Rmu_ssc0000170.1_g000029

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000170.1
OG0000085
Physical Location & Seq
Reverse (-)
129841 .. 134056
4216 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000170.1_g000029.1.cds

Sequence Viewer

Length: 3327 bp
atggctttgagcagtccaagagcctctgcatctattccttcatcgcctgctgatcctcagttgaagcatgatgtgtttctcagtttcaggggagaggatactcggaaggggattacatcccaattatatgaggaactgcaacacaggagaggaataaaaacattcaaggatgataaagagctcgaagtaggggtggctatttctcctagtctcctaatggcaatcgaagaatccaggtttgctatcgttattctgtcgccaaactatgcttcttctccttggtgtctggatgaacttacaaggatttgtctatgcatggaaggcaaggacacaattctgccactattttatcatgtggatccatctgatgtacgacaccaaaagaacagttttgaagtggctttcactaagcatgaggaaaattttaggcaaccgaaggacaaggtgaagcagtggagagttgctttacagaaagtggccaatgtatccgggtggaatttaaagaattacgggtctgatggagaacttgtcaaagatattgtggaatttgtgtgcagtaaagtacaaccgatagaatttgagtctacactgtacacagcaggttttgaaccatttgaagcaacaatacaagccatggaagaggtcacgaaggcactgaaaaatgatgacatcactgccattggggttgagggcatgggaggggttggcaagacaaccatggtgaaatatgttgctgcacaagcactgaaaaatgggcattttaatcatgtgattatggctgtcatatcccaaagccccgacttgttaagaattcaaggcacattcgcagaccttctgcacttgagattgcatgaggagacattgattggaagagctggtaaattgagagctaggatgatgagagcaaacaagatcctcataatcttagatgacatgtgggacgaaattgatttgtcaagcataggagttccaaactacagcgagcttcaaagatgccattccaaagtcatgttgaccacaagaagaagaaatgtatgtcacgtcatgagaattcaagcacaaattactctagatatcctttccgaagaagattcttggactttgtttgtaaaaaatgcagcatcatcttttcaggaatcaaccattttctatgatatagcgagaaaggtagctagggaatgtgctggtctaccaattgcattgatagcagtttcaaaagcccttggagataaagacatagaagaatggaaagaagcagctcaacgcctagagaggtcccaaacagccaatcttgatgacaagggagatgtattcaaatgcataaagttcagctatgattacttgagatctaatgatgccaagtcatgtttcttgctttgctgcctattcccagaagatgaagatatccaaataacagacttgatgaaatatgcgctcgggaaaggattgtttacagatgccaacacactagaagaagctcgaggtagagcacattcagtggtcaagtaccttaaagcttcttgcttgcttttggatagtactgaggatgggtgcgtaaggatgcatgatgtcatccgggatacggccatgtcaattgcattgtctgaagatggccatggttttttggtaaaagctggctgtaaactaaagaattggccaaggggtatgcatgaaggctactctgcaatctcactaatgggaaacaagattcgcaagcttcccgagaatttggtatgttcaaaactccagattttattactacagactaattatgatatacaagagattccagaaagctttttccaaagtcctagttcattgatggtcttggattttagcaaaactagtatttctttactaccccaatcattgagtctcctaaccaacctccaagctttatatttggattcttgcaagaaaataattgacatatcaatacttggaaacctgaagaagcttgagattcttagtatgagaaaatttcctcttatggaattgccagaagaaatagggcatttggctaatcttaggatgttggacgtcactggtggatgtgttgatacgattccatctgaggtgttatcaaagttgtatagattagaagaactatacatgcaatgtgattttgggcattggtggagtgaagttgagggaccagaagaaaataatatttgctttgttcagttaaccagcttggaatatttaattatcttgaaggttaacatatcttctgcagatttcttgcctaaagacattgaactttatccaaattgggtaaactttaatataggtatctgcagaaaatcattggccatacaataccattgggttaatttgtttttagatgatgattcaagaaccttgactcttgacacaacaatcactacgttaccagattggtttatcaaggtcgtaacagcgaaaacagagaaactacagtacgtagagtgcaggggcttatgtaacattcttttggaacatgaacatggcagattagattgtctgaagtatctcactctatttgggccccataggaggttgaaaaagttgatgaatacaacaacattagttcttccaaataaatcagtgttcaagaatttggaagtcttggatattcaagtggactacctgaaggaattatgtgttggtgaattaccaacggggtctctacgcaatcttaaggtactgaaagtggagaaatgttatagtatggtgaatgcagttgtctcatcaaatttgttgaagagactacaaaatctggaaacactaaactgcggtttcattattcaactggcatatgtatttgatactgaagggctcgagccagagcaacttgtgctgacaaatttgagagaattgatattgcgtgatctctggaaattaagaaaaatatggcaaggtccggctcctcattcagtcttctgtaatcttaatattttggcatttcgttactgccataagctgaaaagtcttttgccgcaccatgtagctcaatgtcttttgcaattaaaacacctttgggtagaggagtgctgtggcctggacagagttattgaaccaagtgagcaaactgagagtcaaatggttgtatttccaagattaaaggacatggttttgagaaggcttccacaactcacaagattctacagaagtgctactatcgatcatgttgagtgcccttcgttggagcacttgtatgtggacaaatgcccccagttctccacccctgctgcttctgacttccacagcaggaaccaagtccaagtcaacgataaagaacagtttgtatctctaatgagaagaatgatggactcgtatggcttctga

Protein Analysis

1108

Amino Acids

126.29

Weight (kDa)

6.18

Isoelectric Point (pI)

47.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2043
Acc36I ACCTGC 1 cut(s) 590
AccI GTMKAC 2 cut(s) 584, 1189
AciI CCGC 2 cut(s) 2775, 2978
AclWI GGATC 4 cut(s) 47, 353, 366, 907
AcoI YGGCCR 5 cut(s) 477, 1584, 1612, 1655, 2310
AcuI CTGAAG 5 cut(s) 1626, 1970, 2525, 2651, 2832
AcyI GRCGYC 1 cut(s) 2040
AfaI GTAC 7 cut(s) 372, 564, 593, 1508, 1540, 2441, 2685
AfiI CCNNNNNNNGG 3 cut(s) 1582, 2535, 3184
AflII CTTAAG 1 cut(s) 2678
AflIII ACRYGT 1 cut(s) 933
AhlI ACTAGT 1 cut(s) 1844
AjiI CACGTC 1 cut(s) 1042
AjnI CCWGG 2 cut(s) 233, 3039
AjuI GAANNNNNNNTTGG 6 cut(s) 253, 285, 1352, 1384, 2628, 2660
Alw21I GWGCWC 3 cut(s) 183, 1492, 3192
