Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0001738

Physical Location & Seq

Genomic Coordinates Reverse (-)

30223128 .. 30224999

Gene Structure 1872 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1226500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1872 bp

ATGAAAACCCTAATGATCTCTCAATTTCTTCAACTCCTTGGATTGGTCTTACTGTTCACTAATTCCAAAGCAATTTCCCATGGAAATTTCCATAGAAAATTGGGCACTGCAACTAAAGCTCCATGCCCATTGAATGTCGACCTTCTCCACAAACTCATCACCCAAAACGCGCATCCATTCCCCTTGCAAGATATTGCGACCGAGTGCCAGTACATGATACAAGGAATCCGTCTCCTTCGATCAGAACATCTTCGAACCACTGGATTGTTTTCGTTGTCTCCAGATGTCCTGGATGCCTGCTGGGATTTGTATGTGAATCTGGTCAATGAATTCATTCCCGGGTTTGATATCATGTCCACATGTGGCTTGAAGACCAATTTCATGTCACAAACTTGTATGAACATCACAACAAAGTCTGATTTCGAAACCTTGGTTTCACGAGATGAGTTGCAACAAATGAAGGGCTCTTGTGATCAGTCTTTGAAGAACAATTCTGCTTGTACTTCCTGCGTGGATTGCTTGTCAAGAGTTCGCGCATCTTATCTCAGAGTCTCGAACGATGGCAATGTTTCGGATTGCAATGGCTACCCGTTTATATATGCTGCAGCATTTTCCAACAGGTTTGGACCAACAGATATAGGGACTGCCAAGTGTTTGTTTCTGCTTGATTTCTCGCCGTCTAATAAAAAGTCCGGAGACCCCAAGGCGGTGATGTGGGGGATCGTGGTTGGTTGTTTTTATGGATTTATCGGGGCATTGGTTGTGGTTTGGTTTCTTTGGATATGGCACAAGAAATGGAAGAGGAAGAAGAGGGAAACTTTGGCTCAAATCAAAGAAGTTTCGAGTGGGGAGATGGAAATTCGCGGTCAAATTAGTACTTCGACAAAGTTCAGCTTCGAGGAAATACAAAAGGCTACGAGGAATTTCTCGAGCGAGAACATAATTGGTTTCGGAGGATATGGGAATGTTTACAAGGGAATGTTGCAAGATGGCTCTGAGGTTGCTTTGAAAAGGTTTAAGAACTGCTCTGCTGCCGGAGACCAAGTTTTCGCACATGAAGTAGAGGTGATTGCAAGCGTAAGGCATGTGAATCTTGTGTCCTTGAGAGGCTATTGTATCGCTACGGTGAATAGAGGAGGCCACCAGAGAATTCTAGCTTGTGAATTGATGCAAAACGGAAGCCTCTATGACCATCTCTTCCAACCGAAATTCAAGAAGCTTAGCTGGTCAATTCGCCAAAAGATTGCGCTTGGAATTGCTCGCGGGTTGGCTTATTTACACCACGGCGTGCAGCCTGCTATCATCCATCGTGACATCAAAGCGAGCAACGTGCTTTTAGATGAAGCATTTGAAGCGAAACTGGCAGATTTTGGCCTTGCAAAGTTCACACCAGAGGGGCAGACACATATGAGCACTAGGGTTGCCGGCACACTAGGTTATGTTGCTCCAGAGTATGCTTTGTATGGCCAAATATCTAAAAAAATCGATGTGTACAGTTTCGGGGTTGTGCTGCTTGAGCTTTTGAGTGGGAAGAAAGCAGTTATAGAATTCAACGACGAGAATAGGCCTTTGTTATTGTCAGATTGGGCTTGGTCACAAGTCAGGGAAGGCAGAGTTTTGGATGTCATTGATGAATCCATGCCCGAATTAGGCCCTCCGGAAGTGAGGGAGAAGTATTCGTTGGTTGCTGTGCTTGCTTCTCATCCAGTTCCAAATGCTAGGCCATCAATGGACCAGATTGTAAAAATCCTGGAAAATGATACCCCAATTCCAACCATCCCTGACCGTCCGTTTGCTCTTTTAGCACATTCTGATGATGCAGAGGGATCAATGAGCTTCAATAGTTTGTGTCTACAAAACAAACACGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko03010 map03010

