Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6A

Orthogroup ID

OG0001738

Physical Location & Seq

Genomic Coordinates Reverse (-)

53754643 .. 53757410

Gene Structure 2768 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6AG337200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1917 bp

ATGTCATCCCGCACCCCCCTCTTGCTCTTGCTACTACTCGCTATACTATCCTTGTCAACTCGAGTTCTCTCCCAGCAAAACGTCACCGTTCCGTGCCCGCTCAACTTCACGATCCTGCAGCAGTTCGACCCCGGCTCCAAGCGCCCCCAAATGAACGATAACATGGAGTGCCAGTACATCCTCCAAGGCCTCCGCCTCGTCGATTCCCACTACCTCCAGCTCACCGGCAACTTCCTCCCCCCGCCCAACGCCTCCGACTCCTGCTGGTCCGCCTACCAAACCCTCGCCGACAAATTCGTCGCCGGCTTCGACATTCGCTCCGCCTGCGGCTTCGAGACCTCCTGGATCTCCCAGGGCTGCATGAACATCACCACCAAGGCCCAATTCGAGGACCTCGTCGGCCAAACCGCCCTACACGACGTCGTTTCCAACTGCAACCAGTCGCTCGAGAACAACTCCCCCTGCGCCGCCTGCACCACCAGCCTCTCCAGCCTCTCCGCCACCTATTTGACCGGAGAATCCATCGGCAACGTCTCCGGCTGCACCGCCTACCCGTCCATTTACGCCGCCGCGTTCGCCAATCCGTACGGCCCGACCGATCACGGCAACGCCAAGTGCTTCTTTCAGCTCGATTTCACCACGGCCAGCTCCGGTAAGAAGAAAAGGACTCTGATTTTGATTGTGTTGGTTGCCTGTGGTTTCGGGTTGTTGGTTTTAATCGGTGGGATTTGGTTCTCATGGCACAAGTACGAAGACTATATATTCAACAAGAGGCGACAGGATGCTAATGATAGAATTGAGTCGGGTTTGGGTAATGCATTAGAGTCCATTAGTGGGAGTACTACTTTGGTTAAGTTCACATTCGAAGAGATTAGCAAGGCTACTAAGAATTTCTCTAGAGATAATATAATCGGGAGGGGAGGATATGGGAATGTGTACAAGGGCACTCTGGCTGATGGCTCTGAAGTTGCATTGAAGAGGTTCAAGAATTGCTCTGCTGCCGGTGACGCGAATTTCGCTCACGAGGTTGAGGTGATTGCGAGCGTTAGGCATGTGAATCTTGTTGCTTTGAGAGGTTATTGTACCGCGACGACGCATTTGGTAGGTCACCAGAGGATCATTGTGTGTGATTTGATGAAGAATGGGAGTCTCCATGACCATTTGTTTGGGACTTTTGAGAACAAGCTCAGTTGGCCAGTTCGGCAAAGGATTGCGCTAGGGACTGCGAGGGGGTTGGCTTATTTGCATTACGGGGCTCAACCGTCCATCATTCATAGGGATATTAAAGCAAATAACATACTTTTGGATGAGGATTTTGAGGCCAAAGTGGCGGATTTTGGACTTGCAAAGTTTACGCCCGAGGGAATGACACATTTGAGCACTAGGGTGGCTGGAACGATGGGTTATCTTGCTCCCGAGTATGCATTATATGGGCAGCTGACGGAGAAAAGTGATGTTTATAGCTTTGGTGTTGTGATGCTTGAGCTTTTGAGTGGAAAGAAAGCACTCCATGTGAACAATGATAGTCAGCCGTCACTTGTGACGGATTGGGCATGGTCCATGGTGCGAGAAGGAAAAGCGCTGGATGTCATTGAAGATATGCCAGAGAAGGGTTCACCTGAAGTTTTAGAGAAGTATGTATTGGTTGCCGTTCTTTGTTCTCATCCACAGTTGTATGCTAGGCCAACAATGGATCAGGTTGTAAAAATGTTGGAAACAGACTTGTCAGTTTCCTCAATCCCGGAAAGACCAATTCCCCTTGTGGCTGAGATTGATGATATAGAAAGATACAGTAGCAGTGGCTCAATTCAGCTCTCTAGTACAGGAGGCTTCCAGACTTACACATTTGAAAGTAGCCTTCCTTGCGACCATAAGGGAGACGGAGAAAGTTCAGGTTCAACAACAGAAGGGAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

