FvH4_3g38670

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000085
Physical Location & Seq
Reverse (-)
32957207 .. 32961555
4349 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g38670.t1

Sequence Viewer

Length: 3360 bp
ATGGCCTCAAGCAGCCACAGAGCCTCTGCATCTCCTACTTCAGCTGAGTCACCAGATTATTGGTGGAAGTACGATGTGTTTCTTAGCTTCAGGGGTGCAGACATTCGTGACAATTTTCTCTCCCACTTATACCATGAATTGCAAAAAATAAGAAGCATAAATACATTCAAGGATGACAAAGAGCTTGAAATAGGGGCCCCTATTTCTCCAAGTCTCCTAAAGGCAATTGAAGAATCAAGGCTTGCGATTGTTGTTCTCTCCCGGAACTATGCTTCCTCTAGTTGGTGTTTGGAGGAACTCACAAAGATCTGTCAATGCATGAAAGACAGCAATAGAATTCTGCCAGTATTTTATCATGTAGATCCCTCTGAGCTACGATATCATAAGAGGAGTTTTGCAGAAGCTTTCACTAAGCGTAAAACGTCTAAGAAACACATAAAGAAGGTGAAGCAAAAGAAGGTGCAGCAAAAGAAAGTGCAGCAGTGGAGAAATGCTTTAATTAAAGTGTCCAATTTCTCTGGGTGGCATACACAGAATTATAAGACTGAAAGAGAACTTATCGAAGTCATTGTGGAGTTCGTGTGCAGTAAGGTGCGATCTGTTTCATTGTCCCCGGGGCATTTTATAGCATTTGGAACAACTAGAGAAGCCATGAATGAAGTCATGGAGGCGCTTAAAGATGATCAAGTCACTGCCATTGCGGTGTACGGAATGGGAGGCGTTGGGAAGACAACTATGGTGGAACATGTTGGTGTTCAAGCCCAAAATAATGGGACTGTTAATCATGTGATAATGGTTATCATATCCCAAAAGCCCAACTTGAAAAGTATACAAGGCACATTGGCAGATCTGCTGGGTTTGAAACTGCGGAATGAGTCGACAATTGGGAGGGCCCAAGAAGTGAAGGGGGAGATAATGAGGAGAAAAAATATCCTTTTCATCCTCGACGATATTTGGAGCAGAATAAAGTTGTCAAGCATAGGGATTCCCAGCTATTACGAGCTTCGAAAATGCAACTCCAAAGTTCTACTCACCACTAGGATACTGCATGTTTGTCATTCCATGGAGATCCAACGAAGTATTCCTCTCAATATTTTATCAGAAGAAGATTCTTGGAATTTGTTGGTGAAAGAAGCAAGAAAGTGTTTTGAAACTTCAGCTGAATTTTATGATGTAGCCCGGAAGGTAGCTAGTGAGTGTGCTGGTCTCCCTCTTGCGTTGACAACAGTTGCGAGGGCACTAGGAGATAAAGATTTTGAAGAATGGAAAGAAGCAGCTAGACGACTTGAGATGTCTCAACGTGCCAACATAGATGATGATGGAGATGTGTTTGAATGTTTCAAATTAAGCTACGATTACTTGAACAATGATCATGTCAAGTCATGTTTCTTGCTCTGTTGCCTATTCCCAGAAAATTCTGATATCCCAATAGAAGACTTGGTGAAGTACGGGTTTGGGAAAGGATTGTTTCGAGATGTCAACAACACACTAGAAAATGCCAGAGTCATAGTAAATTCGGCTACGAAATGCCTTAAAGCTGCTTGTTTGCTTTTGGACAGTACAAAGGATGGTTGCATAAGGATGCATGATGTTATTCGAGATGTGGCCATGTCAATTGCATTGTCTGAAGATGGTCATGGCTTTTTTGTTAAAGCTCACTCTGAATTGAAAGAGTGGCCGATGGATGAACATGAGAGCTACTCAGGAGTCTCGCTGATAGAAAACTACAGTTGCAATCTTCCCGAAGAGTTGGTATGTCCAAACCTCCAGATTTTATTACTTAAAGGTACTAAATCAATAACAAAGATACCAAAATCATTTTTTCAAAGTTCAACTGCATTGATGGTCTTGGATCTTAGTGATACTAGTATTTCTTCACTACCCCCATCATTTAATCTTCTAACAAACCTTCAAGCTCTGTACTTGGATTATTGCAAGTCACTAATTGATATATCCATACTTAGAGCACTGAAGAAACTTGAAATTCTCAGTATGAGAGAATATCCCAACAAAGAGTTTCCTAAAGAAATAGGATACTTGACTTGTTTAAGGATGTTAGACATCACAAATGGAGATGTTGATACAATTCCATCTCAAGTAATTTCAAGGTTGCTTAGAGTAGAAGAACTGTACTTGCAGTGTGAGTTTGGGGACTGGACTAGTAAAGATGAGGGATGCTGTTCTTTCTTTTTTGGTAAAAATGGTGGCTTCCTGGATTTAACTAGCTTGTCATATTTAAAAATTTTGGAGGTCTCCATAGCTGGTGCAGATTGCTTGCCTACAAATATTGACTTGGATCCAAATTGGGAAAGCTTTTACATACATATCAACAGAAACAACAACATGAGAAGATCCACTCTGGAACGTGACCGTCCACATTATCATTCAAGATCCCTGAGTCTTGACACAACCATGACTACACTACCGGATTGGTTCATTAACCTGGTGACAAAGAAAGCAGAGACATTGTCTTATGTTGAGTGCAAGGGTTTAAATAGCATTCTCGTGGAACATGACCACGGGAGACTACACAGATTGAAGCGGCTCACTGTGTTTGATGACATAAACTTGGAAGAGTTCTTCAACCTGACAACAAGGGTTTCAACAGAACCTGTGTTCCAGAGCTTAGAAGAATTGCATCTCATTAGCATGTTTTGCCTGAAGGAGTTATGTGTTGGTGAACTACCAGTTGGATCTTTATGCACTCTGAAGATATTGGAGATAAGAGGTTGTCGTAATTTGGTGAACGCACTTTTACCGTTAGAATTGTTGCAGAGACTACAAAGTCTGGAAATAATTTGTCATAGTTCAAGTCAAATAGAACATGTGTTTGGTTTTGAAGGGGTGGAGCCAGAAAAGATTATCTTGAAAAAATTTAGAGAAATGAGATTGTATGATCTACATAAACTGATAAGCATATCGAGCGGTCCTGCTCCACAGGCAATCTTCCATAATCTTAGAAGTTTGGCACTGAACGGGTGCAAACTAAAGTATCTTTTCACAATTGATGTAGCACAGTATCTTTTGCTATTGGAAGACCTTTGGCTACAGAATTGCTATAAGTTGGAAGGAGTTATTGAATCAAGAGAAGAAACAGTGAACAATAAAATGATCATCTTTCCAGAATTGAAGAGCTTACATTTGCAAGATCTTCCACATATGAAATGGTTCTGCAGTCCTACTAAAAGTGCTACTAATCCAAAAGATACCGAATGCCCTTCATTACAACACTTGTATATGCGTGACTGCCCTCATCTGTCACTCACTGCTTCTGACTTCAACACCAGAAACCCTGTACAAATCAATAACGAGAAGCAGAGGTCGTCTATGTACAAAAGATTGTGGGACTCGGGCAAATAA

Protein Analysis

1120

Amino Acids

127.86

Weight (kDa)

6.8

Isoelectric Point (pI)

50.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 23 - 202 3.8e-45 TIR domain
TIR_2 PF13676 26 - 130 1.1e-13 TIR domain
NB-ARC PF00931 216 - 379 1e-26 NB-ARC domain
LRR_8 PF13855 587 - 645 1.7e-09 Leucine rich repeat
LRR_14 PF23598 589 - 690 8.1e-11 Leucine-rich repeat region
LRR_14 PF23598 649 - 712 7.1e-06 Leucine-rich repeat region
LRR_RPS2 PF23247 876 - 1019 9.6e-19 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 977 - 1087 2.9e-10 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 540
