RchiOBHm_Chr5g0078251

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000085
Physical Location & Seq
Reverse (-)
84174133 .. 84177509
3377 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35278

Sequence Viewer

Length: 3057 bp
ATGGCCTCCAGCTCCCAAATAGCCTGTGCTTCTCCTCCTTCATCAGATCCATGTTGGAAATATGATGTGTTTTTAAGTTTCAGGGGTGTAGACACTCGCAAGGGGATCACAGTCGACATACACGATCGGCTAAATAGGAGTGGAATCAAAACATTCATGGATGAGCAGGACCTTCAAGTAGGAGATGCTATTTCTCCCACACTCTTAGCAGCAATCAAAGAATCAAGGTTTGCAATTAACAGAATTTTGCCACTTTTCTATCACGTGGAGCCCTCTGATGTTCGATACCAGAACAAGGGGAGTTTCGAAGAAGCTTTCTCTAAGCATAAAACCTCTGGTCGACATGAATTAGAGAAGGTGAAGCAGTGGAGGGATGCTTTGTACAAAGTGGCAAATTTCTCTGGGTGGAATACATCTGATTATAAGACTCACAAAAAACTTGTTGACGCCATTGAGGAATTTCTCCGCAGTAAAGTTCTAACTGATGCAATTGAGTCCACAAGAGATTTCAAAACATTTAAAGCAACAAGAAAAGCCATGGATGAGGTTATGAAGGCGCTAAAAGATGACGAGGTCACTACCATTGGTGTCTACGGCATGGGAGGAGTTGGAAAGACAACCATGGTAAGACATGTCGGTGCACAAGCCCAAAAAAGGGGGATTTTTCATCATGTGATTATGGCAGTCGTATCCCAAAGCCCTGACTTGGAAAAAATTCAAAGAACATTGGCAGAACTGTTGGGAGTTAAATTGTCGGAGGTGACAGAAATTGGAAGAGCAGCTAGATTGAGTAAGCAAATGAGAAGAAATAAGATCCTTATAATCCTGGATGACATTTGGGAGAGAATGGACTTATCAAAAATAGGAGTACCTAGCTACAAGGAACTTCAAAAGTGCAATTCCAAAGTCCTACTTACCACAAGGATAAGGAATGTCTGTCATGTCATGAGATGCCAAAGAAATATCACCCTCAGTATCCTATCAAAAGGTGATTCTTGGACCTTGTTTATGAGAAATGCGGGAAGGTCTTTTGAATCCACCAATTTTGAGGATGTAGCGAGGAGGGTAGCTAGAGAATGCTGTGGTCTACCGATTGCATTGATAACAGTTGCAAGGGCACTCGGAGATAAAGACTTGGTGGAATGGAAAAGAGCAGTTCAACGACTAGAGAAGTCGCAAACTGCCAACCCTGACGATCAAGGAGATGCATCCAAATGTATAAAATTAAGCTATGATTACTTGAAAGATGAGGAGTACAAGTCATACTTCTTGCTCTGTTGCTTATTCCCAGAAGACTATGAAATCGAAATAGAAGACTTGTTCAGGTATGCGATAGGTAAAGGATTGTTTCGAGATGCCGACACACTTGAAGAAGCCAGAGAGAGAGCTGATTCCGTGGCCAGGCACCTTCAATATTCTTGCTTGCTTTTGGACGCAGACAGATCTTGGAGGACAGCAAGAATAAGATGTGTAAGGATGCATGATGTTGTCCGGGATACAGCCATTCAGATTGTGCAATCTGAACATGGGTTTTTGGTGAGAGCTGGCTGTGATTTAAAGGACTGGCCACGTCAATTATATGAAGGCTGTTCTGCAATCTCACTAATGCAGAATAAAATTAGCAAGCTACCCGAAAAATTGGTATGTCCAGAACTTCAGATTTTGTTACTAAACTCTAATTTCTCTATGGTAGAGATCCCAGAAACATTCTTCCAAGGTCCAAATAAGTTAACGGTTGTAGATCTTTCAAACACTGGCATCACTTTACTACCCGAATCATTCAGTCTCCTGATCAACCTCCAAGCTTTGTGTATAGATAGATGTCACAACCTTGACACTTCTATACTAGGAAAATTGAAAAAGCTTGAGATTCTTAGCATGAGATTCTGTCGTGTAAAGAAGTTGTCGAGAAAGATGGGAAATTTGATCAATCTAAGGATGTTGGATATCACTGATGCAGTTATTGATACAATTCCATCGAAGGTGATATCAAAGTTTCATAATTTAGAAGAACTGTACATGCAAGGATTTTGGAACTGGGGGAGTAAAGTTGAGGGAGAAGGAGAAGAAACCAATGCTGGTTTTGACGAGGTAACTAGCTTGTCAAATTTAAGAGTTTTGAAGGTTTGTATCTCCAAAGCAGAACGTATCCCTAAATATGTTGATTTCAAACCGAATTGGGTCGAGTTTGATATACTTATCGGTAGAGACGACTCGCAGAGAATCTTTAATCCATATGGTCAGAAGTCATTATCCTTGAAGCTTGTCACCATCAGTCGCTTACCGAACTGGTTTTGCAACGCGCTGATGAACACTGCAGAGAAGCTATGGTGGGAAGGCCGCCAAAATTTTGGTGACATATTTGTGGAATATGAGTTGATGAACACAATAATACGGGTTCCAAAAGAACCTGTGTTCGAGTACTTGGTAGAGTTGCATCTGTATTTCGTGAATGGCCTAGAGTTATGTGTTGGTGAATTGCCACTTGGGTCTCTATTTAATTTGAAGTTATTGGAGGTAAAATTTTGTTATAATTGGGGGAACGTATTTTTACCATCAAATTTGTTACTACCAAACCTAGAAGAACTAAATTGTCGTTTCACGGGCGGGATTGAATATGTGTTTGGGTATGAAGCGTTATATCAGCCAAAGCAATCAAAATTGAGAAAGATAGAGTTGAACTATCTGATGACAGTAAGAAGCATATGTCATGGACCTGCTCCAACTGCAATGTTTCAGGCTCTTCAAAGTTTGACCATTAGGCGGTGCAACTTCCAGGGAAGTCTCTTCACCTCTGATGTTGCTCAATGTCTTTCTCAATTGAATTATCTTGAACTACACGATTGCCCCTCTTTGGAAAGAATAGTTGAAGCAAGCAACAAGAAGATCATTCTTCCAAAATTGAGGCGATTGACTTTGTTGGTACTTCCAATGTTGTCCCATGACAGTGCTACTTTTGAGTGTCCTTCACTGGTATACTTGGACTTGTGGGGCTGCCCCAACTTTTCCGCCTCTTTTTCCGACTTCCACAGCAGGGAAACAAGGACCTACTATTAG

Protein Analysis

1018

Amino Acids

116.66

Weight (kDa)

8.33

Isoelectric Point (pI)

39.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 20 - 79 1.2e-13 TIR domain
TIR PF01582 82 - 173 3.1e-11 TIR domain
NB-ARC PF00931 178 - 341 1.7e-32 NB-ARC domain
LRR_8 PF13855 550 - 608 3.5e-06 Leucine rich repeat
LRR_14 PF23598 576 - 721 2.9e-12 Leucine-rich repeat region
LRR_RPS2 PF23247 821 - 949 1.9e-16 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 897 - 1006 1.8e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 423, 821, 2532
Acc36I ACCTGC 1 cut(s) 2725
AccB1I GGYRCC 1 cut(s) 1404
AccI GTMKAC 6 cut(s) 90, 114, 340, 591, 1087, 2976
AccII CGCG 1 cut(s) 2303
AciI CCGC 6 cut(s) 466, 1019, 2341, 2607, 2764, 3009
AclWI GGATC 4 cut(s) 41, 113, 808, 1690
