RchiOBHm_Chr5g0069741

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000085
Physical Location & Seq
Reverse (-)
75566468 .. 75568321
1854 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34511

Sequence Viewer

Length: 1731 bp
ATGGAAATTGAGTTTTCAATGTCAACCTCAGATTTTGAAGAATTTGAAGCAACAAGACAAGCCATGTCTGAGGTGATGGATGCATTAAAAGATGACACAGTCATTGCCGTTGCGGTGTATGGAATGGGAGGGGTGGGGAAGACAACTATGGTGGAACAGGTGGGTTTACGAGCTCAAAAATGTGGGATTTTCCAACATGTGATTATGGTTGTCATATCCCAAACGCCCAACTTGAGAAATATTCAAGGCACATTGGCAGATCTGCTAGGCTTGGAATTGAAGGAGGAGACAAACGTTGGAAGAGCTACTAGATTGAAGAAGGAGATAATGAGAAGACACAAGATGCTTATAGTCTTGGACGACATTTGGGAGAGAATAGATTTATCAAGCATAGGAATTCCCAGCTACGACCAGCTTCAAAATTGCAATTCCAAAGTCCTACTCACCACAAGGAGATTGAATGTTTGTCATTCCATGGGGACCCAATCAAGCATTCCTCTGAATATCTTATCAGAGGAAGATTCTTGGAACTTGTTTGCGAAGAAAGCAAGAAAACATCTTGAAAAATCAACTGAATTCTATGATGTAGCAAGGAAAGTGGCTAGCGAGTGTGCTGGTCTCCCAATCGCGTTGATAACAGTTGCGAGGGCAGTTGGAGATAAGGATCTTCAGGAATGGAAAGAAGCAGCTGAACGACTTGAGATGGCTCAACCTACTAACCTTGACGATAAGGGAGATGTGTACAAATGTATAAAGTTAAGCTATGACTACTTGCGTGATAATGATGCCAAGTCATGCTTCTTGCTTTGCTGCTTATTCCCAGAAGATGATGATATCCCAATAGAAAGCTTGTTGAAGTATGGGTTAGGGAAAGGATTGTTTCGAGATGTCAACACAAACCAAACAATTCAAAAGGCTAGAGCCAAAATGTACATGGTGGTCAAGTATCTTGTAGCTGCTAACTTGCTTTTGAATGGTATAGGGGATGGATACGTAAGGATGCATGATGTCATTCGAGATATGGCTATGTCAATTGCTTTGTCAGAAGATGGACATGGGTTTTTTGTAAGAACTCGTCGTGAATTAAGAGACTGGCCAATCAATGCAAATGATTGCTACTCAGCAATCTCGCTGATGGAGAACAACATTAGCAAGCTTCCAAAAGAGTTGGTATGTTCAAAACTGCAGATTTTATTGCTAAATAGAAATGCTGATTTGAAAGAGATCTCAGATTCCTTGTTTCAAAGTCCGAATGCATTAAGGGTCTTAGATATTAGTCAAACTCATATTTCATCATTACCCCCATCATTCGGTCTTCTGTCCAATCTTCAAGCTCTCTATGTAGATTATTGCAACTCAAGAATTGACATATCCATACTTACAAAACTGAAGAAACTTGAGATTCTTAGTATGAGAGGATGTGATGTTAAGATATTTCCTATTAAAATAGGACATTTGACCACTTTGAGAATTTTGGACGTCACTGGTGGACGTTTTGATAGAGTTCCATCTCAAGTGATTTCAAAGTTGCATAGATTAGAAGAATTGCGCATGCAATGTCAATTTAAGGGATGGCGTGGTAAAGGCGCGGGAGGCTTTTATTCTGTTCTTTTTTATAAGAATGCTGGCTTCGATGAGTTAACTGGCTTGTCACGTTTAAACCATTTGCATGTATCCCTAGCTGGTTCAGAATGCTTGCCTAAAGCTATCGAGTTTGATCCAAATTGGTAA

Protein Analysis

576

Amino Acids

64.94

Weight (kDa)

