Rorug03G0098200

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000085
Physical Location & Seq
Forward (+)
7692853 .. 7696837
3985 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0098200.1

Sequence Viewer

Length: 2865 bp
ATGTCACTCAAACTCGTCTCTTTCCTTCTTCTCTGGTCACCTGTGTTGGTACTTTCCTTAAACCCTGTTTTCAACGACGACGTTTTGGGGCTGATCGTCTTCAAGGCCGGGCTGCTAGACCCGGAGGCCAAGCTCGGTTCTTGGAACGAAGAGGATGACAGTCCTTGCAATTGGGTCGGTGTCAAATGCGACCCCAGAAGCAACAGAGTCTCTGAGCTTGCTCTAGATGGGTTTGGCCTGTCTGGGCATGTAAACCGAGGCCTTTTGAGGTTGCAGTTCATTCAGAGACTCTCACTTTCTAACAACAACTTCACTGGGTCTATAAACCCTGATCTGGCTCACATTGGGACTTTGCAAGTCATTGATTTGAGCCAGAATGGCCTCTCCGGGTCGATCCCGGATGAGTTTTTTCAACAATGTGGGTCACTGAGAGATGTTTCGTTTGCGAAAAACAATCTCTCTGGGAGAATCCCCGAGTCTCTGAGCTTCTGCTCTGCTTTGGTGGCGGTGAACTTTTCGTCGAATCAGCTTTCGGGGAAGTTACCATCTGGGATATGGTATTTGAGGGGGCTTCGGAAGCTGGACTTGTCAGGCAACTTGCTGGAGGGTGATGTTCATGAAGGTATTGGATATTTGTATGATTTGAGACTAGTTAACTTGGGGAGAAATCGGTTTTCGGGGTGGCTTCCCCAGGATGTTGGAGGCTGTTCACATTTGAAGTTGCTTGATTTCAGTGATAATTTATTTTCCGGGGGAATTCCTGAGTCGATTAAGAGACTTGGTTTGTGTAGGTCTTTGAGTTTAAAGGGGAACTCTCTTACGGGTCAAGTTCCTGCTTGGATTGGTGAGTTGAGAAGGCTTGGGATGTTAGATTTGTCTTGTAATAACTTTTCTGGTGGAATTCCTGGTTCATTAGGAAATCTAAGGCTACTTGAGAAGTTGAATTTATCTATGAATGGATTTACTGGGAGCTTGCCTGAGTCGTTGACAAATTGTTTCAACCTTCTAGCTCTAGATGTTAGCCGAAATCAGTTGGTTGGTAAACTTCCTTCATGGATTTTGAAGCTTGGTGTAGGACATGGAAGGCTCGAGTACAATCCATTGAGACCAATAGCTGCCTCTCATGGACGTCTTCAGGTCTTGGATTTGTCTTCCAATGCATTTTCTGATGTGCTTCCATCTGATATTGGGGTTCTCAGTAGCTTGCAGTTCTTGAATGTGTCCCGGAATCAGCTCCTTGGTACTATTCCTGTAAGTATAGGTAATTTGACGTCAGCATATGTTCTGGACTTGAGCAGCAATTGGCTGAATGGAAGCATTCCTTCTGAAATTGGAGGAGCAGTCTCACTCAAGGAGCTAAGGCTGCACAAGAACTTTCTAACGGGGAAAGTTCCATCGCAGATTGAGAAGTGCTCATCTCTATCATCGCTGTTGCTATCCCAGAACAATCTTACTGGCCCTGTTCCTGCAGCCATTGCAAACCTTACCAATCTTCAATACGTCGACTTGTCTTTAAACCAATTCTCTGGGAGCTTGCCTAAAGAGTTGACAAATCTTTCCCACCTCCTCTACTTCAATGTATCCTACAATCACCTTCAGGGTGAGTTACCAGTGGGGGGCTTCTTCAACACAATCTCACCCTCATCTATCTCGGGCAATCCGTCCCTATGTGGTTCTGTTCTTAACCTTTCATGCCCTGCTGTTCACCCTAAACCCATTGTCCTCAACCCAAATTCTAACAACACTGGTGGTGGTTCTTCCTCTCTGACTCATGGTCACAAGAATGTATTTAGCATATCTGCTCTCATTGCCATAGGTGCTGCTGCATTCATTGCAATTGGTGTTATAGCTGTTACCGTCCTCAATATGCATGTGCGGTCTTCAATGACACATTCTGCTCCTCTTCCACTACCTGGTGGGGAAGATTTCAGTTGTTCCCCAAGTACTGATTCAAAGTATGGCAAGCTTGTTATGTTTTCTGGTGATGCTGACTTTGCTGCTGGAGCCCAAGCCCTGCTGAACAAGGACTGTGAGCTTGGTCGTGGTGGCTTTGGAGTAGTTTACAGAACGGTCCTCAGAGATGGGCGTTCAGTTGCAATAAAAAAGTTGACAGTCTCAAGTTTGATCAAGTCCCAAGAAGATTTCGAGGGGGAAGTTAAAGGACTTGGAAAGATAAGGCACCATAATCTTGTGACACTTGAAGGGTATTACTGGACTCCTTCCTTGCAGCTGCTCATATATGAATACATACCATCTGGAAGTCTGTATAAGAATCTGCATGATGGACCTGGCCAAAGCGGTCTCTCTTGGCGACAGAGGTTCAACATAATTCTTGGGATGGCAAAGGGTTTGGCTCATCTGCATCGAATGAACATGATTCACTACAACTTGAAATCAACTAATATTTTGATAGACAGCTCTGGTGAACCAAAAGTAGGAGACTTTAGTCTGGCAAGGCTGTTGCCAATGTTAGACCGGTGCATCTTGAGCAGCAAAATCCAAAGTGCACTTGGGTATATGGCGCCTGAGTTTGCTTGTCAAACTGTCAAGATTACTGAGAAATGTGATGTGTATGGGTACGGGGTCTTGGTTTTGGAGGTGGTGACCGGAAAGCGGCCTGTGGAATATATGGAAGATGATGTGGTGGTGCTCTGTGACATGGTGAGGGGGGCTCTAGAGGAAGGCAGGGTGGAAGAATGTCTTGACAGAAAACTTCTGGGTAATTATCCGGCAGAGGAGGCAATTCCAGTGATCAAGCTTGGTTTGATATGTGCATCACAAGTTCCATCAAATCGTCCCGATATGAGTGAAGTAGTCAACATCTTAGAACTCATCCAATGCCCTTCAGAAGGCCATGAAGATTAG

Protein Analysis

954

Amino Acids

103.05

Weight (kDa)

6.42

Isoelectric Point (pI)

39.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 25 - 64 1.1e-12 Leucine rich repeat N-terminal domain
LRR_14 PF23598 189 - 336 3.6e-09 Leucine-rich repeat region
LRR_8 PF13855 264 - 320 4.4e-06 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 675 - 941 1e-39 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 676 - 880 2.6e-37 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 1132, 1274
AccB1I GGYRCC 2 cut(s) 2178, 2521
AccB7I CCANNNNNTGG 1 cut(s) 1913
AccI GTMKAC 1 cut(s) 1503
AciI CCGC 4 cut(s) 506, 1876, 2298, 2614
AclWI GGATC 1 cut(s) 388
AcoI YGGCCR 1 cut(s) 2290
AcsI RAATTY 4 cut(s) 756, 900, 943, 1732
AcuI CTGAAG 3 cut(s) 1118, 1580, 2829
AcyI GRCGYC 3 cut(s) 1129, 1271, 2522
AfaI GTAC 5 cut(s) 51, 1094, 1243, 1945, 2579
AfiI CCNNNNNNNGG 6 cut(s) 334, 1616, 1913, 2435, 2613, 2849
AgeI ACCGGT 1 cut(s) 2475
AhdI GACNNNNNGTC 1 cut(s) 1773
AhlI ACTAGT 1 cut(s) 649
AjnI CCWGG 4 cut(s) 690, 904, 1912, 2287
AjuI GAANNNNNNNTTGG 2 cut(s) 1933, 1965
Alw21I GWGCWC 3 cut(s) 1415, 2509, 2652
Alw44I GTGCAC 1 cut(s) 2505
AlwI GGATC 1 cut(s) 388
Ama87I CYCGRG 3 cut(s) 473, 1088, 1651
AoxI GGCC 9 cut(s) 105, 126, 235, 259, 379, 1456, 2290, 2615, 2851
ApaLI GTGCAC 1 cut(s) 2505
ApoI RAATTY 4 cut(s) 756, 900, 943, 1732
AsiGI ACCGGT 1 cut(s) 2475
AspLEI GCGC 1 cut(s) 2524
AspS9I GGNCC 3 cut(s) 1457, 2071, 2285
AsuC2I CCSGG 6 cut(s) 109, 122, 388, 398, 751, 1225
