Rw0G004790

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00183
OG0000085
Physical Location & Seq
Forward (+)
7104 .. 21316
14213 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G004790.1

Sequence Viewer

Length: 6807 bp
ATGGAGTTCTGCCAGTATGTCTTTGAGGCTATGAAGATGATTATTGTGATTAGTGCGGTAATGGTGCAATGGTTCCGTGTCATAGCCTGTCTTAAGTGGATATGGAAGAATTGGTTCCTTATTGTGGTGATTGTGACATGGAACGTAATAGCCAGTCTTGGGTGGATATGGAAGAGTAGGTTCAGTTACATGGTTGAAACCCCAGAGCCAATATCAGAGGATAATCAAGAACCAGTTGACGTCGATGTCTCTGCATCTCTTCCTTCATCATCAGCTGAATTAACAGCTCCTACGTGGAAGCATGATGTGTTTTTGAGCTTCAGGGGTGTAGACACGCGTAAGAATATTACATTCTTTTTATACAATCGGCTGCAAGAAAGGGAAATCAAAACATTCATGGATGAACGAGACATTGAAGTAGGGGATGTTATTTCTCCCACTCTCCTAACGGCAATTGAAGAATCAAGGTTGGCGATTATTGTTCTCTCTCAAAACTATGCCTCTTCCTCTTGGTGTTTGGAGGAACTAGGACACATTTGTAAAAGCATGAAAGGAGACGAGTCAGACGACAATAGAATTCTGCCACTTTTTTATAATGTGCATCCTAGTGATGTACGACATCAGACGAGCAGGTTCGGAGATGCTTTCACTAAGCTTAAGAAATCTGGGCGACACGAATCAGCGAAGGTGGAGCAGTGGATAGATACTTTAACAAAAGTTGCACAAATATCCGGGTGGCATTCACTGGAATTTAAAAATGACGGAGAACTTTTGAATAACATTGTGGAAGCTGTGTGCAAGAAATTACTACCGACTGAATCTGTGTTAAAAATACCCCTCGGAGATTTTGAAGAATTTGCAGCAACAAAAGAAGCCATTCATGAGGTCATGAAGGCGCTAAAAGATGGTGAGGCCACTACCATTGGAGTCTACGGCATGGGGGGCGTTGGAAAGACAACAATGGTGAAACATGTCGGTGTACAAGCCCAAGAAGAAAAGCTTTTTAATAAAGTGATTATGGCTGTCGTATCCCAAAAACCCGACTTGAAGGAAATTCAACAGGAATTTGCAGAAACGCTGGGCTTTGAACTCAAGGAGAAGACGGAAATGGTAAGAGCCCGTAAATTGAAGATGAAAATAATGAGCGAAACCAGGATCCTCATAATCTTGGATGACATTTGGAAGAGAATAGACTTTTCAAGAATTGGAATTCCGAGCGACAACGAACTTCAAAAATGCAATTCCAAAGTCCTATTGACCACCAGAAGTTTGAAACTTTGTAGGATGATGGGGACCCATGCAAAAATTCCCCTCAATATCTTATCAGAAGAAGATTCTTGGCGCTTATTTGTGAGGGAAGCGGGGATGTCTTCTGACAAATCTTCCAATTTCTCTGATGTAGCGAGCAAGGTGGCAAGAGAATGCGCCGGTCTACCAGTTGCTTTGATAGCAGTTGCGAGGGCCCTTCGAGATGAAGATTTGGACAGATGGGAAGAAGCTGCACGACAACTAAAAGCGTCTCAACCTCCCATCCGTGAAGATGAGGGAGATGTGTTTGCATGCATAAAGTTGAGCTATGATCACTTGAAATCTGATGATTCCAAATCATGCTTCTTGCTTTGCTGCTTGTTCCCAGAAGATTATAATATCCCAATTGAATACTTGGTCATGTATGGAATTGGGAAAGGAATGTTTCAAGATTTAACCATGCAACGAGCCCGAGACTCAACATATTCAGTGGTGCAGGACCTTAAAGATTCTAGCTTGCTTTTAGGCGGTAGAGATGACGGATGTGTAAGGATGCATGATGTCATTCGGGATACGGCAATACGAATTTCCTCATCTGAAGATGGCCAGCAGTCCTTGGTGAAAGTTGGCTGTGAATTGACGAATTGGCCAAAGATTGATGCACATAAAGGCTACTCTGCAATCTCACTAATGAAGAATAAAATTTGCAAGCTACCAAAAAGGTTGGTATGTCCAAATCTCCAGATTTTATTACTACGACAGAATGCTTCTTTATATCATATCCCAGAATCTTTTTTCCAAAGTCAGAATGAATTAAGAGTCTTGGATCTTAGCGACACTCGTATTTCATTACTACAATCAATCGGTTTCCTAACCAACCTTCAAGCTTTGTATTTAGATTATTGCAGAAACATAATTGACACTTCTGTAATCGGAAAACTTCACAAGCTTGAGATTCTTAGTATGAGAGGAACTGCTCTGAAAGAATTGTCAAGAGAAATAGAACAACTGACCAATCTAAGGATGCTCGATATAAGTGCTGCAGCTTTATCCTTGAGAGGAAGTATTTGCAAAATTCCATCTAAAGTGATATCAAAGTTGCATAAATTAGAAGAGCTGTACATGCAATATGCATTTTGGGACTGGGGGAGGAAAATTGATGGAAAAGGAGAAGAAACTAATATTGGCTTTGATGAATTAGCTGGTTTATCATATTTAAGAATTTTGAAAGTTTGCATATTAGATGCAAATTGCATCCCTAAAAGTGTTGAGGTCGAACCGAATTGGGATTACTTTGATATTAGTATTTGTAGCGGCAAGAGTGGACAGGGAATCTATAACATCCTTCGATCTGGTAATAATTATAGATCCTTGAGTCTTGATAGTACTGACACAACCATGGGAACCTTTCCGGACTGGTTTGTCAACGCTGTGGTAAAGAATACTGAGAGGCTGCGTTATGAAGAATGCAAATGCTTAAGTAACATTCTTGTGGAATATGACCGTGGGAAGTTACATGGACTGAAAGTTCTCTCTGTAAAAGGTCGCTGTGAGAACTTGAAAGAATTGATGAATGCAATAACATGTGTTCCAGATCCAGATATGCCTGTGTTTGAGAAGTTGGAAGAGTTGTATCTGGAATACCTGGATGACCTGAAGCAGTTATGTGTTGGTGAGTTACCACCTCGATCTCTCTGCAATCTGAAGTTATTGAAAGTCGCTTGCTGTCATAACTTGGGGAATTTGCTGTTGCCATCAAAATTGTTGCAGAAACTACCAAATTTGGAAATACTAAACTGTGAGGAAAGTAAAGTGGATCATGTGTTTGAATGTATAGGATTTGAGCCAGATCAAACAAATCTGAGAGAGATGGAGTTGCGAGATCTAGACGTAATAAGAAGCATATGTAATGGTCCTGCTCCACGCAGAATGTTCCAGGCTCTAAAGCAATTGGTAATTCACAATTGCAACTTCCAAGGAAGTCTCTTCACATTTGATGTAGCTCAGTGTCTTTTTCAATTGGAAAGCCTTGTTGTATCCGAATGCCTTGTATTGGAAAGAGTAATCGAAGCAAGGAGGGAAACATTGCACAACAAGACCGTTCTTCCAAAATTGAAGAACTTATGTTTGCAATATCTTCCAATGTTGTACAAGGGAAGTGCTACTGTTGATTTTGAGTGTCCTTCATTGGAAAAATTGTGTCTGCTTGGCTGCCCCCACTTGTCATTTCAATCCCCTGCTTCTGACTACTTCCACAGCAGGAACCCAGTCAATGAAGATTGGAAACCGGTTGAAGTCGATGTCTCTGCACCTCTTCCTTCAACATCAGCTGAATCATCAGCTCCGAGGTGGAAGCATGATGTGTTTTTGAGTTTCAGGGGTGGAGACACTCGCAAAGGTATTGCATTCGAATTATACGATCGACTGCAAAACAGGAGAGGAATCAAAACATTCATGGATGACCAAGACCTTCAAGTAGGGGATGTTATTTCTCCCACTCTCCTAACGGCGATTAAAGAATCAAGGCTTGCAATTATTGTTCTCTCTCAAAACTATGCCTCTTCCACTTGGTGTTTGGAGGAACTTAGAAACATTTGTGAATGCATGGAAGAAGACAACAACAGAATTCTGCCACTTTTTTATTATGTGGATCCTACTGATGTACGATATCAGAAGAGCAGTTTCGGAGATGCTTTCACTAAGCATGAAAACTCTGGGCGACACAGATCAGAGAAGGTGCAGCAGTGGAGAGATGCTTTAAAAAAAGTGGCAAATTTCTCTGGGTGGCATACACTGAATTATAAAACTGAAAGAGAACTTTTGGATGCCATTCAGGAATCTGTGTGCAATAAATTACGACCTACTGAACTTGAGTTTAAAATGTCCATCGGAGGTTTTGAAGCATTTAAAGCAACAAAACAAGCCATTGATGAGGTCATGAATGCGCTAAAAGATGATGAGGCCACTTGCATTGGAGTCTACGGAATGGGGGGCGTCGGAAAAACGACAATGGTGAAATATGTTGGAGCACTAGCCCAAGAAAGTAGGCTTTTTAATCAAGTGATTATGGCTGTCGTATCCCAAAACCCCGACCTGATGAAAATTCAAGAGACATTTGCAGAACTGCTGGGCTTTAAATTGGAGGAGAAGACAGAAATTGGAAGAGCCATTAAATTGAAGGGGAAGATAATGAGAGGAACCGGGATCCTCATAATCTTGGATGACATTTGGAAGAGAATAGACTTATCAGACATTGGAATTCCGAGCCACAACGAACTTCAAAAATGCAATTCCAAAGTCCTACTGACCACCAGAAGATTGAGTGTTTGTGAGATCATGATGAGCCATGCAAAAATCCAGCTCAATATCTTATCAAAAGAAGATTCTTGGAGCTTATTTGTGAAGGAAGCAGGGAAGTCTTTTGACAAATCTTCCAATTTCTATGATGTAGCACGTGAGGTGGCAAGAGAATGCGCCGGTCTACCAGTTGCTTTGATAGCAGTTGCGAGGGCCCTTCGAGGTGAAGGCTTGGACGGATGGAAAGAAGCTGCTCGACGACTAAAAGTGTCTCAACCTCCCATCCCGGAAGATGAGGAAGATGTGTTCAAATGCATAAAGTTAAGCTATGATTACTTGAAATCTGATGATTCCAAATCATGCTTCTTGCTTTGCTGCCTGTTCCCAGAAGATTATGATATCCCAATTGAATGCTTGTTCAAGTATGGAATTGGGAAAGGAATGTTTCAAGATTCCTACATGCTAGAAGCCCGAGGCAGAACAGATTCATTGGTGAATGCCCTTAAAGATTCTAGCTTGCTTTTGGACGCTAGAGATGACAGATTTGTAAGGATGCATGATGTCATTCGGGATATGGCAATACGAATTTCCTTATCTGAAGGCCAGCCATTCCTTGTGAAAGCTGGCTGTGAATTGAAGAATTGGCCAAAGATTGATGCACATAAAGGCTGCTCTGCAATCTCACTAATGAGGAACATAATTTGCAAGCTACCCGAAGAGTTGGTATGTCCAAATCTCCAGATTTTATTACTACAACAGAATGATTCTTTAAATGAGATCCCAAAATCTTTTTTCCGAAGTCAGAATGAATTAAGAGTCTTAGATCTTGGCGGCACTGGTATTTCATTACTACCCCATTCAATCAGTTTCCTAACCAACCTTCAAGCTTTGTATTTAGATTATTGCCGCAACATAATTGACACTTCTGTAATGGGAAAACTTCACAAGCTTGAGATTCTTAGTATGAGAGGAACTGATCTGGAAGAATTGTCAAGAGAAATAGGACAATTGACCAATCTAAGGATGCTTGATGTCAGTGGTGCTGGATTTATTTGCACAATTCCATCTAAAGTGATATCAAAGTTGCATAAATTAGAAGAGCTGTACATGCAATGTGCATTTTGGGACTGGGGGAGTAAAATTGATGGAGAAGGAGAAGAAACCAATATTGGCTTTGATGAATTAGCTGGTTTATCATATTTACGCATTTTGGAAGTTTGCATATCAGATGAAAATTGCATCCCTAGAAGTGTTGAGGTCGAACCGAATTGGGATTACTTCGATATTCGCATTTGCAGCGACGGTGATGGAAAGATTGAAATTATGAGCTATCTATCTGGTGATTCTGGTCGGAAGTGTAGATCCTTGGGTCTTCATAGTACTGACACAACCATAGGAACCTTTCCGGACTGGTTTGTCAACGCTGTGGTAAAGAAAACTGAGAGGCTGAGTTATGAAGAATGCACAGGGTTGAGTAACATTCTTGTGGAATATGACCGTGGGAGGTTACATGGACTGAAAGTTCTCTATGTAAGATATTGCTGTGAGAACTTGAAAGAATTGATGAATGCAATAACATTTGTATCTGAAAATCTGGATGACCTGAAGCAGTTATGTGTTGGTGAGTTACCACCTGGATCTCTCTGCAGTCTGAAATTATTGATCGTCTGTGAGTGTCGTAACTTGGGGAATGTGCTGTTGCCATCAAAATTGTTGCAGAAACTACCAAATCTGGAAAAACTAATATGTGGGGTAAGTAGAGTGGAATATGTGTTTGGATGTGAAGGATTTGAGCCGGATCAAACAAATCTGAGAGAGATGAAGTTGTTCGATCTAGACGCAGTGAGAAGCATATGTAATGGTCTTGCTCCACGTGGAATGTTCCAGGCTCTAAAGGAATTGGCCATTGAGAGGTGCAACATCCAGGGAAGTCTCTTCACAATTGATGAAGCTCAGTGTCTTTTTCAATTGGAAAGCCTTGTTGTATCCAGTTGCCCTGTCTTGGAAAGAGTAATCGAAGCAAGGAGGGAAACATTGAACAGCAAGAAGACCGTTCTTCCAAAAATGAAGAACTTATGTTTGCGATATCTTCCAATGTTGTACGAGGGAAGTGCTACTGTTGATTTTGAGTGTCCTTCATTGGAAAACTTGCTTCTGAGGGACTGCCCCCACTTGTCATTTCAATCCTCTGCTTCTGACTACTTCCACAGCAGGAACCAAGTCAATGAAGATCTGAGTCTGTCATATATTTATATGAATTGA

Protein Analysis

2268

Amino Acids

257.32

Weight (kDa)

5.63

Isoelectric Point (pI)

46.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 100 - 272 2.3e-46 TIR domain
TIR_2 PF13676 103 - 203 1.1e-13 TIR domain
NB-ARC PF00931 291 - 458 7.4e-31 NB-ARC domain
LRR_8 PF13855 661 - 719 2.4e-07 Leucine rich repeat
LRR_14 PF23598 674 - 798 4.6e-11 Leucine-rich repeat region
LRR_RPS2 PF23247 957 - 1098 1.1e-18 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1037 - 1157 6.7e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
TIR PF01582 1201 - 1372 4.8e-50 TIR domain
TIR_2 PF13676 1204 - 1301 2.3e-15 TIR domain
NB-ARC PF00931 1389 - 1555 9.3e-30 NB-ARC domain
LRR_8 PF13855 1759 - 1818 1.2e-07 Leucine rich repeat
LRR_14 PF23598 1822 - 1886 5.5e-08 Leucine-rich repeat region
LRR_RPS2 PF23247 2048 - 2180 1.1e-17 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 2120 - 2240 8.2e-09 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 594, 1644, 4050
