Rw3G023020

disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000085
Physical Location & Seq
Forward (+)
28664607 .. 28667405
2799 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G023020.1

Sequence Viewer

Length: 2799 bp
ATGTCGAGCAGCAATTTTGAAGCATTTGAATCAACAAGAAAAGCCATGAATGAGCTGATGGAGGCATTAGAAGATGATGAGGTCGCTGCCACTGGGGTCTATGGAATGGGAGGGGTTGGGAAGACAACCATGGTGAAACATGTCGGTGCACATGCCCAAAAAATGGGGCTTTTTGATCGTGTGATTATGGCTGTTGTATCCCAAACCCCAGATCCAAGAAATATTCAAGGCGCACTTGCGGATCTGCTGGGCTTGACATTGGTGGAGGAGTCAGAAAGTGGACGAGCCGATAGATTGCAGAAGGAGATACTTGGAAGAAATAAGATCCTTATAATCTTAGACGACATTTGGAATACACTTGACTTGTCAAGCATTGGAATTCCCAGCTACAACGAGCTTCAAATCTGCAATTCCAAACTGCTATTCACCACAAGGAGATTGAATGTTTGCCATACCATGGAGAGCCAAGCAAAAATTCATCTCAATATCTTATCAGAAGATGATGCTTGGAACTTGTTTTTGAAGAAGGCAAAAAGGTCTATTCAAAAGTCTACCCCTTTCTATGAAGTAGCCAGAAAGGTAGCTAGAGAATGTGCTGGTCTTCCAGTTGCATTGATTGCAGTTGCGAGGGCACTTGGAGATAAAGAGCTTGAAGACTGGAAAGAAGCAGCTCGACAACTTGAGATGTCTCAACCTGCCAACCTTGATGAGGAGAAAGATGTGTTCAAGTGCATTAAGTTAAGCTATGATTACCTTGACAGTGACGACGCTAAGTCTTGCTTCTTCCTTTGCTGCCTATTCCCAGAAGATTATGACATCCGTGTTGAAGACTTGATGAGGTATGGGTTCGGAACAGGGTTGTTTCGACATGTCAACAGCACAATAGAATATGCCAGAGACAGAGCACATTCGGTTACTAGATACCTTAAAGCTTCTTGTCTGCTTTTGGATGGGACAAAAGAAGGATATGTAAGGATGCATGATGTCATTCGAGATATGGCCATATCCATTGCATTCTCTGTAGATGGCCACGCCTCTTTGGTGAAAACTAATTGTCATTTGAAAGATTGGTCGATGGATGCGGACGAAGGCTACTCAGCAATCTCACTAATCGAGAGCAACATTTGCAAGCTTCCAAACAAGTTGGTATGTTCAAAACTTCAGATTTTATTGCTACAAAACAATGCTGATATAAGAGAGATCCCAATAACCTTTTTTCAAAGCCCCAATGATTTAAGGGTCTTAGATCTTAGGATCACTGACATTTCATCACTGCCCCCATCATTCAGTCTCCTAACCAGACTCCAAGCTTTGCACTTAGATGATTGCTATTTCATGATTGACATATCCATACTTGGAAAACTGAAAAACCTTGAGATTCTTAGTATGAGACGCTGTTACCGTATGAATTTTCCAAAAGAGATTGGAAATTTGACGAATTTAAGAATGCTGGACTTGACTGGAACCTATTTTGAGACAGTTCCCTCTCAAGTGATACCAAGGTTGTATAGATTGGAAGTACTGTACATGAGATGTTCTTTTGGCAAGTGGGGTCGTAAAGCTGCGGGAGGCTGCTTGTGTTTTCTTTGTAAGAATGCTAGCTTTGATGAGTTAATTGGCTTGTCAAGTTTAAACACTTTGCAGGTTACCGTATGTGGTGCAGAACACTTGCCTCAAAACGTTGAGTTCAATCCAAATTGGGACAGGTTTTGTATAATTATCCAAAGATACAACACGGAAGATTTTGAATATCTTGACGAGGTCTTCCCACATCTTTCTTCAAGAACATTGATCACGGACGAAACCATGAATAACTTACCCGACTGGTTCATCAGTGTAGTGACAGAAAGAACAGATGATTTACGGTACACAGGTCGCCATGGCTTGACTAACCTTCTCGAGGAACACGACCGTGGGAGATTACATGGACTCAAGTATTTGTGTATAGAATCTTCCGAATATGCCAAAGTGTTGTTGAACACAATAACTTGGACTAGGGTGTCAAATGGACCTGTGTTTGAGAACTTGGAAGAGTTGGAAATATCCCTGGAGCTCTTGGAGGAGCTATGTGTCGGTGAATTACCACCTGGCTCTCTACGTAATCTTAAGAAATTGAAGATGGGTGGGTGTGATTGGTTGGTGAAACCGCTTTTACCGTCAAAGTTTTTGCACAGATTAAAGAGTCTGGAAGAACTAAGTTGTTCTCGTATGCGGGCAATGAAATATGTGTTTGGATTTGAAGTGCATGAACCACAACACGTTGTCTTGACGAGGCTGAGAATGATTTCATTGACAGGCATGGACAGTTTAATAAGCATATGGGATGGTCCTGCTCCATCTGGAATCTTCCAGAGTCTCAAAATCTTTTCCTTGGTTAAGTGCCAGAAACTGGAATATCTCTTCACATCTGATGTAGCTCGGTGTCTTTTGCAATTGGAAGACGTTTGGGTAGAGGAATGCGAATGCTTGGACAGAATAATTGAAGCGAGTGAGGAAATAGAGAGCAACAAGATCGTTCTTCCACAGTTGAAGAACTTGGCATTAAAACGTCTTCCAAAGCTCACAAGGCTGTCCAGTACAAGCAGCAGTACTATTGATATTGAAATCGAGTGCCCTACATTGGAACACTTGTATGTCTCTGAGTGCCCCCAGTTATCAATCTCTGCTTCTGATTTCAACAGCAGCAACGAAGTCCAACTCGATGATTACCAGCATTGGGGCACACTACGGTATAGGATCGACGACGTGAGTTACCGCTCAATTTTACTACGTGCGATAAGGTATGTCTCCATGTTATAA

Protein Analysis

932

Amino Acids

106.21

Weight (kDa)

5.45

Isoelectric Point (pI)

44.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 14 - 178 1.6e-30 NB-ARC domain
LRR_8 PF13855 386 - 443 5.5e-07 Leucine rich repeat
LRR_14 PF23598 448 - 706 1.1e-07 Leucine-rich repeat region
LRR_RPS2 PF23247 676 - 821 3.1e-24 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 332, 2797
AasI GACNNNNNNGTC 1 cut(s) 1999
Acc36I ACCTGC 2 cut(s) 703, 1633
AccB7I CCANNNNNTGG 3 cut(s) 163, 457, 2389
AccBSI CCGCTC 1 cut(s) 2757
AccI GTMKAC 1 cut(s) 551
AciI CCGC 6 cut(s) 239, 1082, 1565, 2147, 2212, 2755
AclI AACGTT 1 cut(s) 1680
AclWI GGATC 6 cut(s) 206, 249, 319, 1195, 1262, 2744
AcoI YGGCCR 2 cut(s) 999, 1027
AcsI RAATTY 5 cut(s) 378, 474, 1408, 1429, 1438
AcuI CTGAAG 1 cut(s) 1145
AfaI GTAC 5 cut(s) 1521, 1526, 1868, 2578, 2590
AfiI CCNNNNNNNGG 7 cut(s) 163, 457, 709, 1900, 2389, 2620, 2716
AflII CTTAAG 1 cut(s) 2105
AflIII ACRYGT 3 cut(s) 139, 868, 2257
AhdI GACNNNNNGTC 1 cut(s) 772
AjiI CACGTC 1 cut(s) 2746
