Rw5G039520

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000085
Physical Location & Seq
Forward (+)
69707896 .. 69713208
5313 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G039520.1

Sequence Viewer

Length: 3354 bp
ATGGTGAAACATGTCGGCGCGCAAGCCCGAGAACTTAGGCTTGTTCATTATGTGATTAGGGCTGTTGTAGCCCAGAGCCCTGATTTGAGAAATATTCAAAGCACATTGGCCAATCAGCTGGGCTTAAAATTCGAGGAGGAGGAGACAGAAATTGGAAGAGCCGCTAGATTGAAGGAGAGGATAATGGGAGGAAATAAAATCCTCATAGTCTTAGTCGACATTTGGCGGAGAATAGATTTGTCAAGCATAGGAATTCCCAATTACAACGAGCTTCAAAGACACAATTCCAAACTCATACTAACCTCAAGGAGCAGAAGTGTTTGCTATTCCATGCAGTGCCAAGCAAGCATTCCTCTCCCTATCTTATCAGAAGAAGATTCTTGGAACCTTTTTGTGGAGAAATCGGGAAAGTCTTTTCATGAATCTACCGAGCTGCATGATGTAGCAAGGACCGTAGCTAGAGAATGTGGTGGTCTCCCACTTGCACTGATAACAGTTGCTGGGGCACTTGGAGATAAAGACATAGAAGACTGGAAAGAAGCAGCTCAACGACTAGACAGGTCGCAAACAGTCAATCTGGAGGACGAGGGAGATGTATACAAATGCATAAAGTTCAGCTATGATTACTTGAGATCTGATGATGCCAAATCATTCTTCTTGCTATGCTGTCTATTCCCAGAAGACTATGATATCCCATTCGAAGACTTGCTCAAGTATGCAATCGGGAAAGGGTTGCTTCAAGATGCCAACACAATCCAAGAAGCCAGAGCAACAGCAAATTTGGTGATCAATTCCCTCAAAGCTTTCAGCCTACTTTTGGAAGGTACAAAGGAGGGATGTGTAAGGATGCATGATGTAGTGCGGGATGTAGCCCTGTCGATTTCGTTTTCTGAAGATGGTCATAAATTTTTGGTAAAAGCTGGTTGTGAATTACAGCATTGGCCAAGGATTAAAGCGCATGGAGGCTATTCTGCAATCTCACTAATGAGGAACAAAATTTGCAAGCTTCCCAAAGAGTTGGTAAGCTCAAAACTCCAGATTTTATTACTACAAAATAACGCTGCTATATGTGCGATCCCAAACAACTTTTTTCAAAGTATGAATGGACTGATGGTCCTAGATCTTAGCTTCACCAGTATCTCATGTCTACCCCGATCCTTCACTCACCTAACCAATCTCCGAGGTTTGTATTTGGATTATTGCAACAAGATGATTGACATATCCATATTCTTAAACCTGAAGAAACTTGAGATTCTTAGTATGAAAGAATTTCCTCTTGAGGAGTTGCCATTAGAAATAGGAGAGTTGACCAATCTAAGGATGCTCGACTTAAGCGGGAGTTACAATATTGGTAGCATTCCATGTAATATAATGTCAAGATTGTATAAATTGGAAGAACTGTACATGCAAGGTAATTTTGGGGACTGGGGAGGAGGACGATACAAAACTAATGCTGGTTTTGATGAGTTGATTGGCTTGCAATATTTAAACATTGTGAAGCTTCGCATATCTGATGCACAATGCTTGCCTAAAAATGTTGTATTCAATTTCAATTGGTTAATTTTCGATATATACTACTCTTCAAAACCTTATTGCTCACACGATCTTTCAAGAACCTTGACTCTTAGCACAACAATCAACACCTTACCAGATTGGTTTATCAAAGTGGTAGCAGAGAAAGCAGAGAAACTACAGTACCTAAAGTGCAAAGGCCTAAAGAACATTCTTGTGGAACATGACAGAGGGAGGTTACATGGACTGAAGTATCTAGCTGTAATTGGGCCCCATGAGTACTTGGCAGTGTTGATGGAAACAATAACACGGGTGACAGATGTATCTGTGTTCAAGAACTTGGAAGAGTTGCATCTCCTTGGTGTGAGTTTTCTGAAGGAGTTATGTGTTGGTGAATTACCGCTTGGGTCTCTATTCAATCTCAAGTCATTAAAGGTGGAATATTGCCATGACTTGGTGAATGCACTTTTGTCATCAAATTTGTTGCAAAGACTACAAAATCTGGAAAAACTTCATTGTGAAGATATGAATGTTATGGAGTATGTGTTTGGATTCGAAGGGCTGGAGCCAGAAGACATTATCTTGCCAAAATTGAGGGAGATGATATTGTGGGATCTAGAAAAACTAATAAGCATATGGAGTGGTCCTGCTCCAAATGCAGTCTTCCATAATCTTAGAAGTTTGGCAGTCTCAGGTTGCAAAAAACTTGAAAGTCTCTTTACATATGAGGTAGCAAAATGTCTTTTGCAATTGGAAGACCTTTCGGTAACTCAATGTCCTAGCTTGGACATGGTTATTAAATCAAGCAAGGAAGTAGTGGACAAGAAGATCATTTTTCCAGAATTGAAGAACTTAGCTTTGTCGTATCTTCCACGGATCACTAGGTTCTGCGGTAGTGGAATTGCTACCATGGAGTGCCCTTCATTGGAACATTTGCATGTGCAGGACTGCCCCCATTTTACAACCTCTACTTCTGACTTCCACTGCTTGAAGCAATTTCAAGTCAATGATCTACGACATTTGAGAATGAGCAAAGACTTGGATAACATCAATTTAGCTGGCCAGCATACCAGGGACATCCTTGAGAGCCTACCTCAGAATGTCAGAAAGCGGGTTGAGGCCATTAGAGAAATTGAGAACCAACATCATGAATTAGAGGCAGAGTTTCTCAAGGAGAGAGCTTTACTTGAAGCTAAGTATCATAAATTATATCAACCTCTCTACAGCAAGCGATACTTGATTGTGAATGGTGTTGTTGAAACTGAAGAAGTTGCCACTGAAGCAGCAGCAGATCAAGAGGACAAAACTGCAAAAGAGGGAGGAGTGCCTGATTTCTGGCTCAATGCATTGAAGAACAATGAATTGCTAGCCAAGGAGATCACTGAGCATGATGAAGGAGCTCTTAAATATCTGAGAGACATCAAGTGGTTTAAGATCCATAATTCCAAGGGGTTCAAACTTCAGTTCTACTTTGACACCAGTCCATTTTTCAAAAACTCTGTTTTGACAAAGACATATCACATGATTGATGAAGATGAACCAAATAACGTAGCAGGGACGGAGATTGAATGGTATCCAGCAAAATGCCTGACACAGAAGATTCTTAAGAAGAAGCGTAAGAAGGGATCGAAGAATTCTAAGCCTCTGACTAAAACTGAAAATTGTAAAAGTTTCTTCAATTTCTTTAGTCCACCTCAAGTCTCCAAGGACGATGAAATTATTGATGAATTTGCTGCCGAGGAACTCAAAAACCAGAAGAAACAGGACTATGATGTCGGGTCAACCATTCGTCATAAGATCATTCCACATGCAGTTTCGTGGTTTACTGGAGAAGCTTTTTAG

Protein Analysis

1117

Amino Acids

127.49

Weight (kDa)

6.28

Isoelectric Point (pI)

41.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 18 - 136 1.5e-13 NB-ARC domain
LRR_8 PF13855 344 - 402 1.7e-10 Leucine rich repeat
LRR_14 PF23598 397 - 501 1.4e-06 Leucine-rich repeat region
LRR_RPS2 PF23247 618 - 763 2.5e-31 Plant disease resistance protein RPS2-like, leucine-rich repeats
NAP PF00956 874 - 1115 1.4e-76 Nucleosome assembly protein (NAP)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 3284
AccB7I CCANNNNNTGG 1 cut(s) 1966
AccI GTMKAC 3 cut(s) 216, 597, 1147
AccII CGCG 1 cut(s) 20