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

624

Amino Acids

69.42

Weight (kDa)

8.22

Isoelectric Point (pI)

37.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SPARK	PF19160	38 - 193	4.2e-29	SPARK
Pkinase	PF00069	310 - 519	1.7e-41	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	312 - 584	2.3e-39	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001738)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G48010
fragaria_vesca	FvH4_2g24291 FvH4_2g24291 FvH4_6g05000 FvH4_6g05020
malus_domestica	MD03G1272600.v1.1 MD04G1211500.v1.1 MD11G1293500.v1.1 MD12G1226500.v1.1
prunus_persica	Prupe.6G332100_v2.0.a1 Prupe.6G332200_v2.0.a1 Prupe.8G248800_v2.0.a1
pyrus_communis	pycom03g21710 pycom11g25970
rosa_chinensis	RchiOBHm_Chr3g0453511 RchiOBHm_Chr3g0453521 RchiOBHm_Chr5g0002211 RchiOBHm_Chr6g0292151
rosa_laevigata	RLG00000012057 RLG00000025506 RLG00000025507 RLG00000031008
rosa_multiflora	Rmu_co8460761.1_g000001 Rmu_sc0000510.1_g000020
rosa_roxburghii	Rroxscaffold_1G00073750 Rroxscaffold_2G00125960 Rroxscaffold_6G00430490 Rroxscaffold_7G00175400
rosa_rugosa	Rorug02G0649700 Rorug02G0649800 Rorug06G0223800
rosa_samantha	Rh1BG359400 Rh3AG052600 Rh3AG052700 Rh3BG054100 Rh3BG054200 Rh3CG053400 Rh3CG053500 Rh3DG054200 Rh3DG054300 Rh6AG337200 Rh6BG344400 Rh6CG351200 Rh6DG337400 Rh7BG191800
rosa_wichuraiana	Rw3G004050 Rw3G004060 Rw6G029350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	2 cut(s)	138, 1852
AccII	CGCG	4 cut(s)	170, 534, 864, 1263
AccIII	TCCGGA	2 cut(s)	692, 1657
AciI	CCGC	3 cut(s)	707, 864, 1263
AclWI	GGATC	2 cut(s)	728, 1834
AcoI	YGGCCR	1 cut(s)	1465
AcsI	RAATTY	7 cut(s)	85, 329, 858, 922, 1149, 1208, 1547
AdeI	CACNNNGTG	1 cut(s)	1288
AfaI	GTAC	4 cut(s)	212, 502, 877, 1493
AfiI	CCNNNNNNNGG	3 cut(s)	43, 706, 1649
AflIII	ACRYGT	1 cut(s)	359