AasI GACNNNNNNGTC 1 cut(s) 2784
AccBSI CCGCTC 1 cut(s) 2925
AccI GTMKAC 2 cut(s) 829, 878
AciI CCGC 4 cut(s) 701, 868, 2542, 2925
AclWI GGATC 8 cut(s) 356, 1063, 1860, 2291, 2304, 2346, 2385, 2701
AcoI YGGCCR 2 cut(s) 1605, 1676
AcsI RAATTY 8 cut(s) 336, 1117, 1163, 1414, 1513, 1983, 2241, 2873
AcuI CTGAAG 7 cut(s) 24, 73, 1140, 1647, 1991, 2681, 2729
AfaI GTAC 9 cut(s) 71, 707, 1448, 1561, 1789, 1922, 2132, 3297, 3332
AfiI CCNNNNNNNGG 1 cut(s) 282
AflIII ACRYGT 2 cut(s) 745, 2824
AhlI ACTAGT 2 cut(s) 1865, 2159
AjnI CCWGG 2 cut(s) 2211, 2442
AjuI GAANNNNNNNTTGG 6 cut(s) 1065, 1097, 2673, 2705, 2814, 2846
AloI GAACNNNNNNTCC 4 cut(s) 257, 289, 2601, 2633
Alw21I GWGCWC 1 cut(s) 1969
Alw26I GTCTC 8 cut(s) 218, 1211, 1299, 1714, 2257, 2456, 2518, 2770
AlwI GGATC 8 cut(s) 356, 1063, 1860, 2291, 2304, 2346, 2385, 2701
AlwNI CAGNNNCTG 1 cut(s) 2612
Ama87I CYCGRG 2 cut(s) 613, 3349
AoxI GGCC 5 cut(s) 3, 195, 891, 1605, 1676
ApaI GGGCCC 2 cut(s) 199, 895
ApeKI GCWGC 5 cut(s) 12, 463, 478, 1274, 1538
ApoI RAATTY 8 cut(s) 336, 1117, 1163, 1414, 1513, 1983, 2241, 2873
Asp700I GAANNNNTTC 1 cut(s) 3167
AspLEI GCGC 1 cut(s) 673
AspS9I GGNCC 5 cut(s) 195, 196, 891, 892, 2927
AsuC2I CCSGG 4 cut(s) 262, 614, 615, 1180
AsuHPI GGTGA 8 cut(s) 42, 457, 1024, 1138, 1453, 2458, 2690, 2755
AsuII TTCGAA 1 cut(s) 1006
AvaI CYCGRG 2 cut(s) 613, 3349
AvaII GGWCC 1 cut(s) 2927
BaeGI GKGCMC 3 cut(s) 199, 895, 1240
BaeI ACNNNNGTAYC 2 cut(s) 2975, 3008
BalI TGGCCA 1 cut(s) 1607
BamHI GGATCC 1 cut(s) 2296
BanII GRGCYC 2 cut(s) 199, 895
BarI GAAGNNNNNNTAC 2 cut(s) 145, 177
BauI CACGAG 1 cut(s) 2504
BbsI GAAGAC 3 cut(s) 734, 1440, 3042
Bbv12I GWGCWC 1 cut(s) 1969
BbvI GCAGC 5 cut(s) 24, 475, 490, 1286, 1525
BccI CCATC 7 cut(s) 1313, 1562, 1625, 1675, 1837, 1894, 2098
BciT130I CCWGG 2 cut(s) 2213, 2444
BciVI GTATCC 2 cut(s) 1035, 2027
BclI TGATCA 3 cut(s) 682, 1369, 3111
BcnI CCSGG 4 cut(s) 262, 614, 615, 1180
BcoDI GTCTC 8 cut(s) 218, 1211, 1299, 1714, 2257, 2456, 2518, 2770
BcuI ACTAGT 2 cut(s) 1865, 2159
BfmI CTRYAG 3 cut(s) 1726, 3047, 3172
