FvH4_7g12671

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000085
Physical Location & Seq
Forward (+)
11487291 .. 11491126
3836 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g12671.t1

Sequence Viewer

Length: 3327 bp
ATGGGGCAAACAACCTCCGCATCTATTCCTTCCTCAGCTGACTCTGTATCTCTTACATCGGCAGCTGAATCATCTTCAGCTGCATCTGCATCTTTTTCATCATCATCAACTCCTCAGTCGAGTTATGACGTGTTTTTGAGTTTCAGGGGTCCTGACACTCGACAGGGTATTGTTTTTGAATTATATGACCGATTGAATAATACGAGAGGAGTTAAAACATTCAAGGATGACTCAGACCTTGAAGTAGGAGATGCCATTTCTCCCACTCTCCTAGCGGCAATCGAACAATCAAGGTTTGCTATTGTTGTTCTCTCAGAAAACTATGCTGCTTCCACTTGGTGTTTGGAGGAACTTGCAAAGATTTGTCAATGCATGCAAGACAATAGAATTCTTCCACTTTTTTATCATGTCGATCCTACTGATGTACGATATCAGAAGAAGAGCTTTGAAACTGCTTTTAATAGGCATGAAAAATCCAGGCGACATAAAACAGAGAAGGTGAAGCAGTGGAGAGATGCTTTGAACAAAGTGGCCAATTTCTCTGGGTGGCATACACAAAATTATAAGGCTGAGAGAGAACTTGTAGACGCCATTGTGGAATTTGTCTGCAGTAAAGTACTGCCTGCTTCAATTCAGTCCACAGGAGATTTTCATGCCTTTGAAGCAACAAGACAAGCCATGGATAAGGTTATGAAGGCGCTAAAAGATGACAAGATCACTGTCATTGCTGTTCATGGCATAGGAGGCGTCGGAAAGACGACCTTGGTGGAACATGCTGGTCAAGAAGCTCATAATCAACGCCTTTTTAATCATGTGATAAAGATTGTGGTGACTCAAACCCTTGACTTGAGAAAAATTCAAGGAACATTGGCAGATGGTCTAGGCATCACATTGACGCAAGATTCAGAAGATGGAAGAGCTTCTAGATTGAATAAGGAAATAATGAGAAAAAACAAGCTCCTCATAATCGTGGACGACGTTTGGGAGAGGGTAGAACTGGCAAGCATAGGGGTTCCCAACTATGACATGCTTCAGAGTTGCAGCTCCAAAGTCATATTCACCACAAGGATACGGAACGTATGTCATGTCATGGGTAGCCACGAAAAGATCACCCTCAGTGTTCTGTCAGAAGAAGATTCATGGGCCTTGTTTGTGAAAAATGCAGGAAGATCATTTGAACCGACCAATTTTGAGGATGTAGCCAAGAAGGTAGCGAGAGAGTGTAAGGGTCTACCAATTGCATTGATAGCAGTTGCAAGGGCACTTGGAGACAAAGAACTGGTGGAATGGAAAACAGCAGCCCGACGACTAGAGAAGTCGCAAAATGCCAACCCAGACGACAAGGGAGTTGCTTCCGAAAGTGTAAAATTAAGTTATGATTACTTGAAAGATGAGGAGTGCAAGTCATGTTTCTTGCTCTGCTGCTTATTCCCAGAAGACAGTAGCATCGAAATAGAACAGTTGTTCAGGTATGCCATAGGGAAAGGATTGTTTCGAGATGCCAACACAATCGAAGAAGCCAGAGGTACCGCAAATTCAGTAGTCAACTACTTGAAAAATTCTAGCTTGCTTATGGATACTGGACACCATCAATTTGTAAAGATGCATGATGTCATCCGGGATACAGCCATTCAAATTGCGCAATCTGTAGATGGATTTTTGGTGAAAGCAGGCTATGGTTTGAGGGATTGGCCTCGATTACATGAAGACTGCTCTGCAATCTCACTAATGGAGAATTATATTCGCAAGCTACCCGGACGGTTAGTAATTCCAAAACTCCAGATTTTGTTACTAGAAGATAGTAGCTTCCTAGAAGAGATCCCGGGAACTTTTTTTCAAAGTTTGGAAGAATTAAGGGTCTTGGATCTTAGTCGGACTAGAATTTCATTACTACCCCAGTCGTTGAGTCTGTTAACCAACCTCCAAGTTTTCCTTTTAAATAATTGTCGGCACATAATTGATATTTCAATTGTCGGAAAACTTGAGAAGCTTGAGATTCTTAGTATGAGAAGGTATCCTATGAATGAGTTATTGAGAGAAATAGGAGATTTGACCAGTCTAAGGATGTTGGATTTGACTGGCGGACACATTTCCACAATTCCATCAGGAGTGCTATCAAAGTTGAGTAACTTAGAAGAACTGTACATTCCAGATGGATTTCAGGAATGGGGGAGTAAAATTGAGGGAGAAGAAGAAGAAACCAATGCTAGCTTTGAAGAGGTAACTAGCTTGTCAAATTTAAGAGTTTTGGAGGCTCGGTTATCCAGTGTAGATTGTCTCCCCAAAAATGAAAAACTCAATCCAAATTGGGTTGAGTTTAGTCTACTTATCGGCAGCATGATTAGGAAATCGAATATTGGTGCGCATGATCATAATTCAATAATCTTGTCCCTTGATGGAACCATGAGCACCTTACCGAGGTGGTTTTTGAACGTGGTGATGAACGGAGCAGATAAGCTAGAGTTGTTCTGGTGCCAAGGGCTAATTGACATTTTCTCAGAATATGACCATGGGAGGTTACATAGACTCAAGTATCTCTCTGTAAGGGGTTCGCCAGAGATGATGAACACAACAGTGACAACACCATGGATTCCAAAAGAACCTGTGTTCAAGAACCTAGAAGAGTTGCATCTGGATTATCTGGATTGCGGTTTTTGTGTTACTAAATTACTACCGGGGTCTCTGTTCAATCTAAAGTTGCTGAAGATAGCACGTTGTTCTAACTGGGGGAATGTACTTTTACCATCAAAATTGTTACATAGACTACCAAATCTGGAAAAACTATTTTGTTACGATGTGCCTGAAATGTTATATGTGCTTGGATGTGAAGGGTTATTTGAGCCAGAACCATCAAAATTGAGTGACCTGCAGTTGTATCGTCTAGCTGCACTAAGAAGCATATGTAATGGACCTGCTCCACCTGCAATGTTCCAAGCTCTTAAAAGTTTGTTACTATTCCGGTGCAATTTGCAGGGAAGTCTCTTCAGCAATGATGCTGCTCAATATCTTTTTCAATTGGAAGACATTTGTATAGTCTTTTGCCCTCTTTTGGAAAGAATAATTGAAGCAAGTACCAAGCAGAACATTCTTCCAAAATTGAAGAACTTGACGTTGTGGAGACTTCCAATGTTGTACTGTGAAAGTGCTATTGATATCGAGTGTCCTTCATTGGTACACCTGTATGTGAAGGACTGCCCTCAAATTTCAGTCTCTTTTTCCGACTTCCGTAGCAGGAAACCATTCCAACTCAATAATGAAATAATTTACAGGTTGAAAGATATCAATCCCCTCAATGACTTTGCTCAACTGGAAGCAAGAAGTTCATGA

Protein Analysis

1109

Amino Acids

124.94

Weight (kDa)

5.93

Isoelectric Point (pI)

41.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 42 - 208 1.3e-48 TIR domain
TIR_2 PF13676 44 - 139 4.2e-14 TIR domain
NB-ARC PF00931 225 - 389 5.9e-31 NB-ARC domain
LRR_8 PF13855 591 - 650 2.9e-07 Leucine rich repeat
LRR_14 PF23598 641 - 757 1.1e-09 Leucine-rich repeat region
LRR_RPS2 PF23247 894 - 1014 2.6e-12 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 564
AarI CACCTGC 1 cut(s) 2929
Acc16I TGCGCA 2 cut(s) 1641, 2364
Acc36I ACCTGC 3 cut(s) 2874, 2920, 2929
Acc65I GGTACC 1 cut(s) 1526
AccB1I GGYRCC 2 cut(s) 1526, 2472
AccI GTMKAC 3 cut(s) 585, 1231, 2323
AciI CCGC 5 cut(s) 18, 275, 1530, 2082, 2649
AclWI GGATC 3 cut(s) 407, 1813, 1872
AcoI YGGCCR 1 cut(s) 531
