MD04G1102800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0003917
Physical Location & Seq
Reverse (-)
19000463 .. 19002178
1716 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1102800.v1.1.491

Sequence Viewer

Length: 492 bp
ATGTGGCAATTATTCGCCGCAGCTGTTGCGGCCTCCACAGGGCTCCTCGCCGGAAAACACATCTTTTTCAAACCCACCGCCGATACCGATCCGCCCGCCGATCACAATGAGGACACTGCCTGCACAGGCCCCTACAATTTCTCCGTCGCGTCGGCATTTCAGTCTCAGCCGCCTCCCTGGGAGACCAGCGACTTCGAGGAGCAACGAGAAGGGACGATCTTTAGGTTTTCGAGCGAGGCGGCGTCGGGTCGCGGCGGGAGTGGGACGCGGTTCCGGTTGAGGAAGAGAACCGGTTCGATCAAGAAGAAAGGGGTTGCTGCTGAGGCTGAGAGATCTAACGGTACAGAACAGAGGAGGACTGCAAGGAGAGGTGTTGGAGTTTGCTTGAAGAGGAGGAAGACTGGGAAGAATGTAGCTGCTACCAAATGTGGATCGTCTTCTTCCAAAGGGCTATGGTCTTGGAGATTCAACATTATTTGGTTGGGGACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

164

Amino Acids

17.76

Weight (kDa)

10.28

Isoelectric Point (pI)

