MD11G1215300.v1.1

lactoylglutathione

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0002543
Physical Location & Seq
Forward (+)
31449069 .. 31451532
2464 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1215300.v1.1.491

Sequence Viewer

Length: 855 bp
ATGGGATCAAGTATGGCAGCTCGGGCAGTACCCCATGACGATGTGTTAGAATGGGTACAGAAAGATAGTCGTCGTTTCCTGCGTGCTAATATCAGAGTTAGTGATCTTGATCGCACCATTAAGTTTTACACACAACTTTTTGGATTGAAAGTATTGAGTAGAAAAGATTTCCCAGAAGAAAAATACTCATATGCGATCGTTGGGTTTGGACCTGAAGAATCTCACTTTGTGATAGGGCTCACATGCCATCACAACACAACGGACAAGCTAGACATCGGAACAGCCTTTAGTCACTTTGGAATTGCAACCCAAGATATTTATAACACGGTTGAAAAAGTTCGAGCCAGTGGTGGTGTGATCACTCGTGAACCCGGGCCCCTCGTAGAAGGAGGGGATACCGTTTACGCCTTCATGAACGATCCCGACGGCTACAGTTTTGAGCTCCTCCAGAGGCCGCCAACTCCAGAACCACTTTCTCATATATGTCTTAATGTTATTGATTTAAATCGGTCTATTGAGTTCTACAACAAGTCCTTGGGCATGAACCTACTACTAAAGTTTGACGTCCCTCAAGAGCAGTATACCGTAGGTATGGTGGGGTATGGATCCAATCTCACTCAAACAGTTGTGATCGAGTTGAAATACAATTATAATGTGACGGCATACAGGAGAGGAAATGGTTACGCACAGGTTGCTATTGGCACTGATGACGTGTACAAGAGCGCAGCAGCTGCAAAACTTGTTACCAAGGAAGTTGGAGGGAAAGTAATAAGACCACCGGGTCCACTCCCAAAAATTCACACCAAGATCACAGCATTCCTTGATACAGATGGCTTTGAAACGGTAAGGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00620 map00620
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

285

Amino Acids

31.64

Weight (kDa)

6.39

Isoelectric Point (pI)

