RchiOBHm_Chr5g0064111

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000085
Physical Location & Seq
Forward (+)
69937203 .. 69942799
5597 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34017

Sequence Viewer

Length: 4359 bp
ATGGTCACATTGTCCTACGACCTCTTTTCTTTTCAGTGTGGTGTCACTGGTGTGGTTTCTGCAATCTGCTTAGTTTGCTTCATCATGACTCTGTCTTTCCTTTTTTGTTCTTCTCGTTCTCGATCAAAGCCTTCGAGCAATGCAAGTGCTTCTGCATCTCATCCCTTACCTGTTAGACCAGATCCTCAGCTCTTGGAAGATTCTGAATCAAAGCAACCTAAGGATGATCTAGTACGCTTCCATTTCTTGAAGCAACCCAAGCCATGGATCACTTACACAAGCAGTTCTAAAACAGCCTGGAAGCATGATGTGTTTTTGAGCTTTAGGGGTGAAGACACCCGCCATGGTTTCGTATCTCATTTATACGATGAATTGGAAAGCACCGGAAGAATTAAAACATTCAAGGATGACCAAGGGCTGCAAGCAGGGATGTCAATTTCTCCAAGTCTCCTCAGGGCAATTGAAGAATCAAAGCTGGCAATTGTTGTTTTGTCGCCAAACTACGCTTCTTCTCCATGGTGTTTGGATGAGCTTACACATATATTTCAATTCATGGAAGGCAGAGACAGAATTCTGCCAATTTTTTATCATGTGGATCCAGCTGATGTACGACATCAAAGGGGGAGTTTTGCAACAGCTTTCACTAATTATGAAAGAAAGTCTAGGCAAAGCGCAGAAAAGTTGTATCAGTGGAGAGCTGCCTTAAGACAAGTGGCCAATATCTCTGGGTGGAATACAAAGAACTTTGGGTCCGAGAGTGAGCTTGTCAAACGCATTGCGAAAGATGTCAGCAGTAAAACAACACTTGAAGTTCAACAACGGCCCGTCGAAATTGATATTACCATCCCGTCCACCCGAGAGTTTAGAATATTTGAATCAACAAGACAGGCCATGGATGAGCTGATTAAGGCGCTGATAGATGATGAGGTCACCATTTCTGGGGTTTATGGCATGGGAGGTGTGGGCAAGACAACCATGGTGAGAAATGTTGCTCTACAAGCACGAAAAAATGGGCTCTTTGATCATGTGATTGTGGCTGTCGTATCCCAAGACCCCAACGTTAGAAGGATTCAACAGCAATTGGCTGAGCAGCTCGACTTGAGATTGGAGGGGACGGAATCTGAAACAGCCGATAGATTGAAGGAAAGGATAATGGAAGGAAAGAGGATCCTTATAATCTTGGACGACATTTGGGAAAGCATAGACTTGTCACTTATAGGAATTCCCAACCCCGATGAGCTTAAGCGATGCAACTCTAAAGTCCTAATCACCACAAGAAGATTGGATGTTTGCCGCTCCATGAAGAGCCAAGCAAGTTTTCTTCTCAGTATATTATCTAAAGAAGATTCTTGGAAACTTTTTGTGGATTGTTCGAGAATATCTTTTGATGAATCTACCAATATCTATCGAGCGAGGGCGATAGTTAGAGAATGTGGTGGTCTGCCCGTTGCATTGAAAGCAATTGCACGGGCACTTGAAGATGAAGACATGGCTGAATGGAATATAGCAGTTCAACAATTGAGGGCATCTCAAATTGCCTGTCTTGATGATCAGGGTCCTGCGTTCAAATGTATAAAGTTAGGCTATGACTACTTGAGATCTGATGATGCCAAATCATTCTTCTTGCTTTGCTCCCTATTCCCAGAAGACTATGATATCCCTATCAAAGACTTGTTCAAGTATGCTATCGGGAAAGGATTGCTTCGAGAAGCCGCCACAATCCAAGAAGCCAGAGATAGAGCAAATTCGGTGATCAAATACCTCAAAGCTTCCAGCTTACTTTTGGACAGTCCAAAGGAGGGATGCGTAAAGATGCATGATGTTGTTCGGGATACAGCCAAGTCAATTCCAAGGTATGAGGATGATCATGGATTTTGCGTTAAAGCTGGTTGTGGATTAAAGGAGTTTCGAGGGACCACTGGGCATGGAGACTGCTCTGCCATCTCACTAATGATGAATGATATTGATCGGCTTCCTGAAGAGTTGGTATGTCCAAAACTTCAGATTTTGTTACTACAAAACAATGTTCATATAGATGAGATCCCAGAATCTTTTTTTCGAAGTTCGAAGGCACTAAGGGTCTTAGATATTAGCTTCTCTAGAATTTCATCACTACCCAGGTCATTTAGTTTCCTAATCAACCTCAGAGCTTTGTTTTTAGACCATTGCAATGAGTTGGTTGACATATCAATATTCGGAAACTTGAAGAGACTTGAGATTCTTAGTATGATAGAATTTCCTCTCAAGGAGTTACCAAAAGAGATGGGAAATTTGACCAATCTAAGGATGCTGGACTTAAGTGGGAGTTACAATATTGGTATATTTCCATATAGTGTGCTGTCAAGATTGTGTAAATTAGAAGAACTGTACATGCAATGTAATTCTGGGAACTGGGGAAGCAAAATTCATGGAGGAGAAGAAACTAATGTTGGTTCTGATAAGTTGATTGGCTTGCCACTTTTAAACACCGTGAAGCTTCGCATATCTGATGCAGAATGCTTGCCTAAAAATCTGTTCTGGGATTGGCATTTGGTTACCTTTGATATATGTATCAGCAGAGAACCACCATCTGGAGACTACTCTTCAGAACCAATTTTCTCACACGATCATTCAAGGACCCTGACTCTTAACACAACAATCAACAGCTTACCAGATTGGTTTATCAACACAGTGACAGAGAAAGCAGTGAAACTACAGTACCTAAAGTGCAGGGGATTAAAGAACATTCTTGTGGAACATAACAGAGGCAGATTACATGGACTGAAGAATCTTGCTGTAATTGGTCCACATGAGAACTTGGAAGTGTTGATGGAAACAATAACACTGGTGCCGAATAAACCTGTGTTTGAGAAGTTGGAAGAGTTGCATCTCCTTGGTGTGAGTTGCCTGAAGGAATTATGTGTCGGTGAATTACCCGTTGGGTCTCTATGCAATCTCAAGTTATTCAAGGTAGAATATTGCCATGACTTGGTAAAGACACTCTTACCATCAAATTTGTGGCAGAGACTACACAATATGGAAAAACTAATTTGTGAAGATATGAATCTAATGGAGTATGTATTTGGATCCAAAGGGATGGAGTCTGAAGATATTATCTTGATAAAATTGAGAGAGATGATATTGTGGAATCTAGAGAATCTAATAGGCATATGCAATGGTCCTGCTCCAAATGCAGTCTTCCATAGTCTTAAAAGTTTGGCAGTATATGGGTGCAAGAAACTGAAACATCTGTTCACATACGATATAGCTCACTGTCTTTTGCATTTGGAAGACCTTTCGGTGACTCAGTGTTCCAGCTTGGACAGGGTTATCGAACCTAGCAAGGAAACAGTCAACAAGAAGATGGTTTTTCCTGAATTGAAAAACTTAGCTTTGTCATATCTTCCGCAGCTCACAAGGTTCTGCAGTAGTGAAAGTGCTACCATTGAGTGCCCTTCATTGGAACACTTGCATGTGCAGGACTGCCCCCATTTTACAACCTCTATTTCTGACTTCCACAGTGGGAATCGATTCATCCAAGTCAATGATCTACGACATTTGAGGATGAGCAAAGACTTGAATAGCATCAATTTAACTGGCCAGCACACCATGGACATCCTTGAGAGCCTACCTCATAATGTCAGAAAGCGGGTTGAGTTCATGAGAGAAATTGAGAGTCAACGTGATGAATTAGAGGCCAAGCTCTTCAAGGAGAAAGCTGAACTTGAAGCCAAGTATCAGAAATTATATCAGAATCTCTATAGCAAGCGGTACTTGATTGTGAATGGTGTTGTTGAAACTGAAGGAGTTGCCACTGAAACAATAACAGATCATGAGAACAAAACTGCAAAAGAGAGAGGAGTGCCTGATTTCTGGCTGAATGCATTGAAGAATAATGCAGTGCTAGCCAAGGAGATTACCGAGTGTGATGAAGGAGCTCTTAAATATCTTAGGGACGTCAAGTGTTTTAAGATACATAATTCGAAGGGGTTCAAGCTTGAGTTCGACTTTGACACCAATCCATTTTTCAAAAACTCTGTTTTGACAAAGACATATCACATGATTGATGAAGATGAACCAAACAGCACAGCAGGGACGGAGATTGAATGGTATCCAGCAAAATGCCTGACACAGAAGATTGTTAAGAAGAAGCATAAGAAGGGATCAAAGAATTCTAAGCTTCTGACTAAAACTAAAAATTATAAAAGTTTCTTCAATTTCTTTAGCCCACATCAAGTCTCCAAGGACCATGAAGTTATTGATGAAGCTGCTGCCGAGGAACTCAAAAACCGGAAGAAACAAGACTATGATGTTGGCTCAACCATTTGTTATAAGATCATCCCACATGCAGTTTCGTGGTATACTGGAGAAGCTTTTTAG

Protein Analysis

1452

Amino Acids

165.27

Weight (kDa)

6.58

Isoelectric Point (pI)