50.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 149, 252, 268
AclWI GGATC 2 cut(s) 83, 439
AcyI GRCGYC 1 cut(s) 242
AfaI GTAC 1 cut(s) 343
AfiI CCNNNNNNNGG 1 cut(s) 39
AgeI ACCGGT 1 cut(s) 290
AgsI TTSAA 3 cut(s) 70, 388, 469
AjnI CCWGG 1 cut(s) 176
AluBI AGCT 2 cut(s) 23, 416
AluI AGCT 2 cut(s) 23, 416
Alw26I GTCTC 2 cut(s) 168, 176
AlwI GGATC 2 cut(s) 83, 439
AoxI GGCC 2 cut(s) 30, 127
ApeKI GCWGC 3 cut(s) 20, 317, 416
AsiGI ACCGGT 1 cut(s) 290
Asp700I GAANNNNTTC 1 cut(s) 292
AspS9I GGNCC 1 cut(s) 128
BanII GRGCYC 1 cut(s) 45
BbsI GAAGAC 2 cut(s) 404, 429
BbvCI CCTCAGC 1 cut(s) 321
BbvI GCAGC 3 cut(s) 32, 304, 403
BciT130I CCWGG 1 cut(s) 178
BcoDI GTCTC 2 cut(s) 168, 176
BglII AGATCT 1 cut(s) 332
BisI GCNGC 8 cut(s) 18, 21, 30, 170, 240, 253, 318, 417
BlsI GCNGC 8 cut(s) 19, 22, 31, 171, 241, 254, 319, 418
Bme1390I CCNGG 1 cut(s) 178
BmgT120I GGNCC 1 cut(s) 128
BmiI GGNNCC 3 cut(s) 44, 130, 272
BmrFI CCNGG 1 cut(s) 178
BmrI ACTGGG 1 cut(s) 411
BmuI ACTGGG 1 cut(s) 411
BpiI GAAGAC 2 cut(s) 404, 429
Bpu10I CCTNAGC 1 cut(s) 321
BsaBI GATNNNNATC 1 cut(s) 87
BsaHI GRCGYC 1 cut(s) 242
BsaI GGTCTC 1 cut(s) 176
BsaJI CCNNGG 2 cut(s) 176, 177
BsaWI WCCGGW 2 cut(s) 273, 290
BsaXI ACNNNNNCTCC 2 cut(s) 125, 155
Bsc4I CCNNNNNNNGG 1 cut(s) 39
Bse118I RCCGGY 1 cut(s) 290
Bse1I ACTGG 1 cut(s) 406
Bse8I GATNNNNATC 1 cut(s) 87
BseBI CCWGG 1 cut(s) 178
BseDI CCNNGG 2 cut(s) 176, 177
BseJI GATNNNNATC 1 cut(s) 87
BseLI CCNNNNNNNGG 1 cut(s) 39
BseMII CTCAG 3 cut(s) 179, 312, 318
BseNI ACTGG 1 cut(s) 406
BseRI GAGGAG 4 cut(s) 35, 212, 367, 406
BseXI GCAGC 3 cut(s) 32, 304, 403
BsgI GTGCAG 1 cut(s) 106
Bsh1236I CGCG 3 cut(s) 149, 252, 268
BshFI GGCC 2 cut(s) 32, 129
BshTI ACCGGT 1 cut(s) 290
BsiSI CCGG 3 cut(s) 51, 274, 291
BslFI GGGAC 2 cut(s) 226, 277
BslI CCNNNNNNNGG 1 cut(s) 39
BsmAI GTCTC 2 cut(s) 168, 176
BsmFI GGGAC 2 cut(s) 226, 277
BsnI GGCC 2 cut(s) 32, 129
Bso31I GGTCTC 1 cut(s) 176
Bsp1286I GDGCHC 1 cut(s) 45
Bsp143I GATC 6 cut(s) 88, 100, 216, 297, 332, 431
BspANI GGCC 2 cut(s) 32, 129
BspCNI CTCAG 3 cut(s) 178, 313, 319
BspFNI CGCG 3 cut(s) 149, 252, 268
BspLI GGNNCC 3 cut(s) 44, 130, 272
BspPI GGATC 2 cut(s) 83, 439
BspTNI GGTCTC 1 cut(s) 176
BsrFI RCCGGY 1 cut(s) 290
BsrI ACTGG 1 cut(s) 406
BssAI RCCGGY 1 cut(s) 290
BssECI CCNNGG 2 cut(s) 176, 177
BssMI GATC 6 cut(s) 88, 100, 216, 297, 332, 431
BssNI GRCGYC 1 cut(s) 242
Bst2UI CCWGG 1 cut(s) 178
Bst4CI ACNGT 1 cut(s) 341
Bst6I CTCTTC 2 cut(s) 278, 383
BstACI GRCGYC 1 cut(s) 242
BstAPI GCANNNNNTGC 1 cut(s) 26
BstC8I GCNNGC 2 cut(s) 96, 121
BstDEI CTNAG 3 cut(s) 165, 321, 327
BstFNI CGCG 3 cut(s) 149, 252, 268
BstKTI GATC 6 cut(s) 91, 103, 219, 300, 335, 434
BstMAI GTCTC 2 cut(s) 168, 176
BstMBI GATC 6 cut(s) 88, 100, 216, 297, 332, 431
BstMWI GCNNNNNNNGC 3 cut(s) 26, 29, 323
BstNI CCWGG 1 cut(s) 178
BstSCI CCNGG 1 cut(s) 176
BstUI CGCG 3 cut(s) 149, 252, 268
BstV1I GCAGC 3 cut(s) 32, 304, 403
BstV2I GAAGAC 2 cut(s) 404, 429
BstX2I RGATCY 1 cut(s) 332
BstYI RGATCY 1 cut(s) 332
BsuRI GGCC 2 cut(s) 32, 129
BtsI GCAGTG 1 cut(s) 114
BtsIMutI CAGTG 1 cut(s) 114
