FvH4_3g35890

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000085
Physical Location & Seq
Forward (+)
30785017 .. 30793116
8100 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g35890.t4

Sequence Viewer

Length: 2892 bp
ATGGACAAGGTAATGGGGGCACTTAAAGATGATGAGATCACTGCCATTGGGGTATATGGAATGGGTGGCGTTGGCAAGACAAGTATGGTGAAACATGTCGGTGCACATGCCCGAAAACTTGGTCTTTTTCATCATGTGATTATGGCTGTTGTGTCCCAAACACCTAATTTGAGAGATATTCAAGGCACGCTGGCTGATCTGCTGGGGTTTAAATTGTTGGAGGAGACCGTAGACGGTAGAGCGGCTAGATTGATGGAGAAGATAATGAAAGGAAATAGGATCCTTATAATTTTGGACGACATGTGGGAGAGAATAGACTTGCCCAGAATTGGAATACCGAGCCAGCATGAGCTTCAAAAGTGCAGTTCCAAATTCGTATTCACCACAAGGAGATTGAATGTTTGTCATTCGATGGAGAGCCAAGCAACGATTCCTCTCTTAGGTTTATCAAATGATGATGCTTGGAACCTGTTTGTGAAGAAAACAATAAAGTCTTTTCATGAATCAACAATCTTTTATAATGTAGCGAGGACTGTAGCTAAAGAATGTGCTGGTCTCCCAGTAGCATTGGTAGCAGTTGCAAGGGCACTTGGAGATAAGGACTTGGATGATTGGACTGAAGCAGCTAGACGATTGAAGGCTGCTCAACCTGCCAACTATGAAGATAAGAGAGTTGTGTTCAGATGTATAAAGTTGAGCTATGATTACTTGACACCTGATGATGACAACATCTCCAAGAAATTATTCTTGCTTTGCTGTCTGTTTCCTGAAGATTCTGATATTAAGGTCGAAGACTTGTTGGTGTATGCACTTGGGAAAGGACTATTTCAAGATGCTGACACAATCAATGCAGCCAGAGCCAAAGCCCATTTGGTGGTCAATTACCTGAAAGATTCTAGCTTACTTTTGGAAAGTGAACACTACGGATTTGTGAAGATGCATGACGTAATTCGAGATGTGGCCGTATCAATTTCATTATCGGAAGATGGATCACGGTTTTTGGTTAAAGCTGGTTGTGAATTAAAGTATTGGCCAAGGATCACTCATCAAGACTACTGCGCAATCTCCCTAATGAGGAACGAAATTCACGAGCTTCCCGAAAGGTTGGTGTGTTCAAAACTCCAGATTTTATTACTACAGAGCAATAGCATAAATGAGATTCCAATCCTTTTCTTTCAAAGTTCCAAAGAATTGAGGGTCTTAGATCTTAGCGAGACTAGAATTTTGTGTCTGCCGATATCATTAGGTCTCCTAACCAACCTCCAAGCTCTCTATTTAGATGAATGCAAGAGATTAGTTGATATATTCATACTTGGAAATTTGAAGAACCTTCAGATTCTTAGTATGAAGAACTTTGGAGGAACCACTCTTCAAAAAGAAATGCCGCAAGAAATAGGATACTTGACTAATCTTAAGATGTTGGACGTCACAGATGCATGTTTTGAAACAGTTCCCCCTAAATTGATATCCAACTTGAATGGATTAGAAGAATTGTACATGAAATGTGACTTTTGCGACTGGGGCTGTAGAACAGAAGAGGGTGGTTTGTTTTCTTTTGTTTTTCCCAATAATGCTAGCTTTGATGAGATAACAGGGTTATCACGTTTAAACAGTTTGAAAGTTCAGATATATGATGTAAAATGCTTGCCTCCACTGGTTGGGTTCAGTCCCAATTGGGTCAACTTCGATATCTGTATCAGCACAAAAGAATTTATGAAACAGTCCTATTACGGACGCTTTTTCGAGAGGAAACTAACTGTCAAGATGGATGCACAACGTCATTTCTCTCGGTATTTGACTCTAAACACAAGCATGAGAAATTTACCAGACTGGTTTATCAATGTAGTGACAGAGAAAGCAGAGATACTAGAGTACATAGGGTACAGGGGATTAGAGAACATTCTTGTGGAATATCACCATGGGAGATTACATGAGCTAAAGTACCTTGGTGTCATTGAGTGCGAGGTGGTTGGAGAGTTGATGAACTCCCAAACAACATGGGTTCCGAATAGACCTGTGTTTGAGAGCTTGGAAGAGTTGTATCTAGAAGGAGTCGATGGCCTGAAAGAGTTATGTGTTGGTGAGTTACCAGCTGGGTCTCTGGGAAATCTTAAGTTGTTAAGTGTGAGAAATTGCCCGCATCTTTTAAATGCACTCTTACCATCACATCTGTTGCCCAGCCTACAAAATCTGGAGATACTACATTGTGAGTACACAAAGGATGCGAGAGGAACCGCATATGTGTTTGGATTTGAATACAGGTTGACAGAAAAAATCGTCTTACCGAAATTGAGACAGATAACATTATGTTATCTTGTGAACCTACAAAGCATATCCTATGGTTATACTCCTAGTGGAGTCTTCCGTAATCTCAACAGTTTGGCAATTCATGGGAGCCAGAAATTGAAATATCTGTTGACGTACGAAGTAGCTATATCTCTTTTCCAATTGGAACTCCTCTGGGTGGAGGAGTGTTCCGACATGGAAACAGTTATTCAATTAGGCTCGAATACAAATGGAAGCAGAATAGTTTTTCCAAAATTGAAGGAAATGTATTTGAATAGTCTTCCACGGCTCACATGGATCTACAATTGGGATACCGTTCAGTGCCCTTCATTGGAACACTTGTATGTGTGCAAGTGCCCCAGACTTGTAGACCATGCTGCTGCTTTCCAAAGCAGAAACTACGTCAAAACAAATGATGAAGGGCATTTTGCGATTATATTTGAGAGATTGTTCCACCGCCAATTCAACCCAGAAGAGATTCCTCAGTTCTTCTCCAAAAAAGAAAGTGATTTCTCAACAGGCTCTTCATTGAAGGCTGATCCAGGCTCTTCATTGAAGGCGGATCCAATGCAAGGCTCACCTAGGCTTTGGGTTAAGCCCAGGTGA

Protein Analysis

964

Amino Acids

109.57

Weight (kDa)

6.28

Isoelectric Point (pI)

37.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 2 - 151 8.6e-32 TIR domain
TIR_2 PF13676 6 - 67 2.4e-08 TIR domain
NB-ARC PF00931 172 - 336 3.2e-27 NB-ARC domain
LRR_8 PF13855 545 - 601 1e-06 Leucine rich repeat
LRR_14 PF23598 546 - 671 1.1e-09 Leucine-rich repeat region
LRR_RPS2 PF23247 849 - 997 4.6e-19 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 287, 519
AatII GACGTC 1 cut(s) 1428
Acc16I TGCGCA 1 cut(s) 1060
Acc36I ACCTGC 1 cut(s) 658
AccB7I CCANNNNNTGG 2 cut(s) 874, 1658
AccBSI CCGCTC 1 cut(s) 242
AccI GTMKAC 2 cut(s) 231, 2655
AciI CCGC 6 cut(s) 242, 1385, 2138, 2235, 2743, 2846
AclWI GGATC 8 cut(s) 274, 287, 997, 1046, 2591, 2819, 2843, 2856
AcoI YGGCCR 2 cut(s) 960, 1031
AcsI RAATTY 6 cut(s) 371, 1083, 1221, 1318, 1709, 1819
AcuI CTGAAG 3 cut(s) 639, 789, 1316
AcyI GRCGYC 1 cut(s) 1425
