Rw0G012980

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00577
OG0000085
Physical Location & Seq
Forward (+)
14348 .. 18187
3840 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G012980.1

Sequence Viewer

Length: 3276 bp
ATGGCCTCCAGCTCCCAAATAGCCTGTGCATCTCCTCCTTCATCAGATCCAAGTTGGAAGAATGATGTGTTTTTGAGTTTCAGGGGTGTAGACACTCGCAAGGGGATTACAGTCGACATACACGACCGACTCACTAGGAGAGGAGTTAATACGTTCCTGGATGAGCAAAACCTTAGAGTAGGCGATGCTATTTCTCCCACTCTTGTAAAGGCAATTAAAGAATCAAGGTTTGCAATTGTTGTTCTCTCGAAAAATTATGCTTCTTCTACTTGGTGTTTGGAGGAACTTAGAGAGATTTGTGAGTGCATGAAAGACCATAACAGAATTTTGCCACTATTTTATCATGTTGATCCTACTGATGTTCGATATCAGAAAAACAGTTTCGGAGACGCTTTCATTAAGCATGAAAACTCTCGGCGACACAAATCAAAGAAGATGAAACAATGGAGAGATGCTTTAAAGAAAGTGGCCGGTTTCTCGGGGTGGAATACACAAGATTATAAGACTCACAAACAACTTGTTGACGTCATTGTGGAATTTATCTGCAGTATAGTAGTACCTGATGCAATTGAGTCCACAGAAGATTTCGAAGCATTTGAAGTAACAAAACAAGCCATGTATGAGATTATGAAGGCGTTTACAGATGATGACGTCACTGCAATTGGTGTCTACGGCATGGGAGGCGTTGGAAAGACAACCATGGTAAAACATGTCGCTGCACATGCTAGAAAAAGTGGGATTTTTGATCATGTGATTATGGGTGTCGTATCCCAAAACCCTGACTTGAAAAGAATTCAAGACACATTGGCAGAGTTGCTGGGCTTCGAATTACATGAGGGGACAGAAATTGGAAGAGCAGCTAGATTGAGCAAGGAGATAAAGAGAAGAAATAAGATCCTTATAATCCTGGACGACATTTGGGAGAGAATGGAATTGTCAGACATAGGAATACCTAGCTACAAGGAACTTCGAAAGTGCAAATCCAAAGTGCTACTCACCACAAGGATAAGAAATGTGTGTCATGCGATGAAGTGCCAAGAAAAGATCAACCTCACTACCCTATCAGAAGAAGATTCTTGGACCTTGTTTGTGAGAAATGCAGGAAGGTCTTTTGAATCCACCAATTTCGAGGATGTTGCGAGGAGGGTAGCTGGAGAGTGTTGCGGTTTACCGATTGCATTGATAGCAGTTGCAAGGGCACTCGGAGATAAAGACTTGGTGGAATGGAAGAAAGCAGCTCAAAGATTAGAGAAGTCTCATGCTGCCAACCCTGAGGATTACGGAGAGGCATCCAAGTGTATAAAATTAAGCTATGATTACTTGAAAGAGGAAGACTATAAGTCATACTTCTTGCTCTGTTGCTTATTCCCAGAAGACTTTGAAATCGAAATAGAAGACTTGTTCAAGTATGCGATCGGGAAAGGGTTGTTTCGAGATGCCGACACAATCGAAGAAGCCAGAGCCGAAGCTGATTCCGTGGCCACACACCTTAAACATTGTTGCTTGCTTTTGGATGGTGAAAACAATAAATTTGTAAGGATGCATGATGTTGTCCGGGATACAGCCATTCAAATTGCGCAATCTGAAGATGGGCATGGGTTTTTCGTGAAAGCTGGCTGTGGTTTAAAGGATTGGCCTCCTCGATTACATGAAGGCTATTCGGCAATCTCACTAATGGCTAATTGTATTGCCAAGCTACCTGAAAAGTTGGTATGTCCAAAACTCCAAATTTTGTTACTCAACTCTAATTCCTCTATAGGAGAGATCCCAGAATCCTTTTTTCAAAGTCCGAATGAATTAACGGTCTTAGATCTTACGCGGACCCATATTTCATTACTACCCCAATCATTCAGTCTGCTAACCAATCTCCAAGCTTTGTATTTAGATCGTTGTTTCAAATTTATTGACACTTCTAAACTGGGAGAACTTAAGAAGCTTGAGATTCTTAGTATGAGAAATTCATGTTTTAAGGGATTGTCGAGAGAAATAGGAGATTTGACCAATCTAAGGATGTTGGATATCACCAGTAAAAGTATTGACACAGTTCCATCCAAAGTGATATCAAAGTTGCATAATTTAGAAGAACTGTACATGCAGGGATTTTGGAACTGGGGGAGTAAAGTTGTGGGAGAAGGAGACGAAACCAATGCGGGCTTTGACGAGGTAACTAGCTTGTCAAATTTAAGAGTTTTGGAGGTTTGTATCCCCGATGAAGAATGTATCCCTAAAGATGTTGAGTTCAATCCGAATTGGGTTAAGTTTAATATATGTATCGGCAGAGACGACAAGATGAAAGCCACTGATCAATATGCTCATAATTCAATATTCTTGAAGATTGACACTATCAGTAACTTACCAGACTGGTTTTGCAATGCGGTGATGACCATAGCAGGAAGCCTACAGTGGAGAGGGTGCCAAAGGCTTATTGAATGTTTTATGGAATATGAGCATGGAAGGTTACATGGATTGAAGCATCTCTCTGTATATGGTGACTATGACCACGAGCTGGTGAACGTGTTAACATGGGTTCCGAAAGAACCTGTGTTTGAGAACTTGGAAGAGCTGCGTCTGATTGGCGTAGATTGCCGGGAATTATGTGTTGGTGAATTACCACCTGGGTCTTTGTTCAATTTGAAGTTATTAGAGGTAAATTGTTACAACTGGGGGATCAATGTACTTTTACCATCAACATTGTTACAGAGACTTCCAAATCTGGAAGAACTAAATTGTTGTGGCATGGAGGAGATTGAATATGTGTTTGGGTATGGAGCGTTATTTGAGCCAAAACACTCAAAATTGAGAAAGATAACGTTGTCTCGTCTACCGACAGTAAGAAGCATATCTAATGCACCTGCTCCACCTGCAATGTTCCAGAATCTTGAGTGGTTGTTCATTGATTATTGCCACTTGCGGGGAAGTCTCTTCACCTCTGATGTTGCTCAATGTCTTTTTCAGTTGAAATACATTGATATACGGTATTGCCCCCTTCTGGAAAGAATAGTTGAAGTAAGCAACAAGAAGATCATTCTTCCAAATTTGGAGGAATTAGCTTTGTTCCTACTTGATATGATCTGCTATGAAAGTGCTACTTTTGATATTGAGTGTCCTTCATTGGAAAGATTGTGTGTGTTTGGCTGCCCGAAATTTTTAGCCTCTTATTCTGACTTCCACAGCAGGAAAGAAATCGAACTCAACAGGTATTCAATTTATTTATGGATGCGGCTATTGTCACCCAACCATTTTCTTAGTTCACCCTACAAATACATGAATTATTGA

Protein Analysis

1091

Amino Acids

124.58

Weight (kDa)

5.82

Isoelectric Point (pI)

42.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 22 - 181 2.7e-44 TIR domain
TIR_2 PF13676 23 - 118 1.4e-16 TIR domain
NB-ARC PF00931 205 - 367 4.8e-31 NB-ARC domain
LRR_8 PF13855 573 - 630 3.4e-09 Leucine rich repeat
LRR_14 PF23598 583 - 703 2.4e-13 Leucine-rich repeat region
LRR_RPS2 PF23247 852 - 994 7.5e-17 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 501, 902