41.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 101 - 274 1.6e-51 TIR domain
TIR_2 PF13676 104 - 203 1e-16 TIR domain
NB-ARC PF00931 296 - 455 3.4e-34 NB-ARC domain
AAA_22 PF13401 315 - 397 8.2e-06 AAA domain
LRR_8 PF13855 665 - 724 5.6e-09 Leucine rich repeat
LRR_14 PF23598 667 - 794 2.4e-11 Leucine-rich repeat region
LRR_RPS2 PF23247 952 - 1097 1.8e-26 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1055 - 1160 2.2e-16 Plant disease resistance protein RPS2-like, leucine-rich repeats
NAP PF00956 1209 - 1450 6.4e-72 Nucleosome assembly protein (NAP)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1175, 4182, 4311
AatII GACGTC 1 cut(s) 3939
AccB1I GGYRCC 1 cut(s) 2827
AccB7I CCANNNNNTGG 3 cut(s) 264, 2570, 2968
AccBSI CCGCTC 1 cut(s) 1296
AccI GTMKAC 1 cut(s) 4340
AciI CCGC 6 cut(s) 340, 1294, 1713, 3386, 3628, 3748
AclI AACGTT 1 cut(s) 1059
AcoI YGGCCR 2 cut(s) 714, 3577
AcsI RAATTY 9 cut(s) 570, 1221, 1744, 2103, 2234, 2269, 2403, 2992, 4150
AcuI CTGAAG 7 cut(s) 1985, 1998, 2568, 2783, 2909, 3105, 3801
AcyI GRCGYC 1 cut(s) 3936
AfaI GTAC 5 cut(s) 234, 609, 2369, 2699, 3752
AfiI CCNNNNNNNGG 7 cut(s) 264, 939, 1799, 2283, 2570, 2968, 3439
AflII CTTAAG 3 cut(s) 703, 1241, 2296
AjnI CCWGG 2 cut(s) 296, 2117
AleI CACNNNNGTG 1 cut(s) 50
AloI GAACNNNNNNTCC 2 cut(s) 734, 766
Alw21I GWGCWC 1 cut(s) 3919
Alw26I GTCTC 8 cut(s) 452, 558, 1923, 2203, 2568, 2928, 2998, 4222
Ama87I CYCGRG 1 cut(s) 855
AoxI GGCC 5 cut(s) 714, 821, 888, 3577, 3675
ApeKI GCWGC 6 cut(s) 418, 698, 1090, 3388, 4247, 4250
ApoI RAATTY 9 cut(s) 570, 1221, 1744, 2103, 2234, 2269, 2403, 2992, 4150
ArsI GACNNNNNNTTYG 2 cut(s) 1525, 1557
Asp700I GAANNNNTTC 2 cut(s) 3509, 3968
AspLEI GCGC 2 cut(s) 674, 913
AspS9I GGNCC 8 cut(s) 750, 822, 1556, 1914, 2616, 2783, 3158, 4225
AsuHPI GGTGA 7 cut(s) 341, 922, 991, 1261, 1762, 2918, 3292
AsuII TTCGAA 3 cut(s) 2059, 2066, 3962
AsuNHI GCTAGC 1 cut(s) 3883
AvaI CYCGRG 1 cut(s) 855
AvaII GGWCC 7 cut(s) 750, 1556, 1914, 2616, 2783, 3158, 4225
AxyI CCTNAGG 2 cut(s) 219, 452
BaeGI GKGCMC 2 cut(s) 1474, 3434
BaeI ACNNNNGTAYC 2 cut(s) 2681, 2714
BalI TGGCCA 2 cut(s) 716, 3579
BamHI GGATCC 3 cut(s) 595, 1167, 3065
BanI GGYRCC 1 cut(s) 2827
BanII GRGCYC 2 cut(s) 1017, 3919
BbsI GAAGAC 5 cut(s) 339, 1491, 1653, 3169, 3276
Bbv12I GWGCWC 1 cut(s) 3919
BbvCI CCTCAGC 1 cut(s) 186
BbvI GCAGC 6 cut(s) 405, 685, 1102, 3400, 4234, 4237
