MD11G1019800.v1.1

disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000085
Physical Location & Seq
Reverse (-)
1687459 .. 1691455
3997 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1019800.v1.1.491

Sequence Viewer

Length: 3159 bp
ATGGAAATTTGTAGTTTTGTACTACGAGTTGGTGAGTTGTTATGGACTCCAGCGAAGCGTAATGTGGGTTACTTGGTGCATTACAAGAGACACATACAGTCTCTGGAAAAACTGATAGAAAAGCTTGAGACCACGCAGAATGATTACCAGCAAAGTGTGGATGCGGCGTTAATGAATGGAGACAAAGTTAAGTCCGAGGTTCAAAAATGGCTCAAGGATGTTGACAAGGCCATAAACGATGCCAAAAGACTGAATAATGAAGCCGGAGAAGACAAAACATGCCTCGGAGGCTGCTGTCCGAATTTGAAATGGCGTTACGGTTTAAGCAAGAGAGCCGTTAAGGAGGCAGAAGAAATGAACAAGCTTAATGAAGAGAAAAGGTTTGAAACAGTTTCGCTTCAAGTTCACCTACCCGTTGAGTTTGAGACCATGTCAACTGGAGATTTCGAAGCATTTGAAGCAACAAGGCAAGCCATGGATCAGGTCATGAAGGCACTGAAAGACGATAACGTCACTGTCATTGCGGTCCATGGAATGGGAGGCATAGGCAAGACGACCATGGTGAAACATGTCGGTGTGCAAGTGGGTAAAGGCAAGCTCTTTGATCATGTGATTATGGCTGTCGTTTCCCAAAACCCAAACCTGGTAAAGATTCAACAACAGCTTGCAGAAATGCTGGACTTGAATTTGAATCAGCAGACAGAAATAGCCAGAGCAGCTAGATTGAAGGAGAGAATAATGAGAGGAAAAAAGATCCTTATAATTTTGGACGACATTTGGAAGACAATAGATTTGTCTCTCATAGGGATCCCCGATCACTACGAGCTCCAAAACTGCAAGGCCAAAGTTCTACTCACCACAAGGATAAGGAATGTATGTCATGCCATGAAGAGCCAAGAAAAGATTCATCTCAATATCCTATCAGAAGAAGATTCTTGGGCCTTGTTTGTGAAAAAGGCAAACAGGTCTTTTGAATCAACCAATTTCTATGACATAGCGAGGAAGGTGGCTGGAGAATGTGGTGGTCTCCCGATTGCTTTGATTGCAGTTGCAAGAGCACTCGGAGACAAGGACTTGGAAGAATGGAGAGAAGCAGCTCGACGACTAGAGATGTCCCAAACTACCAACCTTGATGACGAGGGGGATGTGTTCAGATGTATAAAGCTGAGCTACGATTACTTGAATGGCAATGACGCCAAGTCATTTTTCTTATTTTGCTGCTTATTCCCGGAGGACTCTGATATCCAAATTGAAGACTTGCTGAAGTATGGTATTGGGAAAGGATTGTTTCGAGATGCCAACTCAATGCAAGAAGCCAGAAGCACAGCACATTCGGTGGCCAAGTACCTCAAAGCTTGTAGCTTGGTTTTGGATGGTGAAGAAGACGGATGTGTAAGAATGCATGATGTGATTCGGGACATGGCAATACTAATTGCATCATCTGGGGATGGCCATGGATTCTTCGTAAAAGCTGGCTGCGACTTAAAGGATTGGCCAAAGAATTCACTCGAAGACTACTCTGCAATCTCCCTAATGGGGAATGAAATCTGCAATCTTCCTGACGAGTTGGTATGTCCAAAACTCCAGATTTTATTACTACAGTCAAATTTTGAAGTAGAGGAGATCCCAGAAGATTTTTTCCAAAGCCCAAATGCATTAAGGGTATTCGACATAAGTAGTACCCAAATTTCTTCATTACCCTCATCATTTAATCTCTTAACCAAGCTCCAAGCGCTGCATCTAGATAATTGTCAGACAATAACGGATGTATCAATTCTCGGAGAACTGAAAAAACTGGAGGTTCTTAGTATGAGAGAATCTGTTATTGAGGAATTGCCAGAAGAATTTGGACATTTGACCAATTTAAGGATGTTGGATCTCACTTTGAGTGCCTACATTGCAACAATTCCATCCAATGTGATATCGCGGCTGTCCAAATTAGAAGAACTATACATGCAACAGAGTTTTGCTGACTGGGGCAAGAAAGTTGAAGGAGAAGATGACAAAACTAATGCTAGTTTTGATGAGCTAATTAGCTTGTGTTACTTAAACATTTTGAAGGTTGACATTTCTGATGCAGAATGCCTTCCGAAAAATGTAGGGTTCCTTCGAAACTGGGCAAATTTTGATATATGTATCAGCAGAAACCAATTTCATCGGTCCATAAATGTGCAATTATCAAAAAATACTATTACTCCACATTCAACTTCAAGAGCCTTGCTTCTGGACACAACAATCAATACGCTGCCAGATTGGTTTAACATCACAGTGACGGAGAGAGCGGAGAAAATAATCTACATGGAGCCTAGAAGTTTAACTAACATTCTTGTGGAGTACGATCATTGGAAGTCACATGGACTAAAGTTTTTGCATATCCAAGCGTGCAGCGAGATGGTAACTTTGATGAACACAGTAACTAGGGTTCCAAATAAGCCTGTGCTCGAGAGCTTGGAAGAGTTGCGTCTCTTCTTATGGGACTGCTTGCAGCAGTTATGTGTTGGTGAATTACCGCAGGGGTCGCTTGGAAATCTCAAGATCTTGGGGGTGGAGCAGTGTGATGGTGTGACGGATGCACTTGTTCCATCTAATCTGTTGCGGAGACTACAAAATCTTGAAGTACTTATTGTAGGTGGAGCCGACATGGTTTATACATTCAGATCTCAAGGGCTTGAACAAGGAGAAACGGTCTTGACAAAATTGAGAGAGATGAAACTGGAGAATCTACCAGAACTGACAAACATATGGAACGGTCCTGCTCAGCCTGCAATGTTTCATAATCTTAAAACTTTGATAATTTCCAAGTGCAAGAAACTGAAAACTCTCTTCACATTCGACATAGCTCAATGTCTGCTGCAATTGGAAGAGCTTTGGGTGGAGGAATGCCATAGCTTGGACAAACTTTTCGGCGTAAGTGAGGGATTAATAGTGCAGGATGACGAGATCATTTTTCCACAACTGAAGAACTTAGCATTGCAAAGTCTTTCAAAGCTGACAAGCGTACTCGCTGGAAGTGCTACACTTTTGTGCCCTTCGTTGGAATACTTGCATGTGCTGGAGTGCCCTGAGTTTTTAACCTCTACTTCTGACTTCCACAGCAATATGCAAGTCCAAGTAAACAATGAGCAACATTTCCTCTTCCTAAGGAAAAGAGGTAATTAA

Protein Analysis

1053

Amino Acids

119.0

Weight (kDa)

5.34

Isoelectric Point (pI)

45.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 157 - 322 5.4e-34 NB-ARC domain
LRR_8 PF13855 526 - 585 2.1e-08 Leucine rich repeat
LRR_14 PF23598 532 - 650 6.5e-13 Leucine-rich repeat region
LRR_14 PF23598 593 - 755 4.9e-09 Leucine-rich repeat region
LRR_RPS2 PF23247 817 - 961 1.8e-30 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 761
AasI GACNNNNNNGTC 1 cut(s) 509
AccB7I CCANNNNNTGG 2 cut(s) 535, 2890
AccBSI CCGCTC 1 cut(s) 2282
AccII CGCG 1 cut(s) 1927
AciI CCGC 6 cut(s) 164, 524, 1927, 2282, 2510, 2596
AclWI GGATC 6 cut(s) 486, 748, 802, 815, 1618, 1884
AcoI YGGCCR 3 cut(s) 1338, 1450, 1493