AgsI	TTSAA	9 cut(s)	32, 133, 370, 484, 1009, 1213, 1352, 1552, 1840
AjnI	CCWGG	2 cut(s)	288, 1749
AjuI	GAANNNNNNNTTGG	2 cut(s)	1664, 1696
AluBI	AGCT	7 cut(s)	119, 894, 1157, 1219, 1224, 1519, 1836
AluI	AGCT	7 cut(s)	119, 894, 1157, 1219, 1224, 1519, 1836
Alw21I	GWGCWC	1 cut(s)	1415
Alw26I	GTCTC	5 cut(s)	236, 282, 556, 690, 1032
AlwI	GGATC	2 cut(s)	728, 1834
Ama87I	CYCGRG	2 cut(s)	338, 928
Aor13HI	TCCGGA	2 cut(s)	692, 1657
AoxI	GGCC	6 cut(s)	1138, 1372, 1465, 1565, 1651, 1721
ApeKI	GCWGC	5 cut(s)	602, 605, 1031, 1291, 1510
ApoI	RAATTY	7 cut(s)	85, 329, 858, 922, 1149, 1208, 1547
ArsI	GACNNNNNNTTYG	4 cut(s)	1031, 1063, 1775, 1807
Asp700I	GAANNNNTTC	2 cut(s)	249, 333
AspLEI	GCGC	3 cut(s)	172, 536, 1249
AspS9I	GGNCC	3 cut(s)	626, 1652, 1732
AsuC2I	CCSGG	2 cut(s)	339, 340
AsuHPI	GGTGA	4 cut(s)	151, 721, 1078, 1138
AsuII	TTCGAA	2 cut(s)	253, 423
AvaI	CYCGRG	2 cut(s)	338, 928
AvaII	GGWCC	2 cut(s)	626, 1732
BaeGI	GKGCMC	1 cut(s)	107
BalI	TGGCCA	1 cut(s)	1467
BanII	GRGCYC	1 cut(s)	467
BauI	CACGAG	1 cut(s)	438
BbsI	GAAGAC	1 cut(s)	377
Bbv12I	GWGCWC	1 cut(s)	1415
BbvI	GCAGC	5 cut(s)	589, 617, 1018, 1303, 1497
BccI	CCATC	7 cut(s)	554, 847, 983, 1200, 1314, 1732, 1784
BceAI	ACGGC	2 cut(s)	661, 1300
BcgI	CGANNNNNNTGC	2 cut(s)	1312, 1346
BciT130I	CCWGG	2 cut(s)	290, 1751
BclI	TGATCA	1 cut(s)	472
BcnI	CCSGG	2 cut(s)	339, 340
BcoDI	GTCTC	5 cut(s)	236, 282, 556, 690, 1032
BfaI	CTAG	4 cut(s)	1154, 1416, 1433, 1719
BfmI	CTRYAG	1 cut(s)	603
BisI	GCNGC	5 cut(s)	603, 606, 1032, 1292, 1511
BlpI	GCTNAGC	1 cut(s)	1220
BlsI	GCNGC	5 cut(s)	604, 607, 1033, 1293, 1512
BmcAI	AGTACT	1 cut(s)	877
Bme1390I	CCNGG	4 cut(s)	290, 339, 340, 1751
Bme18I	GGWCC	2 cut(s)	626, 1732
BmeT110I	CYCGRG	2 cut(s)	338, 928
BmgT120I	GGNCC	3 cut(s)	626, 1652, 1732
BmrFI	CCNGG	4 cut(s)	290, 339, 340, 1751
BmsI	GCATC	5 cut(s)	181, 283, 545, 1158, 1807
BpiI	GAAGAC	1 cut(s)	377
BpmI	CTGGAG	2 cut(s)	264, 1431
Bpu1102I	GCTNAGC	1 cut(s)	1220
Bpu14I	TTCGAA	2 cut(s)	253, 423
BpuEI	CTTGAG	2 cut(s)	1123, 1535
BpuMI	CCSGG	2 cut(s)	339, 340
Bsa29I	ATCGAT	1 cut(s)	1485