638

Amino Acids

69.76

Weight (kDa)

5.76

Isoelectric Point (pI)

45.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SPARK	PF19160	29 - 181	3.7e-37	SPARK
Pkinase	PF00069	299 - 569	1.3e-42	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	301 - 571	9.6e-42	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001738)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G48010
fragaria_vesca	FvH4_2g24291 FvH4_2g24291 FvH4_6g05000 FvH4_6g05020
malus_domestica	MD03G1272600.v1.1 MD04G1211500.v1.1 MD11G1293500.v1.1 MD12G1226500.v1.1
prunus_persica	Prupe.6G332100_v2.0.a1 Prupe.6G332200_v2.0.a1 Prupe.8G248800_v2.0.a1
pyrus_communis	pycom03g21710 pycom11g25970
rosa_chinensis	RchiOBHm_Chr3g0453511 RchiOBHm_Chr3g0453521 RchiOBHm_Chr5g0002211 RchiOBHm_Chr6g0292151
rosa_laevigata	RLG00000012057 RLG00000025506 RLG00000025507 RLG00000031008
rosa_multiflora	Rmu_co8460761.1_g000001 Rmu_sc0000510.1_g000020
rosa_roxburghii	Rroxscaffold_1G00073750 Rroxscaffold_2G00125960 Rroxscaffold_6G00430490 Rroxscaffold_7G00175400
rosa_rugosa	Rorug02G0649700 Rorug02G0649800 Rorug06G0223800
rosa_samantha	Rh1BG359400 Rh3AG052600 Rh3AG052700 Rh3BG054100 Rh3BG054200 Rh3CG053400 Rh3CG053500 Rh3DG054200 Rh3DG054300 Rh6AG337200 Rh6BG344400 Rh6CG351200 Rh6DG337400 Rh7BG191800
rosa_wichuraiana	Rw3G004050 Rw3G004060 Rw6G029350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	296
AatII	GACGTC	1 cut(s)	423
AccBSI	CCGCTC	1 cut(s)	100
AccII	CGCG	3 cut(s)	572, 1010, 1088
AclWI	GGATC	4 cut(s)	106, 353, 1124, 1701
AcoI	YGGCCR	3 cut(s)	400, 642, 1193
AcsI	RAATTY	3 cut(s)	293, 889, 1012
AcuI	CTGAAG	2 cut(s)	984, 1641
AcyI	GRCGYC	1 cut(s)	420
AfaI	GTAC	7 cut(s)	176, 587, 749, 841, 938, 1084, 1822
AfeI	AGCGCT	1 cut(s)	1581
AfiI	CCNNNNNNNGG	3 cut(s)	388, 834, 1610
AgsI	TTSAA	6 cut(s)	766, 976, 985, 1595, 1850, 1899
AjnI	CCWGG	2 cut(s)	341, 351
AjuI	GAANNNNNNNTTGG	4 cut(s)	368, 400, 1625, 1657
AleI	CACNNNNGTG	1 cut(s)	1385
AluBI	AGCT	8 cut(s)	220, 628, 648, 1186, 1438, 1464, 1486, 1813
AluI	AGCT	8 cut(s)	220, 628, 648, 1186, 1438, 1464, 1486, 1813
Alw21I	GWGCWC	1 cut(s)	1382
Alw26I	GTCTC	4 cut(s)	329, 538, 1154, 1872
AlwI	GGATC	4 cut(s)	106, 353, 1124, 1701
