453
Bsc4I CCNNNNNNNGG 3 cut(s) 56, 162, 347
Bse1I ACTGG 2 cut(s) 85, 189
Bse3DI GCAATG 1 cut(s) 516
BseDI CCNNGG 1 cut(s) 55
BseGI GGATG 1 cut(s) 29
BseLI CCNNNNNNNGG 3 cut(s) 56, 162, 347
BseMI GCAATG 1 cut(s) 516
BseMII CTCAG 2 cut(s) 477, 483
BseNI ACTGG 2 cut(s) 85, 189
BseSI GKGCMC 2 cut(s) 80, 303
BseXI GCAGC 1 cut(s) 241
BsgI GTGCAG 2 cut(s) 399, 548
BsiHKAI GWGCWC 1 cut(s) 303
BslI CCNNNNNNNGG 3 cut(s) 56, 162, 347
BsmAI GTCTC 2 cut(s) 295, 324
Bsp1286I GDGCHC 3 cut(s) 80, 204, 303
Bsp143I GATC 1 cut(s) 478
BspACI CCGC 1 cut(s) 466
BspCNI CTCAG 2 cut(s) 478, 482
BspTI CTTAAG 1 cut(s) 344
BsrBI CCGCTC 1 cut(s) 468
BsrDI GCAATG 1 cut(s) 516
BsrI ACTGG 2 cut(s) 85, 189
BssECI CCNNGG 1 cut(s) 55
BssMI GATC 1 cut(s) 478
BssT1I CCWWGG 1 cut(s) 55
Bst4CI ACNGT 1 cut(s) 450
Bst6I CTCTTC 1 cut(s) 430
BstAFI CTTAAG 1 cut(s) 344
BstC8I GCNNGC 5 cut(s) 226, 396, 507, 519, 525
BstDEI CTNAG 2 cut(s) 469, 486
BstF5I GGATG 1 cut(s) 29
BstH2I RGCGCY 1 cut(s) 524
BstHHI GCGC 1 cut(s) 523
BstKTI GATC 1 cut(s) 481
BstMAI GTCTC 2 cut(s) 295, 324
BstMBI GATC 1 cut(s) 478
BstMWI GCNNNNNNNGC 3 cut(s) 221, 314, 506
BstNSI RCATGY 1 cut(s) 398
BstSFI CTRYAG 1 cut(s) 446
BstSLI GKGCMC 2 cut(s) 80, 303
BstV1I GCAGC 1 cut(s) 241
BtsCI GGATG 1 cut(s) 29
BtsIMutI CAGTG 2 cut(s) 78, 182
Cac8I GCNNGC 5 cut(s) 226, 396, 507, 519, 525
CfoI GCGC 1 cut(s) 523
Cfr13I GGNCC 1 cut(s) 83
CviAII CATG 5 cut(s) 305, 310, 395, 454, 482
CviJI RGCY 8 cut(s) 89, 167, 202, 254, 445, 517, 538, 596
CviKI_1 RGCY 8 cut(s) 89, 167, 202, 254, 445, 517, 538, 596
DdeI CTNAG 2 cut(s) 469, 486
DpnI GATC 1 cut(s) 480
DpnII GATC 1 cut(s) 478
Eam1104I CTCTTC 1 cut(s) 430
EarI CTCTTC 1 cut(s) 430
Eco130I CCWWGG 1 cut(s) 55
Eco24I GRGCYC 1 cut(s) 204
Eco47I GGWCC 1 cut(s) 83
EcoT14I CCWWGG 1 cut(s) 55
EcoT38I GRGCYC 1 cut(s) 204
ErhI CCWWGG 1 cut(s) 55
FaeI CATG 5 cut(s) 308, 313, 398, 457, 485
FatI CATG 5 cut(s) 304, 309, 394, 453, 481
FauI CCCGC 1 cut(s) 473
FbaI TGATCA 1 cut(s) 478
Fnu4HI GCNGC 1 cut(s) 255
FokI GGATG 1 cut(s) 16
FriOI GRGCYC 1 cut(s) 204
Fsp4HI GCNGC 1 cut(s) 255
GlaI GCGC 1 cut(s) 522
GluI GCNGC 1 cut(s) 255
GsuI CTGGAG 1 cut(s) 206
HaeII RGCGCY 1 cut(s) 524
HhaI GCGC 1 cut(s) 523
Hin1II CATG 5 cut(s) 308, 313, 398, 457, 485
Hin6I GCGC 1 cut(s) 521