AciI CCGC 7 cut(s) 162, 226, 862, 1335, 1913, 2403, 2623
AclWI GGATC 6 cut(s) 1069, 1149, 2133, 2396, 2941, 3145
AcoI YGGCCR 3 cut(s) 108, 941, 2572
AcsI RAATTY 9 cut(s) 128, 252, 778, 905, 996, 1268, 1990, 3145, 3239
AcuI CTGAAG 7 cut(s) 912, 1259, 1781, 1907, 2796, 2811, 2957
AfaI GTAC 4 cut(s) 826, 1403, 1697, 1793
AfiI CCNNNNNNNGG 5 cut(s) 394, 817, 1317, 1966, 2437
AflII CTTAAG 2 cut(s) 1330, 3116
AflIII ACRYGT 1 cut(s) 10
AhdI GACNNNNNGTC 1 cut(s) 1112
AjnI CCWGG 1 cut(s) 2582
AjuI GAANNNNNNNTTGG 6 cut(s) 333, 365, 638, 670, 1899, 1931
Alw21I GWGCWC 1 cut(s) 2914
Alw26I GTCTC 7 cut(s) 137, 479, 1926, 2206, 2231, 2922, 3217
AlwI GGATC 6 cut(s) 1069, 1149, 2133, 2396, 2941, 3145
AlwNI CAGNNNCTG 1 cut(s) 500
Ama87I CYCGRG 1 cut(s) 27
AoxI GGCC 6 cut(s) 108, 941, 1711, 1781, 2572, 2631
ApaI GGGCCC 1 cut(s) 1785
ApeKI GCWGC 6 cut(s) 433, 542, 1061, 2795, 2798, 3245
ApoI RAATTY 9 cut(s) 128, 252, 778, 905, 996, 1268, 1990, 3145, 3239
Asp700I GAANNNNTTC 1 cut(s) 2022
AspLEI GCGC 3 cut(s) 20, 22, 958
AspS9I GGNCC 5 cut(s) 450, 1114, 1781, 1782, 2156
AsuHPI GGTGA 7 cut(s) 16, 796, 1123, 1157, 1837, 1916, 1981
AsuII TTCGAA 2 cut(s) 699, 2067
AsuNHI GCTAGC 1 cut(s) 2878
AvaI CYCGRG 1 cut(s) 27
AvaII GGWCC 3 cut(s) 450, 1114, 2156
BaeGI GKGCMC 3 cut(s) 508, 1785, 2432
BaeI ACNNNNGTAYC 6 cut(s) 1679, 1712, 1748, 1781, 1818, 1851
BalI TGGCCA 3 cut(s) 110, 943, 2574
BanII GRGCYC 3 cut(s) 80, 1785, 2914
BbsI GAAGAC 6 cut(s) 534, 687, 708, 2091, 2167, 2274
Bbv12I GWGCWC 1 cut(s) 2914
BbvI GCAGC 6 cut(s) 420, 554, 1048, 2807, 2810, 3232
BccI CCATC 3 cut(s) 890, 1105, 1801
BciT130I CCWGG 1 cut(s) 2584
BciVI GTATCC 1 cut(s) 3096
BclI TGATCA 1 cut(s) 786
BcoDI GTCTC 7 cut(s) 137, 479, 1926, 2206, 2231, 2922, 3217
BfaI CTAG 9 cut(s) 165, 459, 554, 1118, 1769, 2129, 2292, 2394, 2879
BfmI CTRYAG 2 cut(s) 1691, 2734
BfrI CTTAAG 2 cut(s) 1330, 3116
BfuI GTATCC 1 cut(s) 3096
BglII AGATCT 2 cut(s) 632, 1120
BisI GCNGC 7 cut(s) 162, 434, 543, 1062, 2796, 2799, 3246
BlsI GCNGC 7 cut(s) 163, 435, 544, 1063, 2797, 2800, 3247
BmcAI AGTACT 1 cut(s) 1793
Bme1390I CCNGG 1 cut(s) 2584
