Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0001738

Physical Location & Seq

Genomic Coordinates Forward (+)

14973590 .. 14980957

Gene Structure 7368 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00175400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2355 bp

ATGGCTTGTTTCTTTCCGACGGTGGGACATGGCGTCATCGAATATGCGGGATGGATTGTGGCGGAGGCGGGTAGATCGCGGCGAGAGGGGATCCCCGTGGGGATCTATTTGTCATGTGTTGGGAGCTGGTTATGGTTGTCGGCGGGATGTAGATATTGTTTGCAACTTGGCAAGGTGGTGCGGCGGTGTCGGGAAGCGGCGAGGCAATGGGGGGATGACGATGGTATTTGCGGCGGCGGGATTGGGTGTCTACGGTGCTTGATTCTTTGGTGGGGGAAGTGGTGTATGGGCGGGGCCCCTGTCTTTGGGCCTTGTGAAAGAGAGCTTCAGAAAATGGGAATGAGGCTGCAAGCTGTTAGTGATGTAGTAAGGTTGAAGTACGAAGTTTCAATAGAGCAAACTCCTCAAAGAGATGAGAAAATTGAGTTTGACAACAAAATATTTATTCTTGTGGTCATATCGCATAATATGTCTGTTCATTGTCACAACTCAAAAGTATACAACTACCATATTCACGGTGTCGTACAGCACCCTAACTTTTTCTCGCCTAAAATTGCACACGTAATAAGATTTTACGAGATGATCGAGCAAATTTCTCTCCCAGCAAAACGTCACCGTTCCGTGCCCGCTCAACTTCACGATCCTGCAGCAGTTCGACCCGGGCTCCAAGCGCCCCCAAATGAACCAGAACATGGAGTGCCAGTACATACTCCAAGGCCTCCGCCTCGTCGATTCCCACTACCTCAAGCTCACCGGCAACTTCCTCCCCCGCCCAACGCCTCCGACTCCTGCTGGTCCGCCTACCAATCCCTCGCCGACAAATTCGTCGCCGACTTCGACATTCGCTCCACCTGCGGCTTCGAGACCTCCTGGATCTCCCAGGGCTGCATGAACATCACTACCAAGGCCCAATTCGAGGACCTCGTCGGCCAAACCGCCCTAGACGGCGTCGTTTCCAACTGCAACCAGTCGCTCGAGAACAACTCCCCCTGCGCCGCCTGCACCACCAGCCTCTCCAGCCTCTCCGCCACCTATTTGACCGGAGAATCCATCGGGAACGTCTCCGGCTGCACCGCCTACCCGTCGATTTACGCCGCCGCGTTTGCCAATCCGTACGGTCCGACTGATCACGGCAACGCCAAGTGCTTCTTTCAGCTCGATTTCACGACGCCCAGCTCCGGTAAGAAGAAAAGGACTCTGATTTTGATTGTGTTGGTTGCTTGCGGTTTCGGATTGTTGGTTTTAATCGGTGGGATTTGGTTCTCATGGCACAAGTACGAAGAGTATATATTCAACAAGAGGCGAAAGGATGCTAATGATAGAATTGAGTCGGGTTTGGGTAATGCATTAGAGTCTATTAGTGGGAGTACTACTTTGGTTAAGTTCACATTCGAAGAGATTAGCAAGGCTACTAAGAATTTCTCTAGAGATAATATAATCGGGAGGGGAGGATATGGGAATGTGTACAAGGGCACTCTGGCTGATGGCTCTGAGGTTGCATTGAAGAGGTTCAAGAATTGCTCTGCTGCCGGTGATGCGAATTTCGCTCACGAGGTTGAGGTGATTGCGAGCGTTAGGCATGTGAATCTTGTTGCTTTGAGAGGTTATTGTACTGCGACGACGCATTTGTTGGCCAGTTCGGCAAAGGATTGCGCTAGGGACTGCGAGGGGTTGGCTTATTTGCATGACGGGGCTCAACCGTCCATCATTCATAGGGATATTAAAGCAAATAACATACTTTTGGATGAGGATTTTGAGGCCAAAGTGGCGGATTTTGGACTGGCAAAGTTTACGCCTGAGGGAATGACGCATTTGAGCACTAGGGTGGCTGGAACGATGGGTTATCTTGCTCCAGAGTATGCATTGTATGGGCAGTTGACAGAGAAAAGTGATGTTTATAGCTTTGGTGTTGTGATGCTTGAGCTTTTGAGTGGAAAGAAAGCACTCCATGTGAACAATGAGGAGCAGCCGTCACTTGTGACGGATTGGGCATGGTCCATGGTGCGAGAAGGAAAAGCGCTGGATGTCATTGAAGATATGCCAGAGAAGGGTCCACCTGAAGTTTTAGAGAAGTATGTACTGGTTGCTGTTCTTTGTTCTCATCCACAGTTGTATGCTAGGCCAACAATGGATCAGGTTGTAAAAATGTTGGAAACAGACTTGTCAGTTCCCTCAATCCCGGAAAGACCAATTCCCCTTGTGGCCGAGATTGATGATATAGAAAGATACAGTAGCAGTGGCTCAATTCAGCTCTCTACTACAGGAGGCTTCCAGACTTATACATTTGAAAGTAGCCTTCCTCGTGACCATAAGGGAGACGGAGAAAGTTCAGGTTCAACAACAGAAGGGAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

784

Amino Acids

86.2

Weight (kDa)

6.26

Isoelectric Point (pI)

52.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SPARK	PF19160	256 - 357	5.3e-23	SPARK
Pkinase	PF00069	475 - 543	3.4e-07	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	477 - 542	1.7e-07	Protein tyrosine and serine/threonine kinase
PK_Tyr_Ser-Thr	PF07714	553 - 717	3.6e-23	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	555 - 715	1.9e-25	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001738)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G48010
fragaria_vesca	FvH4_2g24291 FvH4_2g24291 FvH4_6g05000 FvH4_6g05020
malus_domestica	MD03G1272600.v1.1 MD04G1211500.v1.1 MD11G1293500.v1.1 MD12G1226500.v1.1
prunus_persica	Prupe.6G332100_v2.0.a1 Prupe.6G332200_v2.0.a1 Prupe.8G248800_v2.0.a1
pyrus_communis	pycom03g21710 pycom11g25970
rosa_chinensis	RchiOBHm_Chr3g0453511 RchiOBHm_Chr3g0453521 RchiOBHm_Chr5g0002211 RchiOBHm_Chr6g0292151
rosa_laevigata	RLG00000012057 RLG00000025506 RLG00000025507 RLG00000031008
rosa_multiflora	Rmu_co8460761.1_g000001 Rmu_sc0000510.1_g000020
rosa_roxburghii	Rroxscaffold_1G00073750 Rroxscaffold_2G00125960 Rroxscaffold_6G00430490 Rroxscaffold_7G00175400
rosa_rugosa	Rorug02G0649700 Rorug02G0649800 Rorug06G0223800
rosa_samantha	Rh1BG359400 Rh3AG052600 Rh3AG052700 Rh3BG054100 Rh3BG054200 Rh3CG053400 Rh3CG053500 Rh3DG054200 Rh3DG054300 Rh6AG337200 Rh6BG344400 Rh6CG351200 Rh6DG337400 Rh7BG191800
rosa_wichuraiana	Rw3G004050 Rw3G004060 Rw6G029350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	860
AasI	GACNNNNNNGTC	1 cut(s)	824
Acc36I	ACCTGC	1 cut(s)	860
AccB7I	CCANNNNNTGG	1 cut(s)	692
AccBSI	CCGCTC	1 cut(s)	629
AccI	GTMKAC	2 cut(s)	250, 498
AccII	CGCG	2 cut(s)	79, 1100
AclWI	GGATC	6 cut(s)	85, 98, 110, 635, 881, 2139
AcoI	YGGCCR	3 cut(s)	928, 1632, 2202
AcsI	RAATTY	4 cut(s)	591, 821, 1417, 1540
AcuI	CTGAAG	2 cut(s)	311, 2079
AcyI	GRCGYC	3 cut(s)	33, 948, 1169
AfaI	GTAC	9 cut(s)	380, 525, 705, 1115, 1277, 1369, 1466, 1612, 2079
AfeI	AGCGCT	1 cut(s)	2019
AfiI	CCNNNNNNNGG	6 cut(s)	23, 305, 692, 916, 1178, 2048
AflIII	ACRYGT	1 cut(s)	559
AgsI	TTSAA	8 cut(s)	376, 390, 1294, 1504, 1513, 2033, 2288, 2337
AhdI	GACNNNNNGTC	1 cut(s)	32
AjnI	CCWGG	2 cut(s)	869, 879
AjuI	GAANNNNNNNTTGG	2 cut(s)	896, 928
AleI	CACNNNNGTG	1 cut(s)	1823
AluBI	AGCT	9 cut(s)	126, 325, 353, 749, 1156, 1176, 1902, 1924, 2251
AluI	AGCT	9 cut(s)	126, 325, 353, 749, 1156, 1176, 1902, 1924, 2251
Alw21I	GWGCWC	1 cut(s)	1820
Alw26I	GTCTC	3 cut(s)	857, 1066, 2310
AlwI	GGATC	6 cut(s)	85, 98, 110, 635, 881, 2139
Ama87I	CYCGRG	2 cut(s)	659, 974
Aor51HI	AGCGCT	1 cut(s)	2019
AoxI	GGCC	9 cut(s)	294, 308, 716, 906, 928, 1632, 1758, 2120, 2202
ApaI	GGGCCC	1 cut(s)	298
ApeKI	GCWGC	6 cut(s)	346, 647, 885, 1068, 1526, 1966
ApoI	RAATTY	4 cut(s)	591, 821, 1417, 1540
ArsI	GACNNNNNNTTYG	4 cut(s)	1186, 1218, 1609, 1641
Asp700I	GAANNNNTTC	1 cut(s)	1508
AspLEI	GCGC	4 cut(s)	673, 995, 1655, 2020
AspS9I	GGNCC	9 cut(s)	294, 295, 308, 795, 907, 919, 1118, 1995, 2051
AsuC2I	CCSGG	3 cut(s)	660, 661, 2180
AsuHPI	GGTGA	4 cut(s)	605, 743, 1544, 1573
AsuII	TTCGAA	1 cut(s)	1392
AvaI	CYCGRG	2 cut(s)	659, 974
AvaII	GGWCC	5 cut(s)	795, 919, 1118, 1995, 2051
AxyI	CCTNAGG	1 cut(s)	1797
BaeGI	GKGCMC	3 cut(s)	298, 627, 1475
BalI	TGGCCA	1 cut(s)	1634
BamHI	GGATCC	1 cut(s)	90
BanII	GRGCYC	3 cut(s)	298, 666, 1696
BauI	CACGAG	2 cut(s)	1550, 2301
Bbv12I	GWGCWC	1 cut(s)	1820
BbvI	GCAGC	6 cut(s)	333, 659, 872, 1055, 1513, 1978
BccI	CCATC	6 cut(s)	45, 215, 1058, 1478, 1712, 1831
BceAI	ACGGC	3 cut(s)	961, 1147, 1954
BciT130I	CCWGG	2 cut(s)	871, 881
BclI	TGATCA	1 cut(s)	1126
BcnI	CCSGG	3 cut(s)	660, 661, 2180
BcoDI	GTCTC	3 cut(s)	857, 1066, 2310
BfaI	CTAG	5 cut(s)	941, 1425, 1656, 1821, 2118
BfmI	CTRYAG	2 cut(s)	645, 2259
BfoI	RGCGCY	2 cut(s)	674, 2021
BfuAI	ACCTGC	1 cut(s)	860
BglI	GCCNNNNNGGC	2 cut(s)	1640, 1766
BmcAI	AGTACT	1 cut(s)	1369
Bme1390I	CCNGG	5 cut(s)	660, 661, 871, 881, 2180
Bme18I	GGWCC	5 cut(s)	795, 919, 1118, 1995, 2051
BmeRI	GACNNNNNGTC	1 cut(s)	32
BmeT110I	CYCGRG	2 cut(s)	659, 974
BmgT120I	GGNCC	9 cut(s)	294, 295, 308, 795, 907, 919, 1118, 1995, 2051
BmiI	GGNNCC	6 cut(s)	92, 295, 296, 297, 665, 2052
BmrFI	CCNGG	5 cut(s)	660, 661, 871, 881, 2180
BmsI	GCATC	3 cut(s)	1300, 1525, 1905
BplI	GAGNNNNNCTC	2 cut(s)	968, 1000
BpmI	CTGGAG	2 cut(s)	1000, 1836
Bpu14I	TTCGAA	1 cut(s)	1392
