Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0001738

Physical Location & Seq

Genomic Coordinates Reverse (-)

35596850 .. 35598781

Gene Structure 1932 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1272600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1932 bp

ATGCCCATCCAAACAGCTCCTTATGAAACACTAATGTCCTCCTCCTTCCTCTTCCTCCTCCTCCTCCTCCTCCTCCTCTCCTCCTCCCCTACCCCATCTCTCTCCCAGCTAAACTCCACCGCTCCATGCCCCCTCAACTTCACCATCCTCCGCCAGTTCAACCCGGGCTCCCATAACCGCCGCCGCTACGACCTCAACACCGGGTGCCAGTACTTCGTCCAGGGCCTCCGCCTCGTCGACTCCGAGTACCTCCGCCGCACCGGCAACTTCCTTCCCCCACCCAACGCCTCCGAGTCCTGCTGGGCGGACTACCAAGCCCTAGCCTCCGAGCTCGTCCCCAATTTCGACATTCGGGCCAACTGCGGCTTCCAGACCAGCTGGATCTCCGAAGGATGCACGAACGTCTCCACCAGGTCCCAATTCGAATCCCAAGTCGGAAACACCTCGCTCAACGACGTCGCCTCCGCCTGCAACCAGTCTCTCGGAAATGGATCACCCTGCGCCCTCTGCACCACCCGAATGACCTCGCAGAGCTCATTCTTGACCGGCGAGTCCGTCGGAAACATCTCCACCTGCACGGCGTACCCGTCCATTTACGTGGCGGCTTTCCTCGCACCGACCGATATGGGCACAGCCGAGTGCTTGTTCGCGCTCAGAACCCTGGCGGAGGGATCCGGTAAGAAGAAAAAGAAGGTAATTTTGATTGTATTGGTTGCTAGCGGTGTCGGATTGCTGATTGTGATCGCCGGCATTTGGTTTTTGTGGCATAAATATAAAGACTTCAGGGCTAAGAAGAGGAAAAAAGATATTAGTGATAAAATTGAACAGGGATTAGGTTCTGCTTTAGAGTCGATTAGTGGAAGTACTAATGTAATTAAGTTTAAATACGAAGAGATTAGTGCTGCAACCAAGAATTTCTCCAGGGACAATGTAATTGGGAGAGGAGGATATGGAAATGTGTACAAGGGAATTCTGGATGATGGTTCTGAAGTTGCATTGAAGAGGTTCAAGAACTGCTCGGCTGCTGGCGACACAATTTTCGCGCACGAGGTTGAGGTGATTGCGAGTGTTAGGCACGTCAATCTCGTTGCATTGAGAGGTTATTGTATTGCCAATACTCCTTTGATGGGTCACCAGAGGATCATTGTGTGTGATTTGATGAAGAATGGAAGTCTCCACGACCATTTATTCGGGTCGTTTGAGATCAAGCTTAGTTGGCCAATTCGTCAGAGGATTGCGCTGGGGATGGCGAGGGGATTGGCTTATTTGCATTATGGGGCTCAACCAACTATTATCCATAGGGACATCAAAGCTAATAACATACTCTTGGATGAGACATTCGAGGCCAAGGTAGCAGATTTTGGGCTCGCGAAGTTTACACCTGAGGGAATGACGCATTTGAGCACGAGGGTGGCTGGAACAATGGGTTATGTTGCTCCAGAGTATGCGTTGTATGGACAGTTGACGGATAAAACTGATGTGTATAGTTTTGGTGTTGTGCTTCTTGAGCTTTTGAGTGGAAAGAAGGCGCTCCATGTGAGTAATGATAGTCAGCCTTCACTTGTAACTGATTGGGCGTGGGAGTTGGTGAGGAAAGGAAGACCGCTAGATGTTATTGAAAATGATATGCCGGAGAAGGGTGCACCCGAAGTTCTAGAGAAGTATGTATTGATTGCCGTTCTTTGTTCTCATCCGCAGTTGCATGCTAGGCCAACGATGGATCAGGCTGTGAAAATGTTGGAAACAGGTGTATCGGTTCCATCAATTCCAGAAAGACCAATTCCCATTGTGTCAGATATTGACGATATTGAAAGATACAGCAGCAGTGGTTCAATCCAGCTCTCTACTACATGTGGTTTTCAGACTTATAAATTCGAAGGTAACCTACCTTCTAGCCCTAATGGAGACGGGGAAAGCTCCCATAGCAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko03010 map03010