Alw26I GTCTC 6 cut(s) 215, 851, 1880, 2670, 2731, 2740
AlwI GGATC 4 cut(s) 47, 353, 366, 907
Ama87I CYCGRG 4 cut(s) 1436, 1479, 1721, 2819
AoxI GGCC 7 cut(s) 477, 1584, 1612, 1655, 2310, 2525, 3037
ApaI GGGCCC 1 cut(s) 2529
ApeKI GCWGC 5 cut(s) 734, 1118, 1256, 1380, 3230
ArsI GACNNNNNNTTYG 2 cut(s) 3061, 3093
AspLEI GCGC 1 cut(s) 1435
AspS9I GGNCC 5 cut(s) 1275, 2153, 2525, 2526, 2900
AsuC2I CCSGG 2 cut(s) 490, 1577
AsuHPI GGTGA 4 cut(s) 457, 733, 2660, 2725
AvaI CYCGRG 4 cut(s) 1436, 1479, 1721, 2819
AvaII GGWCC 3 cut(s) 1275, 2153, 2900
BaeGI GKGCMC 2 cut(s) 2529, 3179
BalI TGGCCA 4 cut(s) 479, 1614, 1657, 2312
BamHI GGATCC 1 cut(s) 358
BanII GRGCYC 3 cut(s) 183, 2529, 2820
BarI GAAGNNNNNNTAC 2 cut(s) 609, 641
BbsI GAAGAC 1 cut(s) 2911
Bbv12I GWGCWC 3 cut(s) 183, 1492, 3192
BbvI GCAGC 5 cut(s) 721, 1130, 1268, 1367, 3217
BccI CCATC 7 cut(s) 370, 512, 1541, 1604, 1816, 2077, 3301
BceAI ACGGC 1 cut(s) 1599
BciT130I CCWGG 2 cut(s) 235, 3041
BciVI GTATCC 3 cut(s) 91, 496, 1573
BcnI CCSGG 2 cut(s) 490, 1577
BcoDI GTCTC 6 cut(s) 215, 851, 1880, 2670, 2731, 2740
BcuI ACTAGT 1 cut(s) 1844
BfaI CTAG 8 cut(s) 207, 891, 1070, 1173, 1268, 1469, 1812, 1845
BfmI CTRYAG 6 cut(s) 976, 1760, 2232, 2296, 2435, 3145
BfrI CTTAAG 1 cut(s) 2678
BfuAI ACCTGC 1 cut(s) 590
BfuI GTATCC 3 cut(s) 91, 496, 1573
BglII AGATCT 1 cut(s) 1346
BisI GCNGC 6 cut(s) 735, 1119, 1257, 1381, 2978, 3231
BlsI GCNGC 6 cut(s) 736, 1120, 1258, 1382, 2979, 3232
BmcAI AGTACT 1 cut(s) 1540
Bme1390I CCNGG 4 cut(s) 235, 490, 1577, 3041
Bme18I GGWCC 3 cut(s) 1275, 2153, 2900
BmeT110I CYCGRG 4 cut(s) 1436, 1479, 1721, 2819
BmgBI CACGTC 1 cut(s) 1042
BmgT120I GGNCC 5 cut(s) 1275, 2153, 2525, 2526, 2900
BmiI GGNNCC 7 cut(s) 360, 1277, 2154, 2527, 2528, 2907, 3254
BmrFI CCNGG 4 cut(s) 235, 490, 1577, 3041
BmrI ACTGGG 1 cut(s) 3208
BmsI GCATC 6 cut(s) 38, 983, 1130, 1345, 1447, 1551
BmuI ACTGGG 1 cut(s) 3208
BpiI GAAGAC 1 cut(s) 2911
BpmI CTGGAG 1 cut(s) 1730
BpuEI CTTGAG 3 cut(s) 862, 1363, 1979
BpuMI CCSGG 2 cut(s) 490, 1577
Bsa29I ATCGAT 1 cut(s) 3162
BsaAI YACGTR 1 cut(s) 2443
BsaHI GRCGYC 1 cut(s) 2040
BsaI GGTCTC 1 cut(s) 2670
BsaJI CCNNGG 6 cut(s) 278, 633, 717, 1222, 1615, 1658