AfaI GTAC 6 cut(s) 1496, 1874, 1883, 1943, 2213, 2423
AfiI CCNNNNNNNGG 8 cut(s) 329, 440, 874, 1074, 1658, 2464, 2617, 2858
AflII CTTAAG 2 cut(s) 1412, 2111
AflIII ACRYGT 2 cut(s) 94, 300
AjnI CCWGG 2 cut(s) 2827, 2885
AjuI GAANNNNNNNTTGG 6 cut(s) 362, 394, 414, 446, 728, 760
Alw21I GWGCWC 1 cut(s) 106
Alw26I GTCTC 6 cut(s) 218, 560, 1208, 1253, 2103, 2287
Alw44I GTGCAC 1 cut(s) 102
AlwI GGATC 8 cut(s) 274, 287, 997, 1046, 2591, 2819, 2843, 2856
AoxI GGCC 3 cut(s) 960, 1031, 2059
ApaLI GTGCAC 1 cut(s) 102
ApeKI GCWGC 5 cut(s) 623, 641, 851, 2663, 2666
ApoI RAATTY 6 cut(s) 371, 1083, 1221, 1318, 1709, 1819
ArsI GACNNNNNNTTYG 4 cut(s) 106, 138, 1207, 1239
Asp700I GAANNNNTTC 3 cut(s) 743, 1449, 2763
AspA2I CCTAGG 1 cut(s) 2867
AspLEI GCGC 1 cut(s) 1061
AsuHPI GGTGA 5 cut(s) 100, 373, 1907, 2093, 2856
AsuNHI GCTAGC 1 cut(s) 1574
AvrII CCTAGG 1 cut(s) 2867
BaeGI GKGCMC 5 cut(s) 22, 106, 589, 2612, 2645
BaeI ACNNNNGTAYC 2 cut(s) 1856, 1889
BalI TGGCCA 1 cut(s) 1033
BamHI GGATCC 2 cut(s) 279, 2848
BauI CACGAG 1 cut(s) 1088
BbsI GAAGAC 3 cut(s) 798, 2353, 2558
Bbv12I GWGCWC 1 cut(s) 106
BbvI GCAGC 5 cut(s) 628, 635, 863, 2650, 2653
BccI CCATC 6 cut(s) 247, 406, 980, 1759, 2051, 2170
BceAI ACGGC 2 cut(s) 947, 2588
BciT130I CCWGG 2 cut(s) 2829, 2887
BciVI GTATCC 2 cut(s) 1391, 2590
BcoDI GTCTC 6 cut(s) 218, 560, 1208, 1253, 2103, 2287
BfaI CTAG 9 cut(s) 246, 627, 897, 1218, 1575, 1868, 2045, 2352, 2868
BfmI CTRYAG 3 cut(s) 534, 1136, 1525
BfrI CTTAAG 2 cut(s) 1412, 2111
BfuAI ACCTGC 1 cut(s) 658
BfuI GTATCC 2 cut(s) 1391, 2590
BglII AGATCT 1 cut(s) 1204
BisI GCNGC 7 cut(s) 243, 624, 642, 852, 1385, 2664, 2667
BlnI CCTAGG 1 cut(s) 2867
BlsI GCNGC 7 cut(s) 244, 625, 643, 853, 1386, 2665, 2668
Bme1390I CCNGG 2 cut(s) 2829, 2887
BmiI GGNNCC 7 cut(s) 281, 467, 1363, 2004, 2233, 2396, 2850
BmrFI CCNGG 2 cut(s) 2829, 2887
BmrI ACTGGG 2 cut(s) 554, 1528
BmsI GCATC 7 cut(s) 448, 823, 927, 1423, 1759, 2149, 2212
BmtI GCTAGC 1 cut(s) 1578
BmuI ACTGGG 2 cut(s) 554, 1528
BpiI GAAGAC 3 cut(s) 798, 2353, 2558
BpmI CTGGAG 2 cut(s) 1106, 2213