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

644

Amino Acids

70.38

Weight (kDa)

7.18

Isoelectric Point (pI)

40.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SPARK	PF19160	38 - 192	1.1e-34	SPARK
Pkinase	PF00069	307 - 578	1e-41	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	309 - 578	2.6e-39	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001738)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G48010
fragaria_vesca	FvH4_2g24291 FvH4_2g24291 FvH4_6g05000 FvH4_6g05020
malus_domestica	MD03G1272600.v1.1 MD04G1211500.v1.1 MD11G1293500.v1.1 MD12G1226500.v1.1
prunus_persica	Prupe.6G332100_v2.0.a1 Prupe.6G332200_v2.0.a1 Prupe.8G248800_v2.0.a1
pyrus_communis	pycom03g21710 pycom11g25970
rosa_chinensis	RchiOBHm_Chr3g0453511 RchiOBHm_Chr3g0453521 RchiOBHm_Chr5g0002211 RchiOBHm_Chr6g0292151
rosa_laevigata	RLG00000012057 RLG00000025506 RLG00000025507 RLG00000031008
rosa_multiflora	Rmu_co8460761.1_g000001 Rmu_sc0000510.1_g000020
rosa_roxburghii	Rroxscaffold_1G00073750 Rroxscaffold_2G00125960 Rroxscaffold_6G00430490 Rroxscaffold_7G00175400
rosa_rugosa	Rorug02G0649700 Rorug02G0649800 Rorug06G0223800
rosa_samantha	Rh1BG359400 Rh3AG052600 Rh3AG052700 Rh3BG054100 Rh3BG054200 Rh3CG053400 Rh3CG053500 Rh3DG054200 Rh3DG054300 Rh6AG337200 Rh6BG344400 Rh6CG351200 Rh6DG337400 Rh7BG191800
rosa_wichuraiana	Rw3G004050 Rw3G004060 Rw6G029350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1869
AarI	CACCTGC	1 cut(s)	581
AasI	GACNNNNNNGTC	1 cut(s)	550
AatII	GACGTC	1 cut(s)	459
Acc36I	ACCTGC	1 cut(s)	581
AccB1I	GGYRCC	1 cut(s)	204
AccBSI	CCGCTC	1 cut(s)	122
AccI	GTMKAC	1 cut(s)	237
AccII	CGCG	3 cut(s)	650, 1043, 1370
AclWI	GGATC	6 cut(s)	389, 499, 666, 679, 1148, 1728
AcoI	YGGCCR	1 cut(s)	1217
AcsI	RAATTY	3 cut(s)	913, 969, 1871
AcuI	CTGAAG	2 cut(s)	766, 1008
AcyI	GRCGYC	1 cut(s)	456
AdeI	CACNNNGTG	1 cut(s)	204
AfaI	GTAC	5 cut(s)	212, 248, 584, 865, 962
AfiI	CCNNNNNNNGG	4 cut(s)	667, 753, 1127, 1637
AflIII	ACRYGT	1 cut(s)	1850
AgsI	TTSAA	7 cut(s)	160, 824, 1000, 1009, 1619, 1811, 1833
AjiI	CACGTC	1 cut(s)	1078
AjnI	CCWGG	4 cut(s)	219, 410, 660, 920
AjuI	GAANNNNNNNTTGG	2 cut(s)	350, 382
AleI	CACNNNNGTG	1 cut(s)	1409
Alw21I	GWGCWC	4 cut(s)	333, 536, 1406, 1645
Alw26I	GTCTC	5 cut(s)	409, 483, 1178, 1328, 1899
Alw44I	GTGCAC	1 cut(s)	1641
AlwI	GGATC	6 cut(s)	389, 499, 666, 679, 1148, 1728
Ama87I	CYCGRG	1 cut(s)	163
AoxI	GGCC	5 cut(s)	223, 354, 1217, 1344, 1709
ApaLI	GTGCAC	1 cut(s)	1641
ApeKI	GCWGC	3 cut(s)	902, 1022, 1821
ApoI	RAATTY	3 cut(s)	913, 969, 1871
ArsI	GACNNNNNNTTYG	6 cut(s)	417, 449, 1022, 1054, 1172, 1204
Asp700I	GAANNNNTTC	1 cut(s)	1004
AspLEI	GCGC	5 cut(s)	503, 652, 1045, 1240, 1531
AspS9I	GGNCC	3 cut(s)	223, 354, 414
AsuC2I	CCSGG	3 cut(s)	164, 165, 202
AsuHPI	GGTGA	5 cut(s)	133, 486, 1069, 1124, 1600
AsuII	TTCGAA	2 cut(s)	423, 1875
AsuNHI	GCTAGC	1 cut(s)	716
AvaI	CYCGRG	1 cut(s)	163
AvaII	GGWCC	1 cut(s)	414
AxyI	CCTNAGG	1 cut(s)	1383
BaeGI	GKGCMC	2 cut(s)	632, 1645
BaeI	ACNNNNGTAYC	2 cut(s)	1734, 1767
BalI	TGGCCA	1 cut(s)	1219
BamHI	GGATCC	1 cut(s)	671
BanI	GGYRCC	1 cut(s)	204
BanII	GRGCYC	5 cut(s)	170, 333, 536, 1282, 1368
BarI	GAAGNNNNNNTAC	4 cut(s)	1869, 1873, 1901, 1905
BauI	CACGAG	2 cut(s)	1046, 1405
BbsI	GAAGAC	1 cut(s)	1606
Bbv12I	GWGCWC	4 cut(s)	333, 536, 1406, 1645
BbvI	GCAGC	3 cut(s)	889, 1009, 1833
BccI	CCATC	8 cut(s)	14, 103, 152, 974, 1120, 1240, 1711, 1768
BceAI	ACGGC	2 cut(s)	594, 1661
BciT130I	CCWGG	4 cut(s)	221, 412, 662, 922
BcnI	CCSGG	3 cut(s)	164, 165, 202
BcoDI	GTCTC	5 cut(s)	409, 483, 1178, 1328, 1899
BfaI	CTAG	6 cut(s)	320, 717, 1607, 1655, 1707, 1893
BfoI	RGCGCY	1 cut(s)	1532
BfuAI	ACCTGC	1 cut(s)	581
BglI	GCCNNNNNGGC	1 cut(s)	261
BisI	GCNGC	8 cut(s)	181, 184, 256, 364, 603, 903, 1023, 1822
BlsI	GCNGC	8 cut(s)	182, 185, 257, 365, 604, 904, 1024, 1823
BmcAI	AGTACT	2 cut(s)	212, 865
Bme1390I	CCNGG	7 cut(s)	164, 165, 202, 221, 412, 662, 922
Bme18I	GGWCC	1 cut(s)	414
BmeT110I	CYCGRG	1 cut(s)	163
BmgBI	CACGTC	1 cut(s)	1078
BmgT120I	GGNCC	3 cut(s)	223, 354, 414
BmiI	GGNNCC	5 cut(s)	169, 206, 416, 673, 1758
BmrFI	CCNGG	7 cut(s)	164, 165, 202, 221, 412, 662, 922
BmsI	GCATC	1 cut(s)	383
BmtI	GCTAGC	1 cut(s)	720
BpiI	GAAGAC	1 cut(s)	1606
BpmI	CTGGAG	2 cut(s)	904, 1422
Bpu14I	TTCGAA	2 cut(s)	423, 1875
BpuEI	CTTGAG	1 cut(s)	1526
BpuMI	CCSGG	3 cut(s)	164, 165, 202
BsaAI	YACGTR	1 cut(s)	598
BsaBI	GATNNNNATC	1 cut(s)	740
BsaHI	GRCGYC	1 cut(s)	456
BsaJI	CCNNGG	5 cut(s)	163, 220, 660, 921, 1347
BsaWI	WCCGGW	1 cut(s)	674
BsaXI	ACNNNNNCTCC	4 cut(s)	152, 182, 446, 476
Bsc4I	CCNNNNNNNGG	4 cut(s)	667, 753, 1127, 1637
