Rroxscaffold_6G00398280

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000085
Physical Location & Seq
Reverse (-)
20146717 .. 20155971
9255 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00398280.1

Sequence Viewer

Length: 2697 bp
ATGGGGCTTTTTGATCGTGTGATTATGGCTGTTGTATCCCAAACCCCAGATACAAGAAATATTCAAGGCGCACTTGCGGATCTGCTGGGCTTGAAATTAGTGGAGGAGTCAGAAAGTGGACGAGCCGATAGATTGCAGACGGAGATACTTGGAAGAAATAAGATCCTTATAATCTTAGATGACATTTGGAATACACTTGACTTGTCAAGCATTGGAATTCCCAGCTACAACGAGCTTCAAATCTGCAATTCCAAACTGCTGTTCACCACAAGGAGATCGAATGTTTGCCATACCATGGAGAGCCAAGCAAAAATTCATCTCAATATCTTATCAGAAGATGATGCTTGGAACTTGTTTTTGAAGAAGGCAAGAAGGTCTTTTCAAAAGTCTAGCCCTTTCTATGAAGTAGCCAGAAAGGTAGCTAGAGAATGTGCTGGTCTCCCAGTTGCATTGATTGCAGTTGCGAGGGCACTTGGAGATAAAGAGCTTGAAGACTGGAAAGAAGCAGCTCGACAACTTGAGATGTCTCAACCTGCCAACCTTGACGAGGAGAAAGATGTGTTCAAGTGCATTAAGTTGAGCTATGATTACCTTGACAGTGACGACGCCAAGTCTTGCTTCTTGCTTTGCTGCCTATTCCCAGAAGATTATGACATCCGTGTTGAAGACTTGATGAGCTATGGGTTCGGAACAGGGTTGTTTCGACATGTCAACAGCACAATAGAATATGCCAGAGTCAGAGCACATTCGGTCACTAGATACCTTAAAGCTTCTTGTCTGCTTTTGGATGGGACAAAAGAAGGATATGTAAGGATGCATGATGTCATTCGAGATACGGCCATATCCATTGCATTCTCTGTAGATGGCCACGCCTCTTTGGTGAAACCTAATTGTCATTTGAAAGATTGGTCGATGGATGCGGACGAAGGCTACTCAGCAATCTCACTAATCAAAAGCAACATTTGCAAGCTTCCCAACAAGTTGGTATGTTCAAAACTTCAGATTTTATTGCTACAGGAAAATGCTGCTATAAGAGAGATCCCAATAACCTTTTTTCAAAGCCCCAGTGATTTAAGGGTCTTAGATCTTAGCAACACTGGCATTTCATCACTGCCCCCATCATTCAGTCTCCTAACCAGACTCCAAGCTTTGCATTTAGATGATTGCGAGTCCATGATTGACATATCCCTACTTGGAAAACTGAAAAAGCTTGAGATTCTTAGTATGAGATGCTGTTGCTCTATTAAGAAATTTCCAAAAGTGATTGGAAATTTGACGAATTTAAGAATGTTGGACTTGACTGGAACCCGTTTTAAGACAGTTCCCTCTCAAGTGATATCAAGGTTGTATAGATTGGAAGTACTGTACATGGGATGTTATTTTGGCCAGTGGGGTTGTAAAGCTGCGGGAGGCTGCTTGTGTTTTCGTTGTAAGAATGCTAGCTTTGATGAGTTAATTGGCTTGTCAAGTTTAAACACTTTGAAGGTTGCCGTATGTGGTGCAGAATGCTTGCCACAAAACGTTGAGTTCAATCCAAATTGGGACAGGTTTTATATAATTATCCAAAGATACGGCCCGGACAATCTTGGCGATTTTGGTTTCAGATTCCCACATCGTTCTTCAAGAACATTAATCACGGTCGAAACCATGAATAACTTACCCGATTGGTTCATCAGTGTAGTGACAGAAAGAACAGATGATTTATGGTACAGAGGTCACCATGGCTTAACTAACCTTCTGGAGGAACACGACCGTGGGAGATTACATGGACTCAAGTATTTGCGTATACTATGTTCCGAATATGCCAAAGTGTTGTTGAACACAATAACATGGACTAGGGTGTCAAATGAACCTGTGTTTGAGAACTTGGAAAAGTTGGAAATCGCCGGGTGTCTCTTGGAGGAGCTATGTGTCGGTGAATTACCACCTGGCTCTCTACGTAATCTTAAGAAATTGATGATGGGTGAGTGTGATTGGTTGGTGAAACCGCTTTTACCGTCAAAGTTGTTGCACAGATTAGAGAGTCTGGAAGAACTATGTTGTTCTTTTATGCGGGAAATGAAATATGTGTTTGGATTTGAAGGGCATGAACCACAACACGTTGTCTTGACGAGGCTGAGAATGATTTCATTGACATCCATGAACAGCTTAATAAACATATGGGATGGTCCTGCTCCATCTGGAATCTTCCAGAGTCTCAAAACCTTTTCCTTGGTTAATTGCCAGAAACTGGAATATCTCTTCACATCTGAGGTAGCTCGATGTCTTTTGCAATTGGAAGACGTTTTGGCAGAGGGTTGCGATAGCTTGGACAGAATAATTGAAGCGAGTGCGGAAATAGAGAGCAACAAGATCGTTCTTCCACAGTTGAAGAACTTGTTTTTAGAACGTCTTCCAAAGCTCACAAGGCTGTCCAGTACAAGCAGCAGTACTATTGATATTGAAATCGAGTGCCCTACATTGGAACACTTATATGTCTCTGCGTGCCCCCAGTTATCAATCTCTGCTTCTGATTTCAACAGCAGCAACGAAGTCCAACTAGATGATCACCAGCATTGGGGCACACTACGCTATAGGATCCACGACGTGAGTTACCGCTCACAGTTAGTAGTTGGGATCAGAACGCGCTTTAATTGGGCATGGCAGAAGGCGCTTATTAGGGGCATGGACTTGGATATTGGGGAGTATGCGGAATGA

Protein Analysis

898

Amino Acids

101.95

Weight (kDa)

5.94

Isoelectric Point (pI)

44.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 3 - 124 4.1e-19 NB-ARC domain
LRR_8 PF13855 332 - 389 2.5e-08 Leucine rich repeat
LRR_14 PF23598 357 - 412 1.4e-06 Leucine-rich repeat region
LRR_14 PF23598 391 - 458 8.2e-08 Leucine-rich repeat region
LRR_RPS2 PF23247 623 - 767 1.3e-21 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 170
AasI GACNNNNNNGTC 1 cut(s) 1840
Acc36I ACCTGC 1 cut(s) 541
AccB7I CCANNNNNTGG 2 cut(s) 295, 2230
AccBSI CCGCTC 1 cut(s) 2598
AccI GTMKAC 1 cut(s) 1786
AccII CGCG 1 cut(s) 2626
AciI CCGC 8 cut(s) 77, 920, 1406, 1988, 2053, 2333, 2596, 2690
AclI AACGTT 1 cut(s) 1521
AclWI GGATC 6 cut(s) 87, 157, 1033, 2572, 2585, 2624
AcoI YGGCCR 3 cut(s) 837, 865, 1384
AcsI RAATTY 5 cut(s) 216, 312, 1250, 1270, 1279
AcuI CTGAAG 1 cut(s) 983
AcyI GRCGYC 1 cut(s) 606
AdeI CACNNNGTG 1 cut(s) 2587
AfaI GTAC 5 cut(s) 1362, 1367, 1709, 2419, 2431
AfiI CCNNNNNNNGG 6 cut(s) 295, 547, 1741, 2230, 2461, 2557
AflII CTTAAG 1 cut(s) 1946
AflIII ACRYGT 2 cut(s) 706, 2098
AhdI GACNNNNNGTC 1 cut(s) 610
AjiI CACGTC 1 cut(s) 2587
AjnI CCWGG 1 cut(s) 1927
AjuI GAANNNNNNNTTGG 6 cut(s) 245, 277, 297, 329, 602, 634