BsaBI GATNNNNATC 1 cut(s) 1163
BsaHI GRCGYC 1 cut(s) 1425
BsaI GGTCTC 4 cut(s) 218, 560, 1253, 2103
BsaJI CCNNGG 6 cut(s) 1034, 1918, 1945, 2570, 2867, 2885
BsaXI ACNNNNNCTCC 2 cut(s) 716, 746
Bsc4I CCNNNNNNNGG 8 cut(s) 329, 440, 874, 1074, 1658, 2464, 2617, 2858
Bse1I ACTGG 4 cut(s) 560, 1523, 1659, 1835
Bse8I GATNNNNATC 1 cut(s) 1163
BseBI CCWGG 2 cut(s) 2829, 2887
BseDI CCNNGG 6 cut(s) 1034, 1918, 1945, 2570, 2867, 2885
BseGI GGATG 3 cut(s) 613, 1774, 2227
BseJI GATNNNNATC 1 cut(s) 1163
BseLI CCNNNNNNNGG 8 cut(s) 329, 440, 874, 1074, 1658, 2464, 2617, 2858
BseMII CTCAG 1 cut(s) 2783
BseNI ACTGG 4 cut(s) 560, 1523, 1659, 1835
BseRI GAGGAG 3 cut(s) 236, 2447, 2483
BseSI GKGCMC 5 cut(s) 22, 106, 589, 2612, 2645
BseXI GCAGC 5 cut(s) 628, 635, 863, 2650, 2653
BseYI CCCAGC 3 cut(s) 202, 2093, 2177
BsgI GTGCAG 1 cut(s) 382
BshFI GGCC 3 cut(s) 962, 1033, 2061
BsiHKAI GWGCWC 1 cut(s) 106
BsiWI CGTACG 1 cut(s) 2421
BslFI GGGAC 2 cut(s) 139, 1653
BslI CCNNNNNNNGG 8 cut(s) 329, 440, 874, 1074, 1658, 2464, 2617, 2858
BsmAI GTCTC 6 cut(s) 218, 560, 1208, 1253, 2103, 2287
BsmFI GGGAC 2 cut(s) 139, 1653
BsmI GAATGC 1 cut(s) 1289
BsnI GGCC 3 cut(s) 962, 1033, 2061
Bso31I GGTCTC 4 cut(s) 218, 560, 1253, 2103
Bsp1286I GDGCHC 5 cut(s) 22, 106, 589, 2612, 2645
Bsp1407I TGTACA 1 cut(s) 1494
Bsp143I GATC 9 cut(s) 36, 196, 279, 989, 1038, 1204, 2583, 2824, 2848
Bsp19I CCATGG 1 cut(s) 1918
BspACI CCGC 6 cut(s) 242, 1385, 2138, 2235, 2743, 2846
BspANI GGCC 3 cut(s) 962, 1033, 2061
BspCNI CTCAG 1 cut(s) 2782
BspHI TCATGA 1 cut(s) 499
BspLI GGNNCC 7 cut(s) 281, 467, 1363, 2004, 2233, 2396, 2850
BspMI ACCTGC 1 cut(s) 658
BspOI GCTAGC 1 cut(s) 1578
BspPI GGATC 8 cut(s) 274, 287, 997, 1046, 2591, 2819, 2843, 2856
BspQI GCTCTTC 2 cut(s) 2815, 2839
BspTI CTTAAG 2 cut(s) 1412, 2111
BspTNI GGTCTC 4 cut(s) 218, 560, 1253, 2103
BsrBI CCGCTC 1 cut(s) 242
BsrGI TGTACA 1 cut(s) 1494
BsrI ACTGG 4 cut(s) 560, 1523, 1659, 1835
BssECI CCNNGG 6 cut(s) 1034, 1918, 1945, 2570, 2867, 2885
