Rroxscaffold_6G00412380

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0003917
Physical Location & Seq
Reverse (-)
34968925 .. 34972117
3193 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00412380.1

Sequence Viewer

Length: 1251 bp
ATGTGGCCGGCTCTCTTCGCCGCAGCAGTCGCCGCCTCCACCGGACTCCTCGCCAAAAACCACCTCTTCAAGCCCGCCGCCGAGATCACCCACTCCGATCCGCAGAACGACGCCGTCTCGGACGATGCGACGCCTCAGCGCTCTGTCGCTTCTGTTGCTCGGTCTCCGGTGCTGCCGTGGGAGAGCGACTGCGAGGATCAGGTCAAGGACGGGATCTTTAGGTTTTCCAGCGACGGCGGGAGCGGGAAGAAGAAGAAGAAGAAGAAGACCAGGTTTCGGTTGAAGAAGAAAGATGTGGTGGAGGAGCAGAAGAAGCAGAGGGGTAGGAGAGTTGGTGTTTGCTTGAAGAAGAGGAAGACTGTAGCTTCCAAATGTGGATCGTCTTCTGATTCCAAAGATACCAGCTCATTGTTTAGTTGGGGACTTAACATTGGGATCATGTACATGATGACTGCTGGAAAAGCGGAAATCAATAAGCTGAACACAACCATGGATGAGACTGCAAAAGTTGTGCAGGAGTTAAAATCTGAGCTAAATAAAAGAAAAGCATCACAGAGTCAGCAAGTTTCTTGTTCTGAAAGTGAAGCCAATACACAAGTTCAGAGCCCTAGTTGCAAGCGTACTCAGACAGAACTCAAGAAGTCAAGTGCAGAAAACAGTGAACCTAATTATATGAGAGCTTCCAGTTTTCATATATTTGATGATGGTGAATGTCCAAGTAGTGTTCTTACAGAGGAACAGGAGCCTGAGCCAGAGGTGATGGACATGGATCAATTGGAAGCAGAACTTGAGTCTGAACTGCAAAAACTTCCATGGTGCTCCACAGAAGCTCCTCATCAAGAGGGATTCAGAAATCTGGGAAAGGATATTGTCTCTGAAGTCTCAGCACAAAAATTTCAGGGACAAGCAGCTCAAGGTATTGACATCGAGCAATTTCAAGGAGTATTACCAGCTGAACTAGACCAGAAGTTATGCCATGTGCTCATCGAACAGCAGGAAAGCCAAATTGTGGAACTGGAATCCGAACTGCAGTCGGCCCAATCCAAACTCCAAGAAAAAGAAACTGAACTTCAAGCATTGAAGGACTGTGTTAAACGCCTAACTCAGTTATCCCTTTCAACTGTCTCAGATGATGAAAATGAGGCTCACAATGAACAAGAGCAGATCACTGGTTGGAATTACAACACGGAGAGATCTGAGTCAATAAAACCAGTGGTTGGGATGAAAAGACCTATAGATTCTGAATCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

416

Amino Acids

46.28

Weight (kDa)

5.28

Isoelectric Point (pI)