AasI GACNNNNNNGTC 3 cut(s) 245, 2669, 5960
AatII GACGTC 1 cut(s) 243
Acc36I ACCTGC 1 cut(s) 621
AccI GTMKAC 5 cut(s) 330, 930, 1432, 4227, 4729
AccII CGCG 1 cut(s) 337
AccIII TCCGGA 2 cut(s) 2659, 5950
AciI CCGC 6 cut(s) 56, 1361, 1776, 2562, 5376, 5452
AcoI YGGCCR 4 cut(s) 1852, 1895, 5189, 6447
AcuI CTGAAG 6 cut(s) 304, 1866, 2924, 2972, 5163, 6170
AcvI CACGTG 2 cut(s) 4703, 6419
AcyI GRCGYC 2 cut(s) 240, 4242
AdeI CACNNNGTG 1 cut(s) 3819
AfaI GTAC 9 cut(s) 615, 981, 2369, 2635, 3398, 3912, 5651, 5926, 6647
AfiI CCNNNNNNNGG 7 cut(s) 124, 159, 2268, 3301, 5565, 6456, 6547
AflII CTTAAG 3 cut(s) 92, 656, 2725
AflIII ACRYGT 3 cut(s) 335, 970, 2831
AgeI ACCGGT 1 cut(s) 3535
AjnI CCWGG 6 cut(s) 1151, 2892, 3183, 6178, 6429, 6468
AloI GAACNNNNNNTCC 6 cut(s) 2760, 2792, 4835, 4867, 6051, 6083
Alw21I GWGCWC 1 cut(s) 4279
Ama87I CYCGRG 2 cut(s) 1719, 5016
Aor13HI TCCGGA 2 cut(s) 2659, 5950
AoxI GGCC 9 cut(s) 912, 1461, 1852, 1895, 4209, 4758, 5146, 5189, 6447
ApaI GGGCCC 2 cut(s) 1465, 4762
AsiGI ACCGGT 1 cut(s) 3535
Asp700I GAANNNNTTC 4 cut(s) 113, 876, 5029, 6371
AspLEI GCGC 5 cut(s) 898, 1344, 1427, 4195, 4724
AspS9I GGNCC 7 cut(s) 1293, 1461, 1462, 1747, 3161, 4758, 4759
AsuC2I CCSGG 3 cut(s) 733, 4450, 4832
AsuII TTCGAA 1 cut(s) 3657
AvaI CYCGRG 2 cut(s) 1719, 5016
AvaII GGWCC 3 cut(s) 1293, 1747, 3161
BaeGI GKGCMC 2 cut(s) 1465, 4762
BaeI ACNNNNGTAYC 2 cut(s) 6111, 6144
BalI TGGCCA 4 cut(s) 1854, 1897, 5191, 6449
BamHI GGATCC 3 cut(s) 1155, 3898, 4452
BanII GRGCYC 4 cut(s) 1120, 1465, 1720, 4762
BbrPI CACGTG 2 cut(s) 4703, 6419
BbsI GAAGAC 6 cut(s) 1106, 1362, 3867, 4403, 5909, 6599
Bbv12I GWGCWC 1 cut(s) 4279
BceAI ACGGC 4 cut(s) 465, 949, 1839, 3771
BciT130I CCWGG 6 cut(s) 1153, 2894, 3185, 6180, 6431, 6470
BciVI GTATCC 5 cut(s) 1039, 1813, 3295, 4336, 6541
BclI TGATCA 1 cut(s) 1579
BcnI CCSGG 3 cut(s) 733, 4450, 4832
BfmI CTRYAG 2 cut(s) 2289, 6190
BfoI RGCGCY 2 cut(s) 899, 1345
BfrI CTTAAG 3 cut(s) 92, 656, 2725
BfuAI ACCTGC 1 cut(s) 621
BfuI GTATCC 5 cut(s) 1039, 1813, 3295, 4336, 6541
BglII AGATCT 3 cut(s) 3130, 5368, 6775
BmcAI AGTACT 2 cut(s) 2635, 5926
Bme1390I CCNGG 9 cut(s) 733, 1153, 2894, 3185, 4450, 4832, 6180, 6431, 6470
Bme18I GGWCC 3 cut(s) 1293, 1747, 3161
BmeT110I CYCGRG 2 cut(s) 1719, 5016
BmgT120I GGNCC 7 cut(s) 1293, 1461, 1462, 1747, 3161, 4758, 4759
BmrFI CCNGG 9 cut(s) 733, 1153, 2894, 3185, 4450, 4832, 6180, 6431, 6470
BmrI ACTGGG 3 cut(s) 2401, 3509, 5683
BmuI ACTGGG 3 cut(s) 2401, 3509, 5683
BpiI GAAGAC 6 cut(s) 1106, 1362, 3867, 4403, 5909, 6599
BpmI CTGGAG 2 cut(s) 1973, 5267
Bpu14I TTCGAA 1 cut(s) 3657
BpuEI CTTGAG 6 cut(s) 1076, 2219, 2323, 2641, 4139, 5516
BpuMI CCSGG 3 cut(s) 733, 4450, 4832
