RchiOBHm_Chr5g0033081

lactoylglutathione

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002543
Physical Location & Seq
Reverse (-)
26880945 .. 26883299
2355 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31220

Sequence Viewer

Length: 891 bp
ATGGCAGCTCCAAATAATCCCCATGACGATGTGTTGGAATGGGTGCAAAAAGATAATCGTCGTTTCCTACGTGCCAATATCCGAGTTGGTGATCTTGATCGAACTATTAAGTTTTACACAGAATTTTTTGGGATGAAAGTGTTGAGCAGGCAAGATTTCCCAGAACAAAAATACTCAACTGCAGTCGTTGCGTATGGACCCGAAGAGACTCATTTTGTTCTAGGGCTCACACACCACCACGATGCAGCGGACAAGTTAGACATCGGAACAGCCTTCAATCATTTTGGACTTGCAACCCAAGATGTTTATGGAACAGTTGAAAAAATCCGAGCGGCAGGTGGAGTGATCACACGTGAACCAGGGCCAATTGCAGAGGGTGGGAGGACCATCTTTGCCTTCGTGAACGATCCCGACGGCTACAGTTTTGAGCTCATCCTCAGGCCCCCAACTCCAGAACCACTGTGTCAAATATGTCTTAATGTGACTGATATTGATCGTTCAATTGAATTCTACCAAAAGGCATTGGGAATGAACCTACTATTGCCAAAGTACGACGCTTCTCAGTTCCAGTATACTATAGCGATGGTGGGTTATGGTTCCGATTTTACTCAGACGACGATTATCGAGTTGAAATATAACTATAATGTGACAGAGTATACGAAAGGAAATGGATATGCAATGCTTGCTATCGGCACTGATGATGTATACAAGAGCGGAGCAGCTGTTGAACTGGTTAACAAGGAACATGGAGGGGAAATAATTCGACCCCCGGCTGTAAATCCCAAAACCAACACCAAAATCACCTGCTTCCTTGATTCTGATGGGTTTGAATCGGTATTGGTAGACAATCAAGACTATGTTGGCTTTCTTGGAAAATCAAAGGATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00620 map00620
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

296

Amino Acids

33.05

Weight (kDa)

5.0

Isoelectric Point (pI)