BpuEI	CTTGAG	2 cut(s)	729, 1940
BpuMI	CCSGG	3 cut(s)	660, 661, 2180
BsaAI	YACGTR	1 cut(s)	562
BsaHI	GRCGYC	3 cut(s)	33, 948, 1169
BsaI	GGTCTC	1 cut(s)	857
BsaJI	CCNNGG	7 cut(s)	96, 659, 713, 879, 880, 903, 1998
BsaWI	WCCGGW	2 cut(s)	1040, 1178
BsaXI	ACNNNNNCTCC	8 cut(s)	648, 678, 687, 717, 830, 860, 1955, 1985
Bsc4I	CCNNNNNNNGG	6 cut(s)	23, 305, 692, 916, 1178, 2048
Bse118I	RCCGGY	2 cut(s)	753, 1529
Bse1I	ACTGG	5 cut(s)	701, 967, 1635, 1785, 2085
Bse21I	CCTNAGG	1 cut(s)	1797
Bse3DI	GCAATG	1 cut(s)	212
BseBI	CCWGG	2 cut(s)	871, 881
BseDI	CCNNGG	7 cut(s)	96, 659, 713, 879, 880, 903, 1998
BseGI	GGATG	7 cut(s)	56, 152, 220, 1315, 1750, 2029, 2101
BseLI	CCNNNNNNNGG	6 cut(s)	23, 305, 692, 916, 1178, 2048
BseMI	GCAATG	1 cut(s)	212
BseMII	CTCAG	2 cut(s)	1482, 1788
BseNI	ACTGG	5 cut(s)	701, 967, 1635, 1785, 2085
BseRI	GAGGAG	2 cut(s)	393, 1976
BseSI	GKGCMC	3 cut(s)	298, 627, 1475
BseXI	GCAGC	6 cut(s)	333, 659, 872, 1055, 1513, 1978
BseYI	CCCAGC	2 cut(s)	601, 1172
BsgI	GTGCAG	2 cut(s)	985, 1054
Bsh1236I	CGCG	2 cut(s)	79, 1100
BshFI	GGCC	9 cut(s)	296, 310, 718, 908, 930, 1634, 1760, 2122, 2204
BsiHKAI	GWGCWC	1 cut(s)	1820
BsiHKCI	CYCGRG	2 cut(s)	659, 974
BsiSI	CCGG	7 cut(s)	660, 754, 1041, 1065, 1179, 1530, 2180
BsiWI	CGTACG	1 cut(s)	1113
BslFI	GGGAC	2 cut(s)	39, 1673
BslI	CCNNNNNNNGG	6 cut(s)	23, 305, 692, 916, 1178, 2048
BsmAI	GTCTC	3 cut(s)	857, 1066, 2310
BsmBI	CGTCTC	2 cut(s)	1066, 2310
BsmFI	GGGAC	2 cut(s)	39, 1673
BsnI	GGCC	9 cut(s)	296, 310, 718, 908, 930, 1634, 1760, 2122, 2204
Bso31I	GGTCTC	1 cut(s)	857
BsoBI	CYCGRG	2 cut(s)	659, 974
Bsp119I	TTCGAA	1 cut(s)	1392
Bsp120I	GGGCCC	1 cut(s)	294
Bsp1286I	GDGCHC	6 cut(s)	298, 627, 666, 1475, 1696, 1820
Bsp1407I	TGTACA	1 cut(s)	1464
Bsp143I	GATC	8 cut(s)	74, 90, 102, 582, 640, 873, 1126, 2131
Bsp19I	CCATGG	1 cut(s)	1998
BspANI	GGCC	9 cut(s)	296, 310, 718, 908, 930, 1634, 1760, 2122, 2204
BspCNI	CTCAG	2 cut(s)	1483, 1789
BspFNI	CGCG	2 cut(s)	79, 1100
BspLI	GGNNCC	6 cut(s)	92, 295, 296, 297, 665, 2052
BspMAI	CTGCAG	1 cut(s)	649
BspMI	ACCTGC	1 cut(s)	860
BspPI	GGATC	6 cut(s)	85, 98, 110, 635, 881, 2139
BspT104I	TTCGAA	1 cut(s)	1392
BspTNI	GGTCTC	1 cut(s)	857
BsrBI	CCGCTC	1 cut(s)	629
BsrDI	GCAATG	1 cut(s)	212
BsrFI	RCCGGY	2 cut(s)	753, 1529
BsrGI	TGTACA	1 cut(s)	1464
BsrI	ACTGG	5 cut(s)	701, 967, 1635, 1785, 2085
BssAI	RCCGGY	2 cut(s)	753, 1529