BssMI GATC 9 cut(s) 36, 196, 279, 989, 1038, 1204, 2583, 2824, 2848
BssNI GRCGYC 1 cut(s) 1425
BssSI CACGAG 1 cut(s) 1088
BssT1I CCWWGG 4 cut(s) 1034, 1918, 1945, 2867
Bst2BI CACGAG 1 cut(s) 1088
Bst2UI CCWGG 2 cut(s) 2829, 2887
Bst6I CTCTTC 6 cut(s) 1374, 1530, 2028, 2754, 2815, 2839
BstACI GRCGYC 1 cut(s) 1425
BstAFI CTTAAG 2 cut(s) 1412, 2111
BstAUI TGTACA 1 cut(s) 1494
BstC8I GCNNGC 6 cut(s) 188, 192, 344, 1576, 1646, 2138
BstDEI CTNAG 5 cut(s) 439, 1201, 1208, 1340, 2769
BstDSI CCRYGG 2 cut(s) 1918, 2570
BstENI CCTNNNNNAGG 1 cut(s) 438
BstF5I GGATG 3 cut(s) 613, 1774, 2227
BstHHI GCGC 1 cut(s) 1061
BstKTI GATC 9 cut(s) 39, 199, 282, 992, 1041, 1207, 2586, 2827, 2851
BstMAI GTCTC 6 cut(s) 218, 560, 1208, 1253, 2103, 2287
BstMBI GATC 9 cut(s) 36, 196, 279, 989, 1038, 1204, 2583, 2824, 2848
BstMWI GCNNNNNNNGC 4 cut(s) 572, 650, 857, 1521
BstNI CCWGG 2 cut(s) 2829, 2887
BstNSI RCATGY 4 cut(s) 98, 110, 304, 1440
BstSCI CCNGG 2 cut(s) 2827, 2885
BstSFI CTRYAG 3 cut(s) 534, 1136, 1525
BstSLI GKGCMC 5 cut(s) 22, 106, 589, 2612, 2645
BstV1I GCAGC 5 cut(s) 628, 635, 863, 2650, 2653
BstV2I GAAGAC 3 cut(s) 798, 2353, 2558
BstX2I RGATCY 4 cut(s) 279, 1204, 2583, 2848
BstYI RGATCY 4 cut(s) 279, 1204, 2583, 2848
BsuI GTATCC 2 cut(s) 1391, 2590
BsuRI GGCC 3 cut(s) 962, 1033, 2061
BtgI CCRYGG 2 cut(s) 1918, 2570
BtsCI GGATG 3 cut(s) 613, 1774, 2227
BtsI GCAGTG 1 cut(s) 39
BtsIMutI CAGTG 3 cut(s) 39, 1652, 2612
BveI ACCTGC 1 cut(s) 658
Cac8I GCNNGC 6 cut(s) 188, 192, 344, 1576, 1646, 2138
CciI TCATGA 1 cut(s) 499
CfoI GCGC 1 cut(s) 1061
CseI GACGC 1 cut(s) 1743
Csp6I GTAC 6 cut(s) 1495, 1873, 1882, 1942, 2212, 2422
CviQI GTAC 6 cut(s) 1495, 1873, 1882, 1942, 2212, 2422
DdeI CTNAG 5 cut(s) 439, 1201, 1208, 1340, 2769
DpnI GATC 9 cut(s) 38, 198, 281, 991, 1040, 1206, 2585, 2826, 2850
DpnII GATC 9 cut(s) 36, 196, 279, 989, 1038, 1204, 2583, 2824, 2848
DraI TTTAAA 3 cut(s) 211, 1608, 2148
EaeI YGGCCR 2 cut(s) 960, 1031
Eam1104I CTCTTC 6 cut(s) 1374, 1530, 2028, 2754, 2815, 2839
EarI CTCTTC 6 cut(s) 1374, 1530, 2028, 2754, 2815, 2839
