Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0001738

Physical Location & Seq

Genomic Coordinates Reverse (-)

53455237 .. 53457152

Gene Structure 1916 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G029350.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1878 bp

ATGTCATCCCGCACCCCCCTCTTCCTCTTACTACTACTCGCTTTACTATCCTTGTCAACTCCAGTTCTCTCCCAGCAAAACGTCACCGTTCCGTGCCCGCTCAACTTCACGATCCTGCAGCAGTTCGACCCGGGCTCGAAGCGCCCCCAAATGAACGATAACATGGAGTGCCAGTACATCCTCCAAGGCCTCCGCCTCGTCGATTCCCACTACCTCCAGCTCACCGGCAACTTCCTCCCCCCGCCCAACGCCTCCGACTCCTGCTGGTCCGCCTACCAAACCCTCGCCGACAAATTCGTCGCCGGCTTCGACATTCGCTCCACCTGCGGCTTCGAGACCTCCTGGATCTCCCAGGGCTGCATGAACATCACCACCAAGGCCCAATTCGAGGACCTCGTCGGCCAAACCGCCCTAGACGGCGTCGTTTCCAACTGCAACCAGTCGCTCGAGAACAACTCCCCCTGCGCCGCCTGCACCACCAGCCTCTCCAGCCTCTCCGCCACCTATTTGACCGGAGAATCCATCGGCAACGTCTCCGGCTGCACCGCCTACCCGTCCATTTACGCCGCCGCGTTCGCCAATCCGTACGGCCCGACCGATCACGGCAACGCCAAGTGCTTCTTTCAGCTCGATTTCACGACAGCCACCTCCGGTAAGAAGAAAAGGACTCTGATTTTGATTGTGTTGGTTGCCTGTGGTTTCGGGTTGTTGATTTTAATCGGTGGGATTTGGTTCTCATGGCACAAGTACGAAGAGCTATATATTCAACAAGAGGCGACAGGATGCTATGATAGAATTGAGTCGGGTTTGGGTAATGCATTAGAGTCCATTAGTGGGAGTACTACTTTGGTTAAGTTCACATTCGAAGAGATTAGCAAGGCTACTAAGAATTTCTCTAGAGATAATATAATCGGGAGGGGAGGATATGGGAATGTGTACAAGGGCACTCTGGCTGATGGCTCTGAAGTTGCATTGAAGAGGTTCAAGAATTGCTCTGCTGCCGGTGACGCGAATTTCACTCACGAGGTTGAGGTGATTGCGAGCGTTAGGCATGTGAATCTTGTTGCTTTGAGAGGTTATTGTACCGCGACGACGCATTTGAATGGGAGTCTCCATGACCATTTGTTTGGGACTTTTGAGAACAAGCTCAGTTGGCCAGTTCGGCAAAGGATTGCGCTAGGGACTGCGAGGGGGTTGGCTTATTTGCATTACGGGGCTCAACCGTCCATCATTCATAGGGATATTAAAGCAAATAACATACTTTTGGATGAGGATTTTGAGGCCAAAGTGGCGGATTTTGGACTTGCAAAGTTTACGCCCGAGGGAATGACACATTTGAGCACTAGGGTGGCTGGAACGATGGGTTATCTTGCTCCGGAGTATGCATTATATGGGCAGCTGACGGAGAAAAGTGATGTTTATAGCTTTGGTGTTGTGATGCTTGAGCTTTTGAGTGGAAAGAAAGCACTCCATGTGAACAATGATAGTCAGCCGTCACTTGTGACGGATTGGGCATGGTCCATGGTGCGAGAAGGAAAAGCGCTGGATGTCATTGAAGATATGCCAGAGAAGGGTTCACCTGAAGTTTTAGAGAAGTATGTATTGGTTGCCGTTCTTTGTTCTCATCCACAGTTGTATGCTAGGCCAACAATGGATCAGGTTGTAAAAATGTTGGAAACAGACTTGTCAGTTCCCTCAATCCCGGAAAGACCAATTCCCCTTGTGGCTGAGATTGATGATATAGAAAGATACAGTAGCAGTGGCTCAATTCAGCTCTCTAGTACAGGAGGCTTCCAGACTTACACATTTGAAAGTAGCCTTCCTTGCGACCATAAGGGAGACGGAGAAAGTTCAGGTTCAACAACAGAAGGGAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