BccI CCATC 8 cut(s) 851, 1949, 2257, 2575, 2803, 2995, 3070, 3337
BceAI ACGGC 1 cut(s) 836
BciT130I CCWGG 2 cut(s) 298, 2119
BciVI GTATCC 3 cut(s) 1054, 1825, 4101
BclI TGATCA 4 cut(s) 1021, 1549, 1752, 1864
BcoDI GTCTC 8 cut(s) 452, 558, 1923, 2203, 2568, 2928, 2998, 4222
BfaI CTAG 6 cut(s) 230, 663, 2100, 3131, 3318, 3884
BfmI CTRYAG 3 cut(s) 2693, 3403, 3739
BfoI RGCGCY 1 cut(s) 914
BfrI CTTAAG 3 cut(s) 703, 1241, 2296
BfuI GTATCC 3 cut(s) 1054, 1825, 4101
BglII AGATCT 1 cut(s) 1598
BisI GCNGC 8 cut(s) 419, 699, 1091, 1294, 1713, 3389, 4248, 4251
BlpI GCTNAGC 1 cut(s) 1086
BlsI GCNGC 8 cut(s) 420, 700, 1092, 1295, 1714, 3390, 4249, 4252
Bme1390I CCNGG 2 cut(s) 298, 2119
Bme18I GGWCC 7 cut(s) 750, 1556, 1914, 2616, 2783, 3158, 4225
BmeT110I CYCGRG 1 cut(s) 855
BmgT120I GGNCC 8 cut(s) 750, 822, 1556, 1914, 2616, 2783, 3158, 4225
BmiI GGNNCC 8 cut(s) 597, 751, 1169, 1557, 1915, 2618, 2829, 3067
BmrFI CCNGG 2 cut(s) 298, 2119
BmrI ACTGGG 2 cut(s) 1929, 2401
BmtI GCTAGC 1 cut(s) 3887
BmuI ACTGGG 2 cut(s) 1929, 2401
BpiI GAAGAC 5 cut(s) 339, 1491, 1653, 3169, 3276
BplI GAGNNNNNCTC 4 cut(s) 1513, 1545, 3595, 3627
BpmI CTGGAG 1 cut(s) 2592
Bpu10I CCTNAGC 1 cut(s) 186
Bpu1102I GCTNAGC 1 cut(s) 1086
Bpu14I TTCGAA 3 cut(s) 2059, 2066, 3962
BpuEI CTTGAG 7 cut(s) 1120, 1615, 2228, 2234, 2921, 3620, 3998
Bsa29I ATCGAT 1 cut(s) 3508
BsaBI GATNNNNATC 2 cut(s) 1965, 3042
BsaHI GRCGYC 1 cut(s) 3936
BsaI GGTCTC 1 cut(s) 2928
BsaWI WCCGGW 2 cut(s) 383, 4269
Bsc4I CCNNNNNNNGG 7 cut(s) 264, 939, 1799, 2283, 2570, 2968, 3439
Bse1I ACTGG 6 cut(s) 52, 1924, 2396, 2829, 3580, 4348
Bse21I CCTNAGG 2 cut(s) 219, 452
Bse3DI GCAATG 6 cut(s) 145, 774, 2164, 2176, 2381, 3160
Bse8I GATNNNNATC 2 cut(s) 1965, 3042
BseBI CCWGG 2 cut(s) 298, 2119
BseCI ATCGAT 1 cut(s) 3508
BseJI GATNNNNATC 2 cut(s) 1965, 3042
BseLI CCNNNNNNNGG 7 cut(s) 264, 939, 1799, 2283, 2570, 2968, 3439
BseMI GCAATG 6 cut(s) 145, 774, 2164, 2176, 2381, 3160
BseMII CTCAG 6 cut(s) 200, 466, 1077, 1339, 2158, 3299
BseNI ACTGG 6 cut(s) 52, 1924, 2396, 2829, 3580, 4348
BseRI GAGGAG 3 cut(s) 440, 2427, 3852
BseSI GKGCMC 2 cut(s) 1474, 3434
BseXI GCAGC 6 cut(s) 405, 685, 1102, 3400, 4234, 4237
BsgI GTGCAG 2 cut(s) 2728, 3476
BshFI GGCC 5 cut(s) 716, 823, 890, 3579, 3677
BshNI GGYRCC 1 cut(s) 2827
BshVI ATCGAT 1 cut(s) 3508
BsiHKAI GWGCWC 1 cut(s) 3919
BsiHKCI CYCGRG 1 cut(s) 855