67.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 1009, 1217
AccBSI CCGCTC 1 cut(s) 243
AciI CCGC 8 cut(s) 21, 33, 75, 78, 101, 237, 243, 464
AclWI GGATC 6 cut(s) 92, 204, 221, 385, 443, 777
AcoI YGGCCR 1 cut(s) 5
AcsI RAATTY 1 cut(s) 893
AcuI CTGAAG 1 cut(s) 897
AcyI GRCGYC 2 cut(s) 111, 131
AfaI GTAC 2 cut(s) 443, 622
AfeI AGCGCT 1 cut(s) 140
AfiI CCNNNNNNNGG 3 cut(s) 276, 1009, 1217
AgsI TTSAA 7 cut(s) 70, 283, 346, 938, 1073, 1081, 1119
AjnI CCWGG 1 cut(s) 269
AluBI AGCT 8 cut(s) 365, 405, 478, 532, 680, 830, 911, 953
AluI AGCT 8 cut(s) 365, 405, 478, 532, 680, 830, 911, 953
Alw21I GWGCWC 2 cut(s) 821, 984
Alw26I GTCTC 6 cut(s) 121, 168, 491, 877, 886, 1129
AlwI GGATC 6 cut(s) 92, 204, 221, 385, 443, 777
Aor51HI AGCGCT 1 cut(s) 140
AoxI GGCC 2 cut(s) 5, 1035
ApeKI GCWGC 3 cut(s) 23, 172, 908
ApoI RAATTY 1 cut(s) 893
AspLEI GCGC 1 cut(s) 141
AspS9I GGNCC 1 cut(s) 1036
AsuHPI GGTGA 3 cut(s) 79, 719, 769
BanII GRGCYC 1 cut(s) 608
BbsI GAAGAC 3 cut(s) 272, 362, 375
Bbv12I GWGCWC 2 cut(s) 821, 984
BbvCI CCTCAGC 1 cut(s) 135
BbvI GCAGC 3 cut(s) 35, 159, 920
BccI CCATC 2 cut(s) 698, 754
BceAI ACGGC 3 cut(s) 98, 160, 250
BciT130I CCWGG 1 cut(s) 271
BcoDI GTCTC 6 cut(s) 121, 168, 491, 877, 886, 1129
BfaI CTAG 2 cut(s) 609, 959
BfmI CTRYAG 3 cut(s) 360, 1028, 1233
BfoI RGCGCY 1 cut(s) 142
BglII AGATCT 1 cut(s) 1193
BisI GCNGC 6 cut(s) 21, 24, 33, 78, 173, 909
BlsI GCNGC 6 cut(s) 22, 25, 34, 79, 174, 910
Bme1390I CCNGG 1 cut(s) 271
BmgT120I GGNCC 1 cut(s) 1036
BmiI GGNNCC 1 cut(s) 744
BmrFI CCNGG 1 cut(s) 271
BmsI GCATC 2 cut(s) 115, 557
BpiI GAAGAC 3 cut(s) 272, 362, 375
Bpu10I CCTNAGC 2 cut(s) 135, 747
BpuEI CTTGAG 3 cut(s) 620, 809, 897
BsaHI GRCGYC 2 cut(s) 111, 131
BsaI GGTCTC 1 cut(s) 168
BsaJI CCNNGG 3 cut(s) 176, 489, 812
BsaWI WCCGGW 2 cut(s) 41, 166
BsaXI ACNNNNNCTCC 4 cut(s) 319, 349, 814, 844
Bsc4I CCNNNNNNNGG 3 cut(s) 276, 1009, 1217
Bse118I RCCGGY 1 cut(s) 7
Bse1I ACTGG 4 cut(s) 684, 1020, 1174, 1211
BseBI CCWGG 1 cut(s) 271
BseDI CCNNGG 3 cut(s) 176, 489, 812
BseGI GGATG 2 cut(s) 499, 1227
BseLI CCNNNNNNNGG 3 cut(s) 276, 1009, 1217
BseMII CTCAG 8 cut(s) 149, 519, 638, 738, 897, 1118, 1140, 1188
BseNI ACTGG 4 cut(s) 684, 1020, 1174, 1211
BseRI GAGGAG 3 cut(s) 38, 317, 822
BseXI GCAGC 3 cut(s) 35, 159, 920
BsgI GTGCAG 2 cut(s) 533, 669
BshFI GGCC 2 cut(s) 7, 1037
BsiHKAI GWGCWC 2 cut(s) 821, 984
BsiSI CCGG 3 cut(s) 8, 42, 167
BslFI GGGAC 2 cut(s) 435, 915
BslI CCNNNNNNNGG 3 cut(s) 276, 1009, 1217
BsmAI GTCTC 6 cut(s) 121, 168, 491, 877, 886, 1129
BsmBI CGTCTC 1 cut(s) 121
BsmFI GGGAC 2 cut(s) 435, 915
BsnI GGCC 2 cut(s) 7, 1037
Bso31I GGTCTC 1 cut(s) 168
Bsp1286I GDGCHC 3 cut(s) 608, 821, 984
Bsp1407I TGTACA 1 cut(s) 441
Bsp143I GATC 9 cut(s) 84, 97, 196, 213, 377, 435, 769, 1164, 1193
Bsp19I CCATGG 2 cut(s) 489, 812
BspACI CCGC 8 cut(s) 21, 33, 75, 78, 101, 237, 243, 464
BspANI GGCC 2 cut(s) 7, 1037
BspCNI CTCAG 8 cut(s) 148, 520, 637, 739, 896, 1117, 1139, 1189
BspLI GGNNCC 1 cut(s) 744
BspMAI CTGCAG 1 cut(s) 1032
BspPI GGATC 6 cut(s) 92, 204, 221, 385, 443, 777
BspTNI GGTCTC 1 cut(s) 168
BsrBI CCGCTC 1 cut(s) 243
BsrFI RCCGGY 1 cut(s) 7
BsrGI TGTACA 1 cut(s) 441
BsrI ACTGG 4 cut(s) 684, 1020, 1174, 1211
BssAI RCCGGY 1 cut(s) 7
BssECI CCNNGG 3 cut(s) 176, 489, 812
BssMI GATC 9 cut(s) 84, 97, 196, 213, 377, 435, 769, 1164, 1193
BssNI GRCGYC 2 cut(s) 111, 131
BssT1I CCWWGG 2 cut(s) 489, 812
Bst2UI CCWGG 1 cut(s) 271
Bst4CI ACNGT 4 cut(s) 361, 659, 1088, 1123
Bst6I CTCTTC 3 cut(s) 20, 71, 344
BstACI GRCGYC 2 cut(s) 111, 131
BstAUI TGTACA 1 cut(s) 441
BstC8I GCNNGC 3 cut(s) 9, 75, 617
BstDEI CTNAG 8 cut(s) 135, 528, 624, 747, 883, 1104, 1126, 1197
BstDSI CCRYGG 3 cut(s) 176, 489, 812
BstF5I GGATG 2 cut(s) 499, 1227
BstH2I RGCGCY 1 cut(s) 142
BstHHI GCGC 1 cut(s) 141
BstKTI GATC 9 cut(s) 87, 100, 199, 216, 380, 438, 772, 1167, 1196
BstMAI GTCTC 6 cut(s) 121, 168, 491, 877, 886, 1129
BstMBI GATC 9 cut(s) 84, 97, 196, 213, 377, 435, 769, 1164, 1193
BstMWI GCNNNNNNNGC 7 cut(s) 17, 29, 32, 155, 313, 461, 612
BstNI CCWGG 1 cut(s) 271
BstSCI CCNGG 1 cut(s) 269
BstSFI CTRYAG 3 cut(s) 360, 1028, 1233
BstV1I GCAGC 3 cut(s) 35, 159, 920
BstV2I GAAGAC 3 cut(s) 272, 362, 375
BstX2I RGATCY 2 cut(s) 213, 1193
BstYI RGATCY 2 cut(s) 213, 1193
BsuRI GGCC 2 cut(s) 7, 1037
BtgI CCRYGG 3 cut(s) 176, 489, 812
BtsCI GGATG 2 cut(s) 499, 1227
BtsIMutI CAGTG 3 cut(s) 664, 1167, 1218
Cac8I GCNNGC 3 cut(s) 9, 75, 617
CfoI GCGC 1 cut(s) 141
Cfr10I RCCGGY 1 cut(s) 7
Cfr13I GGNCC 1 cut(s) 1036
CseI GACGC 2 cut(s) 119, 139
CsiI ACCWGGT 1 cut(s) 269
Csp6I GTAC 2 cut(s) 442, 621
CviAII CATG 6 cut(s) 439, 445, 490, 766, 813, 977
CviQI GTAC 2 cut(s) 442, 621
DdeI CTNAG 8 cut(s) 135, 528, 624, 747, 883, 1104, 1126, 1197
DpnI GATC 9 cut(s) 86, 99, 198, 215, 379, 437, 771, 1166, 1195
DpnII GATC 9 cut(s) 84, 97, 196, 213, 377, 435, 769, 1164, 1193
