RchiOBHm_Chr3g0473291

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000085
Physical Location & Seq
Forward (+)
19180122 .. 19185269
5148 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43897

Sequence Viewer

Length: 3696 bp
ATGGAGTTCTACCAGTATGTCTTTGAGGCTATGAATATGAAGATGATTATTGTGATTAGTGCGGTAATGGTGCAATGGTTCGGTGTCATAGCCTGTCTTAAGTGGATATGGAAGAATTGGATCCTTATTGTGGTGATTGTGACATGGAACGTAATAGCCAGTCTTGGGTGGATATGGAAGAGTAGGTTCAGTTACATGGTTGAAACCCCAGAGCCAATATCAGAGGATAATCAAGAACCAGTTGACGTCGATGTCTCTGCATCTCTTCCTTCATCATCAGCTGAATTAACAGATCCTACGTGGAAGCATGATGTGTTTTTGAGCTTCAGGGGTGTAGACACTCGTAAGAATATTACCTTCTTTTTAAACAAACGGCTGCTAGAAAGGGGAATCAAAACATTCAAGGATGAACAAAACATTGAAGTAGGGGATGTTATTTCTCCCACTCTCCTAACGGCAATTGAAGAATCAAGGTTGGCAATTATTGTTCTCTCTCAAAACTATGCCTCTTCCTCTTGGTGTTTGGAGGAACTAGGACACATTTGTAAAAGCATGAAAGGAGACGAGTCAGACGACAATAGAATTCTGCCACTTTTTTATAATGTGCATCCTAGTGATGTACGACATCAGACGAGCAGGTTCGGAGATGCTTTCACTAAGCTTAAAAAATCTGGGCGACACGAATCAGCGAAGGTGAAGCAGTGGATAGATACTTTAACAAAAGTTGCACAAATCTCCGGGTGGCATTCACTGGAATTTAAAAATGACGGAGAACTTTTGAATAACATTGTGGAAGCTGTGTGCAAGAAATTACCACCGACTGAATCTGTGTTAAAAATGTCCCTCGGAGATTTTGAAGAATTTGCAGCAACAAAAGAAGCCATTCATGAGGTCATGAAGGCGCTAAAAGATGGTGAGGCCACTACCATTGGAGTCTACGGCATGGGGGGCGTTGGAAAGACAACAATGGTGAAACATGTCGGTGTACAAGCCCAAGAAGAAAAGCTTTTTAATAAAGTGATTATGGCTGTCGTATCCCAAAAACCCGACTTGAAGGAAATTCAACAGGAATTTGCAGAAACGCTGGGCTTTGAACTCAAGAAGAAGACGGAAAGTGTAAGAGCCCGTGAATTGAAGAAGAAAATAATGGAAGAAACCAGGATCCTCATAATCTTGGATGACATTTGGAAGGGAATAGACTTTTCAAGAATTGGAATTCCGAGCCACAACGAACTTCAAAAATGCAATTCCAAAGTCTTATTGACCACCAGAAGATTGAAAGTTTGTAGTATCATGGGGAGCCATGCAAAAATTCCTCTCAAAATCTTATCAGAAGCAGATTCTTGGCGCTTATTTGTGAGGGAAGCGGGGATGTCTTCTGACAAATCTCCCAATTTCTATGATGTAGCGAGTGAGGTGGCAAGAGAATGCGCCGGTCTACCTGTTGCTTTGATAGCAGTTGCGAGGGCCCTTCGAGATGAAGGTTTGGACAGATGGAAAGAAGCTGCTCGACGACTAAAATCTTCTCAACCTCCCATCCGTGAAGATGAGAAAGCTGTGTTCAAATGCATAAAGTTAAGCTATGATTTCTTGAAGGATGATGAATTGAAATCATGCTTCTTGCTTTGCTGCTTGTTCCCAGAAGATTATGATATCCCAATTGAATACTTGGTCATGTATGGAATTGGGAAAGGAATGTTTCGAGATTTAACCATGCTACAAGCCCGAGAGTCAACATATTCAGTGGTGCAGGACCTTGAAGATTCTAGCTTGCTTTTAGGCGGTAGAGATGACGGATGTGTAAGGATGCATGATGTCATTCGGGATATGGCAATATTAATGACATCATTATCTGAAGACGGCCAGCGGTTCTTGGTGAAAGTTGGCTGTGAATTGACGAATTGGCCAGAGATTGATGCACATAAAGGCTACTCTGCAATCTCACTAATGAAGAACAAAATTTGCGAGCTACCAGAAGAGTTGGTATGTCCAAATCTCCAGATTTTATCACTACGACAGAATGCTAATTTAAATGATATCCCAAAATCTTTTTTCCAAAGTAAGAATGAATTAAGAGTCTTGGATCTTAGCAACACTCGTATTTCATCACTACCCCAATCAATCGTTTTCCTAACCAACCTTCAAGCTTTGTATTTAGATTATTGCAGAAACATAACGGACACTTCTGTAATCGGAAAACTTAACAAGCTTGAGATTCTTAGTATGAGAGGAACTGCTCTGAAAAAATTGTCAAGGGAAATAGAACAACTGACCAATCTAAGGATGCTCGATATAAGTGCTGCAGCTTTATCCTTGGGAGGAAGTATTTGCACAATTCCATCTAAAGTGATATCAAAGTTGCATAAATTAGAAGAGCTGTACATGCAATATGGATTTTGGGACTGGGGGAGTAAAATTGATGGAAAAGGAGAAGAAACTAATATTGGCTTTGATGAATTAGCTAGTTTATCATATTTAAGAATTTTGAAAGTTTGCATATTAGATGCAAATTGCATCCCTAAAAGTGTTAAGGTCGAACCGAAGTGGGTTTACTTTGATATTAGTATTTGCAGCGGCAAGAGTGGACAGGGAATCTATAACATCCTTCGATCTGGTAATAATTATAGATCCTTGAGTCTTGATGGTACTGACACAACCATGGGAACCCTTCCGGACTGGTTTGTCAACGCTGTGGTAAAGAAAACTGAGAGGCTGAGTTATGAAGAATGCACAGGGTTGAGTAACATTCTTGTGGAATATGACCGTGGGAGGTTGCATGGACTGAAAGTTCTCTCTGTAATTAACTGTGAGAACTTGAAAGAATTGATGAATGCAATAACATGTGTTCCAGATATGCGTATGCCTGTGTTTGAGAACTTGGAAGAGTTGTATCTGGAAGAACTGGATGACCTGAAGCAGTTATGTGTTGGTGAGTTACCACCTGGATCTCTCTGCAGTCTGAAATTATTGAAGGTCCATCAGTGTCGTAACTTGGAGAAAGTACTGTTGCCATCAAATTTGTTGCAGAAACTACCAAATTTGGAAATACTAAACTGTGAGGAAAGTAAAGTGGATCATGTGTTTGAATGTAAAGGATTTGAGCCAGATCAAACAAAACTGAGAGAGATGGAGTTGCGAGATCTAGATTTAATAAGAAGCATATGTAATGGTCCTGCTCCACGCCGAATGTTCCAGGCTCTAAAGCAATTGGTAATTCACAATTGCAACTTCCAAGGAAGTCTCTTCACATTTGATGTAGCTCAGTGTCTTTTTCAATTGGAAAGCCTTGTTGTATCCGAATGCCCTGTCTTGGAAAGAGTAATCGAAGCAAAGAGGAAAACATTGAACAACAACAAGACCGTTCTTCCACAATTGAAGAACTTATGTTTGCAATATCTTCCAATGTTGTACAAGGGAAGTGCTACTGTTGATTTTGTGTGTCCTTCATTGGAAACCTTGCATCTGAGGGACTGCCCCCACTTGTCATTTCCACCCTCCTCTGCTTCTGACTACTTCCACAGCAGGAACCCAGTCAATGAAGATTGGAGTCTGTTTCTCCGATTTAAAGTAAAGGCTTCAGTATTTCGAAGAAAAAACATGAAGAAAGCAAATCTGAGTGAGAGGGCGCGGCCTCGCTCCAGAGGGAGAGAACACATCAAGAAAGCAAATCTGAGTGAGAGGGCGCGGCCTCTCTGA

Protein Analysis

1231

Amino Acids

139.96

Weight (kDa)

7.21

Isoelectric Point (pI)

45.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 102 - 274 2e-45 TIR domain
TIR_2 PF13676 105 - 205 1.8e-12 TIR domain
NB-ARC PF00931 293 - 460 1.1e-30 NB-ARC domain
LRR_8 PF13855 664 - 723 3.8e-10 Leucine rich repeat
LRR_14 PF23598 669 - 832 2e-15 Leucine-rich repeat region
