MD04G1164500.v1.1

Ferritin-4, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0003525
Physical Location & Seq
Reverse (-)
25512525 .. 25515120
2596 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1164500.v1.1.491

Sequence Viewer

Length: 789 bp
ATGTCTCTGAGGTCCGTTTCGACATTTTCGGTCCCATCCAAGCAACGGGACAATGGTGGTGCTGTTAGCACCCTCTTGTTCAACTCAAAACTTGGATCACCCTCCTCCGCTCTGAGCTTCAAGCCGCAGCGAAAACTCGAAAAGTTTTCGGTTTCTGCATCGAGAGATGCAGTGGCGATGACCGGCGTGGTGTTTCAGCCGTTTGAGGAGGTGAAGAACGATGCTTTTGTTGTCCCCGCGGCTCCTCATGTCTCTCTTGCCCGTCAGCGTTACGCAAAAGAGTCCGAAGCCGCCATAAACGAGCAGATCAATGTGGAGTACAATGTGTCGTACGTGTACCATGCACTGTTTGCGTACTTTGACAGGGACAACATTGCTCTCAAGGGCCTTGCCAAGTTTTTCAAGGACTCGAGCGAGGAAGAAAGAGAACATGCCGAAAAGCTGATGGAATATCAGAATATGCGAGGTGGGAGAGTGACACTGCACTCCATAATAGCGCCGCCTACGGAGTTTGATCATGTAGAGAAGGGAGATGCATTGTATGCTATGGAGTTAGCATTGTCTTTGGAGAAGCTGACAAATGAAAAGCTCTTGAACTTGCATAAGGTTGCAGACGAGAACAATGATGCCCAATTGACAGACTTTATCGAGAGCGAGTTTCTGGCTGAGCAGGTTGAAGCCATTAAGAAGATAGCTGACTATGTCACTCAACTGAGAATGGTTGGAAAAGGCCATGGAGTGTGGCACTTTGACCAACAGCTCCTTCACGAGGGTGATGCTGCAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000041 GO:0000302 GO:0003006 GO:0003674 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006810 GO:0006811 GO:0006812 GO:0006826 GO:0006873 GO:0006875 GO:0006879 GO:0006880 GO:0006950 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009507 GO:0009532 GO:0009534 GO:0009535 GO:0009536 GO:0009570 GO:0009579 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009636 GO:0009719 GO:0009725 GO:0009735 GO:0009791 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010043 GO:0015979 GO:0016020 GO:0019725 GO:0019904 GO:0022414 GO:0030001 GO:0030003 GO:0031976 GO:0031984 GO:0032501 GO:0032502 GO:0034357 GO:0042221 GO:0042493 GO:0042542 GO:0042592 GO:0042651 GO:0043167 GO:0043169 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0046677 GO:0046872 GO:0046914 GO:0046916 GO:0048366 GO:0048367 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0048878 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051235 GO:0051238 GO:0051641 GO:0051651 GO:0051704 GO:0051707 GO:0055035 GO:0055065 GO:0055072 GO:0055076 GO:0055080 GO:0055082 GO:0061458 GO:0065007 GO:0065008 GO:0090567 GO:0097577 GO:0098771 GO:0099402 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko04216 ko04217 ko04978 map04216 map04217 map04978
Pfam Domains
Protein Families
Ferritin

Protein Analysis

263

Amino Acids

29.36

Weight (kDa)

5.52

Isoelectric Point (pI)

