FvH4_3g35820

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000085
Physical Location & Seq
Forward (+)
30753585 .. 30757303
3719 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g35820.t1

Sequence Viewer

Length: 3366 bp
ATGGTGAAACTTGTCAGCACACAAGCTCGAGAACTTAGGCTTTTTCATTATGTGATTAGGGCTGTTGTAGCTCAGAGCCCTGAATTAAGAAATATTCAAGACACATTGGCCGATCAGCTGGGCTTAAAATTTGAGGAGGAGGAGACGGAAATTGGAAGAGCCGTTAGATTGAAGGAGAGGTTAATGGGAGGAAACAAGATCCTCATAGTCTTGGTCGACGTTTGGCGGAAAATAGATTTGTCAACCATAGGAATTCCCAATTACAATGAGCTTCAAACACGCAATTCCAAACTCATACTAACCTCAAGGAGCAAAAGTGTATGCTATTCCATGCAGTGTCAAGCAAGCATTCCTCTCCATATCTTATCAGAAGAAGATTCTTGGAACTTTTTTGTGGAGATATTAGGAAAAGTTTTTGAAGAGTCTACCGAGATGTATCATGTAGCAAGGATGGTAGCTAGAGAATGTGGTGGTCTCCCACTTGCACTGATAACAGTTGCTCGGACACTTGGAGATAAAGACATAGAAGACTGGAAAGAAGCAGCTTACCGACTAGACAAGTCCCAAACTGAGGAAGATGTATACAAATGCATAAAGTTCAGCTATAATTACTTGAGATCTGATGCAAAATCAATCTTCTTGCTTTGCTGCCTATTCCCAGAAGATTATGATATCCCAATCGAATACTTGCTCAAGTATGCAATTGGCAAAGGGTTATTTCAAGTTCTCAACACAATCCAAGAAGCCAGAGCTACAGCAAATTTGATGATCAAGTCCCTCAAAGCTTTCAGCCTACTTTTGGAAGGTACAAAGGAGGGATGTGTAAGGATGCATGCTGTAGTGCGGGATGTTGCCCTGTTGATTTCGTTTTCTGAAGATGGTCATAAATTCTTGGTAAAGGCTGGTTGTGAATTGCAACATTGGCCAAAGATTAATGCGCGTGGAGGCTACTCTGCAATTTCACTAATGAGGAACAAAATTTACAAGCTTCCGGGAGAGTTGGTATGCTCAAATCTCCAGATTTTATTACTACAAAATAACACTGGTATATGTGTGATCCCAAATAAATTTTTTCAGAGTATAAAGGGACTTATGGTCCTAGATCTTAGCTTCACCAGTATCTCATCTCTACCCTCGTCGTTCGGTCTCCTAACCAATCTCAAAGGTTTGTATTTGGATTATTGCGACAAGATCTTTGACATATCCATATTCGTAAATCTGAAGAAACTTGAGATTCTTAGCATGAAAGCATCTCCTCTTGAGGAGTTGCCAAGAGAAATAGGAGATTTGACCAGTCTAAGGATGCTCGACTTAAGTGGGAGTTACAATATTGGTAGGATTCCATCTAATATAATGTCAAGTTTGTATAAATTAGAAGAACTATACATGCAAGGAAATTTTGGGGACTGGGGAGGAGGACGGTACAACACTAATGCTGGTTTTGATGAGTTGACTAGCTTGCGGTATTTAAACATCGTGAAGCTTCGCATATCTGATGCACAGTGCCTGCCTAAAAATGTTGTCCTTAATTTCAACTGGGTAATTTTCGATATATGTATCAGCAGAGAATCACCATCTAGAGACTACTCTTCAAAACCATACTGTTCACATGATCTTTCAAGAACCTTGACTCTGAGCACAACAATTAACACCTTACCAGATTGGTTTATCGAAGTGGTAGCAGAGAAAGCAGAGAAACTACAGTACCTGAAGTGCAAAGGCCTAAAGAACGTTCTTATGGAACATGACAAAGGGAGGTTATGTGGGCTGAAGTATCTAGCTGTAATTGGTCCCCATGAGTACCTGGAAGTGTTGATGGAAACAATAACACGGGGGCCAAATGTTCCTGTGTTCAAGAACTTGGAAGAGTTGCATCTTCTTGGCGTGAGTTGTCTGAAGGAGTTATGTGTTGGGGAATTACCACATGGATCTCTTTTCAATCTGAAGTCATTAAAGGTGGAATATTGCCATGACTTGATGAACGCACTTTTGCAATCAAATTTGTTGCAAAGACTACAAAATCTGGAAAAGCTTCATTGCGAAGATATGAATGTCATGGAGTATGTGTTTGGATTTGAAGGGCTGGAGCCAGAACATATTATCTTGCCAAAATTGAGGGAGATGATATTGTGGGATCTAGAAAAACTGATAAGCATATGGAATGGTCCTGCTCCAAATTCAGTTTTCCATAATCTTAAAAGTTTGGCAGTCTCTGGGTGCAAAAAACTTAAAAGTCTCTTTACATATGAGGTAGCTAAATGTCTTTTGCAATTGGAAGACCTTTCGGTAACTCAATGTCCTAGCTTGGAGATGGTTATTGAACCAAGCAAGGAAGTAGTTAACAAAAAGATCATTTTTCCAGAATTGAAGAACTTAGCTTTATCGTATCTTCCACAGCTCACAAGGTTCTGCGGTAGTGGAACTACTACTATTGAGTGCCCTTCATTGGAACATTTGCATTTGCAGGACTGCCCACATTTTACAACTTCTACTTCTGACTTCCACTGCTCAAAGGCGCAATTCCAAGTCAATGATCTACGACACATGAGAATGAGCAAAGACATGGATAGCATCAATTTAGCTGGCCAGCATACTAAGTACATCCTTGAGAGCCTACCTCAGAATGTCAGAAAGCGAGTTGAGGTCATAAGAGAAATTGAGAACCAACATCATGAATTAGAGGCCGAGTTGCTCAAGGAGAGAGCTATACTTGAAGCTAAGTATCATAAATTGTATCAACCTCTATACAGCAAGCGATACTTGATTGTGAATGGTGTTGTTGAAGCCGAAGGAGTTGCCACTGAAGCAGCAGCAAATCAAGACGTCAAAACTGCAAAAGTGAGAGGAGTGCCTGATTTCTGGCTCAATACATTGAAGAACAATGAAGTGCTAGCCAAGGAGATTACCATGTGTGATGAAGAAGCTCTTAAATATCTTAGAGACATCAAGTGGTTTAAGATACATGATTCCAAGGGGTTCAGACTTGAGTTCTACTTTGACACCAATCCATTTTTAAAAAACTCTGTTCTGACAAAGACATATCACATGATTGATGAAGATGAACCATACAGCGCAGCAGGGATGGAGATTGAATGGTATCCGGCAAAATGCTTGACACAGAAGATTCTTAAGAAGAAGCGTAAGAAGGGATCGAAGAGTTTTAAGCCACTGACCAGAACTGAAAACTGTAAAAGTTTCTTCAATTTCTTTAGTCCACCACAAGTGTCCAAGGATGATGAAGCTATTGATAAATGTGTTGCCGAGGAACTCAAAAACCAGAAGAAACACAACTATGATGTCGGGTCAACCATTTGTTATAAGATCATCCCACATGCAGTCTCATGGTTTACTGGAGAAGCTTTTTAG

Protein Analysis

1122

Amino Acids

128.16

Weight (kDa)

6.97

Isoelectric Point (pI)

40.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 13 - 134 4.9e-15 NB-ARC domain
LRR_8 PF13855 337 - 395 2.2e-08 Leucine rich repeat
LRR_14 PF23598 405 - 496 4.4e-09 Leucine-rich repeat region
LRR_RPS2 PF23247 621 - 766 2.5e-31 Plant disease resistance protein RPS2-like, leucine-rich repeats
NAP PF00956 878 - 1119 1.2e-71 Nucleosome assembly protein (NAP)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3318