Ama87I	CYCGRG	4 cut(s)	60, 446, 1358, 1415
Aor51HI	AGCGCT	1 cut(s)	1581
AoxI	GGCC	8 cut(s)	187, 378, 400, 589, 642, 1193, 1320, 1682
ApeKI	GCWGC	5 cut(s)	118, 357, 540, 998, 1435
ApoI	RAATTY	3 cut(s)	293, 889, 1012
ArsI	GACNNNNNNTTYG	8 cut(s)	658, 690, 998, 1030, 1081, 1113, 1148, 1180
Asp700I	GAANNNNTTC	1 cut(s)	980
AspLEI	GCGC	4 cut(s)	144, 467, 1216, 1582
AspS9I	GGNCC	5 cut(s)	267, 379, 391, 590, 1557
AsuC2I	CCSGG	2 cut(s)	132, 1742
AsuHPI	GGTGA	8 cut(s)	76, 214, 361, 628, 1016, 1045, 1100, 1608
AsuII	TTCGAA	1 cut(s)	864
AvaI	CYCGRG	4 cut(s)	60, 446, 1358, 1415
AvaII	GGWCC	3 cut(s)	267, 391, 1557
BaeGI	GKGCMC	2 cut(s)	98, 947
BalI	TGGCCA	1 cut(s)	1195
BanII	GRGCYC	1 cut(s)	1258
BauI	CACGAG	1 cut(s)	1022
BbsI	GAAGAC	1 cut(s)	759
Bbv12I	GWGCWC	1 cut(s)	1382
BbvI	GCAGC	5 cut(s)	130, 344, 527, 985, 1447
BccI	CCATC	4 cut(s)	530, 950, 1274, 1393
BceAI	ACGGC	5 cut(s)	604, 619, 657, 1516, 1634
BciT130I	CCWGG	2 cut(s)	343, 353
BcnI	CCSGG	2 cut(s)	132, 1742
BcoDI	GTCTC	4 cut(s)	329, 538, 1154, 1872
BfaI	CTAG	5 cut(s)	897, 1217, 1383, 1680, 1818
BfmI	CTRYAG	1 cut(s)	116
BfoI	RGCGCY	2 cut(s)	145, 1583
BglI	GCCNNNNNGGC	2 cut(s)	1201, 1328
BisI	GCNGC	9 cut(s)	119, 328, 358, 468, 541, 567, 570, 999, 1436
BlsI	GCNGC	9 cut(s)	120, 329, 359, 469, 542, 568, 571, 1000, 1437
BmcAI	AGTACT	1 cut(s)	841
Bme1390I	CCNGG	4 cut(s)	132, 343, 353, 1742
Bme18I	GGWCC	3 cut(s)	267, 391, 1557
BmeT110I	CYCGRG	4 cut(s)	60, 446, 1358, 1415
BmgT120I	GGNCC	5 cut(s)	267, 379, 391, 590, 1557
BmiI	GGNNCC	1 cut(s)	136
BmrFI	CCNGG	4 cut(s)	132, 343, 353, 1742
BmsI	GCATC	2 cut(s)	772, 1467
BpiI	GAAGAC	1 cut(s)	759
BplI	GAGNNNNNCTC	2 cut(s)	440, 472
BpmI	CTGGAG	2 cut(s)	200, 472
Bpu14I	TTCGAA	1 cut(s)	864
BpuEI	CTTGAG	1 cut(s)	1502
BpuMI	CCSGG	2 cut(s)	132, 1742
BsaHI	GRCGYC	1 cut(s)	420
BsaI	GGTCTC	1 cut(s)	329
BsaJI	CCNNGG	8 cut(s)	130, 184, 351, 352, 375, 639, 1359, 1560
BsaWI	WCCGGW	2 cut(s)	512, 650
BsaXI	ACNNNNNCTCC	6 cut(s)	119, 149, 158, 188, 302, 332
Bsc4I	CCNNNNNNNGG	3 cut(s)	388, 834, 1610
Bse118I	RCCGGY	3 cut(s)	224, 302, 1001
Bse1I	ACTGG	3 cut(s)	172, 439, 1196
BseBI	CCWGG	2 cut(s)	343, 353
BseDI	CCNNGG	8 cut(s)	130, 184, 351, 352, 375, 639, 1359, 1560
