Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0001738

Physical Location & Seq

Genomic Coordinates Reverse (-)

46272391 .. 46274549

Gene Structure 2159 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000025507

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1857 bp

ATGCTGCTCTTCTTCTTCTACCTCTATCTTGGATTAGTACCAATTAGCTTTCCTAGTTGTGGCTTCTTCCCTGTTAGTGGCCAAGAAACACCACGTACAGAGTTGGGTTTCTTTTCATGTCCATTGAACATTGAGGCTCTCAAAGAATTAATCTTCGAAGGTGCTCCGAAAGTTCTTGATGTCCAGACTGAGTGCCAGTACATCCTCGAAGGAATCCGATTGCTTCGATCGGATTATCTCCGATCCACCGGGTACTTTTCCCCACCTTCCTCTACCATAATCCCATGCTGGGATGCATACCAAGCTTTGATCAGCGAATCGATACCGGATTTAAAAATTGGATCCACTTGTGGGTTTAAGCCCACTTTGCTGTCAGAGACTTGTATGAATATCACAACTCGGTCTCAATTTGAAAGGTTGATTTCCAAATCAGAACTGCAGGTAATTGAGGATGCTTGTAGCCAGTCTCTAGATGATTATTCCCCTTGTTTTTCGTGTAGGCGCATTTTGTCGAGTCTGAATATGTCTCATTTTGGTAGAGGGTATGATGGGAATGTAGCAGATTGCAATGGATATCCATATATTTATGCTGCAGCTTTTGGTAATAGGAAGGGACCAATAAATTTGAGTACAGCCAAGTGTTTGTTCTCACTAAACTTCAATTCTTTTGATAGAAAAACTAAAAACAAGCACAGGGTTTTGCTTTGGGCTGTGTTGATAGGAAGTGTGTGTGGATTCGCTGTGGCATTTTTGGTGGTTTGGGTTCTTTGGGGGAGGCATAGGAGAAGGAATAAGAAGATGAAGGATCAAAGTGAGACGACGGGGTTTGTTTCCGCCATAGAAATTATAGGTGAGAATACCAGCTTGGCTAGGTTCCCATTTGAGGAACTTAAAAAGGCCACAGAGAATTTCGCCAGGGAGAACATAATTGGGATTGGAGGCTATGGAAATGTGTACAAGGGTACATTGGCAGATGGGTCTGAAGTAGCTTTGAAAAGGTTCAAGAACTTGTCAGCTGCCGGAGATGTAATGTTTGCACATGAGTTGGAGGTTATTGCTAGCATTCGGCATGTGAATCTTGTCGCGGTGAGAGGCTATTGTACTATAAAAGTGCCTAGGCAAGGCCACCAAAGGATAATGGTGTGTGACTTGGTGAACAATGGAAGTCTCTACGAACATCTTTTCGATTCAAGGATGAAGAAGAAACAGCTCAGCTGGCCAATTCGTCAGAAGATCGCACTTGGAATGGCTCGCGGATTGGCTTATTTGCACTATGGAGTGCAGCCTGCTGTAATCCACAGAGATGTCAAAGCAAGCAATGTGCTTCTAGACGAAGAATTTGAGCCAAAACTGGTTGATTTTGGTTTAGCAAAGTTCACACCAGAAGGACAGACACATATGCACACAAGGGTGGCTGGGACATTTGGGTATGTTGCACCGGAGTATGCTTTGTATGGGCAGTTATCCGAAAGAAGCGATGTCTACAGTTTTGGGGTAGTGCTTCTTGAACTTTTGAGTGGAAAGAGAGCAGTTATTGAAGCTAGTGGTACCACAACTTTGCTTTTGGTAGATTGGGCTTGGTCACAAGTGAGGAAAGGCAGACCATTAGATGTTATAGACGAAAGCATTCAGAACTTAAGCTCTAAGGAAGAAATGGAAAACTATGTAATGGTTGCAGTTCTTTCTGCTCATCCGGAATCACATGCGAGGCCAACAATGGACCAAATTGTCAAGATATTGGAAAGGGATTTTCAAGTTCCCTCAAACATGGACGCCTCTATTCATAAAATCGATCAGTCGGCAAGCTCTAGTGGTTCGAGTTACATTACAAGCAATACTGAGTGGAGTTCTGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

619

Amino Acids

69.13

Weight (kDa)

