Rroxscaffold_3G00237530

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000085
Physical Location & Seq
Forward (+)
25202247 .. 25211054
8808 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00237530.1

Sequence Viewer

Length: 2739 bp
ATGGCCCTGCTTAGAGCCTCAGCTGAACCGGCTTCTCCTCGTCGGTGGAAGCATGATGTGTTTTTAAGTTTCAGGGGTGAAGAAACCCGCAAGGGCATTGTTTCCCATTTATACGATGAGCTGCAAAACAGGAGAGGGATTAAAACATTCAAGGATGATCAGCAGCTTGAAATAGGGACAGCTATTTCTCCAAGTCTCCTAACGGCAATCCAAGAATCATGGTTTGCCATCATTGTTCTCTCGCCAAATTATGCTTCTTCTTCTTGGTGTTTGGATGAACTCACACACATTTTTCAATGCATGGAAGACAAGAACAGAATTCTGCCTCTCTTTTATCACGTTGATCCCTCTGACGTACGCCATCAAAGGGGAAGTTTTCAAGTAGCTTTCACTAAACATGAATCTAGGCAAGACATGGACAAGGTCAAGCAGTGGAGAGATGCTTTAAATAAAGTTGCCTATCTCTGTGGCTGGGATGCCAAGAATTATGTATCCGAAAGAGAGCTTGTCAAAAGCATTGTGACATTTGTGTGCAGCAAAGTACTTCAAGTTTCACGACCTATTGAAATCGAGTCTATAATGCATACTGGAGATTTTGAGGCATTTGAAGCAACCAGACAAGCTATGGAGGAGGTCATGTCCGCACTTAAAGATGATAACATCACTATCATTGGCATCAACGGCATGGGAGGTGTCGGCAAAACAACCATGGTTACATATGTCAGTTCACAAGCCAAAAGAGACGGGATCTTCGATCATGTGATTATGGCTTGTATATCTGAAAACCCTGACTTGATCAAAATTCAAGGCATATTAGCAGATATGTTGGGAATGCAATTGAAAGAGGAGACAGAAATTGGAAGAGCTGGTAGATTGAAGGATAGAATAATGAGAGGAAATAGGATTCTTATAATCTTCGACGACATGTGGAAGAATATAAATTTATCAAGCATAGGAATTCCCAGCTACAATGAGCTTAGAAAGTGTAATTCTAAACTCTTGCTCACCACAAGGAGATTATCTGTTTGTCATGTCATGAGGAGCCAAGCAATCATCCATCTTAACATCCTATCAAAAGAAGATTCTTGGAGCTTGTTTGTAAAGGAAGCGGGACATTCTTTTCATGAATCCACCAGATTTTATGATATAGCGAGGAAGGTAGCTAGAGAATGTGCTGGTCTCCCTATTGCATTGATAACAGTTGCAAGGGCACTGCGATATAAAGACATGGAAGAATGGGAAGAAGCAGCTCGGCGACTAGGGACGTCTCAACCTCCCAGCCTTGATGATGAGGGAGATGTTTTCAAATGTATAAAGTTGAGCTATGATTACTTGAAAAGTGATCTTGCCAAGTCATGCTTCTTGCTTTGCTGCCTATTCCCTGAAGACTATGACATCCGCATTGAAGACTTGCTTAAATATGGGATCAGTCATGGCTTATTTCGAGATTCCAACACAATGAATGAAGCCAGAGCCAGAATACATTCGGTGATCAAGTACCTTAAAGCATCAAACTTGCTTTTAGACAGTGAAATAGATGGATGTGTAAGGATGCATGATGTCATTCGGGATATGGCCATATCAACTACATTATCCAAGGACAACAATGGGTTTTTGGTGAAAGCTGGTTTACACAACCTTGCCGAAGAGTTGAAAAGGTCTCGAGTTGAGGAGCGATGGGAGCGTTTAGTCGGTGCAGTATTATGTTGGAAAGGAAAGGAATCAACTTCTGAAAATGGGAGCTATGGTACTGGCATTGATGCAAACGTTCAATCTCCATGGCTTGCTAATTGGGAGGTTCAGGAAATCCTGAAAGTTGCTGATGAAATACAACATAACGATCCCATTGCCAGTAGAATCTTATGCGAGCACAGTTATCAACTGGCACAGAATTTGAATTCTAATAGCGACGGAAGGGGAGTATTGCAACTCAAGAGTGGATTGATGTCTGTCTTGAAGGCAAAAGCAAGCACAGAGGGGGGTGAAACTGTGGATAGAAGCCAAGACTTTCTTGTCTTACAGAAGTTCTATAAACTTTATAGGGAGAAGAATAATGTAGAAAAGCTGCGGGAGGAAGAAATGAAGTTGAGGGAATCTGGATCTATCTGTGCAAATCTGGGGGAGTTGGAGAGAAAAACTAGTATAAGGAAAAGAGTTTCTGCAACTCTAAGAATTCTGGAGACAGTTTTGGAGCAGCTGGAGGAAACTCCTGAAGAGGAAACATCTCGAGTTGAGGAGTGTGGTACTTGCAATATTGGAAAGACATCTCAAGTTGAGGAGCGATGGGAGCATATAGTCCATGCAGTACTAAAACGGGAAAGGCTGGGAACTGATGCATACAGGCGGCATGGTACAGGCATTGTTGGAAAAGTTCGATCTTCATTTGCAATTAGCAGGGTTATAAATAAAATTTTGGAAGCTGCAGATGAAATCGAAGATGAAAATATTATTGCTTCTAGAATCTTATGTGAGCAGGGTTATTCGTTGGCTCAAGATCTGGATTGTGATAGTGAAGGAAACGGAGTATTACGGTTTAAGACTGGTCTGATGTGTTTCACTAAGCAAGGGCTATCAATAAGGAGAAGTGCAACCGCAACCATAGATAGAAGTGAAGACTTTGTTCTATTACAACAGTTCTATAAACTTTATAGGGAGAAGAATAGTGTCGAAAAGCTGCTGCAGGAAAAAATGAAGATGAGGGAGTGTAGTACTTCTTCCAGTGATAACCTTAGAGAGTAA