6.84

Isoelectric Point (pI)

46.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 20 - 185 3.5e-32 NB-ARC domain
LRR_8 PF13855 394 - 452 1.6e-08 Leucine rich repeat
LRR_14 PF23598 419 - 519 1.5e-11 Leucine-rich repeat region
LRR_14 PF23598 453 - 521 6.8e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1617
AatII GACGTC 1 cut(s) 1482
Acc16I TGCGCA 1 cut(s) 1550
AccII CGCG 2 cut(s) 629, 1589
AciI CCGC 2 cut(s) 113, 1589
AclI AACGTT 1 cut(s) 294
AclWI GGATC 2 cut(s) 672, 1712
AcoI YGGCCR 1 cut(s) 1094
AcsI RAATTY 4 cut(s) 41, 396, 575, 1470
AcuI CTGAAG 2 cut(s) 653, 1409
AcyI GRCGYC 1 cut(s) 1479
AfaI GTAC 2 cut(s) 743, 932
AfiI CCNNNNNNNGG 1 cut(s) 1310
AflIII ACRYGT 1 cut(s) 196
AjuI GAANNNNNNNTTGG 4 cut(s) 477, 509, 782, 814
Alw21I GWGCWC 1 cut(s) 175
Alw26I GTCTC 3 cut(s) 281, 623, 1083
AlwI GGATC 2 cut(s) 672, 1712
AoxI GGCC 1 cut(s) 1094
ApeKI GCWGC 3 cut(s) 686, 810, 956
ApoI RAATTY 4 cut(s) 41, 396, 575, 1470
AspLEI GCGC 2 cut(s) 1551, 1589
AspS9I GGNCC 1 cut(s) 480
AsuHPI GGTGA 2 cut(s) 85, 436
AsuNHI GCTAGC 1 cut(s) 602
AvaII GGWCC 1 cut(s) 480
BalI TGGCCA 1 cut(s) 1096
BanII GRGCYC 1 cut(s) 175
BbsI GAAGAC 3 cut(s) 146, 340, 1307
Bbv12I GWGCWC 1 cut(s) 175
BbvI GCAGC 3 cut(s) 698, 797, 943
BccI CCATC 8 cut(s) 70, 697, 980, 1043, 1129, 1312, 1516, 1566
BceAI ACGGC 1 cut(s) 92
BciVI GTATCC 2 cut(s) 983, 1684
BcoDI GTCTC 3 cut(s) 281, 623, 1083
BfaI CTAG 5 cut(s) 266, 309, 603, 918, 1679
BfmI CTRYAG 1 cut(s) 1184
BfuI GTATCC 2 cut(s) 983, 1684
BglII AGATCT 2 cut(s) 259, 1224
BisI GCNGC 3 cut(s) 687, 811, 957
BlsI GCNGC 3 cut(s) 688, 812, 958
Bme18I GGWCC 1 cut(s) 480
BmgT120I GGNCC 1 cut(s) 480
BmiI GGNNCC 2 cut(s) 481, 482
BmsI GCATC 4 cut(s) 70, 333, 775, 990
BmtI GCTAGC 1 cut(s) 606
BpiI GAAGAC 3 cut(s) 146, 340, 1307
BpuEI CTTGAG 5 cut(s) 253, 719, 1342, 1418, 1497
BsaAI YACGTR 1 cut(s) 994
BsaBI GATNNNNATC 1 cut(s) 663
BsaHI GRCGYC 1 cut(s) 1479
BsaI GGTCTC 1 cut(s) 623
BsaJI CCNNGG 1 cut(s) 474
Bsc4I CCNNNNNNNGG 1 cut(s) 1310
Bse1I ACTGG 3 cut(s) 1097, 1489, 1648
Bse3DI GCAATG 2 cut(s) 102, 1562
Bse8I GATNNNNATC 1 cut(s) 663
BseDI CCNNGG 1 cut(s) 474