AcsI RAATTY 8 cut(s) 387, 599, 855, 1534, 1558, 1881, 2236, 3201
AcuI CTGAAG 4 cut(s) 60, 1016, 2723, 2968
AcyI GRCGYC 2 cut(s) 588, 747
AdeI CACNNNGTG 1 cut(s) 339
AfaI GTAC 8 cut(s) 426, 618, 1528, 2144, 2736, 3073, 3134, 3174
AfiI CCNNNNNNNGG 3 cut(s) 2061, 2418, 3049
AflIII ACRYGT 1 cut(s) 129
AjiI CACGTC 1 cut(s) 130
AjnI CCWGG 1 cut(s) 476
AjuI GAANNNNNNNTTGG 2 cut(s) 1040, 1072
AleI CACNNNNGTG 1 cut(s) 2573
Alw21I GWGCWC 1 cut(s) 2411
Alw26I GTCTC 6 cut(s) 1263, 2282, 2685, 2984, 3112, 3214
AlwI GGATC 3 cut(s) 407, 1813, 1872
Ama87I CYCGRG 1 cut(s) 1822
AoxI GGCC 3 cut(s) 531, 1143, 1691
ApeKI GCWGC 9 cut(s) 62, 80, 326, 1041, 1298, 1422, 2334, 2885, 2996
ApoI RAATTY 8 cut(s) 387, 599, 855, 1534, 1558, 1881, 2236, 3201
Asp700I GAANNNNTTC 2 cut(s) 919, 3239
Asp718I GGTACC 1 cut(s) 1526
AspLEI GCGC 3 cut(s) 700, 1642, 2365
AspS9I GGNCC 3 cut(s) 149, 1143, 2909
AsuC2I CCSGG 5 cut(s) 1619, 1755, 1823, 1824, 2676
AsuHPI GGTGA 6 cut(s) 511, 841, 1051, 1102, 1675, 2449
AsuNHI GCTAGC 1 cut(s) 2207
AvaI CYCGRG 1 cut(s) 1822
AvaII GGWCC 2 cut(s) 149, 2909
BaeGI GKGCMC 1 cut(s) 1264
BaeI ACNNNNGTAYC 4 cut(s) 2516, 2549, 3164, 3197
BalI TGGCCA 1 cut(s) 533
BanI GGYRCC 2 cut(s) 1526, 2472
BbsI GAAGAC 3 cut(s) 1443, 1713, 3027
Bbv12I GWGCWC 1 cut(s) 2411
BbvCI CCTCAGC 1 cut(s) 34
BbvI GCAGC 9 cut(s) 67, 74, 313, 1053, 1310, 1409, 2346, 2872, 2983
BccI CCATC 9 cut(s) 869, 905, 1596, 1646, 2110, 2147, 2390, 2752, 2857
BcgI CGANNNNNNTGC 2 cut(s) 2858, 2892
BciT130I CCWGG 1 cut(s) 478
BciVI GTATCC 4 cut(s) 1062, 1570, 1615, 2025
BclI TGATCA 1 cut(s) 2368
BcnI CCSGG 5 cut(s) 1619, 1755, 1823, 1824, 2676
BcoDI GTCTC 6 cut(s) 1263, 2282, 2685, 2984, 3112, 3214
BfmI CTRYAG 3 cut(s) 607, 1647, 2867
BfoI RGCGCY 1 cut(s) 701
BfuAI ACCTGC 3 cut(s) 2874, 2920, 2929
BfuI GTATCC 4 cut(s) 1062, 1570, 1615, 2025
BmcAI AGTACT 1 cut(s) 618
Bme1390I CCNGG 6 cut(s) 478, 1619, 1755, 1823, 1824, 2676
Bme18I GGWCC 2 cut(s) 149, 2909
BmeT110I CYCGRG 1 cut(s) 1822
BmgBI CACGTC 1 cut(s) 130
BmgT120I GGNCC 3 cut(s) 149, 1143, 2909
BmiI GGNNCC 5 cut(s) 150, 1014, 1528, 2401, 2474
BmrFI CCNGG 6 cut(s) 478, 1619, 1755, 1823, 1824, 2676
BmrI ACTGGG 2 cut(s) 1891, 2734
BmtI GCTAGC 1 cut(s) 2211