AcsI RAATTY 8 cut(s) 6, 301, 685, 1501, 1606, 1686, 1844, 2122
AcuI CTGAAG 2 cut(s) 1283, 2978
AcyI GRCGYC 1 cut(s) 1194
AfaI GTAC 6 cut(s) 21, 1346, 1681, 2336, 2619, 3000
AfeI AGCGCT 1 cut(s) 1734
AfiI CCNNNNNNNGG 6 cut(s) 535, 643, 1536, 1866, 2890, 3034
AflIII ACRYGT 1 cut(s) 568
AhdI GACNNNNNGTC 1 cut(s) 1198
AjnI CCWGG 1 cut(s) 642
AjuI GAANNNNNNNTTGG 4 cut(s) 888, 920, 1190, 1222
AleI CACNNNNGTG 1 cut(s) 3022
Alw21I GWGCWC 3 cut(s) 828, 1060, 2442
AlwI GGATC 6 cut(s) 486, 748, 802, 815, 1618, 1884
AlwNI CAGNNNCTG 1 cut(s) 103
Ama87I CYCGRG 1 cut(s) 2441
Aor51HI AGCGCT 1 cut(s) 1734
AoxI GGCC 6 cut(s) 228, 840, 939, 1338, 1450, 1493
ApoI RAATTY 8 cut(s) 6, 301, 685, 1501, 1606, 1686, 1844, 2122
ArsI GACNNNNNNTTYG 4 cut(s) 775, 807, 2885, 2917
AseI ATTAAT 1 cut(s) 2921
Asp700I GAANNNNTTC 2 cut(s) 903, 2085
AspLEI GCGC 1 cut(s) 1735
AspS9I GGNCC 4 cut(s) 526, 939, 2160, 2750
AsuC2I CCSGG 1 cut(s) 1229
AsuHPI GGTGA 6 cut(s) 44, 398, 574, 847, 1388, 2513
AsuII TTCGAA 2 cut(s) 447, 2110
AvaI CYCGRG 1 cut(s) 2441
AvaII GGWCC 3 cut(s) 526, 2160, 2750
AxyI CCTNAGG 1 cut(s) 3140
BaeGI GKGCMC 2 cut(s) 3029, 3062
BaeI ACNNNNGTAYC 2 cut(s) 1752, 1785
BalI TGGCCA 3 cut(s) 1340, 1452, 1495
BamHI GGATCC 1 cut(s) 807
BanII GRGCYC 1 cut(s) 828
BarI GAAGNNNNNNTAC 2 cut(s) 2091, 2123
BbsI GAAGAC 5 cut(s) 276, 788, 1260, 1389, 1518
Bbv12I GWGCWC 3 cut(s) 828, 1060, 2442
BccI CCATC 6 cut(s) 1367, 1442, 1918, 2386, 2552, 2590
BceAI ACGGC 1 cut(s) 320
BciT130I CCWGG 1 cut(s) 644
BclI TGATCA 1 cut(s) 604
BcnI CCSGG 1 cut(s) 1229
BfaI CTAG 6 cut(s) 720, 1106, 1742, 2016, 2307, 2418
BfmI CTRYAG 1 cut(s) 1598
BfoI RGCGCY 1 cut(s) 1736
BglI GCCNNNNNGGC 1 cut(s) 288
BglII AGATCT 2 cut(s) 2535, 2657
BlpI GCTNAGC 2 cut(s) 1166, 2757
BmcAI AGTACT 1 cut(s) 2619
Bme1390I CCNGG 2 cut(s) 644, 1229
Bme18I GGWCC 3 cut(s) 526, 2160, 2750
BmeRI GACNNNNNGTC 1 cut(s) 1198
BmeT110I CYCGRG 1 cut(s) 2441
BmgT120I GGNCC 4 cut(s) 526, 939, 2160, 2750
BmiI GGNNCC 5 cut(s) 809, 2105, 2304, 2424, 2635
BmrFI CCNGG 2 cut(s) 644, 1229
BmrI ACTGGG 2 cut(s) 1984, 2125