AatII GACGTC 1 cut(s) 2826
AccI GTMKAC 3 cut(s) 216, 425, 582
AccII CGCG 1 cut(s) 940
AciI CCGC 4 cut(s) 226, 844, 1462, 2412
AclI AACGTT 1 cut(s) 1731
AclWI GGATC 5 cut(s) 193, 1051, 1936, 2142, 3157
AcoI YGGCCR 3 cut(s) 108, 923, 2584
AcsI RAATTY 9 cut(s) 128, 252, 760, 887, 978, 1067, 1396, 1999, 2176
AcuI CTGAAG 7 cut(s) 894, 1241, 1730, 1790, 1916, 1964, 2823
AcyI GRCGYC 1 cut(s) 2823
AfaI GTAC 5 cut(s) 808, 1424, 1706, 1802, 2600
AfiI CCNNNNNNNGG 4 cut(s) 571, 799, 1299, 2446
AflII CTTAAG 2 cut(s) 1312, 3128
AhdI GACNNNNNGTC 1 cut(s) 1094
AjnI CCWGG 1 cut(s) 1803
Alw21I GWGCWC 1 cut(s) 1640
Alw26I GTCTC 8 cut(s) 137, 479, 1151, 1575, 2215, 2240, 2934, 3343
AlwI GGATC 5 cut(s) 193, 1051, 1936, 2142, 3157
AlwNI CAGNNNCTG 3 cut(s) 1507, 1708, 2213
Ama87I CYCGRG 1 cut(s) 27
AoxI GGCC 6 cut(s) 108, 923, 1720, 1835, 2584, 2682
ApeKI GCWGC 5 cut(s) 542, 648, 2807, 2810, 3074
ApoI RAATTY 9 cut(s) 128, 252, 760, 887, 978, 1067, 1396, 1999, 2176
ArsI GACNNNNNNTTYG 2 cut(s) 1178, 1210
AseI ATTAAT 1 cut(s) 933
Asp700I GAANNNNTTC 1 cut(s) 2031
AspLEI GCGC 3 cut(s) 940, 2518, 3074
AspS9I GGNCC 4 cut(s) 1096, 1790, 1835, 2165
AsuC2I CCSGG 1 cut(s) 993
AsuHPI GGTGA 3 cut(s) 16, 1105, 1563
AsuNHI GCTAGC 1 cut(s) 2890
AvaI CYCGRG 1 cut(s) 27
AvaII GGWCC 3 cut(s) 1096, 1790, 2165
BaeGI GKGCMC 1 cut(s) 2441
BaeI ACNNNNGTAYC 2 cut(s) 1688, 1721
BalI TGGCCA 2 cut(s) 925, 2586
BanII GRGCYC 1 cut(s) 80
BbsI GAAGAC 2 cut(s) 534, 2283
Bbv12I GWGCWC 1 cut(s) 1640
BbvI GCAGC 5 cut(s) 554, 635, 2819, 2822, 3086
BccI CCATC 7 cut(s) 445, 872, 1351, 1582, 1810, 2305, 3076
BceAI ACGGC 1 cut(s) 146
BcgI CGANNNNNNTGC 2 cut(s) 2777, 2811
BciT130I CCWGG 1 cut(s) 1805
BciVI GTATCC 1 cut(s) 3108
BclI TGATCA 1 cut(s) 768
BcnI CCSGG 1 cut(s) 993
BcoDI GTCTC 8 cut(s) 137, 479, 1151, 1575, 2215, 2240, 2934, 3343
BfaI CTAG 9 cut(s) 459, 554, 1100, 1455, 1578, 1778, 2138, 2301, 2891
BfmI CTRYAG 3 cut(s) 753, 837, 1700
BfrI CTTAAG 2 cut(s) 1312, 3128
BfuI GTATCC 1 cut(s) 3108
BglII AGATCT 3 cut(s) 617, 1102, 1191
BisI GCNGC 5 cut(s) 543, 649, 2808, 2811, 3075