Bse118I	RCCGGY	3 cut(s)	260, 545, 746
Bse1I	ACTGG	3 cut(s)	154, 208, 475
Bse21I	CCTNAGG	1 cut(s)	1383
Bse8I	GATNNNNATC	1 cut(s)	740
BseBI	CCWGG	4 cut(s)	221, 412, 662, 922
BseDI	CCNNGG	5 cut(s)	163, 220, 660, 921, 1347
BseGI	GGATG	7 cut(s)	6, 144, 398, 982, 1251, 1336, 1690
BseJI	GATNNNNATC	1 cut(s)	740
BseLI	CCNNNNNNNGG	4 cut(s)	667, 753, 1127, 1637
BseMII	CTCAG	2 cut(s)	667, 1374
BseNI	ACTGG	3 cut(s)	154, 208, 475
BseSI	GKGCMC	2 cut(s)	632, 1645
BseXI	GCAGC	3 cut(s)	889, 1009, 1833
BseYI	CCCAGC	3 cut(s)	105, 300, 1240
BsgI	GTGCAG	2 cut(s)	493, 559
Bsh1236I	CGCG	3 cut(s)	650, 1043, 1370
Bsh1285I	CGRYCG	1 cut(s)	621
BshFI	GGCC	5 cut(s)	225, 356, 1219, 1346, 1711
BshNI	GGYRCC	1 cut(s)	204
BsiEI	CGRYCG	1 cut(s)	621
BsiHKAI	GWGCWC	4 cut(s)	333, 536, 1406, 1645
BsiHKCI	CYCGRG	1 cut(s)	163
BsiSI	CCGG	7 cut(s)	164, 201, 261, 546, 675, 747, 1631
BslFI	GGGAC	4 cut(s)	320, 400, 938, 1316
BslI	CCNNNNNNNGG	4 cut(s)	667, 753, 1127, 1637
BsmAI	GTCTC	5 cut(s)	409, 483, 1178, 1328, 1899
BsmBI	CGTCTC	2 cut(s)	409, 1899
BsmFI	GGGAC	4 cut(s)	320, 400, 938, 1316
BsnI	GGCC	5 cut(s)	225, 356, 1219, 1346, 1711
BsoBI	CYCGRG	1 cut(s)	163
Bsp119I	TTCGAA	2 cut(s)	423, 1875
Bsp1286I	GDGCHC	8 cut(s)	170, 333, 536, 632, 1282, 1368, 1406, 1645
Bsp1407I	TGTACA	1 cut(s)	960
Bsp143I	GATC	7 cut(s)	381, 491, 671, 741, 1140, 1203, 1720
Bsp68I	TCGCGA	1 cut(s)	1370
BspANI	GGCC	5 cut(s)	225, 356, 1219, 1346, 1711
BspCNI	CTCAG	2 cut(s)	666, 1375
BspFNI	CGCG	3 cut(s)	650, 1043, 1370
BspLI	GGNNCC	5 cut(s)	169, 206, 416, 673, 1758
BspMI	ACCTGC	1 cut(s)	581
BspOI	GCTAGC	1 cut(s)	720
BspPI	GGATC	6 cut(s)	389, 499, 666, 679, 1148, 1728
BspT104I	TTCGAA	2 cut(s)	423, 1875
BspT107I	GGYRCC	1 cut(s)	204
BsrBI	CCGCTC	1 cut(s)	122
BsrFI	RCCGGY	3 cut(s)	260, 545, 746
BsrGI	TGTACA	1 cut(s)	960
BsrI	ACTGG	3 cut(s)	154, 208, 475
BssAI	RCCGGY	3 cut(s)	260, 545, 746
BssECI	CCNNGG	5 cut(s)	163, 220, 660, 921, 1347
BssMI	GATC	7 cut(s)	381, 491, 671, 741, 1140, 1203, 1720
BssNI	GRCGYC	1 cut(s)	456
BssSI	CACGAG	2 cut(s)	1046, 1405
BssT1I	CCWWGG	1 cut(s)	1347
Bst2BI	CACGAG	2 cut(s)	1046, 1405
Bst2UI	CCWGG	4 cut(s)	221, 412, 662, 922
Bst4CI	ACNGT	1 cut(s)	1461
Bst6I	CTCTTC	4 cut(s)	56, 788, 885, 995
BstACI	GRCGYC	1 cut(s)	456
BstAUI	TGTACA	1 cut(s)	960
BstBAI	YACGTR	1 cut(s)	598
