Rmu_sc0000595.1_g000006

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000595.1
OG0000085
Physical Location & Seq
Reverse (-)
33824 .. 40205
6382 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000595.1_g000006.1.cds

Sequence Viewer

Length: 5052 bp
atggcctggttctctcgagaaactgatgcttctcttccttcatcagctgaaccactatcagcagctggtgggtggaaatatgacgtgttcttgagcttcatgggtcctgacactcgaaggggttttacatcatatttatacgatgcgctgcagagcaggggaattaaaacattcatggacgatcaagatcttcaagtaagtgatgctatttctccatttctcctaaaggcaattgaagaatcgaggttcgcaattgttattttgtcgcaaaattatgcttcttctacttggtgcttggaggaacttacaaagatctgtgagtgtatgaaagacagttacagaattcttccactattttattacgtggagccctctgatgttcggtatcagaaaagaagtttcgcagaagctttctctgcacatgaaatctctgggcgacatagatcagagaaggtccagcagtggagagctgctttaaacaaagtggccaatttctctgggtggacaacaaaggattttgcgtccgagagagaacttctgcaaaatattgtggaatttgtagcgagtaaagtactccaagcacctattgaaatcgagtctgcaatgtggcttaaagatgaaccggctgatcctcgatggaagtatgatgtgtttctgagtttcaagggtgaagacactcgcaagggttttctatcccatttataccatgaactgcagaacacgggagtgattaaaacattcaaggatgatttagagcctggagaaggggtacatatttctccgactttcctaaaggcaattgaagaatcaaggtttgccattgttgttctgacgccaaactatgcttcttctccatggtgtttggaggaacttacgaacatttttcactgcatggaagaccagcaaagaattctaccaattttttatcatgtggaaccttctgatgtacgatatcaaaagaagagttttcagatagctttcacaaagcataaaaaaaggcaagacgaggagaaagtgaagcaatggagtagtgctttaaaaaaagtggccaatctctctggctgggattcaaggaattataagactgaaagacaacttgtcgaagacattgtggcatttgtctactataaagtacaagcacaccatatcgaatttgagtctgttgaacataagaagcctactgacgttgagtctttaataccgattgaagattctgaagcaacaacacaagccacagaccaaactttggccccgagaagccaaagacactgttcatttcgtccttcagctcctcggtggaagcatgaagtgtttttgagcttcagggatgtagacgcctgcaagggtattacatccgagatatatgataatttgcagaacatcagaggagttaaaacattcatgaacaaccaagagtttcaagtaggtgaccttacttctcccagattcctaactacaattgaagaatcaaggtttgcgatagttgttttgtcgcaaaactatgcttcctctacctggtgtttggaggaacttacaacaattgttcaatgcatgaaagataatgatagagttctgccacttttttatcaagtggatccatctgctgtgcggtgtcagagagggagttttgaagttgctttcaaaaatcacgagtctacggaagacacggcgaaggtggagcagtggagaaatgctctaaaaaaggtggccgatctccctggatgggaatcccacgaatatgggtctgacaaagaacttgtcgaagacattgtgaaacttgtgtggggtaaagtccttcaagtacgtattgataatgagtcgaccatacgacaacccaaggacatgacttcctcaagatctcttgcttcagctaaacccgctcctcgatggaagtatgatgtttttttgaatttcaggggtgaagacacccgtgagaatattctttcccatttataccgtgaattatcggacacgagaggagttaaaacatttaaggatgaccaaaaccttgagcaagggacggccatttctccaaatcttctaatggcaatagaggaatctaagtttgcagttgtcgttctatctgcaaattatgcttcttctacgtggtgtttagatgaacttatacatatttttaaatgcatggaaggcaagaacacaattctgcctattttttatcatgtggatccctctcatgtccagaaacaagaggagagttttaaaaaagctttcgagcagcatgaaaactctggtcaagatgaacagaagatgcaacaatggagagaggctttaaaaaaagtggccactgtgtctggatggcattcaaaaaaatataagtccgaaagcgaacttgttgaacacgttgggaaatttgtgtggagtaaagtgattcaagttgaagttgactctacaatgtccactgcagattttgaagcatttagagcaacaagacaaggcatggaacaggtcatgaaggcgttaaaaaatgataaggtctacgtgattggggtctatggaaagggaggagtagggaagacaaccatggtgaaacatgtaactgctcaagcccaaaagattgggctgcttaatcatgttttcatggtttccctatcccaaagtcctgatctgagaaaaattcaaggcaaattcgcagatattctggggttgaaattccaggaagagacagatattgaaaaagccacaagtctaaagaagaggatcatgggaggacataagatccttatagtcttggacaacatttggaacagtatagatttgtctagcataggaattcccagctccagcgagcttcaaagatgcaatgctaaagtcctaatcaccacaaggaaaattgaagtatgtcaggccatggggtgccaagaaaaaatcaatctcactatgctatcagaggcagattctttgactttattcatgaggcaatcaggaaagtctttagaatcaaaagatttctatgatgcagcaaggaaggtagctaaacaatgtgctggtctaccaattgcgctaaaagcagttgcgaaagcccttggagataaagttgatttgaatgaatggaaaaaagcagctcgacgactgaaaaaatcccgaacagccaaccttgatgatgagggagatgggtttaattgtataaagttgagctatgattacttgaaaggggagtatgtaaagtcctgcttcttgctttgttgtctattcccagacgatgatgatatccgaatagcagacttgatggagtatgggtacggtattggattgtttcaagattccagcacaatgaaagaggccagagccacagcttatttggcggtcatataccttaaagcatgtaacttgcttatggatggtgcagaggatggatgcataaggatgcatgatgtcattcggaatatggccatctcaatattgcataaaaatggtcatcatttctttccaccaggaaagacagaagatggccatcatttttcttcaccagggaaaaaagatgatggccatccttcttttaaaccagaaaaagaagaaaaagatctccatcattttttgataaaagctggttgtcaattaaaagaatggccaatggatgcagatgaaagctactcaggaatttcagtaatgagaaatgaaattctcaagctgcctgaagaattgatatgttcaaaactgcaaattttattgttacaaggtaatgctgatataaatgacatcccagatacttttatccaaagtgtgaattcaatgagggtgttagatctgagcgacaccagaatttcttcactaccaccatcattcagtctcctgaaaaatctccacactttgtatctagatggttgccagtcgctaattgatgtatctgcactcagagaattcagattacttgagattcttagtatgagacagtgttgttttaaggaattgccaatggaaataggacaagtgagtgatctgaggatattggacatcactagtgaatttgttaatacagttcctcctaatgtggtgtcaagattgcacagattagaagaattgtacatgcaatgcaaatttatggactggagcagtaacggtaagggagcaggaaatgctggttttgatgagttgactggtttgtcacatttgtacattttaaaggttaacatatctgatccagactgcttgcctaaaattgttgagtgcagcccaaattgggaaatctttgatatacgtatcagcagagaaacaatacctagagattactcttcgaagccatactttaaatgtgatcattcaagaatcttgactctcagtacagcaatcaataccttaccagattggtttatcatgtcggttacaaagaaagcagatagtctacaatacatagagtgtaatgaattaaataacatgcttgtggagcatgaacgtgggatattacatggactaaagtatcttgctgtaattgggcgccatgagaaattggaagagttgatgaacacaaaaacatgtgttccagttccaaatgagcctgtgttcgagagcatggaagagttgcatctggctggtttgaattgcttgaaggagttatgtgttggtgaactaccgccccagtcactctgcaatattaagttactcaaagtggaagcttgtcctaacttggtgaatgcactcttaccatcaatcttctgtaatcttactattttggtggttaggtgttgcatgaaactgaaaagtctcttcacaccagatgtagctcgatgtcttttgcaattggaagacctttcagtagctcaatgtgcttgcttgaacagagttattgaagcaagtgaagaaacagtagagatgaagatggcttttccaaaattgaagaaattagttttgtataatcttccacagctcacgtggttctactgcacgggaaatgctactatttattgcccttcattgcaacagttgtatgttcaggactgtcccgagtttttgacctctacttctgactttcagacctggaagcaagttgaagtcaatgatttacggcatctgagaacagacaacgatgagatgtaa