BmuI ACTGGG 2 cut(s) 1891, 2734
BpiI GAAGAC 3 cut(s) 1443, 1713, 3027
BpmI CTGGAG 1 cut(s) 1763
Bpu10I CCTNAGC 1 cut(s) 34
BpuEI CTTGAG 4 cut(s) 868, 2003, 2012, 2513
BpuMI CCSGG 5 cut(s) 1619, 1755, 1823, 1824, 2676
BsaBI GATNNNNATC 1 cut(s) 3282
BsaHI GRCGYC 2 cut(s) 588, 747
BsaI GGTCTC 1 cut(s) 2685
BsaJI CCNNGG 8 cut(s) 678, 762, 1822, 2417, 2476, 2509, 2585, 2675
BsaWI WCCGGW 1 cut(s) 2958
BsaXI ACNNNNNCTCC 4 cut(s) 1388, 1418, 2243, 2273
Bsc4I CCNNNNNNNGG 3 cut(s) 2061, 2418, 3049
Bse1I ACTGG 9 cut(s) 1002, 1284, 1585, 1897, 2055, 2083, 2265, 2729, 3312
Bse3DI GCAATG 3 cut(s) 723, 2931, 2995
Bse8I GATNNNNATC 1 cut(s) 3282
BseBI CCWGG 1 cut(s) 478
BseDI CCNNGG 8 cut(s) 678, 762, 1822, 2417, 2476, 2509, 2585, 2675
BseGI GGATG 5 cut(s) 232, 1201, 1614, 2070, 2828
BseJI GATNNNNATC 1 cut(s) 3282
BseLI CCNNNNNNNGG 3 cut(s) 2061, 2418, 3049
BseMI GCAATG 3 cut(s) 723, 2931, 2995
BseMII CTCAG 7 cut(s) 48, 128, 246, 327, 561, 1129, 2511
BseNI ACTGG 9 cut(s) 1002, 1284, 1585, 1897, 2055, 2083, 2265, 2729, 3312
BseRI GAGGAG 4 cut(s) 102, 222, 950, 1409
BseSI GKGCMC 1 cut(s) 1264
BseXI GCAGC 9 cut(s) 67, 74, 313, 1053, 1310, 1409, 2346, 2872, 2983
BsgI GTGCAG 1 cut(s) 2871
BshFI GGCC 3 cut(s) 533, 1145, 1693
BshNI GGYRCC 2 cut(s) 1526, 2472
BsiHKAI GWGCWC 1 cut(s) 2411
BsiHKCI CYCGRG 1 cut(s) 1822
BsiSI CCGG 5 cut(s) 1618, 1755, 1823, 2675, 2959
BslFI GGGAC 1 cut(s) 2374
BslI CCNNNNNNNGG 3 cut(s) 2061, 2418, 3049
BsmAI GTCTC 6 cut(s) 1263, 2282, 2685, 2984, 3112, 3214
BsmFI GGGAC 1 cut(s) 2374
BsnI GGCC 3 cut(s) 533, 1145, 1693
Bso31I GGTCTC 1 cut(s) 2685
BsoBI CYCGRG 1 cut(s) 1822
Bsp1286I GDGCHC 2 cut(s) 1264, 2411
Bsp1407I TGTACA 1 cut(s) 2142
Bsp143I GATC 7 cut(s) 412, 714, 1107, 1169, 1818, 1864, 2368
Bsp19I CCATGG 3 cut(s) 678, 2509, 2585
BspACI CCGC 5 cut(s) 18, 275, 1530, 2082, 2649
BspANI GGCC 3 cut(s) 533, 1145, 1693
BspCNI CTCAG 7 cut(s) 47, 127, 245, 326, 562, 1128, 2510
BspHI TCATGA 1 cut(s) 3323
BspLI GGNNCC 5 cut(s) 150, 1014, 1528, 2401, 2474
BspMAI CTGCAG 2 cut(s) 611, 2871
BspMI ACCTGC 3 cut(s) 2874, 2920, 2929
BspOI GCTAGC 1 cut(s) 2211
BspPI GGATC 3 cut(s) 407, 1813, 1872
BspQI GCTCTTC 2 cut(s) 434, 910
BspT107I GGYRCC 2 cut(s) 1526, 2472
