RchiOBHm_Chr5g0061301

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000085
Physical Location & Seq
Reverse (-)
66703994 .. 66710642
6649 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33768

Sequence Viewer

Length: 4530 bp
ATGGCCTTGGAATCCCCAACGGCCTCTGCATCACTTTCTACTTCAGTTGAATCATCGGCTCCTGGGTGGAAGTATGAGGTGTTTTTGAGTTTCAGGAGTGAAGACGGTTTTACTTCTTATTTGTACAATGAATTGCAAAAGAGAGAAATCAAGACATTCATGGATATCGACGGAACTAGTTTGGATAGAGGAGAGGGGATTTCTCAAAGTATCCTAGAGGCCATTCAAGAATCAAGATTTGCAATCGTTGTTCTCTCGCAAAATTATGCTTCTTCCAGTTGGTGTTTGGATGAACTCACACAGATTATGAAGTGCATGGAAAATAAGGAAACAATTCTGCCAGTTTTCTATCACGTGGATCCCTCTGAAGTGCGCTCTCAAAGGGGGGTGTGCGGAGCGGCCTTGGCTGCACATGAAGAGCGGTTTAAAGATCAAAAAGAGAGGCTGATGAAATGGAGGGACGCCTTGAACAATATCAGCAGCATCTTTGGCTGGGTATGTGGGAGTTCTGAGCCAGAAGGACAGATTGTCCAACAAATCGCGGAATTTGTCTCCAGCAAACCTATTGAAATTAAGTCGACGGAAGATATTGAAACTCATGGAACTTCTCCTCGTTGGAAGTATGATGTGTTTATAAATTTCAGGGGTGAGGACACTTGTAAGGTTTTTCTGTCCCATTTACTAGCTGAATTGCAAAAGAAACCAGCACACCTGAACACAGTAAAGAATGATGAACCACTTCCAAAAGGGCGGAATATATCTTCGTATCTCCTAAACGCCATAGAAGAGGTGAGGTTTGCCATTGTTGTTCTTTCGCAAGACTTTGCTTATTCATCATGGTGCCTAGAGGAACTAGCAAAGATTTTTGAATGCATGGAAAACCAAAATAGAATTCTGCCAATCTTTTATCATGTGGATCCCTCTGAGGTGCAACATCAAAGTGGGAGATTTGGAGATGCCTTCGCTGTACATGAAGAAAGATTTCGGGATAATCATGAGAAGGTCAGGCGGTGGAGACGTGTTTTATCAATGGTAGCCAACATCCCTGGATGGACTTCAGAAAACTATGAGTATGAAGGAGAACTTATCAAAGACGTTGTGCAAGCTATGTGCAGTAGACTAGTTCAGTCTACAGAGTCGACAGAAGATTTTGAAGCAACAGGAGATGTCATGGACCGAACTTCTACCCAGCCTGACCCAGCTCCTGACCCAGCCCATTCAGACCATGAATTGCCAATCATGTCTCCGCCTTCATCAGCTGAACGAGATCCTCGGCCGAAGTATGATGTGTTTTTGAGTTTCAGGGGGGAGGACACTCGTAAGGGTTTTATAGCCCATTTATACCGTGAATTGCAAAACACCAGAGTGATTAAAACTTTCAAGGATGACGTGCAACTAGAAAAAGGGACGGTCATTGCTTCAAATCTCCTAACGGCAATCGAAGAATCAAGATTTGCGATCATTGTGTTGTCAAAAAATTATGCTTCTTCTTCATGGTGTTTGGATGAACTTGCAAAGATTTTCCAATGCATGAAAGATAGGAACACAATTTTCCCAATTTTTTATGAGGTGGAACCCACTGATGTACGCCATCAAAAGACAGAGACGAGGTTTGGAAAAGCTTTCATTAAGCAGCACGAAACTCATGGACAAGACAACGAGAAGATGAAGCAATGGAGTGCTGCTTTAGAAGGAGTGGCTGGAATCGCTGGGTTCCCGTCAACTAAGTATCAGACGGACGTAGAACTAATCAAAGACGTTGTGAAACATTTGTTGAGCAAAGTACTTCAAGCACCCACCGGAAATTTTGAAACATTTAAAGCGACAAGGCGAGCAATGGATGACGTCATGGAGGTATTAAAAGATGATCAAGTCACTGTCCTTGGGGTGCATGGAGTGGGTGGCGTTGGAAAGACGACGATGGTCAAACATGTTAGTGCACAAGCCCAGAAAGATGGGATTTTTGATCATGTGATTATGGTTGTCGTATCCAAAAGCCCTGACTTGCAAGAAATTCAAGGCACATTGGCAGGTTCGCTGGGCTTGGAGGAGGGGATCATGAGAGAAAATAGGATGCTTATAATATTGGACGACATTTGGGAGAGTTTTGAACTCTCGAGCATTGGAATTCCCAGTCACAATGAGCTTCAAAGGTGCAATTCTAAAGTTATACTCACCACAAGGAAAAGCAATGTCTGCGATTCTATGGGGTGCCACGCCCAGATCCTTCTCAATGTTCTGTCAGAGGAAGACTCTTGGAAGTTATTCATGGAGACGTCGGGGAAGTCCTTTCATGAATCTACCATTTCCTATGATGAAGCGAGAAAGGTAGCTCGAGAATGCTGTGGTCTCCCAAAAGCAATAAAAGCAGCTGCAAGGTCACTTGGAGATAAATACATAGACGAATGGAATGAAGCAGTTGTAACAGCATTTGGAGATGACTGCTTGGACAAATGGAATGAAACAGCTCCACCTGTCAACTTCGAAGCTGAGGAAGACGCATTTCATTCATTGGCATCTGATGATGCCAAGTCATGCTTCTTGCTATGCTGCCTATTCCCCGAAAATTATGATATCCCAATTGAAGATCTGCTCAAGTATGGGATTGGAAAAGGTTTGTTTCAATATGATACAATGCAAGAAGCCAGAGATAGAGCACATTCTGTGGTCAAGTACCTCAAAGTTTCTAGATTGCTTTTAGACGGTAGAAAGAAGGGATGCGTAAGGATGACTGATGGCATTCGAAATCTGGCCATGTCCATTGCATTGTCCAAAGAAGGCGATGGGTTTTTAGTAAAAGCTGGTTGTGAACTAGAGGAGTGGCCAGAGGCTGGATGGCTGCAAGAAATCACTTCCAGGAACTGGAATGAAACCCATGAAGCCTACTCAACAATCTCACTCATGAGAAACAAGCTTCACAAGCTTCCCGAATATTTGGTATGTTCAAAACTCCAAATTTTATTGCTACAAAGTAATGCCGGAATATGTGACATCCCAGAAACCTTTTTGCAAAGTCCTAATCCGCTAAGGGTCTTTGATCTTAGCTTCACTGGTGTTTCGTCACTACCTTCATCATTCAGTTTCCTAACCAACCTCCAAACTTTGAATTTGGATTCTTGCAAAAATTTAAGTAACATATCCGTTCTAGGAAAACTTAATAAGAAACTTAAGATTCTTAGTATGAGAGAACTTCCTCTTAAGAAATTTCCAAAACAAATAGGAGATTTGACCAGTCTAAGGATGTTGGATGTCACTGGTAGATATCTTGAAACTATCCCATCTAAAGTGATATCAAGGTTGTATAGATTGGAAGAACTGCACATGAAATTTCAGTTTGGGAAGTGGGGAAGTAAAATTGAGGATGCAGTTGATGAAGAAACCAATGCTGACTTTGATGAGTTGACTGGCTTGTTGGATTTAAACATTTTGGAAGTTTACATATCTGATGCCGCATGCTTGCCTAAAAGTGTTGAGTACATGCCGAATTGGGCAGAATTTGATATATGCATCAGCAGACACGTCCCTTGCGTGGCGCTCCCTCAACATGATGGTTTTTCAAGAACCTTGACTCTTGACACAACAATCAGAAGCTTACCGTATTGGTTTATCAGCGCTGTGACAGAGAAGGTGGAGAAGCTAGAGTACACAGATTGCGAAGACTTGAATGACATCGTTAGGGAATATGACCATGGCAGATTACTTGCACTGAAATGTCTCTCTGTGAACGGGAACCGCAAGAACTTGAAAGAGATAATCAACACAATGACACGGGTTTCAAATAAACCAGTTTTTGAGAACTTGGAAGAGTTGCATCTGCACCGCTTATATTCCCTCAAGGAGTTATGTGTTGGTGAGCTACCACCTGGGTCTCTACGCAAGCTCAAGTTCTTGAAAGTGCAAACTTGTCGTAGCTTGGTGAATGCACTTTTACCATCAACATTGTTGCAGAGACTACAAAATCTGGAAGAAATCATTTGTGTAAATCTGGAAGGAATGAAATATGTATTTGGATCTGATGAAGGGCTTGATCCAGAAAATATTTTCTTGAGGAAATTGAGGGAGATGAGATTGTCTGCTCTATACGAACTAGTAAGCATATGTAATGGTCCTGCTCCGCGTGTAATCTTCCAGAATCTTAAAGTTTTGGCAATTTGCGAGTGCAAGGAATTGAAAAATCTCTTCACAATAGATGTAGTTGAATGTCTTTGTCAGTTGGAAGACCTTTCCATAGAGCACTGTCCTAGCTTGGACAGAGTCATTGAAGCAAGCAATAGCTTGAACAGCAAGATTAAACTTCTGAAGTTGAAGAAGTTAGCTTTGATAGATCTTCCGGTGCTCACAAGGTTCTGCACTGAAGGTGGTACTATTGAAACTGAGTGCCCTTCATTGGAATACTTGTATGTGGAGAAGTGCCTCCAGTTTTCGTATTTTGCTTACCACTTAGACAACAAGAACCAAGTCTACTTCAATGATAAACAACATTTCAGTTCTTTAGTGAAAAGGTGGGAGGAAGTGCATAATCGGCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1509

Amino Acids

171.56

Weight (kDa)

5.41

Isoelectric Point (pI)

46.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 24 - 193 5.7e-44 TIR domain
TIR_2 PF13676 27 - 121 3.8e-15 TIR domain
