Rw6G007030
WRKY Family

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000085
Physical Location & Seq
Reverse (-)
11369600 .. 11374282
4683 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G007030.1

Sequence Viewer

Length: 3144 bp
ATGGCGGAGATTATTTTGCATCCAATAATGAAACTTGGTGAGTGGATGTGGACTCATGTGAAGCGTGGGATGAGTTACTCGGTTCATTACAAAAAAAAATTAGAGTCTCTTGACCTTCAGTTTAAAAAGCTTGAGGAGATGCGGGATGGTCATAAGGAATCTGTGGATATTGCTGAAAATAGTGGAGACAGAATCAAGCCTGAGGTTCAGACATGGCTAAGAGATGCTGATAATGCCATGACAGATGTGAGACAATTGAATGGTGAAGCTGGAGCAGGTGAAAAGTGCTTTATAGGATGGTGTCCGGATTTGAAATGGCGTTACAATTTGGGGAAGAAAGCATCGAAGCTGACAACAACGCTAGACAAGCTTGTCAAAGAGGCAAAATTTGAGACTGTTTCACTTGAAGTTCGACGAGCACCTGAAGTTCAGCACTTGATCTCCACTGAAGGATTTGAAGCATTTGAAGCAACTAGACAAGCCATGGATAGAGTCATGAACGCGCTGGAGAAAGACGAGGTCACAGCTATTGGTGTCTATGGAATGGGTGGCATAGGGAAAACAACTTTGGTGAAACACGTTGGTGCACAAGTGTGTCATCAAGATGGGCTCTTTGATCGTATGATTATGGCTGTTGTCTCTCAGAACCCTGTGGTGATCCAAATTCAAGACCAACTTAAAGATATGCTGGCCTTGAAGTTGCATGAAACAACAGAAATGGCAAGAGCAGCTAGACTGAGAGAGAGGATTATGAGAGAAAAGAAGATCCTCATAATCTTGGACGATATTTGGGACACAATAGATTTGTCTATCATAGGAATTCCTAGCCACGAGCAGCTTGGAAGATGCGGTTCCAAAGTCATCATTACCACAAGGATATTCCACATTTGTCATACCATGGGGAGTCAAGTTAAGATCCCTCTCAACCGATTATCTGAAGATGATTCTTGGAAGTTGTTTGTGAGGCATGCTAGAAGGCCTTTTGAGTCAACCAATCTCTGTGAGGTAGCAAAGCAAGTAGCTAGAGAGTGTGGTGGCCTCCCAGTTGCATTGATCACAGTTGCAAGGGCACTTGGAGATAAAGACCAGTTGGAAGAATGGCAAGACGCAGCTCAACGACTAGAGATTTCCCAACCTGCAAGTCTTGATGACGACGGAAGTGTGCTCAAATGTATAAGGTTAAGTTATGATTTCTTGAAAAGCAAGGATGCAAAATCAGTCTTCTTGCTTTGTTGCCTCTTTCCGGAAGACTATGATATCCAAATCGAGGACTTGATCAAGTATGGACTTGGGAAAGGGTTGTTTCGGGATGCTGCCACATTTGAAGGAGCCAGAAGTAGAGCGCATTCGGTGATCAACTACCTTAAATCTTCCAACTTGCTTTTGGACAGTAAAAGGGGTGAATGCGTAAAGATGCATGATGTCATTCGGGATATGGCCATGTTAATTGCATCATCCAAATATGGTGATGGAATTTTGGTTAAATCTCGGATTCTATTAAGGGTTTGGCCAAATAATGCATATGAAGGCTACTCTGCAATCTCTTTAATGGAGAACGATATTCGGAAGCTTCCTGAAGAGTTGGTATGTCCAAAACTCCAGATTTTATTATTGCAAGATAACAAAAATATGAAGGAGATCCCAGAAACATTTTTTGAAAGTCTGAATGAGTTAAAGGTCTTAGATCTCAGTCGCTGTGGTATGTCGATCTTACCACACTCTTTGAGTCTGCGAACCAACCTCCAGGCATTATATTTAGATAATTGCCGGTCAATAATGTATGTTTCTCTACTCGGAAAACTGAAGAAACTTGAAATTCTTAGCCTGAGACATTCAACCCTTGAAGAATTACCTGCAGAAATAGGAAATTTGGCTAACCTAAGAATGCTTGACCTTAGTGGGAGTGGGTCCATTAAGAGAATTTCATCCAATTTGATATCAAGGTTGTCTAGGTTAGAAGAACTATACATGCAAGGTAGTTTTCAGGACTGGGGATGTAAAGTTGAGGGAGGAGGAGAAGAAACCAATGTTGGATTTGATGAGTTGATTTGCCTGTCATACTTGAATATTTTGGAGGTTGACATAATTGATCCAAAGTGTTTGCCAAACCATGTTAGCTTTAATCCCAATTGGGTAAAGTTTCACATAGGTATCAGAGGAAACGCACGTGGGCCTCTGGTTATCTCTCTTTGGGAGCTTCTTTGGATAAATATTTATAAGTTAGATTCAAGGACATTGAACCTTAACACAAGCATTACCACCTTGCCCGGTTGGTTTAATAGTGCTGTTACCCAGAGAGCCAAGAAAGTTAGGTACACAAAGTGTCAGGGCTTAAATAACATTCTAGTGGAATATGACCATGGAAATTTACTGACACTTCAGTCTCTAGTTATCCAAGATTGCTATCAAGTTGTAACTTTGATAGACACAATAACAAGGGTTCCAATCACGAATGAACCTGTGTTTGAGAATCTAGAATTCTTGTGGGTGGTGAATTTGAATAATTTGAAAGAGTTGTGTGTTGGTGAATTACCACAAGGGTCTTTGGGCAATCTGAAGTATTTGGAGGCGAACAATTGTAGTAACTTGTTGAATCCACTTCTTCCATCTAATTTGTTGAAAAGATTGCAAAATCTGGAAAAACTTGTTATTTCAGATGTATCACGTGCGGAATATGTGTTTCGGTCGGAAGATTTTGGAGAAACACAAATTGAGTTGAGAAAATTTAGACATCTTAGATTGTGCCGTCTACAGCAACTGGAAAACATATGGAGAGGTCCTGTTGGTTATGCATTCTTCTGTAATATTCGACATTTGGAAGTTGACAGCTGTGACAAATTGAAATATCTCTTCACATCTGATGTATCTCAAAGTATCATGCAATTGGTGTATCTTCGTGTAGAGTCCTGCGATTGTTTGGAGATGATCATTGCACCAGGCGAGGGAACAGTGAATAAAATAGTTTTGCCACAGTTAAATTACTTATATTTGGAGGATCTTCCACAGCTCATAAGCTTCTACACCGGAACTAATGGGATCGAGTGTCCGTCATTGGAAATCTTATCTGTGCATGAGTGCCCACAGTTTCCAACCTCTACTTACGACTTCCACAGCAGAAACCGAGTCAATGTCCGTCCAAGGTGA

Protein Analysis

1047

Amino Acids

119.21

Weight (kDa)

6.34

Isoelectric Point (pI)

42.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 160 - 323 1.6e-31 NB-ARC domain
LRR_8 PF13855 531 - 590 8.2e-07 Leucine rich repeat
LRR_14 PF23598 566 - 661 1.8e-09 Leucine-rich repeat region
LRR_8 PF13855 603 - 659 2.2e-06 Leucine rich repeat
LRR_RPS2 PF23247 830 - 971 1.2e-27 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2217
AarI CACCTGC 1 cut(s) 266
AasI GACNNNNNNGTC 2 cut(s) 371, 2380
Acc36I ACCTGC 3 cut(s) 266, 1144, 1861
AccI GTMKAC 1 cut(s) 2749
AccII CGCG 1 cut(s) 503
AccIII TCCGGA 2 cut(s) 304, 1243
AciI CCGC 4 cut(s) 5, 142, 849, 2669
AclWI GGATC 7 cut(s) 652, 760, 910, 1633, 2084, 3003, 3044
AcoI YGGCCR 2 cut(s) 1437, 1508
AcuI CTGAAG 8 cut(s) 101, 444, 468, 957, 1596, 1823, 2363, 2576
AcvI CACGTG 2 cut(s) 2168, 2666
AdeI CACNNNGTG 1 cut(s) 2322
AfaI GTAC 1 cut(s) 2315
AfiI CCNNNNNNNGG 3 cut(s) 1243, 1267, 2942
AflIII ACRYGT 1 cut(s) 577
AjnI CCWGG 2 cut(s) 1743, 2935
AjuI GAANNNNNNNTTGG 4 cut(s) 551, 583, 2013, 2045