AjnI CCWGG 2 cut(s) 2046, 2086
AjuI GAANNNNNNNTTGG 4 cut(s) 407, 439, 459, 491
AleI CACNNNNGTG 1 cut(s) 1911
Alw21I GWGCWC 3 cut(s) 151, 907, 2055
Alw26I GTCTC 8 cut(s) 693, 891, 1295, 1384, 1469, 2360, 2641, 2791
Alw44I GTGCAC 1 cut(s) 147
AlwI GGATC 6 cut(s) 206, 249, 319, 1195, 1262, 2744
AlwNI CAGNNNCTG 1 cut(s) 2389
Ama87I CYCGRG 1 cut(s) 1898
AoxI GGCC 2 cut(s) 999, 1027
ApaLI GTGCAC 1 cut(s) 147
ApeKI GCWGC 8 cut(s) 9, 86, 668, 792, 1562, 1572, 2583, 2682
ApoI RAATTY 5 cut(s) 378, 474, 1408, 1429, 1438
Asp700I GAANNNNTTC 1 cut(s) 2284
AspLEI GCGC 1 cut(s) 233
AspS9I GGNCC 2 cut(s) 2009, 2327
AsuHPI GGTGA 5 cut(s) 145, 418, 1054, 2087, 2152
AsuNHI GCTAGC 1 cut(s) 1598
AvaI CYCGRG 1 cut(s) 1898
AvaII GGWCC 2 cut(s) 2009, 2327
BaeGI GKGCMC 5 cut(s) 151, 634, 2615, 2648, 2723
BaeI ACNNNNGTAYC 4 cut(s) 1487, 1520, 1858, 1891
BalI TGGCCA 2 cut(s) 1001, 1029
BanII GRGCYC 1 cut(s) 2055
BarI GAAGNNNNNNTAC 2 cut(s) 1936, 1968
BbsI GAAGAC 7 cut(s) 128, 593, 660, 834, 1756, 2445, 2543
Bbv12I GWGCWC 3 cut(s) 151, 907, 2055
BbvI GCAGC 8 cut(s) 21, 73, 680, 779, 1549, 1559, 2595, 2694
BccI CCATC 8 cut(s) 52, 944, 1019, 1069, 1288, 2113, 2318, 2344
BcgI CGANNNNNNTGC 2 cut(s) 2494, 2528
BciT130I CCWGG 2 cut(s) 2048, 2088
BciVI GTATCC 1 cut(s) 208
BclI TGATCA 1 cut(s) 1791
BcoDI GTCTC 8 cut(s) 693, 891, 1295, 1384, 1469, 2360, 2641, 2791
BfaI CTAG 4 cut(s) 585, 918, 1599, 1995
BfmI CTRYAG 1 cut(s) 1020
BfrI CTTAAG 1 cut(s) 2105
BfuAI ACCTGC 2 cut(s) 703, 1633
BfuI GTATCC 1 cut(s) 208
BglII AGATCT 1 cut(s) 1246
BisI GCNGC 8 cut(s) 10, 87, 669, 793, 1563, 1573, 2584, 2683
BlsI GCNGC 8 cut(s) 11, 88, 670, 794, 1564, 1574, 2585, 2684
BmcAI AGTACT 2 cut(s) 1521, 2590
Bme1390I CCNGG 2 cut(s) 2048, 2088
Bme18I GGWCC 2 cut(s) 2009, 2327
BmeRI GACNNNNNGTC 1 cut(s) 772
BmeT110I CYCGRG 1 cut(s) 1898
BmgBI CACGTC 1 cut(s) 2746
BmgT120I GGNCC 2 cut(s) 2009, 2327
BmiI GGNNCC 1 cut(s) 1465
BmrFI CCNGG 2 cut(s) 2048, 2088
BmrI ACTGGG 2 cut(s) 102, 2644
BmsI GCATC 3 cut(s) 493, 966, 1069
BmtI GCTAGC 1 cut(s) 1602