BsaAI YACGTR 3 cut(s) 294, 4703, 6419
BsaHI GRCGYC 2 cut(s) 240, 4242
BsaWI WCCGGW 3 cut(s) 2659, 3535, 5950
Bsc4I CCNNNNNNNGG 7 cut(s) 124, 159, 2268, 3301, 5565, 6456, 6547
Bse118I RCCGGY 3 cut(s) 1427, 3535, 4724
Bse3DI GCAATG 3 cut(s) 74, 3332, 5663
BseAI TCCGGA 2 cut(s) 2659, 5950
BseBI CCWGG 6 cut(s) 1153, 2894, 3185, 6180, 6431, 6470
BseLI CCNNNNNNNGG 7 cut(s) 124, 159, 2268, 3301, 5565, 6456, 6547
BseMI GCAATG 3 cut(s) 74, 3332, 5663
BseMII CTCAG 9 cut(s) 2685, 3101, 3266, 5976, 5984, 6347, 6512, 6692, 6770
BseRI GAGGAG 1 cut(s) 4406
BseSI GKGCMC 2 cut(s) 1465, 4762
BseYI CCCAGC 2 cut(s) 1078, 4375
BsgI GTGCAG 4 cut(s) 1485, 1763, 3540, 4007
Bsh1236I CGCG 1 cut(s) 337
Bsh1285I CGRYCG 1 cut(s) 3670
BshFI GGCC 9 cut(s) 914, 1463, 1854, 1897, 4211, 4760, 5148, 5191, 6449
BshTI ACCGGT 1 cut(s) 3535
BsiEI CGRYCG 1 cut(s) 3670
BsiHKAI GWGCWC 1 cut(s) 4279
BsiHKCI CYCGRG 2 cut(s) 1719, 5016
BsiSI CCGG 9 cut(s) 732, 1428, 2660, 3536, 4449, 4725, 4832, 5951, 6341
BslFI GGGAC 4 cut(s) 1306, 2402, 5684, 6719
BslI CCNNNNNNNGG 7 cut(s) 124, 159, 2268, 3301, 5565, 6456, 6547
BsmBI CGTCTC 2 cut(s) 549, 1524
BsmFI GGGAC 4 cut(s) 1306, 2402, 5684, 6719
BsnI GGCC 9 cut(s) 914, 1463, 1854, 1897, 4211, 4760, 5148, 5191, 6449
BsoBI CYCGRG 2 cut(s) 1719, 5016
Bsp119I TTCGAA 1 cut(s) 3657
Bsp120I GGGCCC 2 cut(s) 1461, 4758
Bsp1286I GDGCHC 5 cut(s) 1120, 1465, 1720, 4279, 4762
Bsp13I TCCGGA 2 cut(s) 2659, 5950
Bsp1407I TGTACA 4 cut(s) 979, 2367, 3396, 5649
Bsp19I CCATGG 1 cut(s) 2646
BspACI CCGC 6 cut(s) 56, 1361, 1776, 2562, 5376, 5452
BspANI GGCC 9 cut(s) 914, 1463, 1854, 1897, 4211, 4760, 5148, 5191, 6449
BspCNI CTCAG 9 cut(s) 2686, 3102, 3265, 5977, 5985, 6348, 6511, 6693, 6771
BspEI TCCGGA 2 cut(s) 2659, 5950
BspFNI CGCG 1 cut(s) 337
BspHI TCATGA 4 cut(s) 880, 888, 4185, 4584
BspMAI CTGCAG 2 cut(s) 2293, 6194
BspMI ACCTGC 1 cut(s) 621
BspQI GCTCTTC 4 cut(s) 2355, 3917, 4405, 5637
BspT104I TTCGAA 1 cut(s) 3657
BspTI CTTAAG 3 cut(s) 92, 656, 2725
BsrDI GCAATG 3 cut(s) 74, 3332, 5663
BsrFI RCCGGY 3 cut(s) 1427, 3535, 4724
BsrGI TGTACA 4 cut(s) 979, 2367, 3396, 5649
BssAI RCCGGY 3 cut(s) 1427, 3535, 4724
BssNI GRCGYC 2 cut(s) 240, 4242
BssT1I CCWWGG 4 cut(s) 1863, 2646, 3223, 5910
Bst2UI CCWGG 6 cut(s) 1153, 2894, 3185, 6180, 6431, 6470
Bst4CI ACNGT 8 cut(s) 2753, 3047, 3349, 3415, 5849, 6044, 6598, 6664
BstACI GRCGYC 2 cut(s) 240, 4242
BstAFI CTTAAG 3 cut(s) 92, 656, 2725
BstAUI TGTACA 4 cut(s) 979, 2367, 3396, 5649
BstBAI YACGTR 3 cut(s) 294, 4703, 6419
BstBI TTCGAA 1 cut(s) 3657
BstDSI CCRYGG 3 cut(s) 2646, 2752, 6043
BstFNI CGCG 1 cut(s) 337
BstH2I RGCGCY 2 cut(s) 899, 1345
BstHHI GCGC 5 cut(s) 898, 1344, 1427, 4195, 4724