AarI CACCTGC 2 cut(s) 2860, 2869
AatII GACGTC 2 cut(s) 528, 654
Acc16I TGCGCA 1 cut(s) 1578
Acc36I ACCTGC 2 cut(s) 2860, 2869
AccB1I GGYRCC 1 cut(s) 2412
AccB7I CCANNNNNTGG 1 cut(s) 2506
AccI GTMKAC 4 cut(s) 90, 114, 669, 2821
AccII CGCG 1 cut(s) 1819
AciI CCGC 6 cut(s) 1164, 1819, 2150, 2375, 2911, 3220
AclI AACGTT 1 cut(s) 2810
AclWI GGATC 5 cut(s) 41, 344, 889, 1759, 2675
AcoI YGGCCR 2 cut(s) 468, 1479
AcuI CTGAAG 1 cut(s) 1605
AcyI GRCGYC 2 cut(s) 525, 651
AfaI GTAC 3 cut(s) 558, 2090, 2676
AfiI CCNNNNNNNGG 4 cut(s) 2007, 2506, 2911, 2989
AflII CTTAAG 1 cut(s) 1928
AflIII ACRYGT 2 cut(s) 709, 2514
AhdI GACNNNNNGTC 1 cut(s) 1339
AjnI CCWGG 3 cut(s) 156, 906, 2614
AjuI GAANNNNNNNTTGG 4 cut(s) 2583, 2615, 2742, 2774
Alw26I GTCTC 7 cut(s) 381, 1260, 2130, 2274, 2695, 2820, 2924
AlwI GGATC 5 cut(s) 41, 344, 889, 1759, 2675
Ama87I CYCGRG 1 cut(s) 478
AoxI GGCC 4 cut(s) 3, 468, 1479, 1634
ApeKI GCWGC 6 cut(s) 716, 857, 1235, 1262, 2563, 3135
AspLEI GCGC 1 cut(s) 1579
AspS9I GGNCC 2 cut(s) 1080, 1821
AsuC2I CCSGG 2 cut(s) 1556, 2588
AsuII TTCGAA 3 cut(s) 588, 825, 970
AvaI CYCGRG 1 cut(s) 478
AvaII GGWCC 2 cut(s) 1080, 1821
AxyI CCTNAGG 1 cut(s) 1272
BaeGI GKGCMC 1 cut(s) 1201
BalI TGGCCA 1 cut(s) 1481
BanI GGYRCC 1 cut(s) 2412
BauI CACGAG 1 cut(s) 2501
BbsI GAAGAC 3 cut(s) 1338, 1380, 1401
BbvI GCAGC 6 cut(s) 703, 869, 1247, 1249, 2550, 3122
BccI CCATC 4 cut(s) 1508, 1583, 2056, 2692
BceAI ACGGC 1 cut(s) 688
BcgI CGANNNNNNTGC 4 cut(s) 1118, 1152, 2129, 2163
BciT130I CCWGG 3 cut(s) 158, 908, 2616
BciVI GTATCC 4 cut(s) 778, 1552, 2213, 2231
BclI TGATCA 2 cut(s) 745, 2302
BcnI CCSGG 2 cut(s) 1556, 2588
BcoDI GTCTC 7 cut(s) 381, 1260, 2130, 2274, 2695, 2820, 2924
BfaI CTAG 5 cut(s) 135, 726, 861, 954, 2169
BfmI CTRYAG 3 cut(s) 544, 1755, 2399
BfrI CTTAAG 1 cut(s) 1928
BfuAI ACCTGC 2 cut(s) 2860, 2869
BfuI GTATCC 4 cut(s) 778, 1552, 2213, 2231
BglII AGATCT 1 cut(s) 1810
BisI GCNGC 7 cut(s) 717, 858, 1236, 1263, 2564, 3136, 3221
BlsI GCNGC 7 cut(s) 718, 859, 1237, 1264, 2565, 3137, 3222