Protein Analysis

1683

Amino Acids

192.03

Weight (kDa)

5.91

Isoelectric Point (pI)

48.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1080
AasI GACNNNNNNGTC 1 cut(s) 4120
AccB1I GGYRCC 2 cut(s) 2874, 4452
AccB7I CCANNNNNTGG 1 cut(s) 1246
AccBSI CCGCTC 1 cut(s) 1880
AccI GTMKAC 7 cut(s) 1122, 1332, 1655, 1820, 2496, 3010, 4360
AciI CCGC 4 cut(s) 1609, 1878, 3323, 4589
AclWI GGATC 8 cut(s) 623, 1589, 1602, 2180, 2193, 2726, 2731, 4151
AcoI YGGCCR 9 cut(s) 486, 1047, 1707, 2022, 2301, 3408, 3469, 3505, 3587
AcuI CTGAAG 5 cut(s) 1236, 1269, 1306, 1851, 3675
AcvI CACGTG 1 cut(s) 4887
AcyI GRCGYC 3 cut(s) 833, 1335, 4453
AfaI GTAC 9 cut(s) 573, 771, 948, 1134, 1803, 3260, 4043, 4133, 4300
AfiI CCNNNNNNNGG 5 cut(s) 764, 1246, 1515, 1723, 4198
AflIII ACRYGT 4 cut(s) 84, 2359, 2551, 4490
AhdI GACNNNNNGTC 2 cut(s) 1097, 1189
AhlI ACTAGT 1 cut(s) 3977
AjiI CACGTC 1 cut(s) 85
AjnI CCWGG 8 cut(s) 5, 757, 1514, 1717, 2673, 3451, 3487, 4991
AjuI GAANNNNNNNTTGG 2 cut(s) 829, 861
AleI CACNNNNGTG 1 cut(s) 725
Alw26I GTCTC 4 cut(s) 2675, 3812, 3901, 4724
AlwI GGATC 8 cut(s) 623, 1589, 1602, 2180, 2193, 2726, 2731, 4151
AlwNI CAGNNNCTG 1 cut(s) 65
Ama87I CYCGRG 3 cut(s) 15, 1252, 4958
ApeKI GCWGC 9 cut(s) 62, 148, 470, 2236, 2581, 2978, 3080, 3649, 4188
ArsI GACNNNNNNTTYG 2 cut(s) 1743, 1775
Asp700I GAANNNNTTC 1 cut(s) 3784
AspLEI GCGC 3 cut(s) 148, 3022, 4455
AspS9I GGNCC 3 cut(s) 104, 454, 1249
AsuHPI GGTGA 8 cut(s) 680, 1439, 1931, 2557, 2830, 3477, 4592, 4657
AsuII TTCGAA 1 cut(s) 4253
AvaI CYCGRG 3 cut(s) 15, 1252, 4958
AvaII GGWCC 2 cut(s) 104, 454
BaeI ACNNNNGTAYC 2 cut(s) 4418, 4451
BalI TGGCCA 7 cut(s) 488, 1049, 2303, 3410, 3471, 3507, 3589
BamHI GGATCC 2 cut(s) 1594, 2185
BanI GGYRCC 2 cut(s) 2874, 4452
BanII GRGCYC 1 cut(s) 372
BarI GAAGNNNNNNTAC 2 cut(s) 109, 141
BauI CACGAG 2 cut(s) 1649, 1972
BbrPI CACGTG 1 cut(s) 4887
BbsI GAAGAC 8 cut(s) 678, 903, 1110, 1668, 1770, 1929, 2540, 4767
BbvI GCAGC 9 cut(s) 74, 135, 457, 2248, 2568, 2990, 3092, 3636, 4200
BceAI ACGGC 3 cut(s) 1683, 2037, 5036
BciT130I CCWGG 8 cut(s) 7, 759, 1516, 1719, 2675, 3453, 3489, 4993
BclI TGATCA 1 cut(s) 4273