BfoI RGCGCY 1 cut(s) 674
BfuI GTATCC 2 cut(s) 1035, 2027
BglII AGATCT 3 cut(s) 306, 847, 3148
BisI GCNGC 6 cut(s) 13, 464, 479, 1275, 1539, 2543
BlsI GCNGC 6 cut(s) 14, 465, 480, 1276, 1540, 2544
Bme1390I CCNGG 6 cut(s) 262, 614, 615, 1180, 2213, 2444
Bme18I GGWCC 1 cut(s) 2927
BmeT110I CYCGRG 2 cut(s) 613, 3349
BmgT120I GGNCC 5 cut(s) 195, 196, 891, 892, 2927
BmiI GGNNCC 6 cut(s) 196, 197, 198, 893, 2298, 2850
BmrFI CCNGG 6 cut(s) 262, 614, 615, 1180, 2213, 2444
BmsI GCATC 4 cut(s) 38, 1572, 2165, 2647
BpiI GAAGAC 3 cut(s) 734, 1440, 3042
BplI GAGNNNNNCTC 2 cut(s) 1685, 1717
BpmI CTGGAG 1 cut(s) 1751
Bpu14I TTCGAA 1 cut(s) 1006
BpuEI CTTGAG 2 cut(s) 1307, 2079
BpuMI CCSGG 4 cut(s) 262, 614, 615, 1180
BsaI GGTCTC 2 cut(s) 1211, 2257
BsaJI CCNNGG 5 cut(s) 612, 613, 614, 1062, 2518
BsaWI WCCGGW 1 cut(s) 2425
BsaXI ACNNNNNCTCC 2 cut(s) 566, 596
Bsc4I CCNNNNNNNGG 1 cut(s) 282
Bse1I ACTGG 3 cut(s) 344, 2159, 2687
Bse3DI GCAATG 1 cut(s) 696
BseBI CCWGG 2 cut(s) 2213, 2444
BseDI CCNNGG 5 cut(s) 612, 613, 614, 1062, 2518
BseGI GGATG 7 cut(s) 178, 939, 1573, 1587, 1690, 2058, 2180
BseLI CCNNNNNNNGG 1 cut(s) 282
BseMI GCAATG 1 cut(s) 696
BseMII CTCAG 5 cut(s) 36, 360, 1716, 2002, 2387
BseNI ACTGG 3 cut(s) 344, 2159, 2687
BseRI GAGGAG 2 cut(s) 403, 934
BseSI GKGCMC 3 cut(s) 199, 895, 1240
BseXI GCAGC 5 cut(s) 24, 475, 490, 1286, 1525
BseYI CCCAGC 2 cut(s) 853, 989
BsgI GTGCAG 5 cut(s) 117, 482, 497, 604, 2286
BshFI GGCC 5 cut(s) 5, 197, 893, 1607, 1678
BsiHKAI GWGCWC 1 cut(s) 1969
BsiHKCI CYCGRG 2 cut(s) 613, 3349
BsiSI CCGG 4 cut(s) 262, 614, 1180, 2426
BslFI GGGAC 3 cut(s) 595, 787, 2165
BslI CCNNNNNNNGG 1 cut(s) 282
BsmAI GTCTC 8 cut(s) 218, 1211, 1299, 1714, 2257, 2456, 2518, 2770
BsmFI GGGAC 3 cut(s) 595, 787, 2165
BsmI GAATGC 2 cut(s) 2499, 3218
BsnI GGCC 5 cut(s) 5, 197, 893, 1607, 1678
Bso31I GGTCTC 2 cut(s) 1211, 2257
BsoBI CYCGRG 2 cut(s) 613, 3349
Bsp119I TTCGAA 1 cut(s) 1006
Bsp120I GGGCCC 2 cut(s) 195, 891
Bsp1286I GDGCHC 4 cut(s) 199, 895, 1240, 1969
Bsp1407I TGTACA 2 cut(s) 3295, 3330
Bsp19I CCATGG 1 cut(s) 1062
