FvH4_5g02970

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000085
Physical Location & Seq
Reverse (-)
1780976 .. 1788852
7877 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g02970.t1

Sequence Viewer

Length: 5058 bp
ATGGCCTTGAACACCCCAACTGCCTCTGCATCGCTTCCTACATCAGCTGAATCATCGGCGGCTCCTCGGTGGAAGTATGACGTGTTTTTGAGTTTCCGGCATGAAGACACTGGCCGGGGTTTTACTTCTTATTTGTACAATGAATTGCAAAAGAGAGAAATCAAGACATTCATGGATAACGACGAAATGATTTTGGATAGAGGTGAGGGGATCTCTCAAGTTATCCTAGAGGCAATTCAAGAATCAAGATTTGCAATCGTTGTTCTGTCGGAAAACTATGCTTCTTCCAGTTGGTGTTTGGATGAACTCACACAGATCATTCAGTGCATGGATTTTAAGGAAACAGTAGTTCTGCCAGTTTTCTATCATTTGGATCCCTCTGATGTGCGTCATCAAAGGGGGGCGTATGGACATGCCTTCGTTGAACATGAACAGAGGTTTGGAGATCAAACAGAGAGGCTGAGGAAGTGGAAAGACGCATTGCTTACAGTGAGCGACATCTTTGGCTGGTCAAAAAATGATTATGAGTCAGAAAGACAATTTGTCAAACAAATCGTGGAATTTATCTGCAGTAGACCTATTGAAAGTGAAACAAGACAAGCAACAGATGAAATTTCTCCTCGATGGAAGCATGATGTGTTTATAAATTTCAGGGGTATGGACACTCGTAACGGTTTTCTGTCCCACTTACACGATGAATTGCAAAATACAGAAAACCTGAACATAGCAAGGGATGACGGACCAGATGCAAAAGGGCAGCTTATAGTTCCGTATCTTCTAAAGGCAATTGAAGAGTCAAGGTTTGCCCTCGTTGTTCTCTCACCAAATTTTGCTGGTTCTTCATGGTGCTTGGAAGAACTTGCGAAGATTTTTGAATGCATGGGAGGCCAGAAAAGAATTCTGCCAATCTTTTATAAAGTGGATCCCTCTGATGTGCGACGCCAAACTGGGAGTTTTGGAGAAGGCTTGGCTCGCTTTGAAGACAGATTTGAAAGAGACAAGGTCTTGAAGTGGAGACGTGTTTTAACAATGGTGGCCAATCTCTCTGGCTTCGATTCAAGTAATTATGGGTATGAAGGAGAACTTATCAGACACGTTGCGGAAGAGTGCAGTAGACGAGTTACAGAGTTGACAGGAGATTTTGAAACAACAGGAGATGTTCGGTCCATGGCTTCTCCCCAGCCTGAGCCAGCTCCTGTGAAGAATGATGTGCTTTCAGGTTTCGGGATAGAAGACACTCGCAGGGGTTTTGTAGCCCATTCAGACCATGAATTGCAAAACACAGGAGTAAATAGAACATTCAGGGAACACGAAACACAAAACACCGATGAAAATCATGTTGAAGAAGAGATAGTTATTCCCTCTACGTCTCCACCTTCACCTGCTGTGGGAGATCCTGAAAACGGGGTAGCCCTCCCATCTACATCACCAGTTATTGGGCAACAAAATGATGAGTTTTTGAATACCAGGGAAGAGGACAATCATAGGGGTTTTGTCACCCATTTGGGTCACGAAACACAAAACACCGATGCAAATCATGTTGAAGATGAGATAGCTGTCCCCTCTACGTCTCCACCTTCACTAGCTGTGGGAGATCCTGAAAACGGGGTAGCCCTCCCATCTACTTCACCAGTTATTGGGCAGCAAAATGATGAGTTTTTGAATACCAGGGGAGAGGACAATCATAGGGGTTTTGTCACCCATTTGGGTCACGAAACACAAAACACCGATGAAAATCATCTTGAAGAAGAGATAGCTGTCCCCTCTACGTCTCCATCTTCACCAGCTGTGGGAGATCCTCGGGTGAAGTATGATGTGTTTTTGAGTTTCAGGGGGGAAGATACTCGAAAGGGTTTTATAGCCTATTTATACCGTGAATTGCAAAAAACCAGAGCGATTAAAACTTTCAAGGATGATGTGCAACTGAAAAAAGGGACTGTTATTGCTTCAAATCTCCTAAGGGCAATCGAAGAATCAAGGTTTGCGATTATTGTGTTTTCCGAGAAGTATGCTGATTCTGCATGGTGTTTGGATGAACTTACAAAGATTTTTGAATGCATGGAGGGTAAGGACACAATTCTCCCAATTTTTTATAAGTTGGATCCCGCTGTCGTACGATATCAAAAGAAAGAGACCAGTTTTGGTGAAGCTTTCGTCAAGCATGAAGCTAGACATGACATTGAGAAGACGAACCAGTGGAGAGCTGCATTAAAAGGAGTGGCAGGAATCTCTGGGTGGGATTTAAACCGTTTTCCGACGGAGTTAGAACTAATCGATGACATTGTGGAACATTTGTTGGAAAAAGTACTTCAAGCACCCAACAGAACTTTTGGAACATTTGAAGCGACAAGGCAAGCCATGGATGAGGTCATGAAGGTGTTAAAAGATGATCAAGTCACTGTCCTTGGGGTGCATGGGGTAGCTGGCGTTGGAAAGACGACTATGGTCAAACATGTTGGTGCACAAGCTAAGAAAGATGGGCTTTTTGATCGTGTGATTATGGTTGTCATATCCAAAAGCCCTGACTTGCAAGAAATTCAAGGCACGCTAGCAGATTCATTGGGCTTGAAGGAGGGGATCAAGAGAGGAGATAGGATGCTTATAATCTTGGACGACATTTGGGAGAGTTTTGAACTCTTAGGCATTGGAATTCCCGGTCACCATGAGCTTCAAAACTGTTACTCTAAAGTTGTAATCATCACAAGGAACAGCAACGTCTGCCATTCTATGGGGTGTCATGCCCAGATCGCTCTCAATATTCTGTCAGATGAAGATTCTTGGAAGTTACTCAAAGAGACCTCTGGGAAGTCTTTTGATGAATCTGCCAAGTCCTATGATGAAGAGAGGAAGGTAGCTCAAGAATGCAGGGGTCTCCCAAGAGCAATAAAAGCAGCTGCAAGGTCACTTGGAGATAAATACTGGGACAAATGGAATGAAGCAGTTGCAAGGGAATTCGGAGGCTTGGACGAATGGAATGAAGAAGCCCAAGCCGTCAACCTTGAAGCTGAGGAGGGCGCTTCTCATTCATTGGAGGATACATATCACTCACTGGAATCTGATGATGCCAAGTCATGCTTCTTGCTATGCTGCTTGTTCCCAGAAAATTATGATATCCCGATTGAGGAGTTGCTCAAGTATGGGATTGGAATAGGTTTGTTTCAATATGATTCAATGCAAGATGCCAGAGATAGAGCACATTATGTGGTTAAGTACCTCAAAGATTCTAGATTACTTCTGGAGGGTACAAAGGAGGGATGTGTAAGGATGACCGATGACATTCGAAATCTGGCCATGTCCATTGCATTGTCTGAGGAAGGCGATGGGCTTTTAGTAAAAGCTGGTTGTGAGCTAGAGGAGTGGCCAGACGCAGAGGCAGGATGGTGGCAAGGATGGACTTGGACCCATTCCTGGCTCTGGGGCTACTCAATAATCTCACTAATGAGAAACAAGCTTCACAAGCTTCCCAAATATTTGGTATGTTCAAAGCTCCAAATTTTGTTACTACAAAGTAATGCTGGTATATGTGACATTCCAGAAACCTTTCTTCAAAGTCCTAATGCGCTAAGGGTCTTTGATCTTAGCTCCACCAGTATTTCGTCACTACCTCAGTCATTCAATGTCCTAACCAGTCTCCAAGCTTTGAATTTGGATTTTTGCAGCAATTTAAGTGAGATATCTGTCTTAGAAAATCTTAAGAAACTTGAGATTCTTAGTATGAGAGAACTTCCTCTTAAGAATTTACCAAAACAAATAGGAAATTTGACCAATCTGAGGAAGTTGGACGTCACTGGTAGATCTCTTGAAACAATCCCATCTGAAGTGTTATCGAAGTTGTGTAGATTGGAAGAACTGCACTTGAAGTTTCAATTTGGGAAGTGGGGAAGTAAAATTGAGGATGCATCTGAAGAGACCAACGCTAACTTTGATGAGTTGACTGGTTTGCCTGATTTAAACATTTTGGAAGTTTACATATCTGATGCTACATGCTTGCCTCAAAGTGTTGAGTACTTCAAGCCGAATTGGGTAGAATTTGATATATGCATCAGCAGGGACGTGTTGCTCCCTCAATATGATGGTTTTTCAAGAACCTTGACTCTTGACACAACAATCAATAGCTTACCAGATTGGTTTATCAGCATTGTGACAGCGAAAGTGGAGCAGCTAGAGTACACAAATTGCGATGATTTGAATGACCTTGTTGGGGAATATGACCATGGCAGATTATTTGTGCTGAAATATCTCTCTGTGAACGGGCACCATAAGAACTTGAAAGAGATAATCAACACAATGAGACGGGTTTCAAATAAACCGGTATTTGAGAACTTGGAAGAGTTGCATCTGCACTGCTTATATTCCCTCGAGGAGTTGTGTGTTGGTGAGCTACCACCTGGTTCCCTACGCAAGCTCAAGTTATTGAAAGTGCAAACATGTCATAGCTTGGTGAATGCACTTTTATCATTGACATTATTGCCGAGATTACAAAATCTGGAAAAAATCATTTGTGTACATCTAGACAGAATGAAATATGTTTTTGGATCTGATGAAGTGGGTGTTGATCCAGAGAAGATTTTCTTGAGGAAATTGAGGGAGATGAGATTGTGTGCTCTAGAAGAACTAGTAAGAATATGTAATGGTCCTGCTTCGCATGCAATCTTCCAGAATCTAAAAGTTTTGGCAATCTGCAAATGCAAGGAACTGAAAAGCCTCTTCACAATAGACGTTGCTGAATGTCTTTTTCAGTTGGAAGACCTTTGCATAGAGCAGTGTCCTCGCTTGGACAGAGTCATTGAAGCAAGCAATAGCTTGAACAGCGAGATTACACTTCCGGCGTTGAAGAGATTAGCTTTGATTGATCTTCCGGTGCTCACAAGGTTCTGCACTGAAGGTGGTAATATTGAAACTCGGTGCCCTTCATTGGAATACTTGTCCGTGAAGAATTGCATCAAGTTTTCATATTTTGCAAACTCCCACTTCAGTAAGAAGGAAATTCACTTCAATGATCTAGACCATTTGAGTTCTCTAGAGGAAAGGTGGAAGGAAATGCATAATCAGCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1686

Amino Acids

191.03

Weight (kDa)

5.11

Isoelectric Point (pI)

44.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 25 - 196 7.6e-49 TIR domain
TIR_2 PF13676 28 - 126 1.4e-14 TIR domain
TIR PF01582 210 - 378 1.8e-43 TIR domain
TIR_2 PF13676 213 - 314 8.6e-09 TIR domain
TIR PF01582 603 - 775 6.5e-46 TIR domain
TIR_2 PF13676 606 - 702 1.8e-12 TIR domain
NB-ARC PF00931 785 - 935 9.1e-23 NB-ARC domain
LRR_14 PF23598 1146 - 1329 1.8e-11 Leucine-rich repeat region
LRR_8 PF13855 1162 - 1219 4.6e-07 Leucine rich repeat
LRR_RPS2 PF23247 1442 - 1589 1.1e-21 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 644, 915, 2094, 2603
AarI CACCTGC 1 cut(s) 1390
AatII GACGTC 1 cut(s) 3786
AbsI CCTCGAGG 1 cut(s) 4358
Acc36I ACCTGC 1 cut(s) 1390
AccB1I GGYRCC 2 cut(s) 4254, 4905
AccB7I CCANNNNNTGG 3 cut(s) 1436, 1637, 2728
AccI GTMKAC 2 cut(s) 574, 1114
AciI CCGC 3 cut(s) 59, 1100, 2106
AcoI YGGCCR 4 cut(s) 112, 1035, 3288, 3359
AcuI CTGAAG 4 cut(s) 3837, 3924, 4902, 4957
AcyI GRCGYC 2 cut(s) 940, 3783
AfaI GTAC 8 cut(s) 137, 2115, 2307, 3212, 3244, 4007, 4169, 4506
AflII CTTAAG 2 cut(s) 3692, 3731
AflIII ACRYGT 6 cut(s) 81, 1018, 1093, 2452, 4053, 4427
AgeI ACCGGT 1 cut(s) 4309
AhdI GACNNNNNGTC 1 cut(s) 542
AhlI ACTAGT 1 cut(s) 4615
AjiI CACGTC 3 cut(s) 82, 1019, 4054
AjnI CCWGG 4 cut(s) 1466, 1667, 3407, 4387
AjuI GAANNNNNNNTTGG 4 cut(s) 423, 455, 3059, 3091
Alw21I GWGCWC 4 cut(s) 2464, 3196, 4606, 4866
Alw44I GTGCAC 1 cut(s) 2460
AlwNI CAGNNNCTG 1 cut(s) 1196
Ama87I CYCGRG 2 cut(s) 1800, 4358
AoxI GGCC 6 cut(s) 3, 112, 886, 1035, 3288, 3359
ApaLI GTGCAC 1 cut(s) 2460
ApeKI GCWGC 8 cut(s) 757, 1642, 2204, 2891, 2894, 3087, 3657, 4159
AsiGI ACCGGT 1 cut(s) 4309
Asp700I GAANNNNTTC 2 cut(s) 3540, 4568
AspLEI GCGC 2 cut(s) 3017, 3562
AspS9I GGNCC 4 cut(s) 740, 1164, 3399, 4634
AsuC2I CCSGG 2 cut(s) 116, 2655
AsuII TTCGAA 1 cut(s) 3280
AsuNHI GCTAGC 1 cut(s) 2548
AvaI CYCGRG 2 cut(s) 1800, 4358
AvaII GGWCC 4 cut(s) 740, 1164, 3399, 4634
AxyI CCTNAGG 1 cut(s) 1958
