Rw5G020900

lactoylglutathione

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0002543
Physical Location & Seq
Reverse (-)
27721561 .. 27723725
2165 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G020900.1

Sequence Viewer

Length: 927 bp
ATGAAAACAGAAATGTGTTTCAATTTTGACCGATACATGGCAGCTCCAAATAATCCCCATGACGATGTGTTGGAATGGGTGCAAAAAGATAATCGTCGTTTCCTACGTGCCAATATCCGAGTTGGTGATCTTGATCGAACTATTAAGTTTTACACAGAATTTTTTGGGATGAAAGTGTTGAGCAGGCAAGATTTTCCAGAACAAAAATACTCAACTGCAGTCGTTGCGTATGGACCCGAAGAGACTCATTTTGTTCTAGGGCTCACACACCACCACGATGCAGCGGACAAGTTAGACATCGGAACAGCCTTCAATCATTTTGGACTTGCAACCCAAGATGTTTATGGCACAGTTGAAAAAATCCGAGCGGCAGGTGGAGTGATCACACGTGAACCAGGGCCAATTGCAGAGGGTGGGAGGACCATCTTTGCCTTCGTGAACGATCCCGACGGCTACAGTTTTGAGCTCATCCTAAGGCCCCCAACTCCAGAACCACTGTGTCAAATATGTCTTAATGTGACTGATATTGATCGTTCAATTGAATTCTACCAAAAGGCATTGGGAATGAACATACTATTGCCAAAATACGACGCTTCTCAGTTCCAGTATACTATAGCGATGGTGGGTTATGGTTCCGATTTTACTCAGACGACGATTATCGAGTTGAAATATAACTATAATGTGACAGAGTATACCAAAGGAAATGGATATGCAATGCTTGCTATCGGCACTGATGATGTATACAAGAGCGGAGCAGCTGTTGAACTGGTTAACAAGGAACTTGGAGGGGAAATAATTCGACCCCCGGCTGTAAATCCCAAAACCAACACCAAAATCACCTGCTTCCTTGATTCTGATGGGTTTGAATCGGTATTGGTGGACAATCAAGACTATGTTGGCTTTCTTGGAAAATCAAAGGATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00620 map00620
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

308

Amino Acids

34.59

Weight (kDa)

4.96

Isoelectric Point (pI)