AvaI CYCGRG 3 cut(s) 473, 1088, 1651
AvaII GGWCC 2 cut(s) 2071, 2285
BaeGI GKGCMC 1 cut(s) 2509
BalI TGGCCA 1 cut(s) 2292
BanI GGYRCC 2 cut(s) 2178, 2521
BanII GRGCYC 2 cut(s) 2008, 2674
BarI GAAGNNNNNNTAC 2 cut(s) 1033, 1065
BbsI GAAGAC 4 cut(s) 91, 1124, 1143, 1872
Bbv12I GWGCWC 3 cut(s) 1415, 2509, 2652
BccI CCATC 9 cut(s) 221, 553, 1186, 1402, 2075, 2260, 2276, 2332, 2794
BcgI CGANNNNNNTGC 2 cut(s) 157, 191
BciT130I CCWGG 4 cut(s) 692, 906, 1914, 2289
BciVI GTATCC 1 cut(s) 1591
BclI TGATCA 2 cut(s) 2124, 2751
BcnI CCSGG 6 cut(s) 109, 122, 388, 398, 751, 1225
BcuI ACTAGT 1 cut(s) 649
BfaI CTAG 6 cut(s) 116, 224, 650, 1007, 1013, 2675
BfmI CTRYAG 1 cut(s) 1467
BfoI RGCGCY 1 cut(s) 2525
BfuI GTATCC 1 cut(s) 1591
BglI GCCNNNNNGGC 1 cut(s) 378
BmcAI AGTACT 1 cut(s) 1945
Bme18I GGWCC 2 cut(s) 2071, 2285
BmeRI GACNNNNNGTC 1 cut(s) 1773
BmeT110I CYCGRG 3 cut(s) 473, 1088, 1651
BmgT120I GGNCC 3 cut(s) 1457, 2071, 2285
BmiI GGNNCC 3 cut(s) 2005, 2180, 2523
BmrI ACTGGG 2 cut(s) 324, 975
BmsI GCATC 4 cut(s) 1975, 2371, 2490, 2783
BmuI ACTGGG 2 cut(s) 324, 975
BoxI GACNNNNGTC 1 cut(s) 2445
BpiI GAAGAC 4 cut(s) 91, 1124, 1143, 1872
BplI GAGNNNNNCTC 4 cut(s) 1397, 1429, 2656, 2688
BpmI CTGGAG 2 cut(s) 623, 2022
Bpu10I CCTNAGC 1 cut(s) 1358
BpuEI CTTGAG 5 cut(s) 953, 1312, 1334, 2101, 2506
BpuMI CCSGG 6 cut(s) 109, 122, 388, 398, 751, 1225
BsaHI GRCGYC 3 cut(s) 1129, 1271, 2522
BsaI GGTCTC 2 cut(s) 1099, 2306
BsaJI CCNNGG 4 cut(s) 256, 690, 750, 1237
BsaWI WCCGGW 2 cut(s) 2475, 2606
BsaXI ACNNNNNCTCC 2 cut(s) 1326, 1356
Bsc4I CCNNNNNNNGG 6 cut(s) 334, 1616, 1913, 2435, 2613, 2849
Bse118I RCCGGY 1 cut(s) 2475
Bse1I ACTGG 7 cut(s) 319, 970, 1459, 1610, 1750, 2216, 2747
Bse3DI GCAATG 3 cut(s) 1473, 1806, 1830
BseBI CCWGG 4 cut(s) 692, 906, 1914, 2289
BseDI CCNNGG 4 cut(s) 256, 690, 750, 1237
BseGI GGATG 6 cut(s) 160, 406, 700, 870, 2343, 2832
BseLI CCNNNNNNNGG 6 cut(s) 334, 1616, 1913, 2435, 2613, 2849
BseMI GCAATG 3 cut(s) 1473, 1806, 1830
BseMII CTCAG 9 cut(s) 204, 419, 473, 753, 969, 1210, 2089, 2517, 2547
BseNI ACTGG 7 cut(s) 319, 970, 1459, 1610, 1750, 2216, 2747
BseRI GAGGAG 4 cut(s) 1350, 1556, 1890, 2750
BseSI GKGCMC 1 cut(s) 2509
BsgI GTGCAG 1 cut(s) 1349
BshFI GGCC 9 cut(s) 107, 128, 237, 261, 381, 1458, 2292, 2617, 2853