EciI GGCGGA 1 cut(s) 2861
Eco130I CCWWGG 4 cut(s) 1034, 1918, 1945, 2867
Eco31I GGTCTC 4 cut(s) 218, 560, 1253, 2103
Eco32I GATATC 3 cut(s) 1239, 1467, 1690
Eco57I CTGAAG 3 cut(s) 639, 789, 1316
EcoNI CCTNNNNNAGG 1 cut(s) 438
EcoRII CCWGG 2 cut(s) 2827, 2885
EcoRV GATATC 3 cut(s) 1239, 1467, 1690
EcoT14I CCWWGG 4 cut(s) 1034, 1918, 1945, 2867
EcoT22I ATGCAT 2 cut(s) 942, 1438
ErhI CCWWGG 4 cut(s) 1034, 1918, 1945, 2867
FaqI GGGAC 2 cut(s) 139, 1653
FauI CCCGC 1 cut(s) 2145
FauNDI CATATG 1 cut(s) 2239
FblI GTMKAC 2 cut(s) 231, 2655
Fnu4HI GCNGC 7 cut(s) 243, 624, 642, 852, 1385, 2664, 2667
FokI GGATG 3 cut(s) 620, 1781, 2234
Fsp4HI GCNGC 7 cut(s) 243, 624, 642, 852, 1385, 2664, 2667
FspBI CTAG 9 cut(s) 246, 627, 897, 1218, 1575, 1868, 2045, 2352, 2868
FspI TGCGCA 1 cut(s) 1060
GlaI GCGC 1 cut(s) 1060
GluI GCNGC 7 cut(s) 243, 624, 642, 852, 1385, 2664, 2667
GsaI CCCAGC 3 cut(s) 206, 2097, 2181
GsuI CTGGAG 2 cut(s) 1106, 2213
HaeIII GGCC 3 cut(s) 962, 1033, 2061
HgaI GACGC 1 cut(s) 1743
HhaI GCGC 1 cut(s) 1061
Hin1I GRCGYC 1 cut(s) 1425
Hin6I GCGC 1 cut(s) 1059
HinP1I GCGC 1 cut(s) 1059
HincII GTYRAC 3 cut(s) 1681, 2265, 2418
HindII GTYRAC 3 cut(s) 1681, 2265, 2418
HphI GGTGA 5 cut(s) 100, 373, 1907, 2093, 2856
Hpy166II GTNNAC 9 cut(s) 104, 232, 917, 1681, 2214, 2265, 2320, 2418, 2656
Hpy188I TCNGA 7 cut(s) 683, 778, 982, 1335, 1626, 2007, 2479
Hpy8I GTNNAC 9 cut(s) 104, 232, 917, 1681, 2214, 2265, 2320, 2418, 2656
HpyAV CCTTC 8 cut(s) 631, 1340, 2042, 2539, 2622, 2699, 2812, 2836
HpyCH4IV ACGT 6 cut(s) 945, 1425, 1603, 1777, 2420, 2688
HpyF10VI GCNNNNNNNGC 4 cut(s) 572, 650, 857, 1521
HpyF3I CTNAG 5 cut(s) 439, 1201, 1208, 1340, 2769
HpySE526I ACGT 6 cut(s) 945, 1425, 1603, 1777, 2420, 2688
Hsp92I GRCGYC 1 cut(s) 1425
HspAI GCGC 1 cut(s) 1059
Kzo9I GATC 9 cut(s) 36, 196, 279, 989, 1038, 1204, 2583, 2824, 2848
LguI GCTCTTC 2 cut(s) 2815, 2839
LmnI GCTCC 1 cut(s) 2394
Lsp1109I GCAGC 5 cut(s) 628, 635, 863, 2650, 2653
LweI GCATC 7 cut(s) 448, 823, 927, 1423, 1759, 2149, 2212
