Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0001738

Physical Location & Seq

Genomic Coordinates Reverse (-)

41278281 .. 41280194

Gene Structure 1914 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1293500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1914 bp

ATGTCCTCCTCCTTCCTCTTCCTCTTCCTCCTCATCCTCCTCTCCTCCCTCCCCACCCCATCCCTCTGCCAGCTAAACGCCACCGTTCCGTGCCCCCTCAACTTCACCATCCTCCGCCAGTTCGACCCGGGCTCCCGCAACCGCAAACGCTACGACCGCAACACCGAGTGCCAGTACCTCGTCCAGGGCCTCCGCTTCGTCGACTCCGATTACCTCCGCCGCACCGGCAACTTCCTCCCCCCGCCCAACGCCTCCGAGTCCTGCTGGGTCGATTACCAAGCCCTAGCCAACGAGTTCGTCCCCAATTTCGACATTCGCGCCAACTGCGGCTTCCAGACCAGCTGGATCTCCGAAGGATGCACGAACGTCTCCACCCGGTCCCAATTCGAGTCCCAAGTGGGAAACACCTCGCTCAGCGACGTCGCCGCCGCCTGCAGCCAGTCTCTCGAAAACGGCTCGCCCTGCGCCCTCTGCACCACCAGAATGTCCTTGCAGACCTCGCGGTTGACCGGTGAGTCCGTCGGAAACGTCTCCACCTGCACTGCGTACCCGGCCATTTACGTGGCGGCTTTTGTCGGACCGATCGATATGGGCACCGCCGAGTGCTTGTTCTCGCTCAGAAGCCCGGCAAAGAGCTCCGGTAAGAAGAAAAAGACTGTAATTTTGATTGTATTGGTTGTTTGCGGCGTCGGATTGCTGGTTGTGATCGGCGGCGTTTGGTTTTTGTGGCATAAATATGAAGAATTTATGATTAGGAAGAGGAAAAAGGATGCTAGTGATAAAATCGAGCAGGGATTAGGTTCTGCTTTAGATTCAATTAGTGGAAGTACTAATTTAATTAAGTTTAAATACGAGGAGATTAGTGCTGCAACTAAGAATTTCTCTAGGGACAATATAATTGGGAGAGGAGGATATGGGAATGTGTACAAGGGAATTCTGGATGATGGTTCTGAAGTTGCTTTGAAGAGGTTCAAGAACTGCTCGGCTGCTGGCGACGCGAATTTCGCCCACGAGGTTGAGGTGATTGCGAGTGTTAGGCATGTCAATCTTCTTGCTTTGAGAGGTTATTGCATTGCCACTACTCCTTTGGTGGGTCACCAGAGGATCATTGTGTGTGATTTGATGAAGAATGGGAGTCTCCATGACCATTTGTTCGGTTCGTTTGAGAGCAAGCTTAGTTGGCCTATTCGTCAGAGGATCGCGCTGGGGACTGCGAGGGGATTGGCTTATTTGCATTACGGGGCTCAACCAACTATTATCCATAGGGACATCAAAGCTAATAACATACTTTTGGATGAGACATTCGAGGCCAAGGTAGCAGATTTTGGGCTTGCCAAGTTTACACCTGAGGGAATGACGCATTTGAGCACGAGGGTGGCTGGAACAATGGGTTATCTTGCTCCAGAGTATGCGTTGTACGGGCAGTTGACAGATAAAAGTGATGTGTATAGTTTTGGTGTTGTGCTCCTTGAACTTTTGAGTGGAAAGAAAGCGCTCCATGTGATTGATGATAATCAACCGTCACTTGTGACTGATTGGGCGTGGGAGTTGGTGAGGAAAGGAAGACCGCTGGATGTTATTGAAAATGATATGCCGGAGAAAGGTTCACCCGAAGTTCTAGAGAAGTATGTATTGATTGCCGTTCTTTGTTCTCATCCGCAGTTGTATGCTAGGCCAACGATGGATCAGGCTGTTAAAATGTTGGAAACAGATATATCAGTTCCATCAATTCCTGAAAGACCAATTCCCCTTGTGGCAAATATTGACGATATTGAAAGATATAGCAGCAGTGGCTCAATCCAGCTCTCTACTACAGGTGGTTTTCAGACTTTTACATTCGATAGTAACCTACATTCTAACCCTAATAGAGACGGGGAAAGTTCTGGTACCAGAGAGAATGGTGGTGACTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko03010 map03010