BsiSI CCGG 2 cut(s) 384, 4270
BslFI GGGAC 4 cut(s) 1126, 1927, 3947, 4087
BslI CCNNNNNNNGG 7 cut(s) 264, 939, 1799, 2283, 2570, 2968, 3439
BsmAI GTCTC 8 cut(s) 452, 558, 1923, 2203, 2568, 2928, 2998, 4222
BsmFI GGGAC 4 cut(s) 1126, 1927, 3947, 4087
BsmI GAATGC 2 cut(s) 2501, 3865
BsnI GGCC 5 cut(s) 716, 823, 890, 3579, 3677
Bso31I GGTCTC 1 cut(s) 2928
BsoBI CYCGRG 1 cut(s) 855
Bsp119I TTCGAA 3 cut(s) 2059, 2066, 3962
Bsp1286I GDGCHC 4 cut(s) 1017, 1474, 3434, 3919
Bsp1407I TGTACA 1 cut(s) 2367
Bsp1720I GCTNAGC 1 cut(s) 1086
Bsp19I CCATGG 6 cut(s) 263, 343, 515, 891, 975, 3588
BspACI CCGC 6 cut(s) 340, 1294, 1713, 3386, 3628, 3748
BspANI GGCC 5 cut(s) 716, 823, 890, 3579, 3677
BspCNI CTCAG 6 cut(s) 199, 465, 1078, 1338, 2157, 3298
BspDI ATCGAT 1 cut(s) 3508
BspHI TCATGA 3 cut(s) 84, 3639, 3811
BspLI GGNNCC 8 cut(s) 597, 751, 1169, 1557, 1915, 2618, 2829, 3067
BspMAI CTGCAG 1 cut(s) 3407
BspOI GCTAGC 1 cut(s) 3887
BspQI GCTCTTC 2 cut(s) 1298, 3689
BspT104I TTCGAA 3 cut(s) 2059, 2066, 3962
BspT107I GGYRCC 1 cut(s) 2827
BspTI CTTAAG 3 cut(s) 703, 1241, 2296
BspTNI GGTCTC 1 cut(s) 2928
BsrBI CCGCTC 1 cut(s) 1296
BsrDI GCAATG 6 cut(s) 145, 774, 2164, 2176, 2381, 3160
BsrGI TGTACA 1 cut(s) 2367
BsrI ACTGG 6 cut(s) 52, 1924, 2396, 2829, 3580, 4348
BssNAI GTATAC 1 cut(s) 4341
BssNI GRCGYC 1 cut(s) 3936
Bst1107I GTATAC 1 cut(s) 4341
Bst2UI CCWGG 2 cut(s) 298, 2119
Bst4CI ACNGT 8 cut(s) 1790, 2367, 2470, 2671, 2697, 3254, 3331, 3500
Bst6I CTCTTC 6 cut(s) 1298, 1974, 2201, 2587, 2853, 3689
BstACI GRCGYC 1 cut(s) 3936
BstAFI CTTAAG 3 cut(s) 703, 1241, 2296
BstAUI TGTACA 1 cut(s) 2367
BstBI TTCGAA 3 cut(s) 2059, 2066, 3962
BstC8I GCNNGC 7 cut(s) 423, 477, 2453, 2501, 3581, 3746, 3885
BstDSI CCRYGG 6 cut(s) 263, 343, 515, 891, 975, 3588
BstEII GGTNACC 2 cut(s) 928, 2533
BstH2I RGCGCY 1 cut(s) 914
BstHHI GCGC 2 cut(s) 674, 913
BstMAI GTCTC 8 cut(s) 452, 558, 1923, 2203, 2568, 2928, 2998, 4222
BstMWI GCNNNNNNNGC 6 cut(s) 75, 259, 998, 1457, 3170, 3884
BstNI CCWGG 2 cut(s) 298, 2119
BstNSI RCATGY 3 cut(s) 2374, 3455, 4328
BstPI GGTNACC 2 cut(s) 928, 2533
BstSCI CCNGG 2 cut(s) 296, 2117
BstSFI CTRYAG 3 cut(s) 2693, 3403, 3739
BstSLI GKGCMC 2 cut(s) 1474, 3434
BstV1I GCAGC 6 cut(s) 405, 685, 1102, 3400, 4234, 4237
BstV2I GAAGAC 5 cut(s) 339, 1491, 1653, 3169, 3276
BstX2I RGATCY 6 cut(s) 181, 595, 1167, 1598, 2040, 3065
BstXI CCANNNNNNTGG 1 cut(s) 3076