BcoDI GTCTC 4 cut(s) 2675, 3812, 3901, 4724
BcuI ACTAGT 1 cut(s) 3977
BfaI CTAG 4 cut(s) 2781, 3836, 3978, 4239
BfmI CTRYAG 3 cut(s) 149, 713, 2421
BfoI RGCGCY 1 cut(s) 4456
BglII AGATCT 5 cut(s) 187, 312, 1856, 3541, 3763
BisI GCNGC 9 cut(s) 63, 149, 471, 2237, 2582, 2979, 3081, 3650, 4189
BlsI GCNGC 9 cut(s) 64, 150, 472, 2238, 2583, 2980, 3082, 3651, 4190
BmcAI AGTACT 1 cut(s) 573
Bme1390I CCNGG 8 cut(s) 7, 759, 1516, 1719, 2675, 3453, 3489, 4993
Bme18I GGWCC 2 cut(s) 104, 454
BmeRI GACNNNNNGTC 2 cut(s) 1097, 1189
BmeT110I CYCGRG 3 cut(s) 15, 1252, 4958
BmgBI CACGTC 1 cut(s) 85
BmgT120I GGNCC 3 cut(s) 104, 454, 1249
BmiI GGNNCC 8 cut(s) 105, 369, 936, 1251, 1596, 2187, 2876, 4454
BmrFI CCNGG 8 cut(s) 7, 759, 1516, 1719, 2675, 3453, 3489, 4993
BmrI ACTGGG 1 cut(s) 4588
BmuI ACTGGG 1 cut(s) 4588
BpiI GAAGAC 8 cut(s) 678, 903, 1110, 1668, 1770, 1929, 2540, 4767
BplI GAGNNNNNCTC 2 cut(s) 1678, 1710
BpmI CTGGAG 3 cut(s) 780, 2785, 4087
Bpu14I TTCGAA 1 cut(s) 4253
BpuEI CTTGAG 6 cut(s) 112, 1837, 2030, 2547, 3629, 3911
BsaAI YACGTR 6 cut(s) 364, 1805, 2106, 2500, 4217, 4887
BsaBI GATNNNNATC 5 cut(s) 186, 1726, 3471, 3507, 3546
BsaHI GRCGYC 3 cut(s) 833, 1335, 4453
BsaJI CCNNGG 8 cut(s) 854, 1292, 1717, 1836, 2541, 2868, 3043, 3488
BsaXI ACNNNNNCTCC 8 cut(s) 753, 783, 3039, 3069, 3242, 3272, 3985, 4015
Bsc4I CCNNNNNNNGG 5 cut(s) 764, 1246, 1515, 1723, 4198
Bse118I RCCGGY 1 cut(s) 622
Bse1I ACTGG 5 cut(s) 3847, 4070, 4120, 4499, 4594
Bse3DI GCAATG 5 cut(s) 609, 1028, 2827, 4055, 4928
Bse8I GATNNNNATC 5 cut(s) 186, 1726, 3471, 3507, 3546
BseBI CCWGG 8 cut(s) 7, 759, 1516, 1719, 2675, 3453, 3489, 4993
BseDI CCNNGG 8 cut(s) 854, 1292, 1717, 1836, 2541, 2868, 3043, 3488
BseJI GATNNNNATC 5 cut(s) 186, 1726, 3471, 3507, 3546
BseLI CCNNNNNNNGG 5 cut(s) 764, 1246, 1515, 1723, 4198
BseMI GCAATG 5 cut(s) 609, 1028, 2827, 4055, 4928
BseMII CTCAG 8 cut(s) 647, 2618, 3627, 3758, 3886, 3950, 4309, 5018
BseNI ACTGG 5 cut(s) 3847, 4070, 4120, 4499, 4594
BseRI GAGGAG 7 cut(s) 1022, 1281, 1401, 1872, 1992, 2225, 2538
BseXI GCAGC 9 cut(s) 74, 135, 457, 2248, 2568, 2990, 3092, 3636, 4200
BseYI CCCAGC 2 cut(s) 1062, 2795
BsgI GTGCAG 5 cut(s) 402, 3384, 3852, 4207, 4882
BshNI GGYRCC 2 cut(s) 2874, 4452
BsiHKCI CYCGRG 3 cut(s) 15, 1252, 4958
BsiSI CCGG 1 cut(s) 623
BslFI GGGAC 2 cut(s) 2032, 4941
BslI CCNNNNNNNGG 5 cut(s) 764, 1246, 1515, 1723, 4198
BsmAI GTCTC 4 cut(s) 2675, 3812, 3901, 4724
BsmFI GGGAC 2 cut(s) 2032, 4941
BsmI GAATGC 2 cut(s) 2320, 4654
BsoBI CYCGRG 3 cut(s) 15, 1252, 4958
Bsp119I TTCGAA 1 cut(s) 4253
Bsp1286I GDGCHC 1 cut(s) 372
Bsp1407I TGTACA 2 cut(s) 4041, 4131
Bsp19I CCATGG 3 cut(s) 854, 2541, 2868
BspACI CCGC 4 cut(s) 1609, 1878, 3323, 4589
BspCNI CTCAG 8 cut(s) 648, 2619, 3626, 3759, 3885, 3951, 4308, 5019
BspHI TCATGA 3 cut(s) 1401, 2469, 2931
BspLI GGNNCC 8 cut(s) 105, 369, 936, 1251, 1596, 2187, 2876, 4454
BspMAI CTGCAG 3 cut(s) 153, 717, 2425
BspPI GGATC 8 cut(s) 623, 1589, 1602, 2180, 2193, 2726, 2731, 4151
BspT104I TTCGAA 1 cut(s) 4253
BspT107I GGYRCC 2 cut(s) 2874, 4452
BsrBI CCGCTC 1 cut(s) 1880
BsrDI GCAATG 5 cut(s) 609, 1028, 2827, 4055, 4928
BsrFI RCCGGY 1 cut(s) 622
BsrGI TGTACA 2 cut(s) 4041, 4131
BsrI ACTGG 5 cut(s) 3847, 4070, 4120, 4499, 4594
BssAI RCCGGY 1 cut(s) 622
BssECI CCNNGG 8 cut(s) 854, 1292, 1717, 1836, 2541, 2868, 3043, 3488
BssNI GRCGYC 3 cut(s) 833, 1335, 4453
BssSI CACGAG 2 cut(s) 1649, 1972
BssT1I CCWWGG 5 cut(s) 854, 1836, 2541, 2868, 3043
Bst2BI CACGAG 2 cut(s) 1649, 1972
Bst2UI CCWGG 8 cut(s) 7, 759, 1516, 1719, 2675, 3453, 3489, 4993
Bst6I CTCTTC 8 cut(s) 39, 956, 2673, 2708, 4255, 4464, 4527, 4727
BstACI GRCGYC 3 cut(s) 833, 1335, 4453
BstAPI GCANNNNNTGC 2 cut(s) 2093, 4094
BstAUI TGTACA 2 cut(s) 4041, 4131
BstBAI YACGTR 6 cut(s) 364, 1805, 2106, 2500, 4217, 4887
BstBI TTCGAA 1 cut(s) 4253
BstC8I GCNNGC 4 cut(s) 1339, 2807, 4169, 4786
BstDSI CCRYGG 3 cut(s) 854, 2541, 2868
BstEII GGTNACC 1 cut(s) 1427
BstENI CCTNNNNNAGG 1 cut(s) 762
BstH2I RGCGCY 1 cut(s) 4456
BstHHI GCGC 3 cut(s) 148, 3022, 4455
BstMAI GTCTC 4 cut(s) 2675, 3812, 3901, 4724
BstNI CCWGG 8 cut(s) 7, 759, 1516, 1719, 2675, 3453, 3489, 4993
BstNSI RCATGY 5 cut(s) 2555, 3345, 4048, 4396, 4494
BstPI GGTNACC 1 cut(s) 1427
BstSCI CCNGG 8 cut(s) 5, 757, 1514, 1717, 2673, 3451, 3487, 4991
BstSFI CTRYAG 3 cut(s) 149, 713, 2421
BstSNI TACGTA 2 cut(s) 1805, 4217
BstV1I GCAGC 9 cut(s) 74, 135, 457, 2248, 2568, 2990, 3092, 3636, 4200
BstV2I GAAGAC 8 cut(s) 678, 903, 1110, 1668, 1770, 1929, 2540, 4767
BstX2I RGATCY 8 cut(s) 187, 312, 1594, 1856, 2185, 2736, 3541, 3763
BstXI CCANNNNNNTGG 2 cut(s) 1739, 2576
BstYI RGATCY 8 cut(s) 187, 312, 1594, 1856, 2185, 2736, 3541, 3763
BtgI CCRYGG 3 cut(s) 854, 2541, 2868
BtrI CACGTC 1 cut(s) 85
BtsI GCAGTG 4 cut(s) 467, 886, 1688, 2418
BtsIMutI CAGTG 7 cut(s) 467, 886, 1267, 1688, 2304, 2418, 3917
Cac8I GCNNGC 4 cut(s) 1339, 2807, 4169, 4786
CaiI CAGNNNCTG 1 cut(s) 65
CciI TCATGA 3 cut(s) 1401, 2469, 2931
CfoI GCGC 3 cut(s) 148, 3022, 4455
Cfr10I RCCGGY 1 cut(s) 622