MaeI CTAG 9 cut(s) 246, 627, 897, 1218, 1575, 1868, 2045, 2352, 2868
MaeII ACGT 6 cut(s) 945, 1425, 1603, 1777, 2420, 2688
MaeIII GTNAC 4 cut(s) 1426, 1505, 1846, 2085
MalI GATC 9 cut(s) 38, 198, 281, 991, 1040, 1206, 2585, 2826, 2850
MbiI CCGCTC 1 cut(s) 242
MboI GATC 9 cut(s) 36, 196, 279, 989, 1038, 1204, 2583, 2824, 2848
MfeI CAATTG 3 cut(s) 1672, 2447, 2590
MflI RGATCY 4 cut(s) 279, 1204, 2583, 2848
MhlI GDGCHC 5 cut(s) 22, 106, 589, 2612, 2645
MlsI TGGCCA 1 cut(s) 1033
MluNI TGGCCA 1 cut(s) 1033
MlyI GAGTC 3 cut(s) 1792, 2061, 2367
MmeI TCCRAC 5 cut(s) 198, 1401, 1494, 1951, 2502
Mox20I TGGCCA 1 cut(s) 1033
Mph1103I ATGCAT 2 cut(s) 942, 1438
MroXI GAANNNNTTC 3 cut(s) 743, 1449, 2763
MscI TGGCCA 1 cut(s) 1033
MslI CAYNNNNRTG 4 cut(s) 99, 1811, 1904, 2628
Msp20I TGGCCA 1 cut(s) 1033
MspA1I CMGCKG 1 cut(s) 2093
MspCI CTTAAG 2 cut(s) 1412, 2111
MspR9I CCNGG 2 cut(s) 2829, 2887
MssI GTTTAAAC 1 cut(s) 1608
MunI CAATTG 3 cut(s) 1672, 2447, 2590
Mva1269I GAATGC 1 cut(s) 1289
MvaI CCWGG 2 cut(s) 2829, 2887
MwoI GCNNNNNNNGC 4 cut(s) 572, 650, 857, 1521
NcoI CCATGG 1 cut(s) 1918
NdeI CATATG 1 cut(s) 2239
NdeII GATC 9 cut(s) 36, 196, 279, 989, 1038, 1204, 2583, 2824, 2848
NheI GCTAGC 1 cut(s) 1574
NlaIV GGNNCC 7 cut(s) 281, 467, 1363, 2004, 2233, 2396, 2850
NmuCI GTSAC 3 cut(s) 1426, 1505, 1846
NsbI TGCGCA 1 cut(s) 1060
NsiI ATGCAT 2 cut(s) 942, 1438
NspI RCATGY 4 cut(s) 98, 110, 304, 1440
PagI TCATGA 1 cut(s) 499
PciI ACATGT 2 cut(s) 94, 300
PciSI GCTCTTC 2 cut(s) 2815, 2839
PcsI WCGNNNNNNNCGW 2 cut(s) 1086, 1095
PctI GAATGC 1 cut(s) 1289
PdmI GAANNNNTTC 3 cut(s) 743, 1449, 2763
PfeI GAWTC 7 cut(s) 430, 503, 773, 893, 1159, 1336, 2764
Pfl23II CGTACG 1 cut(s) 2421
PflMI CCANNNNNTGG 2 cut(s) 874, 1658
PkrI GCNGC 7 cut(s) 244, 625, 643, 853, 1386, 2665, 2668
PleI GAGTC 3 cut(s) 1792, 2060, 2366
PmeI GTTTAAAC 1 cut(s) 1608
PpsI GAGTC 3 cut(s) 1792, 2060, 2366
PscI ACATGT 2 cut(s) 94, 300
PsiI TTATAA 2 cut(s) 287, 519
Psp6I CCWGG 2 cut(s) 2827, 2885
PspFI CCCAGC 3 cut(s) 202, 2093, 2177
PspGI CCWGG 2 cut(s) 2827, 2885