625

Amino Acids

68.13

Weight (kDa)

5.32

Isoelectric Point (pI)

44.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SPARK	PF19160	29 - 181	4.2e-37	SPARK
Pkinase	PF00069	299 - 556	4.5e-38	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	301 - 558	1.5e-36	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001738)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G48010
fragaria_vesca	FvH4_2g24291 FvH4_2g24291 FvH4_6g05000 FvH4_6g05020
malus_domestica	MD03G1272600.v1.1 MD04G1211500.v1.1 MD11G1293500.v1.1 MD12G1226500.v1.1
prunus_persica	Prupe.6G332100_v2.0.a1 Prupe.6G332200_v2.0.a1 Prupe.8G248800_v2.0.a1
pyrus_communis	pycom03g21710 pycom11g25970
rosa_chinensis	RchiOBHm_Chr3g0453511 RchiOBHm_Chr3g0453521 RchiOBHm_Chr5g0002211 RchiOBHm_Chr6g0292151
rosa_laevigata	RLG00000012057 RLG00000025506 RLG00000025507 RLG00000031008
rosa_multiflora	Rmu_co8460761.1_g000001 Rmu_sc0000510.1_g000020
rosa_roxburghii	Rroxscaffold_1G00073750 Rroxscaffold_2G00125960 Rroxscaffold_6G00430490 Rroxscaffold_7G00175400
rosa_rugosa	Rorug02G0649700 Rorug02G0649800 Rorug06G0223800
rosa_samantha	Rh1BG359400 Rh3AG052600 Rh3AG052700 Rh3BG054100 Rh3BG054200 Rh3CG053400 Rh3CG053500 Rh3DG054200 Rh3DG054300 Rh6AG337200 Rh6BG344400 Rh6CG351200 Rh6DG337400 Rh7BG191800
rosa_wichuraiana	Rw3G004050 Rw3G004060 Rw6G029350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	332
AasI	GACNNNNNNGTC	1 cut(s)	296
Acc36I	ACCTGC	1 cut(s)	332
AccBSI	CCGCTC	1 cut(s)	100
AccII	CGCG	3 cut(s)	572, 1010, 1088
AccIII	TCCGGA	1 cut(s)	1375
AclWI	GGATC	3 cut(s)	106, 353, 1662
AcoI	YGGCCR	2 cut(s)	400, 1154
AcsI	RAATTY	3 cut(s)	293, 889, 1012
AcuI	CTGAAG	2 cut(s)	984, 1602
AcyI	GRCGYC	1 cut(s)	420
AfaI	GTAC	7 cut(s)	176, 587, 749, 841, 938, 1084, 1783
AfeI	AGCGCT	1 cut(s)	1542
AfiI	CCNNNNNNNGG	3 cut(s)	388, 834, 1571
AgsI	TTSAA	7 cut(s)	767, 976, 985, 1102, 1556, 1811, 1860
AjnI	CCWGG	2 cut(s)	341, 351
AjuI	GAANNNNNNNTTGG	4 cut(s)	368, 400, 1586, 1618
AleI	CACNNNNGTG	1 cut(s)	1346
AluBI	AGCT	8 cut(s)	220, 628, 757, 1147, 1399, 1425, 1447, 1774
AluI	AGCT	8 cut(s)	220, 628, 757, 1147, 1399, 1425, 1447, 1774
Alw21I	GWGCWC	1 cut(s)	1343
