Rorug05G0136500

lactoylglutathione

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0002543
Physical Location & Seq
Forward (+)
12308699 .. 12308974
276 bp
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UTR
Exon/CDS
Intron
Rorug05G0136500.1

Sequence Viewer

Length: 276 bp
ATGAGGTACTTCCACAAGCTCCGCAACAAGTTCTACTGCCCCATCGTCAACGTCAACGTCGACAAGCTCTGGTCACTGGTGCCGCAGGAGGTCAAGGACAAGGCCACCAAAAACGACGTGCCGTTGATTGACGTGACGCAGCACGGGTTCTTCAAGATTTTGGGCAAGGGGAAGCTGCCGGCGGACCAGCCCGTGGTGGTGAAGGCCAAGCTCATTTCGAAGGTCGCCGAGAAGAAGATCAAGGAGGCCGGCGGCGCTGTGGTTCTCACCGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00620 map00620
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

91

Amino Acids

10.17

Weight (kDa)

9.86

Isoelectric Point (pI)

23.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L27A PF00828 18 - 88 1.8e-16 Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 79
AccB7I CCANNNNNTGG 1 cut(s) 193
AccI GTMKAC 1 cut(s) 60
AciI CCGC 5 cut(s) 22, 83, 182, 252, 270
AfaI GTAC 1 cut(s) 8
AfiI CCNNNNNNNGG 1 cut(s) 193
AgsI TTSAA 1 cut(s) 154
AjiI CACGTC 2 cut(s) 118, 133
AluBI AGCT 4 cut(s) 19, 67, 175, 211
AluI AGCT 4 cut(s) 19, 67, 175, 211
AoxI GGCC 3 cut(s) 102, 204, 246
ApeKI GCWGC 2 cut(s) 139, 175
AspLEI GCGC 1 cut(s) 257
AspS9I GGNCC 1 cut(s) 184
AsuHPI GGTGA 2 cut(s) 211, 259
AsuII TTCGAA 1 cut(s) 218
AvaII GGWCC 1 cut(s) 184
BanI GGYRCC 1 cut(s) 79
BbvI GCAGC 2 cut(s) 151, 162
BccI CCATC 1 cut(s) 50
BceAI ACGGC 1 cut(s) 106
BfaI CTAG 1 cut(s) 274
BfoI RGCGCY 1 cut(s) 258
BisI GCNGC 4 cut(s) 83, 140, 176, 253
BlsI GCNGC 4 cut(s) 84, 141, 177, 254
Bme18I GGWCC 1 cut(s) 184
BmgBI CACGTC 2 cut(s) 118, 133
BmgT120I GGNCC 1 cut(s) 184
BmiI GGNNCC 1 cut(s) 81
Bpu14I TTCGAA 1 cut(s) 218
BsaJI CCNNGG 1 cut(s) 192
Bsc4I CCNNNNNNNGG 1 cut(s) 193
Bse118I RCCGGY 2 cut(s) 178, 248
Bse1I ACTGG 1 cut(s) 81
BseDI CCNNGG 1 cut(s) 192
BseLI CCNNNNNNNGG 1 cut(s) 193
BseNI ACTGG 1 cut(s) 81
BseXI GCAGC 2 cut(s) 151, 162
BshFI GGCC 3 cut(s) 104, 206, 248
BshNI GGYRCC 1 cut(s) 79
BsiSI CCGG 2 cut(s) 179, 249
BslI CCNNNNNNNGG 1 cut(s) 193
BsnI GGCC 3 cut(s) 104, 206, 248
Bsp119I TTCGAA 1 cut(s) 218
Bsp143I GATC 1 cut(s) 237
BspACI CCGC 5 cut(s) 22, 83, 182, 252, 270
BspANI GGCC 3 cut(s) 104, 206, 248
BspLI GGNNCC 1 cut(s) 81
BspT104I TTCGAA 1 cut(s) 218
BspT107I GGYRCC 1 cut(s) 79
BsrFI RCCGGY 2 cut(s) 178, 248
BsrI ACTGG 1 cut(s) 81
BssAI RCCGGY 2 cut(s) 178, 248
BssECI CCNNGG 1 cut(s) 192
BssMI GATC 1 cut(s) 237
BstBI TTCGAA 1 cut(s) 218
BstC8I GCNNGC 2 cut(s) 180, 250
BstDSI CCRYGG 1 cut(s) 192
BstH2I RGCGCY 1 cut(s) 258
BstHHI GCGC 1 cut(s) 257
BstKTI GATC 1 cut(s) 240
BstMBI GATC 1 cut(s) 237
BstMWI GCNNNNNNNGC 1 cut(s) 254
BstV1I GCAGC 2 cut(s) 151, 162
BsuRI GGCC 3 cut(s) 104, 206, 248
BtgI CCRYGG 1 cut(s) 192