BspTNI GGTCTC 1 cut(s) 2685
BsrDI GCAATG 3 cut(s) 723, 2931, 2995
BsrGI TGTACA 1 cut(s) 2142
BsrI ACTGG 9 cut(s) 1002, 1284, 1585, 1897, 2055, 2083, 2265, 2729, 3312
BssECI CCNNGG 8 cut(s) 678, 762, 1822, 2417, 2476, 2509, 2585, 2675
BssMI GATC 7 cut(s) 412, 714, 1107, 1169, 1818, 1864, 2368
BssNI GRCGYC 2 cut(s) 588, 747
BssT1I CCWWGG 5 cut(s) 678, 762, 2476, 2509, 2585
Bst2UI CCWGG 1 cut(s) 478
Bst4CI ACNGT 7 cut(s) 721, 1442, 1461, 1761, 2142, 2575, 3137
Bst6I CTCTTC 6 cut(s) 434, 910, 1809, 2211, 2616, 2987
BstACI GRCGYC 2 cut(s) 588, 747
BstAUI TGTACA 1 cut(s) 2142
BstC8I GCNNGC 7 cut(s) 374, 624, 1003, 1568, 1672, 1748, 2209
BstDSI CCRYGG 3 cut(s) 678, 2509, 2585
BstENI CCTNNNNNAGG 1 cut(s) 2416
BstF5I GGATG 5 cut(s) 232, 1201, 1614, 2070, 2828
BstH2I RGCGCY 1 cut(s) 701
BstHHI GCGC 3 cut(s) 700, 1642, 2365
BstKTI GATC 7 cut(s) 415, 717, 1110, 1172, 1821, 1867, 2371
BstMAI GTCTC 6 cut(s) 1263, 2282, 2685, 2984, 3112, 3214
BstMBI GATC 7 cut(s) 412, 714, 1107, 1169, 1818, 1864, 2368
BstMWI GCNNNNNNNGC 5 cut(s) 86, 662, 744, 1247, 2921
BstNI CCWGG 1 cut(s) 478
BstNSI RCATGY 3 cut(s) 376, 776, 1030
BstSCI CCNGG 6 cut(s) 476, 1617, 1753, 1821, 1822, 2674
BstSFI CTRYAG 3 cut(s) 607, 1647, 2867
BstSLI GKGCMC 1 cut(s) 1264
BstV1I GCAGC 9 cut(s) 67, 74, 313, 1053, 1310, 1409, 2346, 2872, 2983
BstV2I GAAGAC 3 cut(s) 1443, 1713, 3027
BstX2I RGATCY 2 cut(s) 1818, 1864
BstYI RGATCY 2 cut(s) 1818, 1864
BsuI GTATCC 4 cut(s) 1062, 1570, 1615, 2025
BsuRI GGCC 3 cut(s) 533, 1145, 1693
BtgI CCRYGG 3 cut(s) 678, 2509, 2585
BtrI CACGTC 1 cut(s) 130
BtsCI GGATG 5 cut(s) 232, 1201, 1614, 2070, 2828
BtsI GCAGTG 1 cut(s) 512
BtsIMutI CAGTG 5 cut(s) 512, 717, 1123, 2272, 2580
BveI ACCTGC 3 cut(s) 2874, 2920, 2929
Cac8I GCNNGC 7 cut(s) 374, 624, 1003, 1568, 1672, 1748, 2209
CciI TCATGA 1 cut(s) 3323
CfoI GCGC 3 cut(s) 700, 1642, 2365
Cfr13I GGNCC 3 cut(s) 149, 1143, 2909
Cfr9I CCCGGG 1 cut(s) 1822
CseI GACGC 3 cut(s) 596, 736, 904
Csp6I GTAC 8 cut(s) 425, 617, 1527, 2143, 2735, 3072, 3133, 3173
CviQI GTAC 8 cut(s) 425, 617, 1527, 2143, 2735, 3072, 3133, 3173
DpnI GATC 7 cut(s) 414, 716, 1109, 1171, 1820, 1866, 2370
DpnII GATC 7 cut(s) 412, 714, 1107, 1169, 1818, 1864, 2368