Bme18I GGWCC 3 cut(s) 450, 1114, 2156
BmeRI GACNNNNNGTC 1 cut(s) 1112
BmeT110I CYCGRG 1 cut(s) 27
BmgT120I GGNCC 5 cut(s) 450, 1114, 1781, 1782, 2156
BmiI GGNNCC 4 cut(s) 386, 1783, 1784, 2079
BmrFI CCNGG 1 cut(s) 2584
BmrI ACTGGG 1 cut(s) 1435
BmsI GCATC 6 cut(s) 631, 733, 837, 1311, 1504, 1873
BmtI GCTAGC 1 cut(s) 2882
BmuI ACTGGG 1 cut(s) 1435
BoxI GACNNNNGTC 1 cut(s) 2991
BpiI GAAGAC 6 cut(s) 534, 687, 708, 2091, 2167, 2274
BplI GAGNNNNNCTC 2 cut(s) 2590, 2622
BpmI CTGGAG 3 cut(s) 599, 1019, 2096
Bpu14I TTCGAA 2 cut(s) 699, 2067
BpuEI CTTGAG 9 cut(s) 289, 649, 695, 1268, 1298, 1919, 2615, 2666, 3192
BsaI GGTCTC 2 cut(s) 479, 1926
BsaXI ACNNNNNCTCC 4 cut(s) 301, 331, 2042, 2072
Bsc4I CCNNNNNNNGG 5 cut(s) 394, 817, 1317, 1966, 2437
Bse1I ACTGG 5 cut(s) 536, 1134, 1430, 2991, 3343
BseBI CCWGG 1 cut(s) 2584
BseGI GGATG 5 cut(s) 842, 852, 871, 1326, 2589
BseLI CCNNNNNNNGG 5 cut(s) 394, 817, 1317, 1966, 2437
BseMII CTCAG 4 cut(s) 2217, 2621, 2886, 2915
BseNI ACTGG 5 cut(s) 536, 1134, 1430, 2991, 3343
BsePI GCGCGC 1 cut(s) 18
BseRI GAGGAG 6 cut(s) 149, 152, 155, 1295, 1446, 2847
BseSI GKGCMC 3 cut(s) 508, 1785, 2432
BseXI GCAGC 6 cut(s) 420, 554, 1048, 2807, 2810, 3232
BseYI CCCAGC 2 cut(s) 118, 500
BsgI GTGCAG 1 cut(s) 2474
Bsh1236I CGCG 1 cut(s) 20
BshFI GGCC 6 cut(s) 110, 943, 1713, 1783, 2574, 2633
BsiHKAI GWGCWC 1 cut(s) 2914
BsiHKCI CYCGRG 1 cut(s) 27
BslFI GGGAC 3 cut(s) 1436, 2600, 3082
BslI CCNNNNNNNGG 5 cut(s) 394, 817, 1317, 1966, 2437
BsmAI GTCTC 7 cut(s) 137, 479, 1926, 2206, 2231, 2922, 3217
BsmFI GGGAC 3 cut(s) 1436, 2600, 3082
BsmI GAATGC 3 cut(s) 348, 1356, 1978
BsnI GGCC 6 cut(s) 110, 943, 1713, 1783, 2574, 2633
Bso31I GGTCTC 2 cut(s) 479, 1926
BsoBI CYCGRG 1 cut(s) 27
Bsp119I TTCGAA 2 cut(s) 699, 2067
Bsp120I GGGCCC 1 cut(s) 1781
Bsp1286I GDGCHC 5 cut(s) 80, 508, 1785, 2432, 2914
Bsp1407I TGTACA 1 cut(s) 1401
Bsp19I CCATGG 1 cut(s) 2421
BspACI CCGC 7 cut(s) 162, 226, 862, 1335, 1913, 2403, 2623
BspANI GGCC 6 cut(s) 110, 943, 1713, 1783, 2574, 2633
BspCNI CTCAG 4 cut(s) 2216, 2620, 2887, 2916
BspFNI CGCG 1 cut(s) 20
BspHI TCATGA 2 cut(s) 418, 2659