AleI CACNNNNGTG 1 cut(s) 1752
Alw21I GWGCWC 1 cut(s) 745
Alw26I GTCTC 6 cut(s) 443, 531, 1133, 1898, 2201, 2482
AlwI GGATC 6 cut(s) 87, 157, 1033, 2572, 2585, 2624
AlwNI CAGNNNCTG 1 cut(s) 2230
AoxI GGCC 4 cut(s) 837, 865, 1384, 1573
ApeKI GCWGC 7 cut(s) 506, 630, 1025, 1403, 1413, 2424, 2523
ApoI RAATTY 5 cut(s) 216, 312, 1250, 1270, 1279
AseI ATTAAT 1 cut(s) 1631
Asp700I GAANNNNTTC 2 cut(s) 2125, 2391
AspLEI GCGC 3 cut(s) 71, 2628, 2653
AspS9I GGNCC 2 cut(s) 1574, 2168
AsuC2I CCSGG 2 cut(s) 1577, 1888
AsuHPI GGTGA 7 cut(s) 256, 892, 1709, 1928, 1976, 1993, 2540
AsuNHI GCTAGC 1 cut(s) 1439
AvaII GGWCC 1 cut(s) 2168
BaeGI GKGCMC 4 cut(s) 472, 2456, 2489, 2564
BaeI ACNNNNGTAYC 2 cut(s) 1699, 1732
BalI TGGCCA 2 cut(s) 867, 1386
BamHI GGATCC 1 cut(s) 2577
BbsI GAAGAC 4 cut(s) 498, 672, 2286, 2384
Bbv12I GWGCWC 1 cut(s) 745
BbvI GCAGC 7 cut(s) 518, 617, 1012, 1390, 1400, 2436, 2535
BccI CCATC 7 cut(s) 782, 857, 907, 1126, 1954, 2159, 2185
BceAI ACGGC 3 cut(s) 852, 1475, 1588
BcgI CGANNNNNNTGC 2 cut(s) 2335, 2369
BciT130I CCWGG 1 cut(s) 1929
BciVI GTATCC 1 cut(s) 46
BclI TGATCA 1 cut(s) 2545
BcnI CCSGG 2 cut(s) 1577, 1888
BcoDI GTCTC 6 cut(s) 443, 531, 1133, 1898, 2201, 2482
BfaI CTAG 6 cut(s) 390, 423, 756, 1440, 1836, 2540
BfmI CTRYAG 3 cut(s) 858, 1013, 2572
BfoI RGCGCY 1 cut(s) 2654
BfrI CTTAAG 1 cut(s) 1946
BfuAI ACCTGC 1 cut(s) 541
BfuI GTATCC 1 cut(s) 46
BglII AGATCT 1 cut(s) 1084
BisI GCNGC 7 cut(s) 507, 631, 1026, 1404, 1414, 2425, 2524
BlsI GCNGC 7 cut(s) 508, 632, 1027, 1405, 1415, 2426, 2525
BmcAI AGTACT 2 cut(s) 1362, 2431
Bme1390I CCNGG 3 cut(s) 1577, 1888, 1929
Bme18I GGWCC 1 cut(s) 2168
BmeRI GACNNNNNGTC 1 cut(s) 610
BmgBI CACGTC 1 cut(s) 2587
BmgT120I GGNCC 2 cut(s) 1574, 2168
BmiI GGNNCC 2 cut(s) 1306, 2579
BmrFI CCNGG 3 cut(s) 1577, 1888, 1929
BmrI ACTGGG 3 cut(s) 437, 1059, 2485
BmsI GCATC 4 cut(s) 331, 804, 907, 1220
BmtI GCTAGC 1 cut(s) 1443
BmuI ACTGGG 3 cut(s) 437, 1059, 2485
BpiI GAAGAC 4 cut(s) 498, 672, 2286, 2384
BpmI CTGGAG 1 cut(s) 1760
BpuEI CTTGAG 4 cut(s) 539, 1232, 1314, 1757
BpuMI CCSGG 2 cut(s) 1577, 1888
BsaAI YACGTR 1 cut(s) 1940