BlsI GCNGC 5 cut(s) 544, 650, 2809, 2812, 3076
Bme1390I CCNGG 2 cut(s) 993, 1805
Bme18I GGWCC 3 cut(s) 1096, 1790, 2165
BmeRI GACNNNNNGTC 1 cut(s) 1094
BmeT110I CYCGRG 1 cut(s) 27
BmgT120I GGNCC 4 cut(s) 1096, 1790, 1835, 2165
BmiI GGNNCC 3 cut(s) 1792, 1836, 2088
BmrFI CCNGG 2 cut(s) 993, 1805
BmrI ACTGGG 2 cut(s) 1417, 1546
BmsI GCATC 7 cut(s) 613, 819, 1259, 1293, 1486, 1882, 2580
BmtI GCTAGC 1 cut(s) 2894
BmuI ACTGGG 2 cut(s) 1417, 1546
BpiI GAAGAC 2 cut(s) 534, 2283
BplI GAGNNNNNCTC 2 cut(s) 2602, 2634
BpmI CTGGAG 2 cut(s) 1001, 2105
BpuEI CTTGAG 8 cut(s) 289, 634, 677, 1250, 1280, 2627, 2678, 3005
BpuMI CCSGG 1 cut(s) 993
BsaHI GRCGYC 1 cut(s) 2823
BsaI GGTCTC 2 cut(s) 479, 1151
BsaJI CCNNGG 4 cut(s) 2895, 2970, 3228, 3261
BsaXI ACNNNNNCTCC 8 cut(s) 301, 331, 987, 1017, 1747, 1777, 2051, 2081
Bsc4I CCNNNNNNNGG 4 cut(s) 571, 799, 1299, 2446
Bse1I ACTGG 7 cut(s) 536, 1048, 1116, 1293, 1412, 1541, 3355
Bse3DI GCAATG 1 cut(s) 2035
BseBI CCWGG 1 cut(s) 1805
BseDI CCNNGG 4 cut(s) 2895, 2970, 3228, 3261
BseGI GGATG 9 cut(s) 456, 824, 834, 853, 1308, 2601, 3087, 3238, 3324
BseLI CCNNNNNNNGG 4 cut(s) 571, 799, 1299, 2446
BseMI GCAATG 1 cut(s) 2035
BseMII CTCAG 4 cut(s) 86, 561, 1625, 2633
BseNI ACTGG 7 cut(s) 536, 1048, 1116, 1293, 1412, 1541, 3355
BseRI GAGGAG 7 cut(s) 149, 152, 155, 1245, 1277, 1428, 2859
BseSI GKGCMC 1 cut(s) 2441
BseXI GCAGC 5 cut(s) 554, 635, 2819, 2822, 3086
BseYI CCCAGC 1 cut(s) 118
Bsh1236I CGCG 1 cut(s) 940
BshFI GGCC 6 cut(s) 110, 925, 1722, 1837, 2586, 2684
BsiHKAI GWGCWC 1 cut(s) 1640
BsiHKCI CYCGRG 1 cut(s) 27
BsiSI CCGG 2 cut(s) 992, 3101
BslFI GGGAC 5 cut(s) 547, 760, 1101, 1418, 1776
BslI CCNNNNNNNGG 4 cut(s) 571, 799, 1299, 2446
BsmAI GTCTC 8 cut(s) 137, 479, 1151, 1575, 2215, 2240, 2934, 3343
BsmBI CGTCTC 1 cut(s) 137
BsmFI GGGAC 5 cut(s) 547, 760, 1101, 1418, 1776
BsmI GAATGC 1 cut(s) 348
BsnI GGCC 6 cut(s) 110, 925, 1722, 1837, 2586, 2684
Bso31I GGTCTC 2 cut(s) 479, 1151
BsoBI CYCGRG 1 cut(s) 27
Bsp1286I GDGCHC 3 cut(s) 80, 1640, 2441
BspACI CCGC 4 cut(s) 226, 844, 1462, 2412
BspANI GGCC 6 cut(s) 110, 925, 1722, 1837, 2586, 2684
