Rw3G023030

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000085
Physical Location & Seq
Reverse (-)
28673335 .. 28677444
4110 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G023030.1

Sequence Viewer

Length: 3435 bp
ATGGCCTCGAGCTCCCAAACAACCTCTTCATCTCTTCCTTCGTCATCAGCTCCTCGGTGGAAGCATGATGTGTTTCTCAGCTTCAGGGGTGAAGACACCCGGAAGGGTATTGTATCCTCTTTATACCATGAATTGAAAACCAGAGGAATACAAACATTCATGGATGACCAAGGGCTTCAGCAAGGGATGCCTATTTCTCCAAGTCTCCTAACAGCGATTGAGGAATCCAGGTGTGCAGTCGTTCTTCTATCGCCAAACTATGCTTCTTCTACATGGTGTTTGGATGAACTTGCAAAGATTCTTCAATGCTCTGAAGCCAACAAGACACTGGTTCTGCCAATTTTTTATAATGTCTCCCCCTCTCATGTGCGACATCAAAAGGGAAGTTTTGCAGAAGCCTTCACTAAGCATGAAGAGAGGTATAGGCAAGGCAATGATGATGCAGAGAAGGTGAAGAAGTGGAGAGCTGCTTTAACACAAGTGGCCAATCTCTCTGGGTTGGGTTCAAACAAATTTGGGTCTGACAGAGAACTCGTCCAACGCATTGTGGAACTTGTGCGCCGTGATGTAAGGCCTATTTCAATAATGTCGAGCAGCAATTTTGTAGCATTTGAATCAACAAGAAAAGCCATGAATGAGCTGATGGAGGCATTAGAAGATGATGAGGTCGCTGCCATTGGGGTCTATGGAATGGGAGGGGTTGGGAAGACAACCATGGTGAAACATGTCGGTGCACAAGCCCAAAAAATGGGGCTTTTTGATCGTGTGATTATGGCTGTTGTATCCCAAACCCCAGATACAAGAAATATTCAAGGCGCACTTGCGGATCTGCTGGGCTTGAAATTGGTGGAGGAGTCAGAAAGTGGACGAGCCGATAGATTGCAGACGGAAATACTTGGAAGAAATAAGATCCTTATAATCTTAGATGACATTTGGAATACATTTGACTTGTCAAGCATTGGAATTCCCAGCTACAACGAGCTTCAAATCTGCAATTCCAAACTGCTATTCACCACAAGGAGATCGAATGTTTGCCATACCATGGAGAGCCAAGCAAAAATTCATGTCAATATCTTATCAGAAGATGATGCTTGGAACTTGTTTTTGAAGAAGGCAAGAAGGTCTATTCAAAAGTCTACCCCTTTCTATGAAGTAGCCAGAAAGGTAGCTAGAGAATGTGCTGGTCTCCCAGTTGCATTGATTGCAGTTGCGAGGGCACTTGGAGATAAAGATTTTGAAGACTGGAAACTAGCAGCTCGACAACTTGAGATGTCTCAACCTGCCAACCTTGACGAGGAGAAAGATGTGTTCAAGTGCATTAAGTTGAGCTATGATTACCTTGACAGTGTTGACGCCAAGTCTTGCTTCTTGCTTTGCTGCCTATTCCCAGAAGATTATGACATCCGTGTTGAAGACTTGATGAGCTATGGGTTCGGAACAGGGTTGTTTCGACATGTCAACAACACAATAGAATATGCCAGAGTCAGAGCACATTCGGTTACTAGATACCTTAAAGCTTCTTGTCTGCTTTTGGATGGGACAAAAGAAGGGTATGTAAGGATGCATGATGTCATTCGAGATATGGCCATATCTATTGCATTCTCTGTAGATGGCCACGCCTCTTTGGTGAAAACTAATTGTCATTTGAAAGATTGGTCGATGTATGTGGACGAAGGCTACTCAGCAATCTCACTAATCGAGAGCAACATTTGCAAGCTTCCCAACAAGTTGGTATGTTCAAAACTTCAGATTTTATTGCTACAAAAAAATGCTGCTATAAGAGAGATCCCAATAACCTTTTTTCAAAGCCCCAATGATTTAAGGGTCTTAGATCTTAGCAACACTGGCATTTCATCATTGCCCCCTTCACTCAGTCTCCTAACCAGACTCCAAGCTTTGCATTTAGATGATTGCAAGTCCATGATTGACATATCCATGCTTGGAAAACTGAAAAAGCTTGAGATTCTTAGTATGAGACGCTGTTACTCTATTAAGAAATTTCCAAAAGAGATTGGAAATTTGACGAATTTAAGAATGTTGGACTTGACTGCAGCCCGTTTTGAGACAGCTCCCTCTCAAGTGATATCAAGGTTGTATAGATTGGAAGTACTGTACATGAGATGTTATTTTGGCGTGTGGGGTTGTAAAGCTGCGGGAGGCTGCTTTTGTTTTCTTTCTAGTGCTAGCTTTGATGAGTTAATTGGCTTGTCAAGTTTAAACACTTTGAAGCTTTTCGTATGTGGTGCAGAATGCTTGCCTCAAAACGTTGAGTTCAATCCAAATTGGGACAGGTTTTGTATAATTATCCAAAGATACTACGAGGACTTTTTGTTCCTTTATGACGAGGCCTTCCCACATCGTTCTTCAAGAACATTGATCATGGTCGAAACCATGAATAACTTACCCGATTGGTTCATCAGTGTAGTGACAGAAAGAACAGATGATTTATGGTACAGAGGTCGCCATGGCTTAACTAACCTTCTCGAGGAACACGACCGTGGGAGATTACATGGACTCAAGTATTTGTGTATAGAATCTTCCGAATATGCCAAAGTGTTGTTGAACACAATAACATGGACTAGGGTGTCAAATGAACCTGTGTTTGAGAACTTGGAAGAGTTGGAAATCTCCGAGTGTGTCTTGGAGGAGCTATGTGTCGGTGAATTACCACCTGGCTCTCTACGTAATCTTAAGAAATTGATGATGGGTGATTGTTATTGGTTGGTGAAACCGCTTTTACCGTCAAAGTTGTTGCACAGATTAGAGAGTCTGGAAGAACTACGTTGTTCTCGTATGCGGGCAATGAAATATGTGTTTGGATTTGAAGGGCATGAACCACAACACGTTGTCTTGACGAGGCTGAGTATGATTTCATTGAGATCCATGAACAGCTTAATAAACATATGGGATGGTCCTGCTCCATCTGGAATCTTCCAGAGTCTCAAAATCTTTTCCATGGTTAATTGCCAGAAACTGGAATATCTCTTCACATCTGATGTAGCTCGGTGTCTTTTGCAATTGGAAGACGTTTTGGTAGAGGAATGCGAATGCTTGGACAGAATAATTGAAGCGAGTGAGGAAATAGAGAGCAACAAGATCGTTCTTCCACAGTTGAAGAACTTGGTTTTAAAACGTCTTCCAAAGCTCACAAGGCTGTCCAGTACAAGCAGCAGTACTATTGATATTGAAATCGAGTGCCCTACATTGGAACACTTACATGTCTCTGAGTGCCCCCAGTTATCAATCTCTGCTTCTGATTTCAACAGCAGCAACGAAGTCCAACTAGATGATCACCGGCATTGGAGCACACTAACGTATAGGATCGACGACGTGAGGTGCCGCTCAACCTCTCTACTTCTGATGAGTGCGAACTTGGCACGGGCTCAACAGCCACGGGCTCCGTCATTCGAGTTTAAAATGAGAGCTGCATTGTAG

Protein Analysis

1144

Amino Acids

129.66

Weight (kDa)

6.26

Isoelectric Point (pI)

50.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 21 - 193 2.9e-52 TIR domain
TIR_2 PF13676 24 - 120 6.8e-17 TIR domain
NB-ARC PF00931 209 - 373 4.5e-32 NB-ARC domain
LRR_8 PF13855 581 - 638 3.5e-07 Leucine rich repeat
LRR_14 PF23598 605 - 706 3.1e-13 Leucine-rich repeat region
LRR_RPS2 PF23247 870 - 1016 5.9e-24 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 348, 917
AasI GACNNNNNNGTC 1 cut(s) 2584
Acc36I ACCTGC 1 cut(s) 1288
AccB1I GGYRCC 1 cut(s) 3336
AccB7I CCANNNNNTGG 3 cut(s) 748, 1042, 2974
AccBSI CCGCTC 1 cut(s) 3342
AccI GTMKAC 1 cut(s) 1136
AciI CCGC 5 cut(s) 824, 2153, 2732, 2797, 3340
AclI AACGTT 1 cut(s) 2265
AclWI GGATC 5 cut(s) 834, 904, 1780, 2874, 3329