BsaHI GRCGYC 1 cut(s) 606
BsaI GGTCTC 1 cut(s) 443
BsaJI CCNNGG 4 cut(s) 294, 1720, 1753, 2211
Bsc4I CCNNNNNNNGG 6 cut(s) 295, 547, 1741, 2230, 2461, 2557
Bse1I ACTGG 9 cut(s) 443, 500, 1065, 1102, 1306, 1387, 2235, 2415, 2491
Bse3DI GCAATG 1 cut(s) 846
BseBI CCWGG 1 cut(s) 1929
BseDI CCNNGG 4 cut(s) 294, 1720, 1753, 2211
BseGI GGATG 7 cut(s) 654, 793, 819, 922, 1379, 2135, 2170
BseLI CCNNNNNNNGG 6 cut(s) 295, 547, 1741, 2230, 2461, 2557
BseMI GCAATG 1 cut(s) 846
BseMII CTCAG 3 cut(s) 948, 2108, 2241
BseNI ACTGG 9 cut(s) 443, 500, 1065, 1102, 1306, 1387, 2235, 2415, 2491
BseRI GAGGAG 3 cut(s) 119, 563, 1916
BseSI GKGCMC 4 cut(s) 472, 2456, 2489, 2564
BseXI GCAGC 7 cut(s) 518, 617, 1012, 1390, 1400, 2436, 2535
BseYI CCCAGC 2 cut(s) 85, 221
BsgI GTGCAG 1 cut(s) 1521
Bsh1236I CGCG 1 cut(s) 2626
Bsh1285I CGRYCG 2 cut(s) 1641, 1753
BshFI GGCC 4 cut(s) 839, 867, 1386, 1575
BsiEI CGRYCG 2 cut(s) 1641, 1753
BsiHKAI GWGCWC 1 cut(s) 745
BsiSI CCGG 2 cut(s) 1577, 1887
BslFI GGGAC 2 cut(s) 805, 1556
BslI CCNNNNNNNGG 6 cut(s) 295, 547, 1741, 2230, 2461, 2557
BsmAI GTCTC 6 cut(s) 443, 531, 1133, 1898, 2201, 2482
BsmFI GGGAC 2 cut(s) 805, 1556
BsmI GAATGC 3 cut(s) 851, 1441, 1511
BsnI GGCC 4 cut(s) 839, 867, 1386, 1575
Bso31I GGTCTC 1 cut(s) 443
Bsp1286I GDGCHC 5 cut(s) 472, 745, 2456, 2489, 2564
Bsp1407I TGTACA 1 cut(s) 1365
Bsp19I CCATGG 2 cut(s) 294, 1720
BspACI CCGC 8 cut(s) 77, 920, 1406, 1988, 2053, 2333, 2596, 2690
BspANI GGCC 4 cut(s) 839, 867, 1386, 1575
BspCNI CTCAG 3 cut(s) 947, 2109, 2242
BspFNI CGCG 1 cut(s) 2626
BspLI GGNNCC 2 cut(s) 1306, 2579
BspMI ACCTGC 1 cut(s) 541
BspOI GCTAGC 1 cut(s) 1443
BspPI GGATC 6 cut(s) 87, 157, 1033, 2572, 2585, 2624
BspTI CTTAAG 1 cut(s) 1946
BspTNI GGTCTC 1 cut(s) 443
BsrBI CCGCTC 1 cut(s) 2598
BsrDI GCAATG 1 cut(s) 846
BsrGI TGTACA 1 cut(s) 1365
BsrI ACTGG 9 cut(s) 443, 500, 1065, 1102, 1306, 1387, 2235, 2415, 2491
BssECI CCNNGG 4 cut(s) 294, 1720, 1753, 2211
BssNAI GTATAC 1 cut(s) 1787
BssNI GRCGYC 1 cut(s) 606
BssT1I CCWWGG 3 cut(s) 294, 1720, 2211
Bst1107I GTATAC 1 cut(s) 1787
Bst2UI CCWGG 1 cut(s) 1929