BmsI GCATC 7 cut(s) 151, 229, 1285, 1445, 1747, 2065, 2560
BmuI ACTGGG 2 cut(s) 1984, 2125
BpiI GAAGAC 5 cut(s) 276, 788, 1260, 1389, 1518
BpmI CTGGAG 7 cut(s) 33, 459, 1032, 1568, 1817, 2735, 3074
Bpu1102I GCTNAGC 2 cut(s) 1166, 2757
Bpu14I TTCGAA 2 cut(s) 447, 2110
BpuEI CTTGAG 4 cut(s) 146, 197, 2516, 2646
BpuMI CCSGG 1 cut(s) 1229
BsaHI GRCGYC 1 cut(s) 1194
BsaI GGTCTC 3 cut(s) 122, 419, 1031
BsaJI CCNNGG 6 cut(s) 195, 283, 474, 529, 558, 1453
BsaXI ACNNNNNCTCC 6 cut(s) 171, 201, 2179, 2209, 2670, 2700
Bsc4I CCNNNNNNNGG 6 cut(s) 535, 643, 1536, 1866, 2890, 3034
Bse1I ACTGG 5 cut(s) 442, 1800, 1979, 2120, 2718
Bse21I CCTNAGG 1 cut(s) 3140
Bse3DI GCAATG 5 cut(s) 519, 1195, 1896, 2772, 2969
BseBI CCWGG 1 cut(s) 644
BseDI CCNNGG 6 cut(s) 195, 283, 474, 529, 558, 1453
BseLI CCNNNNNNNGG 6 cut(s) 535, 643, 1536, 1866, 2890, 3034
BseMI GCAATG 5 cut(s) 519, 1195, 1896, 2772, 2969
BseMII CTCAG 3 cut(s) 1157, 2771, 3054
BseNI ACTGG 5 cut(s) 442, 1800, 1979, 2120, 2718
BseRI GAGGAG 1 cut(s) 1634
BseSI GKGCMC 2 cut(s) 3029, 3062
BsgI GTGCAG 2 cut(s) 2404, 2948
Bsh1236I CGCG 1 cut(s) 1927
BshFI GGCC 6 cut(s) 230, 842, 941, 1340, 1452, 1495
BsiHKAI GWGCWC 3 cut(s) 828, 1060, 2442
BsiHKCI CYCGRG 1 cut(s) 2441
BsiSI CCGG 2 cut(s) 264, 1229
BslFI GGGAC 3 cut(s) 1099, 1430, 2489
BslI CCNNNNNNNGG 6 cut(s) 535, 643, 1536, 1866, 2890, 3034
BsmBI CGTCTC 1 cut(s) 2468
BsmFI GGGAC 3 cut(s) 1099, 1430, 2489
BsmI GAATGC 3 cut(s) 1404, 2087, 2885
BsnI GGCC 6 cut(s) 230, 842, 941, 1340, 1452, 1495
Bso31I GGTCTC 3 cut(s) 122, 419, 1031
BsoBI CYCGRG 1 cut(s) 2441
Bsp119I TTCGAA 2 cut(s) 447, 2110
Bsp1286I GDGCHC 5 cut(s) 828, 1060, 2442, 3029, 3062
Bsp1720I GCTNAGC 2 cut(s) 1166, 2757
Bsp19I CCATGG 4 cut(s) 474, 529, 558, 1453
BspACI CCGC 6 cut(s) 164, 524, 1927, 2282, 2510, 2596
BspANI GGCC 6 cut(s) 230, 842, 941, 1340, 1452, 1495
BspCNI CTCAG 3 cut(s) 1158, 2770, 3055
BspFNI CGCG 1 cut(s) 1927
BspHI TCATGA 1 cut(s) 486
BspLI GGNNCC 5 cut(s) 809, 2105, 2304, 2424, 2635
BspPI GGATC 6 cut(s) 486, 748, 802, 815, 1618, 1884