Alw26I	GTCTC	4 cut(s)	329, 538, 1115, 1833
AlwI	GGATC	3 cut(s)	106, 353, 1662
Ama87I	CYCGRG	3 cut(s)	130, 446, 1319
Aor13HI	TCCGGA	1 cut(s)	1375
Aor51HI	AGCGCT	1 cut(s)	1542
AoxI	GGCC	7 cut(s)	187, 378, 400, 589, 1154, 1281, 1643
ApeKI	GCWGC	5 cut(s)	118, 357, 540, 998, 1396
ApoI	RAATTY	3 cut(s)	293, 889, 1012
ArsI	GACNNNNNNTTYG	6 cut(s)	658, 690, 1081, 1109, 1113, 1141
Asp700I	GAANNNNTTC	1 cut(s)	980
AspLEI	GCGC	4 cut(s)	144, 467, 1177, 1543
AspS9I	GGNCC	5 cut(s)	267, 379, 391, 590, 1518
AsuC2I	CCSGG	3 cut(s)	131, 132, 1703
AsuHPI	GGTGA	6 cut(s)	76, 214, 361, 1016, 1045, 1569
AsuII	TTCGAA	1 cut(s)	864
AvaI	CYCGRG	3 cut(s)	130, 446, 1319
AvaII	GGWCC	3 cut(s)	267, 391, 1518
BaeGI	GKGCMC	2 cut(s)	98, 947
BalI	TGGCCA	1 cut(s)	1156
BanII	GRGCYC	2 cut(s)	137, 1219
BauI	CACGAG	1 cut(s)	1022
Bbv12I	GWGCWC	1 cut(s)	1343
BbvI	GCAGC	5 cut(s)	130, 344, 527, 985, 1408
BccI	CCATC	4 cut(s)	530, 950, 1235, 1354
BceAI	ACGGC	5 cut(s)	433, 604, 619, 1477, 1595
BciT130I	CCWGG	2 cut(s)	343, 353
BcnI	CCSGG	3 cut(s)	131, 132, 1703
BcoDI	GTCTC	4 cut(s)	329, 538, 1115, 1833
BfaI	CTAG	6 cut(s)	413, 897, 1178, 1344, 1641, 1779
BfmI	CTRYAG	1 cut(s)	116
BfoI	RGCGCY	2 cut(s)	145, 1544
BfuAI	ACCTGC	1 cut(s)	332
BglI	GCCNNNNNGGC	2 cut(s)	1162, 1289
BisI	GCNGC	9 cut(s)	119, 328, 358, 468, 541, 567, 570, 999, 1397
BlsI	GCNGC	9 cut(s)	120, 329, 359, 469, 542, 568, 571, 1000, 1398
BmcAI	AGTACT	1 cut(s)	841
Bme1390I	CCNGG	5 cut(s)	131, 132, 343, 353, 1703
Bme18I	GGWCC	3 cut(s)	267, 391, 1518
BmeT110I	CYCGRG	3 cut(s)	130, 446, 1319
BmgT120I	GGNCC	5 cut(s)	267, 379, 391, 590, 1518
BmrFI	CCNGG	5 cut(s)	131, 132, 343, 353, 1703
BmsI	GCATC	2 cut(s)	773, 1428
BplI	GAGNNNNNCTC	2 cut(s)	440, 472
BpmI	CTGGAG	3 cut(s)	45, 200, 472
Bpu14I	TTCGAA	1 cut(s)	864
BpuEI	CTTGAG	1 cut(s)	1463
BpuMI	CCSGG	3 cut(s)	131, 132, 1703
BsaBI	GATNNNNATC	1 cut(s)	716
BsaHI	GRCGYC	1 cut(s)	420
BsaI	GGTCTC	1 cut(s)	329
BsaJI	CCNNGG	7 cut(s)	130, 184, 351, 352, 375, 1320, 1521
BsaWI	WCCGGW	3 cut(s)	512, 650, 1375
BsaXI	ACNNNNNCTCC	4 cut(s)	158, 188, 302, 332
Bsc4I	CCNNNNNNNGG	3 cut(s)	388, 834, 1571
Bse118I	RCCGGY	3 cut(s)	224, 302, 1001
Bse1I	ACTGG	4 cut(s)	62, 172, 439, 1157
Bse8I	GATNNNNATC	1 cut(s)	716