Bst4CI ACNGT 8 cut(s) 599, 1321, 1365, 1639, 1754, 1998, 2367, 2604
Bst6I CTCTTC 1 cut(s) 2246
BstACI GRCGYC 1 cut(s) 606
BstAFI CTTAAG 1 cut(s) 1946
BstAPI GCANNNNNTGC 2 cut(s) 455, 963
BstAUI TGTACA 1 cut(s) 1365
BstBAI YACGTR 1 cut(s) 1940
BstC8I GCNNGC 4 cut(s) 968, 1441, 1511, 2485
BstDEI CTNAG 7 cut(s) 175, 934, 1081, 1088, 1220, 2117, 2250
BstDSI CCRYGG 3 cut(s) 294, 1720, 1753
BstEII GGTNACC 1 cut(s) 1715
BstENI CCTNNNNNAGG 2 cut(s) 545, 1739
BstF5I GGATG 7 cut(s) 654, 793, 819, 922, 1379, 2135, 2170
BstFNI CGCG 1 cut(s) 2626
BstH2I RGCGCY 1 cut(s) 2654
BstHHI GCGC 3 cut(s) 71, 2628, 2653
BstMAI GTCTC 6 cut(s) 443, 531, 1133, 1898, 2201, 2482
BstMCI CGRYCG 2 cut(s) 1641, 1753
BstMWI GCNNNNNNNGC 6 cut(s) 455, 963, 1098, 2407, 2568, 2650
BstNI CCWGG 1 cut(s) 1929
BstNSI RCATGY 1 cut(s) 710
BstPI GGTNACC 1 cut(s) 1715
BstSCI CCNGG 3 cut(s) 1575, 1886, 1927
BstSFI CTRYAG 3 cut(s) 858, 1013, 2572
BstSLI GKGCMC 4 cut(s) 472, 2456, 2489, 2564
BstSNI TACGTA 1 cut(s) 1940
BstUI CGCG 1 cut(s) 2626
BstV1I GCAGC 7 cut(s) 518, 617, 1012, 1390, 1400, 2436, 2535
BstV2I GAAGAC 4 cut(s) 498, 672, 2286, 2384
BstX2I RGATCY 5 cut(s) 79, 162, 1038, 1084, 2577
BstXI CCANNNNNNTGG 1 cut(s) 982
BstYI RGATCY 5 cut(s) 79, 162, 1038, 1084, 2577
BstZ17I GTATAC 1 cut(s) 1787
BsuI GTATCC 1 cut(s) 46
BsuRI GGCC 4 cut(s) 839, 867, 1386, 1575
BtgI CCRYGG 3 cut(s) 294, 1720, 1753
BtrI CACGTC 1 cut(s) 2587
BtsCI GGATG 7 cut(s) 654, 793, 819, 922, 1379, 2135, 2170
BtsI GCAGTG 1 cut(s) 1109
BtsIMutI CAGTG 6 cut(s) 604, 1072, 1095, 1109, 1394, 1681
BveI ACCTGC 1 cut(s) 541
Cac8I GCNNGC 4 cut(s) 968, 1441, 1511, 2485
CaiI CAGNNNCTG 1 cut(s) 2230
CfoI GCGC 3 cut(s) 71, 2628, 2653
Cfr13I GGNCC 2 cut(s) 1574, 2168
CseI GACGC 1 cut(s) 614
Csp6I GTAC 5 cut(s) 1361, 1366, 1708, 2418, 2430
CviQI GTAC 5 cut(s) 1361, 1366, 1708, 2418, 2430
DdeI CTNAG 7 cut(s) 175, 934, 1081, 1088, 1220, 2117, 2250
DraI TTTAAA 1 cut(s) 1473
DraIII CACNNNGTG 1 cut(s) 2587
DrdI GACNNNNNNGTC 1 cut(s) 1840
DriI GACNNNNNGTC 1 cut(s) 610
DseDI GACNNNNNNGTC 1 cut(s) 1840
EaeI YGGCCR 3 cut(s) 837, 865, 1384
Eam1104I CTCTTC 1 cut(s) 2246