AcoI YGGCCR 2 cut(s) 1398, 1565
AcsI RAATTY 9 cut(s) 243, 394, 458, 714, 1921, 2107, 2347, 2522, 2560
AcuI CTGAAG 1 cut(s) 1640
AcvI CACGTG 1 cut(s) 265
AcyI GRCGYC 1 cut(s) 447
AfaI GTAC 6 cut(s) 383, 870, 1256, 2018, 2423, 2925
AfiI CCNNNNNNNGG 8 cut(s) 295, 654, 655, 706, 1401, 2763, 2854, 3034
AflIII ACRYGT 1 cut(s) 631
AjiI CACGTC 1 cut(s) 1571
AjnI CCWGG 3 cut(s) 825, 1400, 2775
AjuI GAANNNNNNNTTGG 4 cut(s) 2469, 2501, 2607, 2639
Alw21I GWGCWC 1 cut(s) 643
Alw26I GTCTC 4 cut(s) 1790, 2202, 2496, 2789
Alw44I GTGCAC 1 cut(s) 639
AlwI GGATC 4 cut(s) 41, 113, 808, 1690
AoxI GGCC 5 cut(s) 3, 1398, 1565, 2338, 2455
ApaLI GTGCAC 1 cut(s) 639
ApeKI GCWGC 3 cut(s) 209, 779, 2994
ApoI RAATTY 9 cut(s) 243, 394, 458, 714, 1921, 2107, 2347, 2522, 2560
Asp700I GAANNNNTTC 1 cut(s) 714
AspLEI GCGC 2 cut(s) 559, 2305
AspS9I GGNCC 5 cut(s) 169, 999, 1718, 2714, 3045
AsuC2I CCSGG 1 cut(s) 1493
AsuII TTCGAA 1 cut(s) 306
AvaII GGWCC 5 cut(s) 169, 999, 1718, 2714, 3045
BaeGI GKGCMC 2 cut(s) 643, 1120
BalI TGGCCA 2 cut(s) 1400, 1567
BanI GGYRCC 1 cut(s) 1404
BanII GRGCYC 1 cut(s) 273
BbrPI CACGTG 1 cut(s) 265
BbsI GAAGAC 2 cut(s) 1299, 1320
Bbv12I GWGCWC 1 cut(s) 643
BbvI GCAGC 3 cut(s) 221, 791, 2981
BccI CCATC 4 cut(s) 1909, 1984, 2279, 2563
BceAI ACGGC 1 cut(s) 610
BciT130I CCWGG 3 cut(s) 827, 1402, 2777
BciVI GTATCC 4 cut(s) 700, 986, 1489, 2159
BclI TGATCA 2 cut(s) 1791, 1926
BcnI CCSGG 1 cut(s) 1493
BcoDI GTCTC 4 cut(s) 1790, 2202, 2496, 2789
BfaI CTAG 8 cut(s) 783, 873, 1071, 1166, 1847, 2097, 2459, 2579
BfmI CTRYAG 1 cut(s) 2316
BfoI RGCGCY 1 cut(s) 560
BfuAI ACCTGC 1 cut(s) 2725
BfuI GTATCC 4 cut(s) 700, 986, 1489, 2159
BglII AGATCT 2 cut(s) 1442, 1741
BisI GCNGC 4 cut(s) 210, 780, 2341, 2995
BlsI GCNGC 4 cut(s) 211, 781, 2342, 2996
BmcAI AGTACT 1 cut(s) 2423
Bme1390I CCNGG 4 cut(s) 827, 1402, 1493, 2777
Bme18I GGWCC 5 cut(s) 169, 999, 1718, 2714, 3045
BmgBI CACGTC 1 cut(s) 1571
BmgT120I GGNCC 5 cut(s) 169, 999, 1718, 2714, 3045
BmiI GGNNCC 3 cut(s) 270, 1406, 2400
BmrFI CCNGG 4 cut(s) 827, 1402, 1493, 2777