BspACI CCGC 4 cut(s) 701, 868, 2542, 2925
BspANI GGCC 5 cut(s) 5, 197, 893, 1607, 1678
BspCNI CTCAG 5 cut(s) 37, 361, 1715, 2001, 2388
BspLI GGNNCC 6 cut(s) 196, 197, 198, 893, 2298, 2850
BspMAI CTGCAG 1 cut(s) 3176
BspPI GGATC 8 cut(s) 356, 1063, 1860, 2291, 2304, 2346, 2385, 2701
BspQI GCTCTTC 1 cut(s) 3125
BspT104I TTCGAA 1 cut(s) 1006
BspTNI GGTCTC 2 cut(s) 1211, 2257
BsrBI CCGCTC 1 cut(s) 2925
BsrDI GCAATG 1 cut(s) 696
BsrGI TGTACA 2 cut(s) 3295, 3330
BsrI ACTGG 3 cut(s) 344, 2159, 2687
BssECI CCNNGG 5 cut(s) 612, 613, 614, 1062, 2518
BssNAI GTATAC 1 cut(s) 830
BssSI CACGAG 1 cut(s) 2504
BssT1I CCWWGG 1 cut(s) 1062
Bst1107I GTATAC 1 cut(s) 830
Bst2BI CACGAG 1 cut(s) 2504
Bst2UI CCWGG 2 cut(s) 2213, 2444
Bst6I CTCTTC 3 cut(s) 1740, 2568, 3125
BstAPI GCANNNNNTGC 1 cut(s) 2655
BstAUI TGTACA 2 cut(s) 3295, 3330
BstBI TTCGAA 1 cut(s) 1006
BstC8I GCNNGC 2 cut(s) 243, 2276
BstDSI CCRYGG 2 cut(s) 1062, 2518
BstF5I GGATG 7 cut(s) 178, 939, 1573, 1587, 1690, 2058, 2180
BstH2I RGCGCY 1 cut(s) 674
BstHHI GCGC 1 cut(s) 673
BstMAI GTCTC 8 cut(s) 218, 1211, 1299, 1714, 2257, 2456, 2518, 2770
BstMWI GCNNNNNNNGC 3 cut(s) 2655, 2922, 2939
BstNI CCWGG 2 cut(s) 2213, 2444
BstNSI RCATGY 4 cut(s) 749, 1052, 2653, 2828
BstSCI CCNGG 6 cut(s) 260, 612, 613, 1178, 2211, 2442
BstSFI CTRYAG 3 cut(s) 1726, 3047, 3172
BstSLI GKGCMC 3 cut(s) 199, 895, 1240
BstV1I GCAGC 5 cut(s) 24, 475, 490, 1286, 1525
BstV2I GAAGAC 3 cut(s) 734, 1440, 3042
BstXI CCANNNNNNTGG 2 cut(s) 60, 770
BstZ17I GTATAC 1 cut(s) 830
BsuI GTATCC 2 cut(s) 1035, 2027
BsuRI GGCC 5 cut(s) 5, 197, 893, 1607, 1678
BtgI CCRYGG 2 cut(s) 1062, 2518
BtsCI GGATG 7 cut(s) 178, 939, 1573, 1587, 1690, 2058, 2180
BtsI GCAGTG 4 cut(s) 488, 690, 2144, 3264
BtsIMutI CAGTG 8 cut(s) 488, 690, 1967, 2144, 2547, 2969, 3102, 3264
Cac8I GCNNGC 2 cut(s) 243, 2276
CaiI CAGNNNCTG 1 cut(s) 2612
CfoI GCGC 1 cut(s) 673
Cfr13I GGNCC 5 cut(s) 195, 196, 891, 892, 2927
Cfr9I CCCGGG 1 cut(s) 613
CsiI ACCWGGT 1 cut(s) 2442
Csp6I GTAC 9 cut(s) 70, 706, 1447, 1560, 1788, 1921, 2131, 3296, 3331
CspCI CAANNNNNGTGG 2 cut(s) 720, 755