BspLI GGNNCC 4 cut(s) 386, 1783, 1784, 2079
BspOI GCTAGC 1 cut(s) 2882
BspPI GGATC 6 cut(s) 1069, 1149, 2133, 2396, 2941, 3145
BspQI GCTCTTC 1 cut(s) 151
BspT104I TTCGAA 2 cut(s) 699, 2067
BspTI CTTAAG 2 cut(s) 1330, 3116
BspTNI GGTCTC 2 cut(s) 479, 1926
BsrGI TGTACA 1 cut(s) 1401
BsrI ACTGG 5 cut(s) 536, 1134, 1430, 2991, 3343
BssHII GCGCGC 1 cut(s) 18
BssNAI GTATAC 1 cut(s) 598
BssT1I CCWWGG 6 cut(s) 944, 1870, 2421, 2883, 2958, 3216
Bst1107I GTATAC 1 cut(s) 598
Bst2UI CCWGG 1 cut(s) 2584
Bst4CI ACNGT 5 cut(s) 454, 496, 571, 1401, 1695
Bst6I CTCTTC 3 cut(s) 151, 1585, 1851
BstAFI CTTAAG 2 cut(s) 1330, 3116
BstAUI TGTACA 1 cut(s) 1401
BstBI TTCGAA 2 cut(s) 699, 2067
BstDSI CCRYGG 2 cut(s) 2384, 2421
BstF5I GGATG 5 cut(s) 842, 852, 871, 1326, 2589
BstFNI CGCG 1 cut(s) 20
BstHHI GCGC 3 cut(s) 20, 22, 958
BstMAI GTCTC 7 cut(s) 137, 479, 1926, 2206, 2231, 2922, 3217
BstMWI GCNNNNNNNGC 6 cut(s) 68, 345, 1070, 1679, 2168, 2792
BstNI CCWGG 1 cut(s) 2584
BstNSI RCATGY 4 cut(s) 14, 1408, 2453, 3323
BstPAI GACNNNNGTC 1 cut(s) 2991
BstSCI CCNGG 1 cut(s) 2582
BstSFI CTRYAG 2 cut(s) 1691, 2734
BstSLI GKGCMC 3 cut(s) 508, 1785, 2432
BstUI CGCG 1 cut(s) 20
BstV1I GCAGC 6 cut(s) 420, 554, 1048, 2807, 2810, 3232
BstV2I GAAGAC 6 cut(s) 534, 687, 708, 2091, 2167, 2274
BstX2I RGATCY 4 cut(s) 632, 1120, 2125, 2946
BstXI CCANNNNNNTGG 2 cut(s) 118, 1018
BstYI RGATCY 4 cut(s) 632, 1120, 2125, 2946
BstZ17I GTATAC 1 cut(s) 598
BsuI GTATCC 1 cut(s) 3096
BsuRI GGCC 6 cut(s) 110, 943, 1713, 1783, 2574, 2633
BtgI CCRYGG 2 cut(s) 2384, 2421
BtsCI GGATG 5 cut(s) 842, 852, 871, 1326, 2589
BtsI GCAGTG 3 cut(s) 341, 1806, 2494
BtsIMutI CAGTG 6 cut(s) 341, 485, 1806, 2494, 2787, 2892
CaiI CAGNNNCTG 1 cut(s) 500
CciI TCATGA 2 cut(s) 418, 2659
CfoI GCGC 3 cut(s) 20, 22, 958
Cfr13I GGNCC 5 cut(s) 450, 1114, 1781, 1782, 2156
Csp6I GTAC 4 cut(s) 825, 1402, 1696, 1792
CviQI GTAC 4 cut(s) 825, 1402, 1696, 1792
DraI TTTAAA 1 cut(s) 1488
DrdI GACNNNNNNGTC 1 cut(s) 3284
DriI GACNNNNNGTC 1 cut(s) 1112
DseDI GACNNNNNNGTC 1 cut(s) 3284
EaeI YGGCCR 3 cut(s) 108, 941, 2572
Eam1104I CTCTTC 3 cut(s) 151, 1585, 1851