BseGI	GGATG	6 cut(s)	5, 177, 787, 1312, 1591, 1663
BseLI	CCNNNNNNNGG	3 cut(s)	388, 834, 1610
BseMII	CTCAG	2 cut(s)	1201, 1758
BseNI	ACTGG	3 cut(s)	172, 439, 1196
BseSI	GKGCMC	2 cut(s)	98, 947
BseXI	GCAGC	5 cut(s)	130, 344, 527, 985, 1447
BseYI	CCCAGC	1 cut(s)	72
BsgI	GTGCAG	2 cut(s)	457, 526
Bsh1236I	CGCG	3 cut(s)	572, 1010, 1088
Bsh1285I	CGRYCG	1 cut(s)	597
BshFI	GGCC	8 cut(s)	189, 380, 402, 591, 644, 1195, 1322, 1684
BsiEI	CGRYCG	1 cut(s)	597
BsiHKAI	GWGCWC	1 cut(s)	1382
BsiHKCI	CYCGRG	4 cut(s)	60, 446, 1358, 1415
BsiSI	CCGG	8 cut(s)	132, 225, 303, 513, 537, 651, 1002, 1742
BsiWI	CGTACG	1 cut(s)	585
BslFI	GGGAC	2 cut(s)	1183, 1234
BslI	CCNNNNNNNGG	3 cut(s)	388, 834, 1610
BsmAI	GTCTC	4 cut(s)	329, 538, 1154, 1872
BsmBI	CGTCTC	2 cut(s)	538, 1872
BsmFI	GGGAC	2 cut(s)	1183, 1234
BsnI	GGCC	8 cut(s)	189, 380, 402, 591, 644, 1195, 1322, 1684
Bso31I	GGTCTC	1 cut(s)	329
BsoBI	CYCGRG	4 cut(s)	60, 446, 1358, 1415
Bsp119I	TTCGAA	1 cut(s)	864
Bsp1286I	GDGCHC	4 cut(s)	98, 947, 1258, 1382
Bsp1407I	TGTACA	1 cut(s)	936
Bsp143I	GATC	5 cut(s)	111, 345, 598, 1116, 1693
Bsp19I	CCATGG	1 cut(s)	1560
BspANI	GGCC	8 cut(s)	189, 380, 402, 591, 644, 1195, 1322, 1684
BspCNI	CTCAG	2 cut(s)	1200, 1759
BspFNI	CGCG	3 cut(s)	572, 1010, 1088
BspLI	GGNNCC	1 cut(s)	136
BspMAI	CTGCAG	1 cut(s)	120
BspPI	GGATC	4 cut(s)	106, 353, 1124, 1701
BspT104I	TTCGAA	1 cut(s)	864
BspTNI	GGTCTC	1 cut(s)	329
BsrBI	CCGCTC	1 cut(s)	100
BsrFI	RCCGGY	3 cut(s)	224, 302, 1001
BsrGI	TGTACA	1 cut(s)	936
BsrI	ACTGG	3 cut(s)	172, 439, 1196
BssAI	RCCGGY	3 cut(s)	224, 302, 1001
BssECI	CCNNGG	8 cut(s)	130, 184, 351, 352, 375, 639, 1359, 1560
BssMI	GATC	5 cut(s)	111, 345, 598, 1116, 1693
BssNI	GRCGYC	1 cut(s)	420
BssSI	CACGAG	1 cut(s)	1022
BssT1I	CCWWGG	3 cut(s)	184, 375, 1560
Bst2BI	CACGAG	1 cut(s)	1022
Bst2UI	CCWGG	2 cut(s)	343, 353
Bst4CI	ACNGT	4 cut(s)	88, 1263, 1671, 1793
Bst6I	CTCTTC	2 cut(s)	861, 971
BstACI	GRCGYC	1 cut(s)	420
BstAUI	TGTACA	1 cut(s)	936
BstBI	TTCGAA	1 cut(s)	864
BstC8I	GCNNGC	6 cut(s)	98, 304, 325, 472, 646, 1042
BstDEI	CTNAG	3 cut(s)	885, 1187, 1767
BstDSI	CCRYGG	2 cut(s)	639, 1560
BstEII	GGTNACC	1 cut(s)	1106
BstF5I	GGATG	6 cut(s)	5, 177, 787, 1312, 1591, 1663
