Rorug05G0136300

lactoylglutathione

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0002543
Physical Location & Seq
Forward (+)
12277072 .. 12277854
783 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0136300.1

Sequence Viewer

Length: 783 bp
ATGGCCTCATCTGTAACAGACCTCTTAATTGGAAAACTTGCGACGATTCTTGAGAACGAAGGAACAACCATAGCTGGTGTTCGTGATGAAGTTGATAAGGTTAAGGAGGAGCTTGAAAGCATGAAAGCTTTCCTAGTGGATGCAGAGGCCAACAAAGCAAAAATTGAAGGAGAGAAATTGTGGGTTGCAAGAATCAGAGACTTAGCCTATGATGTTGAAGATATCATTGATGAATTCATGTATCACATGTATGAGAAGCAAAGTGGGGGTCGACTTACAAGGGGGCTACACAAAACCATTCGCGCTCCACGCAATCTTTGGTTCAGGCATAAAATTGCAAAGAAGTTGCAAAAAATTACTGAAATGATCAAAGCCAACCCAGAGAGGAATCAAAGATACGGTGTTGGCCTTGTAGGAGGAGCAACTACATCTGAAGATATTCACAAATTGGTGCAGAACCAAGCTGAGTCTTCGTTTTTCATTATGGAAGACGAACTGGTTGGGATTGAAGGCAAGAAGCAAATACTAATGGGATGGCTCATGGATGAAGAGCAACACCAAACTGTTATATCTGTGGTTGGCATGGGAGGTTCTGGAAAGACAACTCTAGTTGCCAAGACCTTCAACAATGAAAGGGTGAAGAGACATTTCGATTGTTATGCATGGATTACCGTTTCCCAAACTTATGATGTGGTACACTTGTTTCGAAGCTTGATCAAGGAACTCTACAAATCAAGGAAGGAAGAGTGCTCTGCAAATTTGAATGCCATGAGCCGCACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00620 map00620
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

260

Amino Acids

29.62

Weight (kDa)

7.07

Isoelectric Point (pI)