BaeGI GKGCMC 3 cut(s) 2464, 4257, 4910
BalI TGGCCA 3 cut(s) 1037, 3290, 3361
BamHI GGATCC 3 cut(s) 373, 922, 2101
BanI GGYRCC 2 cut(s) 4254, 4905
BbsI GAAGAC 5 cut(s) 111, 987, 1239, 2192, 4752
Bbv12I GWGCWC 4 cut(s) 2464, 3196, 4606, 4866
BbvCI CCTCAGC 2 cut(s) 461, 3006
BbvI GCAGC 8 cut(s) 769, 1654, 2191, 2881, 2903, 3074, 3669, 4171
BceAI ACGGC 1 cut(s) 2975
BcgI CGANNNNNNTGC 4 cut(s) 1991, 2025, 4751, 4785
BciT130I CCWGG 4 cut(s) 1468, 1669, 3409, 4389
BciVI GTATCC 1 cut(s) 3028
BclI TGATCA 1 cut(s) 2389
BcnI CCSGG 2 cut(s) 116, 2655
BcuI ACTAGT 1 cut(s) 4615
BfmI CTRYAG 1 cut(s) 568
BfoI RGCGCY 1 cut(s) 3018
BfrI CTTAAG 2 cut(s) 3692, 3731
BfuAI ACCTGC 1 cut(s) 1390
BfuI GTATCC 1 cut(s) 3028
BglII AGATCT 1 cut(s) 3794
BisI GCNGC 9 cut(s) 60, 758, 1643, 2205, 2892, 2895, 3088, 3658, 4160
BlsI GCNGC 9 cut(s) 61, 759, 1644, 2206, 2893, 2896, 3089, 3659, 4161
BmcAI AGTACT 2 cut(s) 2307, 4007
Bme1390I CCNGG 6 cut(s) 116, 1468, 1669, 2655, 3409, 4389
Bme18I GGWCC 4 cut(s) 740, 1164, 3399, 4634
BmeRI GACNNNNNGTC 1 cut(s) 542
BmeT110I CYCGRG 2 cut(s) 1800, 4358
BmgBI CACGTC 3 cut(s) 82, 1019, 4054
BmgT120I GGNCC 4 cut(s) 740, 1164, 3399, 4634
BmiI GGNNCC 8 cut(s) 63, 375, 924, 2103, 3401, 4256, 4393, 4907
BmrFI CCNGG 6 cut(s) 116, 1468, 1669, 2655, 3409, 4389
BmrI ACTGGG 2 cut(s) 957, 2929
BmtI GCTAGC 1 cut(s) 2552
BmuI ACTGGG 2 cut(s) 957, 2929
BoxI GACNNNNGTC 1 cut(s) 2444
BpiI GAAGAC 5 cut(s) 111, 987, 1239, 2192, 4752
BplI GAGNNNNNCTC 2 cut(s) 197, 229
BpmI CTGGAG 1 cut(s) 3257
Bpu10I CCTNAGC 4 cut(s) 461, 1185, 3006, 3563
Bpu14I TTCGAA 1 cut(s) 3280
BpuEI CTTGAG 6 cut(s) 201, 2841, 3116, 3722, 4391, 4594
BpuMI CCSGG 2 cut(s) 116, 2655
Bsa29I ATCGAT 1 cut(s) 2274
BsaBI GATNNNNATC 4 cut(s) 1332, 1533, 1734, 3039
BsaHI GRCGYC 2 cut(s) 940, 3783
BsaI GGTCTC 4 cut(s) 2126, 2789, 2876, 3902
BsaJI CCNNGG 9 cut(s) 65, 115, 1167, 1467, 1668, 1799, 2358, 2404, 4213
BsaWI WCCGGW 2 cut(s) 4309, 4858
Bse118I RCCGGY 1 cut(s) 4309
Bse21I CCTNAGG 1 cut(s) 1958
Bse3DI GCAATG 2 cut(s) 479, 3297
Bse8I GATNNNNATC 4 cut(s) 1332, 1533, 1734, 3039
BseBI CCWGG 4 cut(s) 1468, 1669, 3409, 4389
BseCI ATCGAT 1 cut(s) 2274
BseDI CCNNGG 9 cut(s) 65, 115, 1167, 1467, 1668, 1799, 2358, 2404, 4213
BseJI GATNNNNATC 4 cut(s) 1332, 1533, 1734, 3039
BseMI GCAATG 2 cut(s) 479, 3297
BseMII CTCAG 6 cut(s) 452, 1176, 2997, 3300, 3620, 3761
BseRI GAGGAG 7 cut(s) 54, 609, 2601, 3023, 3137, 3368, 4376
BseSI GKGCMC 3 cut(s) 2464, 4257, 4910