BsaI	GGTCTC	2 cut(s)	690, 1032
BsaJI	CCNNGG	6 cut(s)	37, 79, 338, 429, 702, 1282
BsaWI	WCCGGW	2 cut(s)	692, 1657
BsaXI	ACNNNNNCTCC	4 cut(s)	103, 133, 1029, 1059
Bsc4I	CCNNNNNNNGG	3 cut(s)	43, 706, 1649
Bse118I	RCCGGY	1 cut(s)	1424
Bse1I	ACTGG	4 cut(s)	208, 265, 1365, 1706
Bse3DI	GCAATG	2 cut(s)	571, 586
BseAI	TCCGGA	2 cut(s)	692, 1657
BseBI	CCWGG	2 cut(s)	290, 1751
BseCI	ATCGAT	1 cut(s)	1485
BseDI	CCNNGG	6 cut(s)	37, 79, 338, 429, 702, 1282
BseGI	GGATG	6 cut(s)	172, 298, 1302, 1627, 1702, 1776
BseLI	CCNNNNNNNGG	3 cut(s)	43, 706, 1649
BseMI	GCAATG	2 cut(s)	571, 586
BseMII	CTCAG	2 cut(s)	559, 987
BseNI	ACTGG	4 cut(s)	208, 265, 1365, 1706
BseRI	GAGGAG	1 cut(s)	1149
BseSI	GKGCMC	1 cut(s)	107
BseXI	GCAGC	5 cut(s)	589, 617, 1018, 1303, 1497
BseYI	CCCAGC	1 cut(s)	300
BsgI	GTGCAG	1 cut(s)	1310
Bsh1236I	CGCG	4 cut(s)	170, 534, 864, 1263
Bsh1285I	CGRYCG	1 cut(s)	201
BshFI	GGCC	6 cut(s)	1140, 1374, 1467, 1567, 1653, 1723
BshVI	ATCGAT	1 cut(s)	1485
BsiEI	CGRYCG	1 cut(s)	201
BsiHKAI	GWGCWC	1 cut(s)	1415
BsiHKCI	CYCGRG	2 cut(s)	338, 928
BsiSI	CCGG	5 cut(s)	339, 693, 1035, 1425, 1658
BslFI	GGGAC	1 cut(s)	655
BslI	CCNNNNNNNGG	3 cut(s)	43, 706, 1649
BsmAI	GTCTC	5 cut(s)	236, 282, 556, 690, 1032
BsmBI	CGTCTC	1 cut(s)	236
BsmFI	GGGAC	1 cut(s)	655
BsnI	GGCC	6 cut(s)	1140, 1374, 1467, 1567, 1653, 1723
Bso31I	GGTCTC	2 cut(s)	690, 1032
BsoBI	CYCGRG	2 cut(s)	338, 928
Bsp119I	TTCGAA	2 cut(s)	253, 423
Bsp1286I	GDGCHC	3 cut(s)	107, 467, 1415
Bsp13I	TCCGGA	2 cut(s)	692, 1657
Bsp1407I	TGTACA	1 cut(s)	1491
Bsp143I	GATC	5 cut(s)	15, 239, 472, 720, 1826
Bsp1720I	GCTNAGC	1 cut(s)	1220
Bsp19I	CCATGG	1 cut(s)	79
BspACI	CCGC	3 cut(s)	707, 864, 1263
BspANI	GGCC	6 cut(s)	1140, 1374, 1467, 1567, 1653, 1723
BspCNI	CTCAG	2 cut(s)	558, 988
BspDI	ATCGAT	1 cut(s)	1485
BspEI	TCCGGA	2 cut(s)	692, 1657
BspFNI	CGCG	4 cut(s)	170, 534, 864, 1263
BspMAI	CTGCAG	1 cut(s)	607
BspPI	GGATC	2 cut(s)	728, 1834
BspT104I	TTCGAA	2 cut(s)	253, 423
BspTNI	GGTCTC	2 cut(s)	690, 1032
BsrDI	GCAATG	2 cut(s)	571, 586
BsrFI	RCCGGY	1 cut(s)	1424
BsrGI	TGTACA	1 cut(s)	1491
