Rh5AG466700

Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0003525
Physical Location & Seq
Reverse (-)
80169650 .. 80171545
1896 bp
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UTR
Exon/CDS
Intron
Rh5AG466700.1

Sequence Viewer

Length: 564 bp
ATGCCTTTGACTGTAAACCCTTCTTCAACATCCATAATCTCCCAAAGACCAAACCTCAGTTTCTTCAAAAACACTGGACCAGCTTTCATTAAACTCCCAAAGAGCAACAACAATGGCAACGTGGGTAGTGGTGGTTTGGTAACATTCGCCTCAAATGGAGCTAATAATCGACAAGTACTGGAGGTGGTGTTCGAGCCCTTTGAGGAGGTCAAGAAGGAGCTCCTTCTCGTCCCAACTGAGCCTAATGCTTCTCTTGCTCGCCAGAAATACACAGACGAAAGTGTAGCTGCCATTAATGAACAAATCAAGTGGGTTTCAATGGAGCTTGCGCTTGCTTTGGAAAAGCTGAACAATGAGAAACTTCTAAATCTGCACAAAATAGCTAATGAAAACAATGATGTGCATTTAGTGGATTACATTGAAAGTGAATACTTGAATGAACAGGTCGAATCTATAAGGAAAATTTCTGAATATGTAGCTCAGTTGAGAAGGATTGGTCCAGGACATGGTGTTTGGCACTTTGATCAGATGCTCCTTAATGACACAGCCGGGGTCGCCGCATGA

Protein Analysis

187

Amino Acids

20.77

Weight (kDa)

5.42

Isoelectric Point (pI)