35.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 27 - 148 1.3e-17 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase_4 PF13669 31 - 131 5.1e-06 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase PF00903 158 - 280 1.1e-10 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 321, 651
AasI GACNNNNNNGTC 1 cut(s) 780
AatII GACGTC 1 cut(s) 567
AccI GTMKAC 1 cut(s) 581
AciI CCGC 1 cut(s) 455
AclWI GGATC 4 cut(s) 13, 413, 600, 613
AcsI RAATTY 1 cut(s) 795
AcuI CTGAAG 1 cut(s) 234
AcyI GRCGYC 1 cut(s) 564
AdeI CACNNNGTG 1 cut(s) 229
AfaI GTAC 3 cut(s) 30, 57, 716
AflIII ACRYGT 1 cut(s) 711
AgsI TTSAA 4 cut(s) 148, 332, 640, 839
AjiI CACGTC 1 cut(s) 712
AluBI AGCT 4 cut(s) 20, 268, 442, 731
AluI AGCT 4 cut(s) 20, 268, 442, 731
Alw21I GWGCWC 1 cut(s) 444
AlwI GGATC 4 cut(s) 13, 413, 600, 613
AlwNI CAGNNNCTG 1 cut(s) 731
Ama87I CYCGRG 2 cut(s) 21, 371
AoxI GGCC 2 cut(s) 374, 452
ApaI GGGCCC 1 cut(s) 378
ApeKI GCWGC 4 cut(s) 17, 725, 728, 731
ApoI RAATTY 1 cut(s) 795
Asp700I GAANNNNTTC 1 cut(s) 336
AspLEI GCGC 1 cut(s) 725
AspS9I GGNCC 4 cut(s) 209, 374, 375, 782
AsuC2I CCSGG 3 cut(s) 372, 373, 780
AvaI CYCGRG 2 cut(s) 21, 371
AvaII GGWCC 2 cut(s) 209, 782
BaeGI GKGCMC 1 cut(s) 378
BamHI GGATCC 1 cut(s) 605
BanII GRGCYC 3 cut(s) 240, 378, 444
BauI CACGAG 1 cut(s) 363
Bbv12I GWGCWC 1 cut(s) 444
BbvI GCAGC 4 cut(s) 29, 718, 737, 740
BccI CCATC 2 cut(s) 255, 824
BceAI ACGGC 2 cut(s) 442, 675
BciVI GTATCC 1 cut(s) 388
BclI TGATCA 1 cut(s) 357
BcnI CCSGG 3 cut(s) 372, 373, 780
BfaI CTAG 2 cut(s) 269, 853
BfmI CTRYAG 1 cut(s) 430
BfuI GTATCC 1 cut(s) 388
BisI GCNGC 5 cut(s) 18, 455, 726, 729, 732
BlsI GCNGC 5 cut(s) 19, 456, 727, 730, 733
Bme1390I CCNGG 3 cut(s) 372, 373, 780
Bme18I GGWCC 2 cut(s) 209, 782
BmeT110I CYCGRG 2 cut(s) 21, 371
BmgBI CACGTC 1 cut(s) 712
BmgT120I GGNCC 4 cut(s) 209, 374, 375, 782
BmiI GGNNCC 4 cut(s) 376, 377, 607, 783
BmrFI CCNGG 3 cut(s) 372, 373, 780
BpmI CTGGAG 2 cut(s) 431, 447
BpuEI CTTGAG 1 cut(s) 555
BpuMI CCSGG 3 cut(s) 372, 373, 780
BsaBI GATNNNNATC 2 cut(s) 108, 504
BsaHI GRCGYC 1 cut(s) 564
BsaJI CCNNGG 3 cut(s) 371, 534, 747
BsaXI ACNNNNNCTCC 2 cut(s) 750, 780
Bse1I ACTGG 1 cut(s) 345
Bse8I GATNNNNATC 2 cut(s) 108, 504
BseDI CCNNGG 3 cut(s) 371, 534, 747
BseJI GATNNNNATC 2 cut(s) 108, 504
BseNI ACTGG 1 cut(s) 345
BseRI GAGGAG 1 cut(s) 434