BmrI ACTGGG 1 cut(s) 2047
BmuI ACTGGG 1 cut(s) 2047
BpiI GAAGAC 2 cut(s) 1299, 1320
Bpu14I TTCGAA 1 cut(s) 306
BpuEI CTTGAG 1 cut(s) 1886
BpuMI CCSGG 1 cut(s) 1493
BsaAI YACGTR 1 cut(s) 265
BsaHI GRCGYC 1 cut(s) 447
BsaI GGTCTC 1 cut(s) 2496
BsaJI CCNNGG 5 cut(s) 537, 621, 1395, 1714, 2776
BsaXI ACNNNNNCTCC 2 cut(s) 1244, 1274
Bsc4I CCNNNNNNNGG 8 cut(s) 295, 654, 655, 706, 1401, 2763, 2854, 3034
Bse1I ACTGG 5 cut(s) 1568, 1759, 2042, 2294, 2976
Bse3DI GCAATG 1 cut(s) 2736
BseBI CCWGG 3 cut(s) 827, 1402, 2777
BseDI CCNNGG 5 cut(s) 537, 621, 1395, 1714, 2776
BseGI GGATG 8 cut(s) 166, 379, 547, 835, 1057, 1208, 1482, 1944
BseLI CCNNNNNNNGG 8 cut(s) 295, 654, 655, 706, 1401, 2763, 2854, 3034
BseMI GCAATG 1 cut(s) 2736
BseMII CTCAG 1 cut(s) 985
BseNI ACTGG 5 cut(s) 1568, 1759, 2042, 2294, 2976
BseRI GAGGAG 4 cut(s) 24, 618, 1075, 1265
BseSI GKGCMC 2 cut(s) 643, 1120
BseXI GCAGC 3 cut(s) 221, 791, 2981
Bsh1236I CGCG 1 cut(s) 2303
Bsh1285I CGRYCG 1 cut(s) 127
BshFI GGCC 5 cut(s) 5, 1400, 1567, 2340, 2457
BshNI GGYRCC 1 cut(s) 1404
BsiEI CGRYCG 1 cut(s) 127
BsiHKAI GWGCWC 1 cut(s) 643
BsiSI CCGG 1 cut(s) 1492
BslFI GGGAC 1 cut(s) 2923
BslI CCNNNNNNNGG 8 cut(s) 295, 654, 655, 706, 1401, 2763, 2854, 3034
BsmAI GTCTC 4 cut(s) 1790, 2202, 2496, 2789
BsmBI CGTCTC 1 cut(s) 2202
BsmFI GGGAC 1 cut(s) 2923
BsmI GAATGC 1 cut(s) 1082
BsnI GGCC 5 cut(s) 5, 1400, 1567, 2340, 2457
Bso31I GGTCTC 1 cut(s) 2496
Bsp119I TTCGAA 1 cut(s) 306
Bsp1286I GDGCHC 3 cut(s) 273, 643, 1120
Bsp1407I TGTACA 2 cut(s) 381, 2016
Bsp19I CCATGG 2 cut(s) 537, 621
BspACI CCGC 6 cut(s) 466, 1019, 2341, 2607, 2764, 3009
BspANI GGCC 5 cut(s) 5, 1400, 1567, 2340, 2457
BspCNI CTCAG 1 cut(s) 984
BspFNI CGCG 1 cut(s) 2303
BspHI TCATGA 1 cut(s) 945
BspLI GGNNCC 3 cut(s) 270, 1406, 2400
BspMAI CTGCAG 1 cut(s) 2320
BspMI ACCTGC 1 cut(s) 2725
BspPI GGATC 4 cut(s) 41, 113, 808, 1690
BspQI GCTCTTC 2 cut(s) 769, 2748
BspT104I TTCGAA 1 cut(s) 306
BspT107I GGYRCC 1 cut(s) 1404
BspTNI GGTCTC 1 cut(s) 2496
BsrDI GCAATG 1 cut(s) 2736
BsrGI TGTACA 2 cut(s) 381, 2016