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

638

Amino Acids

69.95

Weight (kDa)

6.34

Isoelectric Point (pI)

42.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SPARK	PF19160	27 - 180	6.1e-36	SPARK
Pkinase	PF00069	295 - 566	8.7e-41	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	297 - 564	1.1e-39	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001738)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G48010
fragaria_vesca	FvH4_2g24291 FvH4_2g24291 FvH4_6g05000 FvH4_6g05020
malus_domestica	MD03G1272600.v1.1 MD04G1211500.v1.1 MD11G1293500.v1.1 MD12G1226500.v1.1
prunus_persica	Prupe.6G332100_v2.0.a1 Prupe.6G332200_v2.0.a1 Prupe.8G248800_v2.0.a1
pyrus_communis	pycom03g21710 pycom11g25970
rosa_chinensis	RchiOBHm_Chr3g0453511 RchiOBHm_Chr3g0453521 RchiOBHm_Chr5g0002211 RchiOBHm_Chr6g0292151
rosa_laevigata	RLG00000012057 RLG00000025506 RLG00000025507 RLG00000031008
rosa_multiflora	Rmu_co8460761.1_g000001 Rmu_sc0000510.1_g000020
rosa_roxburghii	Rroxscaffold_1G00073750 Rroxscaffold_2G00125960 Rroxscaffold_6G00430490 Rroxscaffold_7G00175400
rosa_rugosa	Rorug02G0649700 Rorug02G0649800 Rorug06G0223800
rosa_samantha	Rh1BG359400 Rh3AG052600 Rh3AG052700 Rh3BG054100 Rh3BG054200 Rh3CG053400 Rh3CG053500 Rh3DG054200 Rh3DG054300 Rh6AG337200 Rh6BG344400 Rh6CG351200 Rh6DG337400 Rh7BG191800
rosa_wichuraiana	Rw3G004050 Rw3G004060 Rw6G029350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	545
AasI	GACNNNNNNGTC	1 cut(s)	514
AatII	GACGTC	1 cut(s)	423
Acc36I	ACCTGC	1 cut(s)	545
Acc65I	GGTACC	1 cut(s)	1886
AccB1I	GGYRCC	2 cut(s)	593, 1886
AccI	GTMKAC	1 cut(s)	201
AccII	CGCG	4 cut(s)	318, 502, 998, 1202
AclWI	GGATC	4 cut(s)	353, 1112, 1205, 1692
AcoI	YGGCCR	1 cut(s)	552
AcsI	RAATTY	4 cut(s)	743, 877, 933, 1000
AcuI	CTGAAG	1 cut(s)	972
AcyI	GRCGYC	2 cut(s)	420, 687
AdeI	CACNNNGTG	1 cut(s)	168
AfaI	GTAC	6 cut(s)	176, 548, 829, 926, 1418, 1888
AfeI	AGCGCT	1 cut(s)	1494
AfiI	CCNNNNNNNGG	3 cut(s)	184, 1091, 1601
AgeI	ACCGGT	1 cut(s)	509
AgsI	TTSAA	6 cut(s)	816, 964, 973, 1472, 1583, 1775
