Rw0G022790

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig01057
OG0000085
Physical Location & Seq
Reverse (-)
15653 .. 19736
4084 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G022790.1

Sequence Viewer

Length: 3675 bp
ATGGAGTTCTATCAGTACGTCTTTGAGGCGATGAGGATGATTATTGTGATTAGTGCGGTAACGGTGCAATGGTTCGGTGTCACAGACAGTCTTAAGTGGATATGGAAGAATTGGTTCATTATTGTGGTGATTGTGACATGGAACGTAATAGCCACTCTTGGGTGGATATGGAAGCATAGGTTCAGTTACATGGTGGAAACCCCAGAGCCAATATCAGAGGATAATCAAGAACCGGTTGACGTCGTTGAAACCCCAGAGCCAATAGCAGAGGGTAATCAAGAACCGGTTGACGTCGTTGAAACCCCAGAGCCAATAGCAGAGGGTAATCAAGAACCGGTTGACGTCGATGCCTCTACATCATCAGCTCCTAGGTGGAAGCACGATGTGTTTTTGAGTTTCAGGGGTGTAGACGCTCGCAAAGGTATTGCATCCGAAATATACTATCGACTGCAAACCAGGAAAGGAATTAAAATTTTCATGGATGACCCAGACCTTCAAGTAGGGAATGTTATTTCTCCCACTCTCCTAACAGCAATTAAAGAATCAAGGTTTGCAATTATTGTTCTCTCCCCAAACTATGCCTCTTCCACTTGGTGTTTGGAGGAACTTAGATACATTTGTGAATGCATGAAAGAAGACGAGGACAGAATTCTGCCAGTTTTTTATTATGTGAATCCTACTGATGTACGATATCAGAAGAGCAGTTTCGGTGATGCTTTCACTAAGCATGAAAATTCTGGGCGATACGGATCAGAGGAGATGCGGCAGTGGAGAGCTGCTTTAAAAAAAGTGGCAAATTTCTCTGGATGGGACACAAAGAATTATAAAACTGAAAGAGCACTTTTGGATGCCGTTGAGGAAACTGTGTGTGATAAATTACGAGCTACTGAACTTGAGCTTAAAATGTCCATTGGAGGTTTTGAAGTATTTGAAGCAACAAAACAAGCCATTGATCAGGTTATGAATGCGCTGAAAGATAATGAGGCCACTACCATTGGAGTCTACGGAATGGGGGGCGTCGGGAAGACGACAATGGTGAATTATGTTGGTGTACGAGCCCAAGAAAATAGGCTTTTTGATAAAGTGATTTTGGCTGTCGTATCCCAAAACCCCGACTTGATCAAAATTCAACAGACATTTGCAGAAATGCTGGGCTTTGAACTCAAGGAGAAGACAGAAATTGTTAGAGCCCGTGAATTGAAGAAGAAAATAATGAGAGGAAACAGGATCCTCATAATCTTGGATGACATTTGGAAGAGAATAGAGTTTTCAGACATTGGAATTCCGAGCCACAACGATCTTCAAAGATGCAATTCCAAAGTTCTACTGACCACCAGAAAATTGGATGTTTGCCGTTCCATGGATTGCCATGCAAACATACGTCTCAATATCTTATCAGAAGAAGATTCTCGGTGCTTATTTGTGAAGGAAGCGAGGAAGTACCCTCTTGACAAATCATCCAATTTCTATGATGTAGCGAGTGAGGTGGCTAGAGAATGCGCTGGTCTTCCTATTGCTTTGATAGCAGTTGCGAGGGCCCTTCGAGGTGAAGGTTTGGACAGATGGAAAGAAGCTGCTCGACGGCTAAAAGCGTCTCAATCCCCCGTCCTTGAAGCTGAGGAAGATGTGTTCAAATGCATAAAGTTAAGCTATGATTACTTGAAATCTGATGTTTCGAAATCATGCTTCTTGCTTTGCTGCCTGTTCCCAGAAGATTATGATATCCCAATTGAATACTTGCTCAATTATGGAATTGGGAAAGGAATGTTTCAAGATTCCGACATGCTAGACGCCCGCGCCACAACAAATTCAGCGGTGAAGGCCCTTAAAGACTCTAGCTTGCTTTTGGACTCTAGACATGACGTATGTGTAAGGATGCATGATGTCATTCGGGATATGGCAATATTGATATCATTATCTGAAGACGGCCCGCGGTTCTTGGTGAAAGTTGGCTGTGAATTGAAGAATTGGCCAAGGATTGATGCACGTAAGGGCTACTCTGCAATCTCACTAATGAAGAACAAAATTTGCAAGCTACCCGAAGAGTTGGTATGTTCAAATCTCCAGATTTTATTACTGCAAGACAATGCTTCATTAAATGATATCCCTAACTCTTTCTTCCAAAATCAGAAAGAATTAAGAGTCTTGGATCTTAGCCGCACTGGTATTTCATTACTACCCCAATCAATCAGTTTCCTAACCAACCTTCAAGCTTTGTATTTAGATTACTGCAAGAACATAATTGACATTTCTGTAATCGGAAAACTTAACAAGCTTGAGATTCTTAGTATGAGAGGAAATGCTCTGAAAGAATTGCCAAGAGAAATAGGACAGTTGACCAGTCTAAGGATGCTGGATATCACTAGAGCTGATAGATTCCTTTTCACATCCACGAATGGATGTATTGGCACAATTCCATCTAAAGTGATATCAAAGTTGCATAGATTAGAAGAACTGTACATGCAATGTGGATTTTGGGAGTGGGGGAGTAAAATTGATGGAGAAGGAGAAGAAACTAATATTGCCTTTGATGAATTAGTTGGTTTATCATATTTAAGTATTTTGAAAGTTTGGATGTCAGATGCAAATTGCATCCCTGTAAGTGTTGAGGTCGAACCGAATTGGGTTTACTTTGATATTACTATTTGCAGCAACAGTTATTTCACTTCGAACAGTCAATCTGGTCATAATTGTAGATCGTTGAGTTTTAATGGAACTGACACTACCATAGGAATCTGTCCGGACTGGTTTGTCAACGCTGTGGTAAAGAATACTGAGAGGCTACTTTATAGTTACTGCAAAGGGTTAAGTAACATTCTTGTGGAATATGACCGTGGAAGGTTACATGGATTGAAAGTTCTCTCTGTAATTGGTTGCCATGAGAACTTGAAAGAATTGATGAATGCAATAACATGTGTTCTAGATATGCCTGTGTTTGAGAATTTGGAAGAGTTGTATCTGGAATACCTGGATTACTTGAAGCAGTTATGTGTCGGTGAGTTACCACCAGGATCTCTCTGCAGTCTGAAATTATTGAAGGTCCTTAAGTGTCCTATCTTGGGGAATGTACTGTTGCCATCTAAATTGTTGCAGCAACTACCAAATCTGGAAAAACTAATCTGTGAGTCAAGTGGAGTGGAACATGTGTTTGGATGTGAAGGTTTTGAGCCAGATCAAACAAATCTGAGAGAGATGGATTTACGGTATCTAGATGTCGTAACAAGCATATGTAATGGTCCTGCTCCACATGGAATGTTCCAGGCTCTAAAGAAATTGGTCATTCGTAATTGCAACTTCCAGGGAAGCCTCTTCACATTTGATGTAGCTCAGTGTCTTTTTCAATTGGAAGACCTTATTGTATCCGAATGCCCTGTCTTGGAAAGAGTAATTGAAGAAGGTAGGGAAACATTGAACAACAAGAAGACCATTCTTCCAAAATTGAAGAACCTAGCTTTGGAAGATCTTCCAATGCTGTACAAGGGAAGTGCTACTATTGATTTTGAGTGTCCTTCATTGGAAAAACTGAGTCTGTTTGGCTGCCCCCACTTGTCATTTCCATCCTCTGCTTCTGACTACTTCCACAGCAGGAACCAAGTCGTTTTCAATGATGCTCCTCGTCATTCTCTGCTACTCAGCAAACTAGGGTATAAGGGATTCAAAATATCAAGTTGGTAG

Protein Analysis

1224

Amino Acids

138.91

Weight (kDa)

5.4

Isoelectric Point (pI)

38.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 126 - 297 9.3e-47 TIR domain
TIR_2 PF13676 129 - 223 6.8e-16 TIR domain
NB-ARC PF00931 314 - 479 5.1e-30 NB-ARC domain
LRR_14 PF23598 672 - 858 2e-12 Leucine-rich repeat region
LRR_8 PF13855 686 - 744 1.9e-08 Leucine rich repeat
LRR_RPS2 PF23247 982 - 1123 1.1e-19 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1064 - 1187 1.5e-12 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 825
AasI GACNNNNNNGTC 1 cut(s) 2750
AatII GACGTC 3 cut(s) 243, 294, 345
AccI GTMKAC 2 cut(s) 408, 1002
AccII CGCG 2 cut(s) 1797, 1933
AccIII TCCGGA 1 cut(s) 2740
AciI CCGC 7 cut(s) 56, 763, 1795, 1814, 1931, 1933, 2158
AclWI GGATC 5 cut(s) 757, 1222, 1235, 2157, 3019
AcoI YGGCCR 1 cut(s) 1970
AcsI RAATTY 9 cut(s) 471, 648, 733, 796, 1125, 1281, 1807, 2025, 2941
AcuI CTGAAG 1 cut(s) 1941
AcyI GRCGYC 5 cut(s) 240, 291, 342, 1017, 1791
AdeI CACNNNGTG 2 cut(s) 385, 594
AfaI GTAC 7 cut(s) 17, 687, 1053, 1442, 2459, 3069, 3476
AfiI CCNNNNNNNGG 6 cut(s) 159, 1360, 2346, 3059, 3376, 3454
AflII CTTAAG 2 cut(s) 92, 3044
AflIII ACRYGT 2 cut(s) 2912, 3142
AgeI ACCGGT 3 cut(s) 232, 283, 334
AjnI CCWGG 5 cut(s) 455, 2967, 3007, 3258, 3297
AjuI GAANNNNNNNTTGG 4 cut(s) 3132, 3164, 3437, 3469
AloI GAACNNNNNNTCC 4 cut(s) 1613, 1645, 2841, 2873
Alw21I GWGCWC 1 cut(s) 841
Alw26I GTCTC 2 cut(s) 1388, 1599
AlwI GGATC 5 cut(s) 757, 1222, 1235, 2157, 3019
Aor13HI TCCGGA 1 cut(s) 2740
AoxI GGCC 5 cut(s) 984, 1536, 1821, 1927, 1970
ApaI GGGCCC 1 cut(s) 1540
ApeKI GCWGC 6 cut(s) 776, 1574, 1698, 2649, 3091, 3537
ApoI RAATTY 9 cut(s) 471, 648, 733, 796, 1125, 1281, 1807, 2025, 2941
ArsI GACNNNNNNTTYG 2 cut(s) 3357, 3389
AsiGI ACCGGT 3 cut(s) 232, 283, 334
Asp700I GAANNNNTTC 2 cut(s) 113, 3462
AspA2I CCTAGG 1 cut(s) 368
AspLEI GCGC 3 cut(s) 970, 1502, 1799
AspS9I GGNCC 6 cut(s) 1536, 1537, 1822, 1928, 3040, 3236
AsuHPI GGTGA 7 cut(s) 139, 722, 1048, 1559, 1828, 1954, 3008
AsuII TTCGAA 2 cut(s) 1676, 2669
AvaII GGWCC 2 cut(s) 3040, 3236
AvrII CCTAGG 1 cut(s) 368
BaeGI GKGCMC 1 cut(s) 1540
BaeI ACNNNNGTAYC 2 cut(s) 604, 637
BalI TGGCCA 1 cut(s) 1972
BamHI GGATCC 1 cut(s) 1227
BanII GRGCYC 3 cut(s) 1060, 1192, 1540
BbsI GAAGAC 7 cut(s) 642, 1031, 1178, 1499, 1929, 3354, 3428
Bbv12I GWGCWC 1 cut(s) 841
BbvCI CCTCAGC 1 cut(s) 1617
BbvI GCAGC 6 cut(s) 763, 1561, 1685, 2661, 3103, 3524
BccI CCATC 7 cut(s) 801, 1557, 2425, 2492, 3085, 3187, 3565
BceAI ACGGC 4 cut(s) 836, 1338, 1598, 1942
BciT130I CCWGG 5 cut(s) 457, 2969, 3009, 3260, 3299
BciVI GTATCC 2 cut(s) 1111, 3370
BclI TGATCA 2 cut(s) 952, 1119
BcoDI GTCTC 2 cut(s) 1388, 1599
BfmI CTRYAG 1 cut(s) 3019
BfrI CTTAAG 2 cut(s) 92, 3044