BmuI ACTGGG 2 cut(s) 102, 2644
BpiI GAAGAC 7 cut(s) 128, 593, 660, 834, 1756, 2445, 2543
BpmI CTGGAG 1 cut(s) 2069
BpuEI CTTGAG 4 cut(s) 701, 1394, 1473, 1916
BsaAI YACGTR 2 cut(s) 2099, 2771
BsaJI CCNNGG 7 cut(s) 129, 456, 1499, 1879, 1912, 2046, 2370
Bsc4I CCNNNNNNNGG 7 cut(s) 163, 457, 709, 1900, 2389, 2620, 2716
Bse1I ACTGG 8 cut(s) 97, 605, 662, 1465, 1829, 2394, 2574, 2650
Bse3DI GCAATG 2 cut(s) 1008, 2223
BseBI CCWGG 2 cut(s) 2048, 2088
BseDI CCNNGG 7 cut(s) 129, 456, 1499, 1879, 1912, 2046, 2370
BseGI GGATG 5 cut(s) 816, 955, 981, 1084, 2329
BseLI CCNNNNNNNGG 7 cut(s) 163, 457, 709, 1900, 2389, 2620, 2716
BseMI GCAATG 2 cut(s) 1008, 2223
BseMII CTCAG 3 cut(s) 1110, 2267, 2631
BseNI ACTGG 8 cut(s) 97, 605, 662, 1465, 1829, 2394, 2574, 2650
BseRI GAGGAG 3 cut(s) 281, 725, 2075
BseSI GKGCMC 5 cut(s) 151, 634, 2615, 2648, 2723
BseXI GCAGC 8 cut(s) 21, 73, 680, 779, 1549, 1559, 2595, 2694
BseYI CCCAGC 2 cut(s) 247, 383
BsgI GTGCAG 1 cut(s) 1680
Bsh1285I CGRYCG 1 cut(s) 1912
BshFI GGCC 2 cut(s) 1001, 1029
BsiEI CGRYCG 1 cut(s) 1912
BsiHKAI GWGCWC 3 cut(s) 151, 907, 2055
BsiHKCI CYCGRG 1 cut(s) 1898
BslFI GGGAC 2 cut(s) 967, 1715
BslI CCNNNNNNNGG 7 cut(s) 163, 457, 709, 1900, 2389, 2620, 2716
BsmAI GTCTC 8 cut(s) 693, 891, 1295, 1384, 1469, 2360, 2641, 2791
BsmBI CGTCTC 1 cut(s) 1384
BsmFI GGGAC 2 cut(s) 967, 1715
BsmI GAATGC 5 cut(s) 1013, 1452, 1600, 2462, 2468
BsnI GGCC 2 cut(s) 1001, 1029
BsoBI CYCGRG 1 cut(s) 1898
Bsp1286I GDGCHC 7 cut(s) 151, 634, 907, 2055, 2615, 2648, 2723
Bsp1407I TGTACA 1 cut(s) 1524
Bsp19I CCATGG 3 cut(s) 129, 456, 1879
BspACI CCGC 6 cut(s) 239, 1082, 1565, 2147, 2212, 2755
BspANI GGCC 2 cut(s) 1001, 1029
BspCNI CTCAG 3 cut(s) 1109, 2268, 2632
BspHI TCATGA 1 cut(s) 1335
BspLI GGNNCC 1 cut(s) 1465
BspMI ACCTGC 2 cut(s) 703, 1633
BspOI GCTAGC 1 cut(s) 1602
BspPI GGATC 6 cut(s) 206, 249, 319, 1195, 1262, 2744
BspTI CTTAAG 1 cut(s) 2105
BsrBI CCGCTC 1 cut(s) 2757
BsrDI GCAATG 2 cut(s) 1008, 2223
BsrGI TGTACA 1 cut(s) 1524
BsrI ACTGG 8 cut(s) 97, 605, 662, 1465, 1829, 2394, 2574, 2650
