Rw0G020380

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00915
OG0000085
Physical Location & Seq
Forward (+)
8455 .. 12113
3659 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G020380.1

Sequence Viewer

Length: 3306 bp
ATGGCCTCGATCTCCCAAACAGCCTCTGCATCTTTTCCTTCACCTGCTCCTAATTGGAAGTATGATGTCTTTTTGAGTTTCAGGGGTGTAGATACTCGCAAGGGTATTACATTCGAATTATACGATCGTCTGCACAACAGAAGAGGAATTAAAACATTCATGGATGACCAAGATCTTGAAGTAGGAGCTCTTATTTCTCTAAGACTCTTAAAAGCAATCGAAAAATCAAGGCTTGCAATTGTTGTTCTCTCCCTAAATTATGCTTCTTCGAGTTGGTGTTTGGAGGAACTTACCAAGATCTGTCAATGTATGCAAGATGATGATAGAATTCTTCCGGTTTTTTATCATGTGGATCCCTCCGATGTAAGATATCAAAAGAAGAGTTTTGAAGAAGCTTTCACTAAGCATGAAAAATCTGGGCAACAAAAAATCAAGATGCAGCAGTGGAGAGATGCTTTAAATAGGGTGACCAGTTTCTGTGGGTGGCATACACAAAATTATAAGACTGAAAGAGAGCTTGTCCATGCAATTGTGGAATCTGTGTGCAGTAAAGTCCGACCTATTAAAATTGAGTTTTCAATGTCCACAGGAGATTTTAAAGCATTTGAAGCAACAAAGCAAGCCATGAATAAGGTAATGAATGCGCTAAATGATGATGAGGTCACTGTGGTTGGGGTCTACGGAATGGGAGGCGTTGGAAAGACAACCATGGTGAAACATGTCGGTGCACAAGTGAAGAAAAGTAGACTTTTTAATCAAGTGATTATGGCTGTAGTATCACAAAACCCTGACTTGAGGAAAATTCAAGGCACATTAGCAGATTTGCTGGACTTGAAATTGGAGGATGAGACAGAAATAGGAAGAGCTAGTAGATTGGAGGAGAAGATAAAAGGAGGAACCAGGATTCTCATAATCTTGGATGACGTTTGGAATAGAATAGAATTTTCAAGCATAGGAATTCCCAGCCACCACAAACTTCAAAGATGCAATTCCAAAGTCCTACTCACCACAAGGAGATTTTCTGTATGTCATTTCATGAACAGCCATGTAAACATTCCACTCAATATCCTATCAGAAGAAGATTCTTGGAACTTGTTTGTGACAGAAGCAAGACAATCTTTTGACAAATCCACCAATTTCTATGATGTAGCAAGGATGGTGGCTAGAGAATGCGCTGGTCTACCAGTTGCACTGATAGCAGTTGCGAGGGCACTTCGACATGCAAGTTTCAATGAATGGAAAGACTCGGCTCAACGACTAAAGGCGTCTCAACCTGCCAACCCTGAAGATGAGGGAGATGTGTACGAATGCATAAAGTTAAGCTATGATTACTTGAAAACTGATGATTCAAAATCATGCTTCTTACTTTGCTGCCTGTTCCCAGAGGATTATGACATCCCAGTTGAACAGTTACTGAAGTATGGAATTGGGAAAGGAATGTTTCAAGATTCCAACATGCAAGAAGCCAGAGGCACAACACATTTAGTGGTCAAGTCCCTTAAAGCTTCTAGCTTGCTTCTGGACTCAAAAAAGGATGGATATGTAAAGATGCATGATGTCATTCGGGATGTGGCTGTATTGATTTCGCTATCTGAAGTTGGTCAACGGGTTCTGGTAAAAGCTGGTTGCGACTTAAAGCATTGGCCAATGATTAACGCGCATAGAGGCTTCTTTGCAATTTCACTAATGAAGAATGAAATCCGCAAGCTACCTGAAGAGTTGGTATGTCCAAACCTTAAGATTTTATTACTACAACATAATGCTAATTTAAATGACATACCCGAGACTTTTCTCCAAACCCTGAATGAACTAAGAGTCTTAGATCTTAGCCACACTAGTATTTCATTACCACCCCAATCATTCAGTCTCCTGACCAATCTCCAAGCTTTGTATTTAGATTGTTGCATGAAAATCATTGACTTTTCCATACTCGGAAAACTTGAGAAGCTTGAGATTCTTAGTATGAGGGAATATCCTCTTAAAGAATTGTCGAGAGAAATAGGACGCTTGACCAATCTAAGAATGCTGGATGTCAGTGATAGTTTCAGCCATGGACATATTGTTACAATTCCATCTAAAGTGATATCAAAGTTGCATAGACTAGAAGAACTGTACATGCATTGCAGATTTGGGGACTGGGGAAGTAAAGTCGAGGAAGGAGGAGAAGAGACTAATATTGGATTTGATGAGTTAACTGGCTTATCATATTTATACATTTTGACGGTTCGCATATGGGATGCAAAATGTTTGCCTAAATGTGTTGAGTTCAATCCAAATTGGGTTTACTTTGATATCTGTTTCGGTGGAGAAAGAAGAGTGCCGCACATTCAGTCTCAGAATGGTCATAAATATTCCAGATCCTTGATTCTTAACACGACCATCTGCCGCTTACCGGATTGGTTTGTCAGTGTGGTGACAAAGAAAACAGAGAAGCTACAGTTTGCAAAATGCGAAGGGTTAAATAACATTCTTGTGGAATATGACCGTGGGGGGTTATGCGGGCTGAAGCATCTCTCTATTATTGGTCCCTGTGAGAAGTTGAAAGAGCTGATGAACACAATAAGTTGGATTCCAAATAAACCTGTGTTCGAGAACTTGGAGGAGTTGCACTTGGATAGTGTGGATTGCCTAAATGAATTATGTGTTGGTGAATTACCACCTGGGTCTTTGTTTAATCTTAAGCTGTTGAATGTCTGTAATTGTCGTAACTTGGGGAATGTACTGTTGCCATCAAAACTGTTACGGAAACTACCAAACTTGGAAAAGTTGATTTGCAATGTTATGGGTAGCATGGAATATGTATTCGGGTACGAAGGGTTTGAGCCAGAAGAATCAAAATTCAGAGAGATGACATTGTTGAGTCTGTATGCTCTAAAAAGCGTATGTAACGGTCCTGCTCCTTGTACAATATTCCGGACTCTTAAAAGTTTGATCATTTACCATTGCGATCAGCTTCAAGGAAGCCTCTTCACATCTGATGTAGCTCAGTGCCTTTATCATCTGGAAGACCTTTTTGTATTCAGTTGCCCTTTCTTGAAAAAATTAATTGAAGCAAGCGAGGAAACAGTGAGCAAGAAGAAGATTGTTCTTCCAAAATTGAAGAGCTTAGTTTTGCTGATGCTTCCAAGGCTGTCTAGTGAAAGTGCTATTGGTATTGAGTCACCTTCATTGGTACATTTGTATGTCCAGTATTGCCCCAACTTTTCGAATGATGCTATTAACTTCCACAGCAAGAACCAAGTCCAACTCAATGATGAACGACAAATGAGGTTTCTATGGTCGAGATTGATGAAGAACATACCGTAA

Protein Analysis

1101

Amino Acids

125.43

Weight (kDa)

7.63

Isoelectric Point (pI)

42.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 20 - 187 6.1e-48 TIR domain
TIR_2 PF13676 23 - 119 2.1e-13 TIR domain
NB-ARC PF00931 206 - 371 1.4e-30 NB-ARC domain
LRR_8 PF13855 577 - 633 4.3e-08 Leucine rich repeat
LRR_14 PF23598 579 - 712 5.5e-13 Leucine-rich repeat region
LRR_RPS2 PF23247 866 - 1009 1e-19 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 950 - 1068 1e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 501
AarI CACCTGC 1 cut(s) 52
Acc36I ACCTGC 2 cut(s) 52, 1282
AccI GTMKAC 3 cut(s) 678, 745, 1180
AccII CGCG 1 cut(s) 1658
AccIII TCCGGA 1 cut(s) 2913
AciI CCGC 4 cut(s) 1702, 2321, 2386, 2499