BstBI	TTCGAA	2 cut(s)	423, 1875
BstC8I	GCNNGC	6 cut(s)	469, 718, 748, 1027, 1368, 1704
BstDEI	CTNAG	4 cut(s)	653, 789, 1211, 1383
BstEII	GGTNACC	2 cut(s)	1130, 1880
BstENI	CCTNNNNNAGG	1 cut(s)	665
BstF5I	GGATG	7 cut(s)	6, 144, 398, 982, 1251, 1336, 1690
BstFNI	CGCG	3 cut(s)	650, 1043, 1370
BstH2I	RGCGCY	1 cut(s)	1532
BstHHI	GCGC	5 cut(s)	503, 652, 1045, 1240, 1531
BstKTI	GATC	7 cut(s)	384, 494, 674, 744, 1143, 1206, 1723
BstMAI	GTCTC	5 cut(s)	409, 483, 1178, 1328, 1899
BstMBI	GATC	7 cut(s)	381, 491, 671, 741, 1140, 1203, 1720
BstMCI	CGRYCG	1 cut(s)	621
BstMWI	GCNNNNNNNGC	8 cut(s)	261, 507, 611, 1216, 1352, 1507, 1708, 1923
BstNI	CCWGG	4 cut(s)	221, 412, 662, 922
BstNSI	RCATGY	2 cut(s)	1706, 1854
BstPI	GGTNACC	2 cut(s)	1130, 1880
BstSCI	CCNGG	7 cut(s)	162, 163, 200, 219, 410, 660, 920
BstSLI	GKGCMC	2 cut(s)	632, 1645
BstUI	CGCG	3 cut(s)	650, 1043, 1370
BstV1I	GCAGC	3 cut(s)	889, 1009, 1833
BstV2I	GAAGAC	1 cut(s)	1606
BstX2I	RGATCY	2 cut(s)	381, 671
BstXI	CCANNNNNNTGG	1 cut(s)	598
BstYI	RGATCY	2 cut(s)	381, 671
Bsu36I	CCTNAGG	1 cut(s)	1383
BsuRI	GGCC	5 cut(s)	225, 356, 1219, 1346, 1711
BtrI	CACGTC	1 cut(s)	1078
BtsCI	GGATG	7 cut(s)	6, 144, 398, 982, 1251, 1336, 1690
BtsI	GCAGTG	1 cut(s)	1831
BtsIMutI	CAGTG	1 cut(s)	1831
BtuMI	TCGCGA	1 cut(s)	1370
BveI	ACCTGC	1 cut(s)	581
Cac8I	GCNNGC	6 cut(s)	469, 718, 748, 1027, 1368, 1704
CfoI	GCGC	5 cut(s)	503, 652, 1045, 1240, 1531
Cfr10I	RCCGGY	3 cut(s)	260, 545, 746
Cfr13I	GGNCC	3 cut(s)	223, 354, 414
Cfr9I	CCCGGG	1 cut(s)	163
CseI	GACGC	1 cut(s)	1402
CsiI	ACCWGGT	1 cut(s)	410
Csp6I	GTAC	5 cut(s)	211, 247, 583, 864, 961
CviAII	CATG	4 cut(s)	126, 1535, 1703, 1851
CviQI	GTAC	5 cut(s)	211, 247, 583, 864, 961
DdeI	CTNAG	4 cut(s)	653, 789, 1211, 1383
DpnI	GATC	7 cut(s)	383, 493, 673, 743, 1142, 1205, 1722
DpnII	GATC	7 cut(s)	381, 491, 671, 741, 1140, 1203, 1720
DraI	TTTAAA	1 cut(s)	883
DraIII	CACNNNGTG	1 cut(s)	204
DrdI	GACNNNNNNGTC	1 cut(s)	550
DseDI	GACNNNNNNGTC	1 cut(s)	550
EaeI	YGGCCR	1 cut(s)	1217
Eam1104I	CTCTTC	4 cut(s)	56, 788, 885, 995
EarI	CTCTTC	4 cut(s)	56, 788, 885, 995
EciI	GGCGGA	6 cut(s)	140, 218, 242, 320, 454, 680
Ecl136II	GAGCTC	2 cut(s)	331, 534
Eco130I	CCWWGG	1 cut(s)	1347
Eco24I	GRGCYC	5 cut(s)	170, 333, 536, 1282, 1368
Eco47I	GGWCC	1 cut(s)	414
Eco53kI	GAGCTC	2 cut(s)	331, 534