BshNI GGYRCC 2 cut(s) 2178, 2521
BshTI ACCGGT 1 cut(s) 2475
BsiHKAI GWGCWC 3 cut(s) 1415, 2509, 2652
BsiHKCI CYCGRG 3 cut(s) 473, 1088, 1651
BsiSI CCGG 9 cut(s) 108, 122, 387, 398, 750, 1225, 2476, 2607, 2729
BslFI GGGAC 5 cut(s) 361, 1207, 1648, 2116, 2781
BslI CCNNNNNNNGG 6 cut(s) 334, 1616, 1913, 2435, 2613, 2849
BsmBI CGTCTC 1 cut(s) 22
BsmFI GGGAC 5 cut(s) 361, 1207, 1648, 2116, 2781
BsmI GAATGC 2 cut(s) 1317, 1826
BsnI GGCC 9 cut(s) 107, 128, 237, 261, 381, 1458, 2292, 2617, 2853
Bso31I GGTCTC 2 cut(s) 1099, 2306
BsoBI CYCGRG 3 cut(s) 473, 1088, 1651
Bsp1286I GDGCHC 5 cut(s) 1415, 2008, 2509, 2652, 2674
Bsp143I GATC 5 cut(s) 93, 331, 393, 2124, 2751
BspACI CCGC 4 cut(s) 506, 1876, 2298, 2614
BspANI GGCC 9 cut(s) 107, 128, 237, 261, 381, 1458, 2292, 2617, 2853
BspCNI CTCAG 9 cut(s) 205, 420, 474, 754, 970, 1209, 2088, 2518, 2548
BspHI TCATGA 1 cut(s) 616
BspLI GGNNCC 3 cut(s) 2005, 2180, 2523
BspMAI CTGCAG 1 cut(s) 1471
BspPI GGATC 1 cut(s) 388
BspT107I GGYRCC 2 cut(s) 2178, 2521
BspTNI GGTCTC 2 cut(s) 1099, 2306
BsrDI GCAATG 3 cut(s) 1473, 1806, 1830
BsrFI RCCGGY 1 cut(s) 2475
BsrI ACTGG 7 cut(s) 319, 970, 1459, 1610, 1750, 2216, 2747
BssAI RCCGGY 1 cut(s) 2475
BssECI CCNNGG 4 cut(s) 256, 690, 750, 1237
BssMI GATC 5 cut(s) 93, 331, 393, 2124, 2751
BssNI GRCGYC 3 cut(s) 1129, 1271, 2522
BssT1I CCWWGG 1 cut(s) 1237
Bst2UI CCWGG 4 cut(s) 692, 906, 1914, 2289
Bst4CI ACNGT 6 cut(s) 161, 1858, 2030, 2071, 2113, 2545
Bst6I CTCTTC 2 cut(s) 144, 1908
BstACI GRCGYC 3 cut(s) 1129, 1271, 2522
BstAPI GCANNNNNTGC 2 cut(s) 1475, 1832
BstC8I GCNNGC 5 cut(s) 219, 974, 1205, 1535, 1964
BstEII GGTNACC 2 cut(s) 36, 2602
BstENI CCTNNNNNAGG 1 cut(s) 2847
BstF5I GGATG 6 cut(s) 160, 406, 700, 870, 2343, 2832
BstH2I RGCGCY 1 cut(s) 2525
BstHHI GCGC 1 cut(s) 2524
BstKTI GATC 5 cut(s) 96, 334, 396, 2127, 2754
BstMBI GATC 5 cut(s) 93, 331, 393, 2124, 2751
BstNI CCWGG 4 cut(s) 692, 906, 1914, 2289
BstNSI RCATGY 2 cut(s) 251, 1874
BstPAI GACNNNNGTC 1 cut(s) 2445
BstPI GGTNACC 2 cut(s) 36, 2602
BstSFI CTRYAG 1 cut(s) 1467
BstSLI GKGCMC 1 cut(s) 2509
BstV2I GAAGAC 4 cut(s) 91, 1124, 1143, 1872
BstXI CCANNNNNNTGG 2 cut(s) 698, 1526
BsuI GTATCC 1 cut(s) 1591
BsuRI GGCC 9 cut(s) 107, 128, 237, 261, 381, 1458, 2292, 2617, 2853
BtgZI GCGATG 2 cut(s) 1380, 1410