TIR PF01582 207 - 385 5.8e-46 TIR domain
TIR_2 PF13676 210 - 307 5.8e-09 TIR domain
TIR PF01582 427 - 601 2.1e-47 TIR domain
TIR_2 PF13676 430 - 526 1.4e-13 TIR domain
NB-ARC PF00931 612 - 760 1.8e-26 NB-ARC domain
LRR_14 PF23598 981 - 1117 6.5e-09 Leucine-rich repeat region
LRR_RPS2 PF23247 1267 - 1413 3.5e-24 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1399 - 1474 4.1e-06 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 635, 2081
AatII GACGTC 2 cut(s) 1848, 2279
Acc36I ACCTGC 1 cut(s) 2021
AccB1I GGYRCC 2 cut(s) 840, 2211
AccB7I CCANNNNNTGG 2 cut(s) 2831, 2862
AccBSI CCGCTC 2 cut(s) 398, 421
AccI GTMKAC 5 cut(s) 578, 1117, 1130, 1139, 4461
AccII CGCG 2 cut(s) 542, 4117
AclWI GGATC 9 cut(s) 353, 366, 911, 924, 1262, 2063, 2218, 4018, 4022
AcoI YGGCCR 3 cut(s) 1274, 2751, 2822
AcuI CTGAAG 5 cut(s) 27, 387, 1041, 4319, 4374
AcvI CACGTG 1 cut(s) 355
AcyI GRCGYC 3 cut(s) 462, 1845, 2276
AfaI GTAC 8 cut(s) 125, 969, 1587, 1785, 2675, 3476, 3644, 4363
AfeI AGCGCT 1 cut(s) 3613
AfiI CCNNNNNNNGG 5 cut(s) 2831, 2862, 3237, 3529, 4387
AflII CTTAAG 2 cut(s) 3167, 3197
AflIII ACRYGT 3 cut(s) 1018, 1930, 3517
AhdI GACNNNNNGTC 1 cut(s) 527
AhlI ACTAGT 3 cut(s) 176, 1120, 4087
AjiI CACGTC 3 cut(s) 1019, 1390, 3520
AjnI CCWGG 4 cut(s) 61, 1045, 2855, 3862
AjuI GAANNNNNNNTTGG 4 cut(s) 876, 908, 2522, 2554
AleI CACNNNNGTG 1 cut(s) 1364
Alw21I GWGCWC 4 cut(s) 1942, 2659, 4236, 4338
Alw26I GTCTC 9 cut(s) 556, 1009, 1248, 1598, 2267, 2354, 3719, 3873, 3943
Alw44I GTGCAC 1 cut(s) 1938
AlwI GGATC 9 cut(s) 353, 366, 911, 924, 1262, 2063, 2218, 4018, 4022
AlwNI CAGNNNCTG 3 cut(s) 1205, 2831, 2862
Ama87I CYCGRG 2 cut(s) 2116, 2334
Aor51HI AGCGCT 1 cut(s) 3613
AoxI GGCC 7 cut(s) 3, 21, 219, 399, 1274, 2751, 2822
ApaLI GTGCAC 1 cut(s) 1938
ApeKI GCWGC 8 cut(s) 407, 480, 1633, 1682, 2369, 2372, 2550, 2839
Asp700I GAANNNNTTC 4 cut(s) 333, 738, 981, 2264
AspLEI GCGC 3 cut(s) 375, 3535, 3614
AspS9I GGNCC 2 cut(s) 1174, 4106
AsuHPI GGTGA 5 cut(s) 659, 802, 2167, 3863, 3928
AsuII TTCGAA 2 cut(s) 2484, 2743
AvaI CYCGRG 2 cut(s) 2116, 2334
AvaII GGWCC 2 cut(s) 1174, 4106
BaeGI GKGCMC 2 cut(s) 1942, 4382
BalI TGGCCA 2 cut(s) 2753, 2824
BamHI GGATCC 2 cut(s) 358, 916
BanI GGYRCC 2 cut(s) 840, 2211
BarI GAAGNNNNNNTAC 2 cut(s) 3626, 3658
BbrPI CACGTG 1 cut(s) 355
BbsI GAAGAC 5 cut(s) 108, 2256, 2502, 3663, 4224
Bbv12I GWGCWC 4 cut(s) 1942, 2659, 4236, 4338
BbvCI CCTCAGC 1 cut(s) 2490
BbvI GCAGC 8 cut(s) 394, 492, 1645, 1669, 2359, 2381, 2537, 2826
BceAI ACGGC 2 cut(s) 36, 1449