Protein Analysis

912

Amino Acids

103.89

Weight (kDa)

6.3

Isoelectric Point (pI)

55.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 17 - 182 9.1e-52 TIR domain
TIR_2 PF13676 20 - 137 5e-15 TIR domain
NB-ARC PF00931 207 - 372 1.4e-28 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 911, 2402
AasI GACNNNNNNGTC 1 cut(s) 2012
AatII GACGTC 1 cut(s) 1268
AciI CCGC 7 cut(s) 88, 642, 1109, 1399, 2068, 2344, 2592
AclI AACGTT 1 cut(s) 1767
AclWI GGATC 5 cut(s) 338, 755, 1433, 1835, 2107
AcoI YGGCCR 1 cut(s) 1575
AcsI RAATTY 8 cut(s) 318, 801, 940, 957, 1891, 1897, 2172, 2409
AcuI CTGAAG 2 cut(s) 1404, 2232
AcyI GRCGYC 1 cut(s) 1265
AfaI GTAC 8 cut(s) 357, 543, 1499, 1750, 2245, 2307, 2353, 2710
AfiI CCNNNNNNNGG 1 cut(s) 367
AflIII ACRYGT 1 cut(s) 924
AhlI ACTAGT 1 cut(s) 2138
AjuI GAANNNNNNNTTGG 4 cut(s) 238, 270, 1343, 1375
Alw21I GWGCWC 1 cut(s) 1872
Alw26I GTCTC 7 cut(s) 200, 735, 842, 1184, 1272, 1665, 2174
AlwI GGATC 5 cut(s) 338, 755, 1433, 1835, 2107
Ama87I CYCGRG 2 cut(s) 1662, 2226
AoxI GGCC 2 cut(s) 3, 1575
ApoI RAATTY 8 cut(s) 318, 801, 940, 957, 1891, 1897, 2172, 2409
ArsI GACNNNNNNTTYG 2 cut(s) 734, 766
Asp700I GAANNNNTTC 1 cut(s) 1483
AspS9I GGNCC 1 cut(s) 4
AsuHPI GGTGA 5 cut(s) 89, 997, 1501, 1630, 1994
AvaI CYCGRG 2 cut(s) 1662, 2226
BaeGI GKGCMC 1 cut(s) 1213
BalI TGGCCA 1 cut(s) 1577
BarI GAAGNNNNNNTAC 2 cut(s) 2698, 2730
BbsI GAAGAC 4 cut(s) 312, 1392, 1413, 2619
Bbv12I GWGCWC 1 cut(s) 1872
BbvCI CCTCAGC 1 cut(s) 19
BccI CCATC 6 cut(s) 236, 369, 1065, 1532, 1671, 2277
BceAI ACGGC 2 cut(s) 219, 697
BcgI CGANNNNNNTGC 2 cut(s) 1829, 1863
BciVI GTATCC 1 cut(s) 502
BclI TGATCA 3 cut(s) 157, 795, 1491
BcoDI GTCTC 7 cut(s) 200, 735, 842, 1184, 1272, 1665, 2174
BcuI ACTAGT 1 cut(s) 2138
BfaI CTAG 5 cut(s) 405, 1164, 1259, 2139, 2457
BfmI CTRYAG 2 cut(s) 2421, 2678
BfuI GTATCC 1 cut(s) 502
BglII AGATCT 1 cut(s) 2494
BmcAI AGTACT 3 cut(s) 543, 2307, 2710
BmeT110I CYCGRG 2 cut(s) 1662, 2226
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 1 cut(s) 1043
BmsI GCATC 7 cut(s) 430, 466, 684, 1517, 1542, 1750, 2323