BseAI	TCCGGA	1 cut(s)	1375
BseBI	CCWGG	2 cut(s)	343, 353
BseDI	CCNNGG	7 cut(s)	130, 184, 351, 352, 375, 1320, 1521
BseGI	GGATG	6 cut(s)	5, 177, 788, 1273, 1552, 1624
BseJI	GATNNNNATC	1 cut(s)	716
BseLI	CCNNNNNNNGG	3 cut(s)	388, 834, 1571
BseMII	CTCAG	2 cut(s)	1162, 1719
BseNI	ACTGG	4 cut(s)	62, 172, 439, 1157
BseSI	GKGCMC	2 cut(s)	98, 947
BseXI	GCAGC	5 cut(s)	130, 344, 527, 985, 1408
BseYI	CCCAGC	1 cut(s)	72
BsgI	GTGCAG	2 cut(s)	457, 526
Bsh1236I	CGCG	3 cut(s)	572, 1010, 1088
Bsh1285I	CGRYCG	1 cut(s)	597
BshFI	GGCC	7 cut(s)	189, 380, 402, 591, 1156, 1283, 1645
BsiEI	CGRYCG	1 cut(s)	597
BsiHKAI	GWGCWC	1 cut(s)	1343
BsiHKCI	CYCGRG	3 cut(s)	130, 446, 1319
BsiSI	CCGG	9 cut(s)	131, 225, 303, 513, 537, 651, 1002, 1376, 1703
BsiWI	CGTACG	1 cut(s)	585
BslFI	GGGAC	2 cut(s)	1144, 1195
BslI	CCNNNNNNNGG	3 cut(s)	388, 834, 1571
BsmAI	GTCTC	4 cut(s)	329, 538, 1115, 1833
BsmBI	CGTCTC	2 cut(s)	538, 1833
BsmFI	GGGAC	2 cut(s)	1144, 1195
BsnI	GGCC	7 cut(s)	189, 380, 402, 591, 1156, 1283, 1645
Bso31I	GGTCTC	1 cut(s)	329
BsoBI	CYCGRG	3 cut(s)	130, 446, 1319
Bsp119I	TTCGAA	1 cut(s)	864
Bsp1286I	GDGCHC	5 cut(s)	98, 137, 947, 1219, 1343
Bsp13I	TCCGGA	1 cut(s)	1375
Bsp1407I	TGTACA	1 cut(s)	936
Bsp143I	GATC	4 cut(s)	111, 345, 598, 1654
Bsp19I	CCATGG	1 cut(s)	1521
BspANI	GGCC	7 cut(s)	189, 380, 402, 591, 1156, 1283, 1645
BspCNI	CTCAG	2 cut(s)	1161, 1720
BspEI	TCCGGA	1 cut(s)	1375
BspFNI	CGCG	3 cut(s)	572, 1010, 1088
BspMAI	CTGCAG	1 cut(s)	120
BspMI	ACCTGC	1 cut(s)	332
BspPI	GGATC	3 cut(s)	106, 353, 1662
BspQI	GCTCTTC	1 cut(s)	747
BspT104I	TTCGAA	1 cut(s)	864
BspTNI	GGTCTC	1 cut(s)	329
BsrBI	CCGCTC	1 cut(s)	100
BsrFI	RCCGGY	3 cut(s)	224, 302, 1001
BsrGI	TGTACA	1 cut(s)	936
BsrI	ACTGG	4 cut(s)	62, 172, 439, 1157
BssAI	RCCGGY	3 cut(s)	224, 302, 1001
BssECI	CCNNGG	7 cut(s)	130, 184, 351, 352, 375, 1320, 1521
BssMI	GATC	4 cut(s)	111, 345, 598, 1654
BssNI	GRCGYC	1 cut(s)	420
BssSI	CACGAG	1 cut(s)	1022
BssT1I	CCWWGG	3 cut(s)	184, 375, 1521
Bst2BI	CACGAG	1 cut(s)	1022
Bst2UI	CCWGG	2 cut(s)	343, 353
Bst4CI	ACNGT	4 cut(s)	88, 1224, 1632, 1754
Bst6I	CTCTTC	4 cut(s)	26, 747, 861, 971
BstACI	GRCGYC	1 cut(s)	420
BstAUI	TGTACA	1 cut(s)	936
BstBI	TTCGAA	1 cut(s)	864