LRR_RPS2 PF23247 960 - 1101 8e-21 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1040 - 1170 4.8e-14 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 600
AasI GACNNNNNNGTC 2 cut(s) 251, 2681
AatII GACGTC 1 cut(s) 249
Acc36I ACCTGC 1 cut(s) 627
AccI GTMKAC 3 cut(s) 336, 936, 1438
AccII CGCG 2 cut(s) 3628, 3685
AccIII TCCGGA 1 cut(s) 2671
AciI CCGC 7 cut(s) 62, 1367, 1782, 1867, 2574, 3628, 3685
AclWI GGATC 9 cut(s) 115, 128, 287, 1156, 1169, 2091, 2622, 2953, 3081
AcoI YGGCCR 2 cut(s) 1861, 1904
AcuI CTGAAG 4 cut(s) 310, 1875, 2933, 3561
AcyI GRCGYC 1 cut(s) 246
AfaI GTAC 6 cut(s) 621, 987, 2381, 2647, 3003, 3410
AfiI CCNNNNNNNGG 4 cut(s) 130, 165, 2280, 3310
AflII CTTAAG 1 cut(s) 98
AflIII ACRYGT 2 cut(s) 976, 2840
AjnI CCWGG 3 cut(s) 1157, 2941, 3192
AjuI GAANNNNNNNTTGG 2 cut(s) 2427, 2459
AloI GAACNNNNNNTCC 2 cut(s) 2772, 2804
Alw26I GTCTC 3 cut(s) 259, 555, 3245
AlwI GGATC 9 cut(s) 115, 128, 287, 1156, 1169, 2091, 2622, 2953, 3081
Ama87I CYCGRG 1 cut(s) 1725
Aor13HI TCCGGA 1 cut(s) 2671
AoxI GGCC 6 cut(s) 918, 1467, 1861, 1904, 3629, 3686
ApaI GGGCCC 1 cut(s) 1471
ApeKI GCWGC 7 cut(s) 376, 866, 1505, 1629, 2300, 2303, 2571
ArsI GACNNNNNNTTYG 2 cut(s) 3291, 3323
AseI ATTAAT 1 cut(s) 1838
Asp700I GAANNNNTTC 1 cut(s) 882
AspLEI GCGC 5 cut(s) 904, 1350, 1433, 3628, 3685
AspS9I GGNCC 5 cut(s) 1467, 1468, 1753, 2974, 3170
AsuC2I CCSGG 1 cut(s) 739
AsuHPI GGTGA 6 cut(s) 145, 706, 926, 982, 1888, 2942
AsuII TTCGAA 1 cut(s) 3586
AvaI CYCGRG 1 cut(s) 1725
AvaII GGWCC 3 cut(s) 1753, 2974, 3170
BaeGI GKGCMC 1 cut(s) 1471
BalI TGGCCA 1 cut(s) 1906
BamHI GGATCC 2 cut(s) 120, 1161
BanII GRGCYC 2 cut(s) 1126, 1471
BarI GAAGNNNNNNTAC 2 cut(s) 2534, 2566
BbsI GAAGAC 3 cut(s) 1112, 1368, 1863
BbvI GCAGC 7 cut(s) 363, 878, 1492, 1616, 2287, 2315, 2583
BccI CCATC 9 cut(s) 905, 1488, 1544, 2347, 2414, 2636, 2985, 3019, 3121
BceAI ACGGC 4 cut(s) 389, 471, 955, 1876
BcgI CGANNNNNNTGC 4 cut(s) 239, 273, 2279, 2313
BciT130I CCWGG 3 cut(s) 1159, 2943, 3194
BciVI GTATCC 2 cut(s) 1045, 3304
BcnI CCSGG 1 cut(s) 739
BcoDI GTCTC 3 cut(s) 259, 555, 3245
BfaI CTAG 6 cut(s) 380, 533, 612, 1767, 2463, 3143
BfmI CTRYAG 2 cut(s) 2301, 2953
BfoI RGCGCY 2 cut(s) 905, 1351
BfrI CTTAAG 1 cut(s) 98
BfuAI ACCTGC 1 cut(s) 627