HinP1I GCGC 1 cut(s) 521
HinfI GANTC 1 cut(s) 371
HphI GGTGA 2 cut(s) 506, 575
Hpy166II GTNNAC 1 cut(s) 301
Hpy188I TCNGA 3 cut(s) 420, 472, 609
Hpy8I GTNNAC 1 cut(s) 301
HpyAV CCTTC 3 cut(s) 30, 239, 484
HpyCH4III ACNGT 1 cut(s) 450
HpyCH4IV ACGT 2 cut(s) 126, 427
HpyCH4V TGCA 5 cut(s) 301, 380, 505, 565, 577
HpyF10VI GCNNNNNNNGC 3 cut(s) 221, 314, 506
HpyF3I CTNAG 2 cut(s) 469, 486
HpySE526I ACGT 2 cut(s) 126, 427
Hsp92II CATG 5 cut(s) 308, 313, 398, 457, 485
HspAI GCGC 1 cut(s) 521
Ksp22I TGATCA 1 cut(s) 478
Kzo9I GATC 1 cut(s) 478
LmnI GCTCC 1 cut(s) 514
LpnPI CCDG 8 cut(s) 58, 66, 99, 170, 242, 503, 606, 606
Lsp1109I GCAGC 1 cut(s) 241
LweI GCATC 1 cut(s) 487
MaeII ACGT 2 cut(s) 126, 427
MalI GATC 1 cut(s) 480
MbiI CCGCTC 1 cut(s) 468
MboI GATC 1 cut(s) 478
MboII GAAGA 4 cut(s) 15, 55, 395, 447
MhlI GDGCHC 3 cut(s) 80, 204, 303
MnlI CCTC 4 cut(s) 166, 181, 351, 425
MseI TTAA 3 cut(s) 261, 345, 441
MslI CAYNNNNRTG 1 cut(s) 458
MspA1I CMGCKG 1 cut(s) 596
MspCI CTTAAG 1 cut(s) 344
MwoI GCNNNNNNNGC 3 cut(s) 221, 314, 506
NdeII GATC 1 cut(s) 478
NlaIII CATG 5 cut(s) 308, 313, 398, 457, 485
NspI RCATGY 1 cut(s) 398
PaeI GCATGC 1 cut(s) 398
PfeI GAWTC 1 cut(s) 371
PflMI CCANNNNNTGG 1 cut(s) 56
PkrI GCNGC 1 cut(s) 256
PshBI ATTAAT 1 cut(s) 261
PspPI GGNCC 1 cut(s) 83
PvuII CAGCTG 1 cut(s) 596
RseI CAYNNNNRTG 1 cut(s) 458
SaqAI TTAA 3 cut(s) 261, 345, 441
SatI GCNGC 1 cut(s) 255
Sau3AI GATC 1 cut(s) 478
Sau96I GGNCC 1 cut(s) 83
SduI GDGCHC 3 cut(s) 80, 204, 303
SfaNI GCATC 1 cut(s) 487
SfcI CTRYAG 1 cut(s) 446
SinI GGWCC 1 cut(s) 83
SmiMI CAYNNNNRTG 1 cut(s) 458
SmlI CTYRAG 1 cut(s) 344
SmoI CTYRAG 1 cut(s) 344
SphI GCATGC 1 cut(s) 398
SsiI CCGC 1 cut(s) 466
SspI AATATT 2 cut(s) 150, 337
StyI CCWWGG 1 cut(s) 55
TaaI ACNGT 1 cut(s) 450
TaiI ACGT 2 cut(s) 129, 430
TaqI TCGA 2 cut(s) 175, 198
TfiI GAWTC 1 cut(s) 371
Tru1I TTAA 3 cut(s) 261, 345, 441
Tru9I TTAA 3 cut(s) 261, 345, 441
TscAI CASTG 2 cut(s) 85, 189
TseI GCWGC 1 cut(s) 254
TspDTI ATGAA 2 cut(s) 82, 279
TspRI CASTG 2 cut(s) 85, 189
Van91I CCANNNNNTGG 1 cut(s) 56
Vha464I CTTAAG 1 cut(s) 344
VneI GTGCAC 1 cut(s) 299
VpaK11BI GGWCC 1 cut(s) 83
VspI ATTAAT 1 cut(s) 261
XceI RCATGY 1 cut(s) 398
XcmI CCANNNNNNNNNTGG 1 cut(s) 595
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.