Eam1105I GACNNNNNGTC 1 cut(s) 1112
EarI CTCTTC 3 cut(s) 151, 1585, 1851
EciI GGCGGA 1 cut(s) 241
Ecl136II GAGCTC 1 cut(s) 2912
Eco130I CCWWGG 6 cut(s) 944, 1870, 2421, 2883, 2958, 3216
Eco147I AGGCCT 1 cut(s) 1713
Eco24I GRGCYC 3 cut(s) 80, 1785, 2914
Eco31I GGTCTC 2 cut(s) 479, 1926
Eco32I GATATC 1 cut(s) 691
Eco47I GGWCC 3 cut(s) 450, 1114, 2156
Eco53kI GAGCTC 1 cut(s) 2912
Eco57I CTGAAG 7 cut(s) 912, 1259, 1781, 1907, 2796, 2811, 2957
Eco88I CYCGRG 1 cut(s) 27
EcoICRI GAGCTC 1 cut(s) 2912
EcoO109I RGGNCCY 1 cut(s) 1782
EcoRI GAATTC 2 cut(s) 252, 3145
EcoRII CCWGG 1 cut(s) 2582
EcoRV GATATC 1 cut(s) 691
EcoT14I CCWWGG 6 cut(s) 944, 1870, 2421, 2883, 2958, 3216
EcoT22I ATGCAT 3 cut(s) 608, 852, 2860
EcoT38I GRGCYC 3 cut(s) 80, 1785, 2914
ErhI CCWWGG 6 cut(s) 944, 1870, 2421, 2883, 2958, 3216
FalI AAGNNNNNCTT 6 cut(s) 720, 752, 2732, 2764, 2898, 2930
FaqI GGGAC 3 cut(s) 1436, 2600, 3082
FauI CCCGC 3 cut(s) 855, 1328, 2616
FauNDI CATATG 2 cut(s) 2147, 2236
FbaI TGATCA 1 cut(s) 786
FblI GTMKAC 3 cut(s) 216, 597, 1147
Fnu4HI GCNGC 7 cut(s) 162, 434, 543, 1062, 2796, 2799, 3246
FokI GGATG 5 cut(s) 849, 859, 878, 1333, 2576
FriOI GRGCYC 3 cut(s) 80, 1785, 2914
Fsp4HI GCNGC 7 cut(s) 162, 434, 543, 1062, 2796, 2799, 3246
FspBI CTAG 9 cut(s) 165, 459, 554, 1118, 1769, 2129, 2292, 2394, 2879
GlaI GCGC 3 cut(s) 19, 21, 957
GluI GCNGC 7 cut(s) 162, 434, 543, 1062, 2796, 2799, 3246
GsaI CCCAGC 2 cut(s) 122, 504
GsuI CTGGAG 3 cut(s) 599, 1019, 2096
HaeIII GGCC 6 cut(s) 110, 943, 1713, 1783, 2574, 2633
HhaI GCGC 3 cut(s) 20, 22, 958
Hin6I GCGC 3 cut(s) 18, 20, 956
HinP1I GCGC 3 cut(s) 18, 20, 956
HincII GTYRAC 3 cut(s) 217, 1308, 3294
HindII GTYRAC 3 cut(s) 217, 1308, 3294
HindIII AAGCTT 4 cut(s) 801, 1004, 1499, 3345
HinfI GANTC 6 cut(s) 377, 422, 1252, 1621, 2064, 3112
HphI GGTGA 7 cut(s) 16, 796, 1123, 1157, 1837, 1916, 1981
Hpy166II GTNNAC 8 cut(s) 217, 598, 1148, 1308, 2332, 3203, 3294, 3336
Hpy8I GTNNAC 8 cut(s) 217, 598, 1148, 1308, 2332, 3203, 3294, 3336
HpyAV CCTTC 8 cut(s) 166, 815, 1168, 1882, 2063, 2442, 2900, 3127
HpyCH4III ACNGT 5 cut(s) 454, 496, 571, 1401, 1695
HpyCH4IV ACGT 1 cut(s) 3060