Cfr13I GGNCC 3 cut(s) 104, 454, 1249
CseI GACGC 3 cut(s) 510, 841, 1343
CsiI ACCWGGT 1 cut(s) 1514
Csp6I GTAC 9 cut(s) 572, 770, 947, 1133, 1802, 3259, 4042, 4132, 4299
CviQI GTAC 9 cut(s) 572, 770, 947, 1133, 1802, 3259, 4042, 4132, 4299
DinI GGCGCC 1 cut(s) 4454
DraI TTTAAA 8 cut(s) 477, 1038, 2137, 2221, 2292, 3520, 4140, 4267
DrdI GACNNNNNNGTC 1 cut(s) 4120
DriI GACNNNNNGTC 2 cut(s) 1097, 1189
DseDI GACNNNNNNGTC 1 cut(s) 4120
EaeI YGGCCR 9 cut(s) 486, 1047, 1707, 2022, 2301, 3408, 3469, 3505, 3587
Eam1104I CTCTTC 8 cut(s) 39, 956, 2673, 2708, 4255, 4464, 4527, 4727
Eam1105I GACNNNNNGTC 2 cut(s) 1097, 1189
EarI CTCTTC 8 cut(s) 39, 956, 2673, 2708, 4255, 4464, 4527, 4727
Eco105I TACGTA 2 cut(s) 1805, 4217
Eco130I CCWWGG 5 cut(s) 854, 1836, 2541, 2868, 3043
Eco24I GRGCYC 1 cut(s) 372
Eco32I GATATC 2 cut(s) 953, 3229
Eco47I GGWCC 2 cut(s) 104, 454
Eco57I CTGAAG 5 cut(s) 1236, 1269, 1306, 1851, 3675
Eco72I CACGTG 1 cut(s) 4887
Eco88I CYCGRG 3 cut(s) 15, 1252, 4958
Eco91I GGTNACC 1 cut(s) 1427
EcoNI CCTNNNNNAGG 1 cut(s) 762
EcoO109I RGGNCCY 1 cut(s) 104
EcoO65I GGTNACC 1 cut(s) 1427
EcoRI GAATTC 5 cut(s) 342, 909, 2790, 3745, 3878
EcoRII CCWGG 8 cut(s) 5, 757, 1514, 1717, 2673, 3451, 3487, 4991
EcoRV GATATC 2 cut(s) 953, 3229
EcoT14I CCWWGG 5 cut(s) 854, 1836, 2541, 2868, 3043
EcoT22I ATGCAT 4 cut(s) 1553, 2144, 3380, 3390
EcoT38I GRGCYC 1 cut(s) 372
EgeI GGCGCC 1 cut(s) 4454
EheI GGCGCC 1 cut(s) 4454
ErhI CCWWGG 5 cut(s) 854, 1836, 2541, 2868, 3043
FalI AAGNNNNNCTT 4 cut(s) 3176, 3208, 4247, 4279
FaqI GGGAC 2 cut(s) 2032, 4941
FauI CCCGC 1 cut(s) 1885
FbaI TGATCA 1 cut(s) 4273
FblI GTMKAC 7 cut(s) 1122, 1332, 1655, 1820, 2496, 3010, 4360
Fnu4HI GCNGC 9 cut(s) 63, 149, 471, 2237, 2582, 2979, 3081, 3650, 4189
FriOI GRGCYC 1 cut(s) 372
Fsp4HI GCNGC 9 cut(s) 63, 149, 471, 2237, 2582, 2979, 3081, 3650, 4189
FspBI CTAG 4 cut(s) 2781, 3836, 3978, 4239
GlaI GCGC 3 cut(s) 147, 3021, 4454
GluI GCNGC 9 cut(s) 63, 149, 471, 2237, 2582, 2979, 3081, 3650, 4189
GsaI CCCAGC 2 cut(s) 1066, 2799
GsuI CTGGAG 3 cut(s) 780, 2785, 4087
HaeII RGCGCY 1 cut(s) 4456
HapII CCGG 1 cut(s) 623
HgaI GACGC 3 cut(s) 510, 841, 1343
HhaI GCGC 3 cut(s) 148, 3022, 4455
Hin1I GRCGYC 3 cut(s) 833, 1335, 4453
Hin6I GCGC 3 cut(s) 146, 3020, 4453
HinP1I GCGC 3 cut(s) 146, 3020, 4453
HincII GTYRAC 4 cut(s) 1821, 2404, 4113, 4147
HindII GTYRAC 4 cut(s) 1821, 2404, 4113, 4147
HindIII AAGCTT 3 cut(s) 408, 2226, 4629
HpaI GTTAAC 1 cut(s) 4147
HpaII CCGG 1 cut(s) 623