AjnI	CCWGG	1 cut(s)	183
AjuI	GAANNNNNNNTTGG	2 cut(s)	314, 346
AleI	CACNNNNGTG	1 cut(s)	1373
AluBI	AGCT	6 cut(s)	73, 342, 636, 1174, 1277, 1804
AluI	AGCT	6 cut(s)	73, 342, 636, 1174, 1277, 1804
Alw21I	GWGCWC	3 cut(s)	638, 1370, 1467
Alw26I	GTCTC	6 cut(s)	373, 447, 535, 1142, 1292, 1863
AlwI	GGATC	4 cut(s)	353, 1112, 1205, 1692
Ama87I	CYCGRG	1 cut(s)	127
Aor51HI	AGCGCT	1 cut(s)	1494
AoxI	GGCC	5 cut(s)	187, 552, 1181, 1308, 1673
ApeKI	GCWGC	4 cut(s)	435, 866, 986, 1785
ApoI	RAATTY	4 cut(s)	743, 877, 933, 1000
ArsI	GACNNNNNNTTYG	6 cut(s)	646, 678, 986, 1018, 1136, 1168
AsiGI	ACCGGT	1 cut(s)	509
Asp700I	GAANNNNTTC	1 cut(s)	968
Asp718I	GGTACC	1 cut(s)	1886
AspLEI	GCGC	4 cut(s)	320, 467, 1204, 1495
AspS9I	GGNCC	3 cut(s)	187, 378, 578
AsuC2I	CCSGG	5 cut(s)	128, 129, 376, 551, 626
AsuHPI	GGTGA	6 cut(s)	97, 524, 1033, 1088, 1564, 1599
AvaI	CYCGRG	1 cut(s)	127
AvaII	GGWCC	2 cut(s)	378, 578
AxyI	CCTNAGG	1 cut(s)	1347
BaeGI	GKGCMC	2 cut(s)	95, 596
BanI	GGYRCC	2 cut(s)	593, 1886
BanII	GRGCYC	3 cut(s)	134, 638, 1246
BauI	CACGAG	2 cut(s)	1010, 1369
BbsI	GAAGAC	1 cut(s)	1570
Bbv12I	GWGCWC	3 cut(s)	638, 1370, 1467
BbvI	GCAGC	4 cut(s)	447, 853, 973, 1797
BccI	CCATC	5 cut(s)	67, 116, 938, 1675, 1732
BceAI	ACGGC	2 cut(s)	469, 1625
BciT130I	CCWGG	1 cut(s)	185
BcnI	CCSGG	5 cut(s)	128, 129, 376, 551, 626
BcoDI	GTCTC	6 cut(s)	373, 447, 535, 1142, 1292, 1863
BfaI	CTAG	5 cut(s)	284, 774, 885, 1619, 1671
BfmI	CTRYAG	2 cut(s)	433, 1812
BfoI	RGCGCY	1 cut(s)	1496
BfuAI	ACCTGC	1 cut(s)	545
BglI	GCCNNNNNGGC	1 cut(s)	225
BlpI	GCTNAGC	1 cut(s)	413
BmcAI	AGTACT	1 cut(s)	829
Bme1390I	CCNGG	6 cut(s)	128, 129, 185, 376, 551, 626
Bme18I	GGWCC	2 cut(s)	378, 578
BmeT110I	CYCGRG	1 cut(s)	127
BmgT120I	GGNCC	3 cut(s)	187, 378, 578
BmiI	GGNNCC	4 cut(s)	133, 380, 595, 1888
BmrFI	CCNGG	6 cut(s)	128, 129, 185, 376, 551, 626
BmsI	GCATC	2 cut(s)	347, 760
BpiI	GAAGAC	1 cut(s)	1570
BpmI	CTGGAG	1 cut(s)	1386
Bpu1102I	GCTNAGC	1 cut(s)	413
BpuMI	CCSGG	5 cut(s)	128, 129, 376, 551, 626
Bsa29I	ATCGAT	1 cut(s)	585
BsaAI	YACGTR	1 cut(s)	562
BsaBI	GATNNNNATC	1 cut(s)	1512
BsaHI	GRCGYC	2 cut(s)	420, 687
BsaJI	CCNNGG	3 cut(s)	127, 184, 1311