7.19

Isoelectric Point (pI)

47.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SPARK	PF19160	39 - 189	7.1e-29	SPARK
Pkinase	PF00069	305 - 578	3.5e-39	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	307 - 579	1.1e-37	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001738)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G48010
fragaria_vesca	FvH4_2g24291 FvH4_2g24291 FvH4_6g05000 FvH4_6g05020
malus_domestica	MD03G1272600.v1.1 MD04G1211500.v1.1 MD11G1293500.v1.1 MD12G1226500.v1.1
prunus_persica	Prupe.6G332100_v2.0.a1 Prupe.6G332200_v2.0.a1 Prupe.8G248800_v2.0.a1
pyrus_communis	pycom03g21710 pycom11g25970
rosa_chinensis	RchiOBHm_Chr3g0453511 RchiOBHm_Chr3g0453521 RchiOBHm_Chr5g0002211 RchiOBHm_Chr6g0292151
rosa_laevigata	RLG00000012057 RLG00000025506 RLG00000025507 RLG00000031008
rosa_multiflora	Rmu_co8460761.1_g000001 Rmu_sc0000510.1_g000020
rosa_roxburghii	Rroxscaffold_1G00073750 Rroxscaffold_2G00125960 Rroxscaffold_6G00430490 Rroxscaffold_7G00175400
rosa_rugosa	Rorug02G0649700 Rorug02G0649800 Rorug06G0223800
rosa_samantha	Rh1BG359400 Rh3AG052600 Rh3AG052700 Rh3BG054100 Rh3BG054200 Rh3CG053400 Rh3CG053500 Rh3DG054200 Rh3DG054300 Rh6AG337200 Rh6BG344400 Rh6CG351200 Rh6DG337400 Rh7BG191800
rosa_wichuraiana	Rw3G004050 Rw3G004060 Rw6G029350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1727
Acc36I	ACCTGC	1 cut(s)	430
Acc65I	GGTACC	1 cut(s)	1547
AccB1I	GGYRCC	1 cut(s)	1547
AccI	GTMKAC	1 cut(s)	1482
AccII	CGCG	2 cut(s)	1085, 1254
AccIII	TCCGGA	1 cut(s)	1693
AciI	CCGC	3 cut(s)	834, 1085, 1254
AclWI	GGATC	4 cut(s)	237, 336, 349, 813
AcoI	YGGCCR	2 cut(s)	79, 1217
AcsI	RAATTY	3 cut(s)	622, 907, 1337
AcuI	CTGAAG	1 cut(s)	1002
AcyI	GRCGYC	1 cut(s)	1773
AfaI	GTAC	9 cut(s)	39, 97, 200, 254, 631, 956, 964, 1102, 1549
AfiI	CCNNNNNNNGG	6 cut(s)	59, 77, 289, 351, 883, 1121
AflII	CTTAAG	1 cut(s)	1636
AgsI	TTSAA	9 cut(s)	127, 413, 661, 994, 1003, 1191, 1508, 1538, 1754
AjnI	CCWGG	1 cut(s)	914
AjuI	GAANNNNNNNTTGG	4 cut(s)	629, 661, 848, 880
AleI	CACNNNNGTG	1 cut(s)	1409
Alw21I	GWGCWC	1 cut(s)	166
Alw26I	GTCTC	6 cut(s)	371, 408, 471, 531, 809, 1172
AlwI	GGATC	4 cut(s)	237, 336, 349, 813
Aor13HI	TCCGGA	1 cut(s)	1693
AoxI	GGCC	5 cut(s)	79, 897, 1123, 1217, 1709
ApeKI	GCWGC	5 cut(s)	4, 590, 593, 1016, 1282