Eco57I	CTGAAG	2 cut(s)	766, 1008
Eco81I	CCTNAGG	1 cut(s)	1383
Eco88I	CYCGRG	1 cut(s)	163
Eco91I	GGTNACC	2 cut(s)	1130, 1880
EcoICRI	GAGCTC	2 cut(s)	331, 534
EcoNI	CCTNNNNNAGG	1 cut(s)	665
EcoO109I	RGGNCCY	2 cut(s)	223, 414
EcoO65I	GGTNACC	2 cut(s)	1130, 1880
EcoRI	GAATTC	1 cut(s)	969
EcoRII	CCWGG	4 cut(s)	219, 410, 660, 920
EcoT14I	CCWWGG	1 cut(s)	1347
EcoT38I	GRGCYC	5 cut(s)	170, 333, 536, 1282, 1368
ErhI	CCWWGG	1 cut(s)	1347
Esp3I	CGTCTC	2 cut(s)	409, 1899
FaeI	CATG	4 cut(s)	129, 1538, 1706, 1854
FaqI	GGGAC	4 cut(s)	320, 400, 938, 1316
FatI	CATG	4 cut(s)	125, 1534, 1702, 1850
FblI	GTMKAC	1 cut(s)	237
Fnu4HI	GCNGC	8 cut(s)	181, 184, 256, 364, 603, 903, 1023, 1822
FokI	GGATG	6 cut(s)	131, 405, 989, 1258, 1343, 1677
FriOI	GRGCYC	5 cut(s)	170, 333, 536, 1282, 1368
Fsp4HI	GCNGC	8 cut(s)	181, 184, 256, 364, 603, 903, 1023, 1822
FspBI	CTAG	6 cut(s)	320, 717, 1607, 1655, 1707, 1893
GlaI	GCGC	5 cut(s)	502, 651, 1044, 1239, 1530
GluI	GCNGC	8 cut(s)	181, 184, 256, 364, 603, 903, 1023, 1822
GsaI	CCCAGC	3 cut(s)	109, 304, 1244
GsuI	CTGGAG	2 cut(s)	904, 1422
HaeII	RGCGCY	1 cut(s)	1532
HaeIII	GGCC	5 cut(s)	225, 356, 1219, 1346, 1711
HapII	CCGG	7 cut(s)	164, 201, 261, 546, 675, 747, 1631
HgaI	GACGC	1 cut(s)	1402
HhaI	GCGC	5 cut(s)	503, 652, 1045, 1240, 1531
Hin1I	GRCGYC	1 cut(s)	456
Hin1II	CATG	4 cut(s)	129, 1538, 1706, 1854
Hin6I	GCGC	5 cut(s)	501, 650, 1043, 1238, 1529
HinP1I	GCGC	5 cut(s)	501, 650, 1043, 1238, 1529
HincII	GTYRAC	2 cut(s)	238, 1464
HindII	GTYRAC	2 cut(s)	238, 1464
HindIII	AAGCTT	1 cut(s)	1208
HinfI	GANTC	5 cut(s)	239, 293, 425, 551, 848
HpaII	CCGG	7 cut(s)	164, 201, 261, 546, 675, 747, 1631
HphI	GGTGA	5 cut(s)	133, 486, 1069, 1124, 1600
Hpy166II	GTNNAC	5 cut(s)	238, 961, 1377, 1464, 1643
Hpy188III	TCNNGA	9 cut(s)	370, 541, 974, 1009, 1369, 1439, 1505, 1655, 1769
Hpy8I	GTNNAC	5 cut(s)	238, 961, 1377, 1464, 1643
Hpy99I	CGWCG	4 cut(s)	239, 458, 461, 560
HpyAV	CCTTC	9 cut(s)	55, 281, 383, 685, 1519, 1566, 1630, 1871, 1899
HpyCH4III	ACNGT	1 cut(s)	1461
HpyCH4IV	ACGT	4 cut(s)	402, 456, 597, 1077
HpyF10VI	GCNNNNNNNGC	8 cut(s)	261, 507, 611, 1216, 1352, 1507, 1708, 1923
HpyF3I	CTNAG	4 cut(s)	653, 789, 1211, 1383
HpySE526I	ACGT	4 cut(s)	402, 456, 597, 1077
Hsp92I	GRCGYC	1 cut(s)	456
Hsp92II	CATG	4 cut(s)	129, 1538, 1706, 1854