CviQI GTAC 9 cut(s) 70, 706, 1447, 1560, 1788, 1921, 2131, 3296, 3331
DraI TTTAAA 2 cut(s) 2238, 2493
DrdI GACNNNNNNGTC 1 cut(s) 2784
DseDI GACNNNNNNGTC 1 cut(s) 2784
EaeI YGGCCR 2 cut(s) 1605, 1676
Eam1104I CTCTTC 3 cut(s) 1740, 2568, 3125
EarI CTCTTC 3 cut(s) 1740, 2568, 3125
Eco130I CCWWGG 1 cut(s) 1062
Eco24I GRGCYC 2 cut(s) 199, 895
Eco31I GGTCTC 2 cut(s) 1211, 2257
Eco32I GATATC 2 cut(s) 380, 1423
Eco47I GGWCC 1 cut(s) 2927
Eco57I CTGAAG 7 cut(s) 24, 73, 1140, 1647, 1991, 2681, 2729
Eco88I CYCGRG 2 cut(s) 613, 3349
EcoO109I RGGNCCY 3 cut(s) 195, 196, 891
EcoRI GAATTC 1 cut(s) 336
EcoRII CCWGG 2 cut(s) 2211, 2442
EcoRV GATATC 2 cut(s) 380, 1423
EcoT14I CCWWGG 1 cut(s) 1062
EcoT22I ATGCAT 2 cut(s) 320, 1587
EcoT38I GRGCYC 2 cut(s) 199, 895
ErhI CCWWGG 1 cut(s) 1062
FalI AAGNNNNNCTT 4 cut(s) 803, 835, 2849, 2881
FaqI GGGAC 3 cut(s) 595, 787, 2165
FauNDI CATATG 1 cut(s) 3159
FbaI TGATCA 3 cut(s) 682, 1369, 3111
FblI GTMKAC 2 cut(s) 829, 878
Fnu4HI GCNGC 6 cut(s) 13, 464, 479, 1275, 1539, 2543
FokI GGATG 7 cut(s) 185, 926, 1580, 1594, 1697, 2065, 2187
FriOI GRGCYC 2 cut(s) 199, 895
Fsp4HI GCNGC 6 cut(s) 13, 464, 479, 1275, 1539, 2543
GlaI GCGC 1 cut(s) 672
GluI GCNGC 6 cut(s) 13, 464, 479, 1275, 1539, 2543
GsaI CCCAGC 2 cut(s) 857, 993
GsuI CTGGAG 1 cut(s) 1751
HaeII RGCGCY 1 cut(s) 674
HaeIII GGCC 5 cut(s) 5, 197, 893, 1607, 1678
HapII CCGG 4 cut(s) 262, 614, 1180, 2426
HhaI GCGC 1 cut(s) 673
Hin6I GCGC 1 cut(s) 671
HinP1I GCGC 1 cut(s) 671
HincII GTYRAC 3 cut(s) 879, 1221, 1480
HindII GTYRAC 3 cut(s) 879, 1221, 1480
HindIII AAGCTT 2 cut(s) 402, 2311
HpaII CCGG 4 cut(s) 262, 614, 1180, 2426
HphI GGTGA 8 cut(s) 42, 457, 1024, 1138, 1453, 2458, 2690, 2755
Hpy166II GTNNAC 9 cut(s) 706, 830, 879, 1221, 1480, 2375, 2681, 2746, 3100
Hpy188I TCNGA 7 cut(s) 370, 1102, 1420, 1627, 1663, 2709, 3274
Hpy8I GTNNAC 9 cut(s) 706, 830, 879, 1221, 1480, 2375, 2681, 2746, 3100
Hpy99I CGWCG 1 cut(s) 950
HpyAV CCTTC 9 cut(s) 436, 451, 898, 1177, 1919, 2656, 2834, 3062, 3228
HpyCH4IV ACGT 3 cut(s) 422, 1300, 2365
HpyF10VI GCNNNNNNNGC 3 cut(s) 2655, 2922, 2939