AclWI GGATC 3 cut(s) 347, 360, 2352
AcoI YGGCCR 1 cut(s) 1643
AcsI RAATTY 5 cut(s) 327, 801, 941, 957, 2837
AcuI CTGAAG 5 cut(s) 1305, 1436, 1614, 1734, 2525
AcyI GRCGYC 1 cut(s) 1265
AfaI GTAC 6 cut(s) 1304, 2114, 2721, 2810, 2905, 3174
AfiI CCNNNNNNNGG 1 cut(s) 2392
AflII CTTAAG 2 cut(s) 1736, 2678
AflIII ACRYGT 1 cut(s) 718
AhlI ACTAGT 1 cut(s) 1835
AjnI CCWGG 2 cut(s) 899, 2659
AjuI GAANNNNNNNTTGG 4 cut(s) 2628, 2660, 3132, 3164
Alw21I GWGCWC 2 cut(s) 190, 730
Alw26I GTCTC 6 cut(s) 842, 1272, 1778, 1871, 2162, 2337
Alw44I GTGCAC 1 cut(s) 726
AlwI GGATC 3 cut(s) 347, 360, 2352
AlwNI CAGNNNCTG 3 cut(s) 26, 477, 1414
Ama87I CYCGRG 1 cut(s) 1781
Aor13HI TCCGGA 1 cut(s) 2913
AoxI GGCC 2 cut(s) 3, 1643
ApaLI GTGCAC 1 cut(s) 726
ApeKI GCWGC 2 cut(s) 439, 1371
ApoI RAATTY 5 cut(s) 327, 801, 941, 957, 2837
AseI ATTAAT 1 cut(s) 3044
AspLEI GCGC 3 cut(s) 646, 1175, 1660
AspS9I GGNCC 2 cut(s) 2525, 2891
AsuHPI GGTGA 7 cut(s) 33, 478, 724, 997, 2425, 2660, 3153
AsuII TTCGAA 2 cut(s) 114, 3206
AvaI CYCGRG 1 cut(s) 1781
AvaII GGWCC 2 cut(s) 2525, 2891
BaeGI GKGCMC 2 cut(s) 730, 1213
BaeI ACNNNNGTAYC 2 cut(s) 3164, 3197
BalI TGGCCA 1 cut(s) 1645
BamHI GGATCC 1 cut(s) 352
BanII GRGCYC 1 cut(s) 190
BbsI GAAGAC 1 cut(s) 3012
Bbv12I GWGCWC 2 cut(s) 190, 730
BbvI GCAGC 2 cut(s) 451, 1358
BccI CCATC 5 cut(s) 1150, 1529, 2080, 2387, 2737
BciT130I CCWGG 2 cut(s) 901, 2661
BclI TGATCA 1 cut(s) 2931
BcoDI GTCTC 6 cut(s) 842, 1272, 1778, 1871, 2162, 2337
BcuI ACTAGT 1 cut(s) 1835
BfaI CTAG 6 cut(s) 867, 1164, 1509, 1836, 2102, 3135
BfmI CTRYAG 2 cut(s) 771, 2435
BfrI CTTAAG 2 cut(s) 1736, 2678
BfuAI ACCTGC 2 cut(s) 52, 1282
BglII AGATCT 3 cut(s) 172, 297, 1822
BisI GCNGC 4 cut(s) 440, 1372, 2321, 2386
BlsI GCNGC 4 cut(s) 441, 1373, 2322, 2387
Bme1390I CCNGG 2 cut(s) 901, 2661
Bme18I GGWCC 2 cut(s) 2525, 2891
BmeT110I CYCGRG 1 cut(s) 1781
BmgT120I GGNCC 2 cut(s) 2525, 2891
BmiI GGNNCC 3 cut(s) 354, 898, 2527
BmrFI CCNGG 2 cut(s) 901, 2661
BmrI ACTGGG 2 cut(s) 1394, 2146
BmsI GCATC 9 cut(s) 38, 426, 442, 974, 1539, 2227, 2518, 3108, 3202
BmuI ACTGGG 2 cut(s) 1394, 2146
BpiI GAAGAC 1 cut(s) 3012