AcoI YGGCCR 3 cut(s) 483, 1584, 1612
AcsI RAATTY 6 cut(s) 512, 963, 1059, 1997, 2017, 2026
AcuI CTGAAG 4 cut(s) 67, 161, 333, 1730
AcyI GRCGYC 1 cut(s) 1353
AfaI GTAC 5 cut(s) 2109, 2114, 2453, 3163, 3175
AfiI CCNNNNNNNGG 6 cut(s) 748, 1042, 1294, 2485, 2974, 3205
AflII CTTAAG 1 cut(s) 2690
AflIII ACRYGT 4 cut(s) 724, 1453, 2842, 3217
AhdI GACNNNNNGTC 1 cut(s) 1357
AjiI CACGTC 1 cut(s) 3331
AjnI CCWGG 2 cut(s) 227, 2671
AjuI GAANNNNNNNTTGG 8 cut(s) 247, 279, 992, 1024, 1044, 1076, 1349, 1381
AleI CACNNNNGTG 1 cut(s) 2496
AloI GAACNNNNNNTCC 2 cut(s) 2315, 2347
Alw21I GWGCWC 4 cut(s) 14, 736, 1492, 3308
Alw26I GTCTC 9 cut(s) 209, 358, 1190, 1278, 1880, 1969, 2057, 2945, 3226
Alw44I GTGCAC 1 cut(s) 732
AlwI GGATC 5 cut(s) 834, 904, 1780, 2874, 3329
AlwNI CAGNNNCTG 1 cut(s) 2974
Ama87I CYCGRG 2 cut(s) 7, 2483
AoxI GGCC 6 cut(s) 3, 483, 572, 1584, 1612, 2346
ApaLI GTGCAC 1 cut(s) 732
ApoI RAATTY 6 cut(s) 512, 963, 1059, 1997, 2017, 2026
Asp700I GAANNNNTTC 1 cut(s) 2231
AspLEI GCGC 2 cut(s) 561, 818
AspS9I GGNCC 1 cut(s) 2912
AsuC2I CCSGG 1 cut(s) 100
AsuHPI GGTGA 9 cut(s) 101, 463, 730, 1003, 1639, 2672, 2720, 2737, 3284
AsuNHI GCTAGC 1 cut(s) 2183
AvaI CYCGRG 2 cut(s) 7, 2483
AvaII GGWCC 1 cut(s) 2912
BaeGI GKGCMC 4 cut(s) 736, 1219, 3200, 3233
BaeI ACNNNNGTAYC 2 cut(s) 2443, 2476
BalI TGGCCA 3 cut(s) 485, 1586, 1614
BanI GGYRCC 1 cut(s) 3336
BanII GRGCYC 3 cut(s) 14, 3385, 3400
BarI GAAGNNNNNNTAC 2 cut(s) 2521, 2553
BbsI GAAGAC 6 cut(s) 99, 713, 1245, 1419, 3030, 3128
Bbv12I GWGCWC 4 cut(s) 14, 736, 1492, 3308
BccI CCATC 6 cut(s) 637, 1529, 1604, 2698, 2903, 2929
BceAI ACGGC 1 cut(s) 546
BcgI CGANNNNNNTGC 2 cut(s) 3079, 3113
BciT130I CCWGG 2 cut(s) 229, 2673
BciVI GTATCC 2 cut(s) 124, 793
BclI TGATCA 2 cut(s) 2376, 3289
BcnI CCSGG 1 cut(s) 100
BcoDI GTCTC 9 cut(s) 209, 358, 1190, 1278, 1880, 1969, 2057, 2945, 3226
BfaI CTAG 7 cut(s) 1170, 1250, 1503, 2178, 2184, 2580, 3284
BfmI CTRYAG 2 cut(s) 1605, 2049
BfrI CTTAAG 1 cut(s) 2690
BfuAI ACCTGC 1 cut(s) 1288
BfuI GTATCC 2 cut(s) 124, 793
BglII AGATCT 1 cut(s) 1831
BmcAI AGTACT 2 cut(s) 2109, 3175
Bme1390I CCNGG 3 cut(s) 100, 229, 2673
Bme18I GGWCC 1 cut(s) 2912
BmeRI GACNNNNNGTC 1 cut(s) 1357
BmeT110I CYCGRG 2 cut(s) 7, 2483
BmgBI CACGTC 1 cut(s) 3331