BseGI GGATG 5 cut(s) 85, 991, 1005, 1424, 1577
BseJI GATNNNNATC 1 cut(s) 663
BseLI CCNNNNNNNGG 1 cut(s) 1310
BseMI GCAATG 2 cut(s) 102, 1562
BseMII CTCAG 4 cut(s) 42, 60, 1134, 1242
BseNI ACTGG 3 cut(s) 1097, 1489, 1648
BseRI GAGGAG 1 cut(s) 299
BseXI GCAGC 3 cut(s) 698, 797, 943
BseYI CCCAGC 1 cut(s) 401
Bsh1236I CGCG 2 cut(s) 629, 1589
BshFI GGCC 1 cut(s) 1096
BsiHKAI GWGCWC 1 cut(s) 175
BslFI GGGAC 1 cut(s) 493
BslI CCNNNNNNNGG 1 cut(s) 1310
BsmAI GTCTC 3 cut(s) 281, 623, 1083
BsmFI GGGAC 1 cut(s) 493
BsmI GAATGC 4 cut(s) 492, 1258, 1627, 1697
BsnI GGCC 1 cut(s) 1096
Bso31I GGTCTC 1 cut(s) 623
Bsp1286I GDGCHC 1 cut(s) 175
Bsp1407I TGTACA 2 cut(s) 741, 930
Bsp143I GATC 4 cut(s) 259, 664, 1224, 1717
Bsp19I CCATGG 1 cut(s) 474
BspACI CCGC 2 cut(s) 113, 1589
BspANI GGCC 1 cut(s) 1096
BspCNI CTCAG 4 cut(s) 41, 61, 1133, 1241
BspFNI CGCG 2 cut(s) 629, 1589
BspLI GGNNCC 2 cut(s) 481, 482
BspMAI CTGCAG 1 cut(s) 1188
BspOI GCTAGC 1 cut(s) 606
BspPI GGATC 2 cut(s) 672, 1712
BspQI GCTCTTC 1 cut(s) 295
BspTNI GGTCTC 1 cut(s) 623
BsrDI GCAATG 2 cut(s) 102, 1562
BsrGI TGTACA 2 cut(s) 741, 930
BsrI ACTGG 3 cut(s) 1097, 1489, 1648
BssECI CCNNGG 1 cut(s) 474
BssMI GATC 4 cut(s) 259, 664, 1224, 1717
BssNI GRCGYC 1 cut(s) 1479
BssT1I CCWWGG 1 cut(s) 474
Bst4CI ACNGT 2 cut(s) 100, 640
Bst6I CTCTTC 1 cut(s) 295
BstACI GRCGYC 1 cut(s) 1479
BstAUI TGTACA 2 cut(s) 741, 930
BstBAI YACGTR 1 cut(s) 994
BstC8I GCNNGC 5 cut(s) 604, 1154, 1553, 1627, 1697
BstDEI CTNAG 6 cut(s) 28, 69, 1120, 1228, 1267, 1406
BstDSI CCRYGG 1 cut(s) 474
BstF5I GGATG 5 cut(s) 85, 991, 1005, 1424, 1577
BstFNI CGCG 2 cut(s) 629, 1589
BstHHI GCGC 2 cut(s) 1551, 1589
BstKTI GATC 4 cut(s) 262, 667, 1227, 1720
BstMAI GTCTC 3 cut(s) 281, 623, 1083
BstMBI GATC 4 cut(s) 259, 664, 1224, 1717
BstMWI GCNNNNNNNGC 2 cut(s) 545, 1593
BstNSI RCATGY 3 cut(s) 200, 1555, 1673
BstSFI CTRYAG 1 cut(s) 1184
BstSNI TACGTA 1 cut(s) 994
BstUI CGCG 2 cut(s) 629, 1589
BstV1I GCAGC 3 cut(s) 698, 797, 943
BstV2I GAAGAC 3 cut(s) 146, 340, 1307
BstX2I RGATCY 3 cut(s) 259, 664, 1224
BstYI RGATCY 3 cut(s) 259, 664, 1224
BsuI GTATCC 2 cut(s) 983, 1684
