MD03G1199000.v1.1

lactoylglutathione

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002543
Physical Location & Seq
Forward (+)
27235904 .. 27239838
3935 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1199000.v1.1.491

Sequence Viewer

Length: 909 bp
ATGGTTCAGTTTGTAGCTCGGGCAGTACCCCATGATGATGTGTTAGAATGGATACAGAAAGATAATCATCGTTTCCTCCGTGCCAATATCCGAGTTGGTGATCTCGATCGCACCATTAAGTTTTACACAGAATTTTTTGGAATGAAAGTACTGAGTAGAAAAGATTTCCCAGCAGAAAAATACTCGAATGCAATCGTTGGGTTTGGACCGGAAGAATCTCAGTTTGTGATAGGGCTCACAGACCATCACAATGCAGAGGACGAGCTAGACATTGGGACAGCCTTTAGTCATTTTGGATTTTCAACCCAAGATATTTACAACACGGTTGAAAAAGTTCGAGCTAGTGGTGGTGTGATCACTCGTGAATCTGGGCCGCTCGTAGAAGGGGGAGAGGGAACCGTTTTCGCCTTCATGAACGATCCTGACGGCTACAGTTTCGAGCTCCTCCAGAGGCCGCCAACTCCACAACCACTTTCTCATATATGTCTTAATGTTCTTGATTTAGATCGCTCTATTGAGTTCTACAACAAGTCCTTGGGCATGAACCTACTACAGACGTTTGACTTCCCTCAAGAGCGGTATACCGTAGGTATGGTGGGGTACGGATCCAATCTTACTCAAACAGTTGTTATCGAGTTGAAATACAATTATAATGTGACGGCATATAGAAGAGGAAATGGTTATGCACAGGTTGCTATTGGCACTAATGATGTGTACAAGAGCGCAGCAGCTGTAAGACGGGTAACCAAGGAAGTTGGAGGGGAAATAATAATACCACCAGGTCCAATCCCAAAAATCGACGCCAAAATCACTGCACTCCTTGATACAGATGGCTTTCAAACGGTGTTGGTAGACAATGAAGACTACTTGAAAAGAATTGAAGAAAGAGAGATGAAGCAAAATCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00620 map00620
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

303

Amino Acids

33.99

Weight (kDa)

5.09

Isoelectric Point (pI)