BsrI	ACTGG	4 cut(s)	208, 265, 1365, 1706
BssAI	RCCGGY	1 cut(s)	1424
BssECI	CCNNGG	6 cut(s)	37, 79, 338, 429, 702, 1282
BssMI	GATC	5 cut(s)	15, 239, 472, 720, 1826
BssSI	CACGAG	1 cut(s)	438
BssT1I	CCWWGG	4 cut(s)	37, 79, 429, 702
Bst2BI	CACGAG	1 cut(s)	438
Bst2UI	CCWGG	2 cut(s)	290, 1751
Bst4CI	ACNGT	4 cut(s)	54, 1126, 1496, 1787
Bst6I	CTCTTC	3 cut(s)	794, 803, 1202
BstAUI	TGTACA	1 cut(s)	1491
BstBI	TTCGAA	2 cut(s)	253, 423
BstC8I	GCNNGC	8 cut(s)	298, 1075, 1261, 1289, 1296, 1324, 1426, 1695
BstDEI	CTNAG	3 cut(s)	545, 996, 1220
BstDSI	CCRYGG	2 cut(s)	79, 1282
BstF5I	GGATG	6 cut(s)	172, 298, 1302, 1627, 1702, 1776
BstFNI	CGCG	4 cut(s)	170, 534, 864, 1263
BstHHI	GCGC	3 cut(s)	172, 536, 1249
BstKTI	GATC	5 cut(s)	18, 242, 475, 723, 1829
BstMAI	GTCTC	5 cut(s)	236, 282, 556, 690, 1032
BstMBI	GATC	5 cut(s)	15, 239, 472, 720, 1826
BstMCI	CGRYCG	1 cut(s)	201
BstMWI	GCNNNNNNNGC	7 cut(s)	116, 516, 1352, 1361, 1516, 1694, 1802
BstNI	CCWGG	2 cut(s)	290, 1751
BstNSI	RCATGY	2 cut(s)	363, 1088
BstSCI	CCNGG	4 cut(s)	288, 337, 338, 1749
BstSFI	CTRYAG	1 cut(s)	603
BstSLI	GKGCMC	1 cut(s)	107
BstUI	CGCG	4 cut(s)	170, 534, 864, 1263
BstV1I	GCAGC	5 cut(s)	589, 617, 1018, 1303, 1497
BstV2I	GAAGAC	1 cut(s)	377
Bsu15I	ATCGAT	1 cut(s)	1485
BsuRI	GGCC	6 cut(s)	1140, 1374, 1467, 1567, 1653, 1723
BsuTUI	ATCGAT	1 cut(s)	1485
BtgI	CCRYGG	2 cut(s)	79, 1282
BtsCI	GGATG	6 cut(s)	172, 298, 1302, 1627, 1702, 1776
BtsI	GCAGTG	1 cut(s)	105
BtsIMutI	CAGTG	2 cut(s)	105, 258
Cac8I	GCNNGC	8 cut(s)	298, 1075, 1261, 1289, 1296, 1324, 1426, 1695
CfoI	GCGC	3 cut(s)	172, 536, 1249
Cfr10I	RCCGGY	1 cut(s)	1424
Cfr13I	GGNCC	3 cut(s)	626, 1652, 1732
Cfr9I	CCCGGG	1 cut(s)	338
ClaI	ATCGAT	1 cut(s)	1485
Csp6I	GTAC	4 cut(s)	211, 501, 876, 1492
CviAII	CATG	9 cut(s)	80, 123, 214, 352, 360, 382, 1055, 1085, 1639
CviQI	GTAC	4 cut(s)	211, 501, 876, 1492
DdeI	CTNAG	3 cut(s)	545, 996, 1220
DpnI	GATC	5 cut(s)	17, 241, 474, 722, 1828
DpnII	GATC	5 cut(s)	15, 239, 472, 720, 1826
DraIII	CACNNNGTG	1 cut(s)	1288
EaeI	YGGCCR	1 cut(s)	1465