BstYI RGATCY 6 cut(s) 181, 595, 1167, 1598, 2040, 3065
BstZ17I GTATAC 1 cut(s) 4341
Bsu15I ATCGAT 1 cut(s) 3508
Bsu36I CCTNAGG 2 cut(s) 219, 452
BsuI GTATCC 3 cut(s) 1054, 1825, 4101
BsuRI GGCC 5 cut(s) 716, 823, 890, 3579, 3677
BsuTUI ATCGAT 1 cut(s) 3508
BtgI CCRYGG 6 cut(s) 263, 343, 515, 891, 975, 3588
BtgZI GCGATG 1 cut(s) 1261
BtsI GCAGTG 2 cut(s) 2691, 3885
Cac8I GCNNGC 7 cut(s) 423, 477, 2453, 2501, 3581, 3746, 3885
CciI TCATGA 3 cut(s) 84, 3639, 3811
CfoI GCGC 2 cut(s) 674, 913
Cfr13I GGNCC 8 cut(s) 750, 822, 1556, 1914, 2616, 2783, 3158, 4225
ClaI ATCGAT 1 cut(s) 3508
Csp6I GTAC 5 cut(s) 233, 608, 2368, 2698, 3751
CviQI GTAC 5 cut(s) 233, 608, 2368, 2698, 3751
DraI TTTAAA 1 cut(s) 2463
EaeI YGGCCR 2 cut(s) 714, 3577
Eam1104I CTCTTC 6 cut(s) 1298, 1974, 2201, 2587, 2853, 3689
EarI CTCTTC 6 cut(s) 1298, 1974, 2201, 2587, 2853, 3689
Ecl136II GAGCTC 1 cut(s) 3917
Eco24I GRGCYC 2 cut(s) 1017, 3919
Eco31I GGTCTC 1 cut(s) 2928
Eco32I GATATC 1 cut(s) 1657
Eco47I GGWCC 7 cut(s) 750, 1556, 1914, 2616, 2783, 3158, 4225
Eco53kI GAGCTC 1 cut(s) 3917
Eco57I CTGAAG 7 cut(s) 1985, 1998, 2568, 2783, 2909, 3105, 3801
Eco81I CCTNAGG 2 cut(s) 219, 452
Eco88I CYCGRG 1 cut(s) 855
Eco91I GGTNACC 2 cut(s) 928, 2533
EcoICRI GAGCTC 1 cut(s) 3917
EcoO109I RGGNCCY 2 cut(s) 1556, 2616
EcoO65I GGTNACC 2 cut(s) 928, 2533
EcoRI GAATTC 3 cut(s) 570, 1221, 4150
EcoRII CCWGG 2 cut(s) 296, 2117
EcoRV GATATC 1 cut(s) 1657
EcoT22I ATGCAT 2 cut(s) 1818, 3865
EcoT38I GRGCYC 2 cut(s) 1017, 3919
FaqI GGGAC 4 cut(s) 1126, 1927, 3947, 4087
FauI CCCGC 2 cut(s) 347, 3621
FauNDI CATATG 1 cut(s) 3149
FbaI TGATCA 4 cut(s) 1021, 1549, 1752, 1864
FblI GTMKAC 1 cut(s) 4340
Fnu4HI GCNGC 8 cut(s) 419, 699, 1091, 1294, 1713, 3389, 4248, 4251
FriOI GRGCYC 2 cut(s) 1017, 3919
Fsp4HI GCNGC 8 cut(s) 419, 699, 1091, 1294, 1713, 3389, 4248, 4251
FspBI CTAG 6 cut(s) 230, 663, 2100, 3131, 3318, 3884
GlaI GCGC 2 cut(s) 673, 912
GluI GCNGC 8 cut(s) 419, 699, 1091, 1294, 1713, 3389, 4248, 4251
GsuI CTGGAG 1 cut(s) 2592
HaeII RGCGCY 1 cut(s) 914
HaeIII GGCC 5 cut(s) 716, 823, 890, 3579, 3677
HapII CCGG 2 cut(s) 384, 4270
HhaI GCGC 2 cut(s) 674, 913
Hin1I GRCGYC 1 cut(s) 3936
Hin6I GCGC 2 cut(s) 672, 911
HinP1I GCGC 2 cut(s) 672, 911
HincII GTYRAC 3 cut(s) 2182, 3334, 3659
HindII GTYRAC 3 cut(s) 2182, 3334, 3659
HindIII AAGCTT 5 cut(s) 1767, 2474, 3974, 4157, 4350