BspQI GCTCTTC 2 cut(s) 884, 2856
BspT104I TTCGAA 2 cut(s) 447, 2110
BspTNI GGTCTC 3 cut(s) 122, 419, 1031
BsrBI CCGCTC 1 cut(s) 2282
BsrDI GCAATG 5 cut(s) 519, 1195, 1896, 2772, 2969
BsrI ACTGG 5 cut(s) 442, 1800, 1979, 2120, 2718
BssECI CCNNGG 6 cut(s) 195, 283, 474, 529, 558, 1453
BssNI GRCGYC 1 cut(s) 1194
BssT1I CCWWGG 4 cut(s) 474, 529, 558, 1453
Bst2UI CCWGG 1 cut(s) 644
Bst4CI ACNGT 9 cut(s) 99, 320, 391, 517, 1602, 2269, 2413, 2686, 2750
Bst6I CTCTTC 7 cut(s) 366, 884, 2448, 2471, 2828, 2856, 3140
BstACI GRCGYC 1 cut(s) 1194
BstBI TTCGAA 2 cut(s) 447, 2110
BstC8I GCNNGC 7 cut(s) 471, 596, 666, 1474, 2383, 2483, 2763
BstDEI CTNAG 6 cut(s) 1166, 1805, 2757, 2965, 3063, 3140
BstDSI CCRYGG 4 cut(s) 474, 529, 558, 1453
BstFNI CGCG 1 cut(s) 1927
BstH2I RGCGCY 1 cut(s) 1736
BstHHI GCGC 1 cut(s) 1735
BstMWI GCNNNNNNNGC 9 cut(s) 288, 458, 716, 1043, 1732, 1898, 2518, 2762, 3011
BstNI CCWGG 1 cut(s) 644
BstNSI RCATGY 4 cut(s) 282, 572, 1957, 3050
BstSCI CCNGG 2 cut(s) 642, 1227
BstSFI CTRYAG 1 cut(s) 1598
BstSLI GKGCMC 2 cut(s) 3029, 3062
BstUI CGCG 1 cut(s) 1927
BstV2I GAAGAC 5 cut(s) 276, 788, 1260, 1389, 1518
BstX2I RGATCY 6 cut(s) 753, 807, 1623, 1876, 2535, 2657
BstYI RGATCY 6 cut(s) 753, 807, 1623, 1876, 2535, 2657
Bsu36I CCTNAGG 1 cut(s) 3140
BsuRI GGCC 6 cut(s) 230, 842, 941, 1340, 1452, 1495
BtgI CCRYGG 4 cut(s) 474, 529, 558, 1453
BtsI GCAGTG 1 cut(s) 2558
BtsIMutI CAGTG 4 cut(s) 494, 513, 2274, 2558
Cac8I GCNNGC 7 cut(s) 471, 596, 666, 1474, 2383, 2483, 2763
CaiI CAGNNNCTG 1 cut(s) 103
CciI TCATGA 1 cut(s) 486
CfoI GCGC 1 cut(s) 1735
Cfr13I GGNCC 4 cut(s) 526, 939, 2160, 2750
CseI GACGC 2 cut(s) 1202, 2450
CsiI ACCWGGT 1 cut(s) 642
Csp6I GTAC 6 cut(s) 20, 1345, 1680, 2335, 2618, 2999
CviQI GTAC 6 cut(s) 20, 1345, 1680, 2335, 2618, 2999
DdeI CTNAG 6 cut(s) 1166, 1805, 2757, 2965, 3063, 3140
DrdI GACNNNNNNGTC 1 cut(s) 509
DriI GACNNNNNGTC 1 cut(s) 1198
DseDI GACNNNNNNGTC 1 cut(s) 509
EaeI YGGCCR 3 cut(s) 1338, 1450, 1493
Eam1104I CTCTTC 7 cut(s) 366, 884, 2448, 2471, 2828, 2856, 3140
Eam1105I GACNNNNNGTC 1 cut(s) 1198