Eam1105I GACNNNNNGTC 1 cut(s) 610
EarI CTCTTC 1 cut(s) 2246
Eco105I TACGTA 1 cut(s) 1940
Eco130I CCWWGG 3 cut(s) 294, 1720, 2211
Eco31I GGTCTC 1 cut(s) 443
Eco32I GATATC 1 cut(s) 1338
Eco47I GGWCC 1 cut(s) 2168
Eco57I CTGAAG 1 cut(s) 983
Eco91I GGTNACC 1 cut(s) 1715
EcoNI CCTNNNNNAGG 2 cut(s) 545, 1739
EcoO65I GGTNACC 1 cut(s) 1715
EcoRI GAATTC 1 cut(s) 216
EcoRII CCWGG 1 cut(s) 1927
EcoRV GATATC 1 cut(s) 1338
EcoT14I CCWWGG 3 cut(s) 294, 1720, 2211
EcoT22I ATGCAT 1 cut(s) 819
ErhI CCWWGG 3 cut(s) 294, 1720, 2211
FalI AAGNNNNNCTT 6 cut(s) 57, 89, 361, 393, 602, 634
FaqI GGGAC 2 cut(s) 805, 1556
FauI CCCGC 2 cut(s) 1399, 2046
FauNDI CATATG 1 cut(s) 2159
FbaI TGATCA 1 cut(s) 2545
FblI GTMKAC 1 cut(s) 1786
Fnu4HI GCNGC 7 cut(s) 507, 631, 1026, 1404, 1414, 2425, 2524
FokI GGATG 7 cut(s) 641, 800, 826, 929, 1386, 2122, 2177
Fsp4HI GCNGC 7 cut(s) 507, 631, 1026, 1404, 1414, 2425, 2524
FspBI CTAG 6 cut(s) 390, 423, 756, 1440, 1836, 2540
GlaI GCGC 3 cut(s) 70, 2627, 2652
GluI GCNGC 7 cut(s) 507, 631, 1026, 1404, 1414, 2425, 2524
GsaI CCCAGC 2 cut(s) 89, 225
GsuI CTGGAG 1 cut(s) 1760
HaeII RGCGCY 1 cut(s) 2654
HaeIII GGCC 4 cut(s) 839, 867, 1386, 1575
HapII CCGG 2 cut(s) 1577, 1887
HgaI GACGC 1 cut(s) 614
HhaI GCGC 3 cut(s) 71, 2628, 2653
Hin1I GRCGYC 1 cut(s) 606
Hin6I GCGC 3 cut(s) 69, 2626, 2651
HinP1I GCGC 3 cut(s) 69, 2626, 2651
HincII GTYRAC 1 cut(s) 712
HindII GTYRAC 1 cut(s) 712
HindIII AAGCTT 4 cut(s) 768, 968, 1146, 1208
HpaII CCGG 2 cut(s) 1577, 1887
HphI GGTGA 7 cut(s) 256, 892, 1709, 1928, 1976, 1993, 2540
Hpy166II GTNNAC 4 cut(s) 119, 264, 712, 1787
Hpy188III TCNNGA 7 cut(s) 830, 1623, 1739, 2027, 2107, 2181, 2191
Hpy8I GTNNAC 4 cut(s) 119, 264, 712, 1787
Hpy99I CGWCG 2 cut(s) 608, 2588
HpyAV CCTTC 8 cut(s) 358, 366, 794, 920, 1477, 1745, 2075, 2641
HpyCH4III ACNGT 8 cut(s) 599, 1321, 1365, 1639, 1754, 1998, 2367, 2604
HpyCH4IV ACGT 6 cut(s) 1521, 1939, 2100, 2283, 2389, 2586
HpyF10VI GCNNNNNNNGC 6 cut(s) 455, 963, 1098, 2407, 2568, 2650
HpyF3I CTNAG 7 cut(s) 175, 934, 1081, 1088, 1220, 2117, 2250
HpySE526I ACGT 6 cut(s) 1521, 1939, 2100, 2283, 2389, 2586