BspCNI CTCAG 4 cut(s) 85, 562, 1626, 2632
BspFNI CGCG 1 cut(s) 940
BspHI TCATGA 1 cut(s) 2671
BspLI GGNNCC 3 cut(s) 1792, 1836, 2088
BspOI GCTAGC 1 cut(s) 2894
BspPI GGATC 5 cut(s) 193, 1051, 1936, 2142, 3157
BspQI GCTCTTC 1 cut(s) 151
BspTI CTTAAG 2 cut(s) 1312, 3128
BspTNI GGTCTC 2 cut(s) 479, 1151
BsrDI GCAATG 1 cut(s) 2035
BsrI ACTGG 7 cut(s) 536, 1048, 1116, 1293, 1412, 1541, 3355
BssECI CCNNGG 4 cut(s) 2895, 2970, 3228, 3261
BssNAI GTATAC 1 cut(s) 583
BssNI GRCGYC 1 cut(s) 2823
BssT1I CCWWGG 3 cut(s) 2895, 2970, 3228
Bst1107I GTATAC 1 cut(s) 583
Bst2UI CCWGG 1 cut(s) 1805
Bst4CI ACNGT 6 cut(s) 496, 1422, 1503, 1604, 1704, 3188
Bst6I CTCTTC 5 cut(s) 151, 414, 1594, 1860, 3149
BstACI GRCGYC 1 cut(s) 2823
BstAFI CTTAAG 2 cut(s) 1312, 3128
BstC8I GCNNGC 8 cut(s) 346, 834, 1460, 1508, 2584, 2588, 2753, 2892
BstF5I GGATG 9 cut(s) 456, 824, 834, 853, 1308, 2601, 3087, 3238, 3324
BstFNI CGCG 1 cut(s) 940
BstHHI GCGC 3 cut(s) 940, 2518, 3074
BstMAI GTCTC 8 cut(s) 137, 479, 1151, 1575, 2215, 2240, 2934, 3343
BstMWI GCNNNNNNNGC 4 cut(s) 68, 922, 1688, 2804
BstNI CCWGG 1 cut(s) 1805
BstNSI RCATGY 3 cut(s) 836, 1390, 3335
BstSCI CCNGG 2 cut(s) 991, 1803
BstSFI CTRYAG 3 cut(s) 753, 837, 1700
BstSLI GKGCMC 1 cut(s) 2441
BstUI CGCG 1 cut(s) 940
BstV1I GCAGC 5 cut(s) 554, 635, 2819, 2822, 3086
BstV2I GAAGAC 2 cut(s) 534, 2283
BstX2I RGATCY 6 cut(s) 198, 617, 1102, 1191, 1928, 2134
BstYI RGATCY 6 cut(s) 198, 617, 1102, 1191, 1928, 2134
BstZ17I GTATAC 1 cut(s) 583
BsuI GTATCC 1 cut(s) 3108
BsuRI GGCC 6 cut(s) 110, 925, 1722, 1837, 2586, 2684
BtsCI GGATG 9 cut(s) 456, 824, 834, 853, 1308, 2601, 3087, 3238, 3324
BtsI GCAGTG 2 cut(s) 341, 2503
BtsIMutI CAGTG 7 cut(s) 341, 485, 1041, 1508, 2503, 2799, 3167
Cac8I GCNNGC 8 cut(s) 346, 834, 1460, 1508, 2584, 2588, 2753, 2892
CaiI CAGNNNCTG 3 cut(s) 1507, 1708, 2213
CciI TCATGA 1 cut(s) 2671
CfoI GCGC 3 cut(s) 940, 2518, 3074
Cfr13I GGNCC 4 cut(s) 1096, 1790, 1835, 2165
Csp6I GTAC 5 cut(s) 807, 1423, 1705, 1801, 2599
CviQI GTAC 5 cut(s) 807, 1423, 1705, 1801, 2599