BstC8I	GCNNGC	4 cut(s)	98, 304, 472, 1042
BstDEI	CTNAG	3 cut(s)	885, 1148, 1728
BstDSI	CCRYGG	1 cut(s)	1521
BstF5I	GGATG	6 cut(s)	5, 177, 788, 1273, 1552, 1624
BstFNI	CGCG	3 cut(s)	572, 1010, 1088
BstH2I	RGCGCY	2 cut(s)	145, 1544
BstHHI	GCGC	4 cut(s)	144, 467, 1177, 1543
BstKTI	GATC	4 cut(s)	114, 348, 601, 1657
BstMAI	GTCTC	4 cut(s)	329, 538, 1115, 1833
BstMBI	GATC	4 cut(s)	111, 345, 598, 1654
BstMCI	CGRYCG	1 cut(s)	597
BstNI	CCWGG	2 cut(s)	343, 353
BstNSI	RCATGY	1 cut(s)	1055
BstSCI	CCNGG	5 cut(s)	129, 130, 341, 351, 1701
BstSFI	CTRYAG	1 cut(s)	116
BstSLI	GKGCMC	2 cut(s)	98, 947
BstUI	CGCG	3 cut(s)	572, 1010, 1088
BstV1I	GCAGC	5 cut(s)	130, 344, 527, 985, 1408
BstX2I	RGATCY	1 cut(s)	345
BstXI	CCANNNNNNTGG	1 cut(s)	1127
BstYI	RGATCY	1 cut(s)	345
BsuRI	GGCC	7 cut(s)	189, 380, 402, 591, 1156, 1283, 1645
BtgI	CCRYGG	1 cut(s)	1521
BtsCI	GGATG	6 cut(s)	5, 177, 788, 1273, 1552, 1624
BtsI	GCAGTG	1 cut(s)	1765
BtsIMutI	CAGTG	1 cut(s)	1765
BveI	ACCTGC	1 cut(s)	332
Cac8I	GCNNGC	4 cut(s)	98, 304, 472, 1042
CfoI	GCGC	4 cut(s)	144, 467, 1177, 1543
Cfr10I	RCCGGY	3 cut(s)	224, 302, 1001
Cfr13I	GGNCC	5 cut(s)	267, 379, 391, 590, 1518
Cfr9I	CCCGGG	1 cut(s)	130
CseI	GACGC	3 cut(s)	409, 1016, 1102
Csp6I	GTAC	7 cut(s)	175, 586, 748, 840, 937, 1083, 1782
CviAII	CATG	8 cut(s)	163, 361, 738, 1052, 1115, 1472, 1515, 1522
CviQI	GTAC	7 cut(s)	175, 586, 748, 840, 937, 1083, 1782
DdeI	CTNAG	3 cut(s)	885, 1148, 1728
DpnI	GATC	4 cut(s)	113, 347, 600, 1656
DpnII	GATC	4 cut(s)	111, 345, 598, 1654
DrdI	GACNNNNNNGTC	1 cut(s)	296
DseDI	GACNNNNNNGTC	1 cut(s)	296
EaeI	YGGCCR	2 cut(s)	400, 1154
Eam1104I	CTCTTC	4 cut(s)	26, 747, 861, 971
EarI	CTCTTC	4 cut(s)	26, 747, 861, 971
EciI	GGCGGA	4 cut(s)	182, 259, 487, 1307
Eco130I	CCWWGG	3 cut(s)	184, 375, 1521
Eco147I	AGGCCT	1 cut(s)	189
Eco24I	GRGCYC	2 cut(s)	137, 1219
Eco31I	GGTCTC	1 cut(s)	329
Eco47I	GGWCC	3 cut(s)	267, 391, 1518
Eco47III	AGCGCT	1 cut(s)	1542
Eco57I	CTGAAG	2 cut(s)	984, 1602
Eco88I	CYCGRG	3 cut(s)	130, 446, 1319
EcoO109I	RGGNCCY	1 cut(s)	391
EcoRII	CCWGG	2 cut(s)	341, 351
EcoT14I	CCWWGG	3 cut(s)	184, 375, 1521
EcoT22I	ATGCAT	2 cut(s)	820, 1387
EcoT38I	GRGCYC	2 cut(s)	137, 1219
ErhI	CCWWGG	3 cut(s)	184, 375, 1521