BstFNI	CGCG	3 cut(s)	572, 1010, 1088
BstH2I	RGCGCY	2 cut(s)	145, 1583
BstHHI	GCGC	4 cut(s)	144, 467, 1216, 1582
BstKTI	GATC	5 cut(s)	114, 348, 601, 1119, 1696
BstMAI	GTCTC	4 cut(s)	329, 538, 1154, 1872
BstMBI	GATC	5 cut(s)	111, 345, 598, 1116, 1693
BstMCI	CGRYCG	1 cut(s)	597
BstNI	CCWGG	2 cut(s)	343, 353
BstNSI	RCATGY	1 cut(s)	1055
BstPI	GGTNACC	1 cut(s)	1106
BstSCI	CCNGG	4 cut(s)	130, 341, 351, 1740
BstSFI	CTRYAG	1 cut(s)	116
BstSLI	GKGCMC	2 cut(s)	98, 947
BstUI	CGCG	3 cut(s)	572, 1010, 1088
BstV1I	GCAGC	5 cut(s)	130, 344, 527, 985, 1447
BstV2I	GAAGAC	1 cut(s)	759
BstX2I	RGATCY	1 cut(s)	345
BstXI	CCANNNNNNTGG	1 cut(s)	1166
BstYI	RGATCY	1 cut(s)	345
BsuRI	GGCC	8 cut(s)	189, 380, 402, 591, 644, 1195, 1322, 1684
BtgI	CCRYGG	2 cut(s)	639, 1560
BtsCI	GGATG	6 cut(s)	5, 177, 787, 1312, 1591, 1663
BtsI	GCAGTG	1 cut(s)	1804
BtsIMutI	CAGTG	1 cut(s)	1804
Cac8I	GCNNGC	6 cut(s)	98, 304, 325, 472, 646, 1042
CfoI	GCGC	4 cut(s)	144, 467, 1216, 1582
Cfr10I	RCCGGY	3 cut(s)	224, 302, 1001
Cfr13I	GGNCC	5 cut(s)	267, 379, 391, 590, 1557
CseI	GACGC	2 cut(s)	1016, 1102
Csp6I	GTAC	7 cut(s)	175, 586, 748, 840, 937, 1083, 1821
CviAII	CATG	8 cut(s)	163, 361, 738, 1052, 1154, 1511, 1554, 1561
CviQI	GTAC	7 cut(s)	175, 586, 748, 840, 937, 1083, 1821
DdeI	CTNAG	3 cut(s)	885, 1187, 1767
DpnI	GATC	5 cut(s)	113, 347, 600, 1118, 1695
DpnII	GATC	5 cut(s)	111, 345, 598, 1116, 1693
DrdI	GACNNNNNNGTC	1 cut(s)	296
DseDI	GACNNNNNNGTC	1 cut(s)	296
EaeI	YGGCCR	3 cut(s)	400, 642, 1193
Eam1104I	CTCTTC	2 cut(s)	861, 971
EarI	CTCTTC	2 cut(s)	861, 971
EciI	GGCGGA	5 cut(s)	182, 259, 310, 487, 1346
Eco130I	CCWWGG	3 cut(s)	184, 375, 1560
Eco147I	AGGCCT	1 cut(s)	189
Eco24I	GRGCYC	1 cut(s)	1258
Eco31I	GGTCTC	1 cut(s)	329
Eco47I	GGWCC	3 cut(s)	267, 391, 1557
Eco47III	AGCGCT	1 cut(s)	1581
Eco57I	CTGAAG	2 cut(s)	984, 1641
Eco88I	CYCGRG	4 cut(s)	60, 446, 1358, 1415
Eco91I	GGTNACC	1 cut(s)	1106
EcoO109I	RGGNCCY	1 cut(s)	391
EcoO65I	GGTNACC	1 cut(s)	1106
EcoRII	CCWGG	2 cut(s)	341, 351
EcoT14I	CCWWGG	3 cut(s)	184, 375, 1560
EcoT22I	ATGCAT	2 cut(s)	820, 1426
EcoT38I	GRGCYC	1 cut(s)	1258
ErhI	CCWWGG	3 cut(s)	184, 375, 1560
Esp3I	CGTCTC	2 cut(s)	538, 1872
FaeI	CATG	8 cut(s)	166, 364, 741, 1055, 1157, 1514, 1557, 1564
FalI	AAGNNNNNCTT	2 cut(s)	605, 637
FaqI	GGGAC	2 cut(s)	1183, 1234
FatI	CATG	8 cut(s)	162, 360, 737, 1051, 1153, 1510, 1553, 1560
FauI	CCCGC	3 cut(s)	17, 105, 249
Fnu4HI	GCNGC	9 cut(s)	119, 328, 358, 468, 541, 567, 570, 999, 1436
FokI	GGATG	5 cut(s)	164, 794, 1319, 1598, 1650
FriOI	GRGCYC	1 cut(s)	1258
Fsp4HI	GCNGC	9 cut(s)	119, 328, 358, 468, 541, 567, 570, 999, 1436
FspBI	CTAG	5 cut(s)	897, 1217, 1383, 1680, 1818
GlaI	GCGC	4 cut(s)	143, 466, 1215, 1581
GluI	GCNGC	9 cut(s)	119, 328, 358, 468, 541, 567, 570, 999, 1436
GsaI	CCCAGC	1 cut(s)	76
GsuI	CTGGAG	2 cut(s)	200, 472
HaeII	RGCGCY	2 cut(s)	145, 1583
HaeIII	GGCC	8 cut(s)	189, 380, 402, 591, 644, 1195, 1322, 1684
HapII	CCGG	8 cut(s)	132, 225, 303, 513, 537, 651, 1002, 1742
HgaI	GACGC	2 cut(s)	1016, 1102
HhaI	GCGC	4 cut(s)	144, 467, 1216, 1582
Hin1I	GRCGYC	1 cut(s)	420
Hin1II	CATG	8 cut(s)	166, 364, 741, 1055, 1157, 1514, 1557, 1564
Hin6I	GCGC	4 cut(s)	142, 465, 1214, 1580
HinP1I	GCGC	4 cut(s)	142, 465, 1214, 1580
HincII	GTYRAC	1 cut(s)	57
HindII	GTYRAC	1 cut(s)	57
HinfI	GANTC	8 cut(s)	203, 257, 518, 667, 800, 824, 1057, 1147
HpaII	CCGG	8 cut(s)	132, 225, 303, 513, 537, 651, 1002, 1742
HphI	GGTGA	8 cut(s)	76, 214, 361, 628, 1016, 1045, 1100, 1608
Hpy166II	GTNNAC	6 cut(s)	57, 858, 937, 1353, 1516, 1616
Hpy188I	TCNGA	3 cut(s)	256, 672, 964
Hpy188III	TCNNGA	9 cut(s)	109, 334, 448, 897, 913, 985, 1022, 1415, 1834
Hpy8I	GTNNAC	6 cut(s)	57, 858, 937, 1353, 1516, 1616
Hpy99I	CGWCG	7 cut(s)	203, 302, 401, 422, 425, 1093, 1096
HpyAV	CCTTC	4 cut(s)	1565, 1603, 1868, 1901
HpyCH4III	ACNGT	4 cut(s)	88, 1263, 1671, 1793
HpyCH4IV	ACGT	3 cut(s)	81, 420, 531
HpyF3I	CTNAG	3 cut(s)	885, 1187, 1767
HpySE526I	ACGT	3 cut(s)	81, 420, 531
Hsp92I	GRCGYC	1 cut(s)	420
Hsp92II	CATG	8 cut(s)	166, 364, 741, 1055, 1157, 1514, 1557, 1564
HspAI	GCGC	4 cut(s)	142, 465, 1214, 1580
KroI	GCCGGC	1 cut(s)	302
KroNI	GCCGGC	1 cut(s)	304
Kzo9I	GATC	5 cut(s)	111, 345, 598, 1116, 1693
LmnI	GCTCC	4 cut(s)	140, 323, 653, 1417
Lsp1109I	GCAGC	5 cut(s)	130, 344, 527, 985, 1447
LweI	GCATC	2 cut(s)	772, 1467
MaeI	CTAG	5 cut(s)	897, 1217, 1383, 1680, 1818
MaeII	ACGT	3 cut(s)	81, 420, 531