BseXI GCAGC 8 cut(s) 769, 1654, 2191, 2881, 2903, 3074, 3669, 4171
BseYI CCCAGC 1 cut(s) 1179
BsgI GTGCAG 4 cut(s) 1129, 3836, 4325, 4861
BshFI GGCC 6 cut(s) 5, 114, 888, 1037, 3290, 3361
BshNI GGYRCC 2 cut(s) 4254, 4905
BshTI ACCGGT 1 cut(s) 4309
BshVI ATCGAT 1 cut(s) 2274
BsiHKAI GWGCWC 4 cut(s) 2464, 3196, 4606, 4866
BsiHKCI CYCGRG 2 cut(s) 1800, 4358
BsiSI CCGG 6 cut(s) 97, 115, 2655, 4310, 4826, 4859
BsiWI CGTACG 1 cut(s) 2113
BslFI GGGAC 6 cut(s) 667, 1544, 1745, 1948, 2936, 4064
BsmBI CGTCTC 5 cut(s) 1009, 1374, 1575, 1776, 4285
BsmFI GGGAC 6 cut(s) 667, 1544, 1745, 1948, 2936, 4064
BsmI GAATGC 4 cut(s) 881, 2060, 2867, 4450
BsnI GGCC 6 cut(s) 5, 114, 888, 1037, 3290, 3361
Bso31I GGTCTC 4 cut(s) 2126, 2789, 2876, 3902
BsoBI CYCGRG 2 cut(s) 1800, 4358
Bsp119I TTCGAA 1 cut(s) 3280
Bsp1286I GDGCHC 6 cut(s) 2464, 3196, 4257, 4606, 4866, 4910
Bsp1407I TGTACA 2 cut(s) 135, 4504
Bsp19I CCATGG 3 cut(s) 1167, 2358, 4213
BspACI CCGC 3 cut(s) 59, 1100, 2106
BspANI GGCC 6 cut(s) 5, 114, 888, 1037, 3290, 3361
BspCNI CTCAG 6 cut(s) 453, 1177, 2998, 3301, 3619, 3762
BspDI ATCGAT 1 cut(s) 2274
BspHI TCATGA 1 cut(s) 2370
BspLI GGNNCC 8 cut(s) 63, 375, 924, 2103, 3401, 4256, 4393, 4907
BspMAI CTGCAG 1 cut(s) 572
BspMI ACCTGC 1 cut(s) 1390
BspOI GCTAGC 1 cut(s) 2552
BspT104I TTCGAA 1 cut(s) 3280
BspT107I GGYRCC 2 cut(s) 4254, 4905
BspTI CTTAAG 2 cut(s) 3692, 3731
BspTNI GGTCTC 4 cut(s) 2126, 2789, 2876, 3902
BsrDI GCAATG 2 cut(s) 479, 3297
BsrFI RCCGGY 1 cut(s) 4309
BsrGI TGTACA 2 cut(s) 135, 4504
BssAI RCCGGY 1 cut(s) 4309
BssECI CCNNGG 9 cut(s) 65, 115, 1167, 1467, 1668, 1799, 2358, 2404, 4213
BssNI GRCGYC 2 cut(s) 940, 3783
BssT1I CCWWGG 4 cut(s) 1167, 2358, 2404, 4213
Bst2UI CCWGG 4 cut(s) 1468, 1669, 3409, 4389
Bst4CI ACNGT 8 cut(s) 346, 490, 674, 1874, 1939, 2249, 2401, 2678
BstACI GRCGYC 2 cut(s) 940, 3783
BstAFI CTTAAG 2 cut(s) 3692, 3731
BstAPI GCANNNNNTGC 1 cut(s) 2718
BstAUI TGTACA 2 cut(s) 135, 4504
BstBI TTCGAA 1 cut(s) 3280
BstDSI CCRYGG 3 cut(s) 1167, 2358, 4213
BstEII GGTNACC 1 cut(s) 2657
BstH2I RGCGCY 1 cut(s) 3018
BstHHI GCGC 2 cut(s) 3017, 3562
BstNI CCWGG 4 cut(s) 1468, 1669, 3409, 4389
BstNSI RCATGY 5 cut(s) 416, 2456, 3987, 4431, 4649
BstPAI GACNNNNGTC 1 cut(s) 2444
BstPI GGTNACC 1 cut(s) 2657
BstSCI CCNGG 6 cut(s) 114, 1466, 1667, 2653, 3407, 4387
BstSFI CTRYAG 1 cut(s) 568
BstSLI GKGCMC 3 cut(s) 2464, 4257, 4910
BstV1I GCAGC 8 cut(s) 769, 1654, 2191, 2881, 2903, 3074, 3669, 4171