BfuI GTATCC 2 cut(s) 1111, 3370
BglII AGATCT 1 cut(s) 3460
BisI GCNGC 8 cut(s) 764, 777, 1575, 1699, 2158, 2650, 3092, 3538
BlnI CCTAGG 1 cut(s) 368
BlsI GCNGC 8 cut(s) 765, 778, 1576, 1700, 2159, 2651, 3093, 3539
Bme1390I CCNGG 5 cut(s) 457, 2969, 3009, 3260, 3299
Bme18I GGWCC 2 cut(s) 3040, 3236
BmgT120I GGNCC 6 cut(s) 1536, 1537, 1822, 1928, 3040, 3236
BmiI GGNNCC 3 cut(s) 1229, 1538, 3590
BmrFI CCNGG 5 cut(s) 457, 2969, 3009, 3260, 3299
BpiI GAAGAC 7 cut(s) 642, 1031, 1178, 1499, 1929, 3354, 3428
BpmI CTGGAG 1 cut(s) 2048
Bpu10I CCTNAGC 1 cut(s) 1617
Bpu14I TTCGAA 2 cut(s) 1676, 2669
BpuEI CTTGAG 3 cut(s) 914, 1148, 2297
BsaAI YACGTR 1 cut(s) 1988
BsaBI GATNNNNATC 1 cut(s) 748
BsaHI GRCGYC 5 cut(s) 240, 291, 342, 1017, 1791
BsaJI CCNNGG 6 cut(s) 368, 1359, 1931, 1973, 2833, 3298
BsaWI WCCGGW 4 cut(s) 232, 283, 334, 2740
Bsc4I CCNNNNNNNGG 6 cut(s) 159, 1360, 2346, 3059, 3376, 3454
Bse118I RCCGGY 3 cut(s) 232, 283, 334
Bse1I ACTGG 4 cut(s) 656, 2167, 2340, 2750
Bse3DI GCAATG 2 cut(s) 74, 2471
Bse8I GATNNNNATC 1 cut(s) 748
BseAI TCCGGA 1 cut(s) 2740
BseBI CCWGG 5 cut(s) 457, 2969, 3009, 3260, 3299
BseDI CCNNGG 6 cut(s) 368, 1359, 1931, 1973, 2833, 3298
BseJI GATNNNNATC 1 cut(s) 748
BseLI CCNNNNNNNGG 6 cut(s) 159, 1360, 2346, 3059, 3376, 3454
BseMI GCAATG 2 cut(s) 74, 2471
BseMII CTCAG 6 cut(s) 1608, 2766, 3176, 3341, 3515, 3646
BseNI ACTGG 4 cut(s) 656, 2167, 2340, 2750
BseRI GAGGAG 2 cut(s) 770, 3603
BseSI GKGCMC 1 cut(s) 1540
BseXI GCAGC 6 cut(s) 763, 1561, 1685, 2661, 3103, 3524
BseYI CCCAGC 1 cut(s) 1150
Bsh1236I CGCG 2 cut(s) 1797, 1933
BshFI GGCC 5 cut(s) 986, 1538, 1823, 1929, 1972
BshTI ACCGGT 3 cut(s) 232, 283, 334
BsiHKAI GWGCWC 1 cut(s) 841
BsiSI CCGG 4 cut(s) 233, 284, 335, 2741
BslFI GGGAC 1 cut(s) 824
BslI CCNNNNNNNGG 6 cut(s) 159, 1360, 2346, 3059, 3376, 3454
BsmAI GTCTC 2 cut(s) 1388, 1599
BsmBI CGTCTC 2 cut(s) 1388, 1599
BsmFI GGGAC 1 cut(s) 824
BsmI GAATGC 5 cut(s) 629, 970, 1502, 2908, 3371
BsnI GGCC 5 cut(s) 986, 1538, 1823, 1929, 1972
Bsp119I TTCGAA 2 cut(s) 1676, 2669
Bsp120I GGGCCC 1 cut(s) 1536
Bsp1286I GDGCHC 4 cut(s) 841, 1060, 1192, 1540
Bsp13I TCCGGA 1 cut(s) 2740
Bsp1407I TGTACA 2 cut(s) 2457, 3474
Bsp19I CCATGG 1 cut(s) 1359