Bme1390I CCNGG 5 cut(s) 158, 908, 1556, 2588, 2616
Bme18I GGWCC 2 cut(s) 1080, 1821
BmeRI GACNNNNNGTC 1 cut(s) 1339
BmeT110I CYCGRG 1 cut(s) 478
BmgT120I GGNCC 2 cut(s) 1080, 1821
BmiI GGNNCC 3 cut(s) 1823, 2414, 2529
BmrFI CCNGG 5 cut(s) 158, 908, 1556, 2588, 2616
BmrI ACTGGG 3 cut(s) 1928, 2119, 2671
BmsI GCATC 9 cut(s) 38, 175, 442, 553, 1298, 1426, 1530, 2482, 3207
BmuI ACTGGG 3 cut(s) 1928, 2119, 2671
BpiI GAAGAC 3 cut(s) 1338, 1380, 1401
BpmI CTGGAG 1 cut(s) 1173
Bpu14I TTCGAA 3 cut(s) 588, 825, 970
BpuEI CTTGAG 2 cut(s) 1958, 2900
BpuMI CCSGG 2 cut(s) 1556, 2588
BsaHI GRCGYC 2 cut(s) 525, 651
BsaJI CCNNGG 3 cut(s) 699, 1476, 2615
Bsc4I CCNNNNNNNGG 4 cut(s) 2007, 2506, 2911, 2989
Bse118I RCCGGY 1 cut(s) 470
Bse1I ACTGG 5 cut(s) 1923, 2025, 2114, 2366, 2666
Bse21I CCTNAGG 1 cut(s) 1272
Bse3DI GCAATG 2 cut(s) 2377, 2871
BseBI CCWGG 3 cut(s) 158, 908, 2616
BseDI CCNNGG 3 cut(s) 699, 1476, 2615
BseGI GGATG 8 cut(s) 166, 1138, 1289, 1519, 1545, 2016, 2048, 3222
BseLI CCNNNNNNNGG 4 cut(s) 2007, 2506, 2911, 2989
BseMI GCAATG 2 cut(s) 2377, 2871
BseMII CTCAG 1 cut(s) 1263
BseNI ACTGG 5 cut(s) 1923, 2025, 2114, 2366, 2666
BseRI GAGGAG 5 cut(s) 24, 156, 1156, 1629, 2756
BseSI GKGCMC 1 cut(s) 1201
BseXI GCAGC 6 cut(s) 703, 869, 1247, 1249, 2550, 3122
BseYI CCCAGC 1 cut(s) 817
BsgI GTGCAG 1 cut(s) 702
Bsh1236I CGCG 1 cut(s) 1819
Bsh1285I CGRYCG 2 cut(s) 127, 1416
BshFI GGCC 4 cut(s) 5, 470, 1481, 1636
BshNI GGYRCC 1 cut(s) 2412
BsiEI CGRYCG 2 cut(s) 127, 1416
BsiHKCI CYCGRG 1 cut(s) 478
BsiSI CCGG 3 cut(s) 471, 1555, 2587
BslFI GGGAC 1 cut(s) 853
BslI CCNNNNNNNGG 4 cut(s) 2007, 2506, 2911, 2989
BsmAI GTCTC 7 cut(s) 381, 1260, 2130, 2274, 2695, 2820, 2924
BsmBI CGTCTC 3 cut(s) 381, 2130, 2274
BsmFI GGGAC 1 cut(s) 853
BsnI GGCC 4 cut(s) 5, 470, 1481, 1636
BsoBI CYCGRG 1 cut(s) 478
Bsp119I TTCGAA 3 cut(s) 588, 825, 970
Bsp1286I GDGCHC 1 cut(s) 1201
Bsp1407I TGTACA 1 cut(s) 2088
Bsp19I CCATGG 1 cut(s) 699
BspACI CCGC 6 cut(s) 1164, 1819, 2150, 2375, 2911, 3220