BmgT120I GGNCC 1 cut(s) 2912
BmiI GGNNCC 2 cut(s) 3338, 3399
BmrFI CCNGG 3 cut(s) 100, 229, 2673
BmrI ACTGGG 2 cut(s) 1184, 3229
BmsI GCATC 4 cut(s) 177, 430, 1078, 1551
BmtI GCTAGC 1 cut(s) 2187
BmuI ACTGGG 2 cut(s) 1184, 3229
BpiI GAAGAC 6 cut(s) 99, 713, 1245, 1419, 3030, 3128
BpuEI CTTGAG 4 cut(s) 1286, 1979, 2061, 2501
BpuMI CCSGG 1 cut(s) 100
BsaAI YACGTR 1 cut(s) 2684
BsaHI GRCGYC 1 cut(s) 1353
BsaI GGTCTC 1 cut(s) 1190
BsaJI CCNNGG 8 cut(s) 53, 169, 714, 1041, 2464, 2497, 2955, 3392
BsaXI ACNNNNNCTCC 2 cut(s) 1860, 1890
Bsc4I CCNNNNNNNGG 6 cut(s) 748, 1042, 1294, 2485, 2974, 3205
Bse118I RCCGGY 1 cut(s) 3294
Bse1I ACTGG 7 cut(s) 333, 1190, 1247, 1849, 2979, 3159, 3235
Bse3DI GCAATG 3 cut(s) 439, 1856, 2808
BseBI CCWGG 2 cut(s) 229, 2673
BseDI CCNNGG 8 cut(s) 53, 169, 714, 1041, 2464, 2497, 2955, 3392
BseGI GGATG 7 cut(s) 169, 192, 289, 1401, 1540, 1566, 2914
BseLI CCNNNNNNNGG 6 cut(s) 748, 1042, 1294, 2485, 2974, 3205
BseMI GCAATG 3 cut(s) 439, 1856, 2808
BseMII CTCAG 5 cut(s) 91, 1695, 1885, 2852, 3216
BseNI ACTGG 7 cut(s) 333, 1190, 1247, 1849, 2979, 3159, 3235
BseRI GAGGAG 4 cut(s) 42, 866, 1310, 2660
BseSI GKGCMC 4 cut(s) 736, 1219, 3200, 3233
BseYI CCCAGC 2 cut(s) 832, 968
BsgI GTGCAG 2 cut(s) 255, 2265
Bsh1285I CGRYCG 1 cut(s) 2497
BshFI GGCC 6 cut(s) 5, 485, 574, 1586, 1614, 2348
BshNI GGYRCC 1 cut(s) 3336
BsiEI CGRYCG 1 cut(s) 2497
BsiHKAI GWGCWC 4 cut(s) 14, 736, 1492, 3308
BsiHKCI CYCGRG 2 cut(s) 7, 2483
BsiSI CCGG 2 cut(s) 100, 3295
BslFI GGGAC 2 cut(s) 1552, 2300
BslI CCNNNNNNNGG 6 cut(s) 748, 1042, 1294, 2485, 2974, 3205
BsmAI GTCTC 9 cut(s) 209, 358, 1190, 1278, 1880, 1969, 2057, 2945, 3226
BsmBI CGTCTC 1 cut(s) 1969
BsmFI GGGAC 2 cut(s) 1552, 2300
BsmI GAATGC 4 cut(s) 1598, 2255, 3047, 3053
BsnI GGCC 6 cut(s) 5, 485, 574, 1586, 1614, 2348
Bso31I GGTCTC 1 cut(s) 1190
BsoBI CYCGRG 2 cut(s) 7, 2483
Bsp1286I GDGCHC 9 cut(s) 14, 736, 1219, 1492, 3200, 3233, 3308, 3385, 3400
Bsp1407I TGTACA 1 cut(s) 2112
Bsp19I CCATGG 4 cut(s) 714, 1041, 2464, 2955
BspACI CCGC 5 cut(s) 824, 2153, 2732, 2797, 3340
BspANI GGCC 6 cut(s) 5, 485, 574, 1586, 1614, 2348
BspCNI CTCAG 5 cut(s) 90, 1694, 1884, 2853, 3217
BspLI GGNNCC 2 cut(s) 3338, 3399
BspMAI CTGCAG 1 cut(s) 2053