BsrI ACTGG 5 cut(s) 1568, 1759, 2042, 2294, 2976
BssECI CCNNGG 5 cut(s) 537, 621, 1395, 1714, 2776
BssNAI GTATAC 1 cut(s) 2977
BssNI GRCGYC 1 cut(s) 447
BssT1I CCWWGG 3 cut(s) 537, 621, 1714
Bst1107I GTATAC 1 cut(s) 2977
Bst2UI CCWGG 3 cut(s) 827, 1402, 2777
Bst4CI ACNGT 7 cut(s) 112, 738, 1108, 1735, 2016, 2695, 2948
Bst6I CTCTTC 3 cut(s) 769, 2748, 2792
BstACI GRCGYC 1 cut(s) 447
BstAUI TGTACA 2 cut(s) 381, 2016
BstBAI YACGTR 1 cut(s) 265
BstBI TTCGAA 1 cut(s) 306
BstC8I GCNNGC 4 cut(s) 1424, 1546, 1625, 2875
BstDEI CTNAG 5 cut(s) 205, 321, 971, 1874, 1934
BstDSI CCRYGG 3 cut(s) 537, 621, 1395
BstF5I GGATG 8 cut(s) 166, 379, 547, 835, 1057, 1208, 1482, 1944
BstFNI CGCG 1 cut(s) 2303
BstH2I RGCGCY 1 cut(s) 560
BstHHI GCGC 2 cut(s) 559, 2305
BstMAI GTCTC 4 cut(s) 1790, 2202, 2496, 2789
BstMCI CGRYCG 1 cut(s) 127
BstMWI GCNNNNNNNGC 1 cut(s) 2726
BstNI CCWGG 3 cut(s) 827, 1402, 2777
BstNSI RCATGY 2 cut(s) 635, 2023
BstSCI CCNGG 4 cut(s) 825, 1400, 1491, 2775
BstSFI CTRYAG 1 cut(s) 2316
BstSLI GKGCMC 2 cut(s) 643, 1120
BstUI CGCG 1 cut(s) 2303
BstV1I GCAGC 3 cut(s) 221, 791, 2981
BstV2I GAAGAC 2 cut(s) 1299, 1320
BstX2I RGATCY 5 cut(s) 46, 813, 1442, 1695, 1741
BstXI CCANNNNNNTGG 1 cut(s) 2351
BstYI RGATCY 5 cut(s) 46, 813, 1442, 1695, 1741
BstZ17I GTATAC 1 cut(s) 2977
BsuI GTATCC 4 cut(s) 700, 986, 1489, 2159
BsuRI GGCC 5 cut(s) 5, 1400, 1567, 2340, 2457
BtgI CCRYGG 3 cut(s) 537, 621, 1395
BtrI CACGTC 1 cut(s) 1571
BtsCI GGATG 8 cut(s) 166, 379, 547, 835, 1057, 1208, 1482, 1944
BtsI GCAGTG 2 cut(s) 371, 2313
BtsIMutI CAGTG 6 cut(s) 371, 1752, 1950, 2313, 2953, 2969
BveI ACCTGC 1 cut(s) 2725
Cac8I GCNNGC 4 cut(s) 1424, 1546, 1625, 2875
CciI TCATGA 1 cut(s) 945
CfoI GCGC 2 cut(s) 559, 2305
Cfr13I GGNCC 5 cut(s) 169, 999, 1718, 2714, 3045
CseI GACGC 2 cut(s) 455, 1442
Csp6I GTAC 6 cut(s) 382, 869, 1255, 2017, 2422, 2924
CspCI CAANNNNNGTGG 2 cut(s) 1027, 1062
CviQI GTAC 6 cut(s) 382, 869, 1255, 2017, 2422, 2924
DdeI CTNAG 5 cut(s) 205, 321, 971, 1874, 1934
DraI TTTAAA 2 cut(s) 520, 1557