Bpu14I TTCGAA 2 cut(s) 114, 3206
BpuEI CTTGAG 3 cut(s) 814, 1961, 1970
BsaHI GRCGYC 1 cut(s) 1265
BsaJI CCNNGG 5 cut(s) 708, 2050, 2485, 2660, 3125
BsaWI WCCGGW 3 cut(s) 334, 2392, 2913
Bsc4I CCNNNNNNNGG 1 cut(s) 2392
Bse1I ACTGG 6 cut(s) 471, 1184, 1400, 2141, 2200, 3187
Bse3DI GCAATG 3 cut(s) 2119, 2782, 2941
BseAI TCCGGA 1 cut(s) 2913
BseBI CCWGG 2 cut(s) 901, 2661
BseDI CCNNGG 5 cut(s) 708, 2050, 2485, 2660, 3125
BseGI GGATG 9 cut(s) 169, 850, 925, 1161, 1395, 1540, 1573, 2035, 2242
BseLI CCNNNNNNNGG 1 cut(s) 2392
BseMI GCAATG 3 cut(s) 2119, 2782, 2941
BseMII CTCAG 2 cut(s) 2348, 2999
BseNI ACTGG 6 cut(s) 471, 1184, 1400, 2141, 2200, 3187
BseRI GAGGAG 3 cut(s) 893, 2175, 2615
BseSI GKGCMC 2 cut(s) 730, 1213
BseXI GCAGC 2 cut(s) 451, 1358
BseYI CCCAGC 1 cut(s) 962
BsgI GTGCAG 2 cut(s) 116, 565
Bsh1236I CGCG 1 cut(s) 1658
Bsh1285I CGRYCG 1 cut(s) 127
BshFI GGCC 2 cut(s) 5, 1645
BsiEI CGRYCG 1 cut(s) 127
BsiHKAI GWGCWC 2 cut(s) 190, 730
BsiHKCI CYCGRG 1 cut(s) 1781
BsiSI CCGG 3 cut(s) 335, 2393, 2914
BslFI GGGAC 3 cut(s) 1480, 2147, 2511
BslI CCNNNNNNNGG 1 cut(s) 2392
BsmAI GTCTC 6 cut(s) 842, 1272, 1778, 1871, 2162, 2337
BsmBI CGTCTC 1 cut(s) 1272
BsmFI GGGAC 3 cut(s) 1480, 2147, 2511
BsmI GAATGC 4 cut(s) 646, 1175, 1313, 2028
BsnI GGCC 2 cut(s) 5, 1645
BsoBI CYCGRG 1 cut(s) 1781
Bsp119I TTCGAA 2 cut(s) 114, 3206
Bsp1286I GDGCHC 3 cut(s) 190, 730, 1213
Bsp13I TCCGGA 1 cut(s) 2913
Bsp1407I TGTACA 2 cut(s) 2112, 2903
Bsp143I GATC 9 cut(s) 9, 124, 172, 297, 352, 1822, 2357, 2931, 2947
Bsp19I CCATGG 2 cut(s) 708, 2050
BspACI CCGC 4 cut(s) 1702, 2321, 2386, 2499
BspANI GGCC 2 cut(s) 5, 1645
BspCNI CTCAG 2 cut(s) 2347, 2998
BspEI TCCGGA 1 cut(s) 2913
BspFNI CGCG 1 cut(s) 1658
BspHI TCATGA 1 cut(s) 1035
BspLI GGNNCC 3 cut(s) 354, 898, 2527
BspMI ACCTGC 2 cut(s) 52, 1282
BspPI GGATC 3 cut(s) 347, 360, 2352
BspQI GCTCTTC 2 cut(s) 856, 3095
BspT104I TTCGAA 2 cut(s) 114, 3206
BspTI CTTAAG 2 cut(s) 1736, 2678
BsrDI GCAATG 3 cut(s) 2119, 2782, 2941
BsrGI TGTACA 2 cut(s) 2112, 2903
BsrI ACTGG 6 cut(s) 471, 1184, 1400, 2141, 2200, 3187
BssECI CCNNGG 5 cut(s) 708, 2050, 2485, 2660, 3125