HpySE526I ACGT 3 cut(s) 422, 1300, 2365
HspAI GCGC 1 cut(s) 671
Ksp22I TGATCA 3 cut(s) 682, 1369, 3111
LguI GCTCTTC 1 cut(s) 3125
LmnI GCTCC 3 cut(s) 957, 2848, 2938
Lsp1109I GCAGC 5 cut(s) 24, 475, 490, 1286, 1525
LweI GCATC 4 cut(s) 38, 1572, 2165, 2647
MabI ACCWGGT 1 cut(s) 2442
MaeII ACGT 3 cut(s) 422, 1300, 2365
MaeIII GTNAC 8 cut(s) 48, 107, 688, 1938, 2366, 2446, 3242, 3258
MbiI CCGCTC 1 cut(s) 2925
MfeI CAATTG 4 cut(s) 225, 882, 1614, 3003
MhlI GDGCHC 4 cut(s) 199, 895, 1240, 1969
MlsI TGGCCA 1 cut(s) 1607
MluNI TGGCCA 1 cut(s) 1607
MlyI GAGTC 6 cut(s) 56, 884, 1512, 1716, 2407, 3341
MmeI TCCRAC 3 cut(s) 1096, 2671, 3045
Mox20I TGGCCA 1 cut(s) 1607
Mph1103I ATGCAT 2 cut(s) 320, 1587
MroXI GAANNNNTTC 1 cut(s) 3167
MscI TGGCCA 1 cut(s) 1607
MslI CAYNNNNRTG 6 cut(s) 701, 750, 1580, 2411, 2504, 2648
Msp20I TGGCCA 1 cut(s) 1607
MspA1I CMGCKG 2 cut(s) 44, 1160
MspI CCGG 4 cut(s) 262, 614, 1180, 2426
MspR9I CCNGG 6 cut(s) 262, 614, 615, 1180, 2213, 2444
MunI CAATTG 4 cut(s) 225, 882, 1614, 3003
Mva1269I GAATGC 2 cut(s) 2499, 3218
MvaI CCWGG 2 cut(s) 2213, 2444
MwoI GCNNNNNNNGC 3 cut(s) 2655, 2922, 2939
NciI CCSGG 4 cut(s) 262, 614, 615, 1180
NcoI CCATGG 1 cut(s) 1062
NdeI CATATG 1 cut(s) 3159
NlaIV GGNNCC 6 cut(s) 196, 197, 198, 893, 2298, 2850
NmuCI GTSAC 8 cut(s) 48, 107, 688, 1938, 2366, 2446, 3242, 3258
NsiI ATGCAT 2 cut(s) 320, 1587
NspI RCATGY 4 cut(s) 749, 1052, 2653, 2828
NspV TTCGAA 1 cut(s) 1006
PacI TTAATTAA 1 cut(s) 501
PciI ACATGT 2 cut(s) 745, 2824
PciSI GCTCTTC 1 cut(s) 3125
PctI GAATGC 2 cut(s) 2499, 3218
PdmI GAANNNNTTC 1 cut(s) 3167
PfeI GAWTC 4 cut(s) 233, 985, 1109, 3080
PflFI GACNNNGTC 1 cut(s) 2467
PfoI TCCNGGA 2 cut(s) 260, 2211
PkrI GCNGC 6 cut(s) 14, 465, 480, 1276, 1540, 2544
PleI GAGTC 6 cut(s) 55, 883, 1511, 1715, 2406, 3341
PpsI GAGTC 6 cut(s) 55, 883, 1511, 1715, 2406, 3341
PscI ACATGT 2 cut(s) 745, 2824
PsiI TTATAA 1 cut(s) 540
Psp6I CCWGG 2 cut(s) 2211, 2442
PspFI CCCAGC 2 cut(s) 853, 989
PspGI CCWGG 2 cut(s) 2211, 2442
PspN4I GGNNCC 6 cut(s) 196, 197, 198, 893, 2298, 2850
PspOMI GGGCCC 2 cut(s) 195, 891
PspPI GGNCC 5 cut(s) 195, 196, 891, 892, 2927