BspMI ACCTGC 1 cut(s) 1288
BspOI GCTAGC 1 cut(s) 2187
BspPI GGATC 5 cut(s) 834, 904, 1780, 2874, 3329
BspT107I GGYRCC 1 cut(s) 3336
BspTI CTTAAG 1 cut(s) 2690
BspTNI GGTCTC 1 cut(s) 1190
BsrBI CCGCTC 1 cut(s) 3342
BsrDI GCAATG 3 cut(s) 439, 1856, 2808
BsrFI RCCGGY 1 cut(s) 3294
BsrGI TGTACA 1 cut(s) 2112
BsrI ACTGG 7 cut(s) 333, 1190, 1247, 1849, 2979, 3159, 3235
BssAI RCCGGY 1 cut(s) 3294
BssECI CCNNGG 8 cut(s) 53, 169, 714, 1041, 2464, 2497, 2955, 3392
BssNI GRCGYC 1 cut(s) 1353
BssT1I CCWWGG 5 cut(s) 169, 714, 1041, 2464, 2955
Bst2UI CCWGG 2 cut(s) 229, 2673
Bst4CI ACNGT 5 cut(s) 1346, 2112, 2498, 2742, 3111
Bst6I CTCTTC 5 cut(s) 31, 39, 408, 2610, 2990
BstACI GRCGYC 1 cut(s) 1353
BstAFI CTTAAG 1 cut(s) 2690
BstAPI GCANNNNNTGC 3 cut(s) 187, 1202, 1710
BstAUI TGTACA 1 cut(s) 2112
BstBAI YACGTR 1 cut(s) 2684
BstC8I GCNNGC 4 cut(s) 1715, 2185, 2255, 2799
BstDSI CCRYGG 6 cut(s) 714, 1041, 2464, 2497, 2955, 3392
BstENI CCTNNNNNAGG 2 cut(s) 1292, 2483
BstF5I GGATG 7 cut(s) 169, 192, 289, 1401, 1540, 1566, 2914
BstHHI GCGC 2 cut(s) 561, 818
BstMAI GTCTC 9 cut(s) 209, 358, 1190, 1278, 1880, 1969, 2057, 2945, 3226
BstMCI CGRYCG 1 cut(s) 2497
BstMWI GCNNNNNNNGC 6 cut(s) 187, 1202, 1710, 1845, 3151, 3374
BstNI CCWGG 2 cut(s) 229, 2673
BstNSI RCATGY 3 cut(s) 728, 1457, 3221
BstSCI CCNGG 3 cut(s) 98, 227, 2671
BstSFI CTRYAG 2 cut(s) 1605, 2049
BstSLI GKGCMC 4 cut(s) 736, 1219, 3200, 3233
BstSNI TACGTA 1 cut(s) 2684
BstV2I GAAGAC 6 cut(s) 99, 713, 1245, 1419, 3030, 3128
BstX2I RGATCY 5 cut(s) 826, 909, 1785, 1831, 2879
BstXI CCANNNNNNTGG 1 cut(s) 1729
BstYI RGATCY 5 cut(s) 826, 909, 1785, 1831, 2879
BsuI GTATCC 2 cut(s) 124, 793
BsuRI GGCC 6 cut(s) 5, 485, 574, 1586, 1614, 2348
BtgI CCRYGG 6 cut(s) 714, 1041, 2464, 2497, 2955, 3392
BtrI CACGTC 1 cut(s) 3331
BtsCI GGATG 7 cut(s) 169, 192, 289, 1401, 1540, 1566, 2914
BtsIMutI CAGTG 4 cut(s) 326, 1351, 1842, 2425
BveI ACCTGC 1 cut(s) 1288
Cac8I GCNNGC 4 cut(s) 1715, 2185, 2255, 2799
CaiI CAGNNNCTG 1 cut(s) 2974
CfoI GCGC 2 cut(s) 561, 818
Cfr10I RCCGGY 1 cut(s) 3294
Cfr13I GGNCC 1 cut(s) 2912
CseI GACGC 2 cut(s) 1361, 1986
Csp6I GTAC 5 cut(s) 2108, 2113, 2452, 3162, 3174
CviQI GTAC 5 cut(s) 2108, 2113, 2452, 3162, 3174
DraI TTTAAA 3 cut(s) 2217, 3129, 3415