BssMI GATC 9 cut(s) 9, 124, 172, 297, 352, 1822, 2357, 2931, 2947
BssNI GRCGYC 1 cut(s) 1265
BssT1I CCWWGG 3 cut(s) 708, 2050, 3125
Bst2UI CCWGG 2 cut(s) 901, 2661
Bst6I CTCTTC 8 cut(s) 136, 374, 856, 1710, 2160, 2308, 2972, 3095
BstACI GRCGYC 1 cut(s) 1265
BstAFI CTTAAG 2 cut(s) 1736, 2678
BstAUI TGTACA 2 cut(s) 2112, 2903
BstBI TTCGAA 2 cut(s) 114, 3206
BstC8I GCNNGC 6 cut(s) 234, 621, 1514, 1706, 2501, 3055
BstDSI CCRYGG 3 cut(s) 708, 2050, 2485
BstEII GGTNACC 1 cut(s) 466
BstF5I GGATG 9 cut(s) 169, 850, 925, 1161, 1395, 1540, 1573, 2035, 2242
BstFNI CGCG 1 cut(s) 1658
BstHHI GCGC 3 cut(s) 646, 1175, 1660
BstKTI GATC 9 cut(s) 12, 127, 175, 300, 355, 1825, 2360, 2934, 2950
BstMAI GTCTC 6 cut(s) 842, 1272, 1778, 1871, 2162, 2337
BstMBI GATC 9 cut(s) 9, 124, 172, 297, 352, 1822, 2357, 2931, 2947
BstMCI CGRYCG 1 cut(s) 127
BstMWI GCNNNNNNNGC 3 cut(s) 608, 1196, 3127
BstNI CCWGG 2 cut(s) 901, 2661
BstNSI RCATGY 4 cut(s) 722, 1223, 1459, 2119
BstPI GGTNACC 1 cut(s) 466
BstSCI CCNGG 2 cut(s) 899, 2659
BstSFI CTRYAG 2 cut(s) 771, 2435
BstSLI GKGCMC 2 cut(s) 730, 1213
BstUI CGCG 1 cut(s) 1658
BstV1I GCAGC 2 cut(s) 451, 1358
BstV2I GAAGAC 1 cut(s) 3012
BstX2I RGATCY 5 cut(s) 172, 297, 352, 1822, 2357
BstYI RGATCY 5 cut(s) 172, 297, 352, 1822, 2357
BsuRI GGCC 2 cut(s) 5, 1645
BtgI CCRYGG 3 cut(s) 708, 2050, 2485
BtsCI GGATG 9 cut(s) 169, 850, 925, 1161, 1395, 1540, 1573, 2035, 2242
BtsI GCAGTG 1 cut(s) 449
BtsIMutI CAGTG 7 cut(s) 449, 663, 1190, 2041, 2413, 2993, 3072
BveI ACCTGC 2 cut(s) 52, 1282
Cac8I GCNNGC 6 cut(s) 234, 621, 1514, 1706, 2501, 3055
CaiI CAGNNNCTG 3 cut(s) 26, 477, 1414
CciI TCATGA 1 cut(s) 1035
CfoI GCGC 3 cut(s) 646, 1175, 1660
Cfr13I GGNCC 2 cut(s) 2525, 2891
CseI GACGC 2 cut(s) 1254, 2013
Csp6I GTAC 6 cut(s) 1303, 2113, 2720, 2809, 2904, 3173
CspCI CAANNNNNGTGG 2 cut(s) 1140, 1175
CviQI GTAC 6 cut(s) 1303, 2113, 2720, 2809, 2904, 3173
DpnI GATC 9 cut(s) 11, 126, 174, 299, 354, 1824, 2359, 2933, 2949
DpnII GATC 9 cut(s) 9, 124, 172, 297, 352, 1822, 2357, 2931, 2947
DraI TTTAAA 3 cut(s) 459, 598, 1770
EaeI YGGCCR 1 cut(s) 1643
Eam1104I CTCTTC 8 cut(s) 136, 374, 856, 1710, 2160, 2308, 2972, 3095