27.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 23 - 144 2.8e-20 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase_4 PF13669 27 - 128 2.1e-07 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase PF00903 156 - 277 5e-10 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 326, 812
Acc36I ACCTGC 2 cut(s) 326, 812
AccBSI CCGCTC 2 cut(s) 332, 714
AccI GTMKAC 4 cut(s) 572, 656, 705, 843
AciI CCGC 3 cut(s) 248, 332, 714
AclWI GGATC 1 cut(s) 401
AcsI RAATTY 2 cut(s) 122, 506
AcvI CACGTG 1 cut(s) 353
AfaI GTAC 1 cut(s) 551
AflIII ACRYGT 1 cut(s) 350
AgsI TTSAA 7 cut(s) 277, 320, 501, 506, 631, 728, 830
AjnI CCWGG 1 cut(s) 358
AluBI AGCT 3 cut(s) 8, 430, 722
AluI AGCT 3 cut(s) 8, 430, 722
Alw21I GWGCWC 1 cut(s) 432
Alw26I GTCTC 1 cut(s) 200
AlwI GGATC 1 cut(s) 401
AoxI GGCC 2 cut(s) 362, 440
ApeKI GCWGC 3 cut(s) 5, 245, 719
ApoI RAATTY 2 cut(s) 122, 506
Asp700I GAANNNNTTC 1 cut(s) 759
AspS9I GGNCC 4 cut(s) 197, 362, 384, 441
AsuC2I CCSGG 1 cut(s) 770
AsuHPI GGTGA 2 cut(s) 101, 793
AvaII GGWCC 2 cut(s) 197, 384
AxyI CCTNAGG 1 cut(s) 437
BanII GRGCYC 2 cut(s) 228, 432
BbrPI CACGTG 1 cut(s) 353
Bbv12I GWGCWC 1 cut(s) 432
BbvI GCAGC 3 cut(s) 17, 257, 731
BccI CCATC 3 cut(s) 395, 577, 815
BceAI ACGGC 1 cut(s) 430
BciT130I CCWGG 1 cut(s) 360
BclI TGATCA 1 cut(s) 345
BcnI CCSGG 1 cut(s) 770
BcoDI GTCTC 1 cut(s) 200
BfaI CTAG 1 cut(s) 221
BfmI CTRYAG 3 cut(s) 180, 418, 576
BfuAI ACCTGC 2 cut(s) 326, 812
BisI GCNGC 4 cut(s) 6, 246, 333, 720
BlsI GCNGC 4 cut(s) 7, 247, 334, 721
Bme1390I CCNGG 2 cut(s) 360, 770
Bme18I GGWCC 2 cut(s) 197, 384
BmgT120I GGNCC 4 cut(s) 197, 362, 384, 441
BmiI GGNNCC 3 cut(s) 199, 443, 598
BmrFI CCNGG 2 cut(s) 360, 770
BmsI GCATC 1 cut(s) 232
BpmI CTGGAG 1 cut(s) 435
BpuMI CCSGG 1 cut(s) 770
BsaAI YACGTR 2 cut(s) 71, 353
BsaBI GATNNNNATC 2 cut(s) 96, 492
BsaJI CCNNGG 2 cut(s) 359, 768
BsaXI ACNNNNNCTCC 2 cut(s) 708, 738
Bse1I ACTGG 2 cut(s) 568, 735
Bse21I CCTNAGG 1 cut(s) 437
Bse3DI GCAATG 1 cut(s) 684
Bse8I GATNNNNATC 2 cut(s) 96, 492
BseBI CCWGG 1 cut(s) 360
BseDI CCNNGG 2 cut(s) 359, 768
BseGI GGATG 3 cut(s) 138, 432, 889
BseJI GATNNNNATC 2 cut(s) 96, 492
BseMI GCAATG 1 cut(s) 684
BseMII CTCAG 3 cut(s) 451, 575, 623
BseNI ACTGG 2 cut(s) 568, 735
BseXI GCAGC 3 cut(s) 17, 257, 731
BshFI GGCC 2 cut(s) 364, 442
BsiHKAI GWGCWC 1 cut(s) 432
BsiSI CCGG 1 cut(s) 770
BsmAI GTCTC 1 cut(s) 200
BsnI GGCC 2 cut(s) 364, 442
Bsp1286I GDGCHC 2 cut(s) 228, 432
Bsp143I GATC 5 cut(s) 91, 97, 345, 406, 493
BspACI CCGC 3 cut(s) 248, 332, 714
BspANI GGCC 2 cut(s) 364, 442
BspCNI CTCAG 3 cut(s) 450, 574, 622
BspLI GGNNCC 3 cut(s) 199, 443, 598
BspMAI CTGCAG 1 cut(s) 184
BspMI ACCTGC 2 cut(s) 326, 812
BspPI GGATC 1 cut(s) 401
BsrBI CCGCTC 2 cut(s) 332, 714
BsrDI GCAATG 1 cut(s) 684
BsrI ACTGG 2 cut(s) 568, 735
BssECI CCNNGG 2 cut(s) 359, 768