BfuI GTATCC 2 cut(s) 1045, 3304
BglII AGATCT 1 cut(s) 3139
BmcAI AGTACT 1 cut(s) 3003
Bme1390I CCNGG 4 cut(s) 739, 1159, 2943, 3194
Bme18I GGWCC 3 cut(s) 1753, 2974, 3170
BmeT110I CYCGRG 1 cut(s) 1725
BmgT120I GGNCC 5 cut(s) 1467, 1468, 1753, 2974, 3170
BmiI GGNNCC 6 cut(s) 122, 1163, 1301, 1469, 2665, 3527
BmrFI CCNGG 4 cut(s) 739, 1159, 2943, 3194
BmrI ACTGGG 2 cut(s) 2413, 3524
BmsI GCATC 9 cut(s) 269, 616, 637, 1797, 1906, 2274, 2494, 2523, 3469
BmuI ACTGGG 2 cut(s) 2413, 3524
BpiI GAAGAC 3 cut(s) 1112, 1368, 1863
BpmI CTGGAG 2 cut(s) 1982, 3622
Bpu14I TTCGAA 1 cut(s) 3586
BpuEI CTTGAG 3 cut(s) 1082, 2231, 2653
BpuMI CCSGG 1 cut(s) 739
BsaAI YACGTR 1 cut(s) 300
BsaHI GRCGYC 1 cut(s) 246
BsaJI CCNNGG 5 cut(s) 844, 2313, 2658, 2764, 3232
BsaWI WCCGGW 1 cut(s) 2671
Bsc4I CCNNNNNNNGG 4 cut(s) 130, 165, 2280, 3310
Bse118I RCCGGY 1 cut(s) 1433
Bse1I ACTGG 8 cut(s) 13, 159, 239, 756, 2408, 2681, 2907, 3530
Bse3DI GCAATG 1 cut(s) 80
BseAI TCCGGA 1 cut(s) 2671
BseBI CCWGG 3 cut(s) 1159, 2943, 3194
BseDI CCNNGG 5 cut(s) 844, 2313, 2658, 2764, 3232
BseLI CCNNNNNNNGG 4 cut(s) 130, 165, 2280, 3310
BseMI GCAATG 1 cut(s) 80
BseMII CTCAG 7 cut(s) 2697, 2705, 3110, 3275, 3455, 3605, 3662
BseNI ACTGG 8 cut(s) 13, 159, 239, 756, 2408, 2681, 2907, 3530
BseRI GAGGAG 1 cut(s) 3487
BseSI GKGCMC 1 cut(s) 1471
BseXI GCAGC 7 cut(s) 363, 878, 1492, 1616, 2287, 2315, 2583
BseYI CCCAGC 1 cut(s) 1084
BsgI GTGCAG 1 cut(s) 1769
Bsh1236I CGCG 2 cut(s) 3628, 3685
BshFI GGCC 6 cut(s) 920, 1469, 1863, 1906, 3631, 3688
BsiHKCI CYCGRG 1 cut(s) 1725
BsiSI CCGG 3 cut(s) 738, 1434, 2672
BslFI GGGAC 3 cut(s) 826, 2414, 3482
BslI CCNNNNNNNGG 4 cut(s) 130, 165, 2280, 3310
BsmAI GTCTC 3 cut(s) 259, 555, 3245
BsmBI CGTCTC 1 cut(s) 555
BsmFI GGGAC 3 cut(s) 826, 2414, 3482
BsmI GAATGC 6 cut(s) 745, 1433, 2026, 2732, 2836, 3305
BsnI GGCC 6 cut(s) 920, 1469, 1863, 1906, 3631, 3688
BsoBI CYCGRG 1 cut(s) 1725
Bsp119I TTCGAA 1 cut(s) 3586
Bsp120I GGGCCC 1 cut(s) 1467
Bsp1286I GDGCHC 2 cut(s) 1126, 1471
Bsp13I TCCGGA 1 cut(s) 2671
Bsp1407I TGTACA 3 cut(s) 985, 2379, 3408
Bsp19I CCATGG 1 cut(s) 2658
BspACI CCGC 7 cut(s) 62, 1367, 1782, 1867, 2574, 3628, 3685
BspANI GGCC 6 cut(s) 920, 1469, 1863, 1906, 3631, 3688