HpaII CCGG 2 cut(s) 384, 4270
HphI GGTGA 7 cut(s) 341, 922, 991, 1261, 1762, 2918, 3292
Hpy166II GTNNAC 7 cut(s) 852, 2182, 2786, 3234, 3334, 3659, 4341
Hpy8I GTNNAC 7 cut(s) 852, 2182, 2786, 3234, 3334, 3659, 4341
Hpy99I CGWCG 1 cut(s) 830
HpyCH4III ACNGT 8 cut(s) 1790, 2367, 2470, 2671, 2697, 3254, 3331, 3500
HpyCH4IV ACGT 3 cut(s) 1059, 3661, 3936
HpyF10VI GCNNNNNNNGC 6 cut(s) 75, 259, 998, 1457, 3170, 3884
HpySE526I ACGT 3 cut(s) 1059, 3661, 3936
Hsp92I GRCGYC 1 cut(s) 3936
HspAI GCGC 2 cut(s) 672, 911
Ksp22I TGATCA 4 cut(s) 1021, 1549, 1752, 1864
LguI GCTCTTC 2 cut(s) 1298, 3689
LmnI GCTCC 4 cut(s) 1301, 1637, 3169, 3914
Lsp1109I GCAGC 6 cut(s) 405, 685, 1102, 3400, 4234, 4237
MaeI CTAG 6 cut(s) 230, 663, 2100, 3131, 3318, 3884
MaeII ACGT 3 cut(s) 1059, 3661, 3936
MbiI CCGCTC 1 cut(s) 1296
MfeI CAATTG 5 cut(s) 459, 480, 1079, 1461, 1517
MflI RGATCY 6 cut(s) 181, 595, 1167, 1598, 2040, 3065
MhlI GDGCHC 4 cut(s) 1017, 1474, 3434, 3919
MlsI TGGCCA 2 cut(s) 716, 3579
MluNI TGGCCA 2 cut(s) 716, 3579
MlyI GAGTC 5 cut(s) 82, 2617, 3089, 3277, 3664
MmeI TCCRAC 1 cut(s) 2835
Mox20I TGGCCA 2 cut(s) 716, 3579
Mph1103I ATGCAT 2 cut(s) 1818, 3865
MroXI GAANNNNTTC 2 cut(s) 3509, 3968
MscI TGGCCA 2 cut(s) 716, 3579
MslI CAYNNNNRTG 5 cut(s) 50, 2034, 2169, 2729, 3450
Msp20I TGGCCA 2 cut(s) 716, 3579
MspA1I CMGCKG 1 cut(s) 602
MspCI CTTAAG 3 cut(s) 703, 1241, 2296
MspI CCGG 2 cut(s) 384, 4270
MspR9I CCNGG 2 cut(s) 298, 2119
MunI CAATTG 5 cut(s) 459, 480, 1079, 1461, 1517
Mva1269I GAATGC 2 cut(s) 2501, 3865
MvaI CCWGG 2 cut(s) 298, 2119
MwoI GCNNNNNNNGC 6 cut(s) 75, 259, 998, 1457, 3170, 3884
NcoI CCATGG 6 cut(s) 263, 343, 515, 891, 975, 3588
NdeI CATATG 1 cut(s) 3149
NheI GCTAGC 1 cut(s) 3883
NlaIV GGNNCC 8 cut(s) 597, 751, 1169, 1557, 1915, 2618, 2829, 3067
NmeAIII GCCGAG 1 cut(s) 4279
NmuCI GTSAC 6 cut(s) 4, 43, 928, 1209, 2671, 3280
NsiI ATGCAT 2 cut(s) 1818, 3865
NspI RCATGY 3 cut(s) 2374, 3455, 4328
NspV TTCGAA 3 cut(s) 2059, 2066, 3962
OliI CACNNNNGTG 1 cut(s) 50
PagI TCATGA 3 cut(s) 84, 3639, 3811
PciSI GCTCTTC 2 cut(s) 1298, 3689
PctI GAATGC 2 cut(s) 2501, 3865
PdmI GAANNNNTTC 2 cut(s) 3509, 3968
PflFI GACNNNGTC 1 cut(s) 91
PflMI CCANNNNNTGG 3 cut(s) 264, 2570, 2968
PkrI GCNGC 8 cut(s) 420, 700, 1092, 1295, 1714, 3390, 4249, 4252
PleI GAGTC 5 cut(s) 82, 2617, 3088, 3277, 3663
PpsI GAGTC 5 cut(s) 82, 2617, 3088, 3277, 3663