BsuRI GGCC 1 cut(s) 1096
BtgI CCRYGG 1 cut(s) 474
BtsCI GGATG 5 cut(s) 85, 991, 1005, 1424, 1577
BtsIMutI CAGTG 1 cut(s) 1482
Cac8I GCNNGC 5 cut(s) 604, 1154, 1553, 1627, 1697
CfoI GCGC 2 cut(s) 1551, 1589
Cfr13I GGNCC 1 cut(s) 480
Csp6I GTAC 2 cut(s) 742, 931
CspCI CAANNNNNGTGG 4 cut(s) 132, 167, 579, 614
CviAII CATG 9 cut(s) 64, 197, 475, 795, 934, 1004, 1055, 1552, 1670
CviQI GTAC 2 cut(s) 742, 931
DdeI CTNAG 6 cut(s) 28, 69, 1120, 1228, 1267, 1406
DpnI GATC 4 cut(s) 261, 666, 1226, 1719
DpnII GATC 4 cut(s) 259, 664, 1224, 1717
DraI TTTAAA 1 cut(s) 1659
EaeI YGGCCR 1 cut(s) 1094
Eam1104I CTCTTC 1 cut(s) 295
EarI CTCTTC 1 cut(s) 295
Ecl136II GAGCTC 1 cut(s) 173
Eco105I TACGTA 1 cut(s) 994
Eco130I CCWWGG 1 cut(s) 474
Eco24I GRGCYC 1 cut(s) 175
Eco31I GGTCTC 1 cut(s) 623
Eco32I GATATC 1 cut(s) 835
Eco47I GGWCC 1 cut(s) 480
Eco53kI GAGCTC 1 cut(s) 173
Eco57I CTGAAG 2 cut(s) 653, 1409
EcoICRI GAGCTC 1 cut(s) 173
EcoO109I RGGNCCY 1 cut(s) 480
EcoRI GAATTC 2 cut(s) 396, 575
EcoRV GATATC 1 cut(s) 835
EcoT14I CCWWGG 1 cut(s) 474
EcoT22I ATGCAT 3 cut(s) 85, 1005, 1258
EcoT38I GRGCYC 1 cut(s) 175
ErhI CCWWGG 1 cut(s) 474
FaeI CATG 9 cut(s) 67, 200, 478, 798, 937, 1007, 1058, 1555, 1673
FalI AAGNNNNNCTT 2 cut(s) 782, 814
FaqI GGGAC 1 cut(s) 493
FatI CATG 9 cut(s) 63, 196, 474, 794, 933, 1003, 1054, 1551, 1669
FauI CCCGC 1 cut(s) 1582
Fnu4HI GCNGC 3 cut(s) 687, 811, 957
FokI GGATG 5 cut(s) 92, 998, 1012, 1431, 1584
FriOI GRGCYC 1 cut(s) 175
Fsp4HI GCNGC 3 cut(s) 687, 811, 957
FspBI CTAG 5 cut(s) 266, 309, 603, 918, 1679
FspI TGCGCA 1 cut(s) 1550
GlaI GCGC 2 cut(s) 1550, 1588
GluI GCNGC 3 cut(s) 687, 811, 957
GsaI CCCAGC 1 cut(s) 405
HaeIII GGCC 1 cut(s) 1096
HhaI GCGC 2 cut(s) 1551, 1589
Hin1I GRCGYC 1 cut(s) 1479
Hin1II CATG 9 cut(s) 67, 200, 478, 798, 937, 1007, 1058, 1555, 1673
Hin6I GCGC 2 cut(s) 1549, 1587
HinP1I GCGC 2 cut(s) 1549, 1587
HincII GTYRAC 3 cut(s) 24, 892, 1641
HindII GTYRAC 3 cut(s) 24, 892, 1641
HindIII AAGCTT 2 cut(s) 847, 1154
HinfI GANTC 3 cut(s) 521, 1232, 1402
HpaI GTTAAC 1 cut(s) 1641
HphI GGTGA 2 cut(s) 85, 436
Hpy166II GTNNAC 6 cut(s) 24, 167, 742, 892, 1490, 1641