BcgI CGANNNNNNTGC 2 cut(s) 3887, 3921
BciT130I CCWGG 4 cut(s) 63, 1047, 2857, 3864
BciVI GTATCC 2 cut(s) 221, 1999
BclI TGATCA 2 cut(s) 1867, 1966
BcoDI GTCTC 9 cut(s) 556, 1009, 1248, 1598, 2267, 2354, 3719, 3873, 3943
BcuI ACTAGT 3 cut(s) 176, 1120, 4087
BfmI CTRYAG 1 cut(s) 1131
BfoI RGCGCY 2 cut(s) 3536, 3615
BfrI CTTAAG 2 cut(s) 3167, 3197
BfuAI ACCTGC 1 cut(s) 2021
BfuI GTATCC 2 cut(s) 221, 1999
BglII AGATCT 2 cut(s) 2587, 4324
BmcAI AGTACT 1 cut(s) 1785
Bme1390I CCNGG 4 cut(s) 63, 1047, 2857, 3864
Bme18I GGWCC 2 cut(s) 1174, 4106
BmeRI GACNNNNNGTC 1 cut(s) 527
BmeT110I CYCGRG 2 cut(s) 2116, 2334
BmgBI CACGTC 3 cut(s) 1019, 1390, 3520
BmgT120I GGNCC 2 cut(s) 1174, 4106
BmiI GGNNCC 8 cut(s) 60, 360, 842, 918, 1575, 1715, 2213, 3731
BmrFI CCNGG 4 cut(s) 63, 1047, 2857, 3864
BmrI ACTGGG 1 cut(s) 2127
BmuI ACTGGG 1 cut(s) 2127
BoxI GACNNNNGTC 1 cut(s) 1922
BpiI GAAGAC 5 cut(s) 108, 2256, 2502, 3663, 4224
BplI GAGNNNNNCTC 2 cut(s) 2237, 2269
BpmI CTGGAG 2 cut(s) 538, 4400
Bpu10I CCTNAGC 2 cut(s) 2490, 3026
Bpu14I TTCGAA 2 cut(s) 2484, 2743
BpuEI CTTGAG 4 cut(s) 2579, 3818, 3866, 4066
BsaAI YACGTR 1 cut(s) 355
BsaHI GRCGYC 3 cut(s) 462, 1845, 2276
BsaI GGTCTC 2 cut(s) 2354, 3873
BsaJI CCNNGG 8 cut(s) 6, 62, 402, 1045, 1271, 1882, 3688, 3863
BsaWI WCCGGW 2 cut(s) 1799, 4330
Bsc4I CCNNNNNNNGG 5 cut(s) 2831, 2862, 3237, 3529, 4387
Bse3DI GCAATG 5 cut(s) 1413, 1679, 1842, 2197, 2760
BseBI CCWGG 4 cut(s) 63, 1047, 2857, 3864
BseDI CCNNGG 8 cut(s) 6, 62, 402, 1045, 1271, 1882, 3688, 3863
BseLI CCNNNNNNNGG 5 cut(s) 2831, 2862, 3237, 3529, 4387
BseMI GCAATG 5 cut(s) 1413, 1679, 1842, 2197, 2760
BseMII CTCAG 4 cut(s) 501, 915, 2481, 4365
BseRI GAGGAG 4 cut(s) 204, 600, 2063, 2831
BseSI GKGCMC 2 cut(s) 1942, 4382
BseX3I CGGCCG 1 cut(s) 1274
BseXI GCAGC 8 cut(s) 394, 492, 1645, 1669, 2359, 2381, 2537, 2826
BseYI CCCAGC 6 cut(s) 492, 1188, 1198, 1210, 1709, 2038
BsgI GTGCAG 5 cut(s) 393, 1132, 3302, 3800, 4333
Bsh1236I CGCG 2 cut(s) 542, 4117
Bsh1285I CGRYCG 1 cut(s) 1277
BshFI GGCC 7 cut(s) 5, 23, 221, 401, 1276, 2753, 2824
BshNI GGYRCC 2 cut(s) 840, 2211
BsiEI CGRYCG 1 cut(s) 1277
BsiHKAI GWGCWC 4 cut(s) 1942, 2659, 4236, 4338
BsiHKCI CYCGRG 2 cut(s) 2116, 2334
BsiSI CCGG 3 cut(s) 1800, 2979, 4331
BslFI GGGAC 4 cut(s) 473, 658, 1420, 3506
BslI CCNNNNNNNGG 5 cut(s) 2831, 2862, 3237, 3529, 4387
BsmAI GTCTC 9 cut(s) 556, 1009, 1248, 1598, 2267, 2354, 3719, 3873, 3943
BsmBI CGTCTC 3 cut(s) 1009, 1598, 2267
BsmFI GGGAC 4 cut(s) 473, 658, 1420, 3506