ApoI	RAATTY	3 cut(s)	622, 907, 1337
ArsI	GACNNNNNNTTYG	2 cut(s)	1322, 1354
AseI	ATTAAT	1 cut(s)	149
Asp700I	GAANNNNTTC	2 cut(s)	998, 1626
Asp718I	GGTACC	1 cut(s)	1547
AspA2I	CCTAGG	1 cut(s)	1115
AspLEI	GCGC	1 cut(s)	504
AspS9I	GGNCC	2 cut(s)	614, 1720
AsuC2I	CCSGG	1 cut(s)	250
AsuHPI	GGTGA	3 cut(s)	863, 1099, 1165
AsuII	TTCGAA	1 cut(s)	156
AsuNHI	GCTAGC	1 cut(s)	1058
AvaII	GGWCC	2 cut(s)	614, 1720
AvrII	CCTAGG	1 cut(s)	1115
BalI	TGGCCA	2 cut(s)	81, 1219
BamHI	GGATCC	1 cut(s)	341
BanI	GGYRCC	1 cut(s)	1547
Bbv12I	GWGCWC	1 cut(s)	166
BbvI	GCAGC	4 cut(s)	577, 605, 1003, 1294
BccI	CCATC	2 cut(s)	542, 968
BciT130I	CCWGG	1 cut(s)	916
BclI	TGATCA	1 cut(s)	309
BcnI	CCSGG	1 cut(s)	250
BcoDI	GTCTC	6 cut(s)	371, 408, 471, 531, 809, 1172
BfaI	CTAG	8 cut(s)	54, 470, 870, 1059, 1116, 1328, 1542, 1809
BfmI	CTRYAG	3 cut(s)	437, 591, 1483
BfrI	CTTAAG	1 cut(s)	1636
BfuAI	ACCTGC	1 cut(s)	430
BisI	GCNGC	5 cut(s)	5, 591, 594, 1017, 1283
BlnI	CCTAGG	1 cut(s)	1115
BlpI	GCTNAGC	1 cut(s)	1211
BlsI	GCNGC	5 cut(s)	6, 592, 595, 1018, 1284
Bme1390I	CCNGG	2 cut(s)	250, 916
Bme18I	GGWCC	2 cut(s)	614, 1720
BmgT120I	GGNCC	2 cut(s)	614, 1720
BmiI	GGNNCC	4 cut(s)	343, 615, 875, 1549
BmrFI	CCNGG	2 cut(s)	250, 916
BmsI	GCATC	2 cut(s)	283, 442
BmtI	GCTAGC	1 cut(s)	1062
Bpu1102I	GCTNAGC	1 cut(s)	1211
Bpu14I	TTCGAA	1 cut(s)	156
BpuMI	CCSGG	1 cut(s)	250
Bsa29I	ATCGAT	2 cut(s)	320, 1791
BsaAI	YACGTR	1 cut(s)	95
BsaHI	GRCGYC	1 cut(s)	1773
BsaI	GGTCTC	1 cut(s)	408
BsaJI	CCNNGG	2 cut(s)	915, 1115
BsaWI	WCCGGW	3 cut(s)	325, 1438, 1693
Bsc4I	CCNNNNNNNGG	6 cut(s)	59, 77, 289, 351, 883, 1121
Bse1I	ACTGG	3 cut(s)	196, 463, 1356
Bse3DI	GCAATG	2 cut(s)	574, 1324
BseAI	TCCGGA	1 cut(s)	1693
BseBI	CCWGG	1 cut(s)	916
BseCI	ATCGAT	2 cut(s)	320, 1791
BseDI	CCNNGG	2 cut(s)	915, 1115
BseGI	GGATG	5 cut(s)	201, 298, 457, 1200, 1690
BseLI	CCNNNNNNNGG	6 cut(s)	59, 77, 289, 351, 883, 1121
BseMI	GCAATG	2 cut(s)	574, 1324
BseMII	CTCAG	3 cut(s)	180, 1225, 1830
BseNI	ACTGG	3 cut(s)	196, 463, 1356
BseXI	GCAGC	4 cut(s)	577, 605, 1003, 1294
BseYI	CCCAGC	2 cut(s)	288, 1415
BsgI	GTGCAG	1 cut(s)	1301
Bsh1236I	CGCG	2 cut(s)	1085, 1254
Bsh1285I	CGRYCG	1 cut(s)	230
BshFI	GGCC	5 cut(s)	81, 899, 1125, 1219, 1711
BshNI	GGYRCC	1 cut(s)	1547