BtsCI GGATG 6 cut(s) 160, 406, 700, 870, 2343, 2832
BtsIMutI CAGTG 6 cut(s) 312, 425, 739, 1617, 1743, 2754
Cac8I GCNNGC 5 cut(s) 219, 974, 1205, 1535, 1964
CciI TCATGA 1 cut(s) 616
CfoI GCGC 1 cut(s) 2524
Cfr10I RCCGGY 1 cut(s) 2475
Cfr13I GGNCC 3 cut(s) 1457, 2071, 2285
CsiI ACCWGGT 1 cut(s) 1912
Csp6I GTAC 5 cut(s) 50, 1093, 1242, 1944, 2578
CspAI ACCGGT 1 cut(s) 2475
CspCI CAANNNNNGTGG 2 cut(s) 1729, 1764
CviQI GTAC 5 cut(s) 50, 1093, 1242, 1944, 2578
DinI GGCGCC 1 cut(s) 2523
DpnI GATC 5 cut(s) 95, 333, 395, 2126, 2753
DpnII GATC 5 cut(s) 93, 331, 393, 2124, 2751
DraI TTTAAA 2 cut(s) 804, 1515
DriI GACNNNNNGTC 1 cut(s) 1773
EaeI YGGCCR 1 cut(s) 2290
Eam1104I CTCTTC 2 cut(s) 144, 1908
Eam1105I GACNNNNNGTC 1 cut(s) 1773
EarI CTCTTC 2 cut(s) 144, 1908
Eco130I CCWWGG 1 cut(s) 1237
Eco147I AGGCCT 1 cut(s) 261
Eco24I GRGCYC 2 cut(s) 2008, 2674
Eco31I GGTCTC 2 cut(s) 1099, 2306
Eco47I GGWCC 2 cut(s) 2071, 2285
Eco57I CTGAAG 3 cut(s) 1118, 1580, 2829
Eco88I CYCGRG 3 cut(s) 473, 1088, 1651
Eco91I GGTNACC 2 cut(s) 36, 2602
EcoNI CCTNNNNNAGG 1 cut(s) 2847
EcoO65I GGTNACC 2 cut(s) 36, 2602
EcoRI GAATTC 2 cut(s) 756, 900
EcoRII CCWGG 4 cut(s) 690, 904, 1912, 2287
EcoT14I CCWWGG 1 cut(s) 1237
EcoT22I ATGCAT 2 cut(s) 1162, 1872
EcoT38I GRGCYC 2 cut(s) 2008, 2674
EgeI GGCGCC 1 cut(s) 2523
EheI GGCGCC 1 cut(s) 2523
ErhI CCWWGG 1 cut(s) 1237
Esp3I CGTCTC 1 cut(s) 22
FalI AAGNNNNNCTT 6 cut(s) 569, 601, 1306, 1338, 2685, 2717
FaqI GGGAC 5 cut(s) 361, 1207, 1648, 2116, 2781
FauNDI CATATG 1 cut(s) 1279
FbaI TGATCA 2 cut(s) 2124, 2751
FblI GTMKAC 1 cut(s) 1503
FokI GGATG 6 cut(s) 167, 413, 707, 877, 2350, 2819
FriOI GRGCYC 2 cut(s) 2008, 2674
FspBI CTAG 6 cut(s) 116, 224, 650, 1007, 1013, 2675
GlaI GCGC 1 cut(s) 2523
GsuI CTGGAG 2 cut(s) 623, 2022
HaeII RGCGCY 1 cut(s) 2525
HaeIII GGCC 9 cut(s) 107, 128, 237, 261, 381, 1458, 2292, 2617, 2853
HapII CCGG 9 cut(s) 108, 122, 387, 398, 750, 1225, 2476, 2607, 2729
HhaI GCGC 1 cut(s) 2524
Hin1I GRCGYC 3 cut(s) 1129, 1271, 2522
Hin6I GCGC 1 cut(s) 2522
HinP1I GCGC 1 cut(s) 2522
HincII GTYRAC 6 cut(s) 655, 987, 1504, 1548, 2109, 2818
HindII GTYRAC 6 cut(s) 655, 987, 1504, 1548, 2109, 2818
HindIII AAGCTT 3 cut(s) 1064, 1964, 2756
HpaI GTTAAC 1 cut(s) 655
HpaII CCGG 9 cut(s) 108, 122, 387, 398, 750, 1225, 2476, 2607, 2729