EaeI YGGCCR 2 cut(s) 1398, 1565
Eam1104I CTCTTC 3 cut(s) 769, 2748, 2792
EarI CTCTTC 3 cut(s) 769, 2748, 2792
EciI GGCGGA 1 cut(s) 2998
Eco130I CCWWGG 3 cut(s) 537, 621, 1714
Eco24I GRGCYC 1 cut(s) 273
Eco31I GGTCTC 1 cut(s) 2496
Eco32I GATATC 2 cut(s) 1948, 1989
Eco47I GGWCC 5 cut(s) 169, 999, 1718, 2714, 3045
Eco57I CTGAAG 1 cut(s) 1640
Eco72I CACGTG 1 cut(s) 265
EcoO109I RGGNCCY 2 cut(s) 169, 3045
EcoRII CCWGG 3 cut(s) 825, 1400, 2775
EcoRV GATATC 2 cut(s) 1948, 1989
EcoT14I CCWWGG 3 cut(s) 537, 621, 1714
EcoT22I ATGCAT 2 cut(s) 1210, 1482
EcoT38I GRGCYC 1 cut(s) 273
ErhI CCWWGG 3 cut(s) 537, 621, 1714
Esp3I CGTCTC 1 cut(s) 2202
FalI AAGNNNNNCTT 4 cut(s) 897, 929, 1250, 1282
FaqI GGGAC 1 cut(s) 2923
FauI CCCGC 2 cut(s) 1012, 2600
FauNDI CATATG 2 cut(s) 2236, 2705
FbaI TGATCA 2 cut(s) 1791, 1926
FblI GTMKAC 6 cut(s) 90, 114, 340, 591, 1087, 2976
Fnu4HI GCNGC 4 cut(s) 210, 780, 2341, 2995
FokI GGATG 8 cut(s) 173, 386, 554, 842, 1064, 1195, 1489, 1951
FriOI GRGCYC 1 cut(s) 273
Fsp4HI GCNGC 4 cut(s) 210, 780, 2341, 2995
FspBI CTAG 8 cut(s) 783, 873, 1071, 1166, 1847, 2097, 2459, 2579
GlaI GCGC 2 cut(s) 558, 2304
GluI GCNGC 4 cut(s) 210, 780, 2341, 2995
HaeII RGCGCY 1 cut(s) 560
HaeIII GGCC 5 cut(s) 5, 1400, 1567, 2340, 2457
HapII CCGG 1 cut(s) 1492
HgaI GACGC 2 cut(s) 455, 1442
HhaI GCGC 2 cut(s) 559, 2305
Hin1I GRCGYC 1 cut(s) 447
Hin6I GCGC 2 cut(s) 557, 2303
HinP1I GCGC 2 cut(s) 557, 2303
HincII GTYRAC 4 cut(s) 115, 341, 445, 1731
HindII GTYRAC 4 cut(s) 115, 341, 445, 1731
HindIII AAGCTT 4 cut(s) 312, 1803, 1862, 2261
HpaI GTTAAC 1 cut(s) 1731
HpaII CCGG 1 cut(s) 1492
Hpy188III TCNNGA 7 cut(s) 946, 1352, 1649, 1789, 1908, 2449, 2831
HpyCH4III ACNGT 7 cut(s) 112, 738, 1108, 1735, 2016, 2695, 2948
HpyCH4IV ACGT 4 cut(s) 264, 1570, 2146, 2544
HpyF10VI GCNNNNNNNGC 1 cut(s) 2726
HpyF3I CTNAG 5 cut(s) 205, 321, 971, 1874, 1934
HpySE526I ACGT 4 cut(s) 264, 1570, 2146, 2544
Hsp92I GRCGYC 1 cut(s) 447
HspAI GCGC 2 cut(s) 557, 2303
Ksp22I TGATCA 2 cut(s) 1791, 1926
KspAI GTTAAC 1 cut(s) 1731