BsaWI	WCCGGW	2 cut(s)	509, 638
BsaXI	ACNNNNNCTCC	2 cut(s)	116, 146
Bsc4I	CCNNNNNNNGG	3 cut(s)	184, 1091, 1601
Bse118I	RCCGGY	2 cut(s)	224, 509
Bse1I	ACTGG	3 cut(s)	118, 172, 439
Bse21I	CCTNAGG	1 cut(s)	1347
Bse3DI	GCAATG	1 cut(s)	1071
Bse8I	GATNNNNATC	1 cut(s)	1512
BseBI	CCWGG	1 cut(s)	185
BseCI	ATCGAT	1 cut(s)	585
BseDI	CCNNGG	3 cut(s)	127, 184, 1311
BseGI	GGATG	9 cut(s)	33, 59, 108, 362, 775, 946, 1300, 1579, 1654
BseJI	GATNNNNATC	1 cut(s)	1512
BseLI	CCNNNNNNNGG	3 cut(s)	184, 1091, 1601
BseMI	GCAATG	1 cut(s)	1071
BseMII	CTCAG	3 cut(s)	427, 631, 1338
BseNI	ACTGG	3 cut(s)	118, 172, 439
BseRI	GAGGAG	5 cut(s)	20, 29, 34, 869, 921
BseSI	GKGCMC	2 cut(s)	95, 596
BseXI	GCAGC	4 cut(s)	447, 853, 973, 1797
BseYI	CCCAGC	2 cut(s)	264, 1204
BsgI	GTGCAG	2 cut(s)	457, 523
Bsh1236I	CGCG	4 cut(s)	318, 502, 998, 1202
Bsh1285I	CGRYCG	2 cut(s)	157, 585
BshFI	GGCC	5 cut(s)	189, 554, 1183, 1310, 1675
BshNI	GGYRCC	2 cut(s)	593, 1886
BshTI	ACCGGT	1 cut(s)	509
BshVI	ATCGAT	1 cut(s)	585
BsiEI	CGRYCG	2 cut(s)	157, 585
BsiHKAI	GWGCWC	3 cut(s)	638, 1370, 1467
BsiHKCI	CYCGRG	1 cut(s)	127
BsiSI	CCGG	8 cut(s)	128, 225, 376, 510, 551, 626, 639, 1595
BslFI	GGGAC	6 cut(s)	284, 364, 376, 902, 1222, 1280
BslI	CCNNNNNNNGG	3 cut(s)	184, 1091, 1601
BsmAI	GTCTC	6 cut(s)	373, 447, 535, 1142, 1292, 1863
BsmBI	CGTCTC	3 cut(s)	373, 535, 1863
BsmFI	GGGAC	6 cut(s)	284, 364, 376, 902, 1222, 1280
BsnI	GGCC	5 cut(s)	189, 554, 1183, 1310, 1675
BsoBI	CYCGRG	1 cut(s)	127
Bsp1286I	GDGCHC	7 cut(s)	95, 134, 596, 638, 1246, 1370, 1467
Bsp1407I	TGTACA	1 cut(s)	924
Bsp143I	GATC	6 cut(s)	345, 582, 705, 1104, 1197, 1684
Bsp1720I	GCTNAGC	1 cut(s)	413
BspANI	GGCC	5 cut(s)	189, 554, 1183, 1310, 1675
BspCNI	CTCAG	3 cut(s)	426, 630, 1339
BspDI	ATCGAT	1 cut(s)	585
BspFNI	CGCG	4 cut(s)	318, 502, 998, 1202
BspLI	GGNNCC	4 cut(s)	133, 380, 595, 1888
BspMAI	CTGCAG	1 cut(s)	437
BspMI	ACCTGC	1 cut(s)	545
BspPI	GGATC	4 cut(s)	353, 1112, 1205, 1692
BspT107I	GGYRCC	2 cut(s)	593, 1886
BsrDI	GCAATG	1 cut(s)	1071
BsrFI	RCCGGY	2 cut(s)	224, 509
BsrGI	TGTACA	1 cut(s)	924
BsrI	ACTGG	3 cut(s)	118, 172, 439
BssAI	RCCGGY	2 cut(s)	224, 509
BssECI	CCNNGG	3 cut(s)	127, 184, 1311