AleI CACNNNNGTG 2 cut(s) 582, 592
Alw21I GWGCWC 3 cut(s) 421, 589, 1167
Alw26I GTCTC 7 cut(s) 111, 180, 244, 386, 643, 1822, 2388
Alw44I GTGCAC 1 cut(s) 585
AlwI GGATC 7 cut(s) 652, 760, 910, 1633, 2084, 3003, 3044
AlwNI CAGNNNCTG 2 cut(s) 1695, 2758
Aor13HI TCCGGA 2 cut(s) 304, 1243
AoxI GGCC 6 cut(s) 690, 977, 1036, 1437, 1508, 2171
ApaLI GTGCAC 1 cut(s) 585
ApeKI GCWGC 4 cut(s) 728, 835, 1109, 1313
AspLEI GCGC 2 cut(s) 505, 1345
AspS9I GGNCC 3 cut(s) 1908, 2171, 2777
AsuC2I CCSGG 1 cut(s) 2268
AvaII GGWCC 2 cut(s) 1908, 2777
AxyI CCTNAGG 1 cut(s) 201
BaeGI GKGCMC 3 cut(s) 589, 1072, 3080
BaeI ACNNNNGTAYC 2 cut(s) 2134, 2167
BalI TGGCCA 2 cut(s) 1439, 1510
BanII GRGCYC 1 cut(s) 612
BauI CACGAG 1 cut(s) 830
BbrPI CACGTG 2 cut(s) 2168, 2666
BbsI GAAGAC 2 cut(s) 1213, 1254
Bbv12I GWGCWC 3 cut(s) 421, 589, 1167
BbvI GCAGC 4 cut(s) 740, 847, 1121, 1300
BccI CCATC 5 cut(s) 140, 291, 599, 1463, 2614
BceAI ACGGC 1 cut(s) 2730
BciT130I CCWGG 2 cut(s) 1745, 2937
BclI TGATCA 4 cut(s) 1053, 1275, 1353, 2925
BcnI CCSGG 1 cut(s) 2268
BcoDI GTCTC 7 cut(s) 111, 180, 244, 386, 643, 1822, 2388
BfmI CTRYAG 2 cut(s) 1854, 2750
BfuAI ACCTGC 3 cut(s) 266, 1144, 1861
BglII AGATCT 1 cut(s) 1684
BisI GCNGC 4 cut(s) 729, 836, 1110, 1314
BlsI GCNGC 4 cut(s) 730, 837, 1111, 1315
Bme1390I CCNGG 3 cut(s) 1745, 2268, 2937
Bme18I GGWCC 2 cut(s) 1908, 2777
BmgT120I GGNCC 3 cut(s) 1908, 2171, 2777
BmiI GGNNCC 4 cut(s) 853, 1330, 1909, 2442
BmrFI CCNGG 3 cut(s) 1745, 2268, 2937
BmrI ACTGGG 2 cut(s) 1037, 1999
BmsI GCATC 9 cut(s) 28, 129, 214, 350, 836, 1198, 1300, 1404, 1460
BmuI ACTGGG 2 cut(s) 1037, 1999
BpiI GAAGAC 2 cut(s) 1213, 1254
BpmI CTGGAG 4 cut(s) 291, 527, 1583, 1727
BpuEI CTTGAG 1 cut(s) 152
BpuMI CCSGG 1 cut(s) 2268
BsaAI YACGTR 2 cut(s) 2168, 2666
BsaBI GATNNNNATC 1 cut(s) 2403
BsaJI CCNNGG 4 cut(s) 483, 897, 2359, 3137
BsaWI WCCGGW 3 cut(s) 304, 1243, 3023
BsaXI ACNNNNNCTCC 2 cut(s) 425, 455
Bsc4I CCNNNNNNNGG 3 cut(s) 1243, 1267, 2942
Bse118I RCCGGY 1 cut(s) 1767
Bse1I ACTGG 4 cut(s) 1043, 1087, 1994, 2763
Bse21I CCTNAGG 1 cut(s) 201
Bse3DI GCAATG 1 cut(s) 2928
Bse8I GATNNNNATC 1 cut(s) 2403
BseAI TCCGGA 2 cut(s) 304, 1243
BseBI CCWGG 2 cut(s) 1745, 2937
BseDI CCNNGG 4 cut(s) 483, 897, 2359, 3137
BseJI GATNNNNATC 1 cut(s) 2403
BseLI CCNNNNNNNGG 3 cut(s) 1243, 1267, 2942
BseMI GCAATG 1 cut(s) 2928
BseMII CTCAG 5 cut(s) 192, 656, 728, 1702, 1817
BseNI ACTGG 4 cut(s) 1043, 1087, 1994, 2763
BseRI GAGGAG 3 cut(s) 149, 2025, 2028
BseSI GKGCMC 3 cut(s) 589, 1072, 3080
BseXI GCAGC 4 cut(s) 740, 847, 1121, 1300
Bsh1236I CGCG 1 cut(s) 503
Bsh1285I CGRYCG 1 cut(s) 2687
BshFI GGCC 6 cut(s) 692, 979, 1038, 1439, 1510, 2173
BsiEI CGRYCG 1 cut(s) 2687
BsiHKAI GWGCWC 3 cut(s) 421, 589, 1167
BsiSI CCGG 5 cut(s) 305, 1244, 1768, 2268, 3024
BslFI GGGAC 1 cut(s) 806
BslI CCNNNNNNNGG 3 cut(s) 1243, 1267, 2942
BsmAI GTCTC 7 cut(s) 111, 180, 244, 386, 643, 1822, 2388
BsmFI GGGAC 1 cut(s) 806
BsmI GAATGC 4 cut(s) 1345, 1409, 1890, 2792
BsnI GGCC 6 cut(s) 692, 979, 1038, 1439, 1510, 2173
Bsp1286I GDGCHC 6 cut(s) 421, 589, 612, 1072, 1167, 3080
Bsp13I TCCGGA 2 cut(s) 304, 1243
Bsp19I CCATGG 3 cut(s) 483, 897, 2359
BspACI CCGC 4 cut(s) 5, 142, 849, 2669
BspANI GGCC 6 cut(s) 692, 979, 1038, 1439, 1510, 2173
BspCNI CTCAG 5 cut(s) 193, 655, 729, 1701, 1818
BspEI TCCGGA 2 cut(s) 304, 1243
BspFNI CGCG 1 cut(s) 503
BspHI TCATGA 1 cut(s) 495
BspLI GGNNCC 4 cut(s) 853, 1330, 1909, 2442
BspMAI CTGCAG 1 cut(s) 1858
BspMI ACCTGC 3 cut(s) 266, 1144, 1861
BspPI GGATC 7 cut(s) 652, 760, 910, 1633, 2084, 3003, 3044
BsrDI GCAATG 1 cut(s) 2928
BsrFI RCCGGY 1 cut(s) 1767
BsrI ACTGG 4 cut(s) 1043, 1087, 1994, 2763
BssAI RCCGGY 1 cut(s) 1767
BssECI CCNNGG 4 cut(s) 483, 897, 2359, 3137
BssSI CACGAG 1 cut(s) 830
BssT1I CCWWGG 4 cut(s) 483, 897, 2359, 3137
Bst2BI CACGAG 1 cut(s) 830
Bst2UI CCWGG 2 cut(s) 1745, 2937
Bst4CI ACNGT 6 cut(s) 397, 1060, 1391, 2950, 2973, 3084
Bst6I CTCTTC 2 cut(s) 1572, 2855
BstBAI YACGTR 2 cut(s) 2168, 2666
BstC8I GCNNGC 2 cut(s) 690, 969
BstDSI CCRYGG 3 cut(s) 483, 897, 2359
BstFNI CGCG 1 cut(s) 503
BstHHI GCGC 2 cut(s) 505, 1345
BstMAI GTCTC 7 cut(s) 111, 180, 244, 386, 643, 1822, 2388
BstMCI CGRYCG 1 cut(s) 2687
BstMWI GCNNNNNNNGC 4 cut(s) 233, 367, 467, 728
BstNI CCWGG 2 cut(s) 1745, 2937
BstNSI RCATGY 2 cut(s) 971, 1972
BstSCI CCNGG 3 cut(s) 1743, 2266, 2935
BstSFI CTRYAG 2 cut(s) 1854, 2750
BstSLI GKGCMC 3 cut(s) 589, 1072, 3080
BstUI CGCG 1 cut(s) 503
BstV1I GCAGC 4 cut(s) 740, 847, 1121, 1300