37.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ferritin PF00210 97 - 240 2.8e-35 Ferritin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 661
AccBSI CCGCTC 1 cut(s) 110
AccII CGCG 1 cut(s) 239
AciI CCGC 6 cut(s) 108, 125, 237, 239, 291, 500
AclWI GGATC 1 cut(s) 103
AfaI GTAC 4 cut(s) 320, 332, 338, 356
AfiI CCNNNNNNNGG 2 cut(s) 45, 769
AflIII ACRYGT 1 cut(s) 333
AgsI TTSAA 5 cut(s) 82, 121, 403, 595, 677
AjuI GAANNNNNNNTTGG 2 cut(s) 747, 779
AleI CACNNNNGTG 1 cut(s) 771
AluBI AGCT 6 cut(s) 117, 442, 574, 589, 695, 760
AluI AGCT 6 cut(s) 117, 442, 574, 589, 695, 760
Alw26I GTCTC 2 cut(s) 9, 256
AlwI GGATC 1 cut(s) 103
Ama87I CYCGRG 1 cut(s) 409
AoxI GGCC 2 cut(s) 385, 730
ApeKI GCWGC 2 cut(s) 127, 779
AspLEI GCGC 1 cut(s) 499
AspS9I GGNCC 3 cut(s) 12, 31, 385
AsuHPI GGTGA 3 cut(s) 90, 223, 785
AvaI CYCGRG 1 cut(s) 409
AvaII GGWCC 2 cut(s) 12, 31
BauI CACGAG 1 cut(s) 767
BbvI GCAGC 2 cut(s) 139, 766
BccI CCATC 2 cut(s) 43, 439
BceAI ACGGC 1 cut(s) 184
BcgI CGANNNNNNTGC 1 cut(s) 758
BclI TGATCA 1 cut(s) 514
BcoDI GTCTC 2 cut(s) 9, 256
BfoI RGCGCY 1 cut(s) 500
BfuAI ACCTGC 1 cut(s) 661
BisI GCNGC 6 cut(s) 125, 128, 240, 291, 500, 780
BlpI GCTNAGC 1 cut(s) 666
BlsI GCNGC 6 cut(s) 126, 129, 241, 292, 501, 781
Bme18I GGWCC 2 cut(s) 12, 31
BmeT110I CYCGRG 1 cut(s) 409
BmgT120I GGNCC 3 cut(s) 12, 31, 385
BmiI GGNNCC 2 cut(s) 33, 243
BmsI GCATC 6 cut(s) 157, 167, 211, 523, 616, 766
Bpu1102I GCTNAGC 1 cut(s) 666
BpuEI CTTGAG 1 cut(s) 365
BsaAI YACGTR 1 cut(s) 334
BsaJI CCNNGG 2 cut(s) 237, 733
Bsc4I CCNNNNNNNGG 2 cut(s) 45, 769
Bse118I RCCGGY 1 cut(s) 182
Bse3DI GCAATG 1 cut(s) 372
BseDI CCNNGG 2 cut(s) 237, 733
BseGI GGATG 1 cut(s) 35
BseLI CCNNNNNNNGG 2 cut(s) 45, 769
BseMI GCAATG 1 cut(s) 372
BseMII CTCAG 3 cut(s) 104, 657, 704
BseRI GAGGAG 3 cut(s) 94, 221, 234
BseXI GCAGC 2 cut(s) 139, 766
BsgI GTGCAG 1 cut(s) 467
Bsh1236I CGCG 1 cut(s) 239
BshFI GGCC 2 cut(s) 387, 732
BsiHKCI CYCGRG 1 cut(s) 409
BsiSI CCGG 1 cut(s) 183
BsiWI CGTACG 1 cut(s) 330
BslFI GGGAC 4 cut(s) 17, 62, 218, 380
BslI CCNNNNNNNGG 2 cut(s) 45, 769
BsmAI GTCTC 2 cut(s) 9, 256
BsmFI GGGAC 4 cut(s) 17, 62, 218, 380
BsnI GGCC 2 cut(s) 387, 732
BsoBI CYCGRG 1 cut(s) 409
Bsp143I GATC 3 cut(s) 95, 306, 514
Bsp1720I GCTNAGC 1 cut(s) 666
Bsp19I CCATGG 1 cut(s) 733
BspACI CCGC 6 cut(s) 108, 125, 237, 239, 291, 500
BspANI GGCC 2 cut(s) 387, 732
BspCNI CTCAG 3 cut(s) 105, 658, 705
BspFNI CGCG 1 cut(s) 239
BspLI GGNNCC 2 cut(s) 33, 243
BspMI ACCTGC 1 cut(s) 661
BspPI GGATC 1 cut(s) 103
BsrBI CCGCTC 1 cut(s) 110
BsrDI GCAATG 1 cut(s) 372
BsrFI RCCGGY 1 cut(s) 182
BssAI RCCGGY 1 cut(s) 182
BssECI CCNNGG 2 cut(s) 237, 733