DraI TTTAAA 2 cut(s) 1470, 3015
DriI GACNNNNNGTC 1 cut(s) 1094
EaeI YGGCCR 3 cut(s) 108, 923, 2584
Eam1104I CTCTTC 5 cut(s) 151, 414, 1594, 1860, 3149
Eam1105I GACNNNNNGTC 1 cut(s) 1094
EarI CTCTTC 5 cut(s) 151, 414, 1594, 1860, 3149
EciI GGCGGA 1 cut(s) 241
Eco130I CCWWGG 3 cut(s) 2895, 2970, 3228
Eco147I AGGCCT 1 cut(s) 1722
Eco24I GRGCYC 1 cut(s) 80
Eco31I GGTCTC 2 cut(s) 479, 1151
Eco32I GATATC 1 cut(s) 673
Eco47I GGWCC 3 cut(s) 1096, 1790, 2165
Eco57I CTGAAG 7 cut(s) 894, 1241, 1730, 1790, 1916, 1964, 2823
Eco88I CYCGRG 1 cut(s) 27
EcoRI GAATTC 1 cut(s) 252
EcoRII CCWGG 1 cut(s) 1803
EcoRV GATATC 1 cut(s) 673
EcoT14I CCWWGG 3 cut(s) 2895, 2970, 3228
EcoT22I ATGCAT 2 cut(s) 593, 834
EcoT38I GRGCYC 1 cut(s) 80
ErhI CCWWGG 3 cut(s) 2895, 2970, 3228
Esp3I CGTCTC 1 cut(s) 137
FalI AAGNNNNNCTT 4 cut(s) 2744, 2776, 2910, 2942
FaqI GGGAC 5 cut(s) 547, 760, 1101, 1418, 1776
FauI CCCGC 1 cut(s) 837
FauNDI CATATG 2 cut(s) 2156, 2245
FbaI TGATCA 1 cut(s) 768
FblI GTMKAC 3 cut(s) 216, 425, 582
Fnu4HI GCNGC 5 cut(s) 543, 649, 2808, 2811, 3075
FokI GGATG 9 cut(s) 463, 831, 841, 860, 1315, 2588, 3094, 3245, 3311
FriOI GRGCYC 1 cut(s) 80
Fsp4HI GCNGC 5 cut(s) 543, 649, 2808, 2811, 3075
FspBI CTAG 9 cut(s) 459, 554, 1100, 1455, 1578, 1778, 2138, 2301, 2891
GlaI GCGC 3 cut(s) 939, 2517, 3073
GluI GCNGC 5 cut(s) 543, 649, 2808, 2811, 3075
GsaI CCCAGC 1 cut(s) 122
GsuI CTGGAG 2 cut(s) 1001, 2105
HaeIII GGCC 6 cut(s) 110, 925, 1722, 1837, 2586, 2684
HapII CCGG 2 cut(s) 992, 3101
HhaI GCGC 3 cut(s) 940, 2518, 3074
Hin1I GRCGYC 1 cut(s) 2823
Hin6I GCGC 3 cut(s) 938, 2516, 3072
HinP1I GCGC 3 cut(s) 938, 2516, 3072
HincII GTYRAC 5 cut(s) 217, 243, 1452, 2341, 3306
HindII GTYRAC 5 cut(s) 217, 243, 1452, 2341, 3306
HindIII AAGCTT 5 cut(s) 783, 986, 1481, 2030, 3357
HinfI GANTC 8 cut(s) 377, 422, 1234, 1339, 1568, 1630, 2966, 3124
HpaI GTTAAC 1 cut(s) 2341
HpaII CCGG 2 cut(s) 992, 3101
HphI GGTGA 3 cut(s) 16, 1105, 1563
Hpy99I CGWCG 2 cut(s) 221, 1141
HpyAV CCTTC 7 cut(s) 166, 797, 1891, 2072, 2451, 2783, 3139
HpyCH4III ACNGT 6 cut(s) 496, 1422, 1503, 1604, 1704, 3188