BssMI	GATC	6 cut(s)	345, 582, 705, 1104, 1197, 1684
BssNI	GRCGYC	2 cut(s)	420, 687
BssSI	CACGAG	2 cut(s)	1010, 1369
BssT1I	CCWWGG	1 cut(s)	1311
Bst2BI	CACGAG	2 cut(s)	1010, 1369
Bst2UI	CCWGG	1 cut(s)	185
Bst4CI	ACNGT	3 cut(s)	85, 658, 1521
Bst6I	CTCTTC	4 cut(s)	23, 29, 752, 959
BstACI	GRCGYC	2 cut(s)	420, 687
BstAUI	TGTACA	1 cut(s)	924
BstBAI	YACGTR	1 cut(s)	562
BstC8I	GCNNGC	6 cut(s)	71, 433, 458, 991, 1172, 1332
BstDEI	CTNAG	5 cut(s)	413, 617, 873, 1175, 1347
BstEII	GGTNACC	1 cut(s)	1094
BstENI	CCTNNNNNAGG	1 cut(s)	182
BstF5I	GGATG	9 cut(s)	33, 59, 108, 362, 775, 946, 1300, 1579, 1654
BstFNI	CGCG	4 cut(s)	318, 502, 998, 1202
BstH2I	RGCGCY	1 cut(s)	1496
BstHHI	GCGC	4 cut(s)	320, 467, 1204, 1495
BstKTI	GATC	6 cut(s)	348, 585, 708, 1107, 1200, 1687
BstMAI	GTCTC	6 cut(s)	373, 447, 535, 1142, 1292, 1863
BstMBI	GATC	6 cut(s)	345, 582, 705, 1104, 1197, 1684
BstMCI	CGRYCG	2 cut(s)	157, 585
BstNI	CCWGG	1 cut(s)	185
BstNSI	RCATGY	1 cut(s)	1043
BstPI	GGTNACC	1 cut(s)	1094
BstSCI	CCNGG	6 cut(s)	126, 127, 183, 374, 549, 624
BstSFI	CTRYAG	2 cut(s)	433, 1812
BstSLI	GKGCMC	2 cut(s)	95, 596
BstUI	CGCG	4 cut(s)	318, 502, 998, 1202
BstV1I	GCAGC	4 cut(s)	447, 853, 973, 1797
BstV2I	GAAGAC	1 cut(s)	1570
BstX2I	RGATCY	1 cut(s)	345
BstXI	CCANNNNNNTGG	1 cut(s)	562
BstYI	RGATCY	1 cut(s)	345
Bsu15I	ATCGAT	1 cut(s)	585
Bsu36I	CCTNAGG	1 cut(s)	1347
BsuRI	GGCC	5 cut(s)	189, 554, 1183, 1310, 1675
BsuTUI	ATCGAT	1 cut(s)	585
BtsCI	GGATG	9 cut(s)	33, 59, 108, 362, 775, 946, 1300, 1579, 1654
BtsI	GCAGTG	2 cut(s)	540, 1795
BtsIMutI	CAGTG	2 cut(s)	540, 1795
BveI	ACCTGC	1 cut(s)	545
Cac8I	GCNNGC	6 cut(s)	71, 433, 458, 991, 1172, 1332
CfoI	GCGC	4 cut(s)	320, 467, 1204, 1495
Cfr10I	RCCGGY	2 cut(s)	224, 509
Cfr13I	GGNCC	3 cut(s)	187, 378, 578
Cfr9I	CCCGGG	1 cut(s)	127
ClaI	ATCGAT	1 cut(s)	585
CpoI	CGGWCCG	1 cut(s)	578
CseI	GACGC	3 cut(s)	676, 1004, 1366
Csp6I	GTAC	6 cut(s)	175, 547, 828, 925, 1417, 1887
CspAI	ACCGGT	1 cut(s)	509
CspI	CGGWCCG	1 cut(s)	578
CviAII	CATG	3 cut(s)	1040, 1142, 1499
CviQI	GTAC	6 cut(s)	175, 547, 828, 925, 1417, 1887
DdeI	CTNAG	5 cut(s)	413, 617, 873, 1175, 1347
DpnI	GATC	6 cut(s)	347, 584, 707, 1106, 1199, 1686