46.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 8 - 89 1.2e-24 Rx N-terminal domain
NB-ARC PF00931 172 - 249 2.2e-20 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 271
AccII CGCG 1 cut(s) 303
AciI CCGC 1 cut(s) 775
AcsI RAATTY 2 cut(s) 233, 757
AcuI CTGAAG 1 cut(s) 453
AfaI GTAC 1 cut(s) 696
AflIII ACRYGT 1 cut(s) 246
AgsI TTSAA 6 cut(s) 116, 167, 218, 509, 625, 763
AluBI AGCT 5 cut(s) 74, 112, 128, 464, 711
AluI AGCT 5 cut(s) 74, 112, 128, 464, 711
Alw21I GWGCWC 1 cut(s) 752
Alw26I GTCTC 2 cut(s) 192, 637
AoxI GGCC 3 cut(s) 3, 147, 406
ApoI RAATTY 2 cut(s) 233, 757
ArsI GACNNNNNNTTYG 2 cut(s) 253, 285
Asp700I GAANNNNTTC 2 cut(s) 128, 438
AspLEI GCGC 1 cut(s) 305
AsuHPI GGTGA 1 cut(s) 649
AsuII TTCGAA 1 cut(s) 706
BaeI ACNNNNGTAYC 2 cut(s) 686, 719
BbsI GAAGAC 2 cut(s) 462, 495
Bbv12I GWGCWC 1 cut(s) 752
BccI CCATC 1 cut(s) 528
BcgI CGANNNNNNTGC 2 cut(s) 641, 675
BclI TGATCA 2 cut(s) 366, 714
BcoDI GTCTC 2 cut(s) 192, 637
BfaI CTAG 2 cut(s) 134, 608
BisI GCNGC 1 cut(s) 775
BlsI GCNGC 1 cut(s) 776
BmsI GCATC 1 cut(s) 130
BpiI GAAGAC 2 cut(s) 462, 495
Bpu14I TTCGAA 1 cut(s) 706
BpuEI CTTGAG 1 cut(s) 71
Bse1I ACTGG 1 cut(s) 501
BseGI GGATG 3 cut(s) 145, 539, 550
BseMII CTCAG 1 cut(s) 456
BseNI ACTGG 1 cut(s) 501
BseRI GAGGAG 2 cut(s) 122, 432
BsgI GTGCAG 1 cut(s) 473
Bsh1236I CGCG 1 cut(s) 303
BshFI GGCC 3 cut(s) 5, 149, 408
BsiHKAI GWGCWC 1 cut(s) 752
BsmAI GTCTC 2 cut(s) 192, 637
BsmI GAATGC 1 cut(s) 769
BsnI GGCC 3 cut(s) 5, 149, 408
Bsp119I TTCGAA 1 cut(s) 706
Bsp1286I GDGCHC 1 cut(s) 752
Bsp143I GATC 2 cut(s) 366, 714
BspACI CCGC 1 cut(s) 775
BspANI GGCC 3 cut(s) 5, 149, 408
BspCNI CTCAG 1 cut(s) 457
BspFNI CGCG 1 cut(s) 303
BspQI GCTCTTC 1 cut(s) 543
BspT104I TTCGAA 1 cut(s) 706
BsrI ACTGG 1 cut(s) 501
BssMI GATC 2 cut(s) 366, 714
Bst4CI ACNGT 3 cut(s) 401, 565, 673
Bst6I CTCTTC 3 cut(s) 543, 635, 738
BstBI TTCGAA 1 cut(s) 706
BstDEI CTNAG 2 cut(s) 202, 465
BstF5I GGATG 3 cut(s) 145, 539, 550
BstFNI CGCG 1 cut(s) 303
BstHHI GCGC 1 cut(s) 305
BstKTI GATC 2 cut(s) 369, 717
BstMAI GTCTC 2 cut(s) 192, 637
BstMBI GATC 2 cut(s) 366, 714
BstMWI GCNNNNNNNGC 2 cut(s) 155, 309
BstNSI RCATGY 1 cut(s) 250
BstUI CGCG 1 cut(s) 303
BstV2I GAAGAC 2 cut(s) 462, 495
BsuRI GGCC 3 cut(s) 5, 149, 408
BtsCI GGATG 3 cut(s) 145, 539, 550
CfoI GCGC 1 cut(s) 305
Csp6I GTAC 1 cut(s) 695
CviAII CATG 7 cut(s) 121, 238, 247, 541, 583, 663, 769
CviQI GTAC 1 cut(s) 695
DdeI CTNAG 2 cut(s) 202, 465
DpnI GATC 2 cut(s) 368, 716
DpnII GATC 2 cut(s) 366, 714
Eam1104I CTCTTC 3 cut(s) 543, 635, 738
EarI CTCTTC 3 cut(s) 543, 635, 738
Eco32I GATATC 1 cut(s) 223
Eco57I CTGAAG 1 cut(s) 453
EcoRI GAATTC 1 cut(s) 233
EcoRV GATATC 1 cut(s) 223
EcoT22I ATGCAT 1 cut(s) 664
FaeI CATG 7 cut(s) 124, 241, 250, 544, 586, 666, 772
FatI CATG 7 cut(s) 120, 237, 246, 540, 582, 662, 768
FbaI TGATCA 2 cut(s) 366, 714
FblI GTMKAC 1 cut(s) 271
Fnu4HI GCNGC 1 cut(s) 775
FokI GGATG 3 cut(s) 152, 546, 557
Fsp4HI GCNGC 1 cut(s) 775
FspBI CTAG 2 cut(s) 134, 608
GlaI GCGC 1 cut(s) 304