HspAI	GCGC	5 cut(s)	501, 650, 1043, 1238, 1529
KroI	GCCGGC	1 cut(s)	746
KroNI	GCCGGC	1 cut(s)	748
Kzo9I	GATC	7 cut(s)	381, 491, 671, 741, 1140, 1203, 1720
LmnI	GCTCC	6 cut(s)	22, 127, 173, 1441, 1536, 1922
Lsp1109I	GCAGC	3 cut(s)	889, 1009, 1833
LweI	GCATC	1 cut(s)	383
MabI	ACCWGGT	1 cut(s)	410
MaeI	CTAG	6 cut(s)	320, 717, 1607, 1655, 1707, 1893
MaeII	ACGT	4 cut(s)	402, 456, 597, 1077
MaeIII	GTNAC	3 cut(s)	1130, 1564, 1880
MalI	GATC	7 cut(s)	383, 493, 673, 743, 1142, 1205, 1722
MbiI	CCGCTC	1 cut(s)	122
MboI	GATC	7 cut(s)	381, 491, 671, 741, 1140, 1203, 1720
MboII	GAAGA	7 cut(s)	43, 694, 805, 902, 1012, 1174, 1611
MflI	RGATCY	2 cut(s)	381, 671
MhlI	GDGCHC	8 cut(s)	170, 333, 536, 632, 1282, 1368, 1406, 1645
MlsI	TGGCCA	1 cut(s)	1219
MluNI	TGGCCA	1 cut(s)	1219
MlyI	GAGTC	4 cut(s)	233, 302, 560, 857
MmeI	TCCRAC	4 cut(s)	415, 538, 706, 1719
Mox20I	TGGCCA	1 cut(s)	1219
MroNI	GCCGGC	1 cut(s)	746
MroXI	GAANNNNTTC	1 cut(s)	1004
MscI	TGGCCA	1 cut(s)	1219
MseI	TTAA	2 cut(s)	876, 882
MslI	CAYNNNNRTG	3 cut(s)	518, 596, 1409
Msp20I	TGGCCA	1 cut(s)	1219
MspA1I	CMGCKG	1 cut(s)	378
MspI	CCGG	7 cut(s)	164, 201, 261, 546, 675, 747, 1631
MspR9I	CCNGG	7 cut(s)	164, 165, 202, 221, 412, 662, 922
MvaI	CCWGG	4 cut(s)	221, 412, 662, 922
MvnI	CGCG	3 cut(s)	650, 1043, 1370
MwoI	GCNNNNNNNGC	8 cut(s)	261, 507, 611, 1216, 1352, 1507, 1708, 1923
NaeI	GCCGGC	1 cut(s)	748
NciI	CCSGG	3 cut(s)	164, 165, 202
NdeII	GATC	7 cut(s)	381, 491, 671, 741, 1140, 1203, 1720
NgoMIV	GCCGGC	1 cut(s)	746
NheI	GCTAGC	1 cut(s)	716
NlaIII	CATG	4 cut(s)	129, 1538, 1706, 1854
NlaIV	GGNNCC	5 cut(s)	169, 206, 416, 673, 1758
NmeAIII	GCCGAG	2 cut(s)	661, 998
NmuCI	GTSAC	1 cut(s)	1130
NruI	TCGCGA	1 cut(s)	1370
NspI	RCATGY	2 cut(s)	1706, 1854
NspV	TTCGAA	2 cut(s)	423, 1875
OliI	CACNNNNGTG	1 cut(s)	1409
PaeI	GCATGC	1 cut(s)	1706
PaqCI	CACCTGC	1 cut(s)	581
PciI	ACATGT	1 cut(s)	1850
PcsI	WCGNNNNNNNCGW	4 cut(s)	240, 584, 618, 1083
PdiI	GCCGGC	1 cut(s)	748
PdmI	GAANNNNTTC	1 cut(s)	1004
PfeI	GAWTC	1 cut(s)	425
PkrI	GCNGC	8 cut(s)	182, 185, 257, 365, 604, 904, 1024, 1823
PleI	GAGTC	4 cut(s)	233, 301, 559, 856
PpsI	GAGTC	4 cut(s)	233, 301, 559, 856
Ppu21I	YACGTR	1 cut(s)	598
PpuMI	RGGWCCY	1 cut(s)	414
PscI	ACATGT	1 cut(s)	1850
PsiI	TTATAA	1 cut(s)	1869
Psp124BI	GAGCTC	2 cut(s)	333, 536
Psp5II	RGGWCCY	1 cut(s)	414