LguI GCTCTTC 2 cut(s) 769, 2748
LmnI GCTCC 3 cut(s) 17, 268, 2725
Lsp1109I GCAGC 3 cut(s) 221, 791, 2981
MaeI CTAG 8 cut(s) 783, 873, 1071, 1166, 1847, 2097, 2459, 2579
MaeII ACGT 4 cut(s) 264, 1570, 2146, 2544
MaeIII GTNAC 8 cut(s) 574, 760, 1665, 1823, 2092, 2266, 2354, 2565
MfeI CAATTG 2 cut(s) 489, 2819
MflI RGATCY 5 cut(s) 46, 813, 1442, 1695, 1741
MhlI GDGCHC 3 cut(s) 273, 643, 1120
MlsI TGGCCA 2 cut(s) 1400, 1567
MluNI TGGCCA 2 cut(s) 1400, 1567
MlyI GAGTC 3 cut(s) 421, 503, 2207
MmeI TCCRAC 6 cut(s) 35, 589, 735, 1923, 2747, 3045
Mox20I TGGCCA 2 cut(s) 1400, 1567
Mph1103I ATGCAT 2 cut(s) 1210, 1482
MroXI GAANNNNTTC 1 cut(s) 714
MscI TGGCCA 2 cut(s) 1400, 1567
MslI CAYNNNNRTG 4 cut(s) 636, 1213, 2363, 2946
Msp20I TGGCCA 2 cut(s) 1400, 1567
MspI CCGG 1 cut(s) 1492
MspR9I CCNGG 4 cut(s) 827, 1402, 1493, 2777
MunI CAATTG 2 cut(s) 489, 2819
Mva1269I GAATGC 1 cut(s) 1082
MvaI CCWGG 3 cut(s) 827, 1402, 2777
MvnI CGCG 1 cut(s) 2303
MwoI GCNNNNNNNGC 1 cut(s) 2726
NciI CCSGG 1 cut(s) 1493
NcoI CCATGG 2 cut(s) 537, 621
NdeI CATATG 2 cut(s) 2236, 2705
NlaIV GGNNCC 3 cut(s) 270, 1406, 2400
NmuCI GTSAC 5 cut(s) 574, 760, 1823, 2266, 2354
NsiI ATGCAT 2 cut(s) 1210, 1482
NspI RCATGY 2 cut(s) 635, 2023
NspV TTCGAA 1 cut(s) 306
PagI TCATGA 1 cut(s) 945
PciI ACATGT 1 cut(s) 631
PciSI GCTCTTC 2 cut(s) 769, 2748
PcsI WCGNNNNNNNCGW 2 cut(s) 120, 2208
PctI GAATGC 1 cut(s) 1082
PdmI GAANNNNTTC 1 cut(s) 714
PfeI GAWTC 9 cut(s) 144, 221, 992, 1034, 1391, 1775, 1870, 1884, 2223
PflFI GACNNNGTC 1 cut(s) 572
PfoI TCCNGGA 2 cut(s) 825, 1491
PkrI GCNGC 4 cut(s) 211, 781, 2342, 2996
Ple19I CGATCG 1 cut(s) 127
PleI GAGTC 3 cut(s) 421, 502, 2207
PmaCI CACGTG 1 cut(s) 265
PmlI CACGTG 1 cut(s) 265
PpsI GAGTC 3 cut(s) 421, 502, 2207
Ppu21I YACGTR 1 cut(s) 265
PpuMI RGGWCCY 2 cut(s) 169, 3045
PscI ACATGT 1 cut(s) 631
PsiI TTATAA 3 cut(s) 423, 821, 2532
Psp5II RGGWCCY 2 cut(s) 169, 3045
Psp6I CCWGG 3 cut(s) 825, 1400, 2775
PspCI CACGTG 1 cut(s) 265
PspGI CCWGG 3 cut(s) 825, 1400, 2775
PspN4I GGNNCC 3 cut(s) 270, 1406, 2400