BseSI GKGCMC 1 cut(s) 378
BseXI GCAGC 4 cut(s) 29, 718, 737, 740
Bsh1285I CGRYCG 1 cut(s) 198
BshFI GGCC 2 cut(s) 376, 454
BsiEI CGRYCG 1 cut(s) 198
BsiHKAI GWGCWC 1 cut(s) 444
BsiHKCI CYCGRG 2 cut(s) 21, 371
BsiSI CCGG 2 cut(s) 372, 779
BslFI GGGAC 1 cut(s) 551
BsmFI GGGAC 1 cut(s) 551
BsmI GAATGC 1 cut(s) 815
BsnI GGCC 2 cut(s) 376, 454
BsoBI CYCGRG 2 cut(s) 21, 371
Bsp120I GGGCCC 1 cut(s) 374
Bsp1286I GDGCHC 3 cut(s) 240, 378, 444
Bsp1407I TGTACA 1 cut(s) 714
Bsp143I GATC 9 cut(s) 5, 103, 109, 195, 357, 418, 605, 630, 807
BspACI CCGC 1 cut(s) 455
BspANI GGCC 2 cut(s) 376, 454
BspHI TCATGA 1 cut(s) 411
BspLI GGNNCC 4 cut(s) 376, 377, 607, 783
BspPI GGATC 4 cut(s) 13, 413, 600, 613
BsrGI TGTACA 1 cut(s) 714
BsrI ACTGG 1 cut(s) 345
BssECI CCNNGG 3 cut(s) 371, 534, 747
BssMI GATC 9 cut(s) 5, 103, 109, 195, 357, 418, 605, 630, 807
BssNAI GTATAC 1 cut(s) 582
BssNI GRCGYC 1 cut(s) 564
BssSI CACGAG 1 cut(s) 363
BssT1I CCWWGG 2 cut(s) 534, 747
Bst1107I GTATAC 1 cut(s) 582
Bst2BI CACGAG 1 cut(s) 363
Bst4CI ACNGT 6 cut(s) 328, 400, 434, 586, 625, 844
BstACI GRCGYC 1 cut(s) 564
BstAPI GCANNNNNTGC 2 cut(s) 692, 731
BstAUI TGTACA 1 cut(s) 714
BstC8I GCNNGC 1 cut(s) 84
BstHHI GCGC 1 cut(s) 725
BstKTI GATC 9 cut(s) 8, 106, 112, 198, 360, 421, 608, 633, 810
BstMBI GATC 9 cut(s) 5, 103, 109, 195, 357, 418, 605, 630, 807
BstMCI CGRYCG 1 cut(s) 198
BstMWI GCNNNNNNNGC 3 cut(s) 23, 692, 731
BstNSI RCATGY 1 cut(s) 246
BstSCI CCNGG 3 cut(s) 370, 371, 778
BstSFI CTRYAG 1 cut(s) 430
BstSLI GKGCMC 1 cut(s) 378
BstV1I GCAGC 4 cut(s) 29, 718, 737, 740
BstX2I RGATCY 1 cut(s) 605
BstYI RGATCY 1 cut(s) 605
BstZ17I GTATAC 1 cut(s) 582
BsuI GTATCC 1 cut(s) 388
BsuRI GGCC 2 cut(s) 376, 454
BtrI CACGTC 1 cut(s) 712
BtsIMutI CAGTG 2 cut(s) 352, 702
Cac8I GCNNGC 1 cut(s) 84
CaiI CAGNNNCTG 1 cut(s) 731
CciI TCATGA 1 cut(s) 411
CfoI GCGC 1 cut(s) 725
Cfr13I GGNCC 4 cut(s) 209, 374, 375, 782
Cfr9I CCCGGG 1 cut(s) 371
Csp6I GTAC 3 cut(s) 29, 56, 715
CviAII CATG 4 cut(s) 35, 243, 412, 541
CviQI GTAC 3 cut(s) 29, 56, 715
DpnI GATC 9 cut(s) 7, 105, 111, 197, 359, 420, 607, 632, 809
DpnII GATC 9 cut(s) 5, 103, 109, 195, 357, 418, 605, 630, 807
DraI TTTAAA 1 cut(s) 504
DraIII CACNNNGTG 1 cut(s) 229
DrdI GACNNNNNNGTC 1 cut(s) 780
DseDI GACNNNNNNGTC 1 cut(s) 780
Ecl136II GAGCTC 1 cut(s) 442
Eco130I CCWWGG 2 cut(s) 534, 747
