Rmu_sc0003069.1_g000053

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003069.1
OG0000085
Physical Location & Seq
Reverse (-)
232791 .. 242951
10161 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003069.1_g000053.1.cds

Sequence Viewer

Length: 4092 bp
atgtcagaggataatcaagaatcggttgatgttgatgcctctgcatctctctcttcatcatcagctcctcggtggaagcatgatgtatttttgagtttcaggggtgtagacactcgcaagggtattgcatccgaattatactatcgactgcaaaatgagagaggaatcaaaacatacatggatgaccaagaccttgaagtaggggatgttatttctcccactctcctaacggcaattaaagaatcaaggtttgcaattattgttctctctcaaaactatgcctcttccacttggtgtttggaggaacttagaaacatctgtgagtgcatggaagaagacaacaatagaattctgccacttttttattatgtggatcctactgatgtacgatatcagaagagaagtttcgaagatgctttcactaagcatgaaaaatcagagcgacaaagatcagagaaggtgcagcagtggagagatgctttaaaaaaagtggcaaatttctctgggtggcatacactgaattataaaactgaaagagaacttttggatgccattgaggaaactgtgtgcaacaaattacgacctactgaacttgagtttaaactgtccaacggagattttgaagcatttgcagcaacaaaacaagccattgatgaggttatgaatgcgctaaaagatgatgaggccactaccattggagtctacggaatggggggcgtcggaaaaacgacaatggtgaaatatgttggagcactagcccaagaaagtaggctttttaatcaagtgattatggctgtagtatcccaaaaccccgacttgatgaaaattcaacagacgtttgcagaaatgctgggctttaaattggaggagaagacagaaattgtaagagccattaaattgaaggagaagataatgagagagaccgggatcctcataatcttggatgacatttggaagagaatagacttttcagacattggaattccgagccacaacgaacttcaaaaatgcaattccaaagtcctactgaccaccagaagatcgagagtttgtaagatcatgaagagccatgcaaaaattcatctcaatatcttatcagaagaagatgcttggagcttatttgtgaaggaagtagggaatgcttttgacagatcttccaatttctatgatgtagcgagtgaggtggcaagagaatgcgccggtctaccagttgcgttgatagcagttgcgagggcccttcgagatgaaggtttggacgaatggaaagaagctgctcggcgactgaaagcatctcaacctcccatccctgaagatgagggagatgttttcaaatgcataaagttgagctatgattacttgaaatctgatgattccaaatcatgcttcttgctttgctgcctgttcccagaagattatgatatcccaattgaatacttggtcaagtatggaattgggaaaggaatgtttcaagattcaaacttgcgacaagcccgagccacaacaaattcagcggtgaaggcccttaaagagtctagcttgcttttggactctggcttgcttttgcactctatacatgacgtatatgtaaggatgcatgatgtcattcgggatatggcaatctcaattttgttatctgaagtacagcgggtcttggtgaaagctggctgtgaattgaagaattggccacagattgatgcacataaaggctactctgcaatctcactaatggagaacaaaatttgcaagctaccccaagagttggtatgtccaaatctccagattttattactgcaacacaatgcttcattaaatgatatcccagaatctttcttccaaagtcagaaagaattaagggtcttagatcttagctgcactggtatttcattactaccccaatcaatcagtttcctaaccaaccttcaagctttgtatttagattactgcaaggacataactgatatttctgtaatcggaaaacttcacaagcttgagattcttagtatgagaggaaatgctctgaaagaattgccaagagaaataggacagttgaccagtctaaggatgctggatatcactagagatgatagattccttttcatatccaggaatggatgtattggcacaattccatctaaagtgatatcaaagttgcataaattagaagaactgtacatgcaatgtgtattttgggactgggggcgtaaaattgatggagatggagaagaaactaatattggctttgatgaattggctggtttatcatatttaagcattttggaagtttgcatatctgatgcaaattgcatccctaaaactgttgaggtcgaaccgaattgggtttactttgatattagaatttctagcagacatacgagggaccatcagttcacacaatctggtcataattgtagatccttgactcttgatagtactgacaataccataggaacctttccggactggtttgtcaatgctgtggtaaagaatactgagaggctgcgttataaaaaatgcaaagggttaagtaacattctttcggaatatgaccgtgggaggcttcatggactgaaagttctctctgtaattggtcggtgtaagaacttgaaagaattgatgaatgcaataacatgtgttccagatatgcctgtgtttgagaacttggaaaagttgtatctggaacacctggatgacctgaagaagttatgtgttggtgagttaccacctggatctctccgcagtctgaaagtactgaaggtcagtcagtgtcctagcttggggaatgtactgttgccatcaaaattgttgccatcaaatttggaaatactaatctgtgagggaagtaaagtggaacatgtgtttggatttgaaggatttgagctggatcaaacaaatcttgtagtgatgaatttggagggtctatacgcagtaagaagcatatgtaatggtcctgctccacgtcgaatgttccaagctctgaaggaattggtcattacgaattgcaatttccagggaagcctcttcacatttgatgtagctcagtgtctttttcaattggaaacccttactgtatttgattgccctgtcttggaaagagtaatcgaagcaaggggggaaacattgaagaacgagaagaccattcttccaaaattgaagagcttaggtttgatagatcttccgatgttgtacaagggaggtgctactattgatttcgagtgtccttcattggaaaacttgtatctggagggctgcccccacttgtcatttccatcctctgcttctgactacttccacagcaggagccaagtcaatgataaggctgagtgtgtcccatttaaccacgtagagtatgtcagctggggaactgaagcttccagaattgacgactcgagagccaaccgctccggatttttcctcagccatctgaatttcagggatttcatcgttctcgaacttccggcgaagcccccgcaccacagaagccgaagaaagaacctaccgatgatgaaaagaggtaggggtgttcgtggtatctccgatataatcacgattcccgggctggcaaatgtcgactttgctgatgtaagggctataatggtgaatgcgggttcttcattgatggggataggaactgcaactgggaagacaagggcaagagatgctgctttaaatgccattcaatcatctttattagatattgttatagagagggctactggaattgtctggaacataactggtggaactgatttgacacactatgaggtaaatgctgcagcagaggttatatatgatcttgttgatccaacagcaaatttaatatttggagcagtgacagatccatcactcagtggtcaagttagcatcactctaattgctactggattcaaacgccaagaagaaagtgatgggaggccactccaggcacaagtagatattacccttggaatcaatcgaagaccttcctccttgtcagaaggtagttcagtcgagattcccgatttcttgaagaagaaaaggatgctcacgcatacttatcagtgcaaatgcaatcgctatcctgtcactatgatggtgaagaagttctacctttga

Protein Analysis

1363

Amino Acids

153.68

Weight (kDa)

5.98

Isoelectric Point (pI)

42.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 525, 2505
AasI GACNNNNNNGTC 1 cut(s) 2465
AccB7I CCANNNNNTGG 1 cut(s) 1759
AccBSI CCGCTC 1 cut(s) 3399
AccI GTMKAC 4 cut(s) 108, 702, 1204, 3567
AccIII TCCGGA 2 cut(s) 2455, 3401
AciI CCGC 6 cut(s) 1511, 1645, 2734, 3397, 3467, 3602
AclWI GGATC 9 cut(s) 368, 381, 922, 935, 2406, 2734, 2889, 3794, 3830
AcoI YGGCCR 1 cut(s) 1682