BtrI CACGTC 2 cut(s) 118, 133
BtsIMutI CAGTG 1 cut(s) 74
Cac8I GCNNGC 2 cut(s) 180, 250
CfoI GCGC 1 cut(s) 257
Cfr10I RCCGGY 2 cut(s) 178, 248
Cfr13I GGNCC 1 cut(s) 184
CseI GACGC 1 cut(s) 145
Csp6I GTAC 1 cut(s) 7
CviJI RGCY 8 cut(s) 19, 67, 104, 175, 190, 206, 211, 248
CviKI_1 RGCY 8 cut(s) 19, 67, 104, 175, 190, 206, 211, 248
CviQI GTAC 1 cut(s) 7
DpnI GATC 1 cut(s) 239
DpnII GATC 1 cut(s) 237
EciI GGCGGA 1 cut(s) 197
Eco47I GGWCC 1 cut(s) 184
FblI GTMKAC 1 cut(s) 60
Fnu4HI GCNGC 4 cut(s) 83, 140, 176, 253
Fsp4HI GCNGC 4 cut(s) 83, 140, 176, 253
FspBI CTAG 1 cut(s) 274
GlaI GCGC 1 cut(s) 256
GluI GCNGC 4 cut(s) 83, 140, 176, 253
HaeII RGCGCY 1 cut(s) 258
HaeIII GGCC 3 cut(s) 104, 206, 248
HapII CCGG 2 cut(s) 179, 249
HgaI GACGC 1 cut(s) 145
HhaI GCGC 1 cut(s) 257
Hin6I GCGC 1 cut(s) 255
HinP1I GCGC 1 cut(s) 255
HincII GTYRAC 3 cut(s) 49, 55, 61
HindII GTYRAC 3 cut(s) 49, 55, 61
HpaII CCGG 2 cut(s) 179, 249
HphI GGTGA 2 cut(s) 211, 259
Hpy166II GTNNAC 3 cut(s) 49, 55, 61
Hpy188III TCNNGA 1 cut(s) 154
Hpy8I GTNNAC 3 cut(s) 49, 55, 61
Hpy99I CGWCG 2 cut(s) 62, 119
HpyAV CCTTC 2 cut(s) 196, 214
HpyCH4IV ACGT 4 cut(s) 51, 57, 117, 132
HpyF10VI GCNNNNNNNGC 1 cut(s) 254
HpySE526I ACGT 4 cut(s) 51, 57, 117, 132
HspAI GCGC 1 cut(s) 255
KroI GCCGGC 2 cut(s) 178, 248
KroNI GCCGGC 2 cut(s) 180, 250
Kzo9I GATC 1 cut(s) 237
LmnI GCTCC 1 cut(s) 24
LpnPI CCDG 6 cut(s) 55, 62, 71, 192, 200, 262
Lsp1109I GCAGC 2 cut(s) 151, 162
MaeI CTAG 1 cut(s) 274
MaeII ACGT 4 cut(s) 51, 57, 117, 132
MaeIII GTNAC 2 cut(s) 72, 133
MalI GATC 1 cut(s) 239
MboI GATC 1 cut(s) 237
MboII GAAGA 3 cut(s) 142, 244, 247
MnlI CCTC 2 cut(s) 82, 238
MroNI GCCGGC 2 cut(s) 178, 248
MspI CCGG 2 cut(s) 179, 249
MwoI GCNNNNNNNGC 1 cut(s) 254
NaeI GCCGGC 2 cut(s) 180, 250
NdeII GATC 1 cut(s) 237
NgoMIV GCCGGC 2 cut(s) 178, 248
NlaIV GGNNCC 1 cut(s) 81
NmeAIII GCCGAG 1 cut(s) 253
NmuCI GTSAC 2 cut(s) 72, 133
NspV TTCGAA 1 cut(s) 218
PcsI WCGNNNNNNNCGW 1 cut(s) 57
PdiI GCCGGC 2 cut(s) 180, 250
PflMI CCANNNNNTGG 1 cut(s) 193
PkrI GCNGC 4 cut(s) 84, 141, 177, 254
PspN4I GGNNCC 1 cut(s) 81
PspPI GGNCC 1 cut(s) 184
RsaI GTAC 1 cut(s) 8
RsaNI GTAC 1 cut(s) 7
SalI GTCGAC 1 cut(s) 59
SatI GCNGC 4 cut(s) 83, 140, 176, 253
Sau3AI GATC 1 cut(s) 237
Sau96I GGNCC 1 cut(s) 184
SfuI TTCGAA 1 cut(s) 218
SinI GGWCC 1 cut(s) 184
SsiI CCGC 5 cut(s) 22, 83, 182, 252, 270
SspMI CTAG 1 cut(s) 274
TaiI ACGT 4 cut(s) 54, 60, 120, 135
TaqI TCGA 2 cut(s) 60, 218
TauI GCSGC 2 cut(s) 85, 255
TscAI CASTG 1 cut(s) 81
TseFI GTSAC 2 cut(s) 72, 133
TseI GCWGC 2 cut(s) 139, 175
Tsp45I GTSAC 2 cut(s) 72, 133
TspRI CASTG 1 cut(s) 81
Van91I CCANNNNNTGG 1 cut(s) 193
VpaK11BI GGWCC 1 cut(s) 184
XmiI GTMKAC 1 cut(s) 60
XspI CTAG 1 cut(s) 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.