BspANI GGCC 4 cut(s) 5, 470, 1481, 1636
BspCNI CTCAG 1 cut(s) 1264
BspFNI CGCG 1 cut(s) 1819
BspLI GGNNCC 3 cut(s) 1823, 2414, 2529
BspMAI CTGCAG 1 cut(s) 548
BspMI ACCTGC 2 cut(s) 2860, 2869
BspPI GGATC 5 cut(s) 41, 344, 889, 1759, 2675
BspQI GCTCTTC 2 cut(s) 847, 2553
BspT104I TTCGAA 3 cut(s) 588, 825, 970
BspT107I GGYRCC 1 cut(s) 2412
BspTI CTTAAG 1 cut(s) 1928
BsrDI GCAATG 2 cut(s) 2377, 2871
BsrFI RCCGGY 1 cut(s) 470
BsrGI TGTACA 1 cut(s) 2088
BsrI ACTGG 5 cut(s) 1923, 2025, 2114, 2366, 2666
BssAI RCCGGY 1 cut(s) 470
BssECI CCNNGG 3 cut(s) 699, 1476, 2615
BssNI GRCGYC 2 cut(s) 525, 651
BssSI CACGAG 1 cut(s) 2501
BssT1I CCWWGG 1 cut(s) 699
Bst2BI CACGAG 1 cut(s) 2501
Bst2UI CCWGG 3 cut(s) 158, 908, 2616
Bst4CI ACNGT 8 cut(s) 112, 380, 1804, 2044, 2088, 2403, 2830, 2976
Bst6I CTCTTC 3 cut(s) 847, 2553, 2927
BstACI GRCGYC 2 cut(s) 525, 651
BstAFI CTTAAG 1 cut(s) 1928
BstAUI TGTACA 1 cut(s) 2088
BstBI TTCGAA 3 cut(s) 588, 825, 970
BstC8I GCNNGC 3 cut(s) 1505, 1615, 2152
BstDEI CTNAG 7 cut(s) 173, 287, 1272, 1807, 1946, 2006, 3245
BstDSI CCRYGG 2 cut(s) 699, 1476
BstF5I GGATG 8 cut(s) 166, 1138, 1289, 1519, 1545, 2016, 2048, 3222
BstFNI CGCG 1 cut(s) 1819
BstHHI GCGC 1 cut(s) 1579
BstMAI GTCTC 7 cut(s) 381, 1260, 2130, 2274, 2695, 2820, 2924
BstMCI CGRYCG 2 cut(s) 127, 1416
BstMWI GCNNNNNNNGC 5 cut(s) 681, 722, 1184, 2583, 2861
BstNI CCWGG 3 cut(s) 158, 908, 2616
BstNSI RCATGY 3 cut(s) 713, 725, 2095
BstSCI CCNGG 5 cut(s) 156, 906, 1554, 2586, 2614
BstSFI CTRYAG 3 cut(s) 544, 1755, 2399
BstSLI GKGCMC 1 cut(s) 1201
BstUI CGCG 1 cut(s) 1819
BstV1I GCAGC 6 cut(s) 703, 869, 1247, 1249, 2550, 3122
BstV2I GAAGAC 3 cut(s) 1338, 1380, 1401
BstX2I RGATCY 4 cut(s) 46, 894, 1764, 1810
BstYI RGATCY 4 cut(s) 46, 894, 1764, 1810
Bsu36I CCTNAGG 1 cut(s) 1272
BsuI GTATCC 4 cut(s) 778, 1552, 2213, 2231
BsuRI GGCC 4 cut(s) 5, 470, 1481, 1636
BtgI CCRYGG 2 cut(s) 699, 1476
BtgZI GCGATG 2 cut(s) 198, 1040
BtsCI GGATG 8 cut(s) 166, 1138, 1289, 1519, 1545, 2016, 2048, 3222
BtsI GCAGTG 1 cut(s) 654