BsmI GAATGC 4 cut(s) 875, 2345, 2739, 3925
BsnI GGCC 7 cut(s) 5, 23, 221, 401, 1276, 2753, 2824
Bso31I GGTCTC 2 cut(s) 2354, 3873
BsoBI CYCGRG 2 cut(s) 2116, 2334
Bsp119I TTCGAA 2 cut(s) 2484, 2743
Bsp1286I GDGCHC 5 cut(s) 1942, 2659, 4236, 4338, 4382
Bsp1407I TGTACA 2 cut(s) 123, 967
Bsp19I CCATGG 1 cut(s) 3688
BspANI GGCC 7 cut(s) 5, 23, 221, 401, 1276, 2753, 2824
BspCNI CTCAG 4 cut(s) 502, 916, 2482, 4366
BspFNI CGCG 2 cut(s) 542, 4117
BspHI TCATGA 4 cut(s) 994, 2058, 2293, 2901
BspLI GGNNCC 8 cut(s) 60, 360, 842, 918, 1575, 1715, 2213, 3731
BspMI ACCTGC 1 cut(s) 2021
BspPI GGATC 9 cut(s) 353, 366, 911, 924, 1262, 2063, 2218, 4018, 4022
BspQI GCTCTTC 1 cut(s) 411
BspT104I TTCGAA 2 cut(s) 2484, 2743
BspT107I GGYRCC 2 cut(s) 840, 2211
BspTI CTTAAG 2 cut(s) 3167, 3197
BspTNI GGTCTC 2 cut(s) 2354, 3873
BsrBI CCGCTC 2 cut(s) 398, 421
BsrDI GCAATG 5 cut(s) 1413, 1679, 1842, 2197, 2760
BsrGI TGTACA 2 cut(s) 123, 967
BssECI CCNNGG 8 cut(s) 6, 62, 402, 1045, 1271, 1882, 3688, 3863
BssNI GRCGYC 3 cut(s) 462, 1845, 2276
BssT1I CCWWGG 4 cut(s) 6, 402, 1882, 3688
Bst2UI CCWGG 4 cut(s) 63, 1047, 2857, 3864
Bst4CI ACNGT 8 cut(s) 107, 721, 1346, 1411, 1879, 2705, 3597, 4238
Bst6I CTCTTC 4 cut(s) 411, 780, 3798, 4184
BstACI GRCGYC 3 cut(s) 462, 1845, 2276
BstAFI CTTAAG 2 cut(s) 3167, 3197
BstAPI GCANNNNNTGC 1 cut(s) 2196
BstAUI TGTACA 2 cut(s) 123, 967
BstBAI YACGTR 1 cut(s) 355
BstBI TTCGAA 2 cut(s) 2484, 2743
BstC8I GCNNGC 6 cut(s) 1104, 1833, 3454, 3458, 3878, 4267
BstDSI CCRYGG 1 cut(s) 3688
BstFNI CGCG 2 cut(s) 542, 4117
BstH2I RGCGCY 2 cut(s) 3536, 3615
BstHHI GCGC 3 cut(s) 375, 3535, 3614
BstMAI GTCTC 9 cut(s) 556, 1009, 1248, 1598, 2267, 2354, 3719, 3873, 3943
BstMCI CGRYCG 1 cut(s) 1277
BstMWI GCNNNNNNNGC 9 cut(s) 404, 407, 489, 1706, 2196, 2366, 2920, 4281, 4522
BstNI CCWGG 4 cut(s) 63, 1047, 2857, 3864
BstNSI RCATGY 3 cut(s) 1934, 3456, 3481
BstPAI GACNNNNGTC 1 cut(s) 1922
BstSCI CCNGG 4 cut(s) 61, 1045, 2855, 3862
BstSFI CTRYAG 1 cut(s) 1131
BstSLI GKGCMC 2 cut(s) 1942, 4382
BstUI CGCG 2 cut(s) 542, 4117
BstV1I GCAGC 8 cut(s) 394, 492, 1645, 1669, 2359, 2381, 2537, 2826
BstV2I GAAGAC 5 cut(s) 108, 2256, 2502, 3663, 4224
BstX2I RGATCY 7 cut(s) 358, 916, 1267, 2223, 2587, 4010, 4324
BstXI CCANNNNNNTGG 1 cut(s) 1955
BstYI RGATCY 7 cut(s) 358, 916, 1267, 2223, 2587, 4010, 4324
BstZI CGGCCG 1 cut(s) 1274
BsuI GTATCC 2 cut(s) 221, 1999
BsuRI GGCC 7 cut(s) 5, 23, 221, 401, 1276, 2753, 2824
BtgI CCRYGG 1 cut(s) 3688
BtgZI GCGATG 1 cut(s) 2796
BtrI CACGTC 3 cut(s) 1019, 1390, 3520