DrdI GACNNNNNNGTC 1 cut(s) 2584
DriI GACNNNNNGTC 1 cut(s) 1357
DseDI GACNNNNNNGTC 1 cut(s) 2584
EaeI YGGCCR 3 cut(s) 483, 1584, 1612
Eam1104I CTCTTC 5 cut(s) 31, 39, 408, 2610, 2990
Eam1105I GACNNNNNGTC 1 cut(s) 1357
EarI CTCTTC 5 cut(s) 31, 39, 408, 2610, 2990
Ecl136II GAGCTC 1 cut(s) 12
Eco105I TACGTA 1 cut(s) 2684
Eco130I CCWWGG 5 cut(s) 169, 714, 1041, 2464, 2955
Eco147I AGGCCT 2 cut(s) 574, 2348
Eco24I GRGCYC 3 cut(s) 14, 3385, 3400
Eco31I GGTCTC 1 cut(s) 1190
Eco32I GATATC 1 cut(s) 2085
Eco47I GGWCC 1 cut(s) 2912
Eco53kI GAGCTC 1 cut(s) 12
Eco57I CTGAAG 4 cut(s) 67, 161, 333, 1730
Eco88I CYCGRG 2 cut(s) 7, 2483
EcoICRI GAGCTC 1 cut(s) 12
EcoNI CCTNNNNNAGG 2 cut(s) 1292, 2483
EcoRI GAATTC 1 cut(s) 963
EcoRII CCWGG 2 cut(s) 227, 2671
EcoRV GATATC 1 cut(s) 2085
EcoT14I CCWWGG 5 cut(s) 169, 714, 1041, 2464, 2955
EcoT22I ATGCAT 1 cut(s) 1566
EcoT38I GRGCYC 3 cut(s) 14, 3385, 3400
ErhI CCWWGG 5 cut(s) 169, 714, 1041, 2464, 2955
Esp3I CGTCTC 1 cut(s) 1969
FalI AAGNNNNNCTT 4 cut(s) 804, 836, 1349, 1381
FaqI GGGAC 2 cut(s) 1552, 2300
FauI CCCGC 2 cut(s) 2146, 2790
FauNDI CATATG 1 cut(s) 2903
FbaI TGATCA 2 cut(s) 2376, 3289
FblI GTMKAC 1 cut(s) 1136
FokI GGATG 7 cut(s) 176, 199, 296, 1388, 1547, 1573, 2921
FriOI GRGCYC 3 cut(s) 14, 3385, 3400
FspBI CTAG 7 cut(s) 1170, 1250, 1503, 2178, 2184, 2580, 3284
GlaI GCGC 2 cut(s) 560, 817
GsaI CCCAGC 2 cut(s) 836, 972
HaeIII GGCC 6 cut(s) 5, 485, 574, 1586, 1614, 2348
HapII CCGG 2 cut(s) 100, 3295
HgaI GACGC 2 cut(s) 1361, 1986
HhaI GCGC 2 cut(s) 561, 818
Hin1I GRCGYC 1 cut(s) 1353
Hin6I GCGC 2 cut(s) 559, 816
HinP1I GCGC 2 cut(s) 559, 816
HincII GTYRAC 2 cut(s) 1351, 1459
HindII GTYRAC 2 cut(s) 1351, 1459
HindIII AAGCTT 5 cut(s) 1515, 1715, 1893, 1955, 2228
HpaII CCGG 2 cut(s) 100, 3295
HphI GGTGA 9 cut(s) 101, 463, 730, 1003, 1639, 2672, 2720, 2737, 3284
Hpy166II GTNNAC 6 cut(s) 734, 866, 1137, 1351, 1459, 1669
Hpy188III TCNNGA 8 cut(s) 1577, 1699, 2367, 2483, 2771, 2851, 2925, 2935
Hpy8I GTNNAC 6 cut(s) 734, 866, 1137, 1351, 1459, 1669
Hpy99I CGWCG 2 cut(s) 3329, 3332
HpyCH4III ACNGT 5 cut(s) 1346, 2112, 2498, 2742, 3111
HpyCH4IV ACGT 8 cut(s) 2265, 2683, 2782, 2844, 3027, 3133, 3314, 3330
HpyF10VI GCNNNNNNNGC 6 cut(s) 187, 1202, 1710, 1845, 3151, 3374