BstV2I GAAGAC 5 cut(s) 111, 987, 1239, 2192, 4752
BstX2I RGATCY 9 cut(s) 210, 373, 922, 1393, 1594, 1795, 2101, 3794, 4535
BstXI CCANNNNNNTGG 1 cut(s) 3472
BstYI RGATCY 9 cut(s) 210, 373, 922, 1393, 1594, 1795, 2101, 3794, 4535
Bsu15I ATCGAT 1 cut(s) 2274
Bsu36I CCTNAGG 1 cut(s) 1958
BsuI GTATCC 1 cut(s) 3028
BsuRI GGCC 6 cut(s) 5, 114, 888, 1037, 3290, 3361
BsuTUI ATCGAT 1 cut(s) 2274
BtgI CCRYGG 3 cut(s) 1167, 2358, 4213
BtgZI GCGATG 3 cut(s) 15, 3333, 4194
BtrI CACGTC 3 cut(s) 82, 1019, 4054
BtsI GCAGTG 2 cut(s) 4342, 4769
BveI ACCTGC 1 cut(s) 1390
CaiI CAGNNNCTG 1 cut(s) 1196
CciI TCATGA 1 cut(s) 2370
CfoI GCGC 2 cut(s) 3017, 3562
Cfr10I RCCGGY 1 cut(s) 4309
Cfr13I GGNCC 4 cut(s) 740, 1164, 3399, 4634
ClaI ATCGAT 1 cut(s) 2274
CseI GACGC 4 cut(s) 377, 485, 948, 3374
CsiI ACCWGGT 1 cut(s) 4387
Csp6I GTAC 8 cut(s) 136, 2114, 2306, 3211, 3243, 4006, 4168, 4505
CspAI ACCGGT 1 cut(s) 4309
CviQI GTAC 8 cut(s) 136, 2114, 2306, 3211, 3243, 4006, 4168, 4505
DraI TTTAAA 2 cut(s) 2244, 3951
DriI GACNNNNNGTC 1 cut(s) 542
EaeI YGGCCR 4 cut(s) 112, 1035, 3288, 3359
Eam1105I GACNNNNNGTC 1 cut(s) 542
Eco130I CCWWGG 4 cut(s) 1167, 2358, 2404, 4213
Eco31I GGTCTC 4 cut(s) 2126, 2789, 2876, 3902
Eco32I GATATC 3 cut(s) 2120, 3112, 3675
Eco47I GGWCC 4 cut(s) 740, 1164, 3399, 4634
Eco57I CTGAAG 4 cut(s) 3837, 3924, 4902, 4957
Eco81I CCTNAGG 1 cut(s) 1958
Eco88I CYCGRG 2 cut(s) 1800, 4358
Eco91I GGTNACC 1 cut(s) 2657
EcoO65I GGTNACC 1 cut(s) 2657
EcoRI GAATTC 3 cut(s) 897, 2649, 2951
EcoRII CCWGG 4 cut(s) 1466, 1667, 3407, 4387
EcoRV GATATC 3 cut(s) 2120, 3112, 3675
EcoT14I CCWWGG 4 cut(s) 1167, 2358, 2404, 4213
EcoT22I ATGCAT 5 cut(s) 881, 2060, 3901, 4043, 5046
ErhI CCWWGG 4 cut(s) 1167, 2358, 2404, 4213
Esp3I CGTCTC 5 cut(s) 1009, 1374, 1575, 1776, 4285
FaqI GGGAC 6 cut(s) 667, 1544, 1745, 1948, 2936, 4064
FauI CCCGC 1 cut(s) 2113
FbaI TGATCA 1 cut(s) 2389
FblI GTMKAC 2 cut(s) 574, 1114
Fnu4HI GCNGC 9 cut(s) 60, 758, 1643, 2205, 2892, 2895, 3088, 3658, 4160
Fsp4HI GCNGC 9 cut(s) 60, 758, 1643, 2205, 2892, 2895, 3088, 3658, 4160
GlaI GCGC 2 cut(s) 3016, 3561
GluI GCNGC 9 cut(s) 60, 758, 1643, 2205, 2892, 2895, 3088, 3658, 4160
GsaI CCCAGC 1 cut(s) 1183
GsuI CTGGAG 1 cut(s) 3257
HaeII RGCGCY 1 cut(s) 3018
HaeIII GGCC 6 cut(s) 5, 114, 888, 1037, 3290, 3361
HapII CCGG 6 cut(s) 97, 115, 2655, 4310, 4826, 4859
HgaI GACGC 4 cut(s) 377, 485, 948, 3374
HhaI GCGC 2 cut(s) 3017, 3562
Hin1I GRCGYC 2 cut(s) 940, 3783