AcuI CTGAAG 6 cut(s) 1329, 1656, 2714, 2771, 2996, 3384
AcyI GRCGYC 1 cut(s) 717
AdeI CACNNNGTG 2 cut(s) 294, 3848
AfaI GTAC 7 cut(s) 387, 1641, 2171, 2431, 2748, 2784, 3185
AfiI CCNNNNNNNGG 4 cut(s) 1759, 2058, 2774, 3085
AflIII ACRYGT 2 cut(s) 2627, 2851
AjiI CACGTC 1 cut(s) 2957
AjnI CCWGG 5 cut(s) 2102, 2682, 2722, 3006, 3918
AjuI GAANNNNNNNTTGG 6 cut(s) 1367, 1399, 2217, 2249, 2841, 2873
AloI GAACNNNNNNTCC 4 cut(s) 2369, 2401, 2556, 2588
Alw21I GWGCWC 1 cut(s) 754
Alw26I GTCTC 1 cut(s) 914
AlwI GGATC 9 cut(s) 368, 381, 922, 935, 2406, 2734, 2889, 3794, 3830
Ama87I CYCGRG 3 cut(s) 1491, 3385, 3551
Aor13HI TCCGGA 2 cut(s) 2455, 3401
AoxI GGCC 5 cut(s) 684, 1233, 1518, 1682, 3911
ApaI GGGCCC 1 cut(s) 1237
Asp700I GAANNNNTTC 2 cut(s) 3958, 4079
AspLEI GCGC 2 cut(s) 670, 1199
AspS9I GGNCC 5 cut(s) 1233, 1234, 1519, 2377, 2945
AsuC2I CCSGG 3 cut(s) 925, 3552, 3553
AsuHPI GGTGA 6 cut(s) 748, 1525, 1666, 2723, 3607, 4084
AsuII TTCGAA 1 cut(s) 408
AvaI CYCGRG 3 cut(s) 1491, 3385, 3551
AvaII GGWCC 2 cut(s) 2377, 2945
BaeGI GKGCMC 1 cut(s) 1237
BalI TGGCCA 1 cut(s) 1684
BamHI GGATCC 2 cut(s) 373, 927
BanII GRGCYC 1 cut(s) 1237
BarI GAAGNNNNNNTAC 1 cut(s) 4067
BbsI GAAGAC 5 cut(s) 342, 878, 3137, 3647, 3961
Bbv12I GWGCWC 1 cut(s) 754
BbvCI CCTCAGC 1 cut(s) 3413
BceAI ACGGC 1 cut(s) 246
BciT130I CCWGG 5 cut(s) 2104, 2684, 2724, 3008, 3920
BciVI GTATCC 1 cut(s) 811
BcnI CCSGG 3 cut(s) 925, 3552, 3553
BcoDI GTCTC 1 cut(s) 914
BfaI CTAG 5 cut(s) 755, 1533, 2076, 2361, 2769
BfmI CTRYAG 2 cut(s) 795, 3771
BfuI GTATCC 1 cut(s) 811
BglII AGATCT 3 cut(s) 1151, 1861, 3169
BmcAI AGTACT 2 cut(s) 2431, 2748
Bme1390I CCNGG 8 cut(s) 925, 2104, 2684, 2724, 3008, 3552, 3553, 3920
Bme18I GGWCC 2 cut(s) 2377, 2945
BmeT110I CYCGRG 3 cut(s) 1491, 3385, 3551
BmgBI CACGTC 1 cut(s) 2957
BmgT120I GGNCC 5 cut(s) 1233, 1234, 1519, 2377, 2945
BmiI GGNNCC 6 cut(s) 375, 929, 1235, 2378, 2449, 3299
BmrFI CCNGG 8 cut(s) 925, 2104, 2684, 2724, 3008, 3552, 3553, 3920
BmrI ACTGGG 2 cut(s) 2203, 3645
BmuI ACTGGG 2 cut(s) 2203, 3645
BpiI GAAGAC 5 cut(s) 342, 878, 3137, 3647, 3961
BpmI CTGGAG 3 cut(s) 1760, 3260, 3902
Bpu10I CCTNAGC 2 cut(s) 3157, 3413
Bpu14I TTCGAA 1 cut(s) 408
BpuEI CTTGAG 2 cut(s) 614, 2009
BpuMI CCSGG 3 cut(s) 925, 3552, 3553
BsaAI YACGTR 1 cut(s) 3340
BsaHI GRCGYC 1 cut(s) 717
BsaI GGTCTC 1 cut(s) 914
BsaJI CCNNGG 5 cut(s) 68, 2548, 3007, 3551, 3940
BsaWI WCCGGW 2 cut(s) 2455, 3401
Bsc4I CCNNNNNNNGG 4 cut(s) 1759, 2058, 2774, 3085