BsaXI ACNNNNNCTCC 4 cut(s) 513, 543, 3179, 3209
Bsc4I CCNNNNNNNGG 3 cut(s) 1582, 2535, 3184
Bse1I ACTGG 3 cut(s) 2050, 2796, 3214
Bse3DI GCAATG 1 cut(s) 2123
BseBI CCWGG 2 cut(s) 235, 3041
BseCI ATCGAT 1 cut(s) 3162
BseDI CCNNGG 6 cut(s) 278, 633, 717, 1222, 1615, 1658
BseGI GGATG 9 cut(s) 116, 175, 295, 900, 1552, 1566, 1572, 2037, 2057
BseLI CCNNNNNNNGG 3 cut(s) 1582, 2535, 3184
BseMI GCAATG 1 cut(s) 2123
BseMII CTCAG 5 cut(s) 71, 94, 1533, 2064, 3063
BseNI ACTGG 3 cut(s) 2050, 2796, 3214
BseRI GAGGAG 3 cut(s) 869, 2898, 3041
BseSI GKGCMC 2 cut(s) 2529, 3179
BseXI GCAGC 5 cut(s) 721, 1130, 1268, 1367, 3217
BsgI GTGCAG 4 cut(s) 574, 720, 821, 2470
BshFI GGCC 7 cut(s) 479, 1586, 1614, 1657, 2312, 2527, 3039
BshVI ATCGAT 1 cut(s) 3162
BsiHKAI GWGCWC 3 cut(s) 183, 1492, 3192
BsiHKCI CYCGRG 4 cut(s) 1436, 1479, 1721, 2819
BsiSI CCGG 3 cut(s) 489, 1576, 2903
BslFI GGGAC 3 cut(s) 953, 1261, 2166
BslI CCNNNNNNNGG 3 cut(s) 1582, 2535, 3184
BsmAI GTCTC 6 cut(s) 215, 851, 1880, 2670, 2731, 2740
BsmFI GGGAC 3 cut(s) 953, 1261, 2166
BsmI GAATGC 1 cut(s) 2722
BsnI GGCC 7 cut(s) 479, 1586, 1614, 1657, 2312, 2527, 3039
Bso31I GGTCTC 1 cut(s) 2670
BsoBI CYCGRG 4 cut(s) 1436, 1479, 1721, 2819
Bsp120I GGGCCC 1 cut(s) 2525
Bsp1286I GDGCHC 6 cut(s) 183, 1492, 2529, 2820, 3179, 3192
Bsp1407I TGTACA 1 cut(s) 591
Bsp143I GATC 6 cut(s) 52, 358, 912, 1346, 2869, 3163
Bsp19I CCATGG 3 cut(s) 633, 717, 1615
BspACI CCGC 2 cut(s) 2775, 2978
BspANI GGCC 7 cut(s) 479, 1586, 1614, 1657, 2312, 2527, 3039
BspCNI CTCAG 5 cut(s) 70, 93, 1534, 2065, 3064
BspDI ATCGAT 1 cut(s) 3162
BspHI TCATGA 1 cut(s) 1044
BspLI GGNNCC 7 cut(s) 360, 1277, 2154, 2527, 2528, 2907, 3254
BspMAI CTGCAG 2 cut(s) 2236, 2300
BspMI ACCTGC 1 cut(s) 590
BspPI GGATC 4 cut(s) 47, 353, 366, 907
BspQI GCTCTTC 1 cut(s) 865
BspTI CTTAAG 1 cut(s) 2678
BspTNI GGTCTC 1 cut(s) 2670
BsrDI GCAATG 1 cut(s) 2123
BsrGI TGTACA 1 cut(s) 591
BsrI ACTGG 3 cut(s) 2050, 2796, 3214
BssECI CCNNGG 6 cut(s) 278, 633, 717, 1222, 1615, 1658
BssMI GATC 6 cut(s) 52, 358, 912, 1346, 2869, 3163
BssNI GRCGYC 1 cut(s) 2040
BssT1I CCWWGG 6 cut(s) 278, 633, 717, 1222, 1615, 1658
Bst2UI CCWGG 2 cut(s) 235, 3041