BpiI GAAGAC 4 cut(s) 312, 1392, 1413, 2619
BpmI CTGGAG 3 cut(s) 609, 2198, 2219
Bpu10I CCTNAGC 1 cut(s) 19
BpuEI CTTGAG 3 cut(s) 1916, 2253, 2475
BsaHI GRCGYC 1 cut(s) 1265
BsaI GGTCTC 2 cut(s) 1184, 1665
BsaJI CCNNGG 3 cut(s) 708, 1596, 1778
BsaXI ACNNNNNCTCC 4 cut(s) 623, 653, 1732, 1762
Bsc4I CCNNNNNNNGG 1 cut(s) 367
Bse118I RCCGGY 1 cut(s) 28
Bse1I ACTGG 6 cut(s) 592, 1756, 1851, 1887, 2545, 2718
Bse3DI GCAATG 1 cut(s) 1845
BseDI CCNNGG 3 cut(s) 708, 1596, 1778
BseGI GGATG 8 cut(s) 160, 280, 481, 1053, 1065, 1395, 1547, 1557
BseLI CCNNNNNNNGG 1 cut(s) 367
BseMI GCAATG 1 cut(s) 1845
BseMII CTCAG 1 cut(s) 33
BseNI ACTGG 6 cut(s) 592, 1756, 1851, 1887, 2545, 2718
BseRI GAGGAG 7 cut(s) 27, 644, 860, 1054, 1685, 2249, 2291
BseSI GKGCMC 1 cut(s) 1213
BseYI CCCAGC 4 cut(s) 471, 962, 1277, 2323
BsgI GTGCAG 2 cut(s) 553, 1716
BshFI GGCC 2 cut(s) 5, 1577
BsiHKAI GWGCWC 1 cut(s) 1872
BsiHKCI CYCGRG 2 cut(s) 1662, 2226
BsiSI CCGG 1 cut(s) 29
BsiWI CGTACG 1 cut(s) 355
BslFI GGGAC 3 cut(s) 190, 1125, 1276
BslI CCNNNNNNNGG 1 cut(s) 367
BsmAI GTCTC 7 cut(s) 200, 735, 842, 1184, 1272, 1665, 2174
BsmBI CGTCTC 2 cut(s) 735, 1272
BsmFI GGGAC 3 cut(s) 190, 1125, 1276
BsmI GAATGC 1 cut(s) 837
BsnI GGCC 2 cut(s) 5, 1577
Bso31I GGTCTC 2 cut(s) 1184, 1665
BsoBI CYCGRG 2 cut(s) 1662, 2226
Bsp1286I GDGCHC 2 cut(s) 1213, 1872
Bsp19I CCATGG 2 cut(s) 708, 1778
BspACI CCGC 7 cut(s) 88, 642, 1109, 1399, 2068, 2344, 2592
BspANI GGCC 2 cut(s) 5, 1577
BspCNI CTCAG 1 cut(s) 32
BspHI TCATGA 2 cut(s) 1035, 1123
BspLI GGNNCC 1 cut(s) 1043
BspMAI CTGCAG 2 cut(s) 2425, 2682
BspPI GGATC 5 cut(s) 338, 755, 1433, 1835, 2107
BspQI GCTCTTC 1 cut(s) 856
BspTNI GGTCTC 2 cut(s) 1184, 1665
BsrDI GCAATG 1 cut(s) 1845
BsrFI RCCGGY 1 cut(s) 28
BsrI ACTGG 6 cut(s) 592, 1756, 1851, 1887, 2545, 2718
BssAI RCCGGY 1 cut(s) 28
BssECI CCNNGG 3 cut(s) 708, 1596, 1778
BssNI GRCGYC 1 cut(s) 1265
BssT1I CCWWGG 3 cut(s) 708, 1596, 1778
Bst4CI ACNGT 7 cut(s) 1201, 1529, 1874, 1990, 2185, 2532, 2634
Bst6I CTCTTC 3 cut(s) 856, 1641, 2208
BstACI GRCGYC 1 cut(s) 1265
BstC8I GCNNGC 3 cut(s) 1785, 1868, 1969