Bse118I RCCGGY 1 cut(s) 1199
Bse1I ACTGG 9 cut(s) 1208, 1879, 2052, 2198, 2465, 3640, 3718, 3739, 3883
Bse3DI GCAATG 1 cut(s) 2183
BseAI TCCGGA 2 cut(s) 2455, 3401
BseBI CCWGG 5 cut(s) 2104, 2684, 2724, 3008, 3920
BseDI CCNNGG 5 cut(s) 68, 2548, 3007, 3551, 3940
BseLI CCNNNNNNNGG 4 cut(s) 1759, 2058, 2774, 3085
BseMI GCAATG 1 cut(s) 2183
BseMII CTCAG 5 cut(s) 2481, 3050, 3309, 3427, 3859
BseNI ACTGG 9 cut(s) 1208, 1879, 2052, 2198, 2465, 3640, 3718, 3739, 3883
BseRI GAGGAG 2 cut(s) 57, 881
BseSI GKGCMC 1 cut(s) 1237
BseYI CCCAGC 2 cut(s) 850, 3354
BsgI GTGCAG 2 cut(s) 482, 1855
BshFI GGCC 5 cut(s) 686, 1235, 1520, 1684, 3913
BsiHKAI GWGCWC 1 cut(s) 754
BsiHKCI CYCGRG 3 cut(s) 1491, 3385, 3551
BsiSI CCGG 6 cut(s) 924, 1200, 2456, 3402, 3455, 3552
BslFI GGGAC 3 cut(s) 2204, 2390, 3311
BslI CCNNNNNNNGG 4 cut(s) 1759, 2058, 2774, 3085
BsmAI GTCTC 1 cut(s) 914
BsmFI GGGAC 3 cut(s) 2204, 2390, 3311
BsmI GAATGC 5 cut(s) 670, 1144, 1199, 2623, 3604
BsnI GGCC 5 cut(s) 686, 1235, 1520, 1684, 3913
Bso31I GGTCTC 1 cut(s) 914
BsoBI CYCGRG 3 cut(s) 1491, 3385, 3551
Bsp119I TTCGAA 1 cut(s) 408
Bsp120I GGGCCC 1 cut(s) 1233
Bsp1286I GDGCHC 2 cut(s) 754, 1237
Bsp13I TCCGGA 2 cut(s) 2455, 3401
Bsp1407I TGTACA 2 cut(s) 2169, 3183
BspACI CCGC 6 cut(s) 1511, 1645, 2734, 3397, 3467, 3602
BspANI GGCC 5 cut(s) 686, 1235, 1520, 1684, 3913
BspCNI CTCAG 5 cut(s) 2482, 3049, 3310, 3426, 3858
BspEI TCCGGA 2 cut(s) 2455, 3401
BspHI TCATGA 1 cut(s) 1059
BspLI GGNNCC 6 cut(s) 375, 929, 1235, 2378, 2449, 3299
BspMAI CTGCAG 1 cut(s) 3775
BspPI GGATC 9 cut(s) 368, 381, 922, 935, 2406, 2734, 2889, 3794, 3830
BspQI GCTCTTC 2 cut(s) 1058, 3146
BspT104I TTCGAA 1 cut(s) 408
BspTNI GGTCTC 1 cut(s) 914
BsrBI CCGCTC 1 cut(s) 3399
BsrDI GCAATG 1 cut(s) 2183
BsrFI RCCGGY 1 cut(s) 1199
BsrGI TGTACA 2 cut(s) 2169, 3183
BsrI ACTGG 9 cut(s) 1208, 1879, 2052, 2198, 2465, 3640, 3718, 3739, 3883
BssAI RCCGGY 1 cut(s) 1199
BssECI CCNNGG 5 cut(s) 68, 2548, 3007, 3551, 3940
BssNI GRCGYC 1 cut(s) 717
BssT1I CCWWGG 1 cut(s) 3940
Bst2UI CCWGG 5 cut(s) 2104, 2684, 2724, 3008, 3920
Bst4CI ACNGT 8 cut(s) 565, 606, 2046, 2169, 2317, 2549, 2787, 3067
Bst6I CTCTTC 7 cut(s) 58, 289, 392, 950, 1058, 3023, 3146
BstACI GRCGYC 1 cut(s) 717
BstAPI GCANNNNNTGC 1 cut(s) 3656
BstAUI TGTACA 2 cut(s) 2169, 3183
BstBAI YACGTR 1 cut(s) 3340
BstBI TTCGAA 1 cut(s) 408
BstC8I GCNNGC 5 cut(s) 1538, 1556, 1663, 1745, 3558
BstDSI CCRYGG 1 cut(s) 2548
BstHHI GCGC 2 cut(s) 670, 1199