BssECI CCNNGG 7 cut(s) 129, 456, 1499, 1879, 1912, 2046, 2370
BssT1I CCWWGG 5 cut(s) 129, 456, 1499, 1879, 2370
Bst2UI CCWGG 2 cut(s) 2048, 2088
Bst6I CTCTTC 2 cut(s) 2025, 2405
BstAFI CTTAAG 1 cut(s) 2105
BstAPI GCANNNNNTGC 2 cut(s) 617, 1125
BstAUI TGTACA 1 cut(s) 1524
BstBAI YACGTR 2 cut(s) 2099, 2771
BstC8I GCNNGC 3 cut(s) 1130, 1600, 2214
BstDSI CCRYGG 4 cut(s) 129, 456, 1879, 1912
BstEII GGTNACC 1 cut(s) 1645
BstENI CCTNNNNNAGG 2 cut(s) 707, 1898
BstF5I GGATG 5 cut(s) 816, 955, 981, 1084, 2329
BstHHI GCGC 1 cut(s) 233
BstMAI GTCTC 8 cut(s) 693, 891, 1295, 1384, 1469, 2360, 2641, 2791
BstMCI CGRYCG 1 cut(s) 1912
BstMWI GCNNNNNNNGC 3 cut(s) 617, 1125, 2566
BstNI CCWGG 2 cut(s) 2048, 2088
BstNSI RCATGY 3 cut(s) 143, 155, 872
BstPI GGTNACC 1 cut(s) 1645
BstSCI CCNGG 2 cut(s) 2046, 2086
BstSFI CTRYAG 1 cut(s) 1020
BstSLI GKGCMC 5 cut(s) 151, 634, 2615, 2648, 2723
BstSNI TACGTA 1 cut(s) 2099
BstV1I GCAGC 8 cut(s) 21, 73, 680, 779, 1549, 1559, 2595, 2694
BstV2I GAAGAC 7 cut(s) 128, 593, 660, 834, 1756, 2445, 2543
BstX2I RGATCY 5 cut(s) 211, 241, 324, 1200, 1246
BstYI RGATCY 5 cut(s) 211, 241, 324, 1200, 1246
BsuI GTATCC 1 cut(s) 208
BsuRI GGCC 2 cut(s) 1001, 1029
BtgI CCRYGG 4 cut(s) 129, 456, 1879, 1912
BtrI CACGTC 1 cut(s) 2746
BtsCI GGATG 5 cut(s) 816, 955, 981, 1084, 2329
BtsI GCAGTG 1 cut(s) 1271
BtsIMutI CAGTG 5 cut(s) 90, 766, 1257, 1271, 1840
BveI ACCTGC 2 cut(s) 703, 1633
Cac8I GCNNGC 3 cut(s) 1130, 1600, 2214
CaiI CAGNNNCTG 1 cut(s) 2389
CciI TCATGA 1 cut(s) 1335
CfoI GCGC 1 cut(s) 233
Cfr13I GGNCC 2 cut(s) 2009, 2327
CseI GACGC 2 cut(s) 776, 1401
Csp6I GTAC 5 cut(s) 1520, 1525, 1867, 2577, 2589
CviQI GTAC 5 cut(s) 1520, 1525, 1867, 2577, 2589
DraI TTTAAA 1 cut(s) 1632
DrdI GACNNNNNNGTC 1 cut(s) 1999
DriI GACNNNNNGTC 1 cut(s) 772
DseDI GACNNNNNNGTC 1 cut(s) 1999
EaeI YGGCCR 2 cut(s) 999, 1027
Eam1104I CTCTTC 2 cut(s) 2025, 2405
Eam1105I GACNNNNNGTC 1 cut(s) 772
EarI CTCTTC 2 cut(s) 2025, 2405
Ecl136II GAGCTC 1 cut(s) 2053
Eco105I TACGTA 1 cut(s) 2099
