RchiOBHm_Chr5g0040331

lactoylglutathione

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002543
Physical Location & Seq
Forward (+)
35008257 .. 35012836
4580 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31884

Sequence Viewer

Length: 735 bp
ATGAAGATGGTGAAGAACCAGCAGTTGGTCTACAGCTGTATTCTTTTGTTCATCTGTTTGATTGATTTAAGCAATGCAGCTCGCAGTAATCCCCCTGACGATGTTTTGGAATGGGTTCAAAAAGATGATCGCCGTTTCCTGCGTGCCAATATCAGAATTTATACAATGGTTGAAAAAATTCGAGCTGCTGGTGGGGTGATCACTCGTGAACCAGGGCCAGTTGCACCCGGCGGGACTACCATCTACGCCTTTGTGAACGATCCCGATGGCTACAGTTTCGAGCTCTTCCGCAGGCCGCCAACTCCAGAACCACTTAATCATATATGTCTTAACGTGGTTGACATTGATCGTTCTATTGACTTCTACCAAAAGGCTCTGGGAATGAACTTACTAGTGCCAAAGTACAACAATGCTCAAGAGCAGTATACTGTAGCTATGGTGGGCTATGGATCCAACTATACTCAGACGACCGTCATCGAGCTGCAATATAACTATAATGTGACAGATTATACCAAAGGAAATGGATATGCACAGGTAGCTATTGGCACCGATGATGTATACAAGAGTGCAGCAGTTGTTGAACAGGTTATCCAGGAACTTGGTGGGAAAATAATTCAACCACCAGGTCCAATTCCATTTATCAAGACCAAAATCACTGCATTCCTTGATTCAGATGGCTTTGAAACGGTGTTGGTGGACAATCAAGATTATCTTAAATATCTAAAGGAAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00620 map00620
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

244

Amino Acids

27.48

Weight (kDa)

4.96

Isoelectric Point (pI)