MaeIII	GTNAC	5 cut(s)	82, 1004, 1106, 1533, 1540
MalI	GATC	5 cut(s)	113, 347, 600, 1118, 1695
MbiI	CCGCTC	1 cut(s)	100
MboI	GATC	5 cut(s)	111, 345, 598, 1116, 1693
MboII	GAAGA	6 cut(s)	670, 764, 878, 988, 1150, 1607
MflI	RGATCY	1 cut(s)	345
MhlI	GDGCHC	4 cut(s)	98, 947, 1258, 1382
MlsI	TGGCCA	1 cut(s)	1195
MluCI	AATT	8 cut(s)	293, 383, 795, 889, 988, 1012, 1752, 1806
MluNI	TGGCCA	1 cut(s)	1195
MlyI	GAGTC	5 cut(s)	251, 661, 809, 833, 1156
MmeI	TCCRAC	3 cut(s)	279, 453, 1692
Mox20I	TGGCCA	1 cut(s)	1195
Mph1103I	ATGCAT	2 cut(s)	820, 1426
MroNI	GCCGGC	1 cut(s)	302
MroXI	GAANNNNTTC	1 cut(s)	980
MscI	TGGCCA	1 cut(s)	1195
MseI	TTAA	3 cut(s)	716, 852, 1284
MslI	CAYNNNNRTG	1 cut(s)	1385
Msp20I	TGGCCA	1 cut(s)	1195
MspA1I	CMGCKG	1 cut(s)	1438
MspI	CCGG	8 cut(s)	132, 225, 303, 513, 537, 651, 1002, 1742
MspR9I	CCNGG	4 cut(s)	132, 343, 353, 1742
MvaI	CCWGG	2 cut(s)	343, 353
MvnI	CGCG	3 cut(s)	572, 1010, 1088
NaeI	GCCGGC	1 cut(s)	304
NciI	CCSGG	2 cut(s)	132, 1742
NcoI	CCATGG	1 cut(s)	1560
NdeII	GATC	5 cut(s)	111, 345, 598, 1116, 1693
NgoMIV	GCCGGC	1 cut(s)	302
NlaIII	CATG	8 cut(s)	166, 364, 741, 1055, 1157, 1514, 1557, 1564
NlaIV	GGNNCC	1 cut(s)	136
NmuCI	GTSAC	5 cut(s)	82, 1004, 1106, 1533, 1540
NsiI	ATGCAT	2 cut(s)	820, 1426
NspI	RCATGY	1 cut(s)	1055
NspV	TTCGAA	1 cut(s)	864
OliI	CACNNNNGTG	1 cut(s)	1385
PaeR7I	CTCGAG	2 cut(s)	60, 446
PasI	CCCWGGG	1 cut(s)	352
PceI	AGGCCT	1 cut(s)	189
PcsI	WCGNNNNNNNCGW	3 cut(s)	306, 393, 594
PdiI	GCCGGC	1 cut(s)	304
PdmI	GAANNNNTTC	1 cut(s)	980
PfeI	GAWTC	3 cut(s)	203, 518, 1057
Pfl23II	CGTACG	1 cut(s)	585
PflFI	GACNNNGTC	1 cut(s)	395
PfoI	TCCNGGA	2 cut(s)	341, 1740
PkrI	GCNGC	9 cut(s)	120, 329, 359, 469, 542, 568, 571, 1000, 1437
PleI	GAGTC	5 cut(s)	251, 661, 808, 832, 1155
PpsI	GAGTC	5 cut(s)	251, 661, 808, 832, 1155
PpuMI	RGGWCCY	1 cut(s)	391
Psp5II	RGGWCCY	1 cut(s)	391
Psp6I	CCWGG	2 cut(s)	341, 351
PspEI	GGTNACC	1 cut(s)	1106
PspFI	CCCAGC	1 cut(s)	72
PspGI	CCWGG	2 cut(s)	341, 351
PspLI	CGTACG	1 cut(s)	585
PspN4I	GGNNCC	1 cut(s)	136
PspPI	GGNCC	5 cut(s)	267, 379, 391, 590, 1557
PspPPI	RGGWCCY	1 cut(s)	391
PspXI	VCTCGAGB	1 cut(s)	60
PstI	CTGCAG	1 cut(s)	120
PsuI	RGATCY	1 cut(s)	345