Psp6I	CCWGG	4 cut(s)	219, 410, 660, 920
PspEI	GGTNACC	2 cut(s)	1130, 1880
PspFI	CCCAGC	3 cut(s)	105, 300, 1240
PspGI	CCWGG	4 cut(s)	219, 410, 660, 920
PspN4I	GGNNCC	5 cut(s)	169, 206, 416, 673, 1758
PspPI	GGNCC	3 cut(s)	223, 354, 414
PspPPI	RGGWCCY	1 cut(s)	414
PsuI	RGATCY	2 cut(s)	381, 671
PvuII	CAGCTG	1 cut(s)	378
RruI	TCGCGA	1 cut(s)	1370
RsaI	GTAC	5 cut(s)	212, 248, 584, 865, 962
RsaNI	GTAC	5 cut(s)	211, 247, 583, 864, 961
RseI	CAYNNNNRTG	3 cut(s)	518, 596, 1409
SacI	GAGCTC	2 cut(s)	333, 536
SalI	GTCGAC	1 cut(s)	236
SaqAI	TTAA	2 cut(s)	876, 882
SatI	GCNGC	8 cut(s)	181, 184, 256, 364, 603, 903, 1023, 1822
Sau3AI	GATC	7 cut(s)	381, 491, 671, 741, 1140, 1203, 1720
Sau96I	GGNCC	3 cut(s)	223, 354, 414
ScaI	AGTACT	2 cut(s)	212, 865
SchI	GAGTC	4 cut(s)	233, 302, 560, 857
ScrFI	CCNGG	7 cut(s)	164, 165, 202, 221, 412, 662, 922
SduI	GDGCHC	8 cut(s)	170, 333, 536, 632, 1282, 1368, 1406, 1645
SexAI	ACCWGGT	1 cut(s)	410
SfaNI	GCATC	1 cut(s)	383
SfuI	TTCGAA	2 cut(s)	423, 1875
SinI	GGWCC	1 cut(s)	414
SmaI	CCCGGG	1 cut(s)	165
SmiMI	CAYNNNNRTG	3 cut(s)	518, 596, 1409
SmlI	CTYRAG	1 cut(s)	1505
SmoI	CTYRAG	1 cut(s)	1505
SphI	GCATGC	1 cut(s)	1706
SspMI	CTAG	6 cut(s)	320, 717, 1607, 1655, 1707, 1893
SstI	GAGCTC	2 cut(s)	333, 536
StyD4I	CCNGG	7 cut(s)	162, 163, 200, 219, 410, 660, 920
StyI	CCWWGG	1 cut(s)	1347
TaaI	ACNGT	1 cut(s)	1461
TaiI	ACGT	4 cut(s)	405, 459, 600, 1080
TaqI	TCGA	6 cut(s)	237, 345, 423, 851, 1341, 1875
TaqII	GACCGA	1 cut(s)	635
TatI	WGTACW	3 cut(s)	210, 863, 960
TauI	GCSGC	5 cut(s)	183, 186, 258, 366, 605
TfiI	GAWTC	1 cut(s)	425
Tru1I	TTAA	2 cut(s)	876, 882
Tru9I	TTAA	2 cut(s)	876, 882
TscAI	CASTG	1 cut(s)	1831
TseFI	GTSAC	1 cut(s)	1130
TseI	GCWGC	3 cut(s)	902, 1022, 1821
Tsp45I	GTSAC	1 cut(s)	1130
TspDTI	ATGAA	2 cut(s)	39, 1175
TspGWI	ACGGA	2 cut(s)	544, 1481
TspMI	CCCGGG	1 cut(s)	163
TspRI	CASTG	1 cut(s)	1831
VneI	GTGCAC	1 cut(s)	1641
VpaK11BI	GGWCC	1 cut(s)	414
XagI	CCTNNNNNAGG	1 cut(s)	665
XapI	RAATTY	3 cut(s)	913, 969, 1871
XbaI	TCTAGA	1 cut(s)	1654
XceI	RCATGY	2 cut(s)	1706, 1854
XmaI	CCCGGG	1 cut(s)	163
XmiI	GTMKAC	1 cut(s)	237
XmnI	GAANNNNTTC	1 cut(s)	1004
XspI	CTAG	6 cut(s)	320, 717, 1607, 1655, 1707, 1893
ZraI	GACGTC	1 cut(s)	457
ZrmI	AGTACT	2 cut(s)	212, 865

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.