BstMCI CGRYCG 1 cut(s) 3670
BstMWI GCNNNNNNNGC 7 cut(s) 942, 1448, 2371, 4157, 4745, 5653, 5841
BstNI CCWGG 6 cut(s) 1153, 2894, 3185, 6180, 6431, 6470
BstNSI RCATGY 6 cut(s) 974, 1563, 2374, 2835, 5008, 5656
BstSCI CCNGG 9 cut(s) 731, 1151, 2892, 3183, 4448, 4830, 6178, 6429, 6468
BstSFI CTRYAG 2 cut(s) 2289, 6190
BstSLI GKGCMC 2 cut(s) 1465, 4762
BstUI CGCG 1 cut(s) 337
BstV2I GAAGAC 6 cut(s) 1106, 1362, 3867, 4403, 5909, 6599
BsuI GTATCC 5 cut(s) 1039, 1813, 3295, 4336, 6541
BsuRI GGCC 9 cut(s) 914, 1463, 1854, 1897, 4211, 4760, 5148, 5191, 6449
BtgI CCRYGG 3 cut(s) 2646, 2752, 6043
BtsI GCAGTG 3 cut(s) 701, 3998, 6393
BveI ACCTGC 1 cut(s) 621
CciI TCATGA 4 cut(s) 880, 888, 4185, 4584
CfoI GCGC 5 cut(s) 898, 1344, 1427, 4195, 4724
Cfr10I RCCGGY 3 cut(s) 1427, 3535, 4724
Cfr13I GGNCC 7 cut(s) 1293, 1461, 1462, 1747, 3161, 4758, 4759
CseI GACGC 4 cut(s) 1506, 4231, 5081, 6392
Csp6I GTAC 9 cut(s) 614, 980, 2368, 2634, 3397, 3911, 5650, 5925, 6646
CspAI ACCGGT 1 cut(s) 3535
CspCI CAANNNNNGTGG 4 cut(s) 904, 939, 4201, 4236
CviQI GTAC 9 cut(s) 614, 980, 2368, 2634, 3397, 3911, 5650, 5925, 6646
DraI TTTAAA 6 cut(s) 754, 4008, 4126, 4156, 4384, 5316
DraIII CACNNNGTG 1 cut(s) 3819
DrdI GACNNNNNNGTC 3 cut(s) 245, 2669, 5960
DseDI GACNNNNNNGTC 3 cut(s) 245, 2669, 5960
EaeI YGGCCR 4 cut(s) 1852, 1895, 5189, 6447
Eco130I CCWWGG 4 cut(s) 1863, 2646, 3223, 5910
Eco24I GRGCYC 4 cut(s) 1120, 1465, 1720, 4762
Eco32I GATATC 5 cut(s) 2340, 3917, 4945, 5622, 6632
Eco47I GGWCC 3 cut(s) 1293, 1747, 3161
Eco57I CTGAAG 6 cut(s) 304, 1866, 2924, 2972, 5163, 6170
Eco72I CACGTG 2 cut(s) 4703, 6419
Eco88I CYCGRG 2 cut(s) 1719, 5016
EcoO109I RGGNCCY 6 cut(s) 1293, 1461, 1462, 1747, 4758, 4759
EcoRI GAATTC 4 cut(s) 576, 1209, 3873, 4506
EcoRII CCWGG 6 cut(s) 1151, 2892, 3183, 6178, 6429, 6468
EcoRV GATATC 5 cut(s) 2340, 3917, 4945, 5622, 6632
EcoT14I CCWWGG 4 cut(s) 1863, 2646, 3223, 5910
EcoT22I ATGCAT 6 cut(s) 1565, 1806, 2383, 3854, 4862, 5103
EcoT38I GRGCYC 4 cut(s) 1120, 1465, 1720, 4762
ErhI CCWWGG 4 cut(s) 1863, 2646, 3223, 5910
Esp3I CGTCTC 2 cut(s) 549, 1524
FalI AAGNNNNNCTT 2 cut(s) 984, 1016
FaqI GGGAC 4 cut(s) 1306, 2402, 5684, 6719
FauI CCCGC 1 cut(s) 1354
FauNDI CATATG 2 cut(s) 3152, 6398
FbaI TGATCA 1 cut(s) 1579
FblI GTMKAC 5 cut(s) 330, 930, 1432, 4227, 4729
FriOI GRGCYC 4 cut(s) 1120, 1465, 1720, 4762
GlaI GCGC 5 cut(s) 897, 1343, 1426, 4194, 4723
GsaI CCCAGC 2 cut(s) 1082, 4379
GsuI CTGGAG 2 cut(s) 1973, 5267
HaeII RGCGCY 2 cut(s) 899, 1345
HaeIII GGCC 9 cut(s) 914, 1463, 1854, 1897, 4211, 4760, 5148, 5191, 6449
HapII CCGG 9 cut(s) 732, 1428, 2660, 3536, 4449, 4725, 4832, 5951, 6341
HgaI GACGC 4 cut(s) 1506, 4231, 5081, 6392