BstV2I GAAGAC 2 cut(s) 1213, 1254
BstX2I RGATCY 5 cut(s) 765, 915, 1638, 1684, 2995
BstYI RGATCY 5 cut(s) 765, 915, 1638, 1684, 2995
Bsu36I CCTNAGG 1 cut(s) 201
BsuRI GGCC 6 cut(s) 692, 979, 1038, 1439, 1510, 2173
BtgI CCRYGG 3 cut(s) 483, 897, 2359
BtsIMutI CAGTG 2 cut(s) 444, 2955
BveI ACCTGC 3 cut(s) 266, 1144, 1861
Cac8I GCNNGC 2 cut(s) 690, 969
CaiI CAGNNNCTG 2 cut(s) 1695, 2758
CciI TCATGA 1 cut(s) 495
CfoI GCGC 2 cut(s) 505, 1345
Cfr10I RCCGGY 1 cut(s) 1767
Cfr13I GGNCC 3 cut(s) 1908, 2171, 2777
CseI GACGC 1 cut(s) 1115
Csp6I GTAC 1 cut(s) 2314
CspCI CAANNNNNGTGG 2 cut(s) 1704, 1739
CviQI GTAC 1 cut(s) 2314
DraI TTTAAA 1 cut(s) 124
DraIII CACNNNGTG 1 cut(s) 2322
DrdI GACNNNNNNGTC 2 cut(s) 371, 2380
DseDI GACNNNNNNGTC 2 cut(s) 371, 2380
EaeI YGGCCR 2 cut(s) 1437, 1508
Eam1104I CTCTTC 2 cut(s) 1572, 2855
EarI CTCTTC 2 cut(s) 1572, 2855
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 4 cut(s) 483, 897, 2359, 3137
Eco147I AGGCCT 1 cut(s) 979
Eco24I GRGCYC 1 cut(s) 612
Eco32I GATATC 2 cut(s) 1258, 1938
Eco47I GGWCC 2 cut(s) 1908, 2777
Eco57I CTGAAG 8 cut(s) 101, 444, 468, 957, 1596, 1823, 2363, 2576
Eco72I CACGTG 2 cut(s) 2168, 2666
Eco81I CCTNAGG 1 cut(s) 201
EcoO109I RGGNCCY 1 cut(s) 2777
EcoRI GAATTC 2 cut(s) 819, 2477
EcoRII CCWGG 2 cut(s) 1743, 2935
EcoRV GATATC 2 cut(s) 1258, 1938
EcoT14I CCWWGG 4 cut(s) 483, 897, 2359, 3137
EcoT22I ATGCAT 3 cut(s) 1419, 1522, 2794
EcoT38I GRGCYC 1 cut(s) 612
ErhI CCWWGG 4 cut(s) 483, 897, 2359, 3137
FalI AAGNNNNNCTT 2 cut(s) 660, 692
FaqI GGGAC 1 cut(s) 806
FauI CCCGC 1 cut(s) 135
FauNDI CATATG 2 cut(s) 1522, 2768
FbaI TGATCA 4 cut(s) 1053, 1275, 1353, 2925
FblI GTMKAC 1 cut(s) 2749
Fnu4HI GCNGC 4 cut(s) 729, 836, 1110, 1314
FriOI GRGCYC 1 cut(s) 612
Fsp4HI GCNGC 4 cut(s) 729, 836, 1110, 1314
GlaI GCGC 2 cut(s) 504, 1344
GluI GCNGC 4 cut(s) 729, 836, 1110, 1314
GsuI CTGGAG 4 cut(s) 291, 527, 1583, 1727
HaeIII GGCC 6 cut(s) 692, 979, 1038, 1439, 1510, 2173
HapII CCGG 5 cut(s) 305, 1244, 1768, 2268, 3024
HgaI GACGC 1 cut(s) 1115
HhaI GCGC 2 cut(s) 505, 1345
Hin6I GCGC 2 cut(s) 503, 1343
HinP1I GCGC 2 cut(s) 503, 1343
HincII GTYRAC 3 cut(s) 990, 2080, 2824
HindII GTYRAC 3 cut(s) 990, 2080, 2824
HindIII AAGCTT 4 cut(s) 128, 368, 1568, 3013