Hin6I GCGC 2 cut(s) 3015, 3560
HinP1I GCGC 2 cut(s) 3015, 3560
HincII GTYRAC 3 cut(s) 1131, 2995, 3933
HindII GTYRAC 3 cut(s) 1131, 2995, 3933
HindIII AAGCTT 4 cut(s) 2148, 3449, 3458, 3636
HpaII CCGG 6 cut(s) 97, 115, 2655, 4310, 4826, 4859
Hpy99I CGWCG 3 cut(s) 185, 942, 2260
HpyCH4III ACNGT 8 cut(s) 346, 490, 674, 1874, 1939, 2249, 2401, 2678
Hsp92I GRCGYC 2 cut(s) 940, 3783
HspAI GCGC 2 cut(s) 3015, 3560
Ksp22I TGATCA 1 cut(s) 2389
LmnI GCTCC 6 cut(s) 67, 1198, 3492, 3587, 4065, 4156
Lsp1109I GCAGC 8 cut(s) 769, 1654, 2191, 2881, 2903, 3074, 3669, 4171
MabI ACCWGGT 1 cut(s) 4387
MfeI CAATTG 1 cut(s) 786
MflI RGATCY 9 cut(s) 210, 373, 922, 1393, 1594, 1795, 2101, 3794, 4535
MhlI GDGCHC 6 cut(s) 2464, 3196, 4257, 4606, 4866, 4910
MlsI TGGCCA 3 cut(s) 1037, 3290, 3361
MluNI TGGCCA 3 cut(s) 1037, 3290, 3361
MlyI GAGTC 4 cut(s) 536, 803, 4087, 4791
MmeI TCCRAC 7 cut(s) 249, 2079, 2277, 2279, 2410, 3759, 4722
Mox20I TGGCCA 3 cut(s) 1037, 3290, 3361
Mph1103I ATGCAT 5 cut(s) 881, 2060, 3901, 4043, 5046
MroXI GAANNNNTTC 2 cut(s) 3540, 4568
MscI TGGCCA 3 cut(s) 1037, 3290, 3361
MslI CAYNNNNRTG 3 cut(s) 2375, 2457, 4995
Msp20I TGGCCA 3 cut(s) 1037, 3290, 3361
MspA1I CMGCKG 4 cut(s) 47, 1787, 2108, 2894
MspCI CTTAAG 2 cut(s) 3692, 3731
MspI CCGG 6 cut(s) 97, 115, 2655, 4310, 4826, 4859
MspR9I CCNGG 6 cut(s) 116, 1468, 1669, 2655, 3409, 4389
MunI CAATTG 1 cut(s) 786
Mva1269I GAATGC 4 cut(s) 881, 2060, 2867, 4450
MvaI CCWGG 4 cut(s) 1468, 1669, 3409, 4389
NciI CCSGG 2 cut(s) 116, 2655
NcoI CCATGG 3 cut(s) 1167, 2358, 4213
NheI GCTAGC 1 cut(s) 2548
NlaIV GGNNCC 8 cut(s) 63, 375, 924, 2103, 3401, 4256, 4393, 4907
NmeAIII GCCGAG 1 cut(s) 4497
NsiI ATGCAT 5 cut(s) 881, 2060, 3901, 4043, 5046
NspI RCATGY 5 cut(s) 416, 2456, 3987, 4431, 4649
NspV TTCGAA 1 cut(s) 3280
PaeI GCATGC 1 cut(s) 4649
PaeR7I CTCGAG 1 cut(s) 4358
PagI TCATGA 1 cut(s) 2370
PaqCI CACCTGC 1 cut(s) 1390
PciI ACATGT 2 cut(s) 2452, 4427
PctI GAATGC 4 cut(s) 881, 2060, 2867, 4450
PdmI GAANNNNTTC 2 cut(s) 3540, 4568
Pfl23II CGTACG 1 cut(s) 2113
PflFI GACNNNGTC 2 cut(s) 1001, 4781
PflMI CCANNNNNTGG 3 cut(s) 1436, 1637, 2728
PinAI ACCGGT 1 cut(s) 4309
PkrI GCNGC 9 cut(s) 61, 759, 1644, 2206, 2893, 2896, 3089, 3659, 4161
PleI GAGTC 4 cut(s) 535, 802, 4087, 4790
PpsI GAGTC 4 cut(s) 535, 802, 4087, 4790
PscI ACATGT 2 cut(s) 2452, 4427
PshAI GACNNNNGTC 1 cut(s) 2444
PsiI TTATAA 4 cut(s) 644, 915, 2094, 2603
Psp6I CCWGG 4 cut(s) 1466, 1667, 3407, 4387