HhaI GCGC 5 cut(s) 898, 1344, 1427, 4195, 4724
Hin1I GRCGYC 2 cut(s) 240, 4242
Hin6I GCGC 5 cut(s) 896, 1342, 1425, 4193, 4722
HinP1I GCGC 5 cut(s) 896, 1342, 1425, 4193, 4722
HincII GTYRAC 3 cut(s) 238, 2674, 5965
HindII GTYRAC 3 cut(s) 238, 2674, 5965
HindIII AAGCTT 6 cut(s) 653, 998, 2133, 2195, 5430, 5492
HpaII CCGG 9 cut(s) 732, 1428, 2660, 3536, 4449, 4725, 4832, 5951, 6341
Hpy99I CGWCG 4 cut(s) 245, 4247, 4806, 5849
HpyCH4III ACNGT 8 cut(s) 2753, 3047, 3349, 3415, 5849, 6044, 6598, 6664
HpyCH4IV ACGT 6 cut(s) 144, 240, 293, 3138, 4702, 6418
HpyF10VI GCNNNNNNNGC 7 cut(s) 942, 1448, 2371, 4157, 4745, 5653, 5841
HpySE526I ACGT 6 cut(s) 144, 240, 293, 3138, 4702, 6418
Hsp92I GRCGYC 2 cut(s) 240, 4242
HspAI GCGC 5 cut(s) 896, 1342, 1425, 4193, 4722
KflI GGGWCCC 1 cut(s) 1293
Kpn2I TCCGGA 2 cut(s) 2659, 5950
Ksp22I TGATCA 1 cut(s) 1579
LguI GCTCTTC 4 cut(s) 2355, 3917, 4405, 5637
LmnI GCTCC 7 cut(s) 292, 691, 3172, 3595, 4274, 4638, 6418
MaeII ACGT 6 cut(s) 144, 240, 293, 3138, 4702, 6418
MaeIII GTNAC 9 cut(s) 133, 185, 2729, 2760, 2925, 6020, 6051, 6171, 6224
MfeI CAATTG 9 cut(s) 453, 1653, 3197, 3211, 3266, 4950, 5552, 6486, 6512
MhlI GDGCHC 5 cut(s) 1120, 1465, 1720, 4279, 4762
MlsI TGGCCA 4 cut(s) 1854, 1897, 5191, 6449
MluI ACGCGT 1 cut(s) 335
MluNI TGGCCA 4 cut(s) 1854, 1897, 5191, 6449
MlyI GAGTC 8 cut(s) 569, 936, 1718, 2076, 2632, 4233, 5370, 6790
MmeI TCCRAC 5 cut(s) 928, 2850, 4225, 4252, 5876
Mox20I TGGCCA 4 cut(s) 1854, 1897, 5191, 6449
Mph1103I ATGCAT 6 cut(s) 1565, 1806, 2383, 3854, 4862, 5103
MroI TCCGGA 2 cut(s) 2659, 5950
MroXI GAANNNNTTC 4 cut(s) 113, 876, 5029, 6371
MscI TGGCCA 4 cut(s) 1854, 1897, 5191, 6449
MslI CAYNNNNRTG 5 cut(s) 606, 975, 2645, 2738, 6029
Msp20I TGGCCA 4 cut(s) 1854, 1897, 5191, 6449
MspA1I CMGCKG 2 cut(s) 275, 3578
MspCI CTTAAG 3 cut(s) 92, 656, 2725
MspI CCGG 9 cut(s) 732, 1428, 2660, 3536, 4449, 4725, 4832, 5951, 6341
MspR9I CCNGG 9 cut(s) 733, 1153, 2894, 3185, 4450, 4832, 6180, 6431, 6470
MunI CAATTG 9 cut(s) 453, 1653, 3197, 3211, 3266, 4950, 5552, 6486, 6512
MvaI CCWGG 6 cut(s) 1153, 2894, 3185, 6180, 6431, 6470
MvnI CGCG 1 cut(s) 337
MwoI GCNNNNNNNGC 7 cut(s) 942, 1448, 2371, 4157, 4745, 5653, 5841
NciI CCSGG 3 cut(s) 733, 4450, 4832
NcoI CCATGG 1 cut(s) 2646
NdeI CATATG 2 cut(s) 3152, 6398
NmuCI GTSAC 1 cut(s) 133
NsiI ATGCAT 6 cut(s) 1565, 1806, 2383, 3854, 4862, 5103
NspI RCATGY 6 cut(s) 974, 1563, 2374, 2835, 5008, 5656
NspV TTCGAA 1 cut(s) 3657
PaeI GCATGC 1 cut(s) 1563
PagI TCATGA 4 cut(s) 880, 888, 4185, 4584
PciI ACATGT 2 cut(s) 970, 2831
PciSI GCTCTTC 4 cut(s) 2355, 3917, 4405, 5637
PcsI WCGNNNNNNNCGW 2 cut(s) 564, 3663
PdmI GAANNNNTTC 4 cut(s) 113, 876, 5029, 6371