BspACI CCGC 7 cut(s) 56, 763, 1795, 1814, 1931, 1933, 2158
BspANI GGCC 5 cut(s) 986, 1538, 1823, 1929, 1972
BspCNI CTCAG 6 cut(s) 1609, 2767, 3177, 3340, 3516, 3645
BspEI TCCGGA 1 cut(s) 2740
BspFNI CGCG 2 cut(s) 1797, 1933
BspLI GGNNCC 3 cut(s) 1229, 1538, 3590
BspMAI CTGCAG 1 cut(s) 3023
BspPI GGATC 5 cut(s) 757, 1222, 1235, 2157, 3019
BspQI GCTCTTC 1 cut(s) 692
BspT104I TTCGAA 2 cut(s) 1676, 2669
BspTI CTTAAG 2 cut(s) 92, 3044
BsrDI GCAATG 2 cut(s) 74, 2471
BsrFI RCCGGY 3 cut(s) 232, 283, 334
BsrGI TGTACA 2 cut(s) 2457, 3474
BsrI ACTGG 4 cut(s) 656, 2167, 2340, 2750
BssAI RCCGGY 3 cut(s) 232, 283, 334
BssECI CCNNGG 6 cut(s) 368, 1359, 1931, 1973, 2833, 3298
BssNI GRCGYC 5 cut(s) 240, 291, 342, 1017, 1791
BssT1I CCWWGG 3 cut(s) 368, 1359, 1973
Bst2UI CCWGG 5 cut(s) 457, 2969, 3009, 3260, 3299
Bst6I CTCTTC 6 cut(s) 589, 692, 1250, 2037, 2943, 3314
BstACI GRCGYC 5 cut(s) 240, 291, 342, 1017, 1791
BstAFI CTTAAG 2 cut(s) 92, 3044
BstAUI TGTACA 2 cut(s) 2457, 3474
BstBAI YACGTR 1 cut(s) 1988
BstBI TTCGAA 2 cut(s) 1676, 2669
BstC8I GCNNGC 5 cut(s) 415, 1795, 1841, 1931, 2033
BstDSI CCRYGG 3 cut(s) 1359, 1931, 2833
BstFNI CGCG 2 cut(s) 1797, 1933
BstHHI GCGC 3 cut(s) 970, 1502, 1799
BstMAI GTCTC 2 cut(s) 1388, 1599
BstMWI GCNNNNNNNGC 2 cut(s) 1523, 1820
BstNI CCWGG 5 cut(s) 457, 2969, 3009, 3260, 3299
BstNSI RCATGY 4 cut(s) 1786, 2464, 2916, 3146
BstSCI CCNGG 5 cut(s) 455, 2967, 3007, 3258, 3297
BstSFI CTRYAG 1 cut(s) 3019
BstSLI GKGCMC 1 cut(s) 1540
BstUI CGCG 2 cut(s) 1797, 1933
BstV1I GCAGC 6 cut(s) 763, 1561, 1685, 2661, 3103, 3524
BstV2I GAAGAC 7 cut(s) 642, 1031, 1178, 1499, 1929, 3354, 3428
BstX2I RGATCY 4 cut(s) 1227, 2149, 3011, 3460
BstXI CCANNNNNNTGG 1 cut(s) 1342
BstYI RGATCY 4 cut(s) 1227, 2149, 3011, 3460
BsuI GTATCC 2 cut(s) 1111, 3370
BsuRI GGCC 5 cut(s) 986, 1538, 1823, 1929, 1972
BtgI CCRYGG 3 cut(s) 1359, 1931, 2833
BtgZI GCGATG 1 cut(s) 44
BtsI GCAGTG 1 cut(s) 773
BtsIMutI CAGTG 3 cut(s) 773, 2160, 3335
Cac8I GCNNGC 5 cut(s) 415, 1795, 1841, 1931, 2033
CfoI GCGC 3 cut(s) 970, 1502, 1799
Cfr10I RCCGGY 3 cut(s) 232, 283, 334
Cfr13I GGNCC 6 cut(s) 1536, 1537, 1822, 1928, 3040, 3236
Cfr42I CCGCGG 1 cut(s) 1934
CseI GACGC 4 cut(s) 419, 1006, 1581, 1799
Csp6I GTAC 7 cut(s) 16, 686, 1052, 1441, 2458, 3068, 3475