BssMI GATC 5 cut(s) 91, 97, 345, 406, 493
BssNAI GTATAC 3 cut(s) 573, 657, 706
Bst1107I GTATAC 3 cut(s) 573, 657, 706
Bst2UI CCWGG 1 cut(s) 360
Bst4CI ACNGT 3 cut(s) 316, 422, 462
Bst6I CTCTTC 1 cut(s) 198
BstAPI GCANNNNNTGC 2 cut(s) 188, 683
BstBAI YACGTR 2 cut(s) 71, 353
BstC8I GCNNGC 2 cut(s) 149, 684
BstDEI CTNAG 3 cut(s) 437, 561, 609
BstF5I GGATG 3 cut(s) 138, 432, 889
BstKTI GATC 5 cut(s) 94, 100, 348, 409, 496
BstMAI GTCTC 1 cut(s) 200
BstMBI GATC 5 cut(s) 91, 97, 345, 406, 493
BstMWI GCNNNNNNNGC 2 cut(s) 188, 683
BstNI CCWGG 1 cut(s) 360
BstSCI CCNGG 2 cut(s) 358, 768
BstSFI CTRYAG 3 cut(s) 180, 418, 576
BstV1I GCAGC 3 cut(s) 17, 257, 731
BstZ17I GTATAC 3 cut(s) 573, 657, 706
Bsu36I CCTNAGG 1 cut(s) 437
BsuRI GGCC 2 cut(s) 364, 442
BtgZI GCGATG 1 cut(s) 596
BtsCI GGATG 3 cut(s) 138, 432, 889
BtsIMutI CAGTG 2 cut(s) 458, 693
BveI ACCTGC 2 cut(s) 326, 812
Cac8I GCNNGC 2 cut(s) 149, 684
Cfr13I GGNCC 4 cut(s) 197, 362, 384, 441
CseI GACGC 1 cut(s) 563
Csp6I GTAC 1 cut(s) 550
CviAII CATG 2 cut(s) 23, 746
CviQI GTAC 1 cut(s) 550
DdeI CTNAG 3 cut(s) 437, 561, 609
DpnI GATC 5 cut(s) 93, 99, 347, 408, 495
DpnII GATC 5 cut(s) 91, 97, 345, 406, 493
Eam1104I CTCTTC 1 cut(s) 198
EarI CTCTTC 1 cut(s) 198
Ecl136II GAGCTC 1 cut(s) 430
Eco24I GRGCYC 2 cut(s) 228, 432
Eco47I GGWCC 2 cut(s) 197, 384
Eco53kI GAGCTC 1 cut(s) 430
Eco72I CACGTG 1 cut(s) 353
Eco81I CCTNAGG 1 cut(s) 437
EcoICRI GAGCTC 1 cut(s) 430
EcoO109I RGGNCCY 1 cut(s) 441
EcoRI GAATTC 1 cut(s) 506
EcoRII CCWGG 1 cut(s) 358
EcoT38I GRGCYC 2 cut(s) 228, 432
FaeI CATG 2 cut(s) 26, 749
FatI CATG 2 cut(s) 22, 745
FbaI TGATCA 1 cut(s) 345
FblI GTMKAC 4 cut(s) 572, 656, 705, 843
Fnu4HI GCNGC 4 cut(s) 6, 246, 333, 720
FokI GGATG 2 cut(s) 145, 419
FriOI GRGCYC 2 cut(s) 228, 432
Fsp4HI GCNGC 4 cut(s) 6, 246, 333, 720
FspBI CTAG 1 cut(s) 221
GluI GCNGC 4 cut(s) 6, 246, 333, 720
GsuI CTGGAG 1 cut(s) 435
HaeIII GGCC 2 cut(s) 364, 442
HapII CCGG 1 cut(s) 770
HgaI GACGC 1 cut(s) 563
Hin1II CATG 2 cut(s) 26, 749
HincII GTYRAC 1 cut(s) 736
HindII GTYRAC 1 cut(s) 736
HinfI GANTC 3 cut(s) 208, 815, 830
HpaI GTTAAC 1 cut(s) 736
HpaII CCGG 1 cut(s) 770
HphI GGTGA 2 cut(s) 101, 793
Hpy166II GTNNAC 7 cut(s) 356, 403, 573, 657, 706, 736, 844
Hpy188I TCNGA 6 cut(s) 83, 266, 329, 601, 612, 820
Hpy188III TCNNGA 5 cut(s) 95, 400, 410, 452, 851
Hpy8I GTNNAC 7 cut(s) 356, 403, 573, 657, 706, 736, 844
Hpy99I CGWCG 4 cut(s) 63, 416, 557, 619
HpyAV CCTTC 2 cut(s) 283, 406
HpyCH4III ACNGT 3 cut(s) 316, 422, 462
HpyCH4IV ACGT 2 cut(s) 70, 352
HpyCH4V TGCA 6 cut(s) 46, 182, 245, 293, 371, 677
HpyF10VI GCNNNNNNNGC 2 cut(s) 188, 683
HpyF3I CTNAG 3 cut(s) 437, 561, 609
HpySE526I ACGT 2 cut(s) 70, 352
Hsp92II CATG 2 cut(s) 26, 749
Ksp22I TGATCA 1 cut(s) 345
KspAI GTTAAC 1 cut(s) 736
Kzo9I GATC 5 cut(s) 91, 97, 345, 406, 493