EarI CTCTTC 8 cut(s) 136, 374, 856, 1710, 2160, 2308, 2972, 3095
Ecl136II GAGCTC 1 cut(s) 188
Eco130I CCWWGG 3 cut(s) 708, 2050, 3125
Eco24I GRGCYC 1 cut(s) 190
Eco32I GATATC 3 cut(s) 371, 2085, 2293
Eco47I GGWCC 2 cut(s) 2525, 2891
Eco53kI GAGCTC 1 cut(s) 188
Eco57I CTGAAG 5 cut(s) 1305, 1436, 1614, 1734, 2525
Eco88I CYCGRG 1 cut(s) 1781
Eco91I GGTNACC 1 cut(s) 466
EcoICRI GAGCTC 1 cut(s) 188
EcoO65I GGTNACC 1 cut(s) 466
EcoRI GAATTC 2 cut(s) 327, 957
EcoRII CCWGG 2 cut(s) 899, 2659
EcoRV GATATC 3 cut(s) 371, 2085, 2293
EcoT14I CCWWGG 3 cut(s) 708, 2050, 3125
EcoT22I ATGCAT 3 cut(s) 1313, 1554, 2121
EcoT38I GRGCYC 1 cut(s) 190
ErhI CCWWGG 3 cut(s) 708, 2050, 3125
Esp3I CGTCTC 1 cut(s) 1272
FalI AAGNNNNNCTT 2 cut(s) 1102, 1134
FaqI GGGAC 3 cut(s) 1480, 2147, 2511
FauI CCCGC 1 cut(s) 2492
FauNDI CATATG 1 cut(s) 2231
FbaI TGATCA 1 cut(s) 2931
FblI GTMKAC 3 cut(s) 678, 745, 1180
Fnu4HI GCNGC 4 cut(s) 440, 1372, 2321, 2386
FokI GGATG 9 cut(s) 176, 857, 932, 1168, 1382, 1547, 1580, 2042, 2249
FriOI GRGCYC 1 cut(s) 190
Fsp4HI GCNGC 4 cut(s) 440, 1372, 2321, 2386
FspBI CTAG 6 cut(s) 867, 1164, 1509, 1836, 2102, 3135
GlaI GCGC 3 cut(s) 645, 1174, 1659
GluI GCNGC 4 cut(s) 440, 1372, 2321, 2386
GsaI CCCAGC 1 cut(s) 966
HaeIII GGCC 2 cut(s) 5, 1645
HapII CCGG 3 cut(s) 335, 2393, 2914
HgaI GACGC 2 cut(s) 1254, 2013
HhaI GCGC 3 cut(s) 646, 1175, 1660
Hin1I GRCGYC 1 cut(s) 1265
Hin6I GCGC 3 cut(s) 644, 1173, 1658
HinP1I GCGC 3 cut(s) 644, 1173, 1658
HincII GTYRAC 2 cut(s) 1604, 2193
HindII GTYRAC 2 cut(s) 1604, 2193
HindIII AAGCTT 4 cut(s) 393, 1503, 1884, 1946
HpaI GTTAAC 1 cut(s) 2193
HpaII CCGG 3 cut(s) 335, 2393, 2914
HphI GGTGA 7 cut(s) 33, 478, 724, 997, 2425, 2660, 3153
Hpy188I TCNGA 8 cut(s) 361, 557, 1075, 1594, 1934, 2337, 2843, 2977
HpyAV CCTTC 5 cut(s) 48, 2150, 2447, 2807, 3174
HpyCH4IV ACGT 1 cut(s) 924
HpyF10VI GCNNNNNNNGC 3 cut(s) 608, 1196, 3127
HpySE526I ACGT 1 cut(s) 924
Hsp92I GRCGYC 1 cut(s) 1265
HspAI GCGC 3 cut(s) 644, 1173, 1658
Kpn2I TCCGGA 1 cut(s) 2913
Ksp22I TGATCA 1 cut(s) 2931
KspAI GTTAAC 1 cut(s) 2193
Kzo9I GATC 9 cut(s) 9, 124, 172, 297, 352, 1822, 2357, 2931, 2947