BssECI	CCNNGG	7 cut(s)	96, 659, 713, 879, 880, 903, 1998
BssMI	GATC	8 cut(s)	74, 90, 102, 582, 640, 873, 1126, 2131
BssNAI	GTATAC	1 cut(s)	499
BssNI	GRCGYC	3 cut(s)	33, 948, 1169
BssSI	CACGAG	2 cut(s)	1550, 2301
BssT1I	CCWWGG	3 cut(s)	713, 903, 1998
Bst1107I	GTATAC	1 cut(s)	499
Bst2BI	CACGAG	2 cut(s)	1550, 2301
Bst2UI	CCWGG	2 cut(s)	871, 881
Bst4CI	ACNGT	8 cut(s)	22, 255, 518, 617, 1118, 1701, 2109, 2231
Bst6I	CTCTTC	3 cut(s)	1275, 1389, 1499
BstACI	GRCGYC	3 cut(s)	33, 948, 1169
BstAUI	TGTACA	1 cut(s)	1464
BstBAI	YACGTR	1 cut(s)	562
BstBI	TTCGAA	1 cut(s)	1392
BstC8I	GCNNGC	5 cut(s)	351, 627, 1000, 1222, 1570
BstDEI	CTNAG	3 cut(s)	1413, 1491, 1797
BstDSI	CCRYGG	2 cut(s)	96, 1998
BstF5I	GGATG	7 cut(s)	56, 152, 220, 1315, 1750, 2029, 2101
BstFNI	CGCG	2 cut(s)	79, 1100
BstH2I	RGCGCY	2 cut(s)	674, 2021
BstHHI	GCGC	4 cut(s)	673, 995, 1655, 2020
BstKTI	GATC	8 cut(s)	77, 93, 105, 585, 643, 876, 1129, 2134
BstMAI	GTCTC	3 cut(s)	857, 1066, 2310
BstMBI	GATC	8 cut(s)	74, 90, 102, 582, 640, 873, 1126, 2131
BstNI	CCWGG	2 cut(s)	871, 881
BstNSI	RCATGY	1 cut(s)	1583
BstSCI	CCNGG	5 cut(s)	658, 659, 869, 879, 2178
BstSFI	CTRYAG	2 cut(s)	645, 2259
BstSLI	GKGCMC	3 cut(s)	298, 627, 1475
BstUI	CGCG	2 cut(s)	79, 1100
BstV1I	GCAGC	6 cut(s)	333, 659, 872, 1055, 1513, 1978
BstX2I	RGATCY	3 cut(s)	90, 102, 873
BstYI	RGATCY	3 cut(s)	90, 102, 873
BstZ17I	GTATAC	1 cut(s)	499
Bsu36I	CCTNAGG	1 cut(s)	1797
BsuRI	GGCC	9 cut(s)	296, 310, 718, 908, 930, 1634, 1760, 2122, 2204
BtgI	CCRYGG	2 cut(s)	96, 1998
BtsCI	GGATG	7 cut(s)	56, 152, 220, 1315, 1750, 2029, 2101
BtsI	GCAGTG	1 cut(s)	2242
BtsIMutI	CAGTG	1 cut(s)	2242
BveI	ACCTGC	1 cut(s)	860
Cac8I	GCNNGC	5 cut(s)	351, 627, 1000, 1222, 1570
CfoI	GCGC	4 cut(s)	673, 995, 1655, 2020
Cfr10I	RCCGGY	2 cut(s)	753, 1529
Cfr13I	GGNCC	9 cut(s)	294, 295, 308, 795, 907, 919, 1118, 1995, 2051
Cfr9I	CCCGGG	1 cut(s)	659
CpoI	CGGWCCG	1 cut(s)	1118
CseI	GACGC	5 cut(s)	22, 937, 1177, 1630, 1816
Csp6I	GTAC	9 cut(s)	379, 524, 704, 1114, 1276, 1368, 1465, 1611, 2078
CspI	CGGWCCG	1 cut(s)	1118
CviQI	GTAC	9 cut(s)	379, 524, 704, 1114, 1276, 1368, 1465, 1611, 2078
DdeI	CTNAG	3 cut(s)	1413, 1491, 1797
DpnI	GATC	8 cut(s)	76, 92, 104, 584, 642, 875, 1128, 2133
DpnII	GATC	8 cut(s)	74, 90, 102, 582, 640, 873, 1126, 2131
DrdI	GACNNNNNNGTC	1 cut(s)	824
DriI	GACNNNNNGTC	1 cut(s)	32