DraI TTTAAA 1 cut(s) 1938
DraIII CACNNNGTG 1 cut(s) 339
EaeI YGGCCR 1 cut(s) 531
Eam1104I CTCTTC 6 cut(s) 434, 910, 1809, 2211, 2616, 2987
EarI CTCTTC 6 cut(s) 434, 910, 1809, 2211, 2616, 2987
EciI GGCGGA 1 cut(s) 2097
Eco130I CCWWGG 5 cut(s) 678, 762, 2476, 2509, 2585
Eco31I GGTCTC 1 cut(s) 2685
Eco32I GATATC 3 cut(s) 431, 3154, 3280
Eco47I GGWCC 2 cut(s) 149, 2909
Eco57I CTGAAG 4 cut(s) 60, 1016, 2723, 2968
Eco88I CYCGRG 1 cut(s) 1822
EcoNI CCTNNNNNAGG 1 cut(s) 2416
EcoO109I RGGNCCY 1 cut(s) 149
EcoRI GAATTC 1 cut(s) 387
EcoRII CCWGG 1 cut(s) 476
EcoRV GATATC 3 cut(s) 431, 3154, 3280
EcoT14I CCWWGG 5 cut(s) 678, 762, 2476, 2509, 2585
EcoT22I ATGCAT 2 cut(s) 374, 1608
ErhI CCWWGG 5 cut(s) 678, 762, 2476, 2509, 2585
FalI AAGNNNNNCTT 6 cut(s) 428, 460, 746, 778, 1917, 1949
FaqI GGGAC 1 cut(s) 2374
FauNDI CATATG 1 cut(s) 2900
FbaI TGATCA 1 cut(s) 2368
FblI GTMKAC 3 cut(s) 585, 1231, 2323
FokI GGATG 5 cut(s) 239, 1208, 1601, 2077, 2835
FspAI RTGCGCAY 1 cut(s) 2364
FspI TGCGCA 2 cut(s) 1641, 2364
GlaI GCGC 3 cut(s) 699, 1641, 2364
GsuI CTGGAG 1 cut(s) 1763
HaeII RGCGCY 1 cut(s) 701
HaeIII GGCC 3 cut(s) 533, 1145, 1693
HapII CCGG 5 cut(s) 1618, 1755, 1823, 2675, 2959
HgaI GACGC 3 cut(s) 596, 736, 904
HhaI GCGC 3 cut(s) 700, 1642, 2365
Hin1I GRCGYC 2 cut(s) 588, 747
Hin6I GCGC 3 cut(s) 698, 1640, 2363
HinP1I GCGC 3 cut(s) 698, 1640, 2363
HincII GTYRAC 2 cut(s) 1546, 1914
HindII GTYRAC 2 cut(s) 1546, 1914
HindIII AAGCTT 1 cut(s) 1988
HpaI GTTAAC 1 cut(s) 1914
HpaII CCGG 5 cut(s) 1618, 1755, 1823, 2675, 2959
HphI GGTGA 6 cut(s) 511, 841, 1051, 1102, 1675, 2449
Hpy166II GTNNAC 8 cut(s) 586, 639, 973, 1232, 1546, 1914, 2324, 3175
Hpy8I GTNNAC 8 cut(s) 586, 639, 973, 1232, 1546, 1914, 2324, 3175
Hpy99I CGWCG 3 cut(s) 752, 980, 1308
HpyAV CCTTC 8 cut(s) 39, 490, 688, 1201, 2004, 2822, 3174, 3181
HpyCH4III ACNGT 7 cut(s) 721, 1442, 1461, 1761, 2142, 2575, 3137
HpyCH4IV ACGT 6 cut(s) 129, 978, 1077, 2433, 2713, 3110
HpyF10VI GCNNNNNNNGC 5 cut(s) 86, 662, 744, 1247, 2921
HpySE526I ACGT 6 cut(s) 129, 978, 1077, 2433, 2713, 3110
Hsp92I GRCGYC 2 cut(s) 588, 747
HspAI GCGC 3 cut(s) 698, 1640, 2363
KpnI GGTACC 1 cut(s) 1530