Bst4CI ACNGT 4 cut(s) 389, 591, 2439, 3282
Bst6I CTCTTC 3 cut(s) 633, 865, 2738
BstACI GRCGYC 1 cut(s) 2040
BstAFI CTTAAG 1 cut(s) 2678
BstAPI GCANNNNNTGC 1 cut(s) 2836
BstAUI TGTACA 1 cut(s) 591
BstBAI YACGTR 1 cut(s) 2443
BstC8I GCNNGC 5 cut(s) 48, 983, 1526, 1636, 1715
BstDEI CTNAG 9 cut(s) 57, 80, 408, 925, 1542, 1967, 2027, 2073, 3072
BstDSI CCRYGG 3 cut(s) 633, 717, 1615
BstF5I GGATG 9 cut(s) 116, 175, 295, 900, 1552, 1566, 1572, 2037, 2057
BstHHI GCGC 1 cut(s) 1435
BstKTI GATC 6 cut(s) 55, 361, 915, 1349, 2872, 3166
BstMAI GTCTC 6 cut(s) 215, 851, 1880, 2670, 2731, 2740
BstMBI GATC 6 cut(s) 52, 358, 912, 1346, 2869, 3163
BstMWI GCNNNNNNNGC 4 cut(s) 321, 740, 1205, 2836
BstNI CCWGG 2 cut(s) 235, 3041
BstNSI RCATGY 2 cut(s) 937, 2116
BstSCI CCNGG 4 cut(s) 233, 488, 1575, 3039
BstSFI CTRYAG 6 cut(s) 976, 1760, 2232, 2296, 2435, 3145
BstSLI GKGCMC 2 cut(s) 2529, 3179
BstSNI TACGTA 1 cut(s) 2443
BstV1I GCAGC 5 cut(s) 721, 1130, 1268, 1367, 3217
BstV2I GAAGAC 1 cut(s) 2911
BstX2I RGATCY 3 cut(s) 358, 912, 1346
BstYI RGATCY 3 cut(s) 358, 912, 1346
Bsu15I ATCGAT 1 cut(s) 3162
BsuI GTATCC 3 cut(s) 91, 496, 1573
BsuRI GGCC 7 cut(s) 479, 1586, 1614, 1657, 2312, 2527, 3039
BsuTUI ATCGAT 1 cut(s) 3162
BtgI CCRYGG 3 cut(s) 633, 717, 1615
BtgZI GCGATG 1 cut(s) 27
BtrI CACGTC 1 cut(s) 1042
BtsCI GGATG 9 cut(s) 116, 175, 295, 900, 1552, 1566, 1572, 2037, 2057
BtsI GCAGTG 2 cut(s) 458, 672
BtsIMutI CAGTG 8 cut(s) 458, 587, 653, 672, 743, 1503, 2043, 2592
BveI ACCTGC 1 cut(s) 590
Cac8I GCNNGC 5 cut(s) 48, 983, 1526, 1636, 1715
CciI TCATGA 1 cut(s) 1044
CfoI GCGC 1 cut(s) 1435
Cfr13I GGNCC 5 cut(s) 1275, 2153, 2525, 2526, 2900
ClaI ATCGAT 1 cut(s) 3162
Csp6I GTAC 7 cut(s) 371, 563, 592, 1507, 1539, 2440, 2684
CviQI GTAC 7 cut(s) 371, 563, 592, 1507, 1539, 2440, 2684
DdeI CTNAG 9 cut(s) 57, 80, 408, 925, 1542, 1967, 2027, 2073, 3072
DpnI GATC 6 cut(s) 54, 360, 914, 1348, 2871, 3165
DpnII GATC 6 cut(s) 52, 358, 912, 1346, 2869, 3163
DraI TTTAAA 1 cut(s) 501
EaeI YGGCCR 5 cut(s) 477, 1584, 1612, 1655, 2310
Eam1104I CTCTTC 3 cut(s) 633, 865, 2738
EarI CTCTTC 3 cut(s) 633, 865, 2738
Ecl136II GAGCTC 1 cut(s) 181
Eco105I TACGTA 1 cut(s) 2443