Eam1104I	CTCTTC	3 cut(s)	794, 803, 1202
EarI	CTCTTC	3 cut(s)	794, 803, 1202
Eco130I	CCWWGG	4 cut(s)	37, 79, 429, 702
Eco147I	AGGCCT	1 cut(s)	1567
Eco24I	GRGCYC	1 cut(s)	467
Eco31I	GGTCTC	2 cut(s)	690, 1032
Eco32I	GATATC	1 cut(s)	349
Eco47I	GGWCC	2 cut(s)	626, 1732
Eco88I	CYCGRG	2 cut(s)	338, 928
EcoO109I	RGGNCCY	1 cut(s)	1652
EcoRI	GAATTC	3 cut(s)	329, 1149, 1547
EcoRII	CCWGG	2 cut(s)	288, 1749
EcoRV	GATATC	1 cut(s)	349
EcoT14I	CCWWGG	4 cut(s)	37, 79, 429, 702
EcoT38I	GRGCYC	1 cut(s)	467
ErhI	CCWWGG	4 cut(s)	37, 79, 429, 702
Esp3I	CGTCTC	1 cut(s)	236
FaeI	CATG	9 cut(s)	83, 126, 217, 355, 363, 385, 1058, 1088, 1642
FalI	AAGNNNNNCTT	2 cut(s)	878, 910
FaqI	GGGAC	1 cut(s)	655
FatI	CATG	9 cut(s)	79, 122, 213, 351, 359, 381, 1054, 1084, 1638
FauI	CCCGC	1 cut(s)	1256
FauNDI	CATATG	1 cut(s)	1407
FbaI	TGATCA	1 cut(s)	472
FblI	GTMKAC	2 cut(s)	138, 1852
Fnu4HI	GCNGC	5 cut(s)	603, 606, 1032, 1292, 1511
FokI	GGATG	6 cut(s)	159, 305, 1289, 1634, 1689, 1763
FriOI	GRGCYC	1 cut(s)	467
Fsp4HI	GCNGC	5 cut(s)	603, 606, 1032, 1292, 1511
FspBI	CTAG	4 cut(s)	1154, 1416, 1433, 1719
GlaI	GCGC	3 cut(s)	171, 535, 1248
GluI	GCNGC	5 cut(s)	603, 606, 1032, 1292, 1511
GsaI	CCCAGC	1 cut(s)	304
GsuI	CTGGAG	2 cut(s)	264, 1431
HaeIII	GGCC	6 cut(s)	1140, 1374, 1467, 1567, 1653, 1723
HapII	CCGG	5 cut(s)	339, 693, 1035, 1425, 1658
HhaI	GCGC	3 cut(s)	172, 536, 1249
Hin1II	CATG	9 cut(s)	83, 126, 217, 355, 363, 385, 1058, 1088, 1642
Hin6I	GCGC	3 cut(s)	170, 534, 1247
HinP1I	GCGC	3 cut(s)	170, 534, 1247
HincII	GTYRAC	1 cut(s)	139
HindII	GTYRAC	1 cut(s)	139
HindIII	AAGCTT	1 cut(s)	1217
HinfI	GANTC	5 cut(s)	225, 316, 549, 1090, 1634
HpaII	CCGG	5 cut(s)	339, 693, 1035, 1425, 1658
HphI	GGTGA	4 cut(s)	151, 721, 1078, 1138
Hpy166II	GTNNAC	7 cut(s)	57, 139, 357, 970, 1386, 1492, 1853
Hpy188I	TCNGA	8 cut(s)	244, 418, 548, 574, 953, 997, 1582, 1813
Hpy8I	GTNNAC	7 cut(s)	57, 139, 357, 970, 1386, 1492, 1853
Hpy99I	CGWCG	1 cut(s)	1559
HpyAV	CCTTC	4 cut(s)	152, 245, 454, 1601
HpyCH4III	ACNGT	4 cut(s)	54, 1126, 1496, 1787
HpyCH4IV	ACGT	1 cut(s)	1329