Ksp22I TGATCA 1 cut(s) 2368
KspAI GTTAAC 1 cut(s) 1914
Kzo9I GATC 7 cut(s) 412, 714, 1107, 1169, 1818, 1864, 2368
LguI GCTCTTC 2 cut(s) 434, 910
LmnI GCTCC 4 cut(s) 963, 1049, 2447, 2920
Lsp1109I GCAGC 9 cut(s) 67, 74, 313, 1053, 1310, 1409, 2346, 2872, 2983
MaeII ACGT 6 cut(s) 129, 978, 1077, 2433, 2713, 3110
MalI GATC 7 cut(s) 414, 716, 1109, 1171, 1820, 1866, 2370
MboI GATC 7 cut(s) 412, 714, 1107, 1169, 1818, 1864, 2368
MfeI CAATTG 3 cut(s) 1236, 1968, 3014
MflI RGATCY 2 cut(s) 1818, 1864
MhlI GDGCHC 2 cut(s) 1264, 2411
MlsI TGGCCA 1 cut(s) 533
MluNI TGGCCA 1 cut(s) 533
MlyI GAGTC 5 cut(s) 35, 224, 826, 1915, 2520
MmeI TCCRAC 6 cut(s) 730, 1853, 1954, 2049, 3243, 3268
Mox20I TGGCCA 1 cut(s) 533
Mph1103I ATGCAT 2 cut(s) 374, 1608
MroXI GAANNNNTTC 2 cut(s) 919, 3239
MscI TGGCCA 1 cut(s) 533
MseI TTAA 9 cut(s) 213, 459, 807, 1370, 1853, 1913, 1937, 2240, 2940
MslI CAYNNNNRTG 3 cut(s) 968, 2573, 3180
Msp20I TGGCCA 1 cut(s) 533
MspA1I CMGCKG 3 cut(s) 38, 65, 80
MspI CCGG 5 cut(s) 1618, 1755, 1823, 2675, 2959
MspR9I CCNGG 6 cut(s) 478, 1619, 1755, 1823, 1824, 2676
MunI CAATTG 3 cut(s) 1236, 1968, 3014
MvaI CCWGG 1 cut(s) 478
MwoI GCNNNNNNNGC 5 cut(s) 86, 662, 744, 1247, 2921
NciI CCSGG 5 cut(s) 1619, 1755, 1823, 1824, 2676
NcoI CCATGG 3 cut(s) 678, 2509, 2585
NdeI CATATG 1 cut(s) 2900
NdeII GATC 7 cut(s) 412, 714, 1107, 1169, 1818, 1864, 2368
NheI GCTAGC 1 cut(s) 2207
NlaIV GGNNCC 5 cut(s) 150, 1014, 1528, 2401, 2474
NmuCI GTSAC 3 cut(s) 829, 2575, 2861
NsbI TGCGCA 2 cut(s) 1641, 2364
NsiI ATGCAT 2 cut(s) 374, 1608
NspI RCATGY 3 cut(s) 376, 776, 1030
OliI CACNNNNGTG 1 cut(s) 2573
PaeI GCATGC 1 cut(s) 376
PagI TCATGA 1 cut(s) 3323
PaqCI CACCTGC 1 cut(s) 2929
PciSI GCTCTTC 2 cut(s) 434, 910
PcsI WCGNNNNNNNCGW 1 cut(s) 975
PdmI GAANNNNTTC 2 cut(s) 919, 3239
PfeI GAWTC 5 cut(s) 68, 902, 1136, 1996, 2590
PfoI TCCNGGA 1 cut(s) 1617
PleI GAGTC 5 cut(s) 35, 224, 826, 1914, 2520
PpsI GAGTC 5 cut(s) 35, 224, 826, 1914, 2520
PpuMI RGGWCCY 1 cut(s) 149
PsiI TTATAA 1 cut(s) 564
Psp5II RGGWCCY 1 cut(s) 149
Psp6I CCWGG 1 cut(s) 476
PspGI CCWGG 1 cut(s) 476
PspN4I GGNNCC 5 cut(s) 150, 1014, 1528, 2401, 2474
PspPI GGNCC 3 cut(s) 149, 1143, 2909
PspPPI RGGWCCY 1 cut(s) 149