HpyCH4IV ACGT 3 cut(s) 219, 1731, 2823
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 922, 1688, 2804
HpySE526I ACGT 3 cut(s) 219, 1731, 2823
Hsp92I GRCGYC 1 cut(s) 2823
HspAI GCGC 3 cut(s) 938, 2516, 3072
Ksp22I TGATCA 1 cut(s) 768
KspAI GTTAAC 1 cut(s) 2341
LguI GCTCTTC 1 cut(s) 151
LmnI GCTCC 3 cut(s) 309, 2086, 2176
Lsp1109I GCAGC 5 cut(s) 554, 635, 2819, 2822, 3086
LweI GCATC 7 cut(s) 613, 819, 1259, 1293, 1486, 1882, 2580
MaeI CTAG 9 cut(s) 459, 554, 1100, 1455, 1578, 1778, 2138, 2301, 2891
MaeII ACGT 3 cut(s) 219, 1731, 2823
MaeIII GTNAC 2 cut(s) 1322, 2287
MfeI CAATTG 2 cut(s) 702, 2270
MflI RGATCY 6 cut(s) 198, 617, 1102, 1191, 1928, 2134
MhlI GDGCHC 3 cut(s) 80, 1640, 2441
MlsI TGGCCA 2 cut(s) 925, 2586
MluNI TGGCCA 2 cut(s) 925, 2586
MlyI GAGTC 2 cut(s) 431, 1624
Mox20I TGGCCA 2 cut(s) 925, 2586
Mph1103I ATGCAT 2 cut(s) 593, 834
MroXI GAANNNNTTC 1 cut(s) 2031
MscI TGGCCA 2 cut(s) 925, 2586
MslI CAYNNNNRTG 2 cut(s) 2549, 3291
Msp20I TGGCCA 2 cut(s) 925, 2586
MspA1I CMGCKG 1 cut(s) 118
MspCI CTTAAG 2 cut(s) 1312, 3128
MspI CCGG 2 cut(s) 992, 3101
MspR9I CCNGG 2 cut(s) 993, 1805
MunI CAATTG 2 cut(s) 702, 2270
Mva1269I GAATGC 1 cut(s) 348
MvaI CCWGG 1 cut(s) 1805
MvnI CGCG 1 cut(s) 940
MwoI GCNNNNNNNGC 4 cut(s) 68, 922, 1688, 2804
NciI CCSGG 1 cut(s) 993
NdeI CATATG 2 cut(s) 2156, 2245
NheI GCTAGC 1 cut(s) 2890
NlaIV GGNNCC 3 cut(s) 1792, 1836, 2088
NmeAIII GCCGAG 2 cut(s) 2710, 3286
NsiI ATGCAT 2 cut(s) 593, 834
NspI RCATGY 3 cut(s) 836, 1390, 3335
PaeI GCATGC 1 cut(s) 836
PaeR7I CTCGAG 1 cut(s) 27
PagI TCATGA 1 cut(s) 2671
PceI AGGCCT 1 cut(s) 1722
PciSI GCTCTTC 1 cut(s) 151
PctI GAATGC 1 cut(s) 348
PdmI GAANNNNTTC 1 cut(s) 2031
PfeI GAWTC 6 cut(s) 377, 1234, 1339, 1568, 2966, 3124
PfoI TCCNGGA 1 cut(s) 991
PkrI GCNGC 5 cut(s) 544, 650, 2809, 2812, 3076
PleI GAGTC 2 cut(s) 430, 1624
PpsI GAGTC 2 cut(s) 430, 1624
PshBI ATTAAT 1 cut(s) 933
PsiI TTATAA 1 cut(s) 3318
Psp1406I AACGTT 1 cut(s) 1731
Psp6I CCWGG 1 cut(s) 1803
PspFI CCCAGC 1 cut(s) 118
PspGI CCWGG 1 cut(s) 1803
PspN4I GGNNCC 3 cut(s) 1792, 1836, 2088