HpyF10VI	GCNNNNNNNGC	7 cut(s)	116, 516, 1352, 1361, 1516, 1694, 1802
HpyF3I	CTNAG	3 cut(s)	545, 996, 1220
HpySE526I	ACGT	1 cut(s)	1329
Hsp92II	CATG	9 cut(s)	83, 126, 217, 355, 363, 385, 1058, 1088, 1642
HspAI	GCGC	3 cut(s)	170, 534, 1247
Kpn2I	TCCGGA	2 cut(s)	692, 1657
KroI	GCCGGC	1 cut(s)	1424
KroNI	GCCGGC	1 cut(s)	1426
Ksp22I	TGATCA	1 cut(s)	472
Kzo9I	GATC	5 cut(s)	15, 239, 472, 720, 1826
LmnI	GCTCC	2 cut(s)	124, 1450
Lsp1109I	GCAGC	5 cut(s)	589, 617, 1018, 1303, 1497
LweI	GCATC	5 cut(s)	181, 283, 545, 1158, 1807
MaeI	CTAG	4 cut(s)	1154, 1416, 1433, 1719
MaeII	ACGT	1 cut(s)	1329
MaeIII	GTNAC	3 cut(s)	384, 1310, 1593
MalI	GATC	5 cut(s)	17, 241, 474, 722, 1828
MboI	GATC	5 cut(s)	15, 239, 472, 720, 1826
MboII	GAAGA	9 cut(s)	20, 242, 382, 496, 811, 817, 820, 1189, 1543
MhlI	GDGCHC	3 cut(s)	107, 467, 1415
MlsI	TGGCCA	1 cut(s)	1467
MluNI	TGGCCA	1 cut(s)	1467
MlyI	GAGTC	1 cut(s)	558
MmeI	TCCRAC	3 cut(s)	639, 1225, 1796
Mox20I	TGGCCA	1 cut(s)	1467
MroI	TCCGGA	2 cut(s)	692, 1657
MroNI	GCCGGC	1 cut(s)	1424
MroXI	GAANNNNTTC	2 cut(s)	249, 333
MscI	TGGCCA	1 cut(s)	1467
MseI	TTAA	1 cut(s)	1017
MslI	CAYNNNNRTG	1 cut(s)	1812
Msp20I	TGGCCA	1 cut(s)	1467
MspI	CCGG	5 cut(s)	339, 693, 1035, 1425, 1658
MspR9I	CCNGG	4 cut(s)	290, 339, 340, 1751
MvaI	CCWGG	2 cut(s)	290, 1751
MvnI	CGCG	4 cut(s)	170, 534, 864, 1263
MwoI	GCNNNNNNNGC	7 cut(s)	116, 516, 1352, 1361, 1516, 1694, 1802
NaeI	GCCGGC	1 cut(s)	1426
NciI	CCSGG	2 cut(s)	339, 340
NcoI	CCATGG	1 cut(s)	79
NdeI	CATATG	1 cut(s)	1407
NdeII	GATC	5 cut(s)	15, 239, 472, 720, 1826
NgoMIV	GCCGGC	1 cut(s)	1424
NlaIII	CATG	9 cut(s)	83, 126, 217, 355, 363, 385, 1058, 1088, 1642
NmuCI	GTSAC	3 cut(s)	384, 1310, 1593
NspI	RCATGY	2 cut(s)	363, 1088
NspV	TTCGAA	2 cut(s)	253, 423
PaeR7I	CTCGAG	1 cut(s)	928
PceI	AGGCCT	1 cut(s)	1567
PciI	ACATGT	1 cut(s)	359
PdiI	GCCGGC	1 cut(s)	1426
PdmI	GAANNNNTTC	2 cut(s)	249, 333
PfeI	GAWTC	4 cut(s)	225, 316, 1090, 1634
PfoI	TCCNGGA	2 cut(s)	288, 1749
PkrI	GCNGC	5 cut(s)	604, 607, 1033, 1293, 1512
PleI	GAGTC	1 cut(s)	557
PpsI	GAGTC	1 cut(s)	557