BstMAI GTCTC 1 cut(s) 914
BstMWI GCNNNNNNNGC 5 cut(s) 632, 1220, 1517, 3656, 3668
BstNI CCWGG 5 cut(s) 2104, 2684, 2724, 3008, 3920
BstNSI RCATGY 3 cut(s) 2176, 2631, 2855
BstSCI CCNGG 8 cut(s) 923, 2102, 2682, 2722, 3006, 3550, 3551, 3918
BstSFI CTRYAG 2 cut(s) 795, 3771
BstSLI GKGCMC 1 cut(s) 1237
BstV2I GAAGAC 5 cut(s) 342, 878, 3137, 3647, 3961
BstX2I RGATCY 8 cut(s) 373, 927, 1151, 1861, 2411, 2726, 3169, 3835
BstYI RGATCY 8 cut(s) 373, 927, 1151, 1861, 2411, 2726, 3169, 3835
BsuI GTATCC 1 cut(s) 811
BsuRI GGCC 5 cut(s) 686, 1235, 1520, 1684, 3913
BtgI CCRYGG 1 cut(s) 2548
BtrI CACGTC 1 cut(s) 2957
BtsI GCAGTG 2 cut(s) 473, 3834
BtsIMutI CAGTG 8 cut(s) 473, 515, 1872, 2768, 3044, 3834, 3853, 4043
Cac8I GCNNGC 5 cut(s) 1538, 1556, 1663, 1745, 3558
CciI TCATGA 1 cut(s) 1059
CfoI GCGC 2 cut(s) 670, 1199
Cfr10I RCCGGY 1 cut(s) 1199
Cfr13I GGNCC 5 cut(s) 1233, 1234, 1519, 2377, 2945
Cfr9I CCCGGG 1 cut(s) 3551
CseI GACGC 1 cut(s) 706
Csp6I GTAC 7 cut(s) 386, 1640, 2170, 2430, 2747, 2783, 3184
CspCI CAANNNNNGTGG 2 cut(s) 676, 711
CviQI GTAC 7 cut(s) 386, 1640, 2170, 2430, 2747, 2783, 3184
DraI TTTAAA 4 cut(s) 483, 601, 859, 3666
DraIII CACNNNGTG 2 cut(s) 294, 3848
DrdI GACNNNNNNGTC 1 cut(s) 2465
DseDI GACNNNNNNGTC 1 cut(s) 2465
EaeI YGGCCR 1 cut(s) 1682
Eam1104I CTCTTC 7 cut(s) 58, 289, 392, 950, 1058, 3023, 3146
EarI CTCTTC 7 cut(s) 58, 289, 392, 950, 1058, 3023, 3146
Eco130I CCWWGG 1 cut(s) 3940
Eco24I GRGCYC 1 cut(s) 1237
Eco31I GGTCTC 1 cut(s) 914
Eco32I GATATC 5 cut(s) 392, 1420, 1816, 2071, 2142
Eco47I GGWCC 2 cut(s) 2377, 2945
Eco57I CTGAAG 6 cut(s) 1329, 1656, 2714, 2771, 2996, 3384
Eco88I CYCGRG 3 cut(s) 1491, 3385, 3551
EcoO109I RGGNCCY 3 cut(s) 1233, 1234, 1519
EcoRI GAATTC 2 cut(s) 348, 981
EcoRII CCWGG 5 cut(s) 2102, 2682, 2722, 3006, 3918
EcoRV GATATC 5 cut(s) 392, 1420, 1816, 2071, 2142
EcoT14I CCWWGG 1 cut(s) 3940
EcoT22I ATGCAT 2 cut(s) 1337, 1596
EcoT38I GRGCYC 1 cut(s) 1237
ErhI CCWWGG 1 cut(s) 3940
FaqI GGGAC 3 cut(s) 2204, 2390, 3311
FauI CCCGC 3 cut(s) 1638, 3474, 3595
FauNDI CATATG 1 cut(s) 2936
FblI GTMKAC 4 cut(s) 108, 702, 1204, 3567
FriOI GRGCYC 1 cut(s) 1237
FspBI CTAG 5 cut(s) 755, 1533, 2076, 2361, 2769
GlaI GCGC 2 cut(s) 669, 1198
GsaI CCCAGC 2 cut(s) 854, 3358
GsuI CTGGAG 3 cut(s) 1760, 3260, 3902
HaeIII GGCC 5 cut(s) 686, 1235, 1520, 1684, 3913
HapII CCGG 6 cut(s) 924, 1200, 2456, 3402, 3455, 3552
HgaI GACGC 1 cut(s) 706
HhaI GCGC 2 cut(s) 670, 1199