EaeI YGGCCR 1 cut(s) 5
Eam1104I CTCTTC 3 cut(s) 20, 71, 344
EarI CTCTTC 3 cut(s) 20, 71, 344
Eco130I CCWWGG 2 cut(s) 489, 812
Eco24I GRGCYC 1 cut(s) 608
Eco31I GGTCTC 1 cut(s) 168
Eco47III AGCGCT 1 cut(s) 140
Eco57I CTGAAG 1 cut(s) 897
EcoRII CCWGG 1 cut(s) 269
EcoT14I CCWWGG 2 cut(s) 489, 812
EcoT38I GRGCYC 1 cut(s) 608
ErhI CCWWGG 2 cut(s) 489, 812
Esp3I CGTCTC 1 cut(s) 121
FaeI CATG 6 cut(s) 442, 448, 493, 769, 816, 980
FalI AAGNNNNNCTT 2 cut(s) 771, 803
FaqI GGGAC 2 cut(s) 435, 915
FatI CATG 6 cut(s) 438, 444, 489, 765, 812, 976
FauI CCCGC 3 cut(s) 82, 230, 236
Fnu4HI GCNGC 6 cut(s) 21, 24, 33, 78, 173, 909
FokI GGATG 2 cut(s) 506, 1234
FriOI GRGCYC 1 cut(s) 608
Fsp4HI GCNGC 6 cut(s) 21, 24, 33, 78, 173, 909
FspBI CTAG 2 cut(s) 609, 959
GlaI GCGC 1 cut(s) 140
GluI GCNGC 6 cut(s) 21, 24, 33, 78, 173, 909
HaeII RGCGCY 1 cut(s) 142
HaeIII GGCC 2 cut(s) 7, 1037
HapII CCGG 3 cut(s) 8, 42, 167
HgaI GACGC 2 cut(s) 119, 139
HhaI GCGC 1 cut(s) 141
Hin1I GRCGYC 2 cut(s) 111, 131
Hin1II CATG 6 cut(s) 442, 448, 493, 769, 816, 980
Hin6I GCGC 1 cut(s) 139
HinP1I GCGC 1 cut(s) 139
HinfI GANTC 9 cut(s) 45, 389, 556, 791, 846, 1019, 1199, 1238, 1244
HpaII CCGG 3 cut(s) 8, 42, 167
HphI GGTGA 3 cut(s) 79, 719, 769
Hpy166II GTNNAC 1 cut(s) 662
Hpy188III TCNNGA 3 cut(s) 637, 839, 1248
Hpy8I GTNNAC 1 cut(s) 662
Hpy99I CGWCG 3 cut(s) 113, 133, 236
HpyAV CCTTC 1 cut(s) 1075
HpyCH4III ACNGT 4 cut(s) 361, 659, 1088, 1123
HpyCH4V TGCA 6 cut(s) 503, 514, 615, 650, 802, 1030
HpyF10VI GCNNNNNNNGC 7 cut(s) 17, 29, 32, 155, 313, 461, 612
HpyF3I CTNAG 8 cut(s) 135, 528, 624, 747, 883, 1104, 1126, 1197
Hsp92I GRCGYC 2 cut(s) 111, 131
Hsp92II CATG 6 cut(s) 442, 448, 493, 769, 816, 980
HspAI GCGC 1 cut(s) 139
KroI GCCGGC 1 cut(s) 7
KroNI GCCGGC 1 cut(s) 9
Kzo9I GATC 9 cut(s) 84, 97, 196, 213, 377, 435, 769, 1164, 1193
LmnI GCTCC 5 cut(s) 240, 304, 742, 824, 835
Lsp1109I GCAGC 3 cut(s) 35, 159, 920
LweI GCATC 2 cut(s) 115, 557
MabI ACCWGGT 1 cut(s) 269
MaeI CTAG 2 cut(s) 609, 959
MalI GATC 9 cut(s) 86, 99, 198, 215, 379, 437, 771, 1166, 1195
MbiI CCGCTC 1 cut(s) 243
MboI GATC 9 cut(s) 84, 97, 196, 213, 377, 435, 769, 1164, 1193
MfeI CAATTG 1 cut(s) 773
MflI RGATCY 2 cut(s) 213, 1193
MhlI GDGCHC 3 cut(s) 608, 821, 984
MluCI AATT 6 cut(s) 667, 773, 893, 932, 1005, 1177
MlyI GAGTC 4 cut(s) 39, 565, 800, 1208
MmeI TCCRAC 1 cut(s) 1154
MroNI GCCGGC 1 cut(s) 7
MseI TTAA 3 cut(s) 426, 521, 1092