Eco130I CCWWGG 6 cut(s) 278, 633, 717, 1222, 1615, 1658
Eco24I GRGCYC 3 cut(s) 183, 2529, 2820
Eco31I GGTCTC 1 cut(s) 2670
Eco32I GATATC 2 cut(s) 1075, 1405
Eco47I GGWCC 3 cut(s) 1275, 2153, 2900
Eco53kI GAGCTC 1 cut(s) 181
Eco57I CTGAAG 5 cut(s) 1626, 1970, 2525, 2651, 2832
Eco88I CYCGRG 4 cut(s) 1436, 1479, 1721, 2819
EcoICRI GAGCTC 1 cut(s) 181
EcoO109I RGGNCCY 2 cut(s) 1275, 2526
EcoRI GAATTC 2 cut(s) 810, 1050
EcoRII CCWGG 2 cut(s) 233, 3039
EcoRV GATATC 2 cut(s) 1075, 1405
EcoT14I CCWWGG 6 cut(s) 278, 633, 717, 1222, 1615, 1658
EcoT22I ATGCAT 4 cut(s) 317, 1322, 1566, 1671
EcoT38I GRGCYC 3 cut(s) 183, 2529, 2820
ErhI CCWWGG 6 cut(s) 278, 633, 717, 1222, 1615, 1658
FaqI GGGAC 3 cut(s) 953, 1261, 2166
FauNDI CATATG 1 cut(s) 2797
FblI GTMKAC 2 cut(s) 584, 1189
Fnu4HI GCNGC 6 cut(s) 735, 1119, 1257, 1381, 2978, 3231
FokI GGATG 9 cut(s) 103, 182, 302, 907, 1559, 1559, 1573, 2044, 2064
FriOI GRGCYC 3 cut(s) 183, 2529, 2820
Fsp4HI GCNGC 6 cut(s) 735, 1119, 1257, 1381, 2978, 3231
FspBI CTAG 8 cut(s) 207, 891, 1070, 1173, 1268, 1469, 1812, 1845
GlaI GCGC 1 cut(s) 1434
GluI GCNGC 6 cut(s) 735, 1119, 1257, 1381, 2978, 3231
GsuI CTGGAG 1 cut(s) 1730
HaeIII GGCC 7 cut(s) 479, 1586, 1614, 1657, 2312, 2527, 3039
HapII CCGG 3 cut(s) 489, 1576, 2903
HhaI GCGC 1 cut(s) 1435
Hin1I GRCGYC 1 cut(s) 2040
Hin6I GCGC 1 cut(s) 1433
HinP1I GCGC 1 cut(s) 1433
HincII GTYRAC 4 cut(s) 1014, 2187, 2221, 3268
HindII GTYRAC 4 cut(s) 1014, 2187, 2221, 3268
HindIII AAGCTT 5 cut(s) 1515, 1715, 1795, 1893, 1955
HpaI GTTAAC 2 cut(s) 2187, 2221
HpaII CCGG 3 cut(s) 489, 1576, 2903
HphI GGTGA 4 cut(s) 457, 733, 2660, 2725
Hpy188I TCNGA 9 cut(s) 105, 367, 517, 1084, 1606, 2074, 2505, 3238, 3326
HpyCH4III ACNGT 4 cut(s) 389, 591, 2439, 3282
HpyCH4IV ACGT 4 cut(s) 1041, 2040, 2387, 2442
HpyF10VI GCNNNNNNNGC 4 cut(s) 321, 740, 1205, 2836
HpyF3I CTNAG 9 cut(s) 57, 80, 408, 925, 1542, 1967, 2027, 2073, 3072
HpySE526I ACGT 4 cut(s) 1041, 2040, 2387, 2442
Hsp92I GRCGYC 1 cut(s) 2040
HspAI GCGC 1 cut(s) 1433
KspAI GTTAAC 2 cut(s) 2187, 2221
Kzo9I GATC 6 cut(s) 52, 358, 912, 1346, 2869, 3163
LguI GCTCTTC 1 cut(s) 865
LmnI GCTCC 2 cut(s) 2911, 3187