35.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 26 - 148 9.6e-18 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase_4 PF13669 30 - 137 8.5e-06 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase PF00903 158 - 280 3.2e-09 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 651
AccBSI CCGCTC 2 cut(s) 376, 577
AccI GTMKAC 2 cut(s) 581, 852
AciI CCGC 3 cut(s) 374, 455, 577
AclWI GGATC 3 cut(s) 413, 600, 613
AcsI RAATTY 1 cut(s) 131
AcyI GRCGYC 1 cut(s) 801
AfaI GTAC 4 cut(s) 27, 150, 602, 716
AgsI TTSAA 6 cut(s) 303, 329, 640, 839, 871, 881
AjnI CCWGG 1 cut(s) 778
AluBI AGCT 5 cut(s) 17, 265, 341, 442, 731
AluI AGCT 5 cut(s) 17, 265, 341, 442, 731
Alw21I GWGCWC 1 cut(s) 444
AlwI GGATC 3 cut(s) 413, 600, 613
AlwNI CAGNNNCTG 1 cut(s) 731
Ama87I CYCGRG 1 cut(s) 18
AoxI GGCC 2 cut(s) 371, 452
ApeKI GCWGC 2 cut(s) 725, 728
ApoI RAATTY 1 cut(s) 131
Asp700I GAANNNNTTC 1 cut(s) 333
AspLEI GCGC 1 cut(s) 725
AspS9I GGNCC 3 cut(s) 206, 371, 782
AsuHPI GGTGA 1 cut(s) 110
AvaI CYCGRG 1 cut(s) 18
AvaII GGWCC 2 cut(s) 206, 782
BamHI GGATCC 1 cut(s) 605
BanII GRGCYC 2 cut(s) 237, 444
BauI CACGAG 1 cut(s) 360
BbsI GAAGAC 1 cut(s) 867
Bbv12I GWGCWC 1 cut(s) 444
BbvI GCAGC 2 cut(s) 737, 740
BccI CCATC 2 cut(s) 252, 824
BceAI ACGGC 2 cut(s) 442, 675
BciT130I CCWGG 1 cut(s) 780
BciVI GTATCC 1 cut(s) 45
BclI TGATCA 1 cut(s) 354
BfaI CTAG 2 cut(s) 266, 342
BfmI CTRYAG 2 cut(s) 430, 551
BfuI GTATCC 1 cut(s) 45
BisI GCNGC 4 cut(s) 374, 455, 726, 729
BlsI GCNGC 4 cut(s) 375, 456, 727, 730
BmcAI AGTACT 1 cut(s) 150
Bme1390I CCNGG 1 cut(s) 780
Bme18I GGWCC 2 cut(s) 206, 782
BmeT110I CYCGRG 1 cut(s) 18
BmgT120I GGNCC 3 cut(s) 206, 371, 782
BmiI GGNNCC 2 cut(s) 397, 607
BmrFI CCNGG 1 cut(s) 780
BpiI GAAGAC 1 cut(s) 867
BpmI CTGGAG 1 cut(s) 431
BpuEI CTTGAG 1 cut(s) 555
BsaBI GATNNNNATC 3 cut(s) 66, 105, 504
BsaHI GRCGYC 1 cut(s) 801
BsaJI CCNNGG 2 cut(s) 534, 747
BsaWI WCCGGW 1 cut(s) 208
Bse8I GATNNNNATC 3 cut(s) 66, 105, 504
BseBI CCWGG 1 cut(s) 780
BseDI CCNNGG 2 cut(s) 534, 747
BseJI GATNNNNATC 3 cut(s) 66, 105, 504
BseMII CTCAG 2 cut(s) 143, 233
BseRI GAGGAG 1 cut(s) 434
BseXI GCAGC 2 cut(s) 737, 740
BseYI CCCAGC 1 cut(s) 169
BsgI GTGCAG 1 cut(s) 798
Bsh1285I CGRYCG 1 cut(s) 109
BshFI GGCC 2 cut(s) 373, 454
BsiEI CGRYCG 1 cut(s) 109
BsiHKAI GWGCWC 1 cut(s) 444
BsiHKCI CYCGRG 1 cut(s) 18
BsiSI CCGG 1 cut(s) 209
BslFI GGGAC 1 cut(s) 289
BsmFI GGGAC 1 cut(s) 289
BsmI GAATGC 1 cut(s) 193
BsnI GGCC 2 cut(s) 373, 454
BsoBI CYCGRG 1 cut(s) 18
Bsp1286I GDGCHC 2 cut(s) 237, 444
Bsp1407I TGTACA 1 cut(s) 714
Bsp143I GATC 6 cut(s) 100, 106, 354, 418, 505, 605
BspACI CCGC 3 cut(s) 374, 455, 577
BspANI GGCC 2 cut(s) 373, 454
BspCNI CTCAG 2 cut(s) 144, 232
BspHI TCATGA 1 cut(s) 411
BspLI GGNNCC 2 cut(s) 397, 607
BspPI GGATC 3 cut(s) 413, 600, 613
BsrBI CCGCTC 2 cut(s) 376, 577
BsrGI TGTACA 1 cut(s) 714
BssECI CCNNGG 2 cut(s) 534, 747
BssMI GATC 6 cut(s) 100, 106, 354, 418, 505, 605
BssNAI GTATAC 1 cut(s) 582
BssNI GRCGYC 1 cut(s) 801