Eco130I CCWWGG 5 cut(s) 129, 456, 1499, 1879, 2370
Eco24I GRGCYC 1 cut(s) 2055
Eco47I GGWCC 2 cut(s) 2009, 2327
Eco53kI GAGCTC 1 cut(s) 2053
Eco57I CTGAAG 1 cut(s) 1145
Eco88I CYCGRG 1 cut(s) 1898
Eco91I GGTNACC 1 cut(s) 1645
EcoICRI GAGCTC 1 cut(s) 2053
EcoNI CCTNNNNNAGG 2 cut(s) 707, 1898
EcoO65I GGTNACC 1 cut(s) 1645
EcoRI GAATTC 1 cut(s) 378
EcoRII CCWGG 2 cut(s) 2046, 2086
EcoT14I CCWWGG 5 cut(s) 129, 456, 1499, 1879, 2370
EcoT22I ATGCAT 1 cut(s) 981
EcoT38I GRGCYC 1 cut(s) 2055
ErhI CCWWGG 5 cut(s) 129, 456, 1499, 1879, 2370
Esp3I CGTCTC 1 cut(s) 1384
FalI AAGNNNNNCTT 4 cut(s) 219, 251, 764, 796
FaqI GGGAC 2 cut(s) 967, 1715
FauI CCCGC 2 cut(s) 1558, 2205
FauNDI CATATG 1 cut(s) 2318
FbaI TGATCA 1 cut(s) 1791
FblI GTMKAC 1 cut(s) 551
Fnu4HI GCNGC 8 cut(s) 10, 87, 669, 793, 1563, 1573, 2584, 2683
FokI GGATG 5 cut(s) 803, 962, 988, 1091, 2336
FriOI GRGCYC 1 cut(s) 2055
Fsp4HI GCNGC 8 cut(s) 10, 87, 669, 793, 1563, 1573, 2584, 2683
FspBI CTAG 4 cut(s) 585, 918, 1599, 1995
GlaI GCGC 1 cut(s) 232
GluI GCNGC 8 cut(s) 10, 87, 669, 793, 1563, 1573, 2584, 2683
GsaI CCCAGC 2 cut(s) 251, 387
GsuI CTGGAG 1 cut(s) 2069
HaeIII GGCC 2 cut(s) 1001, 1029
HgaI GACGC 2 cut(s) 776, 1401
HhaI GCGC 1 cut(s) 233
Hin6I GCGC 1 cut(s) 231
HinP1I GCGC 1 cut(s) 231
HincII GTYRAC 1 cut(s) 874
HindII GTYRAC 1 cut(s) 874
HindIII AAGCTT 3 cut(s) 930, 1130, 1308
HinfI GANTC 9 cut(s) 29, 269, 1302, 1378, 1929, 1949, 2182, 2343, 2353
HphI GGTGA 5 cut(s) 145, 418, 1054, 2087, 2152
Hpy166II GTNNAC 5 cut(s) 149, 281, 552, 874, 1869
Hpy188I TCNGA 8 cut(s) 274, 496, 851, 1164, 1957, 2410, 2641, 2671
Hpy8I GTNNAC 5 cut(s) 149, 281, 552, 874, 1869
Hpy99I CGWCG 3 cut(s) 770, 2744, 2747
HpyAV CCTTC 5 cut(s) 295, 520, 956, 1082, 1904
HpyCH4IV ACGT 7 cut(s) 1680, 2098, 2259, 2442, 2548, 2745, 2770
HpyF10VI GCNNNNNNNGC 3 cut(s) 617, 1125, 2566
HpySE526I ACGT 7 cut(s) 1680, 2098, 2259, 2442, 2548, 2745, 2770
HspAI GCGC 1 cut(s) 231
Ksp22I TGATCA 1 cut(s) 1791
LmnI GCTCC 3 cut(s) 2050, 2062, 2338
Lsp1109I GCAGC 8 cut(s) 21, 73, 680, 779, 1549, 1559, 2595, 2694