EarI CTCTTC 7 cut(s) 366, 884, 2448, 2471, 2828, 2856, 3140
Ecl136II GAGCTC 1 cut(s) 826
Eco130I CCWWGG 4 cut(s) 474, 529, 558, 1453
Eco24I GRGCYC 1 cut(s) 828
Eco31I GGTCTC 3 cut(s) 122, 419, 1031
Eco32I GATATC 2 cut(s) 1243, 1923
Eco47I GGWCC 3 cut(s) 526, 2160, 2750
Eco47III AGCGCT 1 cut(s) 1734
Eco53kI GAGCTC 1 cut(s) 826
Eco57I CTGAAG 2 cut(s) 1283, 2978
Eco81I CCTNAGG 1 cut(s) 3140
Eco88I CYCGRG 1 cut(s) 2441
EcoICRI GAGCTC 1 cut(s) 826
EcoRI GAATTC 1 cut(s) 1501
EcoRII CCWGG 1 cut(s) 642
EcoRV GATATC 2 cut(s) 1243, 1923
EcoT14I CCWWGG 4 cut(s) 474, 529, 558, 1453
EcoT22I ATGCAT 2 cut(s) 1404, 1657
EcoT38I GRGCYC 1 cut(s) 828
ErhI CCWWGG 4 cut(s) 474, 529, 558, 1453
Esp3I CGTCTC 1 cut(s) 2468
FaqI GGGAC 3 cut(s) 1099, 1430, 2489
FauNDI CATATG 1 cut(s) 2741
FbaI TGATCA 1 cut(s) 604
FriOI GRGCYC 1 cut(s) 828
FspBI CTAG 6 cut(s) 720, 1106, 1742, 2016, 2307, 2418
GlaI GCGC 1 cut(s) 1734
GsuI CTGGAG 7 cut(s) 33, 459, 1032, 1568, 1817, 2735, 3074
HaeII RGCGCY 1 cut(s) 1736
HaeIII GGCC 6 cut(s) 230, 842, 941, 1340, 1452, 1495
HapII CCGG 2 cut(s) 264, 1229
HgaI GACGC 2 cut(s) 1202, 2450
HhaI GCGC 1 cut(s) 1735
Hin1I GRCGYC 1 cut(s) 1194
Hin6I GCGC 1 cut(s) 1733
HinP1I GCGC 1 cut(s) 1733
HincII GTYRAC 3 cut(s) 223, 435, 2065
HindII GTYRAC 3 cut(s) 223, 435, 2065
HindIII AAGCTT 3 cut(s) 122, 362, 1353
HpaII CCGG 2 cut(s) 264, 1229
HphI GGTGA 6 cut(s) 44, 398, 574, 847, 1388, 2513
Hpy166II GTNNAC 5 cut(s) 223, 406, 435, 2065, 3115
Hpy8I GTNNAC 5 cut(s) 223, 406, 435, 2065, 3115
Hpy99I CGWCG 1 cut(s) 1104
HpyAV CCTTC 8 cut(s) 484, 721, 997, 1985, 2053, 2096, 2117, 3039
HpyCH4III ACNGT 9 cut(s) 99, 320, 391, 517, 1602, 2269, 2413, 2686, 2750
HpyCH4IV ACGT 1 cut(s) 510
HpyF10VI GCNNNNNNNGC 9 cut(s) 288, 458, 716, 1043, 1732, 1898, 2518, 2762, 3011
HpyF3I CTNAG 6 cut(s) 1166, 1805, 2757, 2965, 3063, 3140
HpySE526I ACGT 1 cut(s) 510
Hsp92I GRCGYC 1 cut(s) 1194
HspAI GCGC 1 cut(s) 1733
Ksp22I TGATCA 1 cut(s) 604
LguI GCTCTTC 2 cut(s) 884, 2856
LmnI GCTCC 5 cut(s) 831, 1731, 2302, 2548, 2633
LweI GCATC 7 cut(s) 151, 229, 1285, 1445, 1747, 2065, 2560