Hsp92I GRCGYC 1 cut(s) 606
HspAI GCGC 3 cut(s) 69, 2626, 2651
Ksp22I TGATCA 1 cut(s) 2545
LmnI GCTCC 2 cut(s) 1903, 2179
Lsp1109I GCAGC 7 cut(s) 518, 617, 1012, 1390, 1400, 2436, 2535
LweI GCATC 4 cut(s) 331, 804, 907, 1220
MaeI CTAG 6 cut(s) 390, 423, 756, 1440, 1836, 2540
MaeII ACGT 6 cut(s) 1521, 1939, 2100, 2283, 2389, 2586
MaeIII GTNAC 5 cut(s) 599, 751, 1681, 1715, 2591
MbiI CCGCTC 1 cut(s) 2598
MfeI CAATTG 1 cut(s) 2273
MflI RGATCY 5 cut(s) 79, 162, 1038, 1084, 2577
MhlI GDGCHC 5 cut(s) 472, 745, 2456, 2489, 2564
MlsI TGGCCA 2 cut(s) 867, 1386
MluNI TGGCCA 2 cut(s) 867, 1386
MlyI GAGTC 7 cut(s) 116, 744, 1134, 1178, 1764, 2032, 2203
MmeI TCCRAC 3 cut(s) 1272, 1857, 2560
Mox20I TGGCCA 2 cut(s) 867, 1386
Mph1103I ATGCAT 1 cut(s) 819
MroXI GAANNNNTTC 2 cut(s) 2125, 2391
MscI TGGCCA 2 cut(s) 867, 1386
MslI CAYNNNNRTG 3 cut(s) 1158, 1752, 2472
Msp20I TGGCCA 2 cut(s) 867, 1386
MspCI CTTAAG 1 cut(s) 1946
MspI CCGG 2 cut(s) 1577, 1887
MspR9I CCNGG 3 cut(s) 1577, 1888, 1929
MssI GTTTAAAC 1 cut(s) 1473
MunI CAATTG 1 cut(s) 2273
Mva1269I GAATGC 3 cut(s) 851, 1441, 1511
MvaI CCWGG 1 cut(s) 1929
MvnI CGCG 1 cut(s) 2626
MwoI GCNNNNNNNGC 6 cut(s) 455, 963, 1098, 2407, 2568, 2650
NciI CCSGG 2 cut(s) 1577, 1888
NcoI CCATGG 2 cut(s) 294, 1720
NdeI CATATG 1 cut(s) 2159
NheI GCTAGC 1 cut(s) 1439
NlaIV GGNNCC 2 cut(s) 1306, 2579
NmuCI GTSAC 4 cut(s) 599, 751, 1681, 1715
NsiI ATGCAT 1 cut(s) 819
NspI RCATGY 1 cut(s) 710
OliI CACNNNNGTG 1 cut(s) 1752
PciI ACATGT 1 cut(s) 706
PctI GAATGC 3 cut(s) 851, 1441, 1511
PdmI GAANNNNTTC 2 cut(s) 2125, 2391
PfeI GAWTC 3 cut(s) 1216, 1605, 2184
PflMI CCANNNNNTGG 2 cut(s) 295, 2230
PkrI GCNGC 7 cut(s) 508, 632, 1027, 1405, 1415, 2426, 2525
PleI GAGTC 7 cut(s) 115, 743, 1134, 1177, 1764, 2031, 2202
PmeI GTTTAAAC 1 cut(s) 1473
PpsI GAGTC 7 cut(s) 115, 743, 1134, 1177, 1764, 2031, 2202
Ppu21I YACGTR 1 cut(s) 1940
PscI ACATGT 1 cut(s) 706
PshBI ATTAAT 1 cut(s) 1631
PsiI TTATAA 1 cut(s) 170
Psp1406I AACGTT 1 cut(s) 1521
Psp6I CCWGG 1 cut(s) 1927
PspEI GGTNACC 1 cut(s) 1715
PspFI CCCAGC 2 cut(s) 85, 221
PspGI CCWGG 1 cut(s) 1927