BstDEI CTNAG 6 cut(s) 11, 19, 977, 2168, 2559, 2729
BstDSI CCRYGG 2 cut(s) 708, 1778
BstF5I GGATG 8 cut(s) 160, 280, 481, 1053, 1065, 1395, 1547, 1557
BstMAI GTCTC 7 cut(s) 200, 735, 842, 1184, 1272, 1665, 2174
BstMWI GCNNNNNNNGC 5 cut(s) 29, 608, 681, 1681, 2287
BstNSI RCATGY 1 cut(s) 928
BstSFI CTRYAG 2 cut(s) 2421, 2678
BstSLI GKGCMC 1 cut(s) 1213
BstV2I GAAGAC 4 cut(s) 312, 1392, 1413, 2619
BstX2I RGATCY 3 cut(s) 747, 2099, 2494
BstYI RGATCY 3 cut(s) 747, 2099, 2494
BsuI GTATCC 1 cut(s) 502
BsuRI GGCC 2 cut(s) 5, 1577
BtgI CCRYGG 2 cut(s) 708, 1778
BtgZI GCGATG 2 cut(s) 1690, 2296
BtsCI GGATG 8 cut(s) 160, 280, 481, 1053, 1065, 1395, 1547, 1557
BtsI GCAGTG 2 cut(s) 437, 1211
BtsIMutI CAGTG 4 cut(s) 437, 1211, 1534, 2725
Cac8I GCNNGC 3 cut(s) 1785, 1868, 1969
CciI TCATGA 2 cut(s) 1035, 1123
Cfr10I RCCGGY 1 cut(s) 28
Cfr13I GGNCC 1 cut(s) 4
Csp6I GTAC 8 cut(s) 356, 542, 1498, 1749, 2244, 2306, 2352, 2709
CviQI GTAC 8 cut(s) 356, 542, 1498, 1749, 2244, 2306, 2352, 2709
DdeI CTNAG 6 cut(s) 11, 19, 977, 2168, 2559, 2729
DraI TTTAAA 1 cut(s) 447
DrdI GACNNNNNNGTC 1 cut(s) 2012
DseDI GACNNNNNNGTC 1 cut(s) 2012
EaeI YGGCCR 1 cut(s) 1575
Eam1104I CTCTTC 3 cut(s) 856, 1641, 2208
EarI CTCTTC 3 cut(s) 856, 1641, 2208
Eco130I CCWWGG 3 cut(s) 708, 1596, 1778
Eco31I GGTCTC 2 cut(s) 1184, 1665
Eco57I CTGAAG 2 cut(s) 1404, 2232
Eco88I CYCGRG 2 cut(s) 1662, 2226
EcoRI GAATTC 4 cut(s) 318, 957, 1897, 2172
EcoT14I CCWWGG 3 cut(s) 708, 1596, 1778
EcoT22I ATGCAT 4 cut(s) 302, 585, 1557, 2338
ErhI CCWWGG 3 cut(s) 708, 1596, 1778
Esp3I CGTCTC 2 cut(s) 735, 1272
FalI AAGNNNNNCTT 6 cut(s) 1343, 1375, 1398, 1430, 1995, 2027
FaqI GGGAC 3 cut(s) 190, 1125, 1276
FauI CCCGC 3 cut(s) 95, 1102, 2061
FauNDI CATATG 1 cut(s) 718
FbaI TGATCA 3 cut(s) 157, 795, 1491
FokI GGATG 8 cut(s) 167, 287, 488, 1040, 1052, 1382, 1554, 1564
FspBI CTAG 5 cut(s) 405, 1164, 1259, 2139, 2457
GsaI CCCAGC 4 cut(s) 475, 966, 1281, 2327
GsuI CTGGAG 3 cut(s) 609, 2198, 2219
HaeIII GGCC 2 cut(s) 5, 1577
HapII CCGG 1 cut(s) 29
Hin1I GRCGYC 1 cut(s) 1265
HpaII CCGG 1 cut(s) 29