Eco24I GRGCYC 3 cut(s) 240, 378, 444
Eco47I GGWCC 2 cut(s) 209, 782
Eco53kI GAGCTC 1 cut(s) 442
Eco57I CTGAAG 1 cut(s) 234
Eco88I CYCGRG 2 cut(s) 21, 371
EcoICRI GAGCTC 1 cut(s) 442
EcoO109I RGGNCCY 1 cut(s) 375
EcoT14I CCWWGG 2 cut(s) 534, 747
EcoT38I GRGCYC 3 cut(s) 240, 378, 444
ErhI CCWWGG 2 cut(s) 534, 747
FaeI CATG 4 cut(s) 38, 246, 415, 544
FaqI GGGAC 1 cut(s) 551
FatI CATG 4 cut(s) 34, 242, 411, 540
FauNDI CATATG 1 cut(s) 190
FbaI TGATCA 1 cut(s) 357
FblI GTMKAC 1 cut(s) 581
Fnu4HI GCNGC 5 cut(s) 18, 455, 726, 729, 732
FriOI GRGCYC 3 cut(s) 240, 378, 444
Fsp4HI GCNGC 5 cut(s) 18, 455, 726, 729, 732
FspBI CTAG 2 cut(s) 269, 853
GlaI GCGC 1 cut(s) 724
GluI GCNGC 5 cut(s) 18, 455, 726, 729, 732
GsuI CTGGAG 2 cut(s) 431, 447
HaeIII GGCC 2 cut(s) 376, 454
HapII CCGG 2 cut(s) 372, 779
HhaI GCGC 1 cut(s) 725
Hin1I GRCGYC 1 cut(s) 564
Hin1II CATG 4 cut(s) 38, 246, 415, 544
Hin6I GCGC 1 cut(s) 723
HinP1I GCGC 1 cut(s) 723
HinfI GANTC 1 cut(s) 218
HpaII CCGG 2 cut(s) 372, 779
Hpy166II GTNNAC 5 cut(s) 368, 403, 582, 715, 785
Hpy188I TCNGA 2 cut(s) 95, 278
Hpy188III TCNNGA 7 cut(s) 107, 365, 412, 422, 448, 464, 572
Hpy8I GTNNAC 5 cut(s) 368, 403, 582, 715, 785
Hpy99I CGWCG 2 cut(s) 75, 428
HpyAV CCTTC 2 cut(s) 380, 418
HpyCH4III ACNGT 6 cut(s) 328, 400, 434, 586, 625, 844
HpyCH4IV ACGT 2 cut(s) 564, 711
HpyCH4V TGCA 2 cut(s) 305, 734
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 692, 731
HpySE526I ACGT 2 cut(s) 564, 711
Hsp92I GRCGYC 1 cut(s) 564
Hsp92II CATG 4 cut(s) 38, 246, 415, 544
HspAI GCGC 1 cut(s) 723
Ksp22I TGATCA 1 cut(s) 357
Kzo9I GATC 9 cut(s) 5, 103, 109, 195, 357, 418, 605, 630, 807
LmnI GCTCC 1 cut(s) 447
Lsp1109I GCAGC 4 cut(s) 29, 718, 737, 740
MaeI CTAG 2 cut(s) 269, 853
MaeII ACGT 2 cut(s) 564, 711
MaeIII GTNAC 4 cut(s) 290, 655, 680, 742
MalI GATC 9 cut(s) 7, 105, 111, 197, 359, 420, 607, 632, 809
MboI GATC 9 cut(s) 5, 103, 109, 195, 357, 418, 605, 630, 807
MboII GAAGA 2 cut(s) 188, 227
MflI RGATCY 1 cut(s) 605
MhlI GDGCHC 3 cut(s) 240, 378, 444
MluCI AATT 3 cut(s) 300, 646, 795
MmeI TCCRAC 1 cut(s) 736
MnlI CCTC 7 cut(s) 383, 389, 444, 455, 579, 665, 752
MroXI GAANNNNTTC 1 cut(s) 336
MseI TTAA 3 cut(s) 120, 489, 503
MslI CAYNNNNRTG 1 cut(s) 39
MspA1I CMGCKG 1 cut(s) 731
MspI CCGG 2 cut(s) 372, 779
MspR9I CCNGG 3 cut(s) 372, 373, 780
Mva1269I GAATGC 1 cut(s) 815