LweI GCATC 3 cut(s) 493, 966, 1069
MaeI CTAG 4 cut(s) 585, 918, 1599, 1995
MaeII ACGT 7 cut(s) 1680, 2098, 2259, 2442, 2548, 2745, 2770
MaeIII GTNAC 6 cut(s) 761, 913, 1397, 1645, 1840, 2750
MbiI CCGCTC 1 cut(s) 2757
MfeI CAATTG 1 cut(s) 2432
MflI RGATCY 5 cut(s) 211, 241, 324, 1200, 1246
MhlI GDGCHC 7 cut(s) 151, 634, 907, 2055, 2615, 2648, 2723
MlsI TGGCCA 2 cut(s) 1001, 1029
MluNI TGGCCA 2 cut(s) 1001, 1029
MlyI GAGTC 5 cut(s) 278, 1296, 1923, 2191, 2362
MmeI TCCRAC 2 cut(s) 2016, 2719
Mox20I TGGCCA 2 cut(s) 1001, 1029
Mph1103I ATGCAT 1 cut(s) 981
MroXI GAANNNNTTC 1 cut(s) 2284
MscI TGGCCA 2 cut(s) 1001, 1029
MslI CAYNNNNRTG 4 cut(s) 144, 1320, 1911, 2631
Msp20I TGGCCA 2 cut(s) 1001, 1029
MspCI CTTAAG 1 cut(s) 2105
MspR9I CCNGG 2 cut(s) 2048, 2088
MssI GTTTAAAC 1 cut(s) 1632
MunI CAATTG 1 cut(s) 2432
Mva1269I GAATGC 5 cut(s) 1013, 1452, 1600, 2462, 2468
MvaI CCWGG 2 cut(s) 2048, 2088
MwoI GCNNNNNNNGC 3 cut(s) 617, 1125, 2566
NcoI CCATGG 3 cut(s) 129, 456, 1879
NdeI CATATG 1 cut(s) 2318
NheI GCTAGC 1 cut(s) 1598
NlaIV GGNNCC 1 cut(s) 1465
NmuCI GTSAC 2 cut(s) 761, 1840
NsiI ATGCAT 1 cut(s) 981
NspI RCATGY 3 cut(s) 143, 155, 872
OliI CACNNNNGTG 1 cut(s) 1911
PaeR7I CTCGAG 1 cut(s) 1898
PagI TCATGA 1 cut(s) 1335
PciI ACATGT 2 cut(s) 139, 868
PcsI WCGNNNNNNNCGW 2 cut(s) 1399, 1905
PctI GAATGC 5 cut(s) 1013, 1452, 1600, 2462, 2468
PdmI GAANNNNTTC 1 cut(s) 2284
PfeI GAWTC 4 cut(s) 29, 1378, 1949, 2343
PflFI GACNNNGTC 1 cut(s) 1760
PflMI CCANNNNNTGG 3 cut(s) 163, 457, 2389
PkrI GCNGC 8 cut(s) 11, 88, 670, 794, 1564, 1574, 2585, 2684
PleI GAGTC 5 cut(s) 277, 1296, 1923, 2190, 2361
PmeI GTTTAAAC 1 cut(s) 1632
PpsI GAGTC 5 cut(s) 277, 1296, 1923, 2190, 2361
Ppu21I YACGTR 2 cut(s) 2099, 2771
PscI ACATGT 2 cut(s) 139, 868
PsiI TTATAA 2 cut(s) 332, 2797
Psp124BI GAGCTC 1 cut(s) 2055
Psp1406I AACGTT 1 cut(s) 1680
Psp6I CCWGG 2 cut(s) 2046, 2086
PspEI GGTNACC 1 cut(s) 1645
PspFI CCCAGC 2 cut(s) 247, 383
PspGI CCWGG 2 cut(s) 2046, 2086
PspN4I GGNNCC 1 cut(s) 1465
PspPI GGNCC 2 cut(s) 2009, 2327