32.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ferritin PF00210 107 - 165 8.2e-09 Ferritin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 506
AciI CCGC 1 cut(s) 558
AcsI RAATTY 1 cut(s) 462
AfaI GTAC 1 cut(s) 177
AfiI CCNNNNNNNGG 1 cut(s) 506
AgsI TTSAA 5 cut(s) 27, 67, 318, 422, 436
AjnI CCWGG 1 cut(s) 499
AloI GAACNNNNNNTCC 2 cut(s) 173, 205
AluBI AGCT 8 cut(s) 83, 161, 220, 287, 325, 346, 383, 479
AluI AGCT 8 cut(s) 83, 161, 220, 287, 325, 346, 383, 479
Alw21I GWGCWC 1 cut(s) 222
ApeKI GCWGC 1 cut(s) 287
ApoI RAATTY 1 cut(s) 462
ArsI GACNNNNNNTTYG 2 cut(s) 495, 527
AseI ATTAAT 1 cut(s) 294
AspLEI GCGC 1 cut(s) 331
AspS9I GGNCC 2 cut(s) 77, 497
AsuC2I CCSGG 1 cut(s) 550
AvaII GGWCC 2 cut(s) 77, 497
BanII GRGCYC 2 cut(s) 198, 222
Bbv12I GWGCWC 1 cut(s) 222
BbvI GCAGC 1 cut(s) 274
BciT130I CCWGG 1 cut(s) 501
BclI TGATCA 1 cut(s) 523
BcnI CCSGG 1 cut(s) 550
BisI GCNGC 2 cut(s) 288, 558
BlsI GCNGC 2 cut(s) 289, 559
BmcAI AGTACT 1 cut(s) 177
Bme1390I CCNGG 2 cut(s) 501, 550
Bme18I GGWCC 2 cut(s) 77, 497
BmgT120I GGNCC 2 cut(s) 77, 497
BmrFI CCNGG 2 cut(s) 501, 550
BmsI GCATC 1 cut(s) 519
BpmI CTGGAG 1 cut(s) 200
BpuMI CCSGG 1 cut(s) 550
BsaJI CCNNGG 1 cut(s) 549
BsaXI ACNNNNNCTCC 2 cut(s) 173, 203
Bsc4I CCNNNNNNNGG 1 cut(s) 506
Bse1I ACTGG 2 cut(s) 79, 183
BseBI CCWGG 1 cut(s) 501
BseDI CCNNGG 1 cut(s) 549
BseGI GGATG 1 cut(s) 29
BseLI CCNNNNNNNGG 1 cut(s) 506
BseMII CTCAG 3 cut(s) 70, 228, 494
BseNI ACTGG 2 cut(s) 79, 183
BseRI GAGGAG 1 cut(s) 218
BseXI GCAGC 1 cut(s) 274
BsgI GTGCAG 1 cut(s) 356
BsiHKAI GWGCWC 1 cut(s) 222
BsiSI CCGG 1 cut(s) 549
BslFI GGGAC 1 cut(s) 215
BslI CCNNNNNNNGG 1 cut(s) 506
BsmFI GGGAC 1 cut(s) 215
Bsp1286I GDGCHC 2 cut(s) 198, 222
Bsp143I GATC 1 cut(s) 523
BspACI CCGC 1 cut(s) 558
BspCNI CTCAG 3 cut(s) 69, 229, 493
BsrI ACTGG 2 cut(s) 79, 183
BssECI CCNNGG 1 cut(s) 549
BssMI GATC 1 cut(s) 523
Bst2UI CCWGG 1 cut(s) 501
Bst4CI ACNGT 1 cut(s) 13
BstC8I GCNNGC 3 cut(s) 259, 327, 333
BstDEI CTNAG 3 cut(s) 56, 237, 480
BstF5I GGATG 1 cut(s) 29
BstHHI GCGC 1 cut(s) 331
BstKTI GATC 1 cut(s) 526
BstMBI GATC 1 cut(s) 523
BstMWI GCNNNNNNNGC 2 cut(s) 254, 554
BstNI CCWGG 1 cut(s) 501
BstSCI CCNGG 2 cut(s) 499, 548
BstV1I GCAGC 1 cut(s) 274
BtsCI GGATG 1 cut(s) 29
BtsIMutI CAGTG 1 cut(s) 72
Cac8I GCNNGC 3 cut(s) 259, 327, 333
CfoI GCGC 1 cut(s) 331
Cfr13I GGNCC 2 cut(s) 77, 497
Csp6I GTAC 1 cut(s) 176
CspCI CAANNNNNGTGG 2 cut(s) 290, 325
CviAII CATG 2 cut(s) 506, 561
CviQI GTAC 1 cut(s) 176
DdeI CTNAG 3 cut(s) 56, 237, 480
DpnI GATC 1 cut(s) 525
DpnII GATC 1 cut(s) 523
Ecl136II GAGCTC 1 cut(s) 220
Eco24I GRGCYC 2 cut(s) 198, 222
Eco47I GGWCC 2 cut(s) 77, 497
Eco53kI GAGCTC 1 cut(s) 220
EcoICRI GAGCTC 1 cut(s) 220
EcoRII CCWGG 1 cut(s) 499
EcoT38I GRGCYC 2 cut(s) 198, 222