BshVI	ATCGAT	2 cut(s)	320, 1791
BsiEI	CGRYCG	1 cut(s)	230
BsiHKAI	GWGCWC	1 cut(s)	166
BsiSI	CCGG	5 cut(s)	249, 326, 1020, 1439, 1694
BslFI	GGGAC	2 cut(s)	627, 1432
BslI	CCNNNNNNNGG	6 cut(s)	59, 77, 289, 351, 883, 1121
BsmAI	GTCTC	6 cut(s)	371, 408, 471, 531, 809, 1172
BsmBI	CGTCTC	1 cut(s)	809
BsmFI	GGGAC	2 cut(s)	627, 1432
BsmI	GAATGC	2 cut(s)	1062, 1626
BsnI	GGCC	5 cut(s)	81, 899, 1125, 1219, 1711
Bso31I	GGTCTC	1 cut(s)	408
Bsp119I	TTCGAA	1 cut(s)	156
Bsp1286I	GDGCHC	1 cut(s)	166
Bsp13I	TCCGGA	1 cut(s)	1693
Bsp1407I	TGTACA	1 cut(s)	954
Bsp143I	GATC	7 cut(s)	227, 242, 309, 341, 805, 1233, 1792
Bsp1720I	GCTNAGC	1 cut(s)	1211
BspACI	CCGC	3 cut(s)	834, 1085, 1254
BspANI	GGCC	5 cut(s)	81, 899, 1125, 1219, 1711
BspCNI	CTCAG	3 cut(s)	181, 1224, 1831
BspDI	ATCGAT	2 cut(s)	320, 1791
BspEI	TCCGGA	1 cut(s)	1693
BspFNI	CGCG	2 cut(s)	1085, 1254
BspLI	GGNNCC	4 cut(s)	343, 615, 875, 1549
BspMAI	CTGCAG	2 cut(s)	441, 595
BspMI	ACCTGC	1 cut(s)	430
BspOI	GCTAGC	1 cut(s)	1062
BspPI	GGATC	4 cut(s)	237, 336, 349, 813
BspQI	GCTCTTC	1 cut(s)	14
BspT104I	TTCGAA	1 cut(s)	156
BspT107I	GGYRCC	1 cut(s)	1547
BspTI	CTTAAG	1 cut(s)	1636
BspTNI	GGTCTC	1 cut(s)	408
BsrDI	GCAATG	2 cut(s)	574, 1324
BsrGI	TGTACA	1 cut(s)	954
BsrI	ACTGG	3 cut(s)	196, 463, 1356
BssECI	CCNNGG	2 cut(s)	915, 1115
BssMI	GATC	7 cut(s)	227, 242, 309, 341, 805, 1233, 1792
BssNI	GRCGYC	1 cut(s)	1773
BssT1I	CCWWGG	1 cut(s)	1115
Bst2UI	CCWGG	1 cut(s)	916
Bst4CI	ACNGT	1 cut(s)	1487
Bst6I	CTCTTC	1 cut(s)	14
BstACI	GRCGYC	1 cut(s)	1773
BstAFI	CTTAAG	1 cut(s)	1636
BstAUI	TGTACA	1 cut(s)	954
BstBAI	YACGTR	1 cut(s)	95
BstBI	TTCGAA	1 cut(s)	156
BstC8I	GCNNGC	6 cut(s)	1060, 1217, 1252, 1287, 1315, 1804
BstDEI	CTNAG	4 cut(s)	189, 1211, 1644, 1839
BstENI	CCTNNNNNAGG	1 cut(s)	1119
BstF5I	GGATG	5 cut(s)	201, 298, 457, 1200, 1690
BstFNI	CGCG	2 cut(s)	1085, 1254
BstHHI	GCGC	1 cut(s)	504
BstKTI	GATC	7 cut(s)	230, 245, 312, 344, 808, 1236, 1795
BstMAI	GTCTC	6 cut(s)	371, 408, 471, 531, 809, 1172
BstMBI	GATC	7 cut(s)	227, 242, 309, 341, 805, 1233, 1792
BstMCI	CGRYCG	1 cut(s)	230
BstMWI	GCNNNNNNNGC	3 cut(s)	302, 367, 1216
BstNI	CCWGG	1 cut(s)	916
BstNSI	RCATGY	2 cut(s)	1073, 1706
BstSCI	CCNGG	2 cut(s)	248, 914
BstSFI	CTRYAG	3 cut(s)	437, 591, 1483
BstUI	CGCG	2 cut(s)	1085, 1254