BtsIMutI CAGTG 3 cut(s) 654, 2298, 2408
BveI ACCTGC 2 cut(s) 2860, 2869
Cac8I GCNNGC 3 cut(s) 1505, 1615, 2152
CfoI GCGC 1 cut(s) 1579
Cfr10I RCCGGY 1 cut(s) 470
Cfr13I GGNCC 2 cut(s) 1080, 1821
CseI GACGC 2 cut(s) 398, 2555
Csp6I GTAC 3 cut(s) 557, 2089, 2675
CviQI GTAC 3 cut(s) 557, 2089, 2675
DdeI CTNAG 7 cut(s) 173, 287, 1272, 1807, 1946, 2006, 3245
DraI TTTAAA 2 cut(s) 459, 1626
DriI GACNNNNNGTC 1 cut(s) 1339
EaeI YGGCCR 2 cut(s) 468, 1479
Eam1104I CTCTTC 3 cut(s) 847, 2553, 2927
Eam1105I GACNNNNNGTC 1 cut(s) 1339
EarI CTCTTC 3 cut(s) 847, 2553, 2927
Eco130I CCWWGG 1 cut(s) 699
Eco32I GATATC 3 cut(s) 368, 2020, 2061
Eco47I GGWCC 2 cut(s) 1080, 1821
Eco57I CTGAAG 1 cut(s) 1605
Eco81I CCTNAGG 1 cut(s) 1272
Eco88I CYCGRG 1 cut(s) 478
EcoRI GAATTC 1 cut(s) 792
EcoRII CCWGG 3 cut(s) 156, 906, 2614
EcoRV GATATC 3 cut(s) 368, 2020, 2061
EcoT14I CCWWGG 1 cut(s) 699
EcoT22I ATGCAT 1 cut(s) 1545
ErhI CCWWGG 1 cut(s) 699
Esp3I CGTCTC 3 cut(s) 381, 2130, 2274
FalI AAGNNNNNCTT 4 cut(s) 1331, 1363, 3073, 3105
FaqI GGGAC 1 cut(s) 853
FauI CCCGC 2 cut(s) 2143, 2904
FbaI TGATCA 2 cut(s) 745, 2302
FblI GTMKAC 4 cut(s) 90, 114, 669, 2821
Fnu4HI GCNGC 7 cut(s) 717, 858, 1236, 1263, 2564, 3136, 3221
FokI GGATG 8 cut(s) 173, 1145, 1276, 1526, 1552, 2023, 2035, 3229
Fsp4HI GCNGC 7 cut(s) 717, 858, 1236, 1263, 2564, 3136, 3221
FspBI CTAG 5 cut(s) 135, 726, 861, 954, 2169
FspI TGCGCA 1 cut(s) 1578
GlaI GCGC 1 cut(s) 1578
GluI GCNGC 7 cut(s) 717, 858, 1236, 1263, 2564, 3136, 3221
GsaI CCCAGC 1 cut(s) 821
GsuI CTGGAG 1 cut(s) 1173
HaeIII GGCC 4 cut(s) 5, 470, 1481, 1636
HapII CCGG 3 cut(s) 471, 1555, 2587
HgaI GACGC 2 cut(s) 398, 2555
HhaI GCGC 1 cut(s) 1579
Hin1I GRCGYC 2 cut(s) 525, 651
Hin6I GCGC 1 cut(s) 1577
HinP1I GCGC 1 cut(s) 1577
HincII GTYRAC 3 cut(s) 115, 523, 2520
HindII GTYRAC 3 cut(s) 115, 523, 2520
HindIII AAGCTT 2 cut(s) 1872, 1934
HpaI GTTAAC 1 cut(s) 2520
HpaII CCGG 3 cut(s) 471, 1555, 2587
HpyAV CCTTC 8 cut(s) 48, 625, 1098, 1646, 2126, 2448, 2996, 3117