HpaII CCGG 5 cut(s) 305, 1244, 1768, 2268, 3024
Hpy166II GTNNAC 7 cut(s) 51, 587, 990, 2080, 2316, 2750, 2824
Hpy8I GTNNAC 7 cut(s) 51, 587, 990, 2080, 2316, 2750, 2824
Hpy99I CGWCG 2 cut(s) 417, 1157
HpyAV CCTTC 6 cut(s) 125, 443, 969, 1319, 1520, 1627
HpyCH4III ACNGT 6 cut(s) 397, 1060, 1391, 2950, 2973, 3084
HpyCH4IV ACGT 3 cut(s) 579, 2167, 2665
HpyF10VI GCNNNNNNNGC 4 cut(s) 233, 367, 467, 728
HpySE526I ACGT 3 cut(s) 579, 2167, 2665
HspAI GCGC 2 cut(s) 503, 1343
Kpn2I TCCGGA 2 cut(s) 304, 1243
Ksp22I TGATCA 4 cut(s) 1053, 1275, 1353, 2925
LmnI GCTCC 3 cut(s) 272, 1328, 2194
Lsp1109I GCAGC 4 cut(s) 740, 847, 1121, 1300
LweI GCATC 9 cut(s) 28, 129, 214, 350, 836, 1198, 1300, 1404, 1460
MaeII ACGT 3 cut(s) 579, 2167, 2665
MaeIII GTNAC 7 cut(s) 74, 320, 520, 2287, 2413, 2582, 2831
MfeI CAATTG 4 cut(s) 254, 2128, 2575, 2882
MflI RGATCY 5 cut(s) 765, 915, 1638, 1684, 2995
MhlI GDGCHC 6 cut(s) 421, 589, 612, 1072, 1167, 3080
MlsI TGGCCA 2 cut(s) 1439, 1510
MluNI TGGCCA 2 cut(s) 1439, 1510
MlyI GAGTC 8 cut(s) 46, 113, 501, 913, 995, 1735, 2912, 3132
MmeI TCCRAC 5 cut(s) 1071, 1398, 2011, 2667, 3113
Mox20I TGGCCA 2 cut(s) 1439, 1510
Mph1103I ATGCAT 3 cut(s) 1419, 1522, 2794
MroI TCCGGA 2 cut(s) 304, 1243
MscI TGGCCA 2 cut(s) 1439, 1510
MslI CAYNNNNRTG 4 cut(s) 582, 592, 603, 2345
Msp20I TGGCCA 2 cut(s) 1439, 1510
MspA1I CMGCKG 1 cut(s) 2829
MspI CCGG 5 cut(s) 305, 1244, 1768, 2268, 3024
MspR9I CCNGG 3 cut(s) 1745, 2268, 2937
MunI CAATTG 4 cut(s) 254, 2128, 2575, 2882
Mva1269I GAATGC 4 cut(s) 1345, 1409, 1890, 2792
MvaI CCWGG 2 cut(s) 1745, 2937
MvnI CGCG 1 cut(s) 503
MwoI GCNNNNNNNGC 4 cut(s) 233, 367, 467, 728
NciI CCSGG 1 cut(s) 2268
NcoI CCATGG 3 cut(s) 483, 897, 2359
NdeI CATATG 2 cut(s) 1522, 2768
NlaIV GGNNCC 4 cut(s) 853, 1330, 1909, 2442
NmuCI GTSAC 2 cut(s) 520, 2831
NsiI ATGCAT 3 cut(s) 1419, 1522, 2794
NspI RCATGY 2 cut(s) 971, 1972
OliI CACNNNNGTG 2 cut(s) 582, 592
PaeI GCATGC 1 cut(s) 971
PagI TCATGA 1 cut(s) 495
PaqCI CACCTGC 1 cut(s) 266
PceI AGGCCT 1 cut(s) 979
PctI GAATGC 4 cut(s) 1345, 1409, 1890, 2792
PfeI GAWTC 7 cut(s) 158, 192, 944, 1492, 2225, 2470, 2593
PflFI GACNNNGTC 1 cut(s) 518
PkrI GCNGC 4 cut(s) 730, 837, 1111, 1315
PleI GAGTC 8 cut(s) 46, 112, 500, 912, 994, 1734, 2911, 3131