Esp3I	CGTCTC	2 cut(s)	538, 1833
FaeI	CATG	8 cut(s)	166, 364, 741, 1055, 1118, 1475, 1518, 1525
FalI	AAGNNNNNCTT	2 cut(s)	605, 637
FaqI	GGGAC	2 cut(s)	1144, 1195
FatI	CATG	8 cut(s)	162, 360, 737, 1051, 1114, 1471, 1514, 1521
FauI	CCCGC	3 cut(s)	17, 105, 249
Fnu4HI	GCNGC	9 cut(s)	119, 328, 358, 468, 541, 567, 570, 999, 1397
FokI	GGATG	5 cut(s)	164, 795, 1280, 1559, 1611
FriOI	GRGCYC	2 cut(s)	137, 1219
Fsp4HI	GCNGC	9 cut(s)	119, 328, 358, 468, 541, 567, 570, 999, 1397
FspBI	CTAG	6 cut(s)	413, 897, 1178, 1344, 1641, 1779
GlaI	GCGC	4 cut(s)	143, 466, 1176, 1542
GluI	GCNGC	9 cut(s)	119, 328, 358, 468, 541, 567, 570, 999, 1397
GsaI	CCCAGC	1 cut(s)	76
GsuI	CTGGAG	3 cut(s)	45, 200, 472
HaeII	RGCGCY	2 cut(s)	145, 1544
HaeIII	GGCC	7 cut(s)	189, 380, 402, 591, 1156, 1283, 1645
HapII	CCGG	9 cut(s)	131, 225, 303, 513, 537, 651, 1002, 1376, 1703
HgaI	GACGC	3 cut(s)	409, 1016, 1102
HhaI	GCGC	4 cut(s)	144, 467, 1177, 1543
Hin1I	GRCGYC	1 cut(s)	420
Hin1II	CATG	8 cut(s)	166, 364, 741, 1055, 1118, 1475, 1518, 1525
Hin6I	GCGC	4 cut(s)	142, 465, 1175, 1541
HinP1I	GCGC	4 cut(s)	142, 465, 1175, 1541
HincII	GTYRAC	1 cut(s)	57
HindII	GTYRAC	1 cut(s)	57
HinfI	GANTC	8 cut(s)	203, 257, 518, 667, 800, 824, 1057, 1108
HpaII	CCGG	9 cut(s)	131, 225, 303, 513, 537, 651, 1002, 1376, 1703
HphI	GGTGA	6 cut(s)	76, 214, 361, 1016, 1045, 1569
Hpy166II	GTNNAC	6 cut(s)	57, 858, 937, 1314, 1477, 1577
Hpy188I	TCNGA	3 cut(s)	256, 672, 964
Hpy8I	GTNNAC	6 cut(s)	57, 858, 937, 1314, 1477, 1577
Hpy99I	CGWCG	6 cut(s)	203, 302, 401, 425, 1093, 1096
HpyAV	CCTTC	4 cut(s)	1526, 1564, 1829, 1862
HpyCH4III	ACNGT	4 cut(s)	88, 1224, 1632, 1754
HpyCH4IV	ACGT	2 cut(s)	81, 531
HpyF3I	CTNAG	3 cut(s)	885, 1148, 1728
HpySE526I	ACGT	2 cut(s)	81, 531
Hsp92I	GRCGYC	1 cut(s)	420
Hsp92II	CATG	8 cut(s)	166, 364, 741, 1055, 1118, 1475, 1518, 1525
HspAI	GCGC	4 cut(s)	142, 465, 1175, 1541
Kpn2I	TCCGGA	1 cut(s)	1375
KroI	GCCGGC	1 cut(s)	302
KroNI	GCCGGC	1 cut(s)	304
Kzo9I	GATC	4 cut(s)	111, 345, 598, 1654
LguI	GCTCTTC	1 cut(s)	747
LmnI	GCTCC	2 cut(s)	323, 1378
Lsp1109I	GCAGC	5 cut(s)	130, 344, 527, 985, 1408
LweI	GCATC	2 cut(s)	773, 1428
MaeI	CTAG	6 cut(s)	413, 897, 1178, 1344, 1641, 1779
MaeII	ACGT	2 cut(s)	81, 531