HpyCH4III ACNGT 8 cut(s) 112, 380, 1804, 2044, 2088, 2403, 2830, 2976
HpyCH4IV ACGT 5 cut(s) 152, 525, 651, 2514, 2810
HpyF10VI GCNNNNNNNGC 5 cut(s) 681, 722, 1184, 2583, 2861
HpyF3I CTNAG 7 cut(s) 173, 287, 1272, 1807, 1946, 2006, 3245
HpySE526I ACGT 5 cut(s) 152, 525, 651, 2514, 2810
Hsp92I GRCGYC 2 cut(s) 525, 651
HspAI GCGC 1 cut(s) 1577
Ksp22I TGATCA 2 cut(s) 745, 2302
KspAI GTTAAC 1 cut(s) 2520
LguI GCTCTTC 2 cut(s) 847, 2553
LmnI GCTCC 3 cut(s) 17, 2768, 2860
Lsp1109I GCAGC 6 cut(s) 703, 869, 1247, 1249, 2550, 3122
LweI GCATC 9 cut(s) 38, 175, 442, 553, 1298, 1426, 1530, 2482, 3207
MaeI CTAG 5 cut(s) 135, 726, 861, 954, 2169
MaeII ACGT 5 cut(s) 152, 525, 651, 2514, 2810
MfeI CAATTG 3 cut(s) 234, 567, 660
MflI RGATCY 4 cut(s) 46, 894, 1764, 1810
MhlI GDGCHC 1 cut(s) 1201
MlsI TGGCCA 1 cut(s) 1481
MluNI TGGCCA 1 cut(s) 1481
MlyI GAGTC 3 cut(s) 123, 499, 581
MmeI TCCRAC 3 cut(s) 35, 667, 1995
Mox20I TGGCCA 1 cut(s) 1481
Mph1103I ATGCAT 1 cut(s) 1545
MscI TGGCCA 1 cut(s) 1481
MslI CAYNNNNRTG 1 cut(s) 1294
Msp20I TGGCCA 1 cut(s) 1481
MspCI CTTAAG 1 cut(s) 1928
MspI CCGG 3 cut(s) 471, 1555, 2587
MspR9I CCNGG 5 cut(s) 158, 908, 1556, 2588, 2616
MunI CAATTG 3 cut(s) 234, 567, 660
MvaI CCWGG 3 cut(s) 158, 908, 2616
MvnI CGCG 1 cut(s) 1819
MwoI GCNNNNNNNGC 5 cut(s) 681, 722, 1184, 2583, 2861
NciI CCSGG 2 cut(s) 1556, 2588
NcoI CCATGG 1 cut(s) 699
NlaIV GGNNCC 3 cut(s) 1823, 2414, 2529
NmeAIII GCCGAG 1 cut(s) 394
NmuCI GTSAC 3 cut(s) 652, 2489, 3228
NsbI TGCGCA 1 cut(s) 1578
NsiI ATGCAT 1 cut(s) 1545
NspI RCATGY 3 cut(s) 713, 725, 2095
NspV TTCGAA 3 cut(s) 588, 825, 970
PaqCI CACCTGC 2 cut(s) 2860, 2869
PciI ACATGT 1 cut(s) 709
PciSI GCTCTTC 2 cut(s) 847, 2553
PcsI WCGNNNNNNNCGW 2 cut(s) 120, 2280
PfeI GAWTC 7 cut(s) 221, 1073, 1115, 1472, 1772, 1942, 2875
PflMI CCANNNNNTGG 1 cut(s) 2506
PfoI TCCNGGA 3 cut(s) 156, 906, 1554
PkrI GCNGC 7 cut(s) 718, 859, 1237, 1264, 2565, 3137, 3222
Ple19I CGATCG 1 cut(s) 1416
PleI GAGTC 3 cut(s) 123, 499, 580
PpsI GAGTC 3 cut(s) 123, 499, 580