DpnII	GATC	6 cut(s)	345, 582, 705, 1104, 1197, 1684
DraI	TTTAAA	1 cut(s)	847
DraIII	CACNNNGTG	1 cut(s)	168
DrdI	GACNNNNNNGTC	1 cut(s)	514
DseDI	GACNNNNNNGTC	1 cut(s)	514
EaeI	YGGCCR	1 cut(s)	552
Eam1104I	CTCTTC	4 cut(s)	23, 29, 752, 959
EarI	CTCTTC	4 cut(s)	23, 29, 752, 959
EciI	GGCGGA	2 cut(s)	104, 206
Ecl136II	GAGCTC	1 cut(s)	636
Eco130I	CCWWGG	1 cut(s)	1311
Eco24I	GRGCYC	3 cut(s)	134, 638, 1246
Eco47I	GGWCC	2 cut(s)	378, 578
Eco47III	AGCGCT	1 cut(s)	1494
Eco53kI	GAGCTC	1 cut(s)	636
Eco57I	CTGAAG	1 cut(s)	972
Eco81I	CCTNAGG	1 cut(s)	1347
Eco88I	CYCGRG	1 cut(s)	127
Eco91I	GGTNACC	1 cut(s)	1094
EcoICRI	GAGCTC	1 cut(s)	636
EcoNI	CCTNNNNNAGG	1 cut(s)	182
EcoO109I	RGGNCCY	1 cut(s)	187
EcoO65I	GGTNACC	1 cut(s)	1094
EcoRI	GAATTC	1 cut(s)	933
EcoRII	CCWGG	1 cut(s)	183
EcoT14I	CCWWGG	1 cut(s)	1311
EcoT38I	GRGCYC	3 cut(s)	134, 638, 1246
ErhI	CCWWGG	1 cut(s)	1311
Esp3I	CGTCTC	3 cut(s)	373, 535, 1863
FaeI	CATG	3 cut(s)	1043, 1145, 1502
FaqI	GGGAC	6 cut(s)	284, 364, 376, 902, 1222, 1280
FatI	CATG	3 cut(s)	1039, 1141, 1498
FauI	CCCGC	2 cut(s)	143, 249
FblI	GTMKAC	1 cut(s)	201
FokI	GGATG	9 cut(s)	20, 46, 95, 369, 782, 953, 1307, 1586, 1641
FriOI	GRGCYC	3 cut(s)	134, 638, 1246
FspBI	CTAG	5 cut(s)	284, 774, 885, 1619, 1671
GlaI	GCGC	4 cut(s)	319, 466, 1203, 1494
GsaI	CCCAGC	2 cut(s)	268, 1208
GsuI	CTGGAG	1 cut(s)	1386
HaeII	RGCGCY	1 cut(s)	1496
HaeIII	GGCC	5 cut(s)	189, 554, 1183, 1310, 1675
HapII	CCGG	8 cut(s)	128, 225, 376, 510, 551, 626, 639, 1595
HgaI	GACGC	3 cut(s)	676, 1004, 1366
HhaI	GCGC	4 cut(s)	320, 467, 1204, 1495
Hin1I	GRCGYC	2 cut(s)	420, 687
Hin1II	CATG	3 cut(s)	1043, 1145, 1502
Hin6I	GCGC	4 cut(s)	318, 465, 1202, 1493
HinP1I	GCGC	4 cut(s)	318, 465, 1202, 1493
HincII	GTYRAC	3 cut(s)	202, 507, 1428
HindII	GTYRAC	3 cut(s)	202, 507, 1428
HindIII	AAGCTT	1 cut(s)	1172
HinfI	GANTC	6 cut(s)	203, 257, 389, 515, 812, 1135
HpaII	CCGG	8 cut(s)	128, 225, 376, 510, 551, 626, 639, 1595
HphI	GGTGA	6 cut(s)	97, 524, 1033, 1088, 1564, 1599
Hpy166II	GTNNAC	6 cut(s)	202, 507, 925, 1341, 1428, 1607
Hpy188III	TCNNGA	7 cut(s)	334, 446, 938, 973, 1403, 1619, 1733
Hpy8I	GTNNAC	6 cut(s)	202, 507, 925, 1341, 1428, 1607