Hpy188I TCNGA 8 cut(s) 31, 70, 501, 514, 1045, 1231, 1251, 1690
Hpy188III TCNNGA 6 cut(s) 560, 671, 884, 1016, 1079, 1359
Hpy8I GTNNAC 6 cut(s) 24, 167, 742, 892, 1490, 1641
Hpy99I CGWCG 1 cut(s) 1080
HpyAV CCTTC 2 cut(s) 274, 313
HpyCH4III ACNGT 2 cut(s) 100, 640
HpyCH4IV ACGT 5 cut(s) 294, 993, 1479, 1492, 1654
HpyF10VI GCNNNNNNNGC 2 cut(s) 545, 1593
HpyF3I CTNAG 6 cut(s) 28, 69, 1120, 1228, 1267, 1406
HpySE526I ACGT 5 cut(s) 294, 993, 1479, 1492, 1654
Hsp92I GRCGYC 1 cut(s) 1479
Hsp92II CATG 9 cut(s) 67, 200, 478, 798, 937, 1007, 1058, 1555, 1673
HspAI GCGC 2 cut(s) 1549, 1587
KflI GGGWCCC 1 cut(s) 480
KspAI GTTAAC 1 cut(s) 1641
Kzo9I GATC 4 cut(s) 259, 664, 1224, 1717
LguI GCTCTTC 1 cut(s) 295
Lsp1109I GCAGC 3 cut(s) 698, 797, 943
LweI GCATC 4 cut(s) 70, 333, 775, 990
MaeI CTAG 5 cut(s) 266, 309, 603, 918, 1679
MaeII ACGT 5 cut(s) 294, 993, 1479, 1492, 1654
MaeIII GTNAC 2 cut(s) 1480, 1650
MalI GATC 4 cut(s) 261, 666, 1226, 1719
MboI GATC 4 cut(s) 259, 664, 1224, 1717
MfeI CAATTG 1 cut(s) 1032
MflI RGATCY 3 cut(s) 259, 664, 1224
MhlI GDGCHC 1 cut(s) 175
MlsI TGGCCA 1 cut(s) 1096
MluNI TGGCCA 1 cut(s) 1096
MmeI TCCRAC 3 cut(s) 217, 277, 634
MnlI CCTC 9 cut(s) 37, 64, 122, 277, 507, 508, 639, 1409, 1586
Mox20I TGGCCA 1 cut(s) 1096
Mph1103I ATGCAT 3 cut(s) 85, 1005, 1258
MscI TGGCCA 1 cut(s) 1096
MseI TTAA 9 cut(s) 86, 758, 1085, 1259, 1428, 1443, 1566, 1640, 1658
MslI CAYNNNNRTG 1 cut(s) 1668
Msp20I TGGCCA 1 cut(s) 1096
MspA1I CMGCKG 1 cut(s) 689
MssI GTTTAAAC 1 cut(s) 1659
MunI CAATTG 1 cut(s) 1032
Mva1269I GAATGC 4 cut(s) 492, 1258, 1627, 1697
MvnI CGCG 2 cut(s) 629, 1589
MwoI GCNNNNNNNGC 2 cut(s) 545, 1593
NcoI CCATGG 1 cut(s) 474
NdeII GATC 4 cut(s) 259, 664, 1224, 1717
NheI GCTAGC 1 cut(s) 602
NlaIII CATG 9 cut(s) 67, 200, 478, 798, 937, 1007, 1058, 1555, 1673
NlaIV GGNNCC 2 cut(s) 481, 482
NmuCI GTSAC 2 cut(s) 1480, 1650
NsbI TGCGCA 1 cut(s) 1550
NsiI ATGCAT 3 cut(s) 85, 1005, 1258
NspI RCATGY 3 cut(s) 200, 1555, 1673
PaeI GCATGC 1 cut(s) 1555
PciI ACATGT 1 cut(s) 196
PciSI GCTCTTC 1 cut(s) 295
PctI GAATGC 4 cut(s) 492, 1258, 1627, 1697
PfeI GAWTC 3 cut(s) 521, 1232, 1402