LmnI GCTCC 2 cut(s) 13, 716
Lsp1109I GCAGC 3 cut(s) 17, 257, 731
LweI GCATC 1 cut(s) 232
MaeI CTAG 1 cut(s) 221
MaeII ACGT 2 cut(s) 70, 352
MaeIII GTNAC 2 cut(s) 481, 646
MalI GATC 5 cut(s) 93, 99, 347, 408, 495
MbiI CCGCTC 2 cut(s) 332, 714
MboI GATC 5 cut(s) 91, 97, 345, 406, 493
MboII GAAGA 1 cut(s) 215
MfeI CAATTG 2 cut(s) 366, 501
MhlI GDGCHC 2 cut(s) 228, 432
MluCI AATT 5 cut(s) 122, 366, 501, 506, 759
MlyI GAGTC 1 cut(s) 202
MmeI TCCRAC 1 cut(s) 15
MnlI CCTC 4 cut(s) 367, 375, 446, 743
MroXI GAANNNNTTC 1 cut(s) 759
MseI TTAA 3 cut(s) 108, 477, 735
MslI CAYNNNNRTG 2 cut(s) 27, 240
MspA1I CMGCKG 2 cut(s) 248, 722
MspI CCGG 1 cut(s) 770
MspR9I CCNGG 2 cut(s) 360, 770
MunI CAATTG 2 cut(s) 366, 501
MvaI CCWGG 1 cut(s) 360
MwoI GCNNNNNNNGC 2 cut(s) 188, 683
NciI CCSGG 1 cut(s) 770
NdeII GATC 5 cut(s) 91, 97, 345, 406, 493
NlaIII CATG 2 cut(s) 26, 749
NlaIV GGNNCC 3 cut(s) 199, 443, 598
NmuCI GTSAC 2 cut(s) 481, 646
PaqCI CACCTGC 2 cut(s) 326, 812
PcsI WCGNNNNNNNCGW 1 cut(s) 67
PdmI GAANNNNTTC 1 cut(s) 759
PfeI GAWTC 2 cut(s) 815, 830
PkrI GCNGC 4 cut(s) 7, 247, 334, 721
PleI GAGTC 1 cut(s) 202
PmaCI CACGTG 1 cut(s) 353
PmlI CACGTG 1 cut(s) 353
PpsI GAGTC 1 cut(s) 202
Ppu21I YACGTR 2 cut(s) 71, 353
Psp124BI GAGCTC 1 cut(s) 432
Psp6I CCWGG 1 cut(s) 358
PspCI CACGTG 1 cut(s) 353
PspGI CCWGG 1 cut(s) 358
PspN4I GGNNCC 3 cut(s) 199, 443, 598
PspPI GGNCC 4 cut(s) 197, 362, 384, 441
PstI CTGCAG 1 cut(s) 184
PvuII CAGCTG 1 cut(s) 722
RsaI GTAC 1 cut(s) 551
RsaNI GTAC 1 cut(s) 550
RseI CAYNNNNRTG 2 cut(s) 27, 240
SacI GAGCTC 1 cut(s) 432
SaqAI TTAA 3 cut(s) 108, 477, 735
SatI GCNGC 4 cut(s) 6, 246, 333, 720
Sau3AI GATC 5 cut(s) 91, 97, 345, 406, 493
Sau96I GGNCC 4 cut(s) 197, 362, 384, 441
SchI GAGTC 1 cut(s) 202
ScrFI CCNGG 2 cut(s) 360, 770
SduI GDGCHC 2 cut(s) 228, 432
SetI ASST 8 cut(s) 10, 73, 340, 355, 432, 537, 724, 806
SfaNI GCATC 1 cut(s) 232
SfcI CTRYAG 3 cut(s) 180, 418, 576
SinI GGWCC 2 cut(s) 197, 384
SmiMI CAYNNNNRTG 2 cut(s) 27, 240
Sse9I AATT 5 cut(s) 122, 366, 501, 506, 759
SsiI CCGC 3 cut(s) 248, 332, 714
SspMI CTAG 1 cut(s) 221
SstI GAGCTC 1 cut(s) 432
StyD4I CCNGG 2 cut(s) 358, 768
TaaI ACNGT 3 cut(s) 316, 422, 462
TaiI ACGT 2 cut(s) 73, 355
TaqI TCGA 3 cut(s) 100, 624, 763
TasI AATT 5 cut(s) 122, 366, 501, 506, 759
TauI GCSGC 1 cut(s) 335
TfiI GAWTC 2 cut(s) 815, 830
Tru1I TTAA 3 cut(s) 108, 477, 735
Tru9I TTAA 3 cut(s) 108, 477, 735
TscAI CASTG 2 cut(s) 465, 700
TseFI GTSAC 2 cut(s) 481, 646
TseI GCWGC 3 cut(s) 5, 245, 719
Tsp45I GTSAC 2 cut(s) 481, 646
TspDTI ATGAA 2 cut(s) 149, 545
TspRI CASTG 2 cut(s) 465, 700
VpaK11BI GGWCC 2 cut(s) 197, 384
XapI RAATTY 2 cut(s) 122, 506
XcmI CCANNNNNNNNNTGG 1 cut(s) 305
XmiI GTMKAC 4 cut(s) 572, 656, 705, 843
XmnI GAANNNNTTC 1 cut(s) 759
XspI CTAG 1 cut(s) 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.