Hin1I GRCGYC 1 cut(s) 717
Hin6I GCGC 2 cut(s) 668, 1197
HinP1I GCGC 2 cut(s) 668, 1197
HincII GTYRAC 2 cut(s) 2049, 3568
HindII GTYRAC 2 cut(s) 2049, 3568
HindIII AAGCTT 3 cut(s) 1923, 1985, 3366
HpaII CCGG 6 cut(s) 924, 1200, 2456, 3402, 3455, 3552
HphI GGTGA 6 cut(s) 748, 1525, 1666, 2723, 3607, 4084
Hpy166II GTNNAC 7 cut(s) 109, 703, 1205, 2049, 2341, 2388, 3568
Hpy8I GTNNAC 7 cut(s) 109, 703, 1205, 2049, 2341, 2388, 3568
Hpy99I CGWCG 2 cut(s) 722, 2961
HpyCH4III ACNGT 8 cut(s) 565, 606, 2046, 2169, 2317, 2549, 2787, 3067
HpyCH4IV ACGT 4 cut(s) 836, 1578, 2956, 3339
HpyF10VI GCNNNNNNNGC 5 cut(s) 632, 1220, 1517, 3656, 3668
HpySE526I ACGT 4 cut(s) 836, 1578, 2956, 3339
Hsp92I GRCGYC 1 cut(s) 717
HspAI GCGC 2 cut(s) 668, 1197
Kpn2I TCCGGA 2 cut(s) 2455, 3401
LguI GCTCTTC 2 cut(s) 1058, 3146
LmnI GCTCC 7 cut(s) 70, 749, 1113, 2956, 3297, 3404, 3824
MaeI CTAG 5 cut(s) 755, 1533, 2076, 2361, 2769
MaeII ACGT 4 cut(s) 836, 1578, 2956, 3339
MaeIII GTNAC 4 cut(s) 2525, 2715, 3829, 4060
MbiI CCGCTC 1 cut(s) 3399
MfeI CAATTG 2 cut(s) 1425, 3050
MflI RGATCY 8 cut(s) 373, 927, 1151, 1861, 2411, 2726, 3169, 3835
MhlI GDGCHC 2 cut(s) 754, 1237
MlsI TGGCCA 1 cut(s) 1684
MluNI TGGCCA 1 cut(s) 1684
MlyI GAGTC 5 cut(s) 708, 1538, 1541, 2413, 3377
MmeI TCCRAC 4 cut(s) 633, 700, 727, 3827
Mox20I TGGCCA 1 cut(s) 1684
Mph1103I ATGCAT 2 cut(s) 1337, 1596
MroI TCCGGA 2 cut(s) 2455, 3401
MroXI GAANNNNTTC 2 cut(s) 3958, 4079
MscI TGGCCA 1 cut(s) 1684
MslI CAYNNNNRTG 2 cut(s) 2685, 4067
Msp20I TGGCCA 1 cut(s) 1684
MspA1I CMGCKG 3 cut(s) 1511, 1645, 3354
MspI CCGG 6 cut(s) 924, 1200, 2456, 3402, 3455, 3552
MspR9I CCNGG 8 cut(s) 925, 2104, 2684, 2724, 3008, 3552, 3553, 3920
MssI GTTTAAAC 1 cut(s) 601
MunI CAATTG 2 cut(s) 1425, 3050
Mva1269I GAATGC 5 cut(s) 670, 1144, 1199, 2623, 3604
MvaI CCWGG 5 cut(s) 2104, 2684, 2724, 3008, 3920
MwoI GCNNNNNNNGC 5 cut(s) 632, 1220, 1517, 3656, 3668
NciI CCSGG 3 cut(s) 925, 3552, 3553
NdeI CATATG 1 cut(s) 2936
NlaIV GGNNCC 6 cut(s) 375, 929, 1235, 2378, 2449, 3299
NmeAIII GCCGAG 1 cut(s) 1255
NmuCI GTSAC 2 cut(s) 3829, 4060
NsiI ATGCAT 2 cut(s) 1337, 1596
NspI RCATGY 3 cut(s) 2176, 2631, 2855
NspV TTCGAA 1 cut(s) 408
PaeR7I CTCGAG 1 cut(s) 3385
PagI TCATGA 1 cut(s) 1059
PciI ACATGT 2 cut(s) 2627, 2851
PciSI GCTCTTC 2 cut(s) 1058, 3146
PcsI WCGNNNNNNNCGW 1 cut(s) 3993
PctI GAATGC 5 cut(s) 670, 1144, 1199, 2623, 3604
PdmI GAANNNNTTC 2 cut(s) 3958, 4079
PflMI CCANNNNNTGG 1 cut(s) 1759