MwoI GCNNNNNNNGC 3 cut(s) 23, 692, 731
NciI CCSGG 3 cut(s) 372, 373, 780
NdeI CATATG 1 cut(s) 190
NdeII GATC 9 cut(s) 5, 103, 109, 195, 357, 418, 605, 630, 807
NlaIII CATG 4 cut(s) 38, 246, 415, 544
NlaIV GGNNCC 4 cut(s) 376, 377, 607, 783
NmuCI GTSAC 2 cut(s) 290, 655
NspI RCATGY 1 cut(s) 246
PagI TCATGA 1 cut(s) 411
PcsI WCGNNNNNNNCGW 1 cut(s) 79
PctI GAATGC 1 cut(s) 815
PdmI GAANNNNTTC 1 cut(s) 336
PfeI GAWTC 1 cut(s) 218
PkrI GCNGC 5 cut(s) 19, 456, 727, 730, 733
Ple19I CGATCG 1 cut(s) 198
PsiI TTATAA 2 cut(s) 321, 651
Psp124BI GAGCTC 1 cut(s) 444
PspN4I GGNNCC 4 cut(s) 376, 377, 607, 783
PspOMI GGGCCC 1 cut(s) 374
PspPI GGNCC 4 cut(s) 209, 374, 375, 782
PstNI CAGNNNCTG 1 cut(s) 731
PsuI RGATCY 1 cut(s) 605
PvuI CGATCG 1 cut(s) 198
PvuII CAGCTG 1 cut(s) 731
RsaI GTAC 3 cut(s) 30, 57, 716
RsaNI GTAC 3 cut(s) 29, 56, 715
RseI CAYNNNNRTG 1 cut(s) 39
SacI GAGCTC 1 cut(s) 444
SaqAI TTAA 3 cut(s) 120, 489, 503
SatI GCNGC 5 cut(s) 18, 455, 726, 729, 732
Sau3AI GATC 9 cut(s) 5, 103, 109, 195, 357, 418, 605, 630, 807
Sau96I GGNCC 4 cut(s) 209, 374, 375, 782
ScrFI CCNGG 3 cut(s) 372, 373, 780
SduI GDGCHC 3 cut(s) 240, 378, 444
SfcI CTRYAG 1 cut(s) 430
SinI GGWCC 2 cut(s) 209, 782
SmaI CCCGGG 1 cut(s) 373
SmiI ATTTAAAT 1 cut(s) 504
SmiMI CAYNNNNRTG 1 cut(s) 39
SmlI CTYRAG 1 cut(s) 570
SmoI CTYRAG 1 cut(s) 570
Sse9I AATT 3 cut(s) 300, 646, 795
SsiI CCGC 1 cut(s) 455
SspMI CTAG 2 cut(s) 269, 853
SstI GAGCTC 1 cut(s) 444
StyD4I CCNGG 3 cut(s) 370, 371, 778
StyI CCWWGG 2 cut(s) 534, 747
SwaI ATTTAAAT 1 cut(s) 504
TaaI ACNGT 6 cut(s) 328, 400, 434, 586, 625, 844
TaiI ACGT 2 cut(s) 567, 714
TaqI TCGA 2 cut(s) 340, 633
TaqII GACCGA 1 cut(s) 498
TasI AATT 3 cut(s) 300, 646, 795
TatI WGTACW 1 cut(s) 714
TauI GCSGC 1 cut(s) 457
TfiI GAWTC 1 cut(s) 218
Tru1I TTAA 3 cut(s) 120, 489, 503
Tru9I TTAA 3 cut(s) 120, 489, 503
TscAI CASTG 2 cut(s) 352, 709
TseFI GTSAC 2 cut(s) 290, 655
TseI GCWGC 4 cut(s) 17, 725, 728, 731
Tsp45I GTSAC 2 cut(s) 290, 655
TspDTI ATGAA 3 cut(s) 400, 428, 557
TspGWI ACGGA 1 cut(s) 275
TspMI CCCGGG 1 cut(s) 371
TspRI CASTG 2 cut(s) 352, 709
VpaK11BI GGWCC 2 cut(s) 209, 782
XapI RAATTY 1 cut(s) 795
XceI RCATGY 1 cut(s) 246
XmaI CCCGGG 1 cut(s) 371
XmiI GTMKAC 1 cut(s) 581
XmnI GAANNNNTTC 1 cut(s) 336
XspI CTAG 2 cut(s) 269, 853
ZraI GACGTC 1 cut(s) 565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.