HphI GGTGA 8 cut(s) 680, 1439, 1931, 2557, 2830, 3477, 4592, 4657
Hpy99I CGWCG 1 cut(s) 3090
Hsp92I GRCGYC 3 cut(s) 833, 1335, 4453
HspAI GCGC 3 cut(s) 146, 3020, 4453
KasI GGCGCC 1 cut(s) 4452
Ksp22I TGATCA 1 cut(s) 4273
KspAI GTTAAC 1 cut(s) 4147
LmnI GCTCC 8 cut(s) 367, 1294, 1678, 1885, 2804, 4068, 4085, 4402
Lsp1109I GCAGC 9 cut(s) 74, 135, 457, 2248, 2568, 2990, 3092, 3636, 4200
MabI ACCWGGT 1 cut(s) 1514
MaeI CTAG 4 cut(s) 2781, 3836, 3978, 4239
MbiI CCGCTC 1 cut(s) 1880
MfeI CAATTG 7 cut(s) 231, 252, 798, 1458, 1539, 3015, 4754
MflI RGATCY 8 cut(s) 187, 312, 1594, 1856, 2185, 2736, 3541, 3763
MhlI GDGCHC 1 cut(s) 372
MlsI TGGCCA 7 cut(s) 488, 1049, 2303, 3410, 3471, 3507, 3589
MluNI TGGCCA 7 cut(s) 488, 1049, 2303, 3410, 3471, 3507, 3589
Mly113I GGCGCC 1 cut(s) 4453
MlyI GAGTC 7 cut(s) 605, 1166, 1199, 1661, 1826, 2399, 4285
MmeI TCCRAC 1 cut(s) 806
Mox20I TGGCCA 7 cut(s) 488, 1049, 2303, 3410, 3471, 3507, 3589
Mph1103I ATGCAT 4 cut(s) 1553, 2144, 3380, 3390
MroXI GAANNNNTTC 1 cut(s) 3784
MscI TGGCCA 7 cut(s) 488, 1049, 2303, 3410, 3471, 3507, 3589
MslI CAYNNNNRTG 6 cut(s) 725, 1737, 3383, 3429, 4397, 4410
Msp20I TGGCCA 7 cut(s) 488, 1049, 2303, 3410, 3471, 3507, 3589
MspA1I CMGCKG 2 cut(s) 47, 65
MspI CCGG 1 cut(s) 623
MspR9I CCNGG 8 cut(s) 7, 759, 1516, 1719, 2675, 3453, 3489, 4993
MunI CAATTG 7 cut(s) 231, 252, 798, 1458, 1539, 3015, 4754
Mva1269I GAATGC 2 cut(s) 2320, 4654
MvaI CCWGG 8 cut(s) 7, 759, 1516, 1719, 2675, 3453, 3489, 4993
NarI GGCGCC 1 cut(s) 4453
NcoI CCATGG 3 cut(s) 854, 2541, 2868
NlaIV GGNNCC 8 cut(s) 105, 369, 936, 1251, 1596, 2187, 2876, 4454
NmuCI GTSAC 3 cut(s) 1427, 4122, 4596
NsiI ATGCAT 4 cut(s) 1553, 2144, 3380, 3390
NspI RCATGY 5 cut(s) 2555, 3345, 4048, 4396, 4494
NspV TTCGAA 1 cut(s) 4253
OliI CACNNNNGTG 1 cut(s) 725
PaeR7I CTCGAG 1 cut(s) 15
PagI TCATGA 3 cut(s) 1401, 2469, 2931
PciI ACATGT 2 cut(s) 2551, 4490
PctI GAATGC 2 cut(s) 2320, 4654
PdmI GAANNNNTTC 1 cut(s) 3784
PflMI CCANNNNNTGG 1 cut(s) 1246
PfoI TCCNGGA 1 cut(s) 2673
PkrI GCNGC 9 cut(s) 64, 150, 472, 2238, 2583, 2980, 3082, 3651, 4190
PleI GAGTC 7 cut(s) 604, 1165, 1198, 1660, 1825, 2399, 4285
PluTI GGCGCC 1 cut(s) 4456
PmaCI CACGTG 1 cut(s) 4887
PmlI CACGTG 1 cut(s) 4887
PpsI GAGTC 7 cut(s) 604, 1165, 1198, 1660, 1825, 2399, 4285
Ppu21I YACGTR 6 cut(s) 364, 1805, 2106, 2500, 4217, 4887
PpuMI RGGWCCY 1 cut(s) 104
PscI ACATGT 2 cut(s) 2551, 4490
PsiI TTATAA 1 cut(s) 1080
Psp5II RGGWCCY 1 cut(s) 104
Psp6I CCWGG 8 cut(s) 5, 757, 1514, 1717, 2673, 3451, 3487, 4991