BstV1I	GCAGC	4 cut(s)	577, 605, 1003, 1294
BstX2I	RGATCY	1 cut(s)	341
BstYI	RGATCY	1 cut(s)	341
Bsu15I	ATCGAT	2 cut(s)	320, 1791
BsuRI	GGCC	5 cut(s)	81, 899, 1125, 1219, 1711
BsuTUI	ATCGAT	2 cut(s)	320, 1791
BtgZI	GCGATG	1 cut(s)	1491
BtsCI	GGATG	5 cut(s)	201, 298, 457, 1200, 1690
BveI	ACCTGC	1 cut(s)	430
Cac8I	GCNNGC	6 cut(s)	1060, 1217, 1252, 1287, 1315, 1804
CfoI	GCGC	1 cut(s)	504
Cfr13I	GGNCC	2 cut(s)	614, 1720
ClaI	ATCGAT	2 cut(s)	320, 1791
CseI	GACGC	1 cut(s)	1781
Csp6I	GTAC	9 cut(s)	38, 96, 199, 253, 630, 955, 963, 1101, 1548
CviAII	CATG	6 cut(s)	117, 285, 1040, 1070, 1703, 1768
CviQI	GTAC	9 cut(s)	38, 96, 199, 253, 630, 955, 963, 1101, 1548
DdeI	CTNAG	4 cut(s)	189, 1211, 1644, 1839
DpnI	GATC	7 cut(s)	229, 244, 311, 343, 807, 1235, 1794
DpnII	GATC	7 cut(s)	227, 242, 309, 341, 805, 1233, 1792
DraI	TTTAAA	1 cut(s)	333
DrdI	GACNNNNNNGTC	1 cut(s)	1727
DseDI	GACNNNNNNGTC	1 cut(s)	1727
EaeI	YGGCCR	2 cut(s)	79, 1217
Eam1104I	CTCTTC	1 cut(s)	14
EarI	CTCTTC	1 cut(s)	14
EciI	GGCGGA	1 cut(s)	823
Eco130I	CCWWGG	1 cut(s)	1115
Eco31I	GGTCTC	1 cut(s)	408
Eco32I	GATATC	1 cut(s)	575
Eco47I	GGWCC	2 cut(s)	614, 1720
Eco57I	CTGAAG	1 cut(s)	1002
EcoNI	CCTNNNNNAGG	1 cut(s)	1119
EcoRII	CCWGG	1 cut(s)	914
EcoRV	GATATC	1 cut(s)	575
EcoT14I	CCWWGG	1 cut(s)	1115
EcoT22I	ATGCAT	1 cut(s)	298
ErhI	CCWWGG	1 cut(s)	1115
Esp3I	CGTCTC	1 cut(s)	809
FaeI	CATG	6 cut(s)	120, 288, 1043, 1073, 1706, 1771
FaqI	GGGAC	2 cut(s)	627, 1432
FatI	CATG	6 cut(s)	116, 284, 1039, 1069, 1702, 1767
FauNDI	CATATG	1 cut(s)	1398
FbaI	TGATCA	1 cut(s)	309
FblI	GTMKAC	1 cut(s)	1482
Fnu4HI	GCNGC	5 cut(s)	5, 591, 594, 1017, 1283
FokI	GGATG	5 cut(s)	188, 305, 464, 1207, 1677
Fsp4HI	GCNGC	5 cut(s)	5, 591, 594, 1017, 1283
FspBI	CTAG	8 cut(s)	54, 470, 870, 1059, 1116, 1328, 1542, 1809
GlaI	GCGC	1 cut(s)	503
GluI	GCNGC	5 cut(s)	5, 591, 594, 1017, 1283
GsaI	CCCAGC	2 cut(s)	292, 1419
HaeIII	GGCC	5 cut(s)	81, 899, 1125, 1219, 1711
HapII	CCGG	5 cut(s)	249, 326, 1020, 1439, 1694
HgaI	GACGC	1 cut(s)	1781
HhaI	GCGC	1 cut(s)	504
Hin1I	GRCGYC	1 cut(s)	1773
Hin1II	CATG	6 cut(s)	120, 288, 1043, 1073, 1706, 1771
Hin6I	GCGC	1 cut(s)	502
HinP1I	GCGC	1 cut(s)	502
HindIII	AAGCTT	1 cut(s)	303
HinfI	GANTC	7 cut(s)	213, 317, 514, 735, 1075, 1187, 1697
HpaII	CCGG	5 cut(s)	249, 326, 1020, 1439, 1694