Hpy99I	CGWCG	6 cut(s)	203, 422, 425, 524, 692, 998
HpyAV	CCTTC	2 cut(s)	22, 347
HpyCH4III	ACNGT	3 cut(s)	85, 658, 1521
HpyCH4IV	ACGT	4 cut(s)	366, 420, 528, 561
HpyCH4V	TGCA	8 cut(s)	360, 435, 474, 493, 540, 869, 1071, 1234
HpyF3I	CTNAG	5 cut(s)	413, 617, 873, 1175, 1347
HpySE526I	ACGT	4 cut(s)	366, 420, 528, 561
Hsp92I	GRCGYC	2 cut(s)	420, 687
Hsp92II	CATG	3 cut(s)	1043, 1145, 1502
HspAI	GCGC	4 cut(s)	318, 465, 1202, 1493
KpnI	GGTACC	1 cut(s)	1890
Kzo9I	GATC	6 cut(s)	345, 582, 705, 1104, 1197, 1684
LmnI	GCTCC	5 cut(s)	137, 641, 1405, 1470, 1500
Lsp1109I	GCAGC	4 cut(s)	447, 853, 973, 1797
LweI	GCATC	2 cut(s)	347, 760
MaeI	CTAG	5 cut(s)	284, 774, 885, 1619, 1671
MaeII	ACGT	4 cut(s)	366, 420, 528, 561
MaeIII	GTNAC	5 cut(s)	1094, 1521, 1528, 1844, 1904
MalI	GATC	6 cut(s)	347, 584, 707, 1106, 1199, 1686
MboI	GATC	6 cut(s)	345, 582, 705, 1104, 1197, 1684
MboII	GAAGA	9 cut(s)	10, 16, 658, 752, 769, 976, 1040, 1138, 1575
MflI	RGATCY	1 cut(s)	345
MhlI	GDGCHC	7 cut(s)	95, 134, 596, 638, 1246, 1370, 1467
MlyI	GAGTC	5 cut(s)	197, 266, 398, 524, 1144
MmeI	TCCRAC	4 cut(s)	502, 556, 670, 1683
MroXI	GAANNNNTTC	1 cut(s)	968
MseI	TTAA	4 cut(s)	836, 840, 846, 1695
MslI	CAYNNNNRTG	4 cut(s)	482, 560, 735, 1373
MspA1I	CMGCKG	2 cut(s)	342, 1570
MspI	CCGG	8 cut(s)	128, 225, 376, 510, 551, 626, 639, 1595
MspR9I	CCNGG	6 cut(s)	128, 129, 185, 376, 551, 626
MvaI	CCWGG	1 cut(s)	185
MvnI	CGCG	4 cut(s)	318, 502, 998, 1202
NciI	CCSGG	5 cut(s)	128, 129, 376, 551, 626
NdeII	GATC	6 cut(s)	345, 582, 705, 1104, 1197, 1684
NlaIII	CATG	3 cut(s)	1043, 1145, 1502
NlaIV	GGNNCC	4 cut(s)	133, 380, 595, 1888
NmeAIII	GCCGAG	2 cut(s)	625, 962
NmuCI	GTSAC	4 cut(s)	1094, 1521, 1528, 1904
NspI	RCATGY	1 cut(s)	1043
OliI	CACNNNNGTG	1 cut(s)	1373
PacI	TTAATTAA	1 cut(s)	840
PaqCI	CACCTGC	1 cut(s)	545
PcsI	WCGNNNNNNNCGW	2 cut(s)	204, 582
PdmI	GAANNNNTTC	1 cut(s)	968
PfeI	GAWTC	1 cut(s)	812
PinAI	ACCGGT	1 cut(s)	509
Ple19I	CGATCG	1 cut(s)	585
PleI	GAGTC	5 cut(s)	197, 265, 397, 523, 1143
PpsI	GAGTC	5 cut(s)	197, 265, 397, 523, 1143
Ppu21I	YACGTR	1 cut(s)	562
Psp124BI	GAGCTC	1 cut(s)	638
Psp6I	CCWGG	1 cut(s)	183
PspEI	GGTNACC	1 cut(s)	1094