37.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 105 - 228 1e-12 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 545
AccB7I CCANNNNNTGG 1 cut(s) 25
AccI GTMKAC 3 cut(s) 30, 425, 558
AciI CCGC 3 cut(s) 231, 289, 296
AclWI GGATC 3 cut(s) 254, 444, 457
AcsI RAATTY 2 cut(s) 156, 177
AfaI GTAC 1 cut(s) 404
AfiI CCNNNNNNNGG 1 cut(s) 25
AgsI TTSAA 5 cut(s) 119, 173, 581, 617, 683
AhlI ACTAGT 1 cut(s) 391
AjnI CCWGG 3 cut(s) 211, 591, 622
AjuI GAANNNNNNNTTGG 2 cut(s) 8, 40
AloI GAACNNNNNNTCC 2 cut(s) 573, 605
AluBI AGCT 7 cut(s) 36, 80, 185, 283, 434, 481, 539
AluI AGCT 7 cut(s) 36, 80, 185, 283, 434, 481, 539
Alw21I GWGCWC 1 cut(s) 285
AlwI GGATC 3 cut(s) 254, 444, 457
AoxI GGCC 2 cut(s) 215, 293
ApeKI GCWGC 4 cut(s) 77, 185, 481, 569
ApoI RAATTY 2 cut(s) 156, 177
Asp700I GAANNNNTTC 2 cut(s) 114, 177
AspS9I GGNCC 2 cut(s) 215, 626
AsuC2I CCSGG 1 cut(s) 228
AsuHPI GGTGA 2 cut(s) 22, 208
AvaII GGWCC 1 cut(s) 626
BamHI GGATCC 1 cut(s) 449
BanI GGYRCC 1 cut(s) 545
BanII GRGCYC 1 cut(s) 285
BauI CACGAG 1 cut(s) 204
Bbv12I GWGCWC 1 cut(s) 285
BbvI GCAGC 4 cut(s) 89, 172, 468, 581
BccI CCATC 3 cut(s) 248, 260, 668
BceAI ACGGC 1 cut(s) 117
BciT130I CCWGG 3 cut(s) 213, 593, 624
BclI TGATCA 1 cut(s) 198
BcnI CCSGG 1 cut(s) 228
BcuI ACTAGT 1 cut(s) 391
BfaI CTAG 1 cut(s) 392
BfmI CTRYAG 3 cut(s) 31, 271, 429
BisI GCNGC 5 cut(s) 78, 186, 296, 482, 570
BlsI GCNGC 5 cut(s) 79, 187, 297, 483, 571
Bme1390I CCNGG 4 cut(s) 213, 228, 593, 624
Bme18I GGWCC 1 cut(s) 626
BmgT120I GGNCC 2 cut(s) 215, 626
BmiI GGNNCC 2 cut(s) 451, 547
BmrFI CCNGG 4 cut(s) 213, 228, 593, 624
BoxI GACNNNNGTC 1 cut(s) 470
BpmI CTGGAG 1 cut(s) 288
BpuEI CTTGAG 1 cut(s) 399
BpuMI CCSGG 1 cut(s) 228
BsaJI CCNNGG 1 cut(s) 212
Bsc4I CCNNNNNNNGG 1 cut(s) 25
Bse1I ACTGG 1 cut(s) 218
Bse3DI GCAATG 1 cut(s) 79
BseBI CCWGG 3 cut(s) 213, 593, 624
BseDI CCNNGG 1 cut(s) 212
BseLI CCNNNNNNNGG 1 cut(s) 25
BseMI GCAATG 1 cut(s) 79
BseMII CTCAG 1 cut(s) 476
BseNI ACTGG 1 cut(s) 218
BseXI GCAGC 4 cut(s) 89, 172, 468, 581
BsgI GTGCAG 1 cut(s) 588
Bsh1285I CGRYCG 1 cut(s) 471
BshFI GGCC 2 cut(s) 217, 295
BshNI GGYRCC 1 cut(s) 545
BsiEI CGRYCG 1 cut(s) 471
BsiHKAI GWGCWC 1 cut(s) 285
BsiSI CCGG 1 cut(s) 228
BslFI GGGAC 1 cut(s) 247
BslI CCNNNNNNNGG 1 cut(s) 25
BsmFI GGGAC 1 cut(s) 247
BsmI GAATGC 1 cut(s) 659
BsnI GGCC 2 cut(s) 217, 295
Bsp1286I GDGCHC 1 cut(s) 285
Bsp143I GATC 5 cut(s) 127, 198, 259, 346, 449
BspACI CCGC 3 cut(s) 231, 289, 296
BspANI GGCC 2 cut(s) 217, 295
BspCNI CTCAG 1 cut(s) 475
BspLI GGNNCC 2 cut(s) 451, 547
BspPI GGATC 3 cut(s) 254, 444, 457