HpySE526I ACGT 8 cut(s) 2265, 2683, 2782, 2844, 3027, 3133, 3314, 3330
Hsp92I GRCGYC 1 cut(s) 1353
HspAI GCGC 2 cut(s) 559, 816
Ksp22I TGATCA 2 cut(s) 2376, 3289
LmnI GCTCC 7 cut(s) 17, 55, 2074, 2647, 2923, 3303, 3403
LweI GCATC 4 cut(s) 177, 430, 1078, 1551
MaeI CTAG 7 cut(s) 1170, 1250, 1503, 2178, 2184, 2580, 3284
MaeII ACGT 8 cut(s) 2265, 2683, 2782, 2844, 3027, 3133, 3314, 3330
MaeIII GTNAC 3 cut(s) 1498, 1982, 2425
MbiI CCGCTC 1 cut(s) 3342
MfeI CAATTG 1 cut(s) 3017
MflI RGATCY 5 cut(s) 826, 909, 1785, 1831, 2879
MhlI GDGCHC 9 cut(s) 14, 736, 1219, 1492, 3200, 3233, 3308, 3385, 3400
MlsI TGGCCA 3 cut(s) 485, 1586, 1614
MluNI TGGCCA 3 cut(s) 485, 1586, 1614
MlyI GAGTC 6 cut(s) 863, 1491, 1881, 2508, 2776, 2947
MmeI TCCRAC 4 cut(s) 562, 2019, 2601, 3304
Mox20I TGGCCA 3 cut(s) 485, 1586, 1614
Mph1103I ATGCAT 1 cut(s) 1566
MroXI GAANNNNTTC 1 cut(s) 2231
MscI TGGCCA 3 cut(s) 485, 1586, 1614
MslI CAYNNNNRTG 5 cut(s) 729, 1905, 1934, 2496, 3216
Msp20I TGGCCA 3 cut(s) 485, 1586, 1614
MspCI CTTAAG 1 cut(s) 2690
MspI CCGG 2 cut(s) 100, 3295
MspR9I CCNGG 3 cut(s) 100, 229, 2673
MssI GTTTAAAC 1 cut(s) 2217
MunI CAATTG 1 cut(s) 3017
Mva1269I GAATGC 4 cut(s) 1598, 2255, 3047, 3053
MvaI CCWGG 2 cut(s) 229, 2673
MwoI GCNNNNNNNGC 6 cut(s) 187, 1202, 1710, 1845, 3151, 3374
NciI CCSGG 1 cut(s) 100
NcoI CCATGG 4 cut(s) 714, 1041, 2464, 2955
NdeI CATATG 1 cut(s) 2903
NheI GCTAGC 1 cut(s) 2183
NlaIV GGNNCC 2 cut(s) 3338, 3399
NmuCI GTSAC 1 cut(s) 2425
NsiI ATGCAT 1 cut(s) 1566
NspI RCATGY 3 cut(s) 728, 1457, 3221
OliI CACNNNNGTG 1 cut(s) 2496
PaeR7I CTCGAG 2 cut(s) 7, 2483
PceI AGGCCT 2 cut(s) 574, 2348
PciI ACATGT 3 cut(s) 724, 1453, 3217
PcsI WCGNNNNNNNCGW 2 cut(s) 2490, 2788
PctI GAATGC 4 cut(s) 1598, 2255, 3047, 3053
PdmI GAANNNNTTC 1 cut(s) 2231
PfeI GAWTC 6 cut(s) 224, 298, 614, 1963, 2534, 2928
PflMI CCANNNNNTGG 3 cut(s) 748, 1042, 2974
PleI GAGTC 6 cut(s) 862, 1490, 1881, 2508, 2775, 2946
PmeI GTTTAAAC 1 cut(s) 2217
PpsI GAGTC 6 cut(s) 862, 1490, 1881, 2508, 2775, 2946
Ppu21I YACGTR 1 cut(s) 2684
PscI ACATGT 3 cut(s) 724, 1453, 3217
PsiI TTATAA 2 cut(s) 348, 917
Psp124BI GAGCTC 1 cut(s) 14
Psp1406I AACGTT 1 cut(s) 2265
Psp6I CCWGG 2 cut(s) 227, 2671
PspFI CCCAGC 2 cut(s) 832, 968