LguI GCTCTTC 2 cut(s) 856, 3095
LmnI GCTCC 3 cut(s) 52, 185, 2902
Lsp1109I GCAGC 2 cut(s) 451, 1358
LweI GCATC 9 cut(s) 38, 426, 442, 974, 1539, 2227, 2518, 3108, 3202
MaeI CTAG 6 cut(s) 867, 1164, 1509, 1836, 2102, 3135
MaeII ACGT 1 cut(s) 924
MalI GATC 9 cut(s) 11, 126, 174, 299, 354, 1824, 2359, 2933, 2949
MboI GATC 9 cut(s) 9, 124, 172, 297, 352, 1822, 2357, 2931, 2947
MfeI CAATTG 2 cut(s) 237, 528
MflI RGATCY 5 cut(s) 172, 297, 352, 1822, 2357
MhlI GDGCHC 3 cut(s) 190, 730, 1213
MlsI TGGCCA 1 cut(s) 1645
MluNI TGGCCA 1 cut(s) 1645
MlyI GAGTC 7 cut(s) 198, 1238, 1517, 1824, 2869, 2911, 3167
MmeI TCCRAC 5 cut(s) 580, 676, 1476, 2546, 3268
Mox20I TGGCCA 1 cut(s) 1645
Mph1103I ATGCAT 3 cut(s) 1313, 1554, 2121
MroI TCCGGA 1 cut(s) 2913
MscI TGGCCA 1 cut(s) 1645
MslI CAYNNNNRTG 3 cut(s) 723, 2471, 3180
Msp20I TGGCCA 1 cut(s) 1645
MspCI CTTAAG 2 cut(s) 1736, 2678
MspI CCGG 3 cut(s) 335, 2393, 2914
MspR9I CCNGG 2 cut(s) 901, 2661
MunI CAATTG 2 cut(s) 237, 528
Mva1269I GAATGC 4 cut(s) 646, 1175, 1313, 2028
MvaI CCWGG 2 cut(s) 901, 2661
MvnI CGCG 1 cut(s) 1658
MwoI GCNNNNNNNGC 3 cut(s) 608, 1196, 3127
NcoI CCATGG 2 cut(s) 708, 2050
NdeI CATATG 1 cut(s) 2231
NdeII GATC 9 cut(s) 9, 124, 172, 297, 352, 1822, 2357, 2931, 2947
NlaIV GGNNCC 3 cut(s) 354, 898, 2527
NmeAIII GCCGAG 1 cut(s) 1226
NmuCI GTSAC 5 cut(s) 466, 661, 1099, 2413, 3159
NsiI ATGCAT 3 cut(s) 1313, 1554, 2121
NspI RCATGY 4 cut(s) 722, 1223, 1459, 2119
NspV TTCGAA 2 cut(s) 114, 3206
PagI TCATGA 1 cut(s) 1035
PaqCI CACCTGC 1 cut(s) 52
PciI ACATGT 1 cut(s) 718
PciSI GCTCTTC 2 cut(s) 856, 3095
PcsI WCGNNNNNNNCGW 1 cut(s) 120
PctI GAATGC 4 cut(s) 646, 1175, 1313, 2028
PfeI GAWTC 9 cut(s) 536, 904, 1082, 1346, 1448, 1954, 2365, 2569, 2831
PkrI GCNGC 4 cut(s) 441, 1373, 2322, 2387
Ple19I CGATCG 1 cut(s) 127
PleI GAGTC 7 cut(s) 198, 1238, 1517, 1823, 2868, 2911, 3166
PpsI GAGTC 7 cut(s) 198, 1238, 1517, 1823, 2868, 2911, 3166
PscI ACATGT 1 cut(s) 718
PshBI ATTAAT 1 cut(s) 3044
PsiI TTATAA 1 cut(s) 501
Psp124BI GAGCTC 1 cut(s) 190
Psp6I CCWGG 2 cut(s) 899, 2659
PspEI GGTNACC 1 cut(s) 466
PspFI CCCAGC 1 cut(s) 962