PpuMI RGGWCCY 2 cut(s) 1556, 2616
PsiI TTATAA 3 cut(s) 1175, 4182, 4311
Psp124BI GAGCTC 1 cut(s) 3919
Psp1406I AACGTT 1 cut(s) 1059
Psp5II RGGWCCY 2 cut(s) 1556, 2616
Psp6I CCWGG 2 cut(s) 296, 2117
PspEI GGTNACC 2 cut(s) 928, 2533
PspGI CCWGG 2 cut(s) 296, 2117
PspN4I GGNNCC 8 cut(s) 597, 751, 1169, 1557, 1915, 2618, 2829, 3067
PspPI GGNCC 8 cut(s) 750, 822, 1556, 1914, 2616, 2783, 3158, 4225
PspPPI RGGWCCY 2 cut(s) 1556, 2616
PstI CTGCAG 1 cut(s) 3407
PsuI RGATCY 6 cut(s) 181, 595, 1167, 1598, 2040, 3065
PsyI GACNNNGTC 1 cut(s) 91
PvuII CAGCTG 1 cut(s) 602
RsaI GTAC 5 cut(s) 234, 609, 2369, 2699, 3752
RsaNI GTAC 5 cut(s) 233, 608, 2368, 2698, 3751
RseI CAYNNNNRTG 5 cut(s) 50, 2034, 2169, 2729, 3450
SacI GAGCTC 1 cut(s) 3919
SapI GCTCTTC 2 cut(s) 1298, 3689
SatI GCNGC 8 cut(s) 419, 699, 1091, 1294, 1713, 3389, 4248, 4251
Sau96I GGNCC 8 cut(s) 750, 822, 1556, 1914, 2616, 2783, 3158, 4225
SchI GAGTC 5 cut(s) 82, 2617, 3089, 3277, 3664
ScrFI CCNGG 2 cut(s) 298, 2119
SduI GDGCHC 4 cut(s) 1017, 1474, 3434, 3919
SfcI CTRYAG 3 cut(s) 2693, 3403, 3739
SfuI TTCGAA 3 cut(s) 2059, 2066, 3962
SinI GGWCC 7 cut(s) 750, 1556, 1914, 2616, 2783, 3158, 4225
SmiMI CAYNNNNRTG 5 cut(s) 50, 2034, 2169, 2729, 3450
SsiI CCGC 6 cut(s) 340, 1294, 1713, 3386, 3628, 3748
SspI AATATT 4 cut(s) 870, 2193, 2314, 2957
SspMI CTAG 6 cut(s) 230, 663, 2100, 3131, 3318, 3884
SstI GAGCTC 1 cut(s) 3919
StyD4I CCNGG 2 cut(s) 296, 2117
TaaI ACNGT 8 cut(s) 1790, 2367, 2470, 2671, 2697, 3254, 3331, 3500
TaiI ACGT 3 cut(s) 1062, 3664, 3939
TatI WGTACW 1 cut(s) 2367
TauI GCSGC 2 cut(s) 1296, 1715
TseFI GTSAC 6 cut(s) 4, 43, 928, 1209, 2671, 3280
TseI GCWGC 6 cut(s) 418, 698, 1090, 3388, 4247, 4250
Tsp45I GTSAC 6 cut(s) 4, 43, 928, 1209, 2671, 3280
TspGWI ACGGA 2 cut(s) 1130, 4091
Tth111I GACNNNGTC 1 cut(s) 91
Van91I CCANNNNNTGG 3 cut(s) 264, 2570, 2968
Vha464I CTTAAG 3 cut(s) 703, 1241, 2296
VpaK11BI GGWCC 7 cut(s) 750, 1556, 1914, 2616, 2783, 3158, 4225
XapI RAATTY 9 cut(s) 570, 1221, 1744, 2103, 2234, 2269, 2403, 2992, 4150
XbaI TCTAGA 2 cut(s) 2099, 3130
XceI RCATGY 3 cut(s) 2374, 3455, 4328
XcmI CCANNNNNNNNNTGG 3 cut(s) 1279, 1780, 2994
XmiI GTMKAC 1 cut(s) 4340
XmnI GAANNNNTTC 2 cut(s) 3509, 3968
XspI CTAG 6 cut(s) 230, 663, 2100, 3131, 3318, 3884
ZraI GACGTC 1 cut(s) 3937
Zsp2I ATGCAT 2 cut(s) 1818, 3865
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.