BtsIMutI CAGTG 7 cut(s) 1578, 1875, 3048, 3252, 3704, 4234, 4350
BveI ACCTGC 1 cut(s) 2021
Cac8I GCNNGC 6 cut(s) 1104, 1833, 3454, 3458, 3878, 4267
CaiI CAGNNNCTG 3 cut(s) 1205, 2831, 2862
CciI TCATGA 4 cut(s) 994, 2058, 2293, 2901
CfoI GCGC 3 cut(s) 375, 3535, 3614
Cfr13I GGNCC 2 cut(s) 1174, 4106
CseI GACGC 2 cut(s) 470, 2507
Csp6I GTAC 8 cut(s) 124, 968, 1586, 1784, 2674, 3475, 3643, 4362
CviQI GTAC 8 cut(s) 124, 968, 1586, 1784, 2674, 3475, 3643, 4362
DraI TTTAAA 3 cut(s) 427, 1819, 3420
DriI GACNNNNNGTC 1 cut(s) 527
EaeI YGGCCR 3 cut(s) 1274, 2751, 2822
EagI CGGCCG 1 cut(s) 1274
Eam1104I CTCTTC 4 cut(s) 411, 780, 3798, 4184
Eam1105I GACNNNNNGTC 1 cut(s) 527
EarI CTCTTC 4 cut(s) 411, 780, 3798, 4184
EciI GGCGGA 2 cut(s) 766, 1236
EclXI CGGCCG 1 cut(s) 1274
Eco130I CCWWGG 4 cut(s) 6, 402, 1882, 3688
Eco31I GGTCTC 2 cut(s) 2354, 3873
Eco32I GATATC 4 cut(s) 166, 2575, 3263, 3291
Eco47I GGWCC 2 cut(s) 1174, 4106
Eco47III AGCGCT 1 cut(s) 3613
Eco52I CGGCCG 1 cut(s) 1274
Eco57I CTGAAG 5 cut(s) 27, 387, 1041, 4319, 4374
Eco72I CACGTG 1 cut(s) 355
Eco88I CYCGRG 2 cut(s) 2116, 2334
EcoRI GAATTC 2 cut(s) 891, 2127
EcoRII CCWGG 4 cut(s) 61, 1045, 2855, 3862
EcoRV GATATC 4 cut(s) 166, 2575, 3263, 3291
EcoT14I CCWWGG 4 cut(s) 6, 402, 1882, 3688
EcoT22I ATGCAT 3 cut(s) 875, 1532, 3509
ErhI CCWWGG 4 cut(s) 6, 402, 1882, 3688
Esp3I CGTCTC 3 cut(s) 1009, 1598, 2267
FalI AAGNNNNNCTT 4 cut(s) 1068, 1100, 2522, 2554
FaqI GGGAC 4 cut(s) 473, 658, 1420, 3506
FauNDI CATATG 1 cut(s) 4097
FbaI TGATCA 2 cut(s) 1867, 1966
FblI GTMKAC 5 cut(s) 578, 1117, 1130, 1139, 4461
GlaI GCGC 3 cut(s) 374, 3534, 3613
GsaI CCCAGC 6 cut(s) 496, 1192, 1202, 1214, 1713, 2042
GsuI CTGGAG 2 cut(s) 538, 4400
HaeII RGCGCY 2 cut(s) 3536, 3615
HaeIII GGCC 7 cut(s) 5, 23, 221, 401, 1276, 2753, 2824
HapII CCGG 3 cut(s) 1800, 2979, 4331
HgaI GACGC 2 cut(s) 470, 2507
HhaI GCGC 3 cut(s) 375, 3535, 3614
Hin1I GRCGYC 3 cut(s) 462, 1845, 2276
Hin6I GCGC 3 cut(s) 373, 3533, 3612
HinP1I GCGC 3 cut(s) 373, 3533, 3612
HincII GTYRAC 5 cut(s) 579, 1140, 1722, 2479, 3402
HindII GTYRAC 5 cut(s) 579, 1140, 1722, 2479, 3402
HindIII AAGCTT 4 cut(s) 1620, 2912, 2921, 3589
HpaII CCGG 3 cut(s) 1800, 2979, 4331
HphI GGTGA 5 cut(s) 659, 802, 2167, 3863, 3928
Hpy99I CGWCG 4 cut(s) 173, 583, 1921, 2281
HpyCH4III ACNGT 8 cut(s) 107, 721, 1346, 1411, 1879, 2705, 3597, 4238
HpyCH4IV ACGT 9 cut(s) 354, 1018, 1095, 1389, 1740, 1758, 1845, 2276, 3519
HpyF10VI GCNNNNNNNGC 9 cut(s) 404, 407, 489, 1706, 2196, 2366, 2920, 4281, 4522