Lsp1109I GCAGC 5 cut(s) 721, 1130, 1268, 1367, 3217
LweI GCATC 6 cut(s) 38, 983, 1130, 1345, 1447, 1551
MaeI CTAG 8 cut(s) 207, 891, 1070, 1173, 1268, 1469, 1812, 1845
MaeII ACGT 4 cut(s) 1041, 2040, 2387, 2442
MaeIII GTNAC 7 cut(s) 643, 1037, 2041, 2388, 2413, 2462, 2948
MalI GATC 6 cut(s) 54, 360, 914, 1348, 2871, 3165
MboI GATC 6 cut(s) 52, 358, 912, 1346, 2869, 3163
MfeI CAATTG 2 cut(s) 1194, 1593
MflI RGATCY 3 cut(s) 358, 912, 1346
MhlI GDGCHC 6 cut(s) 183, 1492, 2529, 2820, 3179, 3192
MlsI TGGCCA 4 cut(s) 479, 1614, 1657, 2312
MluNI TGGCCA 4 cut(s) 479, 1614, 1657, 2312
MlyI GAGTC 5 cut(s) 590, 1882, 2359, 3085, 3305
MmeI TCCRAC 2 cut(s) 2016, 3165
Mox20I TGGCCA 4 cut(s) 479, 1614, 1657, 2312
Mph1103I ATGCAT 4 cut(s) 317, 1322, 1566, 1671
MscI TGGCCA 4 cut(s) 479, 1614, 1657, 2312
MslI CAYNNNNRTG 2 cut(s) 2484, 3195
Msp20I TGGCCA 4 cut(s) 479, 1614, 1657, 2312
MspCI CTTAAG 1 cut(s) 2678
MspI CCGG 3 cut(s) 489, 1576, 2903
MspR9I CCNGG 4 cut(s) 235, 490, 1577, 3041
MunI CAATTG 2 cut(s) 1194, 1593
Mva1269I GAATGC 1 cut(s) 2722
MvaI CCWGG 2 cut(s) 235, 3041
MwoI GCNNNNNNNGC 4 cut(s) 321, 740, 1205, 2836
NciI CCSGG 2 cut(s) 490, 1577
NcoI CCATGG 3 cut(s) 633, 717, 1615
NdeI CATATG 1 cut(s) 2797
NdeII GATC 6 cut(s) 52, 358, 912, 1346, 2869, 3163
NlaIV GGNNCC 7 cut(s) 360, 1277, 2154, 2527, 2528, 2907, 3254
NmuCI GTSAC 3 cut(s) 643, 1037, 2041
NsiI ATGCAT 4 cut(s) 317, 1322, 1566, 1671
NspI RCATGY 2 cut(s) 937, 2116
PaeR7I CTCGAG 2 cut(s) 1479, 2819
PagI TCATGA 1 cut(s) 1044
PciI ACATGT 1 cut(s) 933
PciSI GCTCTTC 1 cut(s) 865
PctI GAATGC 1 cut(s) 2722
PfoI TCCNGGA 1 cut(s) 1575
PkrI GCNGC 6 cut(s) 736, 1120, 1258, 1382, 2979, 3232
PleI GAGTC 5 cut(s) 589, 1881, 2359, 3084, 3305
PpsI GAGTC 5 cut(s) 589, 1881, 2359, 3084, 3305
Ppu21I YACGTR 1 cut(s) 2443
PpuMI RGGWCCY 1 cut(s) 1275
PscI ACATGT 1 cut(s) 933
Psp124BI GAGCTC 1 cut(s) 183
Psp5II RGGWCCY 1 cut(s) 1275
Psp6I CCWGG 2 cut(s) 233, 3039
PspGI CCWGG 2 cut(s) 233, 3039
PspN4I GGNNCC 7 cut(s) 360, 1277, 2154, 2527, 2528, 2907, 3254
PspOMI GGGCCC 1 cut(s) 2525
PspPI GGNCC 5 cut(s) 1275, 2153, 2525, 2526, 2900
PspPPI RGGWCCY 1 cut(s) 1275
PspXI VCTCGAGB 2 cut(s) 1479, 2819
PstI CTGCAG 2 cut(s) 2236, 2300