PspGI CCWGG 2 cut(s) 899, 2659
PspN4I GGNNCC 3 cut(s) 354, 898, 2527
PspPI GGNCC 2 cut(s) 2525, 2891
PstNI CAGNNNCTG 3 cut(s) 26, 477, 1414
PsuI RGATCY 5 cut(s) 172, 297, 352, 1822, 2357
PvuI CGATCG 1 cut(s) 127
RsaI GTAC 6 cut(s) 1304, 2114, 2721, 2810, 2905, 3174
RsaNI GTAC 6 cut(s) 1303, 2113, 2720, 2809, 2904, 3173
RseI CAYNNNNRTG 3 cut(s) 723, 2471, 3180
SacI GAGCTC 1 cut(s) 190
SapI GCTCTTC 2 cut(s) 856, 3095
SatI GCNGC 4 cut(s) 440, 1372, 2321, 2386
Sau3AI GATC 9 cut(s) 9, 124, 172, 297, 352, 1822, 2357, 2931, 2947
Sau96I GGNCC 2 cut(s) 2525, 2891
SchI GAGTC 7 cut(s) 198, 1238, 1517, 1824, 2869, 2911, 3167
ScrFI CCNGG 2 cut(s) 901, 2661
SduI GDGCHC 3 cut(s) 190, 730, 1213
SfaNI GCATC 9 cut(s) 38, 426, 442, 974, 1539, 2227, 2518, 3108, 3202
SfcI CTRYAG 2 cut(s) 771, 2435
SfuI TTCGAA 2 cut(s) 114, 3206
SinI GGWCC 2 cut(s) 2525, 2891
SmiI ATTTAAAT 1 cut(s) 1770
SmiMI CAYNNNNRTG 3 cut(s) 723, 2471, 3180
SmlI CTYRAG 5 cut(s) 793, 1736, 1940, 1949, 2678
SmoI CTYRAG 5 cut(s) 793, 1736, 1940, 1949, 2678
SpeI ACTAGT 1 cut(s) 1835
SsiI CCGC 4 cut(s) 1702, 2321, 2386, 2499
SspI AATATT 3 cut(s) 2176, 2351, 2910
SspMI CTAG 6 cut(s) 867, 1164, 1509, 1836, 2102, 3135
SstI GAGCTC 1 cut(s) 190
StyD4I CCNGG 2 cut(s) 899, 2659
StyI CCWWGG 3 cut(s) 708, 2050, 3125
SwaI ATTTAAAT 1 cut(s) 1770
TaiI ACGT 1 cut(s) 927
TatI WGTACW 3 cut(s) 2112, 2719, 2903
TauI GCSGC 2 cut(s) 2323, 2388
TfiI GAWTC 9 cut(s) 536, 904, 1082, 1346, 1448, 1954, 2365, 2569, 2831
TscAI CASTG 7 cut(s) 449, 670, 1197, 2041, 2413, 2993, 3072
TseFI GTSAC 5 cut(s) 466, 661, 1099, 2413, 3159
TseI GCWGC 2 cut(s) 439, 1371
Tsp45I GTSAC 5 cut(s) 466, 661, 1099, 2413, 3159
TspGWI ACGGA 2 cut(s) 696, 2758
TspRI CASTG 7 cut(s) 449, 670, 1197, 2041, 2413, 2993, 3072
Vha464I CTTAAG 2 cut(s) 1736, 2678
VneI GTGCAC 1 cut(s) 726
VpaK11BI GGWCC 2 cut(s) 2525, 2891
VspI ATTAAT 1 cut(s) 3044
XapI RAATTY 5 cut(s) 327, 801, 941, 957, 2837
XceI RCATGY 4 cut(s) 722, 1223, 1459, 2119
XmiI GTMKAC 3 cut(s) 678, 745, 1180
XspI CTAG 6 cut(s) 867, 1164, 1509, 1836, 2102, 3135
Zsp2I ATGCAT 3 cut(s) 1313, 1554, 2121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.