BspCNI CTCAG 7 cut(s) 2698, 2706, 3111, 3274, 3456, 3606, 3663
BspEI TCCGGA 1 cut(s) 2671
BspFNI CGCG 2 cut(s) 3628, 3685
BspHI TCATGA 2 cut(s) 886, 894
BspLI GGNNCC 6 cut(s) 122, 1163, 1301, 1469, 2665, 3527
BspMAI CTGCAG 2 cut(s) 2305, 2957
BspMI ACCTGC 1 cut(s) 627
BspPI GGATC 9 cut(s) 115, 128, 287, 1156, 1169, 2091, 2622, 2953, 3081
BspQI GCTCTTC 1 cut(s) 2367
BspT104I TTCGAA 1 cut(s) 3586
BspTI CTTAAG 1 cut(s) 98
BsrDI GCAATG 1 cut(s) 80
BsrFI RCCGGY 1 cut(s) 1433
BsrGI TGTACA 3 cut(s) 985, 2379, 3408
BsrI ACTGG 8 cut(s) 13, 159, 239, 756, 2408, 2681, 2907, 3530
BssAI RCCGGY 1 cut(s) 1433
BssECI CCNNGG 5 cut(s) 844, 2313, 2658, 2764, 3232
BssNI GRCGYC 1 cut(s) 246
BssT1I CCWWGG 3 cut(s) 2313, 2658, 3232
Bst2UI CCWGG 3 cut(s) 1159, 2943, 3194
Bst4CI ACNGT 6 cut(s) 2765, 2807, 3006, 3056, 3361, 3427
Bst6I CTCTTC 7 cut(s) 173, 270, 514, 1971, 2367, 2877, 3248
BstACI GRCGYC 1 cut(s) 246
BstAFI CTTAAG 1 cut(s) 98
BstAUI TGTACA 3 cut(s) 985, 2379, 3408
BstBAI YACGTR 1 cut(s) 300
BstBI TTCGAA 1 cut(s) 3586
BstC8I GCNNGC 3 cut(s) 1772, 1865, 1967
BstDSI CCRYGG 2 cut(s) 2658, 2764
BstFNI CGCG 2 cut(s) 3628, 3685
BstH2I RGCGCY 2 cut(s) 905, 1351
BstHHI GCGC 5 cut(s) 904, 1350, 1433, 3628, 3685
BstMAI GTCTC 3 cut(s) 259, 555, 3245
BstMWI GCNNNNNNNGC 3 cut(s) 948, 1454, 2383
BstNI CCWGG 3 cut(s) 1159, 2943, 3194
BstNSI RCATGY 3 cut(s) 980, 2386, 2844
BstSCI CCNGG 4 cut(s) 737, 1157, 2941, 3192
BstSFI CTRYAG 2 cut(s) 2301, 2953
BstSLI GKGCMC 1 cut(s) 1471
BstUI CGCG 2 cut(s) 3628, 3685
BstV1I GCAGC 7 cut(s) 363, 878, 1492, 1616, 2287, 2315, 2583
BstV2I GAAGAC 3 cut(s) 1112, 1368, 1863
BstX2I RGATCY 7 cut(s) 120, 292, 1161, 2083, 2627, 2945, 3139
BstYI RGATCY 7 cut(s) 120, 292, 1161, 2083, 2627, 2945, 3139
BsuI GTATCC 2 cut(s) 1045, 3304
BsuRI GGCC 6 cut(s) 920, 1469, 1863, 1906, 3631, 3688
BtgI CCRYGG 2 cut(s) 2658, 2764
BtsI GCAGTG 1 cut(s) 707
BtsIMutI CAGTG 5 cut(s) 707, 749, 1749, 2987, 3269
BveI ACCTGC 1 cut(s) 627
Cac8I GCNNGC 3 cut(s) 1772, 1865, 1967
CciI TCATGA 2 cut(s) 886, 894
CfoI GCGC 5 cut(s) 904, 1350, 1433, 3628, 3685
Cfr10I RCCGGY 1 cut(s) 1433
Cfr13I GGNCC 5 cut(s) 1467, 1468, 1753, 2974, 3170
Csp6I GTAC 6 cut(s) 620, 986, 2380, 2646, 3002, 3409