HphI GGTGA 5 cut(s) 89, 997, 1501, 1630, 1994
Hpy166II GTNNAC 2 cut(s) 728, 1631
Hpy188I TCNGA 5 cut(s) 352, 496, 781, 1732, 2547
Hpy8I GTNNAC 2 cut(s) 728, 1631
Hpy99I CGWCG 3 cut(s) 45, 923, 1913
HpyAV CCTTC 5 cut(s) 871, 1150, 1908, 1951, 2507
HpyCH4III ACNGT 7 cut(s) 1201, 1529, 1874, 1990, 2185, 2532, 2634
HpyCH4IV ACGT 4 cut(s) 339, 354, 1265, 1767
HpyF10VI GCNNNNNNNGC 5 cut(s) 29, 608, 681, 1681, 2287
HpyF3I CTNAG 6 cut(s) 11, 19, 977, 2168, 2559, 2729
HpySE526I ACGT 4 cut(s) 339, 354, 1265, 1767
Hsp92I GRCGYC 1 cut(s) 1265
Ksp22I TGATCA 3 cut(s) 157, 795, 1491
LguI GCTCTTC 1 cut(s) 856
LmnI GCTCC 8 cut(s) 1041, 1089, 1672, 1681, 1740, 2191, 2278, 2287
LweI GCATC 7 cut(s) 430, 466, 684, 1517, 1542, 1750, 2323
MaeI CTAG 5 cut(s) 405, 1164, 1259, 2139, 2457
MaeII ACGT 4 cut(s) 339, 354, 1265, 1767
MaeIII GTNAC 2 cut(s) 520, 712
MfeI CAATTG 1 cut(s) 836
MflI RGATCY 3 cut(s) 747, 2099, 2494
MhlI GDGCHC 2 cut(s) 1213, 1872
MlsI TGGCCA 1 cut(s) 1577
MluNI TGGCCA 1 cut(s) 1577
MlyI GAGTC 1 cut(s) 581
MmeI TCCRAC 4 cut(s) 1476, 1688, 2106, 2344
Mox20I TGGCCA 1 cut(s) 1577
Mph1103I ATGCAT 4 cut(s) 302, 585, 1557, 2338
MroXI GAANNNNTTC 1 cut(s) 1483
MscI TGGCCA 1 cut(s) 1577
MseI TTAA 8 cut(s) 65, 141, 446, 648, 1062, 1416, 1503, 2535
MslI CAYNNNNRTG 1 cut(s) 529
Msp20I TGGCCA 1 cut(s) 1577
MspA1I CMGCKG 2 cut(s) 23, 2197
MspI CCGG 1 cut(s) 29
MunI CAATTG 1 cut(s) 836
Mva1269I GAATGC 1 cut(s) 837
MwoI GCNNNNNNNGC 5 cut(s) 29, 608, 681, 1681, 2287
NcoI CCATGG 2 cut(s) 708, 1778
NdeI CATATG 1 cut(s) 718
NlaIV GGNNCC 1 cut(s) 1043
NmeAIII GCCGAG 1 cut(s) 1231
NmuCI GTSAC 1 cut(s) 520
NsiI ATGCAT 4 cut(s) 302, 585, 1557, 2338
NspI RCATGY 1 cut(s) 928
PaeR7I CTCGAG 2 cut(s) 1662, 2226
PagI TCATGA 2 cut(s) 1035, 1123
PciI ACATGT 1 cut(s) 924
PciSI GCTCTTC 1 cut(s) 856
PcsI WCGNNNNNNNCGW 1 cut(s) 750
PctI GAATGC 1 cut(s) 837
PdmI GAANNNNTTC 1 cut(s) 1483
Pfl23II CGTACG 1 cut(s) 355
PflFI GACNNNGTC 1 cut(s) 422
PleI GAGTC 1 cut(s) 580
PpsI GAGTC 1 cut(s) 580
PscI ACATGT 1 cut(s) 924
PsiI TTATAA 2 cut(s) 911, 2402
Psp1406I AACGTT 1 cut(s) 1767