PspPI GGNCC 4 cut(s) 1096, 1790, 1835, 2165
PsrI GAACNNNNNNTAC 2 cut(s) 965, 997
PstNI CAGNNNCTG 3 cut(s) 1507, 1708, 2213
PsuI RGATCY 6 cut(s) 198, 617, 1102, 1191, 1928, 2134
PvuII CAGCTG 1 cut(s) 118
RsaI GTAC 5 cut(s) 808, 1424, 1706, 1802, 2600
RsaNI GTAC 5 cut(s) 807, 1423, 1705, 1801, 2599
RseI CAYNNNNRTG 2 cut(s) 2549, 3291
SalI GTCGAC 1 cut(s) 215
SapI GCTCTTC 1 cut(s) 151
SatI GCNGC 5 cut(s) 543, 649, 2808, 2811, 3075
Sau96I GGNCC 4 cut(s) 1096, 1790, 1835, 2165
SchI GAGTC 2 cut(s) 431, 1624
ScrFI CCNGG 2 cut(s) 993, 1805
SduI GDGCHC 3 cut(s) 80, 1640, 2441
SfaNI GCATC 7 cut(s) 613, 819, 1259, 1293, 1486, 1882, 2580
SfcI CTRYAG 3 cut(s) 753, 837, 1700
Sfr274I CTCGAG 1 cut(s) 27
SinI GGWCC 3 cut(s) 1096, 1790, 2165
SlaI CTCGAG 1 cut(s) 27
SmiMI CAYNNNNRTG 2 cut(s) 2549, 3291
SphI GCATGC 1 cut(s) 836
SseBI AGGCCT 1 cut(s) 1722
SsiI CCGC 4 cut(s) 226, 844, 1462, 2412
SspI AATATT 3 cut(s) 94, 1330, 1964
SspMI CTAG 9 cut(s) 459, 554, 1100, 1455, 1578, 1778, 2138, 2301, 2891
StuI AGGCCT 1 cut(s) 1722
StyD4I CCNGG 2 cut(s) 991, 1803
StyI CCWWGG 3 cut(s) 2895, 2970, 3228
TaaI ACNGT 6 cut(s) 496, 1422, 1503, 1604, 1704, 3188
TaiI ACGT 3 cut(s) 222, 1734, 2826
TaqI TCGA 7 cut(s) 28, 216, 681, 1308, 1548, 1671, 3152
TaqII GACCGA 1 cut(s) 1133
TatI WGTACW 1 cut(s) 2598
TfiI GAWTC 6 cut(s) 377, 1234, 1339, 1568, 2966, 3124
TscAI CASTG 7 cut(s) 341, 492, 1048, 1508, 2510, 2806, 3174
TseI GCWGC 5 cut(s) 542, 648, 2807, 2810, 3074
TspGWI ACGGA 1 cut(s) 161
TspRI CASTG 7 cut(s) 341, 492, 1048, 1508, 2510, 2806, 3174
Vha464I CTTAAG 2 cut(s) 1312, 3128
VpaK11BI GGWCC 3 cut(s) 1096, 1790, 2165
VspI ATTAAT 1 cut(s) 933
XapI RAATTY 9 cut(s) 128, 252, 760, 887, 978, 1067, 1396, 1999, 2176
XbaI TCTAGA 2 cut(s) 1577, 2137
XceI RCATGY 3 cut(s) 836, 1390, 3335
XhoI CTCGAG 1 cut(s) 27
XmiI GTMKAC 3 cut(s) 216, 425, 582
XmnI GAANNNNTTC 1 cut(s) 2031
XspI CTAG 9 cut(s) 459, 554, 1100, 1455, 1578, 1778, 2138, 2301, 2891
ZraI GACGTC 1 cut(s) 2824
Zsp2I ATGCAT 2 cut(s) 593, 834
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.