PstI CTGCAG 1 cut(s) 3176
PstNI CAGNNNCTG 1 cut(s) 2612
PsyI GACNNNGTC 1 cut(s) 2467
PvuII CAGCTG 2 cut(s) 44, 1160
RsaI GTAC 9 cut(s) 71, 707, 1448, 1561, 1789, 1922, 2132, 3297, 3332
RsaNI GTAC 9 cut(s) 70, 706, 1447, 1560, 1788, 1921, 2131, 3296, 3331
RseI CAYNNNNRTG 6 cut(s) 701, 750, 1580, 2411, 2504, 2648
SalI GTCGAC 1 cut(s) 877
SapI GCTCTTC 1 cut(s) 3125
SatI GCNGC 6 cut(s) 13, 464, 479, 1275, 1539, 2543
Sau96I GGNCC 5 cut(s) 195, 196, 891, 892, 2927
SchI GAGTC 6 cut(s) 56, 884, 1512, 1716, 2407, 3341
ScrFI CCNGG 6 cut(s) 262, 614, 615, 1180, 2213, 2444
SduI GDGCHC 4 cut(s) 199, 895, 1240, 1969
SexAI ACCWGGT 1 cut(s) 2442
SfaNI GCATC 4 cut(s) 38, 1572, 2165, 2647
SfcI CTRYAG 3 cut(s) 1726, 3047, 3172
SfuI TTCGAA 1 cut(s) 1006
SinI GGWCC 1 cut(s) 2927
SmaI CCCGGG 1 cut(s) 615
SmiMI CAYNNNNRTG 6 cut(s) 701, 750, 1580, 2411, 2504, 2648
SmlI CTYRAG 3 cut(s) 7, 1286, 2094
SmoI CTYRAG 3 cut(s) 7, 1286, 2094
SpeI ACTAGT 2 cut(s) 1865, 2159
SsiI CCGC 4 cut(s) 701, 868, 2542, 2925
SspI AATATT 2 cut(s) 1093, 2287
StyD4I CCNGG 6 cut(s) 260, 612, 613, 1178, 2211, 2442
StyI CCWWGG 1 cut(s) 1062
TaiI ACGT 3 cut(s) 425, 1303, 2368
TaqI TCGA 7 cut(s) 561, 878, 945, 1006, 1471, 1597, 2921
TatI WGTACW 5 cut(s) 1559, 1920, 2130, 3295, 3330
TauI GCSGC 1 cut(s) 2545
TfiI GAWTC 4 cut(s) 233, 985, 1109, 3080
TscAI CASTG 8 cut(s) 488, 697, 1974, 2144, 2554, 2976, 3102, 3271
TseFI GTSAC 8 cut(s) 48, 107, 688, 1938, 2366, 2446, 3242, 3258
TseI GCWGC 5 cut(s) 12, 463, 478, 1274, 1538
Tsp45I GTSAC 8 cut(s) 48, 107, 688, 1938, 2366, 2446, 3242, 3258
TspGWI ACGGA 1 cut(s) 723
TspMI CCCGGG 1 cut(s) 613
TspRI CASTG 8 cut(s) 488, 697, 1974, 2144, 2554, 2976, 3102, 3271
Tth111I GACNNNGTC 1 cut(s) 2467
VpaK11BI GGWCC 1 cut(s) 2927
XapI RAATTY 8 cut(s) 336, 1117, 1163, 1414, 1513, 1983, 2241, 2873
XceI RCATGY 4 cut(s) 749, 1052, 2653, 2828
XcmI CCANNNNNNNNNTGG 2 cut(s) 60, 3162
XmaI CCCGGG 1 cut(s) 613
XmiI GTMKAC 2 cut(s) 829, 878
XmnI GAANNNNTTC 1 cut(s) 3167
Zsp2I ATGCAT 2 cut(s) 320, 1587
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.