PspCI CACGTG 1 cut(s) 4887
PspEI GGTNACC 1 cut(s) 1427
PspFI CCCAGC 2 cut(s) 1062, 2795
PspGI CCWGG 8 cut(s) 5, 757, 1514, 1717, 2673, 3451, 3487, 4991
PspN4I GGNNCC 8 cut(s) 105, 369, 936, 1251, 1596, 2187, 2876, 4454
PspPI GGNCC 3 cut(s) 104, 454, 1249
PspPPI RGGWCCY 1 cut(s) 104
PstI CTGCAG 3 cut(s) 153, 717, 2425
PstNI CAGNNNCTG 1 cut(s) 65
PsuI RGATCY 8 cut(s) 187, 312, 1594, 1856, 2185, 2736, 3541, 3763
PvuII CAGCTG 2 cut(s) 47, 65
RsaI GTAC 9 cut(s) 573, 771, 948, 1134, 1803, 3260, 4043, 4133, 4300
RsaNI GTAC 9 cut(s) 572, 770, 947, 1133, 1802, 3259, 4042, 4132, 4299
RseI CAYNNNNRTG 6 cut(s) 725, 1737, 3383, 3429, 4397, 4410
SalI GTCGAC 1 cut(s) 1819
SatI GCNGC 9 cut(s) 63, 149, 471, 2237, 2582, 2979, 3081, 3650, 4189
Sau96I GGNCC 3 cut(s) 104, 454, 1249
ScaI AGTACT 1 cut(s) 573
SchI GAGTC 7 cut(s) 605, 1166, 1199, 1661, 1826, 2399, 4285
ScrFI CCNGG 8 cut(s) 7, 759, 1516, 1719, 2675, 3453, 3489, 4993
SduI GDGCHC 1 cut(s) 372
SexAI ACCWGGT 1 cut(s) 1514
SfcI CTRYAG 3 cut(s) 149, 713, 2421
SfoI GGCGCC 1 cut(s) 4454
Sfr274I CTCGAG 1 cut(s) 15
SfuI TTCGAA 1 cut(s) 4253
SinI GGWCC 2 cut(s) 104, 454
SlaI CTCGAG 1 cut(s) 15
SmiMI CAYNNNNRTG 6 cut(s) 725, 1737, 3383, 3429, 4397, 4410
SmlI CTYRAG 7 cut(s) 15, 91, 1852, 2009, 2562, 3644, 3890
SmoI CTYRAG 7 cut(s) 15, 91, 1852, 2009, 2562, 3644, 3890
SnaBI TACGTA 2 cut(s) 1805, 4217
SpeI ACTAGT 1 cut(s) 3977
SsiI CCGC 4 cut(s) 1609, 1878, 3323, 4589
SspDI GGCGCC 1 cut(s) 4452
SspI AATATT 4 cut(s) 547, 1939, 3420, 4609
SspMI CTAG 4 cut(s) 2781, 3836, 3978, 4239
StyD4I CCNGG 8 cut(s) 5, 757, 1514, 1717, 2673, 3451, 3487, 4991
StyI CCWWGG 5 cut(s) 854, 1836, 2541, 2868, 3043
TatI WGTACW 5 cut(s) 571, 1132, 4041, 4131, 4298
TscAI CASTG 7 cut(s) 467, 893, 1274, 1688, 2311, 2425, 3917
TseFI GTSAC 3 cut(s) 1427, 4122, 4596
TseI GCWGC 9 cut(s) 62, 148, 470, 2236, 2581, 2978, 3080, 3649, 4188
Tsp45I GTSAC 3 cut(s) 1427, 4122, 4596
TspGWI ACGGA 1 cut(s) 1673
TspRI CASTG 7 cut(s) 467, 893, 1274, 1688, 2311, 2425, 3917
Van91I CCANNNNNTGG 1 cut(s) 1246
VpaK11BI GGWCC 2 cut(s) 104, 454
XagI CCTNNNNNAGG 1 cut(s) 762
XbaI TCTAGA 1 cut(s) 3835
XceI RCATGY 5 cut(s) 2555, 3345, 4048, 4396, 4494
XcmI CCANNNNNNNNNTGG 2 cut(s) 3316, 4884
XhoI CTCGAG 1 cut(s) 15
XmiI GTMKAC 7 cut(s) 1122, 1332, 1655, 1820, 2496, 3010, 4360
XmnI GAANNNNTTC 1 cut(s) 3784
XspI CTAG 4 cut(s) 2781, 3836, 3978, 4239
ZrmI AGTACT 1 cut(s) 573
Zsp2I ATGCAT 4 cut(s) 1553, 2144, 3380, 3390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.