PspEI GGTNACC 1 cut(s) 2657
PspFI CCCAGC 1 cut(s) 1179
PspGI CCWGG 4 cut(s) 1466, 1667, 3407, 4387
PspLI CGTACG 1 cut(s) 2113
PspN4I GGNNCC 8 cut(s) 63, 375, 924, 2103, 3401, 4256, 4393, 4907
PspPI GGNCC 4 cut(s) 740, 1164, 3399, 4634
PspXI VCTCGAGB 1 cut(s) 4358
PstI CTGCAG 1 cut(s) 572
PstNI CAGNNNCTG 1 cut(s) 1196
PsuI RGATCY 9 cut(s) 210, 373, 922, 1393, 1594, 1795, 2101, 3794, 4535
PsyI GACNNNGTC 2 cut(s) 1001, 4781
PvuII CAGCTG 3 cut(s) 47, 1787, 2894
RsaI GTAC 8 cut(s) 137, 2115, 2307, 3212, 3244, 4007, 4169, 4506
RsaNI GTAC 8 cut(s) 136, 2114, 2306, 3211, 3243, 4006, 4168, 4505
RseI CAYNNNNRTG 3 cut(s) 2375, 2457, 4995
SatI GCNGC 9 cut(s) 60, 758, 1643, 2205, 2892, 2895, 3088, 3658, 4160
Sau96I GGNCC 4 cut(s) 740, 1164, 3399, 4634
ScaI AGTACT 2 cut(s) 2307, 4007
SchI GAGTC 4 cut(s) 536, 803, 4087, 4791
ScrFI CCNGG 6 cut(s) 116, 1468, 1669, 2655, 3409, 4389
SduI GDGCHC 6 cut(s) 2464, 3196, 4257, 4606, 4866, 4910
SexAI ACCWGGT 1 cut(s) 4387
SfcI CTRYAG 1 cut(s) 568
Sfr274I CTCGAG 1 cut(s) 4358
SfuI TTCGAA 1 cut(s) 3280
SinI GGWCC 4 cut(s) 740, 1164, 3399, 4634
SlaI CTCGAG 1 cut(s) 4358
SmiMI CAYNNNNRTG 3 cut(s) 2375, 2457, 4995
SmlI CTYRAG 9 cut(s) 216, 2856, 3131, 3692, 3701, 3731, 4358, 4406, 4573
SmoI CTYRAG 9 cut(s) 216, 2856, 3131, 3692, 3701, 3731, 4358, 4406, 4573
SpeI ACTAGT 1 cut(s) 4615
SphI GCATGC 1 cut(s) 4649
SsiI CCGC 3 cut(s) 59, 1100, 2106
SspI AATATT 3 cut(s) 2758, 3470, 4894
StyD4I CCNGG 6 cut(s) 114, 1466, 1667, 2653, 3407, 4387
StyI CCWWGG 4 cut(s) 1167, 2358, 2404, 4213
TaaI ACNGT 8 cut(s) 346, 490, 674, 1874, 1939, 2249, 2401, 2678
TaqI TCGA 8 cut(s) 622, 1053, 1846, 1968, 2274, 3280, 3827, 4359
TaqII GACCGA 2 cut(s) 1152, 3284
TatI WGTACW 5 cut(s) 135, 2305, 4005, 4167, 4504
TauI GCSGC 1 cut(s) 62
TseI GCWGC 8 cut(s) 757, 1642, 2204, 2891, 2894, 3087, 3657, 4159
TspGWI ACGGA 4 cut(s) 753, 759, 2273, 4918
Tth111I GACNNNGTC 2 cut(s) 1001, 4781
Van91I CCANNNNNTGG 3 cut(s) 1436, 1637, 2728
Vha464I CTTAAG 2 cut(s) 3692, 3731
VneI GTGCAC 1 cut(s) 2460
VpaK11BI GGWCC 4 cut(s) 740, 1164, 3399, 4634
XbaI TCTAGA 5 cut(s) 3224, 4510, 4606, 5002, 5020
XceI RCATGY 5 cut(s) 416, 2456, 3987, 4431, 4649
XcmI CCANNNNNNNNNTGG 1 cut(s) 295
XhoI CTCGAG 1 cut(s) 4358
XmiI GTMKAC 2 cut(s) 574, 1114
XmnI GAANNNNTTC 2 cut(s) 3540, 4568
ZraI GACGTC 1 cut(s) 3784
ZrmI AGTACT 2 cut(s) 2307, 4007
Zsp2I ATGCAT 5 cut(s) 881, 2060, 3901, 4043, 5046
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.