PmaCI CACGTG 2 cut(s) 2168, 2666
PmlI CACGTG 2 cut(s) 2168, 2666
PpsI GAGTC 8 cut(s) 46, 112, 500, 912, 994, 1734, 2911, 3131
Ppu21I YACGTR 2 cut(s) 2168, 2666
PpuMI RGGWCCY 1 cut(s) 2777
PsiI TTATAA 1 cut(s) 2217
Psp5II RGGWCCY 1 cut(s) 2777
Psp6I CCWGG 2 cut(s) 1743, 2935
PspCI CACGTG 2 cut(s) 2168, 2666
PspGI CCWGG 2 cut(s) 1743, 2935
PspN4I GGNNCC 4 cut(s) 853, 1330, 1909, 2442
PspPI GGNCC 3 cut(s) 1908, 2171, 2777
PspPPI RGGWCCY 1 cut(s) 2777
PstI CTGCAG 1 cut(s) 1858
PstNI CAGNNNCTG 2 cut(s) 1695, 2758
PsuI RGATCY 5 cut(s) 765, 915, 1638, 1684, 2995
PsyI GACNNNGTC 1 cut(s) 518
PvuII CAGCTG 1 cut(s) 2829
RsaI GTAC 1 cut(s) 2315
RsaNI GTAC 1 cut(s) 2314
RseI CAYNNNNRTG 4 cut(s) 582, 592, 603, 2345
SatI GCNGC 4 cut(s) 729, 836, 1110, 1314
Sau96I GGNCC 3 cut(s) 1908, 2171, 2777
SchI GAGTC 8 cut(s) 46, 113, 501, 913, 995, 1735, 2912, 3132
ScrFI CCNGG 3 cut(s) 1745, 2268, 2937
SduI GDGCHC 6 cut(s) 421, 589, 612, 1072, 1167, 3080
SfaNI GCATC 9 cut(s) 28, 129, 214, 350, 836, 1198, 1300, 1404, 1460
SfcI CTRYAG 2 cut(s) 1854, 2750
SinI GGWCC 2 cut(s) 1908, 2777
SmiMI CAYNNNNRTG 4 cut(s) 582, 592, 603, 2345
SmlI CTYRAG 1 cut(s) 131
SmoI CTYRAG 1 cut(s) 131
SphI GCATGC 1 cut(s) 971
SseBI AGGCCT 1 cut(s) 979
SsiI CCGC 4 cut(s) 5, 142, 849, 2669
SspI AATATT 3 cut(s) 2068, 2212, 2806
StuI AGGCCT 1 cut(s) 979
StyD4I CCNGG 3 cut(s) 1743, 2266, 2935
StyI CCWWGG 4 cut(s) 483, 897, 2359, 3137
TaaI ACNGT 6 cut(s) 397, 1060, 1391, 2950, 2973, 3084
TaiI ACGT 3 cut(s) 582, 2170, 2668
TaqI TCGA 6 cut(s) 344, 412, 1266, 1706, 2809, 3039
TaqII GACCGA 1 cut(s) 2673
TfiI GAWTC 7 cut(s) 158, 192, 944, 1492, 2225, 2470, 2593
TscAI CASTG 2 cut(s) 451, 2955
TseFI GTSAC 2 cut(s) 520, 2831
TseI GCWGC 4 cut(s) 728, 835, 1109, 1313
Tsp45I GTSAC 2 cut(s) 520, 2831
TspDTI ATGAA 8 cut(s) 44, 74, 512, 720, 1539, 1646, 1914, 2469
TspGWI ACGGA 3 cut(s) 1170, 3036, 3122
TspRI CASTG 2 cut(s) 451, 2955
Tth111I GACNNNGTC 1 cut(s) 518
VneI GTGCAC 1 cut(s) 585
VpaK11BI GGWCC 2 cut(s) 1908, 2777
XbaI TCTAGA 1 cut(s) 2473
XceI RCATGY 2 cut(s) 971, 1972
XcmI CCANNNNNNNNNTGG 2 cut(s) 836, 1381
XmiI GTMKAC 1 cut(s) 2749
Zsp2I ATGCAT 3 cut(s) 1419, 1522, 2794
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.