HpyF10VI GCNNNNNNNGC 6 cut(s) 68, 345, 1070, 1679, 2168, 2792
HpySE526I ACGT 1 cut(s) 3060
HspAI GCGC 3 cut(s) 18, 20, 956
Ksp22I TGATCA 1 cut(s) 786
LguI GCTCTTC 1 cut(s) 151
LmnI GCTCC 4 cut(s) 309, 2077, 2167, 2909
Lsp1109I GCAGC 6 cut(s) 420, 554, 1048, 2807, 2810, 3232
LweI GCATC 6 cut(s) 631, 733, 837, 1311, 1504, 1873
MaeI CTAG 9 cut(s) 165, 459, 554, 1118, 1769, 2129, 2292, 2394, 2879
MaeII ACGT 1 cut(s) 3060
MaeIII GTNAC 4 cut(s) 1340, 1749, 1825, 2278
MfeI CAATTG 2 cut(s) 1552, 2261
MflI RGATCY 4 cut(s) 632, 1120, 2125, 2946
MhlI GDGCHC 5 cut(s) 80, 508, 1785, 2432, 2914
MlsI TGGCCA 3 cut(s) 110, 943, 2574
MluNI TGGCCA 3 cut(s) 110, 943, 2574
MlyI GAGTC 1 cut(s) 1615
Mox20I TGGCCA 3 cut(s) 110, 943, 2574
Mph1103I ATGCAT 3 cut(s) 608, 852, 2860
MroXI GAANNNNTTC 1 cut(s) 2022
MscI TGGCCA 3 cut(s) 110, 943, 2574
MslI CAYNNNNRTG 2 cut(s) 1727, 2448
Msp20I TGGCCA 3 cut(s) 110, 943, 2574
MspA1I CMGCKG 1 cut(s) 118
MspCI CTTAAG 2 cut(s) 1330, 3116
MspR9I CCNGG 1 cut(s) 2584
MunI CAATTG 2 cut(s) 1552, 2261
Mva1269I GAATGC 3 cut(s) 348, 1356, 1978
MvaI CCWGG 1 cut(s) 2584
MvnI CGCG 1 cut(s) 20
MwoI GCNNNNNNNGC 6 cut(s) 68, 345, 1070, 1679, 2168, 2792
NcoI CCATGG 1 cut(s) 2421
NdeI CATATG 2 cut(s) 2147, 2236
NheI GCTAGC 1 cut(s) 2878
NlaIV GGNNCC 4 cut(s) 386, 1783, 1784, 2079
NmeAIII GCCGAG 1 cut(s) 3274
NmuCI GTSAC 1 cut(s) 1825
NsiI ATGCAT 3 cut(s) 608, 852, 2860
NspI RCATGY 4 cut(s) 14, 1408, 2453, 3323
NspV TTCGAA 2 cut(s) 699, 2067
PagI TCATGA 2 cut(s) 418, 2659
PauI GCGCGC 1 cut(s) 18
PceI AGGCCT 1 cut(s) 1713
PciI ACATGT 1 cut(s) 10
PciSI GCTCTTC 1 cut(s) 151
PctI GAATGC 3 cut(s) 348, 1356, 1978
PdmI GAANNNNTTC 1 cut(s) 2022
PfeI GAWTC 5 cut(s) 377, 422, 1252, 2064, 3112
PflMI CCANNNNNTGG 1 cut(s) 1966
PkrI GCNGC 7 cut(s) 163, 435, 544, 1063, 2797, 2800, 3247
PleI GAGTC 1 cut(s) 1615
PpsI GAGTC 1 cut(s) 1615
PscI ACATGT 1 cut(s) 10
PshAI GACNNNNGTC 1 cut(s) 2991
Psp124BI GAGCTC 1 cut(s) 2914
Psp6I CCWGG 1 cut(s) 2582
PspFI CCCAGC 2 cut(s) 118, 500
PspGI CCWGG 1 cut(s) 2582
PspN4I GGNNCC 4 cut(s) 386, 1783, 1784, 2079
PspOMI GGGCCC 1 cut(s) 1781