CspCI CAANNNNNGTGG 2 cut(s) 910, 945
CviQI GTAC 6 cut(s) 620, 986, 2380, 2646, 3002, 3409
DraI TTTAAA 4 cut(s) 366, 760, 2031, 3565
DrdI GACNNNNNNGTC 2 cut(s) 251, 2681
DseDI GACNNNNNNGTC 2 cut(s) 251, 2681
EaeI YGGCCR 2 cut(s) 1861, 1904
Eam1104I CTCTTC 7 cut(s) 173, 270, 514, 1971, 2367, 2877, 3248
EarI CTCTTC 7 cut(s) 173, 270, 514, 1971, 2367, 2877, 3248
Eco130I CCWWGG 3 cut(s) 2313, 2658, 3232
Eco24I GRGCYC 2 cut(s) 1126, 1471
Eco32I GATATC 3 cut(s) 1654, 2038, 2352
Eco47I GGWCC 3 cut(s) 1753, 2974, 3170
Eco57I CTGAAG 4 cut(s) 310, 1875, 2933, 3561
Eco88I CYCGRG 1 cut(s) 1725
EcoO109I RGGNCCY 3 cut(s) 1467, 1468, 1753
EcoRI GAATTC 2 cut(s) 582, 1215
EcoRII CCWGG 3 cut(s) 1157, 2941, 3192
EcoRV GATATC 3 cut(s) 1654, 2038, 2352
EcoT14I CCWWGG 3 cut(s) 2313, 2658, 3232
EcoT22I ATGCAT 2 cut(s) 1571, 1812
EcoT38I GRGCYC 2 cut(s) 1126, 1471
ErhI CCWWGG 3 cut(s) 2313, 2658, 3232
Esp3I CGTCTC 1 cut(s) 555
FalI AAGNNNNNCTT 2 cut(s) 990, 1022
FaqI GGGAC 3 cut(s) 826, 2414, 3482
FauI CCCGC 1 cut(s) 1360
FauNDI CATATG 1 cut(s) 3161
FblI GTMKAC 3 cut(s) 336, 936, 1438
FriOI GRGCYC 2 cut(s) 1126, 1471
FspBI CTAG 6 cut(s) 380, 533, 612, 1767, 2463, 3143
GlaI GCGC 5 cut(s) 903, 1349, 1432, 3627, 3684
GsaI CCCAGC 1 cut(s) 1088
GsuI CTGGAG 2 cut(s) 1982, 3622
HaeII RGCGCY 2 cut(s) 905, 1351
HaeIII GGCC 6 cut(s) 920, 1469, 1863, 1906, 3631, 3688
HapII CCGG 3 cut(s) 738, 1434, 2672
HhaI GCGC 5 cut(s) 904, 1350, 1433, 3628, 3685
Hin1I GRCGYC 1 cut(s) 246
Hin6I GCGC 5 cut(s) 902, 1348, 1431, 3626, 3683
HinP1I GCGC 5 cut(s) 902, 1348, 1431, 3626, 3683
HincII GTYRAC 3 cut(s) 244, 1734, 2686
HindII GTYRAC 3 cut(s) 244, 1734, 2686
HindIII AAGCTT 4 cut(s) 659, 1004, 2145, 2207
HpaII CCGG 3 cut(s) 738, 1434, 2672
HphI GGTGA 6 cut(s) 145, 706, 926, 982, 1888, 2942
Hpy166II GTNNAC 9 cut(s) 244, 337, 937, 986, 1439, 1734, 2551, 2585, 2686
Hpy8I GTNNAC 9 cut(s) 244, 337, 937, 986, 1439, 1734, 2551, 2585, 2686
Hpy99I CGWCG 2 cut(s) 251, 1515
HpyCH4III ACNGT 6 cut(s) 2765, 2807, 3006, 3056, 3361, 3427
HpyCH4IV ACGT 3 cut(s) 150, 246, 299
HpyF10VI GCNNNNNNNGC 3 cut(s) 948, 1454, 2383
HpySE526I ACGT 3 cut(s) 150, 246, 299
Hsp92I GRCGYC 1 cut(s) 246