FaeI CATG 2 cut(s) 509, 564
FaiI YATR 5 cut(s) 35, 455, 474, 507, 562
FaqI GGGAC 1 cut(s) 215
FatI CATG 2 cut(s) 505, 560
FbaI TGATCA 1 cut(s) 523
Fnu4HI GCNGC 2 cut(s) 288, 558
FokI GGATG 1 cut(s) 16
FriOI GRGCYC 2 cut(s) 198, 222
Fsp4HI GCNGC 2 cut(s) 288, 558
GlaI GCGC 1 cut(s) 330
GluI GCNGC 2 cut(s) 288, 558
GsuI CTGGAG 1 cut(s) 200
HapII CCGG 1 cut(s) 549
HhaI GCGC 1 cut(s) 331
Hin1II CATG 2 cut(s) 509, 564
Hin6I GCGC 1 cut(s) 329
HinP1I GCGC 1 cut(s) 329
HinfI GANTC 1 cut(s) 449
HpaII CCGG 1 cut(s) 549
Hpy166II GTNNAC 1 cut(s) 16
Hpy188I TCNGA 2 cut(s) 469, 528
Hpy188III TCNNGA 1 cut(s) 211
Hpy8I GTNNAC 1 cut(s) 16
HpyAV CCTTC 4 cut(s) 30, 208, 233, 483
HpyCH4III ACNGT 1 cut(s) 13
HpyCH4IV ACGT 1 cut(s) 120
HpyCH4V TGCA 2 cut(s) 373, 403
HpyF10VI GCNNNNNNNGC 2 cut(s) 254, 554
HpyF3I CTNAG 3 cut(s) 56, 237, 480
HpySE526I ACGT 1 cut(s) 120
Hsp92II CATG 2 cut(s) 509, 564
HspAI GCGC 1 cut(s) 329
Ksp22I TGATCA 1 cut(s) 523
Kzo9I GATC 1 cut(s) 523
LmnI GCTCC 5 cut(s) 158, 217, 225, 322, 537
LpnPI CCDG 7 cut(s) 60, 93, 164, 275, 428, 486, 513
Lsp1109I GCAGC 1 cut(s) 274
LweI GCATC 1 cut(s) 519
MaeII ACGT 1 cut(s) 120
MaeIII GTNAC 1 cut(s) 139
MalI GATC 1 cut(s) 525
MboI GATC 1 cut(s) 523
MboII GAAGA 2 cut(s) 15, 55
MhlI GDGCHC 2 cut(s) 198, 222
MluCI AATT 1 cut(s) 462
MnlI CCTC 5 cut(s) 65, 160, 175, 196, 199
MseI TTAA 3 cut(s) 90, 294, 537
MspI CCGG 1 cut(s) 549
MspR9I CCNGG 2 cut(s) 501, 550
MvaI CCWGG 1 cut(s) 501
MwoI GCNNNNNNNGC 2 cut(s) 254, 554
NciI CCSGG 1 cut(s) 550
NdeII GATC 1 cut(s) 523
NlaIII CATG 2 cut(s) 509, 564
PfeI GAWTC 1 cut(s) 449
PflMI CCANNNNNTGG 1 cut(s) 506
PfoI TCCNGGA 1 cut(s) 499
PkrI GCNGC 2 cut(s) 289, 559
PshBI ATTAAT 1 cut(s) 294
Psp124BI GAGCTC 1 cut(s) 222
Psp6I CCWGG 1 cut(s) 499
PspGI CCWGG 1 cut(s) 499
PspPI GGNCC 2 cut(s) 77, 497
RsaI GTAC 1 cut(s) 177
RsaNI GTAC 1 cut(s) 176
SacI GAGCTC 1 cut(s) 222
SaqAI TTAA 3 cut(s) 90, 294, 537
SatI GCNGC 2 cut(s) 288, 558
Sau3AI GATC 1 cut(s) 523
Sau96I GGNCC 2 cut(s) 77, 497
ScaI AGTACT 1 cut(s) 177
ScrFI CCNGG 2 cut(s) 501, 550
SduI GDGCHC 2 cut(s) 198, 222
SfaNI GCATC 1 cut(s) 519
SinI GGWCC 2 cut(s) 77, 497
Sse9I AATT 1 cut(s) 462
SsiI CCGC 1 cut(s) 558
SstI GAGCTC 1 cut(s) 222
StyD4I CCNGG 2 cut(s) 499, 548
TaaI ACNGT 1 cut(s) 13
TaiI ACGT 1 cut(s) 123
TaqI TCGA 3 cut(s) 169, 192, 447
TasI AATT 1 cut(s) 462
TatI WGTACW 1 cut(s) 175
TauI GCSGC 1 cut(s) 560
TfiI GAWTC 1 cut(s) 449
Tru1I TTAA 3 cut(s) 90, 294, 537
Tru9I TTAA 3 cut(s) 90, 294, 537
TscAI CASTG 1 cut(s) 79
TseI GCWGC 1 cut(s) 287
TspDTI ATGAA 4 cut(s) 76, 312, 402, 453
TspRI CASTG 1 cut(s) 79
Van91I CCANNNNNTGG 1 cut(s) 506
VpaK11BI GGWCC 2 cut(s) 77, 497
VspI ATTAAT 1 cut(s) 294
XapI RAATTY 1 cut(s) 462
ZrmI AGTACT 1 cut(s) 177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.