CspAI ACCGGT 3 cut(s) 232, 283, 334
CspCI CAANNNNNGTGG 4 cut(s) 976, 1011, 3117, 3152
CviQI GTAC 7 cut(s) 16, 686, 1052, 1441, 2458, 3068, 3475
DraI TTTAAA 1 cut(s) 783
DraIII CACNNNGTG 2 cut(s) 385, 594
DrdI GACNNNNNNGTC 1 cut(s) 2750
DseDI GACNNNNNNGTC 1 cut(s) 2750
EaeI YGGCCR 1 cut(s) 1970
Eam1104I CTCTTC 6 cut(s) 589, 692, 1250, 2037, 2943, 3314
EarI CTCTTC 6 cut(s) 589, 692, 1250, 2037, 2943, 3314
Eco130I CCWWGG 3 cut(s) 368, 1359, 1973
Eco24I GRGCYC 3 cut(s) 1060, 1192, 1540
Eco32I GATATC 6 cut(s) 692, 1723, 1911, 2104, 2359, 2430
Eco47I GGWCC 2 cut(s) 3040, 3236
Eco57I CTGAAG 1 cut(s) 1941
EcoO109I RGGNCCY 4 cut(s) 1536, 1537, 1822, 3040
EcoRI GAATTC 2 cut(s) 648, 1281
EcoRII CCWGG 5 cut(s) 455, 2967, 3007, 3258, 3297
EcoRV GATATC 6 cut(s) 692, 1723, 1911, 2104, 2359, 2430
EcoT14I CCWWGG 3 cut(s) 368, 1359, 1973
EcoT22I ATGCAT 3 cut(s) 629, 1640, 1881
EcoT38I GRGCYC 3 cut(s) 1060, 1192, 1540
ErhI CCWWGG 3 cut(s) 368, 1359, 1973
Esp3I CGTCTC 2 cut(s) 1388, 1599
FaqI GGGAC 1 cut(s) 824
FauI CCCGC 2 cut(s) 1802, 1938
FauNDI CATATG 1 cut(s) 3227
FbaI TGATCA 2 cut(s) 952, 1119
FblI GTMKAC 2 cut(s) 408, 1002
Fnu4HI GCNGC 8 cut(s) 764, 777, 1575, 1699, 2158, 2650, 3092, 3538
FriOI GRGCYC 3 cut(s) 1060, 1192, 1540
Fsp4HI GCNGC 8 cut(s) 764, 777, 1575, 1699, 2158, 2650, 3092, 3538
GlaI GCGC 3 cut(s) 969, 1501, 1798
GluI GCNGC 8 cut(s) 764, 777, 1575, 1699, 2158, 2650, 3092, 3538
GsaI CCCAGC 1 cut(s) 1154
GsuI CTGGAG 1 cut(s) 2048
HaeIII GGCC 5 cut(s) 986, 1538, 1823, 1929, 1972
HapII CCGG 4 cut(s) 233, 284, 335, 2741
HgaI GACGC 4 cut(s) 419, 1006, 1581, 1799
HhaI GCGC 3 cut(s) 970, 1502, 1799
Hin1I GRCGYC 5 cut(s) 240, 291, 342, 1017, 1791
Hin6I GCGC 3 cut(s) 968, 1500, 1797
HinP1I GCGC 3 cut(s) 968, 1500, 1797
HincII GTYRAC 5 cut(s) 238, 289, 340, 2337, 2755
HindII GTYRAC 5 cut(s) 238, 289, 340, 2337, 2755
HindIII AAGCTT 2 cut(s) 2211, 2273
HpaII CCGG 4 cut(s) 233, 284, 335, 2741
HphI GGTGA 7 cut(s) 139, 722, 1048, 1559, 1828, 1954, 3008
Hpy166II GTNNAC 9 cut(s) 238, 289, 340, 409, 1003, 1052, 2337, 2629, 2755
Hpy8I GTNNAC 9 cut(s) 238, 289, 340, 409, 1003, 1052, 2337, 2629, 2755
Hpy99I CGWCG 5 cut(s) 245, 296, 347, 1022, 1584
HpyCH4IV ACGT 8 cut(s) 18, 144, 240, 291, 342, 1381, 1863, 1987