DseDI	GACNNNNNNGTC	1 cut(s)	824
EaeI	YGGCCR	3 cut(s)	928, 1632, 2202
Eam1104I	CTCTTC	3 cut(s)	1275, 1389, 1499
Eam1105I	GACNNNNNGTC	1 cut(s)	32
EarI	CTCTTC	3 cut(s)	1275, 1389, 1499
EciI	GGCGGA	5 cut(s)	77, 711, 787, 1015, 1784
Eco130I	CCWWGG	3 cut(s)	713, 903, 1998
Eco147I	AGGCCT	1 cut(s)	718
Eco24I	GRGCYC	3 cut(s)	298, 666, 1696
Eco31I	GGTCTC	1 cut(s)	857
Eco47I	GGWCC	5 cut(s)	795, 919, 1118, 1995, 2051
Eco47III	AGCGCT	1 cut(s)	2019
Eco57I	CTGAAG	2 cut(s)	311, 2079
Eco81I	CCTNAGG	1 cut(s)	1797
Eco88I	CYCGRG	2 cut(s)	659, 974
EcoO109I	RGGNCCY	3 cut(s)	294, 295, 919
EcoRII	CCWGG	2 cut(s)	869, 879
EcoT14I	CCWWGG	3 cut(s)	713, 903, 1998
EcoT22I	ATGCAT	2 cut(s)	1348, 1864
EcoT38I	GRGCYC	3 cut(s)	298, 666, 1696
ErhI	CCWWGG	3 cut(s)	713, 903, 1998
Esp3I	CGTCTC	2 cut(s)	1066, 2310
FalI	AAGNNNNNCTT	2 cut(s)	1133, 1165
FaqI	GGGAC	2 cut(s)	39, 1673
FauI	CCCGC	7 cut(s)	40, 61, 136, 230, 284, 634, 777
FbaI	TGATCA	1 cut(s)	1126
FblI	GTMKAC	2 cut(s)	250, 498
FokI	GGATG	7 cut(s)	63, 159, 227, 1322, 1757, 2036, 2088
FriOI	GRGCYC	3 cut(s)	298, 666, 1696
FspBI	CTAG	5 cut(s)	941, 1425, 1656, 1821, 2118
GlaI	GCGC	4 cut(s)	672, 994, 1654, 2019
GsaI	CCCAGC	2 cut(s)	605, 1176
GsuI	CTGGAG	2 cut(s)	1000, 1836
HaeII	RGCGCY	2 cut(s)	674, 2021
HaeIII	GGCC	9 cut(s)	296, 310, 718, 908, 930, 1634, 1760, 2122, 2204
HapII	CCGG	7 cut(s)	660, 754, 1041, 1065, 1179, 1530, 2180
HgaI	GACGC	5 cut(s)	22, 937, 1177, 1630, 1816
HhaI	GCGC	4 cut(s)	673, 995, 1655, 2020
Hin1I	GRCGYC	3 cut(s)	33, 948, 1169
Hin6I	GCGC	4 cut(s)	671, 993, 1653, 2018
HinP1I	GCGC	4 cut(s)	671, 993, 1653, 2018
HincII	GTYRAC	1 cut(s)	1878
HindII	GTYRAC	1 cut(s)	1878
HinfI	GANTC	8 cut(s)	262, 732, 785, 1046, 1195, 1328, 1352, 1585
HpaII	CCGG	7 cut(s)	660, 754, 1041, 1065, 1179, 1530, 2180
HphI	GGTGA	4 cut(s)	605, 743, 1544, 1573
Hpy166II	GTNNAC	8 cut(s)	251, 499, 1386, 1465, 1791, 1878, 1954, 2054
Hpy188I	TCNGA	7 cut(s)	18, 330, 784, 1122, 1200, 1232, 1492
Hpy8I	GTNNAC	8 cut(s)	251, 499, 1386, 1465, 1791, 1878, 1954, 2054
Hpy99I	CGWCG	9 cut(s)	22, 732, 830, 929, 953, 1087, 1171, 1621, 1624
HpyAV	CCTTC	4 cut(s)	2003, 2041, 2306, 2339
HpyCH4III	ACNGT	8 cut(s)	22, 255, 518, 617, 1118, 1701, 2109, 2231
HpyCH4IV	ACGT	3 cut(s)	561, 610, 1059
HpyF3I	CTNAG	3 cut(s)	1413, 1491, 1797
HpySE526I	ACGT	3 cut(s)	561, 610, 1059
Hsp92I	GRCGYC	3 cut(s)	33, 948, 1169