HphI	GGTGA	3 cut(s)	863, 1099, 1165
Hpy166II	GTNNAC	4 cut(s)	955, 1156, 1377, 1483
Hpy188III	TCNNGA	8 cut(s)	176, 184, 470, 1003, 1328, 1505, 1694, 1732
Hpy8I	GTNNAC	4 cut(s)	955, 1156, 1377, 1483
Hpy99I	CGWCG	1 cut(s)	823
HpyAV	CCTTC	7 cut(s)	152, 203, 276, 604, 780, 796, 1379
HpyCH4III	ACNGT	1 cut(s)	1487
HpyCH4IV	ACGT	1 cut(s)	94
HpyF10VI	GCNNNNNNNGC	3 cut(s)	302, 367, 1216
HpyF3I	CTNAG	4 cut(s)	189, 1211, 1644, 1839
HpySE526I	ACGT	1 cut(s)	94
Hsp92I	GRCGYC	1 cut(s)	1773
Hsp92II	CATG	6 cut(s)	120, 288, 1043, 1073, 1706, 1771
HspAI	GCGC	1 cut(s)	502
Kpn2I	TCCGGA	1 cut(s)	1693
KpnI	GGTACC	1 cut(s)	1551
Ksp22I	TGATCA	1 cut(s)	309
Kzo9I	GATC	7 cut(s)	227, 242, 309, 341, 805, 1233, 1792
LguI	GCTCTTC	1 cut(s)	14
LmnI	GCTCC	1 cut(s)	169
Lsp1109I	GCAGC	4 cut(s)	577, 605, 1003, 1294
LweI	GCATC	2 cut(s)	283, 442
MaeI	CTAG	8 cut(s)	54, 470, 870, 1059, 1116, 1328, 1542, 1809
MaeII	ACGT	1 cut(s)	94
MaeIII	GTNAC	3 cut(s)	1145, 1581, 1820
MalI	GATC	7 cut(s)	229, 244, 311, 343, 807, 1235, 1794
MboI	GATC	7 cut(s)	227, 242, 309, 341, 805, 1233, 1792
MflI	RGATCY	1 cut(s)	341
MhlI	GDGCHC	1 cut(s)	166
MlsI	TGGCCA	2 cut(s)	81, 1219
MluNI	TGGCCA	2 cut(s)	81, 1219
MlyI	GAGTC	1 cut(s)	523
MmeI	TCCRAC	1 cut(s)	1026
Mox20I	TGGCCA	2 cut(s)	81, 1219
Mph1103I	ATGCAT	1 cut(s)	298
MroI	TCCGGA	1 cut(s)	1693
MroXI	GAANNNNTTC	2 cut(s)	998, 1626
MscI	TGGCCA	2 cut(s)	81, 1219
MseI	TTAA	5 cut(s)	149, 332, 357, 891, 1637
MslI	CAYNNNNRTG	2 cut(s)	1302, 1409
Msp20I	TGGCCA	2 cut(s)	81, 1219
MspA1I	CMGCKG	2 cut(s)	1016, 1215
MspCI	CTTAAG	1 cut(s)	1636
MspI	CCGG	5 cut(s)	249, 326, 1020, 1439, 1694
MspR9I	CCNGG	2 cut(s)	250, 916
Mva1269I	GAATGC	2 cut(s)	1062, 1626
MvaI	CCWGG	1 cut(s)	916
MvnI	CGCG	2 cut(s)	1085, 1254
MwoI	GCNNNNNNNGC	3 cut(s)	302, 367, 1216
NciI	CCSGG	1 cut(s)	250
NdeI	CATATG	1 cut(s)	1398
NdeII	GATC	7 cut(s)	227, 242, 309, 341, 805, 1233, 1792
NheI	GCTAGC	1 cut(s)	1058
NlaIII	CATG	6 cut(s)	120, 288, 1043, 1073, 1706, 1771
NlaIV	GGNNCC	4 cut(s)	343, 615, 875, 1549
NmuCI	GTSAC	2 cut(s)	1145, 1581
NsiI	ATGCAT	1 cut(s)	298
NspI	RCATGY	2 cut(s)	1073, 1706
NspV	TTCGAA	1 cut(s)	156
OliI	CACNNNNGTG	1 cut(s)	1409
PciSI	GCTCTTC	1 cut(s)	14
PctI	GAATGC	2 cut(s)	1062, 1626
PdmI	GAANNNNTTC	2 cut(s)	998, 1626