PspPI GGNCC 5 cut(s) 450, 1114, 1781, 1782, 2156
PstNI CAGNNNCTG 1 cut(s) 500
PsuI RGATCY 4 cut(s) 632, 1120, 2125, 2946
PteI GCGCGC 1 cut(s) 18
PvuII CAGCTG 1 cut(s) 118
RsaI GTAC 4 cut(s) 826, 1403, 1697, 1793
RsaNI GTAC 4 cut(s) 825, 1402, 1696, 1792
RseI CAYNNNNRTG 2 cut(s) 1727, 2448
SacI GAGCTC 1 cut(s) 2914
SalI GTCGAC 1 cut(s) 215
SapI GCTCTTC 1 cut(s) 151
SatI GCNGC 7 cut(s) 162, 434, 543, 1062, 2796, 2799, 3246
Sau96I GGNCC 5 cut(s) 450, 1114, 1781, 1782, 2156
ScaI AGTACT 1 cut(s) 1793
SchI GAGTC 1 cut(s) 1615
ScrFI CCNGG 1 cut(s) 2584
SduI GDGCHC 5 cut(s) 80, 508, 1785, 2432, 2914
SfaNI GCATC 6 cut(s) 631, 733, 837, 1311, 1504, 1873
SfcI CTRYAG 2 cut(s) 1691, 2734
SfuI TTCGAA 2 cut(s) 699, 2067
SinI GGWCC 3 cut(s) 450, 1114, 2156
SmiMI CAYNNNNRTG 2 cut(s) 1727, 2448
SseBI AGGCCT 1 cut(s) 1713
SsiI CCGC 7 cut(s) 162, 226, 862, 1335, 1913, 2403, 2623
SspI AATATT 4 cut(s) 94, 1348, 1484, 1955
SspMI CTAG 9 cut(s) 165, 459, 554, 1118, 1769, 2129, 2292, 2394, 2879
SstI GAGCTC 1 cut(s) 2914
StuI AGGCCT 1 cut(s) 1713
StyD4I CCNGG 1 cut(s) 2582
StyI CCWWGG 6 cut(s) 944, 1870, 2421, 2883, 2958, 3216
TaaI ACNGT 5 cut(s) 454, 496, 571, 1401, 1695
TaiI ACGT 1 cut(s) 3063
TaqI TCGA 8 cut(s) 132, 216, 699, 878, 1326, 1566, 2067, 3140
TatI WGTACW 2 cut(s) 1401, 1791
TauI GCSGC 1 cut(s) 164
TfiI GAWTC 5 cut(s) 377, 422, 1252, 2064, 3112
TscAI CASTG 6 cut(s) 341, 492, 1806, 2501, 2794, 2899
TseFI GTSAC 1 cut(s) 1825
TseI GCWGC 6 cut(s) 433, 542, 1061, 2795, 2798, 3245
Tsp45I GTSAC 1 cut(s) 1825
TspGWI ACGGA 2 cut(s) 2401, 3086
TspRI CASTG 6 cut(s) 341, 492, 1806, 2501, 2794, 2899
Van91I CCANNNNNTGG 1 cut(s) 1966
Vha464I CTTAAG 2 cut(s) 1330, 3116
VpaK11BI GGWCC 3 cut(s) 450, 1114, 2156
XapI RAATTY 9 cut(s) 128, 252, 778, 905, 996, 1268, 1990, 3145, 3239
XbaI TCTAGA 1 cut(s) 2128
XceI RCATGY 4 cut(s) 14, 1408, 2453, 3323
XmiI GTMKAC 3 cut(s) 216, 597, 1147
XmnI GAANNNNTTC 1 cut(s) 2022
XspI CTAG 9 cut(s) 165, 459, 554, 1118, 1769, 2129, 2292, 2394, 2879
ZrmI AGTACT 1 cut(s) 1793
Zsp2I ATGCAT 3 cut(s) 608, 852, 2860
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.