PspN4I GGNNCC 2 cut(s) 1306, 2579
PspPI GGNCC 2 cut(s) 1574, 2168
PsrI GAACNNNNNNTAC 2 cut(s) 1777, 1809
PstNI CAGNNNCTG 1 cut(s) 2230
PsuI RGATCY 5 cut(s) 79, 162, 1038, 1084, 2577
RsaI GTAC 5 cut(s) 1362, 1367, 1709, 2419, 2431
RsaNI GTAC 5 cut(s) 1361, 1366, 1708, 2418, 2430
RseI CAYNNNNRTG 3 cut(s) 1158, 1752, 2472
SatI GCNGC 7 cut(s) 507, 631, 1026, 1404, 1414, 2425, 2524
Sau96I GGNCC 2 cut(s) 1574, 2168
ScaI AGTACT 2 cut(s) 1362, 2431
SchI GAGTC 7 cut(s) 116, 744, 1134, 1178, 1764, 2032, 2203
ScrFI CCNGG 3 cut(s) 1577, 1888, 1929
SduI GDGCHC 5 cut(s) 472, 745, 2456, 2489, 2564
SfaNI GCATC 4 cut(s) 331, 804, 907, 1220
SfcI CTRYAG 3 cut(s) 858, 1013, 2572
SinI GGWCC 1 cut(s) 2168
SmiMI CAYNNNNRTG 3 cut(s) 1158, 1752, 2472
SmlI CTYRAG 5 cut(s) 518, 1211, 1329, 1772, 1946
SmoI CTYRAG 5 cut(s) 518, 1211, 1329, 1772, 1946
SnaBI TACGTA 1 cut(s) 1940
SsiI CCGC 8 cut(s) 77, 920, 1406, 1988, 2053, 2333, 2596, 2690
SspI AATATT 1 cut(s) 61
SspMI CTAG 6 cut(s) 390, 423, 756, 1440, 1836, 2540
StyD4I CCNGG 3 cut(s) 1575, 1886, 1927
StyI CCWWGG 3 cut(s) 294, 1720, 2211
TaaI ACNGT 8 cut(s) 599, 1321, 1365, 1639, 1754, 1998, 2367, 2604
TaiI ACGT 6 cut(s) 1524, 1942, 2103, 2286, 2392, 2589
TaqI TCGA 8 cut(s) 278, 511, 703, 829, 911, 1641, 2260, 2448
TaqII GACCGA 1 cut(s) 739
TatI WGTACW 4 cut(s) 1360, 1365, 2417, 2429
TfiI GAWTC 3 cut(s) 1216, 1605, 2184
TscAI CASTG 6 cut(s) 604, 1072, 1102, 1116, 1394, 1681
TseFI GTSAC 4 cut(s) 599, 751, 1681, 1715
TseI GCWGC 7 cut(s) 506, 630, 1025, 1403, 1413, 2424, 2523
Tsp45I GTSAC 4 cut(s) 599, 751, 1681, 1715
TspGWI ACGGA 2 cut(s) 155, 647
TspRI CASTG 6 cut(s) 604, 1072, 1102, 1116, 1394, 1681
Van91I CCANNNNNTGG 2 cut(s) 295, 2230
Vha464I CTTAAG 1 cut(s) 1946
VpaK11BI GGWCC 1 cut(s) 2168
VspI ATTAAT 1 cut(s) 1631
XagI CCTNNNNNAGG 2 cut(s) 545, 1739
XapI RAATTY 5 cut(s) 216, 312, 1250, 1270, 1279
XceI RCATGY 1 cut(s) 710
XmiI GTMKAC 1 cut(s) 1786
XmnI GAANNNNTTC 2 cut(s) 2125, 2391
XspI CTAG 6 cut(s) 390, 423, 756, 1440, 1836, 2540
ZrmI AGTACT 2 cut(s) 1362, 2431
Zsp2I ATGCAT 1 cut(s) 819
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.