BssSI CACGAG 1 cut(s) 360
BssT1I CCWWGG 2 cut(s) 534, 747
Bst1107I GTATAC 1 cut(s) 582
Bst2BI CACGAG 1 cut(s) 360
Bst2UI CCWGG 1 cut(s) 780
Bst4CI ACNGT 6 cut(s) 325, 400, 434, 586, 625, 844
Bst6I CTCTTC 1 cut(s) 664
BstACI GRCGYC 1 cut(s) 801
BstAPI GCANNNNNTGC 1 cut(s) 692
BstAUI TGTACA 1 cut(s) 714
BstDEI CTNAG 3 cut(s) 152, 219, 906
BstEII GGTNACC 1 cut(s) 742
BstHHI GCGC 1 cut(s) 725
BstKTI GATC 6 cut(s) 103, 109, 357, 421, 508, 608
BstMBI GATC 6 cut(s) 100, 106, 354, 418, 505, 605
BstMCI CGRYCG 1 cut(s) 109
BstMWI GCNNNNNNNGC 1 cut(s) 692
BstNI CCWGG 1 cut(s) 780
BstPI GGTNACC 1 cut(s) 742
BstSCI CCNGG 1 cut(s) 778
BstSFI CTRYAG 2 cut(s) 430, 551
BstV1I GCAGC 2 cut(s) 737, 740
BstV2I GAAGAC 1 cut(s) 867
BstX2I RGATCY 1 cut(s) 605
BstYI RGATCY 1 cut(s) 605
BstZ17I GTATAC 1 cut(s) 582
BsuI GTATCC 1 cut(s) 45
BsuRI GGCC 2 cut(s) 373, 454
BtsI GCAGTG 1 cut(s) 810
BtsIMutI CAGTG 1 cut(s) 810
CaiI CAGNNNCTG 1 cut(s) 731
CciI TCATGA 1 cut(s) 411
CfoI GCGC 1 cut(s) 725
Cfr13I GGNCC 3 cut(s) 206, 371, 782
CseI GACGC 1 cut(s) 809
CsiI ACCWGGT 1 cut(s) 778
Csp6I GTAC 4 cut(s) 26, 149, 601, 715
CviAII CATG 3 cut(s) 32, 412, 541
CviQI GTAC 4 cut(s) 26, 149, 601, 715
DdeI CTNAG 3 cut(s) 152, 219, 906
DpnI GATC 6 cut(s) 102, 108, 356, 420, 507, 607
DpnII GATC 6 cut(s) 100, 106, 354, 418, 505, 605
Eam1104I CTCTTC 1 cut(s) 664
EarI CTCTTC 1 cut(s) 664
Ecl136II GAGCTC 1 cut(s) 442
Eco130I CCWWGG 2 cut(s) 534, 747
Eco24I GRGCYC 2 cut(s) 237, 444
Eco47I GGWCC 2 cut(s) 206, 782
Eco53kI GAGCTC 1 cut(s) 442
Eco88I CYCGRG 1 cut(s) 18
Eco91I GGTNACC 1 cut(s) 742
EcoICRI GAGCTC 1 cut(s) 442
EcoO65I GGTNACC 1 cut(s) 742
EcoRII CCWGG 1 cut(s) 778
EcoT14I CCWWGG 2 cut(s) 534, 747
EcoT38I GRGCYC 2 cut(s) 237, 444
ErhI CCWWGG 2 cut(s) 534, 747
FaeI CATG 3 cut(s) 35, 415, 544
FaqI GGGAC 1 cut(s) 289
FatI CATG 3 cut(s) 31, 411, 540
FbaI TGATCA 1 cut(s) 354
FblI GTMKAC 2 cut(s) 581, 852
Fnu4HI GCNGC 4 cut(s) 374, 455, 726, 729
FriOI GRGCYC 2 cut(s) 237, 444
Fsp4HI GCNGC 4 cut(s) 374, 455, 726, 729
FspBI CTAG 2 cut(s) 266, 342
GlaI GCGC 1 cut(s) 724
GluI GCNGC 4 cut(s) 374, 455, 726, 729
GsaI CCCAGC 1 cut(s) 173
GsuI CTGGAG 1 cut(s) 431
HaeIII GGCC 2 cut(s) 373, 454
HapII CCGG 1 cut(s) 209
HgaI GACGC 1 cut(s) 809
HhaI GCGC 1 cut(s) 725
Hin1I GRCGYC 1 cut(s) 801
Hin1II CATG 3 cut(s) 35, 415, 544
Hin6I GCGC 1 cut(s) 723
HinP1I GCGC 1 cut(s) 723
HinfI GANTC 2 cut(s) 215, 365
HpaII CCGG 1 cut(s) 209
HphI GGTGA 1 cut(s) 110
Hpy166II GTNNAC 3 cut(s) 582, 715, 853
Hpy188I TCNGA 1 cut(s) 92
Hpy188III TCNNGA 7 cut(s) 104, 362, 412, 422, 448, 497, 572
Hpy8I GTNNAC 3 cut(s) 582, 715, 853
Hpy99I CGWCG 1 cut(s) 803
HpyAV CCTTC 2 cut(s) 377, 418
HpyCH4III ACNGT 6 cut(s) 325, 400, 434, 586, 625, 844
HpyCH4IV ACGT 1 cut(s) 557
HpyCH4V TGCA 4 cut(s) 191, 254, 686, 815
HpyF10VI GCNNNNNNNGC 1 cut(s) 692
HpyF3I CTNAG 3 cut(s) 152, 219, 906
HpySE526I ACGT 1 cut(s) 557
Hsp92I GRCGYC 1 cut(s) 801
Hsp92II CATG 3 cut(s) 35, 415, 544
HspAI GCGC 1 cut(s) 723
Ksp22I TGATCA 1 cut(s) 354
Kzo9I GATC 6 cut(s) 100, 106, 354, 418, 505, 605
LmnI GCTCC 1 cut(s) 447
LpnPI CCDG 8 cut(s) 183, 222, 354, 435, 461, 674, 765, 792
Lsp1109I GCAGC 2 cut(s) 737, 740
MabI ACCWGGT 1 cut(s) 778
MaeI CTAG 2 cut(s) 266, 342
MaeII ACGT 1 cut(s) 557
MaeIII GTNAC 2 cut(s) 655, 742
MalI GATC 6 cut(s) 102, 108, 356, 420, 507, 607
MbiI CCGCTC 2 cut(s) 376, 577
MboI GATC 6 cut(s) 100, 106, 354, 418, 505, 605
MboII GAAGA 4 cut(s) 224, 681, 872, 893
MflI RGATCY 1 cut(s) 605
MhlI GDGCHC 2 cut(s) 237, 444
MluCI AATT 3 cut(s) 131, 646, 876
MmeI TCCRAC 1 cut(s) 736
MnlI CCTC 8 cut(s) 86, 250, 385, 444, 455, 579, 665, 752
MroXI GAANNNNTTC 1 cut(s) 333
MseI TTAA 2 cut(s) 117, 489
MslI CAYNNNNRTG 2 cut(s) 36, 249
MspA1I CMGCKG 1 cut(s) 731
MspI CCGG 1 cut(s) 209
MspR9I CCNGG 1 cut(s) 780
Mva1269I GAATGC 1 cut(s) 193
MvaI CCWGG 1 cut(s) 780
MwoI GCNNNNNNNGC 1 cut(s) 692
NdeII GATC 6 cut(s) 100, 106, 354, 418, 505, 605
NlaIII CATG 3 cut(s) 35, 415, 544
NlaIV GGNNCC 2 cut(s) 397, 607
NmuCI GTSAC 1 cut(s) 655
PagI TCATGA 1 cut(s) 411
PcsI WCGNNNNNNNCGW 1 cut(s) 76
PctI GAATGC 1 cut(s) 193
PdmI GAANNNNTTC 1 cut(s) 333
PfeI GAWTC 2 cut(s) 215, 365
PkrI GCNGC 4 cut(s) 375, 456, 727, 730
Ple19I CGATCG 1 cut(s) 109
PsiI TTATAA 1 cut(s) 651
Psp124BI GAGCTC 1 cut(s) 444
Psp6I CCWGG 1 cut(s) 778
PspEI GGTNACC 1 cut(s) 742
PspFI CCCAGC 1 cut(s) 169
PspGI CCWGG 1 cut(s) 778
PspN4I GGNNCC 2 cut(s) 397, 607
PspPI GGNCC 3 cut(s) 206, 371, 782
PstNI CAGNNNCTG 1 cut(s) 731
PsuI RGATCY 1 cut(s) 605
PvuI CGATCG 1 cut(s) 109
PvuII CAGCTG 1 cut(s) 731
RsaI GTAC 4 cut(s) 27, 150, 602, 716
RsaNI GTAC 4 cut(s) 26, 149, 601, 715
RseI CAYNNNNRTG 2 cut(s) 36, 249
SacI GAGCTC 1 cut(s) 444
SaqAI TTAA 2 cut(s) 117, 489
SatI GCNGC 4 cut(s) 374, 455, 726, 729
Sau3AI GATC 6 cut(s) 100, 106, 354, 418, 505, 605
Sau96I GGNCC 3 cut(s) 206, 371, 782
ScaI AGTACT 1 cut(s) 150
ScrFI CCNGG 1 cut(s) 780
SduI GDGCHC 2 cut(s) 237, 444
SexAI ACCWGGT 1 cut(s) 778
SfcI CTRYAG 2 cut(s) 430, 551
SinI GGWCC 2 cut(s) 206, 782
SmiMI CAYNNNNRTG 2 cut(s) 36, 249
SmlI CTYRAG 1 cut(s) 570
SmoI CTYRAG 1 cut(s) 570
Sse9I AATT 3 cut(s) 131, 646, 876
SsiI CCGC 3 cut(s) 374, 455, 577
SspMI CTAG 2 cut(s) 266, 342
SstI GAGCTC 1 cut(s) 444
StyD4I CCNGG 1 cut(s) 778
StyI CCWWGG 2 cut(s) 534, 747
TaaI ACNGT 6 cut(s) 325, 400, 434, 586, 625, 844
TaiI ACGT 1 cut(s) 560
TaqI TCGA 6 cut(s) 105, 185, 337, 438, 633, 798
TasI AATT 3 cut(s) 131, 646, 876
TatI WGTACW 2 cut(s) 148, 714
TauI GCSGC 2 cut(s) 376, 457
TfiI GAWTC 2 cut(s) 215, 365
Tru1I TTAA 2 cut(s) 117, 489
Tru9I TTAA 2 cut(s) 117, 489
TscAI CASTG 1 cut(s) 817
TseFI GTSAC 1 cut(s) 655
TseI GCWGC 2 cut(s) 725, 728
Tsp45I GTSAC 1 cut(s) 655
TspDTI ATGAA 6 cut(s) 158, 400, 428, 557, 873, 908
TspGWI ACGGA 2 cut(s) 68, 618
TspRI CASTG 1 cut(s) 817
VpaK11BI GGWCC 2 cut(s) 206, 782
XapI RAATTY 1 cut(s) 131
XmiI GTMKAC 2 cut(s) 581, 852
XmnI GAANNNNTTC 1 cut(s) 333
XspI CTAG 2 cut(s) 266, 342
ZrmI AGTACT 1 cut(s) 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.