PspLI CGTACG 1 cut(s) 2421
PspN4I GGNNCC 7 cut(s) 281, 467, 1363, 2004, 2233, 2396, 2850
PsuI RGATCY 4 cut(s) 279, 1204, 2583, 2848
PvuII CAGCTG 1 cut(s) 2093
RsaI GTAC 6 cut(s) 1496, 1874, 1883, 1943, 2213, 2423
RsaNI GTAC 6 cut(s) 1495, 1873, 1882, 1942, 2212, 2422
RseI CAYNNNNRTG 4 cut(s) 99, 1811, 1904, 2628
SapI GCTCTTC 2 cut(s) 2815, 2839
SatI GCNGC 7 cut(s) 243, 624, 642, 852, 1385, 2664, 2667
Sau3AI GATC 9 cut(s) 36, 196, 279, 989, 1038, 1204, 2583, 2824, 2848
SchI GAGTC 3 cut(s) 1792, 2061, 2367
ScrFI CCNGG 2 cut(s) 2829, 2887
SduI GDGCHC 5 cut(s) 22, 106, 589, 2612, 2645
SfaNI GCATC 7 cut(s) 448, 823, 927, 1423, 1759, 2149, 2212
SfcI CTRYAG 3 cut(s) 534, 1136, 1525
SmiMI CAYNNNNRTG 4 cut(s) 99, 1811, 1904, 2628
SmlI CTYRAG 2 cut(s) 1412, 2111
SmoI CTYRAG 2 cut(s) 1412, 2111
SsiI CCGC 6 cut(s) 242, 1385, 2138, 2235, 2743, 2846
SspMI CTAG 9 cut(s) 246, 627, 897, 1218, 1575, 1868, 2045, 2352, 2868
StyD4I CCNGG 2 cut(s) 2827, 2885
StyI CCWWGG 4 cut(s) 1034, 1918, 1945, 2867
TaiI ACGT 6 cut(s) 948, 1428, 1606, 1780, 2423, 2691
TaqI TCGA 7 cut(s) 410, 789, 952, 1686, 1743, 2055, 2507
TatI WGTACW 3 cut(s) 1494, 1872, 2211
TauI GCSGC 2 cut(s) 245, 1387
TfiI GAWTC 7 cut(s) 430, 503, 773, 893, 1159, 1336, 2764
TscAI CASTG 3 cut(s) 46, 1659, 2612
TseFI GTSAC 3 cut(s) 1426, 1505, 1846
TseI GCWGC 5 cut(s) 623, 641, 851, 2663, 2666
Tsp45I GTSAC 3 cut(s) 1426, 1505, 1846
TspGWI ACGGA 3 cut(s) 939, 1746, 2354
TspRI CASTG 3 cut(s) 46, 1659, 2612
Van91I CCANNNNNTGG 2 cut(s) 874, 1658
Vha464I CTTAAG 2 cut(s) 1412, 2111
VneI GTGCAC 1 cut(s) 102
XagI CCTNNNNNAGG 1 cut(s) 438
XapI RAATTY 6 cut(s) 371, 1083, 1221, 1318, 1709, 1819
XbaI TCTAGA 1 cut(s) 2044
XceI RCATGY 4 cut(s) 98, 110, 304, 1440
XcmI CCANNNNNNNNNTGG 2 cut(s) 868, 2577
XmaJI CCTAGG 1 cut(s) 2867
XmiI GTMKAC 2 cut(s) 231, 2655
XmnI GAANNNNTTC 3 cut(s) 743, 1449, 2763
XspI CTAG 9 cut(s) 246, 627, 897, 1218, 1575, 1868, 2045, 2352, 2868
ZraI GACGTC 1 cut(s) 1426
Zsp2I ATGCAT 2 cut(s) 942, 1438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.