PsyI	GACNNNGTC	1 cut(s)	395
PvuII	CAGCTG	1 cut(s)	1438
RsaI	GTAC	7 cut(s)	176, 587, 749, 841, 938, 1084, 1822
RsaNI	GTAC	7 cut(s)	175, 586, 748, 840, 937, 1083, 1821
RseI	CAYNNNNRTG	1 cut(s)	1385
SaqAI	TTAA	3 cut(s)	716, 852, 1284
SatI	GCNGC	9 cut(s)	119, 328, 358, 468, 541, 567, 570, 999, 1436
Sau3AI	GATC	5 cut(s)	111, 345, 598, 1116, 1693
Sau96I	GGNCC	5 cut(s)	267, 379, 391, 590, 1557
ScaI	AGTACT	1 cut(s)	841
SchI	GAGTC	5 cut(s)	251, 661, 809, 833, 1156
ScrFI	CCNGG	4 cut(s)	132, 343, 353, 1742
SduI	GDGCHC	4 cut(s)	98, 947, 1258, 1382
SfaNI	GCATC	2 cut(s)	772, 1467
SfcI	CTRYAG	1 cut(s)	116
Sfr274I	CTCGAG	2 cut(s)	60, 446
SfuI	TTCGAA	1 cut(s)	864
SinI	GGWCC	3 cut(s)	267, 391, 1557
SlaI	CTCGAG	2 cut(s)	60, 446
SmiMI	CAYNNNNRTG	1 cut(s)	1385
SmlI	CTYRAG	3 cut(s)	60, 446, 1481
SmoI	CTYRAG	3 cut(s)	60, 446, 1481
Sse9I	AATT	8 cut(s)	293, 383, 795, 889, 988, 1012, 1752, 1806
SseBI	AGGCCT	1 cut(s)	189
SspMI	CTAG	5 cut(s)	897, 1217, 1383, 1680, 1818
StuI	AGGCCT	1 cut(s)	189
StyD4I	CCNGG	4 cut(s)	130, 341, 351, 1740
StyI	CCWWGG	3 cut(s)	184, 375, 1560
TaaI	ACNGT	4 cut(s)	88, 1263, 1671, 1793
TaiI	ACGT	3 cut(s)	84, 423, 534
TaqI	TCGA	9 cut(s)	61, 126, 201, 309, 333, 387, 447, 630, 864
TaqII	GACCGA	1 cut(s)	611
TasI	AATT	8 cut(s)	293, 383, 795, 889, 988, 1012, 1752, 1806
TatI	WGTACW	4 cut(s)	174, 839, 936, 1820
TauI	GCSGC	4 cut(s)	330, 470, 569, 572
TfiI	GAWTC	3 cut(s)	203, 518, 1057
Tru1I	TTAA	3 cut(s)	716, 852, 1284
Tru9I	TTAA	3 cut(s)	716, 852, 1284
TscAI	CASTG	1 cut(s)	1804
TseFI	GTSAC	5 cut(s)	82, 1004, 1106, 1533, 1540
TseI	GCWGC	5 cut(s)	118, 357, 540, 998, 1435
Tsp45I	GTSAC	5 cut(s)	82, 1004, 1106, 1533, 1540
TspDTI	ATGAA	4 cut(s)	167, 377, 1151, 1262
TspGWI	ACGGA	5 cut(s)	81, 573, 1457, 1559, 1896
TspRI	CASTG	1 cut(s)	1804
Tth111I	GACNNNGTC	1 cut(s)	395
VpaK11BI	GGWCC	3 cut(s)	267, 391, 1557
XapI	RAATTY	3 cut(s)	293, 889, 1012
XbaI	TCTAGA	1 cut(s)	896
XceI	RCATGY	1 cut(s)	1055
XhoI	CTCGAG	2 cut(s)	60, 446
XmnI	GAANNNNTTC	1 cut(s)	980
XspI	CTAG	5 cut(s)	897, 1217, 1383, 1680, 1818
ZraI	GACGTC	1 cut(s)	421
ZrmI	AGTACT	1 cut(s)	841
Zsp2I	ATGCAT	2 cut(s)	820, 1426

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.