HpyF10VI GCNNNNNNNGC 2 cut(s) 1523, 1820
HpySE526I ACGT 8 cut(s) 18, 144, 240, 291, 342, 1381, 1863, 1987
Hsp92I GRCGYC 5 cut(s) 240, 291, 342, 1017, 1791
HspAI GCGC 3 cut(s) 968, 1500, 1797
Kpn2I TCCGGA 1 cut(s) 2740
Ksp22I TGATCA 2 cut(s) 952, 1119
KspI CCGCGG 1 cut(s) 1934
LguI GCTCTTC 1 cut(s) 692
LmnI GCTCC 3 cut(s) 370, 3247, 3616
Lsp1109I GCAGC 6 cut(s) 763, 1561, 1685, 2661, 3103, 3524
MaeII ACGT 8 cut(s) 18, 144, 240, 291, 342, 1381, 1863, 1987
MaeIII GTNAC 9 cut(s) 58, 79, 133, 185, 2792, 2810, 2841, 3000, 3217
MfeI CAATTG 2 cut(s) 1728, 3341
MflI RGATCY 4 cut(s) 1227, 2149, 3011, 3460
MhlI GDGCHC 4 cut(s) 841, 1060, 1192, 1540
MlsI TGGCCA 1 cut(s) 1972
MluNI TGGCCA 1 cut(s) 1972
MlyI GAGTC 6 cut(s) 1008, 1826, 1844, 2151, 3134, 3535
MmeI TCCRAC 1 cut(s) 1803
Mox20I TGGCCA 1 cut(s) 1972
Mph1103I ATGCAT 3 cut(s) 629, 1640, 1881
MroI TCCGGA 1 cut(s) 2740
MroXI GAANNNNTTC 2 cut(s) 113, 3462
MscI TGGCCA 1 cut(s) 1972
MslI CAYNNNNRTG 2 cut(s) 122, 2819
Msp20I TGGCCA 1 cut(s) 1972
MspA1I CMGCKG 2 cut(s) 1814, 1933
MspCI CTTAAG 2 cut(s) 92, 3044
MspI CCGG 4 cut(s) 233, 284, 335, 2741
MspR9I CCNGG 5 cut(s) 457, 2969, 3009, 3260, 3299
MunI CAATTG 2 cut(s) 1728, 3341
Mva1269I GAATGC 5 cut(s) 629, 970, 1502, 2908, 3371
MvaI CCWGG 5 cut(s) 457, 2969, 3009, 3260, 3299
MvnI CGCG 2 cut(s) 1797, 1933
MwoI GCNNNNNNNGC 2 cut(s) 1523, 1820
NcoI CCATGG 1 cut(s) 1359
NdeI CATATG 1 cut(s) 3227
NlaIV GGNNCC 3 cut(s) 1229, 1538, 3590
NmuCI GTSAC 2 cut(s) 79, 133
NsiI ATGCAT 3 cut(s) 629, 1640, 1881
NspI RCATGY 4 cut(s) 1786, 2464, 2916, 3146
NspV TTCGAA 2 cut(s) 1676, 2669
PciI ACATGT 2 cut(s) 2912, 3142
PciSI GCTCTTC 1 cut(s) 692
PctI GAATGC 5 cut(s) 629, 970, 1502, 2908, 3371
PdmI GAANNNNTTC 2 cut(s) 113, 3462
PfeI GAWTC 8 cut(s) 542, 673, 1406, 1775, 2281, 2376, 2733, 3654
PinAI ACCGGT 3 cut(s) 232, 283, 334
PkrI GCNGC 8 cut(s) 765, 778, 1576, 1700, 2159, 2651, 3093, 3539
PleI GAGTC 6 cut(s) 1007, 1826, 1844, 2150, 3133, 3534
PpsI GAGTC 6 cut(s) 1007, 1826, 1844, 2150, 3133, 3534
Ppu21I YACGTR 1 cut(s) 1988
PpuMI RGGWCCY 1 cut(s) 3040
PscI ACATGT 2 cut(s) 2912, 3142
PsiI TTATAA 1 cut(s) 825
Psp5II RGGWCCY 1 cut(s) 3040
Psp6I CCWGG 5 cut(s) 455, 2967, 3007, 3258, 3297