Cac8I GCNNGC 2 cut(s) 96, 121
Cfr10I RCCGGY 1 cut(s) 290
Cfr13I GGNCC 1 cut(s) 128
CseI GACGC 3 cut(s) 138, 231, 274
Csp6I GTAC 1 cut(s) 342
CspAI ACCGGT 1 cut(s) 290
CviJI RGCY 8 cut(s) 23, 32, 43, 129, 169, 326, 416, 451
CviKI_1 RGCY 8 cut(s) 23, 32, 43, 129, 169, 326, 416, 451
CviQI GTAC 1 cut(s) 342
DdeI CTNAG 3 cut(s) 165, 321, 327
DpnI GATC 6 cut(s) 90, 102, 218, 299, 334, 433
DpnII GATC 6 cut(s) 88, 100, 216, 297, 332, 431
Eam1104I CTCTTC 2 cut(s) 278, 383
EarI CTCTTC 2 cut(s) 278, 383
EciI GGCGGA 1 cut(s) 81
Eco24I GRGCYC 1 cut(s) 45
Eco31I GGTCTC 1 cut(s) 176
EcoO109I RGGNCCY 1 cut(s) 128
EcoRII CCWGG 1 cut(s) 176
EcoT38I GRGCYC 1 cut(s) 45
FaiI YATR 1 cut(s) 454
FaqI GGGAC 2 cut(s) 226, 277
FauI CCCGC 2 cut(s) 103, 248
Fnu4HI GCNGC 8 cut(s) 18, 21, 30, 170, 240, 253, 318, 417
FriOI GRGCYC 1 cut(s) 45
Fsp4HI GCNGC 8 cut(s) 18, 21, 30, 170, 240, 253, 318, 417
GluI GCNGC 8 cut(s) 18, 21, 30, 170, 240, 253, 318, 417
HaeIII GGCC 2 cut(s) 32, 129
HapII CCGG 3 cut(s) 51, 274, 291
HgaI GACGC 3 cut(s) 138, 231, 274
Hin1I GRCGYC 1 cut(s) 242
HinfI GANTC 1 cut(s) 465
HpaII CCGG 3 cut(s) 51, 274, 291
Hpy188III TCNNGA 1 cut(s) 301
Hpy99I CGWCG 3 cut(s) 149, 154, 247
HpyAV CCTTC 1 cut(s) 203
HpyCH4III ACNGT 1 cut(s) 341
HpyCH4V TGCA 2 cut(s) 123, 362
HpyF10VI GCNNNNNNNGC 3 cut(s) 26, 29, 323
HpyF3I CTNAG 3 cut(s) 165, 321, 327
Hsp92I GRCGYC 1 cut(s) 242
Kzo9I GATC 6 cut(s) 88, 100, 216, 297, 332, 431
LmnI GCTCC 2 cut(s) 48, 199
Lsp1109I GCAGC 3 cut(s) 32, 304, 403
MalI GATC 6 cut(s) 90, 102, 218, 299, 334, 433
MboI GATC 6 cut(s) 88, 100, 216, 297, 332, 431
MboII GAAGA 7 cut(s) 295, 316, 400, 409, 418, 429, 432
MflI RGATCY 1 cut(s) 332
MhlI GDGCHC 1 cut(s) 45
MluCI AATT 2 cut(s) 8, 136
MmeI TCCRAC 1 cut(s) 355
MroXI GAANNNNTTC 1 cut(s) 292
MseI TTAA 1 cut(s) 490
MspA1I CMGCKG 1 cut(s) 23
MspI CCGG 3 cut(s) 51, 274, 291
MspR9I CCNGG 1 cut(s) 178
MvaI CCWGG 1 cut(s) 178
MvnI CGCG 3 cut(s) 149, 252, 268
MwoI GCNNNNNNNGC 3 cut(s) 26, 29, 323
NdeII GATC 6 cut(s) 88, 100, 216, 297, 332, 431
NlaIV GGNNCC 3 cut(s) 44, 130, 272
PasI CCCWGGG 1 cut(s) 177
PdmI GAANNNNTTC 1 cut(s) 292
PfeI GAWTC 1 cut(s) 465
PinAI ACCGGT 1 cut(s) 290
PkrI GCNGC 8 cut(s) 19, 22, 31, 171, 241, 254, 319, 418
Psp6I CCWGG 1 cut(s) 176
PspGI CCWGG 1 cut(s) 176
PspN4I GGNNCC 3 cut(s) 44, 130, 272
PspPI GGNCC 1 cut(s) 128
PsuI RGATCY 1 cut(s) 332
PvuII CAGCTG 1 cut(s) 23
RsaI GTAC 1 cut(s) 343
RsaNI GTAC 1 cut(s) 342
SaqAI TTAA 1 cut(s) 490
SatI GCNGC 8 cut(s) 18, 21, 30, 170, 240, 253, 318, 417
Sau3AI GATC 6 cut(s) 88, 100, 216, 297, 332, 431
Sau96I GGNCC 1 cut(s) 128
ScrFI CCNGG 1 cut(s) 178
SduI GDGCHC 1 cut(s) 45
SetI ASST 4 cut(s) 25, 227, 373, 418
Sse9I AATT 2 cut(s) 8, 136
StyD4I CCNGG 1 cut(s) 176
TaaI ACNGT 1 cut(s) 341
TaqI TCGA 3 cut(s) 195, 230, 296
TasI AATT 2 cut(s) 8, 136
TauI GCSGC 5 cut(s) 20, 32, 172, 242, 255
TfiI GAWTC 1 cut(s) 465
Tru1I TTAA 1 cut(s) 490
Tru9I TTAA 1 cut(s) 490
TscAI CASTG 1 cut(s) 121
TseI GCWGC 3 cut(s) 20, 317, 416
TspGWI ACGGA 1 cut(s) 133
TspRI CASTG 1 cut(s) 121
XmnI GAANNNNTTC 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.