MslI CAYNNNNRTG 2 cut(s) 443, 488
MspA1I CMGCKG 1 cut(s) 953
MspI CCGG 3 cut(s) 8, 42, 167
MspR9I CCNGG 1 cut(s) 271
MunI CAATTG 1 cut(s) 773
MvaI CCWGG 1 cut(s) 271
MwoI GCNNNNNNNGC 7 cut(s) 17, 29, 32, 155, 313, 461, 612
NaeI GCCGGC 1 cut(s) 9
NcoI CCATGG 2 cut(s) 489, 812
NdeII GATC 9 cut(s) 84, 97, 196, 213, 377, 435, 769, 1164, 1193
NgoMIV GCCGGC 1 cut(s) 7
NlaIII CATG 6 cut(s) 442, 448, 493, 769, 816, 980
NlaIV GGNNCC 1 cut(s) 744
NmeAIII GCCGAG 1 cut(s) 106
PcsI WCGNNNNNNNCGW 1 cut(s) 125
PdiI GCCGGC 1 cut(s) 9
PfeI GAWTC 5 cut(s) 389, 846, 1019, 1238, 1244
PflFI GACNNNGTC 1 cut(s) 113
PflMI CCANNNNNTGG 2 cut(s) 1009, 1217
PkrI GCNGC 6 cut(s) 22, 25, 34, 79, 174, 910
PleI GAGTC 4 cut(s) 39, 564, 799, 1207
PpsI GAGTC 4 cut(s) 39, 564, 799, 1207
Psp6I CCWGG 1 cut(s) 269
PspGI CCWGG 1 cut(s) 269
PspN4I GGNNCC 1 cut(s) 744
PspPI GGNCC 1 cut(s) 1036
PstI CTGCAG 1 cut(s) 1032
PsuI RGATCY 2 cut(s) 213, 1193
PsyI GACNNNGTC 1 cut(s) 113
PvuII CAGCTG 1 cut(s) 953
RsaI GTAC 2 cut(s) 443, 622
RsaNI GTAC 2 cut(s) 442, 621
RseI CAYNNNNRTG 2 cut(s) 443, 488
SaqAI TTAA 3 cut(s) 426, 521, 1092
SatI GCNGC 6 cut(s) 21, 24, 33, 78, 173, 909
Sau3AI GATC 9 cut(s) 84, 97, 196, 213, 377, 435, 769, 1164, 1193
Sau96I GGNCC 1 cut(s) 1036
SchI GAGTC 4 cut(s) 39, 565, 800, 1208
ScrFI CCNGG 1 cut(s) 271
SduI GDGCHC 3 cut(s) 608, 821, 984
SexAI ACCWGGT 1 cut(s) 269
SfaNI GCATC 2 cut(s) 115, 557
SfcI CTRYAG 3 cut(s) 360, 1028, 1233
SmiMI CAYNNNNRTG 2 cut(s) 443, 488
SmlI CTYRAG 3 cut(s) 635, 788, 912
SmoI CTYRAG 3 cut(s) 635, 788, 912
Sse9I AATT 6 cut(s) 667, 773, 893, 932, 1005, 1177
SsiI CCGC 8 cut(s) 21, 33, 75, 78, 101, 237, 243, 464
SspMI CTAG 2 cut(s) 609, 959
StyD4I CCNGG 1 cut(s) 269
StyI CCWWGG 2 cut(s) 489, 812
TaaI ACNGT 4 cut(s) 361, 659, 1088, 1123
TaqI TCGA 2 cut(s) 927, 987
TaqII GACCGA 1 cut(s) 150
TasI AATT 6 cut(s) 667, 773, 893, 932, 1005, 1177
TatI WGTACW 1 cut(s) 441
TauI GCSGC 3 cut(s) 23, 35, 80
TfiI GAWTC 5 cut(s) 389, 846, 1019, 1238, 1244
Tru1I TTAA 3 cut(s) 426, 521, 1092
Tru9I TTAA 3 cut(s) 426, 521, 1092
TscAI CASTG 3 cut(s) 664, 1174, 1218
TseI GCWGC 3 cut(s) 23, 172, 908
TspDTI ATGAA 4 cut(s) 680, 1149, 1167, 1238
TspGWI ACGGA 1 cut(s) 1202
TspRI CASTG 3 cut(s) 664, 1174, 1218
Tth111I GACNNNGTC 1 cut(s) 113
Van91I CCANNNNNTGG 2 cut(s) 1009, 1217
XapI RAATTY 1 cut(s) 893
XspI CTAG 2 cut(s) 609, 959
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.