PflFI GACNNNGTC 1 cut(s) 98
PkrI GCNGC 3 cut(s) 688, 812, 958
PmeI GTTTAAAC 1 cut(s) 1659
Ppu21I YACGTR 1 cut(s) 994
PpuMI RGGWCCY 1 cut(s) 480
PscI ACATGT 1 cut(s) 196
PsiI TTATAA 1 cut(s) 1617
Psp124BI GAGCTC 1 cut(s) 175
Psp1406I AACGTT 1 cut(s) 294
Psp5II RGGWCCY 1 cut(s) 480
PspFI CCCAGC 1 cut(s) 401
PspN4I GGNNCC 2 cut(s) 481, 482
PspPI GGNCC 1 cut(s) 480
PspPPI RGGWCCY 1 cut(s) 480
PstI CTGCAG 1 cut(s) 1188
PsuI RGATCY 3 cut(s) 259, 664, 1224
PsyI GACNNNGTC 1 cut(s) 98
PvuII CAGCTG 1 cut(s) 689
RsaI GTAC 2 cut(s) 743, 932
RsaNI GTAC 2 cut(s) 742, 931
RseI CAYNNNNRTG 1 cut(s) 1668
SacI GAGCTC 1 cut(s) 175
SapI GCTCTTC 1 cut(s) 295
SaqAI TTAA 9 cut(s) 86, 758, 1085, 1259, 1428, 1443, 1566, 1640, 1658
SatI GCNGC 3 cut(s) 687, 811, 957
Sau3AI GATC 4 cut(s) 259, 664, 1224, 1717
Sau96I GGNCC 1 cut(s) 480
SduI GDGCHC 1 cut(s) 175
SfaNI GCATC 4 cut(s) 70, 333, 775, 990
SfcI CTRYAG 1 cut(s) 1184
SinI GGWCC 1 cut(s) 480
SmiMI CAYNNNNRTG 1 cut(s) 1668
SmlI CTYRAG 5 cut(s) 232, 698, 1357, 1397, 1512
SmoI CTYRAG 5 cut(s) 232, 698, 1357, 1397, 1512
SnaBI TACGTA 1 cut(s) 994
SphI GCATGC 1 cut(s) 1555
SsiI CCGC 2 cut(s) 113, 1589
SspI AATATT 1 cut(s) 241
SspMI CTAG 5 cut(s) 266, 309, 603, 918, 1679
SstI GAGCTC 1 cut(s) 175
StyI CCWWGG 1 cut(s) 474
TaaI ACNGT 2 cut(s) 100, 640
TaiI ACGT 5 cut(s) 297, 996, 1482, 1495, 1657
TaqI TCGA 4 cut(s) 883, 1015, 1632, 1710
TaqII GACCGA 1 cut(s) 1301
TatI WGTACW 2 cut(s) 741, 930
TfiI GAWTC 3 cut(s) 521, 1232, 1402
Tru1I TTAA 9 cut(s) 86, 758, 1085, 1259, 1428, 1443, 1566, 1640, 1658
Tru9I TTAA 9 cut(s) 86, 758, 1085, 1259, 1428, 1443, 1566, 1640, 1658
TscAI CASTG 1 cut(s) 1489
TseFI GTSAC 2 cut(s) 1480, 1650
TseI GCWGC 3 cut(s) 686, 810, 956
Tsp45I GTSAC 2 cut(s) 1480, 1650
TspDTI ATGAA 1 cut(s) 1281
TspRI CASTG 1 cut(s) 1489
Tth111I GACNNNGTC 1 cut(s) 98
VpaK11BI GGWCC 1 cut(s) 480
XapI RAATTY 4 cut(s) 41, 396, 575, 1470
XceI RCATGY 3 cut(s) 200, 1555, 1673
XcmI CCANNNNNNNNNTGG 1 cut(s) 931
XspI CTAG 5 cut(s) 266, 309, 603, 918, 1679
ZraI GACGTC 1 cut(s) 1480
Zsp2I ATGCAT 3 cut(s) 85, 1005, 1258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.