BspQI GCTCTTC 1 cut(s) 290
BspT107I GGYRCC 1 cut(s) 545
BsrDI GCAATG 1 cut(s) 79
BsrI ACTGG 1 cut(s) 218
BssECI CCNNGG 1 cut(s) 212
BssMI GATC 5 cut(s) 127, 198, 259, 346, 449
BssNAI GTATAC 2 cut(s) 426, 559
BssSI CACGAG 1 cut(s) 204
Bst1107I GTATAC 2 cut(s) 426, 559
Bst2BI CACGAG 1 cut(s) 204
Bst2UI CCWGG 3 cut(s) 213, 593, 624
Bst4CI ACNGT 4 cut(s) 275, 430, 472, 688
Bst6I CTCTTC 2 cut(s) 290, 723
BstC8I GCNNGC 3 cut(s) 82, 144, 293
BstDEI CTNAG 1 cut(s) 462
BstKTI GATC 5 cut(s) 130, 201, 262, 349, 452
BstMBI GATC 5 cut(s) 127, 198, 259, 346, 449
BstMCI CGRYCG 1 cut(s) 471
BstMWI GCNNNNNNNGC 1 cut(s) 536
BstNI CCWGG 3 cut(s) 213, 593, 624
BstPAI GACNNNNGTC 1 cut(s) 470
BstSCI CCNGG 4 cut(s) 211, 226, 591, 622
BstSFI CTRYAG 3 cut(s) 31, 271, 429
BstV1I GCAGC 4 cut(s) 89, 172, 468, 581
BstX2I RGATCY 1 cut(s) 449
BstXI CCANNNNNNTGG 1 cut(s) 599
BstYI RGATCY 1 cut(s) 449
BstZ17I GTATAC 2 cut(s) 426, 559
BsuRI GGCC 2 cut(s) 217, 295
BtsI GCAGTG 1 cut(s) 654
BtsIMutI CAGTG 1 cut(s) 654
Cac8I GCNNGC 3 cut(s) 82, 144, 293
Cfr13I GGNCC 2 cut(s) 215, 626
CsiI ACCWGGT 1 cut(s) 622
Csp6I GTAC 1 cut(s) 403
CviQI GTAC 1 cut(s) 403
DdeI CTNAG 1 cut(s) 462
DpnI GATC 5 cut(s) 129, 200, 261, 348, 451
DpnII GATC 5 cut(s) 127, 198, 259, 346, 449
Eam1104I CTCTTC 2 cut(s) 290, 723
EarI CTCTTC 2 cut(s) 290, 723
Ecl136II GAGCTC 1 cut(s) 283
Eco24I GRGCYC 1 cut(s) 285
Eco47I GGWCC 1 cut(s) 626
Eco53kI GAGCTC 1 cut(s) 283
EcoICRI GAGCTC 1 cut(s) 283
EcoRII CCWGG 3 cut(s) 211, 591, 622
EcoT38I GRGCYC 1 cut(s) 285
FalI AAGNNNNNCTT 2 cut(s) 696, 728
FaqI GGGAC 1 cut(s) 247
FauI CCCGC 1 cut(s) 224
FbaI TGATCA 1 cut(s) 198
FblI GTMKAC 3 cut(s) 30, 425, 558
Fnu4HI GCNGC 5 cut(s) 78, 186, 296, 482, 570
FriOI GRGCYC 1 cut(s) 285
Fsp4HI GCNGC 5 cut(s) 78, 186, 296, 482, 570
FspBI CTAG 1 cut(s) 392
GluI GCNGC 5 cut(s) 78, 186, 296, 482, 570
GsuI CTGGAG 1 cut(s) 288
HaeIII GGCC 2 cut(s) 217, 295
HapII CCGG 1 cut(s) 228
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HinfI GANTC 1 cut(s) 668
HpaII CCGG 1 cut(s) 228
HphI GGTGA 2 cut(s) 22, 208
Hpy166II GTNNAC 7 cut(s) 31, 209, 256, 340, 426, 559, 697
Hpy188I TCNGA 3 cut(s) 155, 465, 673
Hpy188III TCNNGA 6 cut(s) 206, 263, 305, 416, 643, 704
Hpy8I GTNNAC 7 cut(s) 31, 209, 256, 340, 426, 559, 697
HpyCH4III ACNGT 4 cut(s) 275, 430, 472, 688
HpyCH4IV ACGT 1 cut(s) 333
HpyCH4V TGCA 6 cut(s) 77, 224, 484, 530, 569, 659
HpyF10VI GCNNNNNNNGC 1 cut(s) 536
HpyF3I CTNAG 1 cut(s) 462
HpySE526I ACGT 1 cut(s) 333
Ksp22I TGATCA 1 cut(s) 198
Kzo9I GATC 5 cut(s) 127, 198, 259, 346, 449
LguI GCTCTTC 1 cut(s) 290