PfoI TCCNGGA 1 cut(s) 4830
PinAI ACCGGT 1 cut(s) 3535
Ple19I CGATCG 1 cut(s) 3670
PleI GAGTC 8 cut(s) 568, 935, 1718, 2075, 2631, 4232, 5369, 6789
PmaCI CACGTG 2 cut(s) 4703, 6419
PmlI CACGTG 2 cut(s) 4703, 6419
PpsI GAGTC 8 cut(s) 568, 935, 1718, 2075, 2631, 4232, 5369, 6789
Ppu21I YACGTR 3 cut(s) 294, 4703, 6419
PpuMI RGGWCCY 2 cut(s) 1293, 1747
PscI ACATGT 2 cut(s) 970, 2831
PsiI TTATAA 3 cut(s) 594, 1644, 4050
Psp5II RGGWCCY 2 cut(s) 1293, 1747
Psp6I CCWGG 6 cut(s) 1151, 2892, 3183, 6178, 6429, 6468
PspCI CACGTG 2 cut(s) 4703, 6419
PspFI CCCAGC 2 cut(s) 1078, 4375
PspGI CCWGG 6 cut(s) 1151, 2892, 3183, 6178, 6429, 6468
PspOMI GGGCCC 2 cut(s) 1461, 4758
PspPI GGNCC 7 cut(s) 1293, 1461, 1462, 1747, 3161, 4758, 4759
PspPPI RGGWCCY 2 cut(s) 1293, 1747
PstI CTGCAG 2 cut(s) 2293, 6194
PvuI CGATCG 1 cut(s) 3670
PvuII CAGCTG 2 cut(s) 275, 3578
RsaI GTAC 9 cut(s) 615, 981, 2369, 2635, 3398, 3912, 5651, 5926, 6647
RsaNI GTAC 9 cut(s) 614, 980, 2368, 2634, 3397, 3911, 5650, 5925, 6646
RseI CAYNNNNRTG 5 cut(s) 606, 975, 2645, 2738, 6029
SapI GCTCTTC 4 cut(s) 2355, 3917, 4405, 5637
Sau96I GGNCC 7 cut(s) 1293, 1461, 1462, 1747, 3161, 4758, 4759
ScaI AGTACT 2 cut(s) 2635, 5926
SchI GAGTC 8 cut(s) 569, 936, 1718, 2076, 2632, 4233, 5370, 6790
ScrFI CCNGG 9 cut(s) 733, 1153, 2894, 3185, 4450, 4832, 6180, 6431, 6470
SduI GDGCHC 5 cut(s) 1120, 1465, 1720, 4279, 4762
SfcI CTRYAG 2 cut(s) 2289, 6190
SfuI TTCGAA 1 cut(s) 3657
SinI GGWCC 3 cut(s) 1293, 1747, 3161
SmiMI CAYNNNNRTG 5 cut(s) 606, 975, 2645, 2738, 6029
SmlI CTYRAG 9 cut(s) 92, 656, 1091, 2198, 2302, 2620, 2725, 4118, 5495
SmoI CTYRAG 9 cut(s) 92, 656, 1091, 2198, 2302, 2620, 2725, 4118, 5495
SphI GCATGC 1 cut(s) 1563
SsiI CCGC 6 cut(s) 56, 1361, 1776, 2562, 5376, 5452
SspI AATATT 3 cut(s) 346, 2431, 5713
StyD4I CCNGG 9 cut(s) 731, 1151, 2892, 3183, 4448, 4830, 6178, 6429, 6468
StyI CCWWGG 4 cut(s) 1863, 2646, 3223, 5910
TaaI ACNGT 8 cut(s) 2753, 3047, 3349, 3415, 5849, 6044, 6598, 6664
TaiI ACGT 6 cut(s) 147, 243, 296, 3141, 4705, 6421
TatI WGTACW 6 cut(s) 979, 2367, 2633, 3396, 5649, 5924
TauI GCSGC 3 cut(s) 2565, 5379, 5454
TseFI GTSAC 1 cut(s) 133
Tsp45I GTSAC 1 cut(s) 133
TspGWI ACGGA 7 cut(s) 65, 777, 1118, 1523, 1803, 4245, 4797
Vha464I CTTAAG 3 cut(s) 92, 656, 2725
VpaK11BI GGWCC 3 cut(s) 1293, 1747, 3161
XbaI TCTAGA 2 cut(s) 3133, 6379
XceI RCATGY 6 cut(s) 974, 1563, 2374, 2835, 5008, 5656
XcmI CCANNNNNNNNNTGG 1 cut(s) 3820
XmiI GTMKAC 5 cut(s) 330, 930, 1432, 4227, 4729
XmnI GAANNNNTTC 4 cut(s) 113, 876, 5029, 6371
ZraI GACGTC 1 cut(s) 241
ZrmI AGTACT 2 cut(s) 2635, 5926
Zsp2I ATGCAT 6 cut(s) 1565, 1806, 2383, 3854, 4862, 5103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.