PsuI RGATCY 3 cut(s) 358, 912, 1346
RsaI GTAC 7 cut(s) 372, 564, 593, 1508, 1540, 2441, 2685
RsaNI GTAC 7 cut(s) 371, 563, 592, 1507, 1539, 2440, 2684
RseI CAYNNNNRTG 2 cut(s) 2484, 3195
SacI GAGCTC 1 cut(s) 183
SapI GCTCTTC 1 cut(s) 865
SatI GCNGC 6 cut(s) 735, 1119, 1257, 1381, 2978, 3231
Sau3AI GATC 6 cut(s) 52, 358, 912, 1346, 2869, 3163
Sau96I GGNCC 5 cut(s) 1275, 2153, 2525, 2526, 2900
ScaI AGTACT 1 cut(s) 1540
SchI GAGTC 5 cut(s) 590, 1882, 2359, 3085, 3305
ScrFI CCNGG 4 cut(s) 235, 490, 1577, 3041
SduI GDGCHC 6 cut(s) 183, 1492, 2529, 2820, 3179, 3192
SfaNI GCATC 6 cut(s) 38, 983, 1130, 1345, 1447, 1551
SfcI CTRYAG 6 cut(s) 976, 1760, 2232, 2296, 2435, 3145
Sfr274I CTCGAG 2 cut(s) 1479, 2819
SinI GGWCC 3 cut(s) 1275, 2153, 2900
SlaI CTCGAG 2 cut(s) 1479, 2819
SmiMI CAYNNNNRTG 2 cut(s) 2484, 3195
SmlI CTYRAG 6 cut(s) 841, 1342, 1479, 1958, 2678, 2819
SmoI CTYRAG 6 cut(s) 841, 1342, 1479, 1958, 2678, 2819
SnaBI TACGTA 1 cut(s) 2443
SpeI ACTAGT 1 cut(s) 1844
SsiI CCGC 2 cut(s) 2775, 2978
SspI AATATT 3 cut(s) 2170, 2201, 2935
SspMI CTAG 8 cut(s) 207, 891, 1070, 1173, 1268, 1469, 1812, 1845
SstI GAGCTC 1 cut(s) 183
StyD4I CCNGG 4 cut(s) 233, 488, 1575, 3039
StyI CCWWGG 6 cut(s) 278, 633, 717, 1222, 1615, 1658
TaaI ACNGT 4 cut(s) 389, 591, 2439, 3282
TaiI ACGT 4 cut(s) 1044, 2043, 2390, 2445
TaqI TCGA 5 cut(s) 183, 225, 1480, 2820, 3162
TatI WGTACW 3 cut(s) 562, 591, 1538
TauI GCSGC 1 cut(s) 2980
TscAI CASTG 8 cut(s) 458, 594, 660, 679, 750, 1503, 2050, 2592
TseFI GTSAC 3 cut(s) 643, 1037, 2041
TseI GCWGC 5 cut(s) 734, 1118, 1256, 1380, 3230
Tsp45I GTSAC 3 cut(s) 643, 1037, 2041
TspDTI ATGAA 9 cut(s) 30, 306, 1413, 1439, 1686, 1806, 2496, 2567, 2770
TspRI CASTG 8 cut(s) 458, 594, 660, 679, 750, 1503, 2050, 2592
Vha464I CTTAAG 1 cut(s) 2678
VpaK11BI GGWCC 3 cut(s) 1275, 2153, 2900
XbaI TCTAGA 1 cut(s) 1069
XceI RCATGY 2 cut(s) 937, 2116
XhoI CTCGAG 2 cut(s) 1479, 2819
XmiI GTMKAC 2 cut(s) 584, 1189
XspI CTAG 8 cut(s) 207, 891, 1070, 1173, 1268, 1469, 1812, 1845
ZraI GACGTC 1 cut(s) 2041
ZrmI AGTACT 1 cut(s) 1540
Zsp2I ATGCAT 4 cut(s) 317, 1322, 1566, 1671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.