BssMI GATC 3 cut(s) 95, 306, 514
BssSI CACGAG 1 cut(s) 767
BssT1I CCWWGG 1 cut(s) 733
Bst2BI CACGAG 1 cut(s) 767
Bst4CI ACNGT 1 cut(s) 348
BstAPI GCANNNNNTGC 2 cut(s) 350, 542
BstBAI YACGTR 1 cut(s) 334
BstDEI CTNAG 4 cut(s) 8, 113, 666, 713
BstDSI CCRYGG 2 cut(s) 237, 733
BstENI CCTNNNNNAGG 1 cut(s) 767
BstF5I GGATG 1 cut(s) 35
BstFNI CGCG 1 cut(s) 239
BstH2I RGCGCY 1 cut(s) 500
BstHHI GCGC 1 cut(s) 499
BstKTI GATC 3 cut(s) 98, 309, 517
BstMAI GTCTC 2 cut(s) 9, 256
BstMBI GATC 3 cut(s) 95, 306, 514
BstMWI GCNNNNNNNGC 2 cut(s) 350, 542
BstNSI RCATGY 1 cut(s) 434
BstUI CGCG 1 cut(s) 239
BstV1I GCAGC 2 cut(s) 139, 766
BsuRI GGCC 2 cut(s) 387, 732
BtgI CCRYGG 2 cut(s) 237, 733
BtgZI GCGATG 1 cut(s) 191
BtsCI GGATG 1 cut(s) 35
BtsI GCAGTG 2 cut(s) 177, 479
BtsIMutI CAGTG 3 cut(s) 177, 344, 479
BveI ACCTGC 1 cut(s) 661
CfoI GCGC 1 cut(s) 499
Cfr10I RCCGGY 1 cut(s) 182
Cfr13I GGNCC 3 cut(s) 12, 31, 385
Cfr42I CCGCGG 1 cut(s) 240
Csp6I GTAC 4 cut(s) 319, 331, 337, 355
CviAII CATG 5 cut(s) 248, 341, 431, 518, 734
CviQI GTAC 4 cut(s) 319, 331, 337, 355
DdeI CTNAG 4 cut(s) 8, 113, 666, 713
DpnI GATC 3 cut(s) 97, 308, 516
DpnII GATC 3 cut(s) 95, 306, 514
Eco130I CCWWGG 1 cut(s) 733
Eco47I GGWCC 2 cut(s) 12, 31
Eco88I CYCGRG 1 cut(s) 409
EcoNI CCTNNNNNAGG 1 cut(s) 767
EcoO109I RGGNCCY 1 cut(s) 385
EcoT14I CCWWGG 1 cut(s) 733
EcoT22I ATGCAT 1 cut(s) 538
ErhI CCWWGG 1 cut(s) 733
FaeI CATG 5 cut(s) 251, 344, 434, 521, 737
FaqI GGGAC 4 cut(s) 17, 62, 218, 380
FatI CATG 5 cut(s) 247, 340, 430, 517, 733
FauI CCCGC 1 cut(s) 244
FbaI TGATCA 1 cut(s) 514
Fnu4HI GCNGC 6 cut(s) 125, 128, 240, 291, 500, 780
FokI GGATG 1 cut(s) 22
Fsp4HI GCNGC 6 cut(s) 125, 128, 240, 291, 500, 780
GlaI GCGC 1 cut(s) 498
GluI GCNGC 6 cut(s) 125, 128, 240, 291, 500, 780
HaeII RGCGCY 1 cut(s) 500
HaeIII GGCC 2 cut(s) 387, 732
HapII CCGG 1 cut(s) 183
HhaI GCGC 1 cut(s) 499
Hin1II CATG 5 cut(s) 251, 344, 434, 521, 737
Hin6I GCGC 1 cut(s) 497
HinP1I GCGC 1 cut(s) 497
HinfI GANTC 2 cut(s) 281, 407
HpaII CCGG 1 cut(s) 183
HphI GGTGA 3 cut(s) 90, 223, 785
Hpy166II GTNNAC 1 cut(s) 337
Hpy188I TCNGA 4 cut(s) 9, 114, 286, 456
Hpy188III TCNNGA 4 cut(s) 162, 592, 649, 767
Hpy8I GTNNAC 1 cut(s) 337
HpyAV CCTTC 2 cut(s) 520, 773
HpyCH4III ACNGT 1 cut(s) 348
HpyCH4IV ACGT 1 cut(s) 333
HpyCH4V TGCA 8 cut(s) 158, 170, 344, 484, 536, 601, 611, 782
HpyF10VI GCNNNNNNNGC 2 cut(s) 350, 542
HpyF3I CTNAG 4 cut(s) 8, 113, 666, 713
HpySE526I ACGT 1 cut(s) 333
Hsp92II CATG 5 cut(s) 251, 344, 434, 521, 737
HspAI GCGC 1 cut(s) 497
Ksp22I TGATCA 1 cut(s) 514
KspI CCGCGG 1 cut(s) 240
Kzo9I GATC 3 cut(s) 95, 306, 514
LmnI GCTCC 2 cut(s) 247, 765