MabI ACCWGGT 1 cut(s) 642
MaeI CTAG 6 cut(s) 720, 1106, 1742, 2016, 2307, 2418
MaeII ACGT 1 cut(s) 510
MaeIII GTNAC 9 cut(s) 68, 314, 511, 2042, 2269, 2349, 2395, 2413, 2563
MbiI CCGCTC 1 cut(s) 2282
MfeI CAATTG 1 cut(s) 2855
MflI RGATCY 6 cut(s) 753, 807, 1623, 1876, 2535, 2657
MhlI GDGCHC 5 cut(s) 828, 1060, 2442, 3029, 3062
MlsI TGGCCA 3 cut(s) 1340, 1452, 1495
MluNI TGGCCA 3 cut(s) 1340, 1452, 1495
MlyI GAGTC 2 cut(s) 40, 1229
MmeI TCCRAC 2 cut(s) 1854, 3015
Mox20I TGGCCA 3 cut(s) 1340, 1452, 1495
Mph1103I ATGCAT 2 cut(s) 1404, 1657
MroXI GAANNNNTTC 2 cut(s) 903, 2085
MscI TGGCCA 3 cut(s) 1340, 1452, 1495
MslI CAYNNNNRTG 5 cut(s) 573, 2168, 2267, 2327, 3022
Msp20I TGGCCA 3 cut(s) 1340, 1452, 1495
MspI CCGG 2 cut(s) 264, 1229
MspR9I CCNGG 2 cut(s) 644, 1229
MunI CAATTG 1 cut(s) 2855
Mva1269I GAATGC 3 cut(s) 1404, 2087, 2885
MvaI CCWGG 1 cut(s) 644
MvnI CGCG 1 cut(s) 1927
MwoI GCNNNNNNNGC 9 cut(s) 288, 458, 716, 1043, 1732, 1898, 2518, 2762, 3011
NciI CCSGG 1 cut(s) 1229
NcoI CCATGG 4 cut(s) 474, 529, 558, 1453
NdeI CATATG 1 cut(s) 2741
NlaIV GGNNCC 5 cut(s) 809, 2105, 2304, 2424, 2635
NmuCI GTSAC 4 cut(s) 511, 2269, 2349, 2563
NsiI ATGCAT 2 cut(s) 1404, 1657
NspI RCATGY 4 cut(s) 282, 572, 1957, 3050
NspV TTCGAA 2 cut(s) 447, 2110
OliI CACNNNNGTG 1 cut(s) 3022
PaeR7I CTCGAG 1 cut(s) 2441
PagI TCATGA 1 cut(s) 486
PciI ACATGT 1 cut(s) 568
PciSI GCTCTTC 2 cut(s) 884, 2856
PctI GAATGC 3 cut(s) 1404, 2087, 2885
PdmI GAANNNNTTC 2 cut(s) 903, 2085
PfeI GAWTC 9 cut(s) 652, 691, 904, 932, 974, 1411, 1458, 1817, 2719
PflFI GACNNNGTC 1 cut(s) 430
PflMI CCANNNNNTGG 2 cut(s) 535, 2890
PfoI TCCNGGA 1 cut(s) 1227
PleI GAGTC 2 cut(s) 40, 1229
PpsI GAGTC 2 cut(s) 40, 1229
PscI ACATGT 1 cut(s) 568
PshBI ATTAAT 1 cut(s) 2921
PsiI TTATAA 1 cut(s) 761
Psp124BI GAGCTC 1 cut(s) 828
Psp6I CCWGG 1 cut(s) 642
PspGI CCWGG 1 cut(s) 642
PspN4I GGNNCC 5 cut(s) 809, 2105, 2304, 2424, 2635
PspPI GGNCC 4 cut(s) 526, 939, 2160, 2750
PsrI GAACNNNNNNTAC 2 cut(s) 2406, 2438
PstNI CAGNNNCTG 1 cut(s) 103
PsuI RGATCY 6 cut(s) 753, 807, 1623, 1876, 2535, 2657