31.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 35 - 156 3.1e-20 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase_4 PF13669 39 - 140 2.3e-07 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase PF00903 168 - 289 5.4e-09 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 362, 848
Acc36I ACCTGC 2 cut(s) 362, 848
AccBSI CCGCTC 2 cut(s) 368, 750
AccI GTMKAC 3 cut(s) 608, 692, 741
AciI CCGC 3 cut(s) 284, 368, 750
AclWI GGATC 1 cut(s) 437
AcsI RAATTY 2 cut(s) 158, 542
AcvI CACGTG 1 cut(s) 389
AfiI CCNNNNNNNGG 1 cut(s) 37
AflIII ACRYGT 1 cut(s) 386
AgsI TTSAA 8 cut(s) 22, 313, 356, 537, 542, 667, 764, 866
AjnI CCWGG 1 cut(s) 394
AluBI AGCT 3 cut(s) 44, 466, 758
AluI AGCT 3 cut(s) 44, 466, 758
Alw21I GWGCWC 1 cut(s) 468
Alw26I GTCTC 1 cut(s) 236
AlwI GGATC 1 cut(s) 437
AoxI GGCC 2 cut(s) 398, 476
ApeKI GCWGC 3 cut(s) 41, 281, 755
ApoI RAATTY 2 cut(s) 158, 542
Asp700I GAANNNNTTC 1 cut(s) 795
AspS9I GGNCC 4 cut(s) 233, 398, 420, 477
AsuC2I CCSGG 1 cut(s) 806
AsuHPI GGTGA 2 cut(s) 137, 829
AvaII GGWCC 2 cut(s) 233, 420
AxyI CCTNAGG 1 cut(s) 473
BanII GRGCYC 2 cut(s) 264, 468
BbrPI CACGTG 1 cut(s) 389
Bbv12I GWGCWC 1 cut(s) 468
BbvI GCAGC 3 cut(s) 53, 293, 767
BccI CCATC 3 cut(s) 431, 613, 851
BceAI ACGGC 1 cut(s) 466
BciT130I CCWGG 1 cut(s) 396
BclI TGATCA 1 cut(s) 381
BcnI CCSGG 1 cut(s) 806
BcoDI GTCTC 1 cut(s) 236
BfaI CTAG 1 cut(s) 257
BfmI CTRYAG 3 cut(s) 216, 454, 612
BfuAI ACCTGC 2 cut(s) 362, 848
BisI GCNGC 4 cut(s) 42, 282, 369, 756
BlsI GCNGC 4 cut(s) 43, 283, 370, 757
Bme1390I CCNGG 2 cut(s) 396, 806
Bme18I GGWCC 2 cut(s) 233, 420
BmgT120I GGNCC 4 cut(s) 233, 398, 420, 477
BmiI GGNNCC 3 cut(s) 235, 479, 634
BmrFI CCNGG 2 cut(s) 396, 806
BmsI GCATC 1 cut(s) 268
BpmI CTGGAG 1 cut(s) 471
BpuMI CCSGG 1 cut(s) 806
BsaAI YACGTR 2 cut(s) 107, 389
BsaBI GATNNNNATC 2 cut(s) 132, 528
BsaJI CCNNGG 2 cut(s) 395, 804
BsaXI ACNNNNNCTCC 2 cut(s) 744, 774
Bsc4I CCNNNNNNNGG 1 cut(s) 37
Bse1I ACTGG 2 cut(s) 604, 771
Bse21I CCTNAGG 1 cut(s) 473
Bse3DI GCAATG 1 cut(s) 720
Bse8I GATNNNNATC 2 cut(s) 132, 528
BseBI CCWGG 1 cut(s) 396
BseDI CCNNGG 2 cut(s) 395, 804
BseGI GGATG 3 cut(s) 174, 468, 925
BseJI GATNNNNATC 2 cut(s) 132, 528
BseLI CCNNNNNNNGG 1 cut(s) 37
BseMI GCAATG 1 cut(s) 720
BseMII CTCAG 2 cut(s) 611, 659
BseNI ACTGG 2 cut(s) 604, 771
BseXI GCAGC 3 cut(s) 53, 293, 767
BshFI GGCC 2 cut(s) 400, 478
BsiHKAI GWGCWC 1 cut(s) 468
BsiSI CCGG 1 cut(s) 806
BslI CCNNNNNNNGG 1 cut(s) 37
BsmAI GTCTC 1 cut(s) 236
BsnI GGCC 2 cut(s) 400, 478
Bsp1286I GDGCHC 2 cut(s) 264, 468
Bsp143I GATC 5 cut(s) 127, 133, 381, 442, 529
BspACI CCGC 3 cut(s) 284, 368, 750
BspANI GGCC 2 cut(s) 400, 478
BspCNI CTCAG 2 cut(s) 610, 658
BspLI GGNNCC 3 cut(s) 235, 479, 634
BspMAI CTGCAG 1 cut(s) 220
BspMI ACCTGC 2 cut(s) 362, 848
BspPI GGATC 1 cut(s) 437
BsrBI CCGCTC 2 cut(s) 368, 750