HspAI GCGC 5 cut(s) 902, 1348, 1431, 3626, 3683
Kpn2I TCCGGA 1 cut(s) 2671
LguI GCTCTTC 1 cut(s) 2367
LmnI GCTCC 3 cut(s) 1299, 3181, 3641
Lsp1109I GCAGC 7 cut(s) 363, 878, 1492, 1616, 2287, 2315, 2583
LweI GCATC 9 cut(s) 269, 616, 637, 1797, 1906, 2274, 2494, 2523, 3469
MaeI CTAG 6 cut(s) 380, 533, 612, 1767, 2463, 3143
MaeII ACGT 3 cut(s) 150, 246, 299
MaeIII GTNAC 5 cut(s) 139, 191, 2741, 2934, 2987
MfeI CAATTG 6 cut(s) 459, 1659, 3206, 3220, 3275, 3371
MflI RGATCY 7 cut(s) 120, 292, 1161, 2083, 2627, 2945, 3139
MhlI GDGCHC 2 cut(s) 1126, 1471
MlsI TGGCCA 1 cut(s) 1906
MluNI TGGCCA 1 cut(s) 1906
MlyI GAGTC 6 cut(s) 575, 942, 1739, 2085, 2644, 3556
MmeI TCCRAC 1 cut(s) 934
Mox20I TGGCCA 1 cut(s) 1906
Mph1103I ATGCAT 2 cut(s) 1571, 1812
MroI TCCGGA 1 cut(s) 2671
MroXI GAANNNNTTC 1 cut(s) 882
MscI TGGCCA 1 cut(s) 1906
MslI CAYNNNNRTG 4 cut(s) 612, 981, 2657, 2750
Msp20I TGGCCA 1 cut(s) 1906
MspA1I CMGCKG 3 cut(s) 281, 1867, 2574
MspCI CTTAAG 1 cut(s) 98
MspI CCGG 3 cut(s) 738, 1434, 2672
MspR9I CCNGG 4 cut(s) 739, 1159, 2943, 3194
MunI CAATTG 6 cut(s) 459, 1659, 3206, 3220, 3275, 3371
Mva1269I GAATGC 6 cut(s) 745, 1433, 2026, 2732, 2836, 3305
MvaI CCWGG 3 cut(s) 1159, 2943, 3194
MvnI CGCG 2 cut(s) 3628, 3685
MwoI GCNNNNNNNGC 3 cut(s) 948, 1454, 2383
NciI CCSGG 1 cut(s) 739
NcoI CCATGG 1 cut(s) 2658
NdeI CATATG 1 cut(s) 3161
NlaIV GGNNCC 6 cut(s) 122, 1163, 1301, 1469, 2665, 3527
NmuCI GTSAC 1 cut(s) 139
NsiI ATGCAT 2 cut(s) 1571, 1812
NspI RCATGY 3 cut(s) 980, 2386, 2844
NspV TTCGAA 1 cut(s) 3586
PagI TCATGA 2 cut(s) 886, 894
PciI ACATGT 2 cut(s) 976, 2840
PciSI GCTCTTC 1 cut(s) 2367
PcsI WCGNNNNNNNCGW 1 cut(s) 570
PctI GAATGC 6 cut(s) 745, 1433, 2026, 2732, 2836, 3305
PdmI GAANNNNTTC 1 cut(s) 882
PfeI GAWTC 8 cut(s) 390, 467, 683, 824, 1340, 1763, 2215, 2592
PleI GAGTC 6 cut(s) 574, 941, 1738, 2084, 2643, 3555
PpsI GAGTC 6 cut(s) 574, 941, 1738, 2084, 2643, 3555
Ppu21I YACGTR 1 cut(s) 300
PpuMI RGGWCCY 1 cut(s) 1753
PscI ACATGT 2 cut(s) 976, 2840
PshBI ATTAAT 1 cut(s) 1838
PsiI TTATAA 1 cut(s) 600
Psp5II RGGWCCY 1 cut(s) 1753
Psp6I CCWGG 3 cut(s) 1157, 2941, 3192
PspFI CCCAGC 1 cut(s) 1084
PspGI CCWGG 3 cut(s) 1157, 2941, 3192
PspN4I GGNNCC 6 cut(s) 122, 1163, 1301, 1469, 2665, 3527