HspAI	GCGC	4 cut(s)	671, 993, 1653, 2018
Ksp22I	TGATCA	1 cut(s)	1126
Kzo9I	GATC	8 cut(s)	74, 90, 102, 582, 640, 873, 1126, 2131
LmnI	GCTCC	6 cut(s)	123, 669, 851, 1181, 1855, 1963
Lsp1109I	GCAGC	6 cut(s)	333, 659, 872, 1055, 1513, 1978
LweI	GCATC	3 cut(s)	1300, 1525, 1905
MaeI	CTAG	5 cut(s)	941, 1425, 1656, 1821, 2118
MaeII	ACGT	3 cut(s)	561, 610, 1059
MaeIII	GTNAC	5 cut(s)	482, 611, 1971, 1978, 2303
MalI	GATC	8 cut(s)	76, 92, 104, 584, 642, 875, 1128, 2133
MbiI	CCGCTC	1 cut(s)	629
MboI	GATC	8 cut(s)	74, 90, 102, 582, 640, 873, 1126, 2131
MboII	GAAGA	5 cut(s)	1198, 1292, 1406, 1516, 2045
MflI	RGATCY	3 cut(s)	90, 102, 873
MhlI	GDGCHC	6 cut(s)	298, 627, 666, 1475, 1696, 1820
MlsI	TGGCCA	1 cut(s)	1634
MluNI	TGGCCA	1 cut(s)	1634
MlyI	GAGTC	4 cut(s)	779, 1189, 1337, 1361
MmeI	TCCRAC	5 cut(s)	41, 807, 981, 1145, 2130
Mox20I	TGGCCA	1 cut(s)	1634
Mph1103I	ATGCAT	2 cut(s)	1348, 1864
MroXI	GAANNNNTTC	1 cut(s)	1508
MscI	TGGCCA	1 cut(s)	1634
MseI	TTAA	3 cut(s)	1244, 1380, 1722
MslI	CAYNNNNRTG	1 cut(s)	1823
Msp20I	TGGCCA	1 cut(s)	1634
MspI	CCGG	7 cut(s)	660, 754, 1041, 1065, 1179, 1530, 2180
MspR9I	CCNGG	5 cut(s)	660, 661, 871, 881, 2180
MvaI	CCWGG	2 cut(s)	871, 881
MvnI	CGCG	2 cut(s)	79, 1100
NciI	CCSGG	3 cut(s)	660, 661, 2180
NcoI	CCATGG	1 cut(s)	1998
NdeII	GATC	8 cut(s)	74, 90, 102, 582, 640, 873, 1126, 2131
NlaIV	GGNNCC	6 cut(s)	92, 295, 296, 297, 665, 2052
NmeAIII	GCCGAG	1 cut(s)	2230
NmuCI	GTSAC	5 cut(s)	482, 611, 1971, 1978, 2303
NsiI	ATGCAT	2 cut(s)	1348, 1864
NspI	RCATGY	1 cut(s)	1583
NspV	TTCGAA	1 cut(s)	1392
OliI	CACNNNNGTG	1 cut(s)	1823
PaeR7I	CTCGAG	1 cut(s)	974
PaqCI	CACCTGC	1 cut(s)	860
PasI	CCCWGGG	1 cut(s)	880
PceI	AGGCCT	1 cut(s)	718
PcsI	WCGNNNNNNNCGW	3 cut(s)	582, 834, 921
PdmI	GAANNNNTTC	1 cut(s)	1508
PfeI	GAWTC	4 cut(s)	262, 732, 1046, 1585
Pfl23II	CGTACG	1 cut(s)	1113
PflFI	GACNNNGTC	2 cut(s)	923, 947
PflMI	CCANNNNNTGG	1 cut(s)	692
PfoI	TCCNGGA	2 cut(s)	869, 2178
PleI	GAGTC	4 cut(s)	779, 1189, 1336, 1360
PpsI	GAGTC	4 cut(s)	779, 1189, 1336, 1360
Ppu21I	YACGTR	1 cut(s)	562
PpuMI	RGGWCCY	1 cut(s)	919
Psp5II	RGGWCCY	1 cut(s)	919
Psp6I	CCWGG	2 cut(s)	869, 879
PspFI	CCCAGC	2 cut(s)	601, 1172
PspGI	CCWGG	2 cut(s)	869, 879
PspLI	CGTACG	1 cut(s)	1113
PspN4I	GGNNCC	6 cut(s)	92, 295, 296, 297, 665, 2052
PspOMI	GGGCCC	1 cut(s)	294
PspPI	GGNCC	9 cut(s)	294, 295, 308, 795, 907, 919, 1118, 1995, 2051