HpySE526I ACGT 9 cut(s) 354, 1018, 1095, 1389, 1740, 1758, 1845, 2276, 3519
Hsp92I GRCGYC 3 cut(s) 462, 1845, 2276
HspAI GCGC 3 cut(s) 373, 3533, 3612
Ksp22I TGATCA 2 cut(s) 1867, 1966
LguI GCTCTTC 1 cut(s) 411
LmnI GCTCC 6 cut(s) 64, 395, 1207, 2473, 3540, 4117
Lsp1109I GCAGC 8 cut(s) 394, 492, 1645, 1669, 2359, 2381, 2537, 2826
MaeII ACGT 9 cut(s) 354, 1018, 1095, 1389, 1740, 1758, 1845, 2276, 3519
MaeIII GTNAC 9 cut(s) 1873, 2135, 2379, 2422, 2987, 3060, 3131, 3250, 3616
MbiI CCGCTC 2 cut(s) 398, 421
MfeI CAATTG 1 cut(s) 2580
MflI RGATCY 7 cut(s) 358, 916, 1267, 2223, 2587, 4010, 4324
MhlI GDGCHC 5 cut(s) 1942, 2659, 4236, 4338, 4382
MlsI TGGCCA 2 cut(s) 2753, 2824
MluNI TGGCCA 2 cut(s) 2753, 2824
MlyI GAGTC 4 cut(s) 1145, 2246, 3562, 4263
MmeI TCCRAC 6 cut(s) 556, 596, 1888, 3225, 3393, 4194
Mox20I TGGCCA 2 cut(s) 2753, 2824
Mph1103I ATGCAT 3 cut(s) 875, 1532, 3509
MroXI GAANNNNTTC 4 cut(s) 333, 738, 981, 2264
MscI TGGCCA 2 cut(s) 2753, 2824
MslI CAYNNNNRTG 5 cut(s) 838, 939, 1364, 1935, 3709
Msp20I TGGCCA 2 cut(s) 2753, 2824
MspA1I CMGCKG 2 cut(s) 1259, 2372
MspCI CTTAAG 2 cut(s) 3167, 3197
MspI CCGG 3 cut(s) 1800, 2979, 4331
MspR9I CCNGG 4 cut(s) 63, 1047, 2857, 3864
MunI CAATTG 1 cut(s) 2580
Mva1269I GAATGC 4 cut(s) 875, 2345, 2739, 3925
MvaI CCWGG 4 cut(s) 63, 1047, 2857, 3864
MvnI CGCG 2 cut(s) 542, 4117
MwoI GCNNNNNNNGC 9 cut(s) 404, 407, 489, 1706, 2196, 2366, 2920, 4281, 4522
NcoI CCATGG 1 cut(s) 3688
NdeI CATATG 1 cut(s) 4097
NlaIV GGNNCC 8 cut(s) 60, 360, 842, 918, 1575, 1715, 2213, 3731
NmeAIII GCCGAG 1 cut(s) 1252
NmuCI GTSAC 7 cut(s) 1873, 2135, 2379, 2987, 3060, 3250, 3616
NsiI ATGCAT 3 cut(s) 875, 1532, 3509
NspI RCATGY 3 cut(s) 1934, 3456, 3481
NspV TTCGAA 2 cut(s) 2484, 2743
OliI CACNNNNGTG 1 cut(s) 1364
PaeI GCATGC 1 cut(s) 3456
PaeR7I CTCGAG 2 cut(s) 2116, 2334
PagI TCATGA 4 cut(s) 994, 2058, 2293, 2901
PciI ACATGT 1 cut(s) 1930
PciSI GCTCTTC 1 cut(s) 411
PcsI WCGNNNNNNNCGW 1 cut(s) 3525
PctI GAATGC 4 cut(s) 875, 2345, 2739, 3925
PdmI GAANNNNTTC 4 cut(s) 333, 738, 981, 2264
PflFI GACNNNGTC 1 cut(s) 4253
PflMI CCANNNNNTGG 2 cut(s) 2831, 2862
PfoI TCCNGGA 1 cut(s) 2855
PleI GAGTC 4 cut(s) 1144, 2246, 3562, 4262
PmaCI CACGTG 1 cut(s) 355
PmlI CACGTG 1 cut(s) 355
PpsI GAGTC 4 cut(s) 1144, 2246, 3562, 4262
Ppu21I YACGTR 1 cut(s) 355
PscI ACATGT 1 cut(s) 1930
PshAI GACNNNNGTC 1 cut(s) 1922
PsiI TTATAA 2 cut(s) 635, 2081
Psp6I CCWGG 4 cut(s) 61, 1045, 2855, 3862
PspCI CACGTG 1 cut(s) 355
PspFI CCCAGC 6 cut(s) 492, 1188, 1198, 1210, 1709, 2038