PspFI CCCAGC 4 cut(s) 471, 962, 1277, 2323
PspLI CGTACG 1 cut(s) 355
PspN4I GGNNCC 1 cut(s) 1043
PspPI GGNCC 1 cut(s) 4
PstI CTGCAG 2 cut(s) 2425, 2682
PsuI RGATCY 3 cut(s) 747, 2099, 2494
PsyI GACNNNGTC 1 cut(s) 422
PvuII CAGCTG 2 cut(s) 23, 2197
RsaI GTAC 8 cut(s) 357, 543, 1499, 1750, 2245, 2307, 2353, 2710
RsaNI GTAC 8 cut(s) 356, 542, 1498, 1749, 2244, 2306, 2352, 2709
RseI CAYNNNNRTG 1 cut(s) 529
SapI GCTCTTC 1 cut(s) 856
SaqAI TTAA 8 cut(s) 65, 141, 446, 648, 1062, 1416, 1503, 2535
Sau96I GGNCC 1 cut(s) 4
ScaI AGTACT 3 cut(s) 543, 2307, 2710
SchI GAGTC 1 cut(s) 581
SduI GDGCHC 2 cut(s) 1213, 1872
SfaNI GCATC 7 cut(s) 430, 466, 684, 1517, 1542, 1750, 2323
SfcI CTRYAG 2 cut(s) 2421, 2678
Sfr274I CTCGAG 2 cut(s) 1662, 2226
SlaI CTCGAG 2 cut(s) 1662, 2226
SmiMI CAYNNNNRTG 1 cut(s) 529
SmlI CTYRAG 5 cut(s) 1662, 1931, 2226, 2268, 2490
SmoI CTYRAG 5 cut(s) 1662, 1931, 2226, 2268, 2490
SpeI ACTAGT 1 cut(s) 2138
SsiI CCGC 7 cut(s) 88, 642, 1109, 1399, 2068, 2344, 2592
SspI AATATT 2 cut(s) 2254, 2446
SspMI CTAG 5 cut(s) 405, 1164, 1259, 2139, 2457
StyI CCWWGG 3 cut(s) 708, 1596, 1778
TaaI ACNGT 7 cut(s) 1201, 1529, 1874, 1990, 2185, 2532, 2634
TaiI ACGT 4 cut(s) 342, 357, 1268, 1770
TaqI TCGA 9 cut(s) 570, 753, 918, 1444, 1663, 2227, 2374, 2433, 2667
TatI WGTACW 3 cut(s) 541, 2305, 2708
TauI GCSGC 1 cut(s) 2347
Tru1I TTAA 8 cut(s) 65, 141, 446, 648, 1062, 1416, 1503, 2535
Tru9I TTAA 8 cut(s) 65, 141, 446, 648, 1062, 1416, 1503, 2535
TscAI CASTG 4 cut(s) 437, 1218, 1534, 2725
TseFI GTSAC 1 cut(s) 520
Tsp45I GTSAC 1 cut(s) 520
TspGWI ACGGA 2 cut(s) 1926, 2535
TspRI CASTG 4 cut(s) 437, 1218, 1534, 2725
Tth111I GACNNNGTC 1 cut(s) 422
XapI RAATTY 8 cut(s) 318, 801, 940, 957, 1891, 1897, 2172, 2409
XbaI TCTAGA 1 cut(s) 2456
XceI RCATGY 1 cut(s) 928
XcmI CCANNNNNNNNNTGG 1 cut(s) 622
XhoI CTCGAG 2 cut(s) 1662, 2226
XmnI GAANNNNTTC 1 cut(s) 1483
XspI CTAG 5 cut(s) 405, 1164, 1259, 2139, 2457
ZraI GACGTC 1 cut(s) 1266
ZrmI AGTACT 3 cut(s) 543, 2307, 2710
Zsp2I ATGCAT 4 cut(s) 302, 585, 1557, 2338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.