PspPPI	RGGWCCY	1 cut(s)	919
PstI	CTGCAG	1 cut(s)	649
PsuI	RGATCY	3 cut(s)	90, 102, 873
PsyI	GACNNNGTC	2 cut(s)	923, 947
RsaI	GTAC	9 cut(s)	380, 525, 705, 1115, 1277, 1369, 1466, 1612, 2079
RsaNI	GTAC	9 cut(s)	379, 524, 704, 1114, 1276, 1368, 1465, 1611, 2078
RseI	CAYNNNNRTG	1 cut(s)	1823
Rsr2I	CGGWCCG	1 cut(s)	1118
RsrII	CGGWCCG	1 cut(s)	1118
SaqAI	TTAA	3 cut(s)	1244, 1380, 1722
Sau3AI	GATC	8 cut(s)	74, 90, 102, 582, 640, 873, 1126, 2131
Sau96I	GGNCC	9 cut(s)	294, 295, 308, 795, 907, 919, 1118, 1995, 2051
ScaI	AGTACT	1 cut(s)	1369
SchI	GAGTC	4 cut(s)	779, 1189, 1337, 1361
ScrFI	CCNGG	5 cut(s)	660, 661, 871, 881, 2180
SduI	GDGCHC	6 cut(s)	298, 627, 666, 1475, 1696, 1820
SfaNI	GCATC	3 cut(s)	1300, 1525, 1905
SfcI	CTRYAG	2 cut(s)	645, 2259
Sfr274I	CTCGAG	1 cut(s)	974
SfuI	TTCGAA	1 cut(s)	1392
SinI	GGWCC	5 cut(s)	795, 919, 1118, 1995, 2051
SlaI	CTCGAG	1 cut(s)	974
SmaI	CCCGGG	1 cut(s)	661
SmiMI	CAYNNNNRTG	1 cut(s)	1823
SmlI	CTYRAG	3 cut(s)	744, 974, 1919
SmoI	CTYRAG	3 cut(s)	744, 974, 1919
SseBI	AGGCCT	1 cut(s)	718
SspI	AATATT	1 cut(s)	441
SspMI	CTAG	5 cut(s)	941, 1425, 1656, 1821, 2118
StuI	AGGCCT	1 cut(s)	718
StyD4I	CCNGG	5 cut(s)	658, 659, 869, 879, 2178
StyI	CCWWGG	3 cut(s)	713, 903, 1998
TaaI	ACNGT	8 cut(s)	22, 255, 518, 617, 1118, 1701, 2109, 2231
TaiI	ACGT	3 cut(s)	564, 613, 1062
TatI	WGTACW	5 cut(s)	703, 1367, 1464, 1610, 2077
TauI	GCSGC	9 cut(s)	82, 184, 200, 234, 237, 858, 998, 1097, 1100
TfiI	GAWTC	4 cut(s)	262, 732, 1046, 1585
Tru1I	TTAA	3 cut(s)	1244, 1380, 1722
Tru9I	TTAA	3 cut(s)	1244, 1380, 1722
TscAI	CASTG	1 cut(s)	2242
TseFI	GTSAC	5 cut(s)	482, 611, 1971, 1978, 2303
TseI	GCWGC	6 cut(s)	346, 647, 885, 1068, 1526, 1966
Tsp45I	GTSAC	5 cut(s)	482, 611, 1971, 1978, 2303
TspDTI	ATGAA	4 cut(s)	467, 696, 905, 1700
TspGWI	ACGGA	4 cut(s)	610, 1101, 1997, 2334
TspMI	CCCGGG	1 cut(s)	659
TspRI	CASTG	1 cut(s)	2242
Tth111I	GACNNNGTC	2 cut(s)	923, 947
Van91I	CCANNNNNTGG	1 cut(s)	692
VpaK11BI	GGWCC	5 cut(s)	795, 919, 1118, 1995, 2051
XapI	RAATTY	4 cut(s)	591, 821, 1417, 1540
XbaI	TCTAGA	1 cut(s)	1424
XceI	RCATGY	1 cut(s)	1583
XhoI	CTCGAG	1 cut(s)	974
XmaI	CCCGGG	1 cut(s)	659
XmiI	GTMKAC	2 cut(s)	250, 498
XmnI	GAANNNNTTC	1 cut(s)	1508
XspI	CTAG	5 cut(s)	941, 1425, 1656, 1821, 2118
ZrmI	AGTACT	1 cut(s)	1369
Zsp2I	ATGCAT	2 cut(s)	1348, 1864

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.