GluI GCNGC 1 cut(s) 775
HaeIII GGCC 3 cut(s) 5, 149, 408
HhaI GCGC 1 cut(s) 305
Hin1II CATG 7 cut(s) 124, 241, 250, 544, 586, 666, 772
Hin6I GCGC 1 cut(s) 303
HinP1I GCGC 1 cut(s) 303
HincII GTYRAC 1 cut(s) 272
HindII GTYRAC 1 cut(s) 272
HindIII AAGCTT 2 cut(s) 126, 709
HinfI GANTC 4 cut(s) 46, 192, 388, 467
HphI GGTGA 1 cut(s) 649
Hpy166II GTNNAC 2 cut(s) 272, 697
Hpy188I TCNGA 2 cut(s) 197, 433
Hpy188III TCNNGA 3 cut(s) 50, 83, 594
Hpy8I GTNNAC 2 cut(s) 272, 697
Hpy99I CGWCG 1 cut(s) 46
HpyAV CCTTC 5 cut(s) 53, 161, 503, 631, 733
HpyCH4III ACNGT 3 cut(s) 401, 565, 673
HpyCH4V TGCA 7 cut(s) 143, 188, 338, 349, 454, 662, 755
HpyF10VI GCNNNNNNNGC 2 cut(s) 155, 309
HpyF3I CTNAG 2 cut(s) 202, 465
Hsp92II CATG 7 cut(s) 124, 241, 250, 544, 586, 666, 772
HspAI GCGC 1 cut(s) 303
Ksp22I TGATCA 2 cut(s) 366, 714
Kzo9I GATC 2 cut(s) 366, 714
LguI GCTCTTC 1 cut(s) 543
LmnI GCTCC 3 cut(s) 109, 310, 419
LpnPI CCDG 5 cut(s) 60, 310, 393, 482, 579
LweI GCATC 1 cut(s) 130
MaeI CTAG 2 cut(s) 134, 608
MaeIII GTNAC 1 cut(s) 13
MalI GATC 2 cut(s) 368, 716
MboI GATC 2 cut(s) 366, 714
MboII GAAGA 7 cut(s) 230, 446, 462, 500, 560, 652, 755
MhlI GDGCHC 1 cut(s) 752
MluCI AATT 8 cut(s) 27, 162, 176, 233, 333, 354, 446, 757
MlyI GAGTC 1 cut(s) 476
MnlI CCTC 7 cut(s) 16, 32, 100, 139, 378, 410, 581
Mph1103I ATGCAT 1 cut(s) 664
MroXI GAANNNNTTC 2 cut(s) 128, 438
MseI TTAA 2 cut(s) 26, 102
MslI CAYNNNNRTG 1 cut(s) 249
Mva1269I GAATGC 1 cut(s) 769
MvnI CGCG 1 cut(s) 303
MwoI GCNNNNNNNGC 2 cut(s) 155, 309
NdeII GATC 2 cut(s) 366, 714
NlaIII CATG 7 cut(s) 124, 241, 250, 544, 586, 666, 772
NsiI ATGCAT 1 cut(s) 664
NspI RCATGY 1 cut(s) 250
NspV TTCGAA 1 cut(s) 706
PciI ACATGT 1 cut(s) 246
PciSI GCTCTTC 1 cut(s) 543
PctI GAATGC 1 cut(s) 769
PdmI GAANNNNTTC 2 cut(s) 128, 438
PfeI GAWTC 3 cut(s) 46, 192, 388
PkrI GCNGC 1 cut(s) 776
PleI GAGTC 1 cut(s) 475
PpsI GAGTC 1 cut(s) 475
PscI ACATGT 1 cut(s) 246
RsaI GTAC 1 cut(s) 696
RsaNI GTAC 1 cut(s) 695
RseI CAYNNNNRTG 1 cut(s) 249
SalI GTCGAC 1 cut(s) 270
SapI GCTCTTC 1 cut(s) 543
SaqAI TTAA 2 cut(s) 26, 102
SatI GCNGC 1 cut(s) 775
Sau3AI GATC 2 cut(s) 366, 714
SchI GAGTC 1 cut(s) 476
SduI GDGCHC 1 cut(s) 752
SetI ASST 9 cut(s) 24, 76, 102, 114, 130, 466, 592, 623, 713
SfaNI GCATC 1 cut(s) 130
SfuI TTCGAA 1 cut(s) 706
SmiMI CAYNNNNRTG 1 cut(s) 249
SmlI CTYRAG 1 cut(s) 50
SmoI CTYRAG 1 cut(s) 50
Sse9I AATT 8 cut(s) 27, 162, 176, 233, 333, 354, 446, 757
SsiI CCGC 1 cut(s) 775
SspMI CTAG 2 cut(s) 134, 608
TaaI ACNGT 3 cut(s) 401, 565, 673
TaqI TCGA 3 cut(s) 271, 651, 706
TasI AATT 8 cut(s) 27, 162, 176, 233, 333, 354, 446, 757
TauI GCSGC 1 cut(s) 777
TfiI GAWTC 3 cut(s) 46, 192, 388
Tru1I TTAA 2 cut(s) 26, 102
Tru9I TTAA 2 cut(s) 26, 102
TspDTI ATGAA 7 cut(s) 102, 137, 226, 246, 469, 561, 645
XapI RAATTY 2 cut(s) 233, 757
XceI RCATGY 1 cut(s) 250
XcmI CCANNNNNNNNNTGG 1 cut(s) 315
XmiI GTMKAC 1 cut(s) 271
XmnI GAANNNNTTC 2 cut(s) 128, 438
XspI CTAG 2 cut(s) 134, 608
Zsp2I ATGCAT 1 cut(s) 664
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.