PspGI CCWGG 4 cut(s) 61, 1045, 2855, 3862
PspN4I GGNNCC 8 cut(s) 60, 360, 842, 918, 1575, 1715, 2213, 3731
PspPI GGNCC 2 cut(s) 1174, 4106
PsrI GAACNNNNNNTAC 2 cut(s) 4445, 4477
PstNI CAGNNNCTG 3 cut(s) 1205, 2831, 2862
PsuI RGATCY 7 cut(s) 358, 916, 1267, 2223, 2587, 4010, 4324
PsyI GACNNNGTC 1 cut(s) 4253
PvuII CAGCTG 2 cut(s) 1259, 2372
RsaI GTAC 8 cut(s) 125, 969, 1587, 1785, 2675, 3476, 3644, 4363
RsaNI GTAC 8 cut(s) 124, 968, 1586, 1784, 2674, 3475, 3643, 4362
RseI CAYNNNNRTG 5 cut(s) 838, 939, 1364, 1935, 3709
SalI GTCGAC 2 cut(s) 577, 1138
SapI GCTCTTC 1 cut(s) 411
Sau96I GGNCC 2 cut(s) 1174, 4106
ScaI AGTACT 1 cut(s) 1785
SchI GAGTC 4 cut(s) 1145, 2246, 3562, 4263
ScrFI CCNGG 4 cut(s) 63, 1047, 2857, 3864
SduI GDGCHC 5 cut(s) 1942, 2659, 4236, 4338, 4382
SfcI CTRYAG 1 cut(s) 1131
Sfr274I CTCGAG 2 cut(s) 2116, 2334
SfuI TTCGAA 2 cut(s) 2484, 2743
SinI GGWCC 2 cut(s) 1174, 4106
SlaI CTCGAG 2 cut(s) 2116, 2334
SmiMI CAYNNNNRTG 5 cut(s) 838, 939, 1364, 1935, 3709
SmlI CTYRAG 8 cut(s) 2116, 2334, 2594, 3167, 3197, 3833, 3881, 4045
SmoI CTYRAG 8 cut(s) 2116, 2334, 2594, 3167, 3197, 3833, 3881, 4045
SpeI ACTAGT 3 cut(s) 176, 1120, 4087
SphI GCATGC 1 cut(s) 3456
SspI AATATT 3 cut(s) 2085, 2933, 4039
StyD4I CCNGG 4 cut(s) 61, 1045, 2855, 3862
StyI CCWWGG 4 cut(s) 6, 402, 1882, 3688
TaaI ACNGT 8 cut(s) 107, 721, 1346, 1411, 1879, 2705, 3597, 4238
TaiI ACGT 9 cut(s) 357, 1021, 1098, 1392, 1743, 1761, 1848, 2279, 3522
TaqI TCGA 8 cut(s) 168, 578, 1139, 1440, 2117, 2335, 2484, 2743
TaqII GACCGA 1 cut(s) 1191
TatI WGTACW 5 cut(s) 123, 967, 1783, 3474, 3642
TauI GCSGC 2 cut(s) 401, 3452
TscAI CASTG 7 cut(s) 1585, 1882, 3055, 3259, 3711, 4241, 4357
TseFI GTSAC 7 cut(s) 1873, 2135, 2379, 2987, 3060, 3250, 3616
TseI GCWGC 8 cut(s) 407, 480, 1633, 1682, 2369, 2372, 2550, 2839
Tsp45I GTSAC 7 cut(s) 1873, 2135, 2379, 2987, 3060, 3250, 3616
TspGWI ACGGA 4 cut(s) 186, 596, 1751, 3130
TspRI CASTG 7 cut(s) 1585, 1882, 3055, 3259, 3711, 4241, 4357
Tth111I GACNNNGTC 1 cut(s) 4253
Van91I CCANNNNNTGG 2 cut(s) 2831, 2862
Vha464I CTTAAG 2 cut(s) 3167, 3197
VneI GTGCAC 1 cut(s) 1938
VpaK11BI GGWCC 2 cut(s) 1174, 4106
XbaI TCTAGA 1 cut(s) 2687
XceI RCATGY 3 cut(s) 1934, 3456, 3481
XcmI CCANNNNNNNNNTGG 2 cut(s) 283, 2832
XhoI CTCGAG 2 cut(s) 2116, 2334
XmiI GTMKAC 5 cut(s) 578, 1117, 1130, 1139, 4461
XmnI GAANNNNTTC 4 cut(s) 333, 738, 981, 2264
ZraI GACGTC 2 cut(s) 1846, 2277
ZrmI AGTACT 1 cut(s) 1785
Zsp2I ATGCAT 3 cut(s) 875, 1532, 3509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.