PspOMI GGGCCC 1 cut(s) 1467
PspPI GGNCC 5 cut(s) 1467, 1468, 1753, 2974, 3170
PspPPI RGGWCCY 1 cut(s) 1753
PstI CTGCAG 2 cut(s) 2305, 2957
PsuI RGATCY 7 cut(s) 120, 292, 1161, 2083, 2627, 2945, 3139
PvuII CAGCTG 1 cut(s) 281
RsaI GTAC 6 cut(s) 621, 987, 2381, 2647, 3003, 3410
RsaNI GTAC 6 cut(s) 620, 986, 2380, 2646, 3002, 3409
RseI CAYNNNNRTG 4 cut(s) 612, 981, 2657, 2750
SapI GCTCTTC 1 cut(s) 2367
Sau96I GGNCC 5 cut(s) 1467, 1468, 1753, 2974, 3170
ScaI AGTACT 1 cut(s) 3003
SchI GAGTC 6 cut(s) 575, 942, 1739, 2085, 2644, 3556
ScrFI CCNGG 4 cut(s) 739, 1159, 2943, 3194
SduI GDGCHC 2 cut(s) 1126, 1471
SfaNI GCATC 9 cut(s) 269, 616, 637, 1797, 1906, 2274, 2494, 2523, 3469
SfcI CTRYAG 2 cut(s) 2301, 2953
SfuI TTCGAA 1 cut(s) 3586
SinI GGWCC 3 cut(s) 1753, 2974, 3170
SmiI ATTTAAAT 1 cut(s) 2031
SmiMI CAYNNNNRTG 4 cut(s) 612, 981, 2657, 2750
SmlI CTYRAG 4 cut(s) 98, 1097, 2210, 2632
SmoI CTYRAG 4 cut(s) 98, 1097, 2210, 2632
SsiI CCGC 7 cut(s) 62, 1367, 1782, 1867, 2574, 3628, 3685
SspI AATATT 3 cut(s) 352, 1836, 2443
SspMI CTAG 6 cut(s) 380, 533, 612, 1767, 2463, 3143
StyD4I CCNGG 4 cut(s) 737, 1157, 2941, 3192
StyI CCWWGG 3 cut(s) 2313, 2658, 3232
SwaI ATTTAAAT 1 cut(s) 2031
TaaI ACNGT 6 cut(s) 2765, 2807, 3006, 3056, 3361, 3427
TaiI ACGT 3 cut(s) 153, 249, 302
TaqI TCGA 9 cut(s) 249, 1474, 1510, 1702, 2289, 2535, 2608, 3324, 3586
TatI WGTACW 4 cut(s) 985, 2379, 3001, 3408
TauI GCSGC 3 cut(s) 2577, 3631, 3688
TfiI GAWTC 8 cut(s) 390, 467, 683, 824, 1340, 1763, 2215, 2592
TscAI CASTG 5 cut(s) 707, 756, 1749, 2987, 3269
TseFI GTSAC 1 cut(s) 139
TseI GCWGC 7 cut(s) 376, 866, 1505, 1629, 2300, 2303, 2571
Tsp45I GTSAC 1 cut(s) 139
TspGWI ACGGA 5 cut(s) 783, 1124, 1529, 1809, 2192
TspRI CASTG 5 cut(s) 707, 756, 1749, 2987, 3269
Vha464I CTTAAG 1 cut(s) 98
VpaK11BI GGWCC 3 cut(s) 1753, 2974, 3170
VspI ATTAAT 1 cut(s) 1838
XbaI TCTAGA 1 cut(s) 3142
XceI RCATGY 3 cut(s) 980, 2386, 2844
XmiI GTMKAC 3 cut(s) 336, 936, 1438
XmnI GAANNNNTTC 1 cut(s) 882
XspI CTAG 6 cut(s) 380, 533, 612, 1767, 2463, 3143
ZraI GACGTC 1 cut(s) 247
ZrmI AGTACT 1 cut(s) 3003
Zsp2I ATGCAT 2 cut(s) 1571, 1812
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.