PfeI	GAWTC	6 cut(s)	213, 317, 735, 1075, 1187, 1697
PkrI	GCNGC	5 cut(s)	6, 592, 595, 1018, 1284
Ple19I	CGATCG	1 cut(s)	230
PleI	GAGTC	1 cut(s)	522
PpsI	GAGTC	1 cut(s)	522
Ppu21I	YACGTR	1 cut(s)	95
PshBI	ATTAAT	1 cut(s)	149
Psp6I	CCWGG	1 cut(s)	914
PspFI	CCCAGC	2 cut(s)	288, 1415
PspGI	CCWGG	1 cut(s)	914
PspN4I	GGNNCC	4 cut(s)	343, 615, 875, 1549
PspPI	GGNCC	2 cut(s)	614, 1720
PstI	CTGCAG	2 cut(s)	441, 595
PsuI	RGATCY	1 cut(s)	341
PvuI	CGATCG	1 cut(s)	230
PvuII	CAGCTG	2 cut(s)	1016, 1215
RsaI	GTAC	9 cut(s)	39, 97, 200, 254, 631, 956, 964, 1102, 1549
RsaNI	GTAC	9 cut(s)	38, 96, 199, 253, 630, 955, 963, 1101, 1548
RseI	CAYNNNNRTG	2 cut(s)	1302, 1409
SapI	GCTCTTC	1 cut(s)	14
SaqAI	TTAA	5 cut(s)	149, 332, 357, 891, 1637
SatI	GCNGC	5 cut(s)	5, 591, 594, 1017, 1283
Sau3AI	GATC	7 cut(s)	227, 242, 309, 341, 805, 1233, 1792
Sau96I	GGNCC	2 cut(s)	614, 1720
SchI	GAGTC	1 cut(s)	523
ScrFI	CCNGG	2 cut(s)	250, 916
SduI	GDGCHC	1 cut(s)	166
SfaNI	GCATC	2 cut(s)	283, 442
SfcI	CTRYAG	3 cut(s)	437, 591, 1483
SfuI	TTCGAA	1 cut(s)	156
SinI	GGWCC	2 cut(s)	614, 1720
SmiMI	CAYNNNNRTG	2 cut(s)	1302, 1409
SmlI	CTYRAG	1 cut(s)	1636
SmoI	CTYRAG	1 cut(s)	1636
SsiI	CCGC	3 cut(s)	834, 1085, 1254
SspMI	CTAG	8 cut(s)	54, 470, 870, 1059, 1116, 1328, 1542, 1809
StyD4I	CCNGG	2 cut(s)	248, 914
StyI	CCWWGG	1 cut(s)	1115
TaaI	ACNGT	1 cut(s)	1487
TaiI	ACGT	1 cut(s)	97
TaqI	TCGA	8 cut(s)	156, 207, 226, 320, 512, 1185, 1791, 1817
TaqII	GACCGA	1 cut(s)	390
TatI	WGTACW	4 cut(s)	198, 629, 954, 1100
TfiI	GAWTC	6 cut(s)	213, 317, 735, 1075, 1187, 1697
Tru1I	TTAA	5 cut(s)	149, 332, 357, 891, 1637
Tru9I	TTAA	5 cut(s)	149, 332, 357, 891, 1637
TseFI	GTSAC	2 cut(s)	1145, 1581
TseI	GCWGC	5 cut(s)	4, 590, 593, 1016, 1282
Tsp45I	GTSAC	2 cut(s)	1145, 1581
TspDTI	ATGAA	5 cut(s)	105, 401, 815, 1211, 1772
Vha464I	CTTAAG	1 cut(s)	1636
VpaK11BI	GGWCC	2 cut(s)	614, 1720
VspI	ATTAAT	1 cut(s)	149
XagI	CCTNNNNNAGG	1 cut(s)	1119
XapI	RAATTY	3 cut(s)	622, 907, 1337
XbaI	TCTAGA	2 cut(s)	469, 1327
XceI	RCATGY	2 cut(s)	1073, 1706
XmaJI	CCTAGG	1 cut(s)	1115
XmiI	GTMKAC	1 cut(s)	1482
XmnI	GAANNNNTTC	2 cut(s)	998, 1626
XspI	CTAG	8 cut(s)	54, 470, 870, 1059, 1116, 1328, 1542, 1809
Zsp2I	ATGCAT	1 cut(s)	298

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.