MaeIII	GTNAC	4 cut(s)	82, 1004, 1494, 1501
MalI	GATC	4 cut(s)	113, 347, 600, 1656
MbiI	CCGCTC	1 cut(s)	100
MboI	GATC	4 cut(s)	111, 345, 598, 1654
MboII	GAAGA	6 cut(s)	13, 670, 764, 878, 988, 1568
MflI	RGATCY	1 cut(s)	345
MhlI	GDGCHC	5 cut(s)	98, 137, 947, 1219, 1343
MlsI	TGGCCA	1 cut(s)	1156
MluCI	AATT	8 cut(s)	293, 383, 795, 889, 988, 1012, 1713, 1767
MluNI	TGGCCA	1 cut(s)	1156
MlyI	GAGTC	5 cut(s)	251, 661, 809, 833, 1117
MmeI	TCCRAC	3 cut(s)	279, 453, 1653
Mox20I	TGGCCA	1 cut(s)	1156
Mph1103I	ATGCAT	2 cut(s)	820, 1387
MroI	TCCGGA	1 cut(s)	1375
MroNI	GCCGGC	1 cut(s)	302
MroXI	GAANNNNTTC	1 cut(s)	980
MscI	TGGCCA	1 cut(s)	1156
MseI	TTAA	3 cut(s)	716, 852, 1245
MslI	CAYNNNNRTG	2 cut(s)	1101, 1346
Msp20I	TGGCCA	1 cut(s)	1156
MspA1I	CMGCKG	1 cut(s)	1399
MspI	CCGG	9 cut(s)	131, 225, 303, 513, 537, 651, 1002, 1376, 1703
MspR9I	CCNGG	5 cut(s)	131, 132, 343, 353, 1703
MvaI	CCWGG	2 cut(s)	343, 353
MvnI	CGCG	3 cut(s)	572, 1010, 1088
NaeI	GCCGGC	1 cut(s)	304
NciI	CCSGG	3 cut(s)	131, 132, 1703
NcoI	CCATGG	1 cut(s)	1521
NdeII	GATC	4 cut(s)	111, 345, 598, 1654
NgoMIV	GCCGGC	1 cut(s)	302
NlaIII	CATG	8 cut(s)	166, 364, 741, 1055, 1118, 1475, 1518, 1525
NmuCI	GTSAC	4 cut(s)	82, 1004, 1494, 1501
NsiI	ATGCAT	2 cut(s)	820, 1387
NspI	RCATGY	1 cut(s)	1055
NspV	TTCGAA	1 cut(s)	864
OliI	CACNNNNGTG	1 cut(s)	1346
PaeR7I	CTCGAG	1 cut(s)	446
PaqCI	CACCTGC	1 cut(s)	332
PasI	CCCWGGG	1 cut(s)	352
PceI	AGGCCT	1 cut(s)	189
PciSI	GCTCTTC	1 cut(s)	747
PcsI	WCGNNNNNNNCGW	3 cut(s)	306, 393, 594
PdiI	GCCGGC	1 cut(s)	304
PdmI	GAANNNNTTC	1 cut(s)	980
PfeI	GAWTC	3 cut(s)	203, 518, 1057
Pfl23II	CGTACG	1 cut(s)	585
PflFI	GACNNNGTC	2 cut(s)	395, 419
PfoI	TCCNGGA	2 cut(s)	341, 1701
PkrI	GCNGC	9 cut(s)	120, 329, 359, 469, 542, 568, 571, 1000, 1398
PleI	GAGTC	5 cut(s)	251, 661, 808, 832, 1116
PpsI	GAGTC	5 cut(s)	251, 661, 808, 832, 1116
PpuMI	RGGWCCY	1 cut(s)	391
Psp5II	RGGWCCY	1 cut(s)	391
Psp6I	CCWGG	2 cut(s)	341, 351
PspFI	CCCAGC	1 cut(s)	72
PspGI	CCWGG	2 cut(s)	341, 351
PspLI	CGTACG	1 cut(s)	585
PspPI	GGNCC	5 cut(s)	267, 379, 391, 590, 1518
PspPPI	RGGWCCY	1 cut(s)	391
PstI	CTGCAG	1 cut(s)	120
PsuI	RGATCY	1 cut(s)	345
PsyI	GACNNNGTC	2 cut(s)	395, 419
PvuII	CAGCTG	1 cut(s)	1399