Hpy99I CGWCG 4 cut(s) 80, 83, 523, 1505
HpyCH4III ACNGT 6 cut(s) 161, 1858, 2030, 2071, 2113, 2545
HpyCH4IV ACGT 4 cut(s) 81, 1129, 1271, 1500
HpySE526I ACGT 4 cut(s) 81, 1129, 1271, 1500
Hsp92I GRCGYC 3 cut(s) 1129, 1271, 2522
HspAI GCGC 1 cut(s) 2522
KasI GGCGCC 1 cut(s) 2521
Ksp22I TGATCA 2 cut(s) 2124, 2751
KspAI GTTAAC 1 cut(s) 655
Kzo9I GATC 5 cut(s) 93, 331, 393, 2124, 2751
LmnI GCTCC 7 cut(s) 969, 1239, 1337, 1354, 1530, 1903, 2003
LweI GCATC 4 cut(s) 1975, 2371, 2490, 2783
MabI ACCWGGT 1 cut(s) 1912
MaeI CTAG 6 cut(s) 116, 224, 650, 1007, 1013, 2675
MaeII ACGT 4 cut(s) 81, 1129, 1271, 1500
MalI GATC 5 cut(s) 95, 333, 395, 2126, 2753
MboI GATC 5 cut(s) 93, 331, 393, 2124, 2751
MfeI CAATTG 3 cut(s) 169, 1300, 1836
MhlI GDGCHC 5 cut(s) 1415, 2008, 2509, 2652, 2674
MlsI TGGCCA 1 cut(s) 2292
MluNI TGGCCA 1 cut(s) 2292
Mly113I GGCGCC 1 cut(s) 2522
MlyI GAGTC 7 cut(s) 216, 282, 485, 773, 989, 1762, 2209
MmeI TCCRAC 1 cut(s) 679
Mox20I TGGCCA 1 cut(s) 2292
Mph1103I ATGCAT 2 cut(s) 1162, 1872
MscI TGGCCA 1 cut(s) 2292
MseI TTAA 7 cut(s) 59, 654, 771, 803, 1514, 1683, 2157
MslI CAYNNNNRTG 1 cut(s) 1782
Msp20I TGGCCA 1 cut(s) 2292
MspA1I CMGCKG 1 cut(s) 2230
MspI CCGG 9 cut(s) 108, 122, 387, 398, 750, 1225, 2476, 2607, 2729
MunI CAATTG 3 cut(s) 169, 1300, 1836
Mva1269I GAATGC 2 cut(s) 1317, 1826
MvaI CCWGG 4 cut(s) 692, 906, 1914, 2289
NarI GGCGCC 1 cut(s) 2522
NciI CCSGG 6 cut(s) 109, 122, 388, 398, 751, 1225
NdeI CATATG 1 cut(s) 1279
NdeII GATC 5 cut(s) 93, 331, 393, 2124, 2751
NlaIV GGNNCC 3 cut(s) 2005, 2180, 2523
NmuCI GTSAC 7 cut(s) 3, 36, 423, 1775, 2191, 2602, 2654
NsiI ATGCAT 2 cut(s) 1162, 1872
NspI RCATGY 2 cut(s) 251, 1874
PaeR7I CTCGAG 1 cut(s) 1088
PagI TCATGA 1 cut(s) 616
PceI AGGCCT 1 cut(s) 261
PcsI WCGNNNNNNNCGW 1 cut(s) 1658
PctI GAATGC 2 cut(s) 1317, 1826
PfeI GAWTC 6 cut(s) 468, 523, 1228, 1949, 2272, 2377
PflMI CCANNNNNTGG 1 cut(s) 1913
PfoI TCCNGGA 2 cut(s) 396, 1223
PinAI ACCGGT 1 cut(s) 2475
PleI GAGTC 7 cut(s) 215, 282, 484, 772, 988, 1762, 2209
PluTI GGCGCC 1 cut(s) 2525
PpsI GAGTC 7 cut(s) 215, 282, 484, 772, 988, 1762, 2209
PshAI GACNNNNGTC 1 cut(s) 2445
Psp6I CCWGG 4 cut(s) 690, 904, 1912, 2287
PspEI GGTNACC 2 cut(s) 36, 2602
PspGI CCWGG 4 cut(s) 690, 904, 1912, 2287
PspN4I GGNNCC 3 cut(s) 2005, 2180, 2523