BsrDI GCAATG 1 cut(s) 720
BsrI ACTGG 2 cut(s) 604, 771
BssECI CCNNGG 2 cut(s) 395, 804
BssMI GATC 5 cut(s) 127, 133, 381, 442, 529
BssNAI GTATAC 3 cut(s) 609, 693, 742
Bst1107I GTATAC 3 cut(s) 609, 693, 742
Bst2UI CCWGG 1 cut(s) 396
Bst4CI ACNGT 3 cut(s) 352, 458, 498
Bst6I CTCTTC 1 cut(s) 234
BstAPI GCANNNNNTGC 2 cut(s) 224, 719
BstBAI YACGTR 2 cut(s) 107, 389
BstC8I GCNNGC 2 cut(s) 185, 720
BstDEI CTNAG 3 cut(s) 473, 597, 645
BstF5I GGATG 3 cut(s) 174, 468, 925
BstKTI GATC 5 cut(s) 130, 136, 384, 445, 532
BstMAI GTCTC 1 cut(s) 236
BstMBI GATC 5 cut(s) 127, 133, 381, 442, 529
BstMWI GCNNNNNNNGC 2 cut(s) 224, 719
BstNI CCWGG 1 cut(s) 396
BstSCI CCNGG 2 cut(s) 394, 804
BstSFI CTRYAG 3 cut(s) 216, 454, 612
BstV1I GCAGC 3 cut(s) 53, 293, 767
BstZ17I GTATAC 3 cut(s) 609, 693, 742
Bsu36I CCTNAGG 1 cut(s) 473
BsuRI GGCC 2 cut(s) 400, 478
BtgZI GCGATG 1 cut(s) 632
BtsCI GGATG 3 cut(s) 174, 468, 925
BtsIMutI CAGTG 2 cut(s) 494, 729
BveI ACCTGC 2 cut(s) 362, 848
Cac8I GCNNGC 2 cut(s) 185, 720
Cfr13I GGNCC 4 cut(s) 233, 398, 420, 477
CseI GACGC 1 cut(s) 599
CviAII CATG 2 cut(s) 37, 59
DdeI CTNAG 3 cut(s) 473, 597, 645
DpnI GATC 5 cut(s) 129, 135, 383, 444, 531
DpnII GATC 5 cut(s) 127, 133, 381, 442, 529
Eam1104I CTCTTC 1 cut(s) 234
EarI CTCTTC 1 cut(s) 234
Ecl136II GAGCTC 1 cut(s) 466
Eco24I GRGCYC 2 cut(s) 264, 468
Eco47I GGWCC 2 cut(s) 233, 420
Eco53kI GAGCTC 1 cut(s) 466
Eco72I CACGTG 1 cut(s) 389
Eco81I CCTNAGG 1 cut(s) 473
EcoICRI GAGCTC 1 cut(s) 466
EcoO109I RGGNCCY 1 cut(s) 477
EcoRI GAATTC 1 cut(s) 542
EcoRII CCWGG 1 cut(s) 394
EcoT38I GRGCYC 2 cut(s) 264, 468
FaeI CATG 2 cut(s) 40, 62
FatI CATG 2 cut(s) 36, 58
FbaI TGATCA 1 cut(s) 381
FblI GTMKAC 3 cut(s) 608, 692, 741
Fnu4HI GCNGC 4 cut(s) 42, 282, 369, 756
FokI GGATG 2 cut(s) 181, 455
FriOI GRGCYC 2 cut(s) 264, 468
Fsp4HI GCNGC 4 cut(s) 42, 282, 369, 756
FspBI CTAG 1 cut(s) 257
GluI GCNGC 4 cut(s) 42, 282, 369, 756
GsuI CTGGAG 1 cut(s) 471
HaeIII GGCC 2 cut(s) 400, 478
HapII CCGG 1 cut(s) 806
HgaI GACGC 1 cut(s) 599
Hin1II CATG 2 cut(s) 40, 62
HincII GTYRAC 1 cut(s) 772
HindII GTYRAC 1 cut(s) 772
HinfI GANTC 3 cut(s) 244, 851, 866
HpaI GTTAAC 1 cut(s) 772
HpaII CCGG 1 cut(s) 806
HphI GGTGA 2 cut(s) 137, 829
Hpy166II GTNNAC 7 cut(s) 392, 439, 609, 693, 742, 772, 880
Hpy188I TCNGA 6 cut(s) 119, 302, 365, 637, 648, 856
Hpy188III TCNNGA 6 cut(s) 131, 197, 436, 446, 488, 887
Hpy8I GTNNAC 7 cut(s) 392, 439, 609, 693, 742, 772, 880
Hpy99I CGWCG 4 cut(s) 99, 452, 593, 655
HpyAV CCTTC 2 cut(s) 319, 442
HpyCH4III ACNGT 3 cut(s) 352, 458, 498
HpyCH4IV ACGT 2 cut(s) 106, 388
HpyCH4V TGCA 6 cut(s) 82, 218, 281, 329, 407, 713
HpyF10VI GCNNNNNNNGC 2 cut(s) 224, 719
HpyF3I CTNAG 3 cut(s) 473, 597, 645
HpySE526I ACGT 2 cut(s) 106, 388
Hsp92II CATG 2 cut(s) 40, 62
Ksp22I TGATCA 1 cut(s) 381
KspAI GTTAAC 1 cut(s) 772
Kzo9I GATC 5 cut(s) 127, 133, 381, 442, 529