PscI ACATGT 1 cut(s) 709
PsiI TTATAA 2 cut(s) 501, 902
Psp1406I AACGTT 1 cut(s) 2810
Psp6I CCWGG 3 cut(s) 156, 906, 2614
PspFI CCCAGC 1 cut(s) 817
PspGI CCWGG 3 cut(s) 156, 906, 2614
PspN4I GGNNCC 3 cut(s) 1823, 2414, 2529
PspPI GGNCC 2 cut(s) 1080, 1821
PstI CTGCAG 1 cut(s) 548
PsuI RGATCY 4 cut(s) 46, 894, 1764, 1810
PvuI CGATCG 1 cut(s) 1416
RsaI GTAC 3 cut(s) 558, 2090, 2676
RsaNI GTAC 3 cut(s) 557, 2089, 2675
RseI CAYNNNNRTG 1 cut(s) 1294
SalI GTCGAC 1 cut(s) 113
SapI GCTCTTC 2 cut(s) 847, 2553
SatI GCNGC 7 cut(s) 717, 858, 1236, 1263, 2564, 3136, 3221
Sau96I GGNCC 2 cut(s) 1080, 1821
SchI GAGTC 3 cut(s) 123, 499, 581
ScrFI CCNGG 5 cut(s) 158, 908, 1556, 2588, 2616
SduI GDGCHC 1 cut(s) 1201
SfaNI GCATC 9 cut(s) 38, 175, 442, 553, 1298, 1426, 1530, 2482, 3207
SfcI CTRYAG 3 cut(s) 544, 1755, 2399
SfuI TTCGAA 3 cut(s) 588, 825, 970
SinI GGWCC 2 cut(s) 1080, 1821
SmiMI CAYNNNNRTG 1 cut(s) 1294
SmlI CTYRAG 3 cut(s) 1928, 1937, 2879
SmoI CTYRAG 3 cut(s) 1928, 1937, 2879
SsiI CCGC 6 cut(s) 1164, 1819, 2150, 2375, 2911, 3220
SspI AATATT 1 cut(s) 2325
SspMI CTAG 5 cut(s) 135, 726, 861, 954, 2169
StyD4I CCNGG 5 cut(s) 156, 906, 1554, 2586, 2614
StyI CCWWGG 1 cut(s) 699
TaaI ACNGT 8 cut(s) 112, 380, 1804, 2044, 2088, 2403, 2830, 2976
TaiI ACGT 5 cut(s) 155, 528, 654, 2517, 2813
TaqII GACCGA 1 cut(s) 141
TatI WGTACW 2 cut(s) 2088, 2674
TauI GCSGC 1 cut(s) 3223
TfiI GAWTC 7 cut(s) 221, 1073, 1115, 1472, 1772, 1942, 2875
TscAI CASTG 3 cut(s) 661, 2305, 2408
TseFI GTSAC 3 cut(s) 652, 2489, 3228
TseI GCWGC 6 cut(s) 716, 857, 1235, 1262, 2563, 3135
Tsp45I GTSAC 3 cut(s) 652, 2489, 3228
TspGWI ACGGA 2 cut(s) 1296, 1465
TspRI CASTG 3 cut(s) 661, 2305, 2408
Van91I CCANNNNNTGG 1 cut(s) 2506
Vha464I CTTAAG 1 cut(s) 1928
VpaK11BI GGWCC 2 cut(s) 1080, 1821
XceI RCATGY 3 cut(s) 713, 725, 2095
XmiI GTMKAC 4 cut(s) 90, 114, 669, 2821
XspI CTAG 5 cut(s) 135, 726, 861, 954, 2169
ZraI GACGTC 2 cut(s) 526, 652
Zsp2I ATGCAT 1 cut(s) 1545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.