PspFI	CCCAGC	2 cut(s)	264, 1204
PspGI	CCWGG	1 cut(s)	183
PspN4I	GGNNCC	4 cut(s)	133, 380, 595, 1888
PspPI	GGNCC	3 cut(s)	187, 378, 578
PstI	CTGCAG	1 cut(s)	437
PsuI	RGATCY	1 cut(s)	345
PvuI	CGATCG	1 cut(s)	585
PvuII	CAGCTG	1 cut(s)	342
RsaI	GTAC	6 cut(s)	176, 548, 829, 926, 1418, 1888
RsaNI	GTAC	6 cut(s)	175, 547, 828, 925, 1417, 1887
RseI	CAYNNNNRTG	4 cut(s)	482, 560, 735, 1373
Rsr2I	CGGWCCG	1 cut(s)	578
RsrII	CGGWCCG	1 cut(s)	578
SacI	GAGCTC	1 cut(s)	638
SalI	GTCGAC	1 cut(s)	200
SaqAI	TTAA	4 cut(s)	836, 840, 846, 1695
Sau3AI	GATC	6 cut(s)	345, 582, 705, 1104, 1197, 1684
Sau96I	GGNCC	3 cut(s)	187, 378, 578
ScaI	AGTACT	1 cut(s)	829
SchI	GAGTC	5 cut(s)	197, 266, 398, 524, 1144
ScrFI	CCNGG	6 cut(s)	128, 129, 185, 376, 551, 626
SduI	GDGCHC	7 cut(s)	95, 134, 596, 638, 1246, 1370, 1467
SfaNI	GCATC	2 cut(s)	347, 760
SfcI	CTRYAG	2 cut(s)	433, 1812
SinI	GGWCC	2 cut(s)	378, 578
SmaI	CCCGGG	1 cut(s)	129
SmiMI	CAYNNNNRTG	4 cut(s)	482, 560, 735, 1373
SspI	AATATT	1 cut(s)	1762
SspMI	CTAG	5 cut(s)	284, 774, 885, 1619, 1671
SstI	GAGCTC	1 cut(s)	638
StyD4I	CCNGG	6 cut(s)	126, 127, 183, 374, 549, 624
StyI	CCWWGG	1 cut(s)	1311
TaaI	ACNGT	3 cut(s)	85, 658, 1521
TaiI	ACGT	4 cut(s)	369, 423, 531, 564
TaqII	GACCGA	1 cut(s)	595
TatI	WGTACW	2 cut(s)	827, 924
TauI	GCSGC	7 cut(s)	222, 330, 428, 431, 569, 687, 714
TfiI	GAWTC	1 cut(s)	812
Tru1I	TTAA	4 cut(s)	836, 840, 846, 1695
Tru9I	TTAA	4 cut(s)	836, 840, 846, 1695
TscAI	CASTG	2 cut(s)	547, 1795
TseFI	GTSAC	4 cut(s)	1094, 1521, 1528, 1904
TseI	GCWGC	4 cut(s)	435, 866, 986, 1785
Tsp45I	GTSAC	4 cut(s)	1094, 1521, 1528, 1904
TspDTI	ATGAA	2 cut(s)	753, 1139
TspGWI	ACGGA	2 cut(s)	78, 508
TspMI	CCCGGG	1 cut(s)	127
TspRI	CASTG	2 cut(s)	547, 1795
VpaK11BI	GGWCC	2 cut(s)	378, 578
XagI	CCTNNNNNAGG	1 cut(s)	182
XapI	RAATTY	4 cut(s)	743, 877, 933, 1000
XbaI	TCTAGA	1 cut(s)	1618
XceI	RCATGY	1 cut(s)	1043
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1084
XmaI	CCCGGG	1 cut(s)	127
XmiI	GTMKAC	1 cut(s)	201
XmnI	GAANNNNTTC	1 cut(s)	968
XspI	CTAG	5 cut(s)	284, 774, 885, 1619, 1671
ZraI	GACGTC	1 cut(s)	421
ZrmI	AGTACT	1 cut(s)	829

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.