PscI	ACATGT	1 cut(s)	359
Psp6I	CCWGG	2 cut(s)	288, 1749
PspFI	CCCAGC	1 cut(s)	300
PspGI	CCWGG	2 cut(s)	288, 1749
PspPI	GGNCC	3 cut(s)	626, 1652, 1732
PstI	CTGCAG	1 cut(s)	607
RsaI	GTAC	4 cut(s)	212, 502, 877, 1493
RsaNI	GTAC	4 cut(s)	211, 501, 876, 1492
RseI	CAYNNNNRTG	1 cut(s)	1812
SalI	GTCGAC	1 cut(s)	137
SaqAI	TTAA	1 cut(s)	1017
SatI	GCNGC	5 cut(s)	603, 606, 1032, 1292, 1511
Sau3AI	GATC	5 cut(s)	15, 239, 472, 720, 1826
Sau96I	GGNCC	3 cut(s)	626, 1652, 1732
ScaI	AGTACT	1 cut(s)	877
SchI	GAGTC	1 cut(s)	558
ScrFI	CCNGG	4 cut(s)	290, 339, 340, 1751
SduI	GDGCHC	3 cut(s)	107, 467, 1415
SfaNI	GCATC	5 cut(s)	181, 283, 545, 1158, 1807
SfcI	CTRYAG	1 cut(s)	603
Sfr274I	CTCGAG	1 cut(s)	928
SfuI	TTCGAA	2 cut(s)	253, 423
SinI	GGWCC	2 cut(s)	626, 1732
SlaI	CTCGAG	1 cut(s)	928
SmaI	CCCGGG	1 cut(s)	340
SmiMI	CAYNNNNRTG	1 cut(s)	1812
SmlI	CTYRAG	3 cut(s)	928, 1102, 1514
SmoI	CTYRAG	3 cut(s)	928, 1102, 1514
SseBI	AGGCCT	1 cut(s)	1567
SsiI	CCGC	3 cut(s)	707, 864, 1263
SspMI	CTAG	4 cut(s)	1154, 1416, 1433, 1719
StuI	AGGCCT	1 cut(s)	1567
StyD4I	CCNGG	4 cut(s)	288, 337, 338, 1749
StyI	CCWWGG	4 cut(s)	37, 79, 429, 702
TaaI	ACNGT	4 cut(s)	54, 1126, 1496, 1787
TaiI	ACGT	1 cut(s)	1332
TaqII	GACCGA	1 cut(s)	215
TatI	WGTACW	4 cut(s)	210, 500, 875, 1491
TfiI	GAWTC	4 cut(s)	225, 316, 1090, 1634
Tru1I	TTAA	1 cut(s)	1017
Tru9I	TTAA	1 cut(s)	1017
TscAI	CASTG	2 cut(s)	112, 265
TseFI	GTSAC	3 cut(s)	384, 1310, 1593
TseI	GCWGC	5 cut(s)	602, 605, 1031, 1291, 1510
Tsp45I	GTSAC	3 cut(s)	384, 1310, 1593
TspDTI	ATGAA	9 cut(s)	17, 322, 342, 370, 413, 473, 1071, 1356, 1647
TspGWI	ACGGA	3 cut(s)	218, 1191, 1779
TspMI	CCCGGG	1 cut(s)	338
TspRI	CASTG	2 cut(s)	112, 265
VpaK11BI	GGWCC	2 cut(s)	626, 1732
XapI	RAATTY	7 cut(s)	85, 329, 858, 922, 1149, 1208, 1547
XceI	RCATGY	2 cut(s)	363, 1088
XhoI	CTCGAG	1 cut(s)	928
XmaI	CCCGGG	1 cut(s)	338
XmiI	GTMKAC	2 cut(s)	138, 1852
XmnI	GAANNNNTTC	2 cut(s)	249, 333
XspI	CTAG	4 cut(s)	1154, 1416, 1433, 1719
ZrmI	AGTACT	1 cut(s)	877

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.