PstNI CAGNNNCTG 1 cut(s) 2389
PsuI RGATCY 5 cut(s) 211, 241, 324, 1200, 1246
PsyI GACNNNGTC 1 cut(s) 1760
RsaI GTAC 5 cut(s) 1521, 1526, 1868, 2578, 2590
RsaNI GTAC 5 cut(s) 1520, 1525, 1867, 2577, 2589
RseI CAYNNNNRTG 4 cut(s) 144, 1320, 1911, 2631
SacI GAGCTC 1 cut(s) 2055
SatI GCNGC 8 cut(s) 10, 87, 669, 793, 1563, 1573, 2584, 2683
Sau96I GGNCC 2 cut(s) 2009, 2327
ScaI AGTACT 2 cut(s) 1521, 2590
SchI GAGTC 5 cut(s) 278, 1296, 1923, 2191, 2362
ScrFI CCNGG 2 cut(s) 2048, 2088
SduI GDGCHC 7 cut(s) 151, 634, 907, 2055, 2615, 2648, 2723
SfaNI GCATC 3 cut(s) 493, 966, 1069
SfcI CTRYAG 1 cut(s) 1020
Sfr274I CTCGAG 1 cut(s) 1898
SinI GGWCC 2 cut(s) 2009, 2327
SlaI CTCGAG 1 cut(s) 1898
SmiMI CAYNNNNRTG 4 cut(s) 144, 1320, 1911, 2631
SmlI CTYRAG 6 cut(s) 680, 1373, 1488, 1898, 1931, 2105
SmoI CTYRAG 6 cut(s) 680, 1373, 1488, 1898, 1931, 2105
SnaBI TACGTA 1 cut(s) 2099
SsiI CCGC 6 cut(s) 239, 1082, 1565, 2147, 2212, 2755
SspI AATATT 1 cut(s) 223
SspMI CTAG 4 cut(s) 585, 918, 1599, 1995
SstI GAGCTC 1 cut(s) 2055
StyD4I CCNGG 2 cut(s) 2046, 2086
StyI CCWWGG 5 cut(s) 129, 456, 1499, 1879, 2370
TaiI ACGT 7 cut(s) 1683, 2101, 2262, 2445, 2551, 2748, 2773
TatI WGTACW 4 cut(s) 1519, 1524, 2576, 2588
TfiI GAWTC 4 cut(s) 29, 1378, 1949, 2343
TscAI CASTG 5 cut(s) 97, 766, 1264, 1278, 1840
TseFI GTSAC 2 cut(s) 761, 1840
TseI GCWGC 8 cut(s) 9, 86, 668, 792, 1562, 1572, 2583, 2682
Tsp45I GTSAC 2 cut(s) 761, 1840
TspGWI ACGGA 3 cut(s) 809, 1751, 1811
TspRI CASTG 5 cut(s) 97, 766, 1264, 1278, 1840
Tth111I GACNNNGTC 1 cut(s) 1760
Van91I CCANNNNNTGG 3 cut(s) 163, 457, 2389
Vha464I CTTAAG 1 cut(s) 2105
VneI GTGCAC 1 cut(s) 147
VpaK11BI GGWCC 2 cut(s) 2009, 2327
XagI CCTNNNNNAGG 2 cut(s) 707, 1898
XapI RAATTY 5 cut(s) 378, 474, 1408, 1429, 1438
XceI RCATGY 3 cut(s) 143, 155, 872
XhoI CTCGAG 1 cut(s) 1898
XmiI GTMKAC 1 cut(s) 551
XmnI GAANNNNTTC 1 cut(s) 2284
XspI CTAG 4 cut(s) 585, 918, 1599, 1995
ZrmI AGTACT 2 cut(s) 1521, 2590
Zsp2I ATGCAT 1 cut(s) 981
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.