PspFI CCCAGC 1 cut(s) 1150
PspGI CCWGG 5 cut(s) 455, 2967, 3007, 3258, 3297
PspN4I GGNNCC 3 cut(s) 1229, 1538, 3590
PspOMI GGGCCC 1 cut(s) 1536
PspPI GGNCC 6 cut(s) 1536, 1537, 1822, 1928, 3040, 3236
PspPPI RGGWCCY 1 cut(s) 3040
PsrI GAACNNNNNNTAC 2 cut(s) 2707, 2739
PstI CTGCAG 1 cut(s) 3023
PsuI RGATCY 4 cut(s) 1227, 2149, 3011, 3460
RsaI GTAC 7 cut(s) 17, 687, 1053, 1442, 2459, 3069, 3476
RsaNI GTAC 7 cut(s) 16, 686, 1052, 1441, 2458, 3068, 3475
RseI CAYNNNNRTG 2 cut(s) 122, 2819
SacII CCGCGG 1 cut(s) 1934
SapI GCTCTTC 1 cut(s) 692
SatI GCNGC 8 cut(s) 764, 777, 1575, 1699, 2158, 2650, 3092, 3538
Sau96I GGNCC 6 cut(s) 1536, 1537, 1822, 1928, 3040, 3236
SchI GAGTC 6 cut(s) 1008, 1826, 1844, 2151, 3134, 3535
ScrFI CCNGG 5 cut(s) 457, 2969, 3009, 3260, 3299
SduI GDGCHC 4 cut(s) 841, 1060, 1192, 1540
SfcI CTRYAG 1 cut(s) 3019
Sfr303I CCGCGG 1 cut(s) 1934
SfuI TTCGAA 2 cut(s) 1676, 2669
SgrBI CCGCGG 1 cut(s) 1934
SinI GGWCC 2 cut(s) 3040, 3236
SmiMI CAYNNNNRTG 2 cut(s) 122, 2819
SmlI CTYRAG 5 cut(s) 92, 893, 1163, 2276, 3044
SmoI CTYRAG 5 cut(s) 92, 893, 1163, 2276, 3044
SsiI CCGC 7 cut(s) 56, 763, 1795, 1814, 1931, 1933, 2158
SspI AATATT 2 cut(s) 1905, 2521
StyD4I CCNGG 5 cut(s) 455, 2967, 3007, 3258, 3297
StyI CCWWGG 3 cut(s) 368, 1359, 1973
TaiI ACGT 8 cut(s) 21, 147, 243, 294, 345, 1384, 1866, 1990
TaqI TCGA 7 cut(s) 345, 445, 1543, 1579, 1676, 2613, 2669
TatI WGTACW 3 cut(s) 2457, 3067, 3474
TauI GCSGC 2 cut(s) 766, 2160
TfiI GAWTC 8 cut(s) 542, 673, 1406, 1775, 2281, 2376, 2733, 3654
TscAI CASTG 3 cut(s) 773, 2167, 3335
TseFI GTSAC 2 cut(s) 79, 133
TseI GCWGC 6 cut(s) 776, 1574, 1698, 2649, 3091, 3537
Tsp45I GTSAC 2 cut(s) 79, 133
TspGWI ACGGA 2 cut(s) 762, 1020
TspRI CASTG 3 cut(s) 773, 2167, 3335
Vha464I CTTAAG 2 cut(s) 92, 3044
VpaK11BI GGWCC 2 cut(s) 3040, 3236
XapI RAATTY 9 cut(s) 471, 648, 733, 796, 1125, 1281, 1807, 2025, 2941
XbaI TCTAGA 3 cut(s) 1853, 2920, 3208
XceI RCATGY 4 cut(s) 1786, 2464, 2916, 3146
XcmI CCANNNNNNNNNTGG 2 cut(s) 595, 1339
XmaJI CCTAGG 1 cut(s) 368
XmiI GTMKAC 2 cut(s) 408, 1002
XmnI GAANNNNTTC 2 cut(s) 113, 3462
ZraI GACGTC 3 cut(s) 241, 292, 343
Zsp2I ATGCAT 3 cut(s) 629, 1640, 1881
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.