LmnI GCTCC 2 cut(s) 49, 752
Lsp1109I GCAGC 3 cut(s) 53, 293, 767
LweI GCATC 1 cut(s) 268
MaeI CTAG 1 cut(s) 257
MaeII ACGT 2 cut(s) 106, 388
MaeIII GTNAC 2 cut(s) 517, 682
MalI GATC 5 cut(s) 129, 135, 383, 444, 531
MbiI CCGCTC 2 cut(s) 368, 750
MboI GATC 5 cut(s) 127, 133, 381, 442, 529
MboII GAAGA 1 cut(s) 251
MfeI CAATTG 2 cut(s) 402, 537
MhlI GDGCHC 2 cut(s) 264, 468
MluCI AATT 6 cut(s) 22, 158, 402, 537, 542, 795
MlyI GAGTC 1 cut(s) 238
MmeI TCCRAC 1 cut(s) 51
MnlI CCTC 3 cut(s) 403, 411, 779
MroXI GAANNNNTTC 1 cut(s) 795
MseI TTAA 3 cut(s) 144, 513, 771
MslI CAYNNNNRTG 2 cut(s) 63, 276
MspA1I CMGCKG 2 cut(s) 284, 758
MspI CCGG 1 cut(s) 806
MspR9I CCNGG 2 cut(s) 396, 806
MunI CAATTG 2 cut(s) 402, 537
MvaI CCWGG 1 cut(s) 396
MwoI GCNNNNNNNGC 2 cut(s) 224, 719
NciI CCSGG 1 cut(s) 806
NdeII GATC 5 cut(s) 127, 133, 381, 442, 529
NlaIII CATG 2 cut(s) 40, 62
NlaIV GGNNCC 3 cut(s) 235, 479, 634
NmuCI GTSAC 2 cut(s) 517, 682
PaqCI CACCTGC 2 cut(s) 362, 848
PcsI WCGNNNNNNNCGW 1 cut(s) 103
PdmI GAANNNNTTC 1 cut(s) 795
PfeI GAWTC 2 cut(s) 851, 866
PkrI GCNGC 4 cut(s) 43, 283, 370, 757
PleI GAGTC 1 cut(s) 238
PmaCI CACGTG 1 cut(s) 389
PmlI CACGTG 1 cut(s) 389
PpsI GAGTC 1 cut(s) 238
Ppu21I YACGTR 2 cut(s) 107, 389
Psp124BI GAGCTC 1 cut(s) 468
Psp6I CCWGG 1 cut(s) 394
PspCI CACGTG 1 cut(s) 389
PspGI CCWGG 1 cut(s) 394
PspN4I GGNNCC 3 cut(s) 235, 479, 634
PspPI GGNCC 4 cut(s) 233, 398, 420, 477
PstI CTGCAG 1 cut(s) 220
PvuII CAGCTG 1 cut(s) 758
RseI CAYNNNNRTG 2 cut(s) 63, 276
SacI GAGCTC 1 cut(s) 468
SaqAI TTAA 3 cut(s) 144, 513, 771
SatI GCNGC 4 cut(s) 42, 282, 369, 756
Sau3AI GATC 5 cut(s) 127, 133, 381, 442, 529
Sau96I GGNCC 4 cut(s) 233, 398, 420, 477
SchI GAGTC 1 cut(s) 238
ScrFI CCNGG 2 cut(s) 396, 806
SduI GDGCHC 2 cut(s) 264, 468
SetI ASST 7 cut(s) 46, 109, 376, 391, 468, 760, 842
SfaNI GCATC 1 cut(s) 268
SfcI CTRYAG 3 cut(s) 216, 454, 612
SinI GGWCC 2 cut(s) 233, 420
SmiMI CAYNNNNRTG 2 cut(s) 63, 276
Sse9I AATT 6 cut(s) 22, 158, 402, 537, 542, 795
SsiI CCGC 3 cut(s) 284, 368, 750
SspMI CTAG 1 cut(s) 257
SstI GAGCTC 1 cut(s) 468
StyD4I CCNGG 2 cut(s) 394, 804
TaaI ACNGT 3 cut(s) 352, 458, 498
TaiI ACGT 2 cut(s) 109, 391
TaqI TCGA 3 cut(s) 136, 660, 799
TaqII GACCGA 1 cut(s) 45
TasI AATT 6 cut(s) 22, 158, 402, 537, 542, 795
TauI GCSGC 1 cut(s) 371
TfiI GAWTC 2 cut(s) 851, 866
Tru1I TTAA 3 cut(s) 144, 513, 771
Tru9I TTAA 3 cut(s) 144, 513, 771
TscAI CASTG 2 cut(s) 501, 736
TseFI GTSAC 2 cut(s) 517, 682
TseI GCWGC 3 cut(s) 41, 281, 755
Tsp45I GTSAC 2 cut(s) 517, 682
TspDTI ATGAA 3 cut(s) 17, 185, 581
TspRI CASTG 2 cut(s) 501, 736
VpaK11BI GGWCC 2 cut(s) 233, 420
XapI RAATTY 2 cut(s) 158, 542
XcmI CCANNNNNNNNNTGG 1 cut(s) 341
XmiI GTMKAC 3 cut(s) 608, 692, 741
XmnI GAANNNNTTC 1 cut(s) 795
XspI CTAG 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.