Lsp1109I GCAGC 4 cut(s) 89, 172, 468, 581
MabI ACCWGGT 1 cut(s) 622
MaeI CTAG 1 cut(s) 392
MaeII ACGT 1 cut(s) 333
MaeIII GTNAC 1 cut(s) 499
MalI GATC 5 cut(s) 129, 200, 261, 348, 451
MboI GATC 5 cut(s) 127, 198, 259, 346, 449
MboII GAAGA 3 cut(s) 16, 25, 277
MflI RGATCY 1 cut(s) 449
MhlI GDGCHC 1 cut(s) 285
MluCI AATT 4 cut(s) 156, 177, 612, 630
MmeI TCCRAC 1 cut(s) 477
MroXI GAANNNNTTC 2 cut(s) 114, 177
MseI TTAA 4 cut(s) 68, 315, 330, 714
MspA1I CMGCKG 1 cut(s) 36
MspI CCGG 1 cut(s) 228
MspR9I CCNGG 4 cut(s) 213, 228, 593, 624
Mva1269I GAATGC 1 cut(s) 659
MvaI CCWGG 3 cut(s) 213, 593, 624
MwoI GCNNNNNNNGC 1 cut(s) 536
NciI CCSGG 1 cut(s) 228
NdeII GATC 5 cut(s) 127, 198, 259, 346, 449
NlaIV GGNNCC 2 cut(s) 451, 547
NmuCI GTSAC 1 cut(s) 499
PciSI GCTCTTC 1 cut(s) 290
PctI GAATGC 1 cut(s) 659
PdmI GAANNNNTTC 2 cut(s) 114, 177
PfeI GAWTC 1 cut(s) 668
PflMI CCANNNNNTGG 1 cut(s) 25
PfoI TCCNGGA 1 cut(s) 591
PkrI GCNGC 5 cut(s) 79, 187, 297, 483, 571
PshAI GACNNNNGTC 1 cut(s) 470
Psp124BI GAGCTC 1 cut(s) 285
Psp6I CCWGG 3 cut(s) 211, 591, 622
PspGI CCWGG 3 cut(s) 211, 591, 622
PspN4I GGNNCC 2 cut(s) 451, 547
PspPI GGNCC 2 cut(s) 215, 626
PsuI RGATCY 1 cut(s) 449
PvuII CAGCTG 1 cut(s) 36
RsaI GTAC 1 cut(s) 404
RsaNI GTAC 1 cut(s) 403
SacI GAGCTC 1 cut(s) 285
SapI GCTCTTC 1 cut(s) 290
SaqAI TTAA 4 cut(s) 68, 315, 330, 714
SatI GCNGC 5 cut(s) 78, 186, 296, 482, 570
Sau3AI GATC 5 cut(s) 127, 198, 259, 346, 449
Sau96I GGNCC 2 cut(s) 215, 626
ScrFI CCNGG 4 cut(s) 213, 228, 593, 624
SduI GDGCHC 1 cut(s) 285
SexAI ACCWGGT 1 cut(s) 622
SfcI CTRYAG 3 cut(s) 31, 271, 429
SinI GGWCC 1 cut(s) 626
SmlI CTYRAG 1 cut(s) 414
SmoI CTYRAG 1 cut(s) 414
SpeI ACTAGT 1 cut(s) 391
Sse9I AATT 4 cut(s) 156, 177, 612, 630
SsiI CCGC 3 cut(s) 231, 289, 296
SspMI CTAG 1 cut(s) 392
SstI GAGCTC 1 cut(s) 285
StyD4I CCNGG 4 cut(s) 211, 226, 591, 622
TaaI ACNGT 4 cut(s) 275, 430, 472, 688
TaiI ACGT 1 cut(s) 336
TaqI TCGA 3 cut(s) 181, 279, 477
TasI AATT 4 cut(s) 156, 177, 612, 630
TatI WGTACW 1 cut(s) 402
TauI GCSGC 1 cut(s) 298
TfiI GAWTC 1 cut(s) 668
Tru1I TTAA 4 cut(s) 68, 315, 330, 714
Tru9I TTAA 4 cut(s) 68, 315, 330, 714
TscAI CASTG 1 cut(s) 661
TseFI GTSAC 1 cut(s) 499
TseI GCWGC 4 cut(s) 77, 185, 481, 569
Tsp45I GTSAC 1 cut(s) 499
TspDTI ATGAA 3 cut(s) 17, 40, 398
TspRI CASTG 1 cut(s) 661
Van91I CCANNNNNTGG 1 cut(s) 25
VpaK11BI GGWCC 1 cut(s) 626
XapI RAATTY 2 cut(s) 156, 177
XcmI CCANNNNNNNNNTGG 1 cut(s) 599
XmiI GTMKAC 3 cut(s) 30, 425, 558
XmnI GAANNNNTTC 2 cut(s) 114, 177
XspI CTAG 1 cut(s) 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.