LpnPI CCDG 4 cut(s) 196, 349, 647, 656
Lsp1109I GCAGC 2 cut(s) 139, 766
LweI GCATC 6 cut(s) 157, 167, 211, 523, 616, 766
MaeII ACGT 1 cut(s) 333
MaeIII GTNAC 3 cut(s) 269, 475, 703
MalI GATC 3 cut(s) 97, 308, 516
MbiI CCGCTC 1 cut(s) 110
MboI GATC 3 cut(s) 95, 306, 514
MboII GAAGA 3 cut(s) 226, 431, 700
MfeI CAATTG 1 cut(s) 632
MluCI AATT 2 cut(s) 632, 784
MlyI GAGTC 2 cut(s) 290, 401
MmeI TCCRAC 1 cut(s) 703
Mph1103I ATGCAT 1 cut(s) 538
MseI TTAA 2 cut(s) 684, 787
MslI CAYNNNNRTG 1 cut(s) 771
MspA1I CMGCKG 1 cut(s) 239
MspI CCGG 1 cut(s) 183
MunI CAATTG 1 cut(s) 632
MvnI CGCG 1 cut(s) 239
MwoI GCNNNNNNNGC 2 cut(s) 350, 542
NcoI CCATGG 1 cut(s) 733
NdeII GATC 3 cut(s) 95, 306, 514
NlaIII CATG 5 cut(s) 251, 344, 434, 521, 737
NlaIV GGNNCC 2 cut(s) 33, 243
NmuCI GTSAC 2 cut(s) 475, 703
NsiI ATGCAT 1 cut(s) 538
NspI RCATGY 1 cut(s) 434
OliI CACNNNNGTG 1 cut(s) 771
PaeR7I CTCGAG 1 cut(s) 409
Pfl23II CGTACG 1 cut(s) 330
PflFI GACNNNGTC 1 cut(s) 701
PkrI GCNGC 6 cut(s) 126, 129, 241, 292, 501, 781
PleI GAGTC 2 cut(s) 289, 401
PpsI GAGTC 2 cut(s) 289, 401
Ppu21I YACGTR 1 cut(s) 334
PspLI CGTACG 1 cut(s) 330
PspN4I GGNNCC 2 cut(s) 33, 243
PspPI GGNCC 3 cut(s) 12, 31, 385
PspXI VCTCGAGB 1 cut(s) 409
PsyI GACNNNGTC 1 cut(s) 701
RsaI GTAC 4 cut(s) 320, 332, 338, 356
RsaNI GTAC 4 cut(s) 319, 331, 337, 355
RseI CAYNNNNRTG 1 cut(s) 771
SacII CCGCGG 1 cut(s) 240
SaqAI TTAA 2 cut(s) 684, 787
SatI GCNGC 6 cut(s) 125, 128, 240, 291, 500, 780
Sau3AI GATC 3 cut(s) 95, 306, 514
Sau96I GGNCC 3 cut(s) 12, 31, 385
SchI GAGTC 2 cut(s) 290, 401
SfaNI GCATC 6 cut(s) 157, 167, 211, 523, 616, 766
Sfr274I CTCGAG 1 cut(s) 409
Sfr303I CCGCGG 1 cut(s) 240
SgrBI CCGCGG 1 cut(s) 240
SinI GGWCC 2 cut(s) 12, 31
SlaI CTCGAG 1 cut(s) 409
SmiMI CAYNNNNRTG 1 cut(s) 771
SmlI CTYRAG 2 cut(s) 380, 409
SmoI CTYRAG 2 cut(s) 380, 409
Sse9I AATT 2 cut(s) 632, 784
SsiI CCGC 6 cut(s) 108, 125, 237, 239, 291, 500
StyI CCWWGG 1 cut(s) 733
TaaI ACNGT 1 cut(s) 348
TaiI ACGT 1 cut(s) 336
TaqI TCGA 5 cut(s) 20, 138, 161, 410, 648
TaqII GACCGA 1 cut(s) 19
TasI AATT 2 cut(s) 632, 784
TatI WGTACW 1 cut(s) 318
TauI GCSGC 4 cut(s) 127, 242, 293, 502
Tru1I TTAA 2 cut(s) 684, 787
Tru9I TTAA 2 cut(s) 684, 787
TscAI CASTG 3 cut(s) 177, 351, 486
TseFI GTSAC 2 cut(s) 475, 703
TseI GCWGC 2 cut(s) 127, 779
Tsp45I GTSAC 2 cut(s) 475, 703
TspDTI ATGAA 1 cut(s) 597
TspGWI ACGGA 2 cut(s) 4, 521
TspRI CASTG 3 cut(s) 177, 351, 486
Tth111I GACNNNGTC 1 cut(s) 701
VpaK11BI GGWCC 2 cut(s) 12, 31
XagI CCTNNNNNAGG 1 cut(s) 767
XceI RCATGY 1 cut(s) 434
XhoI CTCGAG 1 cut(s) 409
Zsp2I ATGCAT 1 cut(s) 538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.