PspPI GGNCC 5 cut(s) 169, 999, 1718, 2714, 3045
PspPPI RGGWCCY 2 cut(s) 169, 3045
PstI CTGCAG 1 cut(s) 2320
PsuI RGATCY 5 cut(s) 46, 813, 1442, 1695, 1741
PsyI GACNNNGTC 1 cut(s) 572
PvuI CGATCG 1 cut(s) 127
RsaI GTAC 6 cut(s) 383, 870, 1256, 2018, 2423, 2925
RsaNI GTAC 6 cut(s) 382, 869, 1255, 2017, 2422, 2924
RseI CAYNNNNRTG 4 cut(s) 636, 1213, 2363, 2946
SalI GTCGAC 2 cut(s) 113, 339
SapI GCTCTTC 2 cut(s) 769, 2748
SatI GCNGC 4 cut(s) 210, 780, 2341, 2995
Sau96I GGNCC 5 cut(s) 169, 999, 1718, 2714, 3045
ScaI AGTACT 1 cut(s) 2423
SchI GAGTC 3 cut(s) 421, 503, 2207
ScrFI CCNGG 4 cut(s) 827, 1402, 1493, 2777
SduI GDGCHC 3 cut(s) 273, 643, 1120
SfcI CTRYAG 1 cut(s) 2316
SfuI TTCGAA 1 cut(s) 306
SinI GGWCC 5 cut(s) 169, 999, 1718, 2714, 3045
SmiMI CAYNNNNRTG 4 cut(s) 636, 1213, 2363, 2946
SmlI CTYRAG 1 cut(s) 1865
SmoI CTYRAG 1 cut(s) 1865
SsiI CCGC 6 cut(s) 466, 1019, 2341, 2607, 2764, 3009
SspI AATATT 1 cut(s) 1415
SspMI CTAG 8 cut(s) 783, 873, 1071, 1166, 1847, 2097, 2459, 2579
StyD4I CCNGG 4 cut(s) 825, 1400, 1491, 2775
StyI CCWWGG 3 cut(s) 537, 621, 1714
TaaI ACNGT 7 cut(s) 112, 738, 1108, 1735, 2016, 2695, 2948
TaiI ACGT 4 cut(s) 267, 1573, 2149, 2547
TatI WGTACW 4 cut(s) 381, 1254, 2016, 2421
TauI GCSGC 1 cut(s) 2343
TfiI GAWTC 9 cut(s) 144, 221, 992, 1034, 1391, 1775, 1870, 1884, 2223
TscAI CASTG 6 cut(s) 371, 1759, 1957, 2320, 2953, 2976
TseFI GTSAC 5 cut(s) 574, 760, 1823, 2266, 2354
TseI GCWGC 3 cut(s) 209, 779, 2994
Tsp45I GTSAC 5 cut(s) 574, 760, 1823, 2266, 2354
TspGWI ACGGA 1 cut(s) 1384
TspRI CASTG 6 cut(s) 371, 1759, 1957, 2320, 2953, 2976
Tth111I GACNNNGTC 1 cut(s) 572
VneI GTGCAC 1 cut(s) 639
VpaK11BI GGWCC 5 cut(s) 169, 999, 1718, 2714, 3045
XapI RAATTY 9 cut(s) 243, 394, 458, 714, 1921, 2107, 2347, 2522, 2560
XceI RCATGY 2 cut(s) 635, 2023
XmiI GTMKAC 6 cut(s) 90, 114, 340, 591, 1087, 2976
XmnI GAANNNNTTC 1 cut(s) 714
XspI CTAG 8 cut(s) 783, 873, 1071, 1166, 1847, 2097, 2459, 2579
ZrmI AGTACT 1 cut(s) 2423
Zsp2I ATGCAT 2 cut(s) 1210, 1482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.