PsyI GACNNNGTC 1 cut(s) 430
RsaI GTAC 6 cut(s) 21, 1346, 1681, 2336, 2619, 3000
RsaNI GTAC 6 cut(s) 20, 1345, 1680, 2335, 2618, 2999
RseI CAYNNNNRTG 5 cut(s) 573, 2168, 2267, 2327, 3022
SacI GAGCTC 1 cut(s) 828
SapI GCTCTTC 2 cut(s) 884, 2856
Sau96I GGNCC 4 cut(s) 526, 939, 2160, 2750
ScaI AGTACT 1 cut(s) 2619
SchI GAGTC 2 cut(s) 40, 1229
ScrFI CCNGG 2 cut(s) 644, 1229
SduI GDGCHC 5 cut(s) 828, 1060, 2442, 3029, 3062
SexAI ACCWGGT 1 cut(s) 642
SfaNI GCATC 7 cut(s) 151, 229, 1285, 1445, 1747, 2065, 2560
SfcI CTRYAG 1 cut(s) 1598
Sfr274I CTCGAG 1 cut(s) 2441
SfuI TTCGAA 2 cut(s) 447, 2110
SinI GGWCC 3 cut(s) 526, 2160, 2750
SlaI CTCGAG 1 cut(s) 2441
SmiMI CAYNNNNRTG 5 cut(s) 573, 2168, 2267, 2327, 3022
SmlI CTYRAG 5 cut(s) 125, 212, 2441, 2531, 2661
SmoI CTYRAG 5 cut(s) 125, 212, 2441, 2531, 2661
SsiI CCGC 6 cut(s) 164, 524, 1927, 2282, 2510, 2596
SspMI CTAG 6 cut(s) 720, 1106, 1742, 2016, 2307, 2418
SstI GAGCTC 1 cut(s) 828
StyD4I CCNGG 2 cut(s) 642, 1227
StyI CCWWGG 4 cut(s) 474, 529, 558, 1453
TaaI ACNGT 9 cut(s) 99, 320, 391, 517, 1602, 2269, 2413, 2686, 2750
TaiI ACGT 1 cut(s) 513
TaqI TCGA 8 cut(s) 447, 1099, 1291, 1509, 1668, 2110, 2442, 2832
TaqII GACCGA 1 cut(s) 2148
TatI WGTACW 2 cut(s) 19, 2617
TauI GCSGC 2 cut(s) 167, 1930
TfiI GAWTC 9 cut(s) 652, 691, 904, 932, 974, 1411, 1458, 1817, 2719
TscAI CASTG 4 cut(s) 501, 520, 2274, 2558
TseFI GTSAC 4 cut(s) 511, 2269, 2349, 2563
Tsp45I GTSAC 4 cut(s) 511, 2269, 2349, 2563
TspGWI ACGGA 4 cut(s) 1401, 1778, 2288, 2582
TspRI CASTG 4 cut(s) 501, 520, 2274, 2558
Tth111I GACNNNGTC 1 cut(s) 430
Van91I CCANNNNNTGG 2 cut(s) 535, 2890
VpaK11BI GGWCC 3 cut(s) 526, 2160, 2750
VspI ATTAAT 1 cut(s) 2921
XapI RAATTY 8 cut(s) 6, 301, 685, 1501, 1606, 1686, 1844, 2122
XbaI TCTAGA 1 cut(s) 1741
XceI RCATGY 4 cut(s) 282, 572, 1957, 3050
XhoI CTCGAG 1 cut(s) 2441
XmnI GAANNNNTTC 2 cut(s) 903, 2085
XspI CTAG 6 cut(s) 720, 1106, 1742, 2016, 2307, 2418
ZrmI AGTACT 1 cut(s) 2619
Zsp2I ATGCAT 2 cut(s) 1404, 1657
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.