RsaI	GTAC	7 cut(s)	176, 587, 749, 841, 938, 1084, 1783
RsaNI	GTAC	7 cut(s)	175, 586, 748, 840, 937, 1083, 1782
RseI	CAYNNNNRTG	2 cut(s)	1101, 1346
SapI	GCTCTTC	1 cut(s)	747
SaqAI	TTAA	3 cut(s)	716, 852, 1245
SatI	GCNGC	9 cut(s)	119, 328, 358, 468, 541, 567, 570, 999, 1397
Sau3AI	GATC	4 cut(s)	111, 345, 598, 1654
Sau96I	GGNCC	5 cut(s)	267, 379, 391, 590, 1518
ScaI	AGTACT	1 cut(s)	841
SchI	GAGTC	5 cut(s)	251, 661, 809, 833, 1117
ScrFI	CCNGG	5 cut(s)	131, 132, 343, 353, 1703
SduI	GDGCHC	5 cut(s)	98, 137, 947, 1219, 1343
SfaNI	GCATC	2 cut(s)	773, 1428
SfcI	CTRYAG	1 cut(s)	116
Sfr274I	CTCGAG	1 cut(s)	446
SfuI	TTCGAA	1 cut(s)	864
SinI	GGWCC	3 cut(s)	267, 391, 1518
SlaI	CTCGAG	1 cut(s)	446
SmaI	CCCGGG	1 cut(s)	132
SmiMI	CAYNNNNRTG	2 cut(s)	1101, 1346
SmlI	CTYRAG	2 cut(s)	446, 1442
SmoI	CTYRAG	2 cut(s)	446, 1442
Sse9I	AATT	8 cut(s)	293, 383, 795, 889, 988, 1012, 1713, 1767
SseBI	AGGCCT	1 cut(s)	189
SspMI	CTAG	6 cut(s)	413, 897, 1178, 1344, 1641, 1779
StuI	AGGCCT	1 cut(s)	189
StyD4I	CCNGG	5 cut(s)	129, 130, 341, 351, 1701
StyI	CCWWGG	3 cut(s)	184, 375, 1521
TaaI	ACNGT	4 cut(s)	88, 1224, 1632, 1754
TaiI	ACGT	2 cut(s)	84, 534
TaqI	TCGA	9 cut(s)	126, 137, 201, 309, 333, 387, 447, 630, 864
TaqII	GACCGA	1 cut(s)	611
TasI	AATT	8 cut(s)	293, 383, 795, 889, 988, 1012, 1713, 1767
TatI	WGTACW	4 cut(s)	174, 839, 936, 1781
TauI	GCSGC	4 cut(s)	330, 470, 569, 572
TfiI	GAWTC	3 cut(s)	203, 518, 1057
Tru1I	TTAA	3 cut(s)	716, 852, 1245
Tru9I	TTAA	3 cut(s)	716, 852, 1245
TscAI	CASTG	1 cut(s)	1765
TseFI	GTSAC	4 cut(s)	82, 1004, 1494, 1501
TseI	GCWGC	5 cut(s)	118, 357, 540, 998, 1396
Tsp45I	GTSAC	4 cut(s)	82, 1004, 1494, 1501
TspDTI	ATGAA	3 cut(s)	167, 377, 1223
TspGWI	ACGGA	5 cut(s)	81, 573, 1418, 1520, 1857
TspMI	CCCGGG	1 cut(s)	130
TspRI	CASTG	1 cut(s)	1765
Tth111I	GACNNNGTC	2 cut(s)	395, 419
VpaK11BI	GGWCC	3 cut(s)	267, 391, 1518
XapI	RAATTY	3 cut(s)	293, 889, 1012
XbaI	TCTAGA	1 cut(s)	896
XceI	RCATGY	1 cut(s)	1055
XhoI	CTCGAG	1 cut(s)	446
XmaI	CCCGGG	1 cut(s)	130
XmnI	GAANNNNTTC	1 cut(s)	980
XspI	CTAG	6 cut(s)	413, 897, 1178, 1344, 1641, 1779
ZrmI	AGTACT	1 cut(s)	841
Zsp2I	ATGCAT	2 cut(s)	820, 1387

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.