PstI CTGCAG 2 cut(s) 611, 2871
PsuI RGATCY 2 cut(s) 1818, 1864
PvuII CAGCTG 3 cut(s) 38, 65, 80
RsaI GTAC 8 cut(s) 426, 618, 1528, 2144, 2736, 3073, 3134, 3174
RsaNI GTAC 8 cut(s) 425, 617, 1527, 2143, 2735, 3072, 3133, 3173
RseI CAYNNNNRTG 3 cut(s) 968, 2573, 3180
SapI GCTCTTC 2 cut(s) 434, 910
SaqAI TTAA 9 cut(s) 213, 459, 807, 1370, 1853, 1913, 1937, 2240, 2940
Sau3AI GATC 7 cut(s) 412, 714, 1107, 1169, 1818, 1864, 2368
Sau96I GGNCC 3 cut(s) 149, 1143, 2909
ScaI AGTACT 1 cut(s) 618
SchI GAGTC 5 cut(s) 35, 224, 826, 1915, 2520
ScrFI CCNGG 6 cut(s) 478, 1619, 1755, 1823, 1824, 2676
SduI GDGCHC 2 cut(s) 1264, 2411
SfcI CTRYAG 3 cut(s) 607, 1647, 2867
SinI GGWCC 2 cut(s) 149, 2909
SmaI CCCGGG 1 cut(s) 1824
SmiMI CAYNNNNRTG 3 cut(s) 968, 2573, 3180
SmlI CTYRAG 4 cut(s) 847, 1982, 1991, 2528
SmoI CTYRAG 4 cut(s) 847, 1982, 1991, 2528
SphI GCATGC 1 cut(s) 376
SsiI CCGC 5 cut(s) 18, 275, 1530, 2082, 2649
SspI AATATT 1 cut(s) 2356
StyD4I CCNGG 6 cut(s) 476, 1617, 1753, 1821, 1822, 2674
StyI CCWWGG 5 cut(s) 678, 762, 2476, 2509, 2585
TaaI ACNGT 7 cut(s) 721, 1442, 1461, 1761, 2142, 2575, 3137
TaiI ACGT 6 cut(s) 132, 981, 1080, 2436, 2716, 3113
TaqII GACCGA 1 cut(s) 204
TatI WGTACW 4 cut(s) 616, 2142, 2734, 3132
TauI GCSGC 1 cut(s) 278
TfiI GAWTC 5 cut(s) 68, 902, 1136, 1996, 2590
Tru1I TTAA 9 cut(s) 213, 459, 807, 1370, 1853, 1913, 1937, 2240, 2940
Tru9I TTAA 9 cut(s) 213, 459, 807, 1370, 1853, 1913, 1937, 2240, 2940
TscAI CASTG 5 cut(s) 512, 724, 1123, 2272, 2580
TseFI GTSAC 3 cut(s) 829, 2575, 2861
TseI GCWGC 9 cut(s) 62, 80, 326, 1041, 1298, 1422, 2334, 2885, 2996
Tsp45I GTSAC 3 cut(s) 829, 2575, 2861
TspGWI ACGGA 3 cut(s) 1087, 2460, 3215
TspMI CCCGGG 1 cut(s) 1822
TspRI CASTG 5 cut(s) 512, 724, 1123, 2272, 2580
VpaK11BI GGWCC 2 cut(s) 149, 2909
XagI CCTNNNNNAGG 1 cut(s) 2416
XapI RAATTY 8 cut(s) 387, 599, 855, 1534, 1558, 1881, 2236, 3201
XbaI TCTAGA 1 cut(s) 923
XceI RCATGY 3 cut(s) 376, 776, 1030
XcmI CCANNNNNNNNNTGG 1 cut(s) 340
XmaI CCCGGG 1 cut(s) 1822
XmiI GTMKAC 3 cut(s) 585, 1231, 2323
XmnI GAANNNNTTC 2 cut(s) 919, 3239
ZrmI AGTACT 1 cut(s) 618
Zsp2I ATGCAT 2 cut(s) 374, 1608
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.