PspPI GGNCC 3 cut(s) 1457, 2071, 2285
PspXI VCTCGAGB 1 cut(s) 1088
PstI CTGCAG 1 cut(s) 1471
PvuII CAGCTG 1 cut(s) 2230
RsaI GTAC 5 cut(s) 51, 1094, 1243, 1945, 2579
RsaNI GTAC 5 cut(s) 50, 1093, 1242, 1944, 2578
RseI CAYNNNNRTG 1 cut(s) 1782
SalI GTCGAC 1 cut(s) 1502
SaqAI TTAA 7 cut(s) 59, 654, 771, 803, 1514, 1683, 2157
Sau3AI GATC 5 cut(s) 93, 331, 393, 2124, 2751
Sau96I GGNCC 3 cut(s) 1457, 2071, 2285
ScaI AGTACT 1 cut(s) 1945
SchI GAGTC 7 cut(s) 216, 282, 485, 773, 989, 1762, 2209
SduI GDGCHC 5 cut(s) 1415, 2008, 2509, 2652, 2674
SexAI ACCWGGT 1 cut(s) 1912
SfaNI GCATC 4 cut(s) 1975, 2371, 2490, 2783
SfcI CTRYAG 1 cut(s) 1467
SfoI GGCGCC 1 cut(s) 2523
Sfr274I CTCGAG 1 cut(s) 1088
SinI GGWCC 2 cut(s) 2071, 2285
SlaI CTCGAG 1 cut(s) 1088
SmiMI CAYNNNNRTG 1 cut(s) 1782
SmlI CTYRAG 6 cut(s) 932, 1088, 1291, 1349, 2116, 2485
SmoI CTYRAG 6 cut(s) 932, 1088, 1291, 1349, 2116, 2485
SpeI ACTAGT 1 cut(s) 649
SseBI AGGCCT 1 cut(s) 261
SsiI CCGC 4 cut(s) 506, 1876, 2298, 2614
SspDI GGCGCC 1 cut(s) 2521
SspI AATATT 1 cut(s) 2404
SspMI CTAG 6 cut(s) 116, 224, 650, 1007, 1013, 2675
StuI AGGCCT 1 cut(s) 261
StyI CCWWGG 1 cut(s) 1237
TaaI ACNGT 6 cut(s) 161, 1858, 2030, 2071, 2113, 2545
TaiI ACGT 4 cut(s) 84, 1132, 1274, 1503
TaqI TCGA 7 cut(s) 392, 521, 767, 1089, 1503, 2145, 2365
TatI WGTACW 2 cut(s) 1092, 1943
TauI GCSGC 1 cut(s) 2617
TfiI GAWTC 6 cut(s) 468, 523, 1228, 1949, 2272, 2377
Tru1I TTAA 7 cut(s) 59, 654, 771, 803, 1514, 1683, 2157
Tru9I TTAA 7 cut(s) 59, 654, 771, 803, 1514, 1683, 2157
TscAI CASTG 6 cut(s) 319, 432, 739, 1617, 1750, 2754
TseFI GTSAC 7 cut(s) 3, 36, 423, 1775, 2191, 2602, 2654
Tsp45I GTSAC 7 cut(s) 3, 36, 423, 1775, 2191, 2602, 2654
TspGWI ACGGA 1 cut(s) 1650
TspRI CASTG 6 cut(s) 319, 432, 739, 1617, 1750, 2754
Van91I CCANNNNNTGG 1 cut(s) 1913
VneI GTGCAC 1 cut(s) 2505
VpaK11BI GGWCC 2 cut(s) 2071, 2285
XagI CCTNNNNNAGG 1 cut(s) 2847
XapI RAATTY 4 cut(s) 756, 900, 943, 1732
XbaI TCTAGA 3 cut(s) 223, 1012, 2674
XceI RCATGY 2 cut(s) 251, 1874
XcmI CCANNNNNNNNNTGG 2 cut(s) 552, 2507
XhoI CTCGAG 1 cut(s) 1088
XmiI GTMKAC 1 cut(s) 1503
XspI CTAG 6 cut(s) 116, 224, 650, 1007, 1013, 2675
ZraI GACGTC 2 cut(s) 1130, 1272
ZrmI AGTACT 1 cut(s) 1945
Zsp2I ATGCAT 2 cut(s) 1162, 1872
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.