PspGI CCWGG 2 cut(s) 227, 2671
PspN4I GGNNCC 2 cut(s) 3338, 3399
PspPI GGNCC 1 cut(s) 2912
PspXI VCTCGAGB 1 cut(s) 7
PstI CTGCAG 1 cut(s) 2053
PstNI CAGNNNCTG 1 cut(s) 2974
PsuI RGATCY 5 cut(s) 826, 909, 1785, 1831, 2879
RsaI GTAC 5 cut(s) 2109, 2114, 2453, 3163, 3175
RsaNI GTAC 5 cut(s) 2108, 2113, 2452, 3162, 3174
RseI CAYNNNNRTG 5 cut(s) 729, 1905, 1934, 2496, 3216
SacI GAGCTC 1 cut(s) 14
Sau96I GGNCC 1 cut(s) 2912
ScaI AGTACT 2 cut(s) 2109, 3175
SchI GAGTC 6 cut(s) 863, 1491, 1881, 2508, 2776, 2947
ScrFI CCNGG 3 cut(s) 100, 229, 2673
SduI GDGCHC 9 cut(s) 14, 736, 1219, 1492, 3200, 3233, 3308, 3385, 3400
SfaNI GCATC 4 cut(s) 177, 430, 1078, 1551
SfcI CTRYAG 2 cut(s) 1605, 2049
Sfr274I CTCGAG 2 cut(s) 7, 2483
SinI GGWCC 1 cut(s) 2912
SlaI CTCGAG 2 cut(s) 7, 2483
SmiMI CAYNNNNRTG 5 cut(s) 729, 1905, 1934, 2496, 3216
SmlI CTYRAG 7 cut(s) 7, 1265, 1958, 2076, 2483, 2516, 2690
SmoI CTYRAG 7 cut(s) 7, 1265, 1958, 2076, 2483, 2516, 2690
SnaBI TACGTA 1 cut(s) 2684
SseBI AGGCCT 2 cut(s) 574, 2348
SsiI CCGC 5 cut(s) 824, 2153, 2732, 2797, 3340
SspI AATATT 1 cut(s) 808
SspMI CTAG 7 cut(s) 1170, 1250, 1503, 2178, 2184, 2580, 3284
SstI GAGCTC 1 cut(s) 14
StuI AGGCCT 2 cut(s) 574, 2348
StyD4I CCNGG 3 cut(s) 98, 227, 2671
StyI CCWWGG 5 cut(s) 169, 714, 1041, 2464, 2955
TaaI ACNGT 5 cut(s) 1346, 2112, 2498, 2742, 3111
TaiI ACGT 8 cut(s) 2268, 2686, 2785, 2847, 3030, 3136, 3317, 3333
TatI WGTACW 4 cut(s) 2107, 2112, 3161, 3173
TauI GCSGC 1 cut(s) 3342
TfiI GAWTC 6 cut(s) 224, 298, 614, 1963, 2534, 2928
TscAI CASTG 4 cut(s) 333, 1351, 1849, 2425
TseFI GTSAC 1 cut(s) 2425
Tsp45I GTSAC 1 cut(s) 2425
TspGWI ACGGA 3 cut(s) 902, 1394, 3390
TspRI CASTG 4 cut(s) 333, 1351, 1849, 2425
Van91I CCANNNNNTGG 3 cut(s) 748, 1042, 2974
Vha464I CTTAAG 1 cut(s) 2690
VneI GTGCAC 1 cut(s) 732
VpaK11BI GGWCC 1 cut(s) 2912
XagI CCTNNNNNAGG 2 cut(s) 1292, 2483
XapI RAATTY 6 cut(s) 512, 963, 1059, 1997, 2017, 2026
XceI RCATGY 3 cut(s) 728, 1457, 3221
XcmI CCANNNNNNNNNTGG 1 cut(s) 325
XhoI CTCGAG 2 cut(s) 7, 2483
XmiI GTMKAC 1 cut(s) 1136
XmnI GAANNNNTTC 1 cut(s) 2231
XspI CTAG 7 cut(s) 1170, 1250, 1503, 2178, 2184, 2580, 3284
ZrmI AGTACT 2 cut(s) 2109, 3175
Zsp2I ATGCAT 1 cut(s) 1566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.