PfoI TCCNGGA 1 cut(s) 2102
PleI GAGTC 5 cut(s) 707, 1537, 1541, 2413, 3377
PmeI GTTTAAAC 1 cut(s) 601
PpsI GAGTC 5 cut(s) 707, 1537, 1541, 2413, 3377
Ppu21I YACGTR 1 cut(s) 3340
PscI ACATGT 2 cut(s) 2627, 2851
PsiI TTATAA 2 cut(s) 525, 2505
Psp6I CCWGG 5 cut(s) 2102, 2682, 2722, 3006, 3918
PspFI CCCAGC 2 cut(s) 850, 3354
PspGI CCWGG 5 cut(s) 2102, 2682, 2722, 3006, 3918
PspN4I GGNNCC 6 cut(s) 375, 929, 1235, 2378, 2449, 3299
PspOMI GGGCCC 1 cut(s) 1233
PspPI GGNCC 5 cut(s) 1233, 1234, 1519, 2377, 2945
PstI CTGCAG 1 cut(s) 3775
PsuI RGATCY 8 cut(s) 373, 927, 1151, 1861, 2411, 2726, 3169, 3835
PvuII CAGCTG 1 cut(s) 3354
RsaI GTAC 7 cut(s) 387, 1641, 2171, 2431, 2748, 2784, 3185
RsaNI GTAC 7 cut(s) 386, 1640, 2170, 2430, 2747, 2783, 3184
RseI CAYNNNNRTG 2 cut(s) 2685, 4067
SalI GTCGAC 1 cut(s) 3566
SapI GCTCTTC 2 cut(s) 1058, 3146
Sau96I GGNCC 5 cut(s) 1233, 1234, 1519, 2377, 2945
ScaI AGTACT 2 cut(s) 2431, 2748
SchI GAGTC 5 cut(s) 708, 1538, 1541, 2413, 3377
ScrFI CCNGG 8 cut(s) 925, 2104, 2684, 2724, 3008, 3552, 3553, 3920
SduI GDGCHC 2 cut(s) 754, 1237
SfcI CTRYAG 2 cut(s) 795, 3771
Sfr274I CTCGAG 1 cut(s) 3385
SfuI TTCGAA 1 cut(s) 408
SinI GGWCC 2 cut(s) 2377, 2945
SlaI CTCGAG 1 cut(s) 3385
SmaI CCCGGG 1 cut(s) 3553
SmiMI CAYNNNNRTG 2 cut(s) 2685, 4067
SmlI CTYRAG 3 cut(s) 593, 1988, 3385
SmoI CTYRAG 3 cut(s) 593, 1988, 3385
SsiI CCGC 6 cut(s) 1511, 1645, 2734, 3397, 3467, 3602
SspI AATATT 2 cut(s) 2233, 3819
SspMI CTAG 5 cut(s) 755, 1533, 2076, 2361, 2769
StyD4I CCNGG 8 cut(s) 923, 2102, 2682, 2722, 3006, 3550, 3551, 3918
StyI CCWWGG 1 cut(s) 3940
TaaI ACNGT 8 cut(s) 565, 606, 2046, 2169, 2317, 2549, 2787, 3067
TaiI ACGT 4 cut(s) 839, 1581, 2959, 3342
TatI WGTACW 6 cut(s) 1639, 2169, 2429, 2746, 2782, 3183
TscAI CASTG 8 cut(s) 473, 522, 1879, 2768, 3044, 3834, 3853, 4043
TseFI GTSAC 2 cut(s) 3829, 4060
Tsp45I GTSAC 2 cut(s) 3829, 4060
TspGWI ACGGA 2 cut(s) 627, 720
TspMI CCCGGG 1 cut(s) 3551
TspRI CASTG 8 cut(s) 473, 522, 1879, 2768, 3044, 3834, 3853, 4043
Van91I CCANNNNNTGG 1 cut(s) 1759
VpaK11BI GGWCC 2 cut(s) 2377, 2945
XceI RCATGY 3 cut(s) 2176, 2631, 2855
XcmI CCANNNNNNNNNTGG 1 cut(s) 295
XhoI CTCGAG 1 cut(s) 3385
XmaI CCCGGG 1 cut(s) 3551
XmiI GTMKAC 4 cut(s) 108, 702, 1204, 3567
XmnI GAANNNNTTC 2 cut(s) 3958, 4079
XspI CTAG 5 cut(s) 755, 1533, 2076, 2361, 2769
ZrmI AGTACT 2